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Tesi sul tema "Proteomics"

Autore: Grafiati

Pubblicato: 4 giugno 2021

Ultima modifica: 25 luglio 2025

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1

Miller, V. F. "Neuroretinal proteomics." Thesis, Queen's University Belfast, 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.411748.

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2

Franchin, Cinzia. "Mass Spectrometry-Based Quantitative Proteomics to Study Proteomes, Phosphoproteomes and Interactomes." Doctoral thesis, Università degli studi di Padova, 2012. http://hdl.handle.net/11577/3422169.

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Over the past few years, mass spectrometry-based proteomics has been widely applied to the most diverse fields of biochemistry, biomedicine and biology, and several approaches have been developed to allow absolute and relative quantification of proteins in very complex mixtures. During my PhD, I have conducted three main studies, taking advantage of different quantitative proteomics techniques. In particular SILAC and iTRAQ approaches have been exploited to investigate the calcification process induced by endotoxin in clonal interstitial aortic valve cells, while SILAC and label-free quantitat

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3

Walther, Dirk Martin. "Analysis of aging by quantitative proteomics and mitochondrial organellar proteomics." Diss., Ludwig-Maximilians-Universität München, 2012. http://nbn-resolving.de/urn:nbn:de:bvb:19-174637.

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4

Ismail, Marcus. "Blodplasmahantering för proteomics." Thesis, Mälardalen University, School of Sustainable Development of Society and Technology, 2007. http://urn.kb.se/resolve?urn=urn:nbn:se:mdh:diva-5485.

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5

Stone, Helen Marie. "Proteomics in COPD." Thesis, University of Birmingham, 2017. http://etheses.bham.ac.uk//id/eprint/7565/.

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In alpha-1-antitrypsin deficiency (A1ATD) there is excess neutrophil elastase activity, resulting in proteolytic destruction of the lung parenchyma. I hypothesised that the peptide fragments of proteins present in the lung might be detectable in plasma by mass spectrometry and that they might be useful biomarkers of disease activity and treatment efficacy. Calcium ionophore, neutrophil elastase and proteinase 3 were added to plasma from patients with A1ATD to create an in vitro model of the destructive processes. MALDI-based peptide profiling of plasma from patients pre and post treatment with

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6

Le, Thao Thi. "Aptamers for proteomics." Thesis, Imperial College London, 2008. http://hdl.handle.net/10044/1/1385.

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Changes in post-translation modifications are very important in the regulation of biological processes. Many modifications occur at very low levels, resulting in a low-abundance of the modified proteins in cells, and therefore assessing those modifications is not an easy task. Modern proteomics needs improved methods for identifying such changes. In this thesis, we focus on generating aptamers that can bind phosphoproteins with high affinities and therefore would be able to detect even low-abundance proteins. Aptamers are short sequences of nucleic acids that can be selected from libraries thr

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7

Lang, Alastair Michael. "Developing tissue proteomics : differential in gel electrophoresis in biomarker discovery and proteomic degradation." Thesis, University of Glasgow, 2013. http://theses.gla.ac.uk/4642/.

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The field of proteomics and functional genomics has developed steadily since the completion of the human genome project. The wealth of genomic information and the pace at which it was compiled was astounding. Proteomics, despite considerable effort, on the other hand has not seen quite the same pace of development. The progress being considerably hindered by the lack of an amplification process and the relative complexity of the proteome in comparison to the genome. These intrinsic difficulties have led to the sensitivity of proteomic techniques being pushed closer to physical limits. There is

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8

Culwell, Thomas Franklin. "Study of the reproducibility of proteomics methods and variability of fruit fly proteomes /." Diss., CLICK HERE for online access, 2008. http://contentdm.lib.byu.edu/ETD/image/etd2252.pdf.

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9

Culwell, Thomas Franklin. "Study of the Reproducibility of Proteomics Methods and Variability of Fruit Fly Proteomes." BYU ScholarsArchive, 2007. https://scholarsarchive.byu.edu/etd/1232.

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The reliability of biomarker discovery by means of proteomics has been called into question. It was speculated that "background noise" variation resulting from differences in preparation and handling of samples and proteome dynamics may mask subtle, yet important, differences due to the biological condition. Little is understood about complex proteomes and their variability. A critical aspect of proteomic biomarker research that is largely unexplored is the comparative reproducibility of certain methods such as two-dimensional gel electrophoresis and liquid chromatography/mass spectrometry. I

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10

Svensson, Marcus. "Neuropeptidomics expanding proteomics downwards /." Doctoral thesis, Uppsala : Uppsala universitet, Fakultetsövergripande enheter, Acta Universitatis Upsaliensis, 2007. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-7465.

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11

Dekker, Leendert Johannes Marinus. "Proteomics of body fluids." [S.l.] : Rotterdam : [The Author] ; Erasmus University [Host], 2007. http://hdl.handle.net/1765/10549.

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12

Gangadharan, Bevin. "Proteomics in viral disease." Thesis, University of Oxford, 2006. http://ora.ox.ac.uk/objects/uuid:c66c53ed-a824-4f99-8f2b-d2bc65a984c7.

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Abstract (sommario):

The separation, identification, and characterisation of the proteins present in a tissue or biological sample is called ‘proteomics’. This technique can be used for example to identify biomarkers and investigate signalling pathways. Increasingly, proteomics is being applied to the analysis of virus related samples; here two such examples are described. Presently there is no reliable non-invasive way of assessing liver fibrosis. Here a novel 2D-PAGE based proteomics study was used to identify potential fibrosis biomarkers. Serum from patients with varying degrees of hepatic scarring induced by

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13

Xia, Dong. "Proteomics of Toxoplasma gondii." Thesis, University of Liverpool, 2009. http://livrepository.liverpool.ac.uk/1276/.

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The Apicomplexan parasite Toxoplasma gondii is an obligate intracellular parasite. Infection by T .gondii causes the disease toxoplasmosis, which is one of the most prevalent parasitic diseases of animals and humans. It has been 100 years since the first discovery of the parasite in 1908; research on T. gondii has been carried out in many scientific disciplines consistently expanding the understanding of this parasite. In the last ten years, the developments of EST, microarray, genome sequencing and continuing efforts towards genome annotation has centralized the focus of T. gondii research on

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14

Ottervald, Jan. "Proteomics in neurological disease." Stockholm, 2009. http://diss.kib.ki.se/2009/978-91-7409-729-0/.

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15

Niu, Lili. "Escherichia coli proteomics and bioinformatics." [College Station, Tex. : Texas A&M University, 2007. http://hdl.handle.net/1969.1/ETD-TAMU-1240.

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16

Yaseen, Mumtaz. "Proteomics of Acute Myeloid Leukemia:." Diss., lmu, 2007. http://nbn-resolving.de/urn:nbn:de:bvb:19-69882.

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17

Michalski, Annette. "Overcoming challenges of shotgun proteomics." Diss., lmu, 2012. http://nbn-resolving.de/urn:nbn:de:bvb:19-151295.

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18

Champion, Matthew Maurice. "Functional proteomics in Escherichia coli." Texas A&M University, 2005. http://hdl.handle.net/1969.1/3194.

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Abstract (sommario):

Cells respond to their environment with programmed changes in gene expression. Cataloging these changes at the protein level is key towards understanding the physiology of an organism. Multi-subunit and multi-protein complexes are also important and pathogenic and physiologic processes. In order to identify expressed proteins and potential protein complexes, we utilized a combination of non-denaturing chromatography and peptide mass fingerprinting. This approach allows us to identify the components of protein mixtures, as well as information lost in traditional proteomics, such as subunit asso

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19

Björling, Erik. "Databases for antibody-based proteomics." Doctoral thesis, KTH, Skolan för bioteknologi (BIO), 2008. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-9658.

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Humans are believed to have ~20,500 protein-coding genes andmuch effort has over the last years been put into the characterizationand localization of the encoded proteins in order to understand theirfunctions. One such effort is the Human Proteome Resource (HPR)project, started in Sweden 2003 with the aim to generate specificantibodies to each human protein and to use those antibodies toanalyze the human proteome by screening human tissues and cells.The work reported in this thesis deals with structuring of data fromantibody-based proteomics assays, with focus on the importance ofaggregating a

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20

Muir, Matthew Stewart. "Proteomics of the ovine cataract." Diss., Lincoln University, 2008. http://hdl.handle.net/10182/792.

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The lens of the eye needs to be completely transparent in order to allow all light entering the eye to reach the retina. This transparency is maintained by the highly ordered structure of the lens proteins the crystallins. Any disruption to the lens proteins can cause an opacity to develop which is known as cataract. During cortical cataract formation there is increased truncation of the lens crystallins. It is believed that overactivation of calcium-dependent cysteine proteases, the calpains, is responsible for the increased proteolysis of the crystallins seen during cataractogenesis. Within

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21

Boren, Mats. "Proteomics of barley starch granules /." Uppsala : Dept. of Plantbiology and Forest Genetics, Swedish University of Agricultural Sciences, 2005. http://epsilon.slu.se/2005107.pdf.

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22

Greninger, Alexander L. "Genomics and Proteomics of Picornaviruses." Thesis, University of California, San Francisco, 2013. http://pqdtopen.proquest.com/#viewpdf?dispub=3558423.

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<p> Viruses have long been noted to be composed simply of nucleic acid and protein. This thesis describes this confluence of science of viruses at the interface of genomics and proteomics. Chapter 2 describes the discovery of klassevirus, a new picornavirus in pediatric diarrhea. Chapter 3 shows that klassevirus is likely a human pathogen given the seroconversion of klassevirus-positive individuals against a klassevirus non-structural protein that is not present in the picornavirus virion. Subsequent work failed to obtain a culturable virus from klassevirus-positive stool samples, enabling the

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23

Bauer, Chris [Verfasser]. "Exploiting proteomics data / Chris Bauer." Berlin : Freie Universität Berlin, 2014. http://d-nb.info/104786231X/34.

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24

Johnson, Hannah. "New Approaches to Quantitative Proteomics." Thesis, University of Manchester, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.492739.

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Abstract (sommario):

Absolute quantitative proteomics typically relies on the use of stable isotope labelled internal standards introduced in a known amount. Comparative signal intensities of the labelled and unlabelled peptides allow the inference of protein concentration.

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25

Pusch, Miriam. "Proteomics of newly assembled chromatin." Diss., Ludwig-Maximilians-Universität München, 2014. http://nbn-resolving.de/urn:nbn:de:bvb:19-180964.

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26

Volchenboum, Samuel Louis. "Proteomics for cancer biomarker discovery." Thesis, Massachusetts Institute of Technology, 2007. http://hdl.handle.net/1721.1/39733.

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Thesis (S.M.)--Harvard-MIT Division of Health Sciences and Technology, 2007.<br>This electronic version was submitted by the student author. The certified thesis is available in the Institute Archives and Special Collections.<br>Includes bibliographical references (p. 51-54).<br>Background: If we are to successfully treat cancer, we must understand the biologic underpinnings in conjunction with early diagnosis. Genome-wide expression studies have advanced the research of many cancers. Nevertheless, understanding which genes are expressed in a tumor is not equivalent to knowing which proteins

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27

Jiang, Yanjie. "Approaches for Improved Positional Proteomics." ScholarWorks@UNO, 2013. http://scholarworks.uno.edu/td/1715.

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Positional proteomics is emerging as an attractive technique to characterize protein termini, which play important biological roles in cells. Even with the advances in past decades, there still are areas for improvement. This thesis focuses on improving data quality and assignment confidence in positional proteomics. A novel workflow was designed for the large-scale identification of protein N-terminal sequences. 4-sulfophenyl isothiocyanate (SPITC) is used for N-termini sulfonation; Upon higher energy collisional dissociation (HCD), SPITC peptides in electrospray ionization ESI generate predo

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28

Furlan, Cristina. "Quantitative proteomics of human chromatin." Thesis, University of Edinburgh, 2013. http://hdl.handle.net/1842/17992.

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The work presented in this thesis aims at unravelling human chromatin composition by quantitative proteomics to outline the functional and structural changes occurring during the life of human cells. Chromatin is the structure formed by proteins and RNAs interacting with the genetic material. At present, chromatin is not well defined. It is not easy to investigate either the composition of its constituent proteins or how this arrangement changes. We set out to analyse the chromatin composition changes occurring during the cell cycle. Our procedure couples a SILAC mass spectrometry-based approa

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29

Rolland, Catherine. "The proteomics of adipose tissue." Thesis, University of Aberdeen, 2005. http://digitool.abdn.ac.uk/R?func=search-advanced-go&find_code1=WSN&request1=AAIU200569.

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Obesity is a widespread disease both in the UK and worldwide and the prevalence is increasing in epidemic proportions. This increased weight and altered body fat distribution has brought about increased risk of comorbid disease such as type II diabetes, hypertension and cardiovascular disease. The aim of this study was to find novel proteins differentially expressed in subcutaneous and omental adipose tissue. To achieve this, approaches to resolve adipose tissue adequately using two-dimensional electrophoresis (2DE) were developed to allow the detection of differences in protein expression for

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30

Blakeley, Paul. "Computational proteomics for genome annotation." Thesis, University of Manchester, 2013. https://www.research.manchester.ac.uk/portal/en/theses/computational-proteomics-for-genome-annotation(e48dc673-a8b7-4c78-93ca-aab0eb28ec8a).html.

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The field of proteogenomics operates at the interface between proteomics and genomics, and has emerged during the past decade to exploit the vast quantities of high-throughput sequence data. A range of different proteogenomics approaches have been developed, which integrate mass spectrometry data with genome sequence data to provide empirical evidence for protein-coding genes. However, current methods may not be optimized as they do not fully consider the splicing complexity in eukaryotes and there is currently no best practice method. To address this, we investigate the level of proteomics su

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31

Salehi-Reyhani, Ali. "Tools for single cell proteomics." Thesis, Imperial College London, 2011. http://hdl.handle.net/10044/1/9280.

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Despite recent advances that offer control of single cells, in terms of manipulation and sorting and the ability to measure gene expression, the need to measure protein copy number remains unmet. Measuring protein copy number in single cells and related quantities such as levels of phosphorylation and protein-protein interaction is the basis of single cell proteomics. A technology platform to undertake the analysis of protein copy number from single cells has been developed. The approach described is ‘all-optical’ whereby single cells are manipulated into separate analysis chambers using an op

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32

Björling, Erik. "Databases for antibody-based proteomics /." Stockholm : Bioteknologi, Kungliga Tekniska högskolan, 2008. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-9658.

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33

Reitz, Nila. "Data mining for phospho-proteomics." Pullman, Wash. : Washington State University, 2009. http://www.dissertations.wsu.edu/Thesis/Fall2009/N_Reitz_120409.pdf.

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Thesis (M.S. in computer science)--Washington State University, December 2009.<br>Title from PDF title page (viewed on Feb. 4, 2010). "School of Electrical Engineering & Computer Science." Includes bibliographical references (p. 49-54).

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34

Eriksson, Cecilia. "Affinity based proteomics research tools /." Stockholm : Skolan för bioteknologi, Kungliga Tekniska högskolan, 2009. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-11184.

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35

Bromilow, Sophie. "Proteomics in 'free-from' foods." Thesis, University of Manchester, 2018. https://www.research.manchester.ac.uk/portal/en/theses/proteomics-in-afreefroma-foods(5793f2a1-9552-46e1-92b4-2fce7fadaa27).html.

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Wheat is the most agronomically important crop with an annual production of approximately 680 million tonnes per year over the five year period of 2008-2012 (Shewry and Tatham 2016). Wheat typically contributes about 20% of the total calorie intake in Western Europe and between 50-70% in some countries in North Africa and in West and Central Asia. It is estimated that in order to meet the continuous growing global demand wheat production needs to increase by 50% by 2050. Wheat is most commonly consumed as bread, pasta and noodles however it is also used as a food ingredient in other types of f

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36

Margarucci, Luigi. "Chemical proteomics on ligand protein." Doctoral thesis, Universita degli studi di Salerno, 2011. http://hdl.handle.net/10556/141.

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2009 - 2010<br>The emerging field of mass spectrometry-based chemical proteomics provides a powerful instrument in the target discovery of bioactive small-molecules, such as drugs or natural products[1]. The identification of their macromolecular targets is required for a comprehensive understanding of their bio-pharmacological role and for unraveling their mechanism of action[1, 2]. Indeed, the target discovery of bioactive molecules endowed with intriguing pharmacological profiles is one of the main issues in the field of pharmaceutical sciences, since this is necessary for a rational develo

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37

Gibson, Frank. "The development of standards for proteomics research and a proteomics investigation of diabetic adipocyte models." Thesis, University of Newcastle Upon Tyne, 2007. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.445541.

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38

Walther, Dirk Martin [Verfasser], and Matthias [Akademischer Betreuer] Mann. "Analysis of aging by quantitative proteomics and mitochondrial organellar proteomics / Dirk Martin Walther. Betreuer: Matthias Mann." München : Universitätsbibliothek der Ludwig-Maximilians-Universität, 2012. http://d-nb.info/1058822098/34.

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39

Pujari, Goutam. "Current and future trends in proteomics (SELDI-TOF) in clinical diagnosis and clinical research." Thesis, Click to view the E-thesis via HKUTO, 2004. http://sunzi.lib.hku.hk/hkuto/record/B31972111.

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40

Razunguzwa, Trust T. "Development of microfluidic devices for proteomics." Morgantown, W. Va. : [West Virginia University Libraries], 2006. https://eidr.wvu.edu/etd/documentdata.eTD?documentid=4691.

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Thesis (Ph. D.)--West Virginia University, 2006.<br>Title from document title page. Document formatted into pages; contains xiv, 131 p. : ill. (some col.). Includes abstract. Includes bibliographical references.

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41

Handtke, Stefan [Verfasser]. "Proteomics of Bacillus pumilus / Stefan Handtke." Greifswald : Universitätsbibliothek Greifswald, 2016. http://d-nb.info/1088768849/34.

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42

NAVARRO, JOSÉ FERNANDEZ. "Protein Level Probabilitiesfor Shotgun Proteomics Experiments." Thesis, KTH, Skolan för datavetenskap och kommunikation (CSC), 2013. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-137425.

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Abstract (sommario):

There exist many techniques to perform the analysis of proteins. Current Shotgun Proteomics based methods assign peptide level scores to peptide-spectrum matches obtained by matching observed spectra against a database of theoretically generated spectra from a set of known proteins. This set of peptide-spectrum matches is ranked according to a score based on the quality of the match. Subsequently, the candidate present proteins can be inferred from the confidently identified peptides. This process seems straightforward and out of the box, however, it has some notable weaknesses. The imperfecti

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43

Al, Lawati Haider A. J. "Toward a microfluidic system for proteomics." Thesis, University of Hull, 2007. http://hydra.hull.ac.uk/resources/hull:94.

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There has been much interest in the application of microfluidic systems to proteomics in recent years. This is because of the many unique advantages afforded by the reduced dimensions of microfluidic systems compared to classical methods. The reduction in the reaction vessel dimensions leads to a high degree of control, higher sensitivity, better selectivity and reduced analysis time. Initial experimental results have shown the possibility for developing a novel approach for proteomic analysis. A highly efficient protein digestion microdevice was fabricated using commercially available immobil

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44

Falk, Ronny. "Systems enabling antibody-mediated proteomics research." Doctoral thesis, Stockholm, 2006. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-4025.

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45

Grossmann, Jonas Tobias. "New strategies in proteomics data analysis." kostenfrei, 2007. http://e-collection.ethbib.ethz.ch/view/eth:29742.

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46

Elghawanmeh, Omar. "Proteomics characterization of the cardiac sarcolemma." Thesis, McGill University, 2006. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=99339.

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Abstract (sommario):

As the plasma membrane of the cardiac muscle cell (sarcolemma) plays a major role in the cardiac physiology and homeostasis, the aim of this study is to identify using mass spectrometry (MS) and data base mining the integral and peripheral proteins that are making its composition and to uncover putative novel proteins and to ultimately reveal the differences in protein composition between the sarcolemma of control rat hearts and hearts submitted to ischemia and ischemia-reperfusion to find disease-specific markers. Toward this goal we have developed a new method to purify cardiac sarcolemma us

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47

Khan, Amir Ali. "Proteomics analysis of human stem cells." Thesis, University of Leeds, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.493771.

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Stem cells have two characteristics that make them unique from any other cell: they can renew themselves indefmitely and they can be differentiated into other cell types. The aim of this investigation was to employ proteomic techniques (2D-PAGE/mass spectrometry) to compare protein expression profiles between two embryonic cell lines (HI and Hues1) and between Huesl and a multipotent cell line (MSC).

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48

Wright, James Christopher. "Techniques to improve Cross-Species Proteomics." Thesis, University of Liverpool, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.510938.

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49

Hudson, Sian Rose. "Towards chemical tagging strategies for proteomics." Thesis, University of York, 2010. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.535039.

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50

Deng, Yi, and 鄧藝. "From neuroimaging to proteomics in schizophrenia." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2009. http://hub.hku.hk/bib/B43278516.

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