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Letteratura scientifica selezionata sul tema "PRRS-V"

Autore: Grafiati

Pubblicato: 19 dicembre 2024

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Articoli di riviste sul tema "PRRS-V"

Kosinova, E., e I. Psikal. "Restriction fragment length polymorphism of ORF6 and ORF7 genes of porcine reproductive and respiratory syndrome virus (PRRSV) vaccine strains registered in the CzechRepublic". Veterinární Medicína 51, No. 8 (27 marzo 2012): 414–22. http://dx.doi.org/10.17221/5565-vetmed.

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Restriction fragment length polymorphism (RFLP) of open reading frames 6 and 7 was applied to comparative genetic analysis of live attenuated vaccine strains (Amervac-PRRS/A3, Porcilis PRRS, Ingelvac PRRS) of porcine reproductive and respiratory syndrome virus (PRRSV), registered in the Czech Republic, six field viruses (L-588, L-1606, L-2053, L-3305, L-6558, L-6791), and three PRRSV local field isolates (CAMP V-502, CAMP V-503, VOS 2878) found in pig herds in the Czech Republic and Slovak Republic. The set of restriction enzymes Hae II, Alu I and BsaJ I allowed the differentiation of local field isolates, field viruses of PRRS, and vaccine strains of the European genotype from North American genotype, but could also distinguish between viruses of the same genotype. Five different RFLP patterns were obtained from twelve examined PRRS viruses by combination of the above restriction enzymes. RFLP code 1-1-1 was the most frequent digestion pattern within all PRRS field viruses (L-588, L-1606, L-2053, L-3305, L-6558, L-6791), CAMP V-502 isolate and vaccine strain Porcilis PRRS, which is suggestive of higher antigenic identity among the compared viruses. In the North American types (Ingelvac PRRS vaccine strain and VOS 2878 isolate), homogeneity in restriction patterns (code 2-x-4) was recorded. These studies indicate that PCR-based RFLP analysis of ORF6 and ORF7 of genes might be a suitable tool in epidemiological studies of PRRSV, similarly to the studies based on genetic analysis of ORF5 gene.

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Bertram, Mark J., Steve J. Kitt e Keith Kinsely. "PSVI-14 Impact of Bacillus Subtilis and Bacillus Licheniformis on Weaning Weight and Subsequent Performance, Health and Immune Parameters When Challenged with PRRSV with and Without PRRS Vaccination". Journal of Animal Science 100, Supplement_2 (12 aprile 2022): 160–61. http://dx.doi.org/10.1093/jas/skac064.274.

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Abstract A study was conducted examining the impact of feeding BioPlus 2B to PRRSV naïve sows during gestation and lactation on litter performance and on post-weaning performance when challenged with PRRSV in vaccinated and unvaccinated pigs. A total of 108 sows were allotted by parity and received a control diet (C) or BioPlus 2B (B2B) supplemented diet beginning a minimum of 31 d prior to farrowing through weaning. Pig weight was measured at birth and 17 d of age. At weaning, a subset of 200 pigs from each treatment were transported to a nursery and randomly allotted within dietary treatment on day 1 to a control (-V) or Ingelvac PRRS MLV vaccine (+V), establishing treatments of C+V, C-V, B2B+V, and B2B-V. On d 21, all pigs were intramuscularly challenged with a 1-18-2 PRRS virus. Growth rate and feed intake were monitored, serological and immune measures were recorded on d 21, 28, 35 and 42, post-weaning and on d 42 gross lung lesions were recorded. Pigs from sows receiving B2B tended (P < 0.10) to weigh more at 17 d of age and 21 d post-weaning (Table 1). Prior to challenge, there were no performance differences among treatments. Post challenge, there was a V x B2B interaction with C+V, B2B+V and B2B-V pigs having higher ADG and FG (P < .05) compared with C-V. On day 42, pigs fed B2B displayed improved lung lesion scores (P < 0.05) regardless of vaccine status. PRRSV viremia, measured by PCR and qPCR decreased more quickly when pigs consumed B2B (P < 0.05). Additionally, B2B modulated PRRSV specific T-cell interferon gamma production (P < 0.05). In conclusion, pigs consuming B2B from sows fedB2B were heavier at weaning and when subjected to a PRRSV challenge post-weaning, grew faster, were more efficient, and had reduced gross lung lesions, likely due to changes in immune function.

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Indik, S., e L. Valíček. "Differentiation of porcine reproductive and respiratory syndrome virus European vaccine strains from Czech field isolates by restriction fragment length polymorphism analysis of ORF5 gene". Veterinární Medicína 47, No. 10 - 11 (30 marzo 2012): 295–301. http://dx.doi.org/10.17221/5838-vetmed.

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Restriction fragment length polymorphism (RFLP) analysis of open reading frame 5 was developed for typing of Czech strains of porcine reproductive and respiratory syndrome virus (PRRSV). The set of restriction enzymes Acc I, Hae II and SnaB I allowed the differentiation of heterogeneous Czech strains of PRRSV clustered separately in the phylogenetic tree. The high-passage strain V-502 (164) was also differentiated from its parent strain V-502. The same restriction enzymes could distinguish the European-type vaccine strains Porcilis PRRS and Pyrsvac-183, registered inCzechRepublic, from the Czech field isolates. The published ORF5 nucleotide sequences allowed us to presume that it will also be possible to distinguish most of European field strains from vaccine strains. PCR-based RFLP analysis can become a valuable tool in epidemiological studies of PRRSV inEurope.

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Torricelli, Martina, Anna Fratto, Marcella Ciullo, Carla Sebastiani, Chiara Arcangeli, Andrea Felici, Samira Giovannini, Francesca Maria Sarti, Marco Sensi e Massimo Biagetti. "Porcine Reproductive and Respiratory Syndrome (PRRS) and CD163 Resistance Polymorphic Markers: What Is the Scenario in Naturally Infected Pig Livestock in Central Italy?" Animals 13, n. 15 (31 luglio 2023): 2477. http://dx.doi.org/10.3390/ani13152477.

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Porcine Reproductive and Respiratory Syndrome (PRRS) caused by the PRRS virus affects farmed pigs worldwide, causing direct and indirect losses. The most severe manifestations of PRRS infection are observed in piglets and pregnant sows. The clinical outcome of the infection depends on the PRRSV strain’s virulence, the pregnancy state of the female, environmental factors, the presence of protective antibodies due to previous infections, and the host’s genetic susceptibility. The latter aspect was investigated in this study, in particular, evaluating the most significant polymorphisms (SNPs) of the CD163 gene in slaughtered pigs reared in Central Italy. Total RNAs were extracted from 377 swine samples and subjected to RT-PCR targeted to the CD163 gene, followed by sequencing analysis. Contextually, the viral RNA was detected by RT-qPCR in order to phenotypically categorize animals into infected and not infected. In particular, 36 haplotypes were found, and their frequencies ranged from 0.13% to 35.15%. There were 62 resulting genotypes, three of which were associated with a putative resistance to the disease. Both the haplotypes and genotypes were inferred by PHASE v.2.1 software. To the best of our knowledge, this type of investigation was conducted for the first time on pig livestock distributed in different regions of Central Italy. Thus, the obtained findings may be considered very important since they add useful information about swine genetic background in relation to PRRS infection, from the perspective of adopting Marker-Assisted Selection (MAS) as a possible and alternative strategy to control this still widespread disease.

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Wilcock, Pete, Kiah M. Berg, Gustavo Cordero e Anthony Russo. "PSII-14 The Effect of Increasing Soluble Dietary Fiber and the Addition of a Stimbiotic in Gestation on Sow Productivity in a Prrs and Non-Prrs Challenge". Journal of Animal Science 101, Supplement_2 (28 ottobre 2023): 290–91. http://dx.doi.org/10.1093/jas/skad341.331.

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Abstract This sow study evaluated the effect that sugar beet pulp (SBP), a soluble fiber source, and a stimbiotic (Signis; AB Vista) can have on sow productivity and health when fed in gestation. A stimbiotic is an additive that stimulates fiber-degrading microbiome, resulting in an increase in fiber fermentability even though the additive itself contributes limited small chain fatty acid production. A commercial gestation barn with two feeding lines was utilized to feed sows (n = 2173) two dietary treatments from d 30 to d 90; a corn, soy, soy hulls with (HSDF) or without (LSDF) SBP (50 kg/t) plus stimbiotic (100 g/t) and from d 90 to entering the farrowing pen the LSDF diet with or without a stimbiotic (100 g/day). Sows were fed 2.0 kg per day providing the following fiber intake levels/day, HDSF: TDF; 266g, SDF 55g, IDF, 211g and LDSF: TDF; 240g, SDF 40g, IDF, 200g. The following variables were measured; total born, live born, still born, mummies, pre-weaning mortality, pigs weaned per sow, prolapse rate and sow mortality/culls. A proportion of sows (n = 81) were moved to a GESTAL system for lactation and intake recorded per gestation treatment. During the study the farm became PRRS positive and any sows that farrowed while the farm was PRRS positive were identified as PRRS positive sows and included as a factor. Data were analyzed with ANOVA evaluating the effects of fiber, parity, and PRRS using the fit model platform in JMP16. Significant treatment means were separated using Student’s t-tests, with significance accepted at P ≤ 0.05. Results showed that for born alive there was an interaction between treatment and parity (P =0.02) and treatment and PRRS (P < 0.05) with the HDSF diet increasing born alive in parity 1 (+1.30 pigs; P = 0.03) and parity 2 (+0.71 pigs; P = 0.08) with no effect in parity 3+. HDSF improved born alive in non PRRS pigs (+1.01 pigs; P < 0.01) and had no effect in PRRS pigs. There was a tendency for HDSF program to reduce stillborn (8.8% v 7.4%; P = 0.09) but had no effect on mummies. There was a tendency to increase in pigs weaned with the HSDF treatment (9.15 v 8.86; P = 0.07) with no effect on sow mortality/culls. There was no effect of treatment on prolapses. On the sub-sample of sows recorded in lactation those sows (Parity 2+) that had been on the HSDF in gestation showed an increased ADFI (5.36 kg v 6.02 kg; P = 0.01) compared with the LSDF. It can be concluded that sow productivity can be improved by increasing the fermentative capacity of the gestation feed through the combination of adding a soluble fiber such as SBP along with a stimbiotic.

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ROLINEC, Michal, Daniel Bíro, Branislav Gálik, Milan Šimko e Miroslav Juráček. "IMMUNOGLOBULINS IN COLOSTRUM OF SOWS WITH PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME - PRRS". Journal of Central European Agriculture 13, n. 2 (2012): 301–8. http://dx.doi.org/10.5513/jcea01/13.2.1049.

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Graham, Simon P., Yuen-Ki Cheong, Summer Furniss, Emma Nixon, Joseph A. Smith, Xiuyi Yang, Rieke Fruengel et al. "Antiviral Efficacy of Metal and Metal Oxide Nanoparticles against the Porcine Reproductive and Respiratory Syndrome Virus". Nanomaterials 11, n. 8 (20 agosto 2021): 2120. http://dx.doi.org/10.3390/nano11082120.

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Abstract (sommario):

Porcine reproductive and respiratory syndrome viruses (PRRSV) are responsible for one of the most economically important diseases affecting the global pig industry. On-farm high-efficiency particulate air (HEPA) filtration systems can effectively reduce airborne transmission of PRRSV and the incidence of PRRS, but they are costly, and their adoption is limited. Therefore, there is a need for low-cost alternatives, such as antimicrobial filters impregnated with antiviral nanoparticles (AVNP). During the past 10 years, tailored intermetallic/multi-elemental AVNP compositions have demonstrated effective performance against human viruses. In this study, a panel of five AVNP was evaluated for viricidal activity against PRRSV. Three AVNP materials: AVNP2, copper nanoparticles (CuNP), and copper oxide nanoparticles (CuONP), were shown to exert a significant reduction (>99.99%) in virus titers at 1.0% (w/v) concentration. Among the three, CuNP was the most effective at lower concentrations. Further experiments revealed that AVNP generated significant reductions in viral titers within just 1.5 min. For an optimal reduction in viral titers, direct contact between viruses and AVNP was required. This was further explained by the inert nature of these AVNP, where only negligible leaching concentrations of Ag/Cu ions (0.06–4.06 ppm) were detected in AVNP supernatants. Real-time dynamic light scatting (DLS) and transmission electron microscopic (TEM) analyses suggested that the mono-dispersive hydrodynamic behavior of AVNPs may have enhanced their antiviral activity against PRRSV. Collectively, these data support the further evaluation of these AVNP as candidate nanoparticles for incorporation into antimicrobial air-filtration systems to reduce transmission of PRRSV and other airborne pathogens.

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Wang, Jingjing, Zhaohai Du, Xuehan Huo, Juan Zhou, Yu Chen, Jingxia Zhang, Ao Pan, Xiaoyang Wang, Furong Wang e Jun Zhang. "Genome-wide analysis of PRR gene family uncovers their roles in circadian rhythmic changes and response to drought stress in Gossypium hirsutum L." PeerJ 8 (25 settembre 2020): e9936. http://dx.doi.org/10.7717/peerj.9936.

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Background The circadian clock not only participates in regulating various stages of plant growth, development and metabolism, but confers plant environmental adaptability to stress such as drought. Pseudo-Response Regulators (PRRs) are important component of the central oscillator (the core of circadian clock) and play a significant role in plant photoperiod pathway. However, no systematical study about this gene family has been performed in cotton. Methods PRR genes were identified in diploid and tetraploid cotton using bioinformatics methods to investigate their homology, duplication and evolution relationship. Differential gene expression, KEGG enrichment analysis and qRT-PCR were conducted to analyze PRR gene expression patterns under diurnal changes and their response to drought stress. Results A total of 44 PRR family members were identified in four Gossypium species, with 16 in G. hirsutum, 10 in G. raimondii, and nine in G. barbadense as well as in G. arboreum. Phylogenetic analysis indicated that PRR proteins were divided into five subfamilies and whole genome duplication or segmental duplication contributed to the expansion of Gossypium PRR gene family. Gene structure analysis revealed that members in the same clade are similar, and multiple cis-elements related to light and drought stress response were enriched in the promoters of GhPRR genes. qRT-PCR results showed that GhPRR genes transcripts presented four expression peaks (6 h, 9 h, 12 h, 15 h) during 24 h and form obvious rhythmic expression trend. Transcriptome data with PEG treatment, along with qRT-PCR verification suggested that members of clade III (GhPRR5a, b, d) and clade V (GhPRR3a and GhPRR3c) may be involved in drought response. This study provides an insight into understanding the function of PRR genes in circadian rhythm and in response to drought stress in cotton.

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Liu, Wenguang, Bing Liu, Gege Zhang, Huixia Jia, Yang Zhang, Xitong Cen, Gaoyou Yao e Maoxian He. "Molecular and Functional Characterization of a Short-Type Peptidoglycan Recognition Protein, Ct-PGRP-S1 in the Giant Triton Snail Charonia tritonis". International Journal of Molecular Sciences 23, n. 19 (21 settembre 2022): 11062. http://dx.doi.org/10.3390/ijms231911062.

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Peptidoglycan recognition proteins (PGRPs) are a family of pattern recognition receptors (PRRs) involved in host antibacterial responses, and their functions have been characterized in most invertebrate and vertebrate animals. However, little information is available regarding the potential function of PGRPs in the giant triton snail Charonia tritonis. In this study, a short-type PGRP gene (termed Ct-PGRP-S1) was identified in C. tritonis. Ct-PGRP-S1 was predicted to contain several structural features known in PGRPs, including a typical PGRP domain (Amidase_2) and Src homology-3 (SH3) domain. The Ct-PGRP-S1 gene was constitutively expressed in all tissues examined except in proboscis, with the highest expression level observed in the liver. As a typical PRR, Ct-PGRP-S1 has an ability to degrade peptidoglycan (PGN) and was proven to have non-Zn2+-dependent amidase activity and antibacterial activity against Vibrioalginolyticus and Staphylococcus aureus. It is the first report to reveal the peptidoglycan recognition protein in C. tritonis, and these results suggest that peptidoglycan recognition protein Ct-PGRP-S1 is an important effector of C. tritonis that modulates bacterial infection resistance of V. alginolyticus and S. aureus, and this study may provide crucial basic data for the understanding of an innate immunity system of C. tritonis.

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Kyutoku, Fumiaki, Takashi Yokoyama e Katsuaki Sugiura. "Genetic Diversity and Epidemic Types of Porcine Reproductive and Respiratory Syndrome (PRRS) Virus in Japan from 2018 to 2020". Epidemiologia 3, n. 2 (3 giugno 2022): 285–96. http://dx.doi.org/10.3390/epidemiologia3020022.

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To clarify the genetic diversity of the porcine reproductive and respiratory syndrome virus (PRRSV) in Japan in recent years, we determined the nucleotide sequence of open reading frame 5 of 2482 PRRSV sequences obtained from samples collected from pigs between January 2018 and December 2020. As a result of molecular phylogenetic analysis, Cluster II represented the largest proportion (44.9–50.6%) throughout the study period, followed by Cluster IV (34.0–40.8%), Cluster III (7.8–12.1%), Cluster I (3.1–6.7%), and Cluster V (0.1–0.2%). The relative distributions between Clusters varied between geographic regions and between years: in 2018, Cluster II was the most prevalent in all regions. In 2019, Cluster II was dominant in the Hokkaido and Tohoku regions, while in other regions Cluster IV was dominant. In 2020, Cluster IV was dominant in the Kanto/Tosan and Kyushu/Okinawa regions, whilst in other regions Cluster II was predominant. Compared with a previous study, the proportions of genome sequences classified in Clusters II and IV significantly increased (p = 0.042 and 0.018, respectively) and those classified in Cluster III significantly decreased (p < 0.01). The widespread use of live attenuated vaccines using strains that belong to Cluster II might have accounted for these changes in the relative distribution between Clusters.

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Più fonti

Tesi sul tema "PRRS-V"

Dráber, Peter. "Charakterizace transmembránového adaptorového proteinu PRR7 v buňkách imunitního systému". Master's thesis, 2007. http://www.nusl.cz/ntk/nusl-369014.

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Hrdinka, Matouš. "Úloha membránových mikrodomén a transmembránových adaptorových proteinů PRR7 a SCIMP v regulaci imunoreceptorové signalizace". Doctoral thesis, 2012. http://www.nusl.cz/ntk/nusl-312112.

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Dissertation summary The role of membrane microdomains and transmembrane adaptor proteins PRR7 and SCIMP in the regulation of immunoreceptor signaling Matouš Hrdinka How do the plasma membrane microdomains and transmembrane adaptor proteins (TRAPs) influence the outcome of immunoreceptor signaling? These have been the important questions of molecular immunology. In spite of the years of intensive research, these problems remain incompletely understood. The plasma membrane is a highly dynamic heterogeneous bilayer spontaneously organized into microdomains of various size, composition, and lifetime. The lipid rafts are one example of such microdomains and have been implicated in many biological processes, including immunoreceptor signaling. Because rafts are enriched in many signaling proteins, they are believed to function as platforms for signal initiation and propagation. The TRAPs are important organizers and regulators of immunoreceptor signaling. For example, LAT is indispensable in T cell receptor (TCR) signaling and T cell development, PAG for the regulation of Src family tyrosine kinases (SFKs), and NTAL is a multifunctional negative and positive regulator. The presence of these TRAPs in lipid rafts seems to be crucial for their functions, however, is still a matter of debate. Moreover, other so far...

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