Articoli di riviste: "Propionibacterium"

1

CHAIA, ADRIANA PEREZ, AIDA PESCE de RUIZ HOLGADO e GUILLERMO OLIVER. "Peptide Hydrolases of Propionibacteria: Effect of pH and Temperature". Journal of Food Protection 53, n. 3 (1 marzo 1990): 237–40. http://dx.doi.org/10.4315/0362-028x-53.3.237.

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A comparative study of the activity of peptidases belonging to the four classic propionibacteria species cultured in milk was carried out at different values of pH and temperature. Leucine aminopeptidase and proline iminopeptidase showed greater activity in Propionibacterium freudenreichii than in the other species studied. With the single exception of Propionibacterium jensenii, the propionibacteria peptidase tested exhibited greater affinity for proline than for leucine-p-nitroanilide. Optimum temperature and pH in relation to the activity of both substrates varied according to the species under consideration.

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Șandru, Daniela Maria, e Magda Panaitescu. "THE ANTIBACTERIAL POTENTIAL OF ESSENTIAL OILS ON PROPIONIBACTERIUM GRANULOSUM STRAINS". Management of Sustainable Development 12, n. 2 (1 dicembre 2020): 21–24. http://dx.doi.org/10.54989/msd-2020-0008.

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Gram-positive bacteria Propionibacterium granulosum lives on human skin along with other propionibacteria on the skin, these bacteria are especially important to have healthy skin and occupy some ecological niches. These niches are populated by some pathogenic bacteria. Propionibacterium granulosum bacteria produce some fatty acids that have low molecular weight, bacteriocins and other substances that inhibit some bacteria. The aim of this research is to investigate the microbiological evolution of some essential oils on Propionibacterium granulosum strains. It is observed that the highest values are recorded when 20 µL of essential oil is used.

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Vyas, D., A. Alazzeh, S. M. McGinn, T. A. McAllister, O. M. Harstad, H. Holo e K. A. Beauchemin. "Enteric methane emissions in response to ruminal inoculation of Propionibacterium strains in beef cattle fed a mixed diet". Animal Production Science 56, n. 7 (2016): 1035. http://dx.doi.org/10.1071/an14801.

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The objective of this study was to test the efficacy of Propionibacterium strains to mitigate enteric methane (CH4) emissions in beef heifers fed a mixed diet. An experiment was conducted with 16 ruminally cannulated beef heifers fed a basal diet consisting of 60 : 40 barley silage : barley grain (DM basis). Treatments included: (1) Control, (2) Propionibacterium freudenreichii T114, (3) P. thoenii T159, and (4) P. freudenreichii T54. Strains (1 × 1011 colony forming units) were administered daily directly into the rumen before feeding. No treatment effects were observed for DM intake (P = 0.90), mean ruminal pH (P = 0.50) and total volatile fatty acids (P = 0.44). However, compared with the Control, proportions of individual volatile fatty acids changed with acetate being less with Propionibacterium T159 (P = 0.02), whereas ruminal isobutyrate (P < 0.01) and acetate : propionate ratio (P = 0.04) were greater with Propionibacterium T114. Total daily enteric CH4 production averaged 188 g/day and was not affected by Propionbacterium strains (P = 0.51). Methane yield averaged 22 g/kg of DMI intake and tended to be greater with Propionibacterium strains (P = 0.08). The relative abundance of total Propionibacteria was greater with the inoculation of Propionibacterium T159 relative to the Control heifers (P = 0.04). In conclusion, inoculation of Propionibacterium T159 decreased ruminal acetate proportion and Propionibacterium T114 increased acetate : propionate ratio. However, inoculated strains failed to lower total CH4 emissions possibly due to the inability of Propionibacterium strains to elevate ruminal propionate concentrations.

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Farrar, Mark D., Karen M. Howson, Richard A. Bojar, David West, James C. Towler, James Parry, Katharine Pelton e Keith T. Holland. "Genome Sequence and Analysis of a Propionibacterium acnes Bacteriophage". Journal of Bacteriology 189, n. 11 (30 marzo 2007): 4161–67. http://dx.doi.org/10.1128/jb.00106-07.

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ABSTRACT Cutaneous propionibacteria are important commensals of human skin and are implicated in a wide range of opportunistic infections. Propionibacterium acnes is also associated with inflammatory acne vulgaris. Bacteriophage PA6 is the first phage of P. acnes to be sequenced and demonstrates a high degree of similarity to many mycobacteriophages both morphologically and genetically. PA6 possesses an icosahedreal head and long noncontractile tail characteristic of the Siphoviridae. The overall genome organization of PA6 resembled that of the temperate mycobacteriophages, although the genome was much smaller, 29,739 bp (48 predicted genes), compared to, for example, 50,550 bp (86 predicted genes) for the Bxb1 genome. PA6 infected only P. acnes and produced clear plaques with turbid centers, but it lacked any obvious genes for lysogeny. The host range of PA6 was restricted to P. acnes, but the phage was able to infect and lyse all P. acnes isolates tested. Sequencing of the PA6 genome makes an important contribution to the study of phage evolution and propionibacterial genetics.

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DARILMAZ, DERYA ONAL, e YESIM GUMUSTEKIN. "Research on Some Factors Influencing Acid and Exopolysaccharide Produced by Dairy Propionibacterium Strains Isolated from Traditional Homemade Turkish Cheeses". Journal of Food Protection 75, n. 5 (1 maggio 2012): 918–26. http://dx.doi.org/10.4315/0362-028x.jfp-11-510.

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In this study, a total of 32 isolated strains and 5 reference strains of dairy propionibacteria were analyzed for acid and exopolysaccharide (EPS) production in skim milk and yeast extract–lactate broth (YEL) media in order to investigate the physiological background and preservative role of acid and EPS. The effects of final culture pH and optical density on acid and EPS production were also determined. On average, all strains produced more acid and reached lower final pH values in skim milk than in YEL medium. While the correlations obtained between the acid produced by propionibacterium strains and their final culture pH in skim milk medium were significant (P < 0.01), no correlations were found between optical density, final pH, and produced acid in YEL medium. Sixteen isolated and five reference strains of propionibacteria were tested further for the ability to produce propionic and acetic acids. On average, Propionibacterium freudenreichii subsp. shermanii and P. freudenreichii subsp. freudenreichii strains produced higher amounts of propionic and acetic acids than did Propionibacterium jensenii in YEL medium. The acid produced by these strains may be used as a preservative in the food industry for replacement or reduction of the increasing use of chemical additives. The EPS production by propionibacterium strains during growth in YEL medium was 72 to 168 mg/liter, while in skim milk it was 94 to 359 mg/liter. The monomer compositions of the EPSs formed by the six selected dairy propionibacteria strains were analyzed. The EPSs may have applications as food grade additives and viscosity-stabilizing agents.

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CHAIA, A. PEREZ, A. M. STRASSER DE SAAD, A. PESCE DE RUIZ HOLGADO e G. OLIVER. "Competitive Inhibition of Propionibacterium acidipropionici by Mixed Culturing with Lactobacillus helveticus". Journal of Food Protection 57, n. 4 (1 aprile 1994): 341–44. http://dx.doi.org/10.4315/0362-028x-57.4.341.

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Lactobacillus helveticus and Propionibacterium acidipropionici were grown in pure and mixed cultures in a complex medium to assess the associative interaction. The specific growth rates, substrate consumption coefficient, substrate utilization and product formation rates were determined in each case. Propionibacterium acidipropionici utilized glucose preferably when it grew in a medium containing a mixture of glucose and lactate. Its growth rate was higher on glucose than on lactate in pure culture. However, lactic acid was the substrate utilized by propionibacteria in the associative growth. The fast pH reduction produced by the growth of lactobacilli and the slow lactate utilization by propionibacteria in mixed culture determined the inhibition of propionic acid bacteria in associative growth.

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Faye, Therese, Thor Langsrud, Ingolf F. Nes e Helge Holo. "Biochemical and Genetic Characterization of Propionicin T1, a New Bacteriocin from Propionibacterium thoenii". Applied and Environmental Microbiology 66, n. 10 (1 ottobre 2000): 4230–36. http://dx.doi.org/10.1128/aem.66.10.4230-4236.2000.

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ABSTRACT A collection of propionibacteria was screened for bacteriocin production. A new bacteriocin named propionicin T1 was isolated from two strains of Propionibacterium thoenii. This bacteriocin shows no sequence similarity to other bacteriocins. Propionicin T1 was active against all strains of Propionibacterium acidipropionici, Propionibacterium thoenii, andPropionibacterium jensenii tested and also againstLactobacillus sake NCDO 2714 but showed no activity againstPropionibacterium freudenreichii. The bacteriocin was purified, and the N-terminal part of the peptide was determined with amino acid sequencing. The corresponding gene pctA was sequenced, and this revealed that propionicin T1 is produced as a prebacteriocin of 96 amino acids with a typical sec leader, which is processed to give a mature bacteriocin of 65 amino acids. An open reading frame encoding a protein of 424 amino acids was found 68 nucleotides downstream the stop codon of pctA. The N-terminal part of this putative protein shows strong similarity with the ATP-binding cassette of prokaryotic and eukaryotic ABC transporters, and this protein may be involved in self-protection against propionicin T1. Propionicin T1 is the first bacteriocin from propionibacteria that has been isolated and further characterized at the molecular level.

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Jamil, Khanssa Badie. "Synthesis of Environmental Bioplastic Polyhydroxyalkanoate (PHA) from Waste Glycerol, Palm Oil and Different Concentrations of Glucose by A New Strain Propionibacterium Sp." Iraqi Journal of Industrial Research 9, n. 2 (20 ottobre 2022): 175–86. http://dx.doi.org/10.53523/ijoirvol9i2id203.

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Propionibacteria have mainly been found in dairy products and on the skin of humans and animals. This genus classified as non-producing PHA but in this study we show that Propionibacterium sp. can produce PHA. A member of the genus Propionibacterium was isolated from soil. The isolate was identified based on its gram positive pleomorphic rods. Cells occur short chains, singly or in pairs, in V or Y configurations. On mineral salt agar colonies are semi-opaque, convex, glittering and often pigmented in orange in color, biochemical tests and 16SrDNA also done. The 16SrDNA analysis confirmed 85% identity to Propionibacterium (accession number NR 074675.1). The presence of 3HB was identified, based on the analysis of NMR. 13C and 1H NMR analysis confirmed that Propionibacterium sp was able to produce PHA.polymer. These confirm the results of GC. The isolate was then grown on media with waste glycerol (WG), palm oil, different concentration of glucose and one stage and two stages of cultivation. This lead to the conclusion that Propionibacterium is able to grow utilizing waste glycerol, palm oil and different concentration of glucose as the sole carbon source under limited conditions. The PHA content 3 (wt%) and the composition (mol%) and (86% 3HB) when used glucose 2% at frist stage cultivation. while the PHA content 13 (wt %) when used palm oil and 5 (wt%) when utilized (WG), as sole carbon source.

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McCubbin, Tim, R. Axayacatl Gonzalez-Garcia, Robin W. Palfreyman, Chris Stowers, Lars K. Nielsen e Esteban Marcellin. "A Pan-Genome Guided Metabolic Network Reconstruction of Five Propionibacterium Species Reveals Extensive Metabolic Diversity". Genes 11, n. 10 (23 settembre 2020): 1115. http://dx.doi.org/10.3390/genes11101115.

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Propionibacteria have been studied extensively since the early 1930s due to their relevance to industry and importance as human pathogens. Still, their unique metabolism is far from fully understood. This is partly due to their signature high GC content, which has previously hampered the acquisition of quality sequence data, the accurate annotation of the available genomes, and the functional characterization of genes. The recent completion of the genome sequences for several species has led researchers to reassess the taxonomical classification of the genus Propionibacterium, which has been divided into several new genres. Such data also enable a comparative genomic approach to annotation and provide a new opportunity to revisit our understanding of their metabolism. Using pan-genome analysis combined with the reconstruction of the first high-quality Propionibacterium genome-scale metabolic model and a pan-metabolic model of current and former members of the genus Propionibacterium, we demonstrate that despite sharing unique metabolic traits, these organisms have an unexpected diversity in central carbon metabolism and a hidden layer of metabolic complexity. This combined approach gave us new insights into the evolution of Propionibacterium metabolism and led us to propose a novel, putative ferredoxin-linked energy conservation strategy. The pan-genomic approach highlighted key differences in Propionibacterium metabolism that reflect adaptation to their environment. Results were mathematically captured in genome-scale metabolic reconstructions that can be used to further explore metabolism using metabolic modeling techniques. Overall, the data provide a platform to explore Propionibacterium metabolism and a tool for the rational design of strains.

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Moroder, Philipp, Andrej Trampuz e Markus Scheibel. "Propionibacterium". Journal of Bone and Joint Surgery 98, n. 24 (dicembre 2016): e112. http://dx.doi.org/10.2106/jbjs.16.00838.

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Zárate, Gabriela, Vilma Morata De Ambrosini, Adriana Perez Chaia e Silvia González. "Some factors affecting the adherence of probiotic Propionibacterium acidipropionici CRL 1198 to intestinal epithelial cells". Canadian Journal of Microbiology 48, n. 5 (1 maggio 2002): 449–57. http://dx.doi.org/10.1139/w02-036.

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Adhesion to the intestinal mucosa is generally considered an important property of probiotic microorganisms and has been related to many of their health benefits. This study investigated some factors that could affect or be involved in the adherence of Propionibacterium acidipropionici CRL 1198, a dairy strain with suggested probiotic effects and high adherence in vitro and in vivo to intestinal epithelial cells. In vitro adhesion of propionibacteria was decreased by gastric digestion but not affected by bile and pancreatic enzymes. Adherence was also decreased by pretreatment of bacterial cells with protease, sodium metaperiodate, and trichloroacetic acid, revealing that different features of the cell surface, like protein factors, carbohydrates, and teichoic acids, are involved in the process. Adherence to intestinal epithelial cells was enhanced by calcium and was dependent on other divalent cations. Adhesion to intestinal mucus was also demonstrated. The results should explain the metabolic effects in the host previously obtained with this strain and support the potential of Propionibacterium for development of new probiotics.Key words: propionibacteria, adhesion, probiotics.

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Massoud, Ramona, Kianoush Khosravi-Darani, Milad Golshahi, Sara Sohrabvandi e Amir Mohammad Mortazavian. "Assessment of Process Variables on Vitamin B12 Production in Fermented Dairy Product Including Propionic Acid". Current Nutrition & Food Science 16, n. 2 (14 febbraio 2020): 155–61. http://dx.doi.org/10.2174/1573401315666181204105601.

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The fermented dairy products produced by various microorganism’s activity provide valuable nutrients for human. Fermentation affects the physicochemical and organoleptic characteristics of foods as well as human health. In the present review, we describe the production of vitamin B12 in a fermented dairy product by Propionibacterium species. The effect of the process variables on vitamin B12 production in fermented dairy products e.g. pH, temperature, different carbon and nitrogen sources as well as the type and size of inoculum, the fermentation time and fermentation strategy, etc. are discussed. Finally, fermentation strategy, inoculum preparation of Propionibacterium freudenreichii subsp. Shermanii, methods of determining biomass and Vitamin B12 concentration are reviewed and their effects on vitamin B12 production are mentioned. Propionibacteria are so popular due to their unique characteristics such as being safe, convenient and economical. Among all the highest efficiency was observed by P. freudenreichii. To achieve this purpose, some appropriate situations need to be considered. The best carbon source for this bacterium to produce biomass is lactate, the suitable pH for growth of the Propionibacterium species is in the range of 6 to 7 and the fed-batch is most preferable for vitamin B12 production.

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Foligné, Benoît, Stéphanie-Marie Deutsch, Jérôme Breton, Fabien J. Cousin, Joëlle Dewulf, Michel Samson, Bruno Pot e Gwénaël Jan. "Promising Immunomodulatory Effects of Selected Strains of Dairy Propionibacteria as Evidenced In Vitro and In Vivo". Applied and Environmental Microbiology 76, n. 24 (22 ottobre 2010): 8259–64. http://dx.doi.org/10.1128/aem.01976-10.

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ABSTRACT Immunomodulatory properties of 10 dairy propionibacteria, analyzed on human peripheral blood mononuclear cells (PBMCs), revealed a highly strain-dependent induction of anti-inflammatory cytokine interleukin 10 (IL-10). Two selected strains of Propionibacterium freudenreichii showed a protective effect against two models of colitis in mice, suggesting a probiotic potential predicted by immune-based selection criteria for these cheese starter bacteria.

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Perry, A. L., e P. A. Lambert. "Propionibacterium acnes". Letters in Applied Microbiology 42, n. 3 (marzo 2006): 185–88. http://dx.doi.org/10.1111/j.1472-765x.2006.01866.x.

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Carneiro, Inês Marques, Ana Sousa Pereira, Sara Pinto, Filipa Prata, Cláudia C. Faria e José Gonçalo Marques. "Propionibacterium Acnes". Pediatric Infectious Disease Journal 37, n. 6 (giugno 2018): e168-e169. http://dx.doi.org/10.1097/inf.0000000000001786.

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Glenn, A. M., e C. M. Wood. "Propionibacterium acnes." BMJ 297, n. 6642 (16 luglio 1988): 201–2. http://dx.doi.org/10.1136/bmj.297.6642.201-d.

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ZÁRATE, GABRIELA, VILMA I. MORATA de AMBROSINI, ADRIANA PEREZ CHAIA e SILVIA N. GONZÁLEZ. "Adhesion of Dairy Propionibacteria to Intestinal Epithelial Tissue In Vitro and In Vivo". Journal of Food Protection 65, n. 3 (1 marzo 2002): 534–39. http://dx.doi.org/10.4315/0362-028x-65.3.534.

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Adhesion to the intestinal mucosa is a desirable property for probiotic microorganisms and has been related to many of their health benefits. In the present study, 24 dairy Propionibacterium strains were assessed with regard to their hydrophobic characteristics and their autoaggregation and hemagglutination abilities, since these traits have been shown to be indicative of adherence in other microorganisms. Six strains were further tested for their capacity to adhere to ileal epithelial cells in vitro and in vivo. The results of the study showed that propionibacteria were highly hydrophilic, and hemagglutination and autoaggregation were properties not commonly found among these microorganisms. No relationship was found between surface characteristics and adhesion ability, since hemagglutinating, autoaggregating, and nonautoaggregating bacteria were able to adhere to intestinal cells both in vitro and in vivo. Microscopic examination revealed that autoaggregating cells adhered in clusters, with adhesion being mediated by only a few bacteria, whereas the hemagglutinating and nonautoaggregating strains adhered individually or in small groups making contact with each epithelial cell with the entire bacterial surface. The in vitro assessment of adhesion was a good indication of the in vivo association of propionibacteria with the intestinal epithelium. Therefore, the in vitro method presented here should be valuable in screening routinely adhesive properties of propionibacteria for probiotic purposes. The adhesion ability of dairy propionibacteria would prolong their maintenance in the gut and increase the duration of their provision of beneficial effects in the host, supporting the potential of Propionibacterium in the development of new probiotic products.

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Treimo, Janneke, Gerd Vegarud, Thor Langsrud e Knut Rudi. "Use of DNA Quantification To Measure Growth and Autolysis of Lactococcus and Propionibacterium spp. in Mixed Populations". Applied and Environmental Microbiology 72, n. 9 (settembre 2006): 6174–82. http://dx.doi.org/10.1128/aem.00515-06.

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ABSTRACT Autolysis is self-degradation of the bacterial cell wall that results in the release of enzymes and DNA. Autolysis of starter bacteria, such as lactococci and propionibacteria, is essential for cheese ripening, but our understanding of this important process is limited. This is mainly because the current tools for measuring autolysis cannot readily be used for analysis of bacteria in mixed populations. We have now addressed this problem by species-specific detection and quantification of free DNA released during autolysis. This was done by use of 16S rRNA gene single-nucleotide extension probes in combination with competitive PCR. We analyzed pure and mixed populations of Lactococcus lactis subsp. lactis and three different species of Propionibacterium. Results showed that L. lactis subsp. lactis INF L2 autolyzed first, followed by Propionibacterium acidipropionici ATCC 4965, Propionibacterium freudenreichii ISU P59, and then Propionibacterium jensenii INF P303. We also investigated the autolytic effect of rennet (commonly used in cheese production). We found that the effect was highly strain specific, with all the strains responding differently. Finally, autolysis of L. lactis subsp. lactis INF L2 and P. freudenreichii ISU P59 was analyzed in a liquid cheese model. Autolysis was detected later in this cheese model system than in broth media. A challenge with DNA, however, is DNA degradation. We addressed this challenge by using a DNA degradation marker. We obtained a good correlation between the degradation of the marker and the target in a model experiment. We conclude that our DNA approach will be a valuable tool for use in future analyses and for understanding autolysis in mixed bacterial populations.

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NATH, K. R., e B. J. KOSTAK. "Etiology of White Spot Defect in Swiss Cheese Made from Pasteurized Milk". Journal of Food Protection 49, n. 9 (1 settembre 1986): 718–23. http://dx.doi.org/10.4315/0362-028x-49.9.718.

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Swiss cheese made from fully pasteurized milk developed white spots during hot room stay. This cheese was bitter and eye development was generally retarded. Streptococcus faecalis subsp. liquefaciens was isolated in high numbers from the spot; it caused bitterness in milk cultures with complete dissolution of the milk clot. The isolate was inhibitory to propionibacteria and Lactobacillus fermentum; CO2 production by Propionibacterium was depressed in broth culture in the presence of the S. faecalis subsp. liquefaciens isolate.

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Dioguardi, Mario, Mario Alovisi, Vito Crincoli, Riccardo Aiuto, Giancarlo Malagnino, Cristian Quarta, Enrica Laneve et al. "Prevalence of the Genus Propionibacterium in Primary and Persistent Endodontic Lesions: A Systematic Review". Journal of Clinical Medicine 9, n. 3 (9 marzo 2020): 739. http://dx.doi.org/10.3390/jcm9030739.

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Propionibacterium are anaerobic/aero-tolerant rod Gram-positive bacteria, and numerous studies are associated with primary and secondary endodontic infections. The data in the literature on the prevalence of Propionibacterium are conflicting, and there are studies that report conflicting data on the prevalence in primary and secondary endodontic infections. This review aims to clarify the prevalence of bacteria of the genus Propionibacterium in endodontic lesions. The present systematic review work was performed on the basis of the Prisma protocol. A search was carried out on the PubMed and Scopus databases with the use of keywords. The research produced 410 records, which, after the elimination of the overlaps and the application of the inclusion and exclusion criteria, led to a number of 36 included articles divided by the three outcomes. The first outcome concerns prevalence of bacteria of the genus Propionibacterium in primary and secondary endodontic lesions. The secondary outcome, differences in the prevalence of bacteria of the genus Propionibacterium between primary endodontic infections and secondary endodontic infections. The tertiary outcome, differences in the prevalence of Propionibacterium Acnes compared to Propionibacterium propionicum in endodontic infections. The results of the meta-analysis show that the genus Propionibacterium bacteria are more prevalent in secondary endodontic infections and that P. acnes has a higher prevalence than P. propionicum.

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Seshabhattar, Praveen, Allen Thomas Griffin, Tricia Lee e Forest Wayne Arnold. "Propionibacterium acnes Pericarditis". Infectious Diseases in Clinical Practice 20, n. 2 (marzo 2012): 119–21. http://dx.doi.org/10.1097/ipc.0b013e31823c4832.

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Winward, Kirk E., Stephen C. Pflugfelder, Harry W. Flynn, Thomas J. Roussel e Janet L. Davis. "Postoperative Propionibacterium Endophdiahnitis". Ophthalmology 100, n. 4 (aprile 1993): 447–51. http://dx.doi.org/10.1016/s0161-6420(93)31624-6.

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Zaidman, Gerald W. "Propionibacterium acnes Keratitis". American Journal of Ophthalmology 113, n. 5 (maggio 1992): 596–98. http://dx.doi.org/10.1016/s0002-9394(14)74743-9.

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Vafidis, G. "Propionibacterium acnes endophthalmitis." British Journal of Ophthalmology 75, n. 12 (1 dicembre 1991): 706. http://dx.doi.org/10.1136/bjo.75.12.706.

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Lucena-Padrós, Helena, Juan M. González, Belén Caballero-Guerrero, José Luis Ruiz-Barba e Antonio Maldonado-Barragán. "Propionibacterium olivae sp. nov. and Propionibacterium damnosum sp. nov., isolated from spoiled packaged Spanish-style green olives". International Journal of Systematic and Evolutionary Microbiology 64, Pt_9 (1 settembre 2014): 2980–85. http://dx.doi.org/10.1099/ijs.0.063032-0.

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Five strains of Gram-stain-positive bacteria were isolated from anomalous fermentations occurring in post-packaging of sealed airtight food-grade plastic pouches of Spanish-style green olives. These isolates could be grouped into two sets, which showed a similarity in their respective 16S rRNA gene sequences of 98.40 and 98.44 % with Propionibacterium acidipropionici NCFB 563 and 98.33 and 98.11 % with Propionibacterium microaerophilum M5T, respectively, and a similarity of 99.41 % between them. The 16S rRNA gene phylogeny revealed that the isolates grouped into two statistically well-supported clusters separate from P. acidipropionici NCFB 563 and P. microaerophilum M5T. Enzymic activity profiles as well as fermentation patterns differentiated these two novel bacteria from other members of the genus Propionibacterium . Finally, phenotypic, genotypic and phylogenetic data, supported the proposal of two novel species of the genus Propionibacterium , for which the names Propionibacterium olivae sp. nov. (type strain, IGBL1T = CECT 8061T = DSM 25436T) and Propionibacterium damnosum sp. nov. (type strain, IGBL13T = CECT 8062T = DSM 25450T) are proposed.

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Martínez, E. Argañaraz, J. D. Babot, M. J. Lorenzo-Pisarello, M. C. Apella e A. Perez Chaia. "Feed supplementation with avian Propionibacterium acidipropionici contributes to mucosa development in early stages of rearing broiler chickens". Beneficial Microbes 7, n. 5 (30 novembre 2016): 687–98. http://dx.doi.org/10.3920/bm2016.0077.

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Different studies in animal rearing claim the probiotic potential of species of the genus Propionibacterium. The effects of strains of Propionibacterium acidipropionici isolated from poultry intestine on microbiota activity and intestinal mucosa development were investigated in the early stage of rearing chicks and the safety of the dose used was investigated. The strains P. acidipropionici LET105 and LET107, administered as monoculture to chicks from the 1st to 14th day of life in a daily dose of 106 cfu/ml administered in the drinking water resulted harmless. The animals arrived at the expected weight for age and no differences were observed with respect to the food intake and water consumption related to control without bacteria administration. The analysis of microbiota composition revealed the presence of propionibacteria at the middle and end of the trial only in treated groups. Normal development of lactic acid bacteria and bifidobacteria, and slow colonisation by Bacteroides at the 7th day of the study was observed in the same groups. Analysis of the organic acids concentrations in the caecal content of birds revealed higher lactic acid and lower butyric acid production. Lower short chain fatty acids total concentration than expected during treatment was related to a better development of the gut mucosa. Increase in length of villus-crypt units, goblet cells counts and neutral mucins production were evidenced. Higher mucus secretion produced by dietary supplementation with propionibacteria could provide increased protection against pathogens.

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Lestari, Suryana Ayu, e Meldawati Meldawati. "TEST THE EFFECTIVENESS OF SENGGANI LEAF EKSTRACT (Melastoma candidum D.Don) AGAINST Propionibacterium acnes". Jambura Journal of Health Sciences and Research 4, n. 3 (31 maggio 2022): 709–17. http://dx.doi.org/10.35971/jjhsr.v4i3.13830.

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Senggani (Melastoma candidum D.Don) merupakan tanaman liar yang mengandung beberapa senyawa aktif yang dapat dimanfaatkan sebagai antibakteri. Kebaruan dalam penelitian ini adalah melakukan uji efektivitas ekstrak daun senggani (melastoma candidum d.don) terhadap propionibacterium acnes. Tujuan penelitian ini untuk mengetahui daya hambat ekstrak daun senggani terhadap bakteri Propionibacterium acnes. Ekstrasi dilakukan dengan cara maserasi dengan menggunakan pelarut etanol 96%. Jenis penelitian ini merupakan penelitian eksperimental dengan metode difusi cakram. Perlakuan diulang sebanyak 4 kali dengan konsentrasi 20%, 40%, 60%, 80%, dan 100%, sebagai kontrol positip digunakan clindamycin dan DMSO (Dimethyl sulfoxide) sebagai kontrol negatif. Hasil skrining fitokimia menunjukkan bahwa daun senggani mengandung senyawa, saponin, flavonoid, fenol, tannin, steroid, dan terpenoid. Hasil yang diketahui bahwa ekstrak daun senggani memiliki kemampuan menghambat pertumbuhan bakteri Propionibacterium acnes terlihat dengan adanya zona hambat yang terbentuk. Konsentrasi yang paling efektif menghambat pertumbuhan Propionibacterium acnes yaitu konsentrasi 100% sebesar 16,37 mm. Daun senggani memiliki daya hambat dalam menghambat pertumbuhan bakteri Propionibacterium acnes. Konsentrasi yang paling efektif adalah konsentrasi 100% sebesar 16,37 mm.Kata kunci: Melastoma candidum D.Don; Propionibacterium acnes; antibakteri; ekstrak ABSTRACTSenggani (Melastoma candidum D.Don) is a wild plant that contains several active compounds that can be used as antibacterial. The novelty in this research is to test the effectiveness of senggani leaf extract (Melastoma candidum d.don) against Propionibacterium acnes. The purpose of this study was to determine the inhibition of senggani leaf extract against Propionibacterium acnes bacteria. Extraction is done by maceration using 96% ethanol solvent. This type of research is an experimental study with disk diffusion method. The treatment is repeated 4 times with concentration 20%, 40%, 60%, 80%, and 100%, as a positive control used clindamycin and DMSO (Dimethyl sulfoxide) as a negative control. Phytochemical screening results showed that senggani leaves contain compounds, saponins, flavonoids, phenols, tannins, steroids, and terpenoids. The results are known that senggani leaf extract has the ability to inhibit the growth of Propionibacterium acnes bacteria seen in the presence of inhibitory zones formed. The most effective concentration to inhibit the growth of Propionibacterium acnes is 100% concentration of 16.37 mm. Senggani leaves have inhibitory power in inhibiting the growth of Propionibacterium acnes bacteria. The most effective concentration is a 100% concentration of 16.37 mm.

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Riferty, Feresta. "UJI AKTIVITAS ANTIBAKTERI EKSTRAK DAN FRAKSI BIJI PARE (Momordica charantia L.) TERHADAP BAKTERI Propionibacterium acnes". Jurnal Ilmiah Farmasi Farmasyifa 1, n. 2 (28 dicembre 2018): 119–25. http://dx.doi.org/10.29313/jiff.v1i2.3139.

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ABSTRACTBitter gourd (Momordica charantia L.) seeds is known to have efficacy in treating skin diseases, one of which is acne. This study aimed to determine the potency of antibacterial activity, minimum inhibitory concentration (MIC) and equality with comparator. The extraction process was carried out by maceration method using ethanol 96%, then fractionation was performed by using liquid-liquid extraction. The antibacterial activity of extract and fractions of bitter gourd seed against Propionibacterium acnes were done by agar diffusion method. The results showed that the extract and other fractions of bitter gourd seeds gave antibacterial activity against Propionibacterium acnes. The extract and three fractions of bitter gourd seeds inhibited the growth of Propionibacterium acnes at concentrations of 40%, 50% and 60%. The value of MIC of the extract was 30% with an 8,9 mm inhibit zone. At concentration of 40%, the fraction of ethyl acetate of bitter gourd seeds gave the highest activity in inhibiting Propionibacterium acnes compared to extract and other fractions. When it is compared with clindamycin as comparison, 1 mg of bitter gourd seed extract is equivalent to 0,80 μg clindamycin. Keywords: Bitter gourd seed, Momordica charantia L., Antibacterial, Propionibacterium acnesABSTRAK Biji pare (Momordica charantia L.) diketahui memiliki khasiat dalam mengobati penyakit kulit, salah satunya jerawat. Penelitian ini bertujuan untuk menentukan potensi aktivitas antibakteri, konsentrasi hambat minimum (KHM) dan kesetaraan dengan pembanding. Proses ekstraksi dilakukan dengan cara maserasi menggunakan pelarut etanol 96%, dilanjutkan dengan fraksinasi menggunakan ekstraksi cair-cair. Aktivitas antibakteri ekstrak dan fraksi biji pare terhadap Propionibacterium acnes dilakukan dengan metode difusi agar. Hasil penelitian menunjukkan bahwa ekstrak dan fraksi biji pare memiliki aktivitas antibakteri terhadap Propionibacterium acnes. Ekstrak dan ketiga fraksi biji pare menghambat pertumbuhan Propionibacterium acnes pada konsentrasi 40%, 50% dan 60%. Nilai KHM dari ekstrak diperoleh 30% dengan zona hambat 8,9 mm. Pada konsentrasi 40%, fraksi etil asetat biji pare memberikan aktivitas paling tinggi dalam menghambat Propionibacterium acnes dibandingkan ekstrak serta fraksi lainnya. Dibandingkan dengan klindamisin, 1mg ekstrak biji pare setara dengan 0,80 µg klindamisin. Kata Kunci: Biji pare, Momordica charantia L., antibakteri, Propionibacterium acnes

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Setyaningsih, Riska, Rani Prabandari e Dina Febrina. "Formulasi Dan Evaluasi Salep Ekstrak Etanol Bunga Kecombrang (Etlingera elatior (Jack) R.M.Sm.) Pada Penghambatan Propionibacterium acnes". Pharmacy Genius 1, n. 1 (20 ottobre 2022): 1–11. http://dx.doi.org/10.56359/pharmgen.v1i01.143.

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ABSTRAK Pendahuluan: Ekstrak bunga kecombrang memiliki kandungan flavonoid yang berperan sebagai antibakteri. Flavonoid memiliki tingkat kepolaran yang tinggi sehingga dengan mudah menembus dinding sel dari bakteri Propionibacterium acnes. Ekstrak etanol bunga kecombrang di formulasikan dalam bentuk sediaan salep untuk mempermudah pemakaian. Dasar salep yang digunakan yaitu basis larut air dengan kombinasi PEG 400 dan PEG 4000. Tujuan: Untuk mengetahui hasil evaluasi fisik sediaan salep ekstrak etanol bunga kecombrang dan untuk mengetahui daya hambat salep ekstrak etanol bunga kecombrang terhadap bakteri Propionibacterium acnes. Metode: Metode eksperimental laboratorium dimulai dengan penyiapan bahan, determinasi tanaman, pembuatan simplisia, ekstraksi, pembuatan sediaan, evaluasi fisik dan uji antibakteri. Hasil: Hasil evaluasi salep ekstrak etanol bunga kecombrang (Etlingera elatior (Jack) R.M.Sm.) berdasarkan One Way Anova pada nilai pH memiliki perbedaan yang signifikan dengan p-value > 0,05, sedangkan pada nilai daya lekat, daya sebar dan viskositas tidak memiliki perbedaan yang signifikan dengan p-value > 0,05. Berdasarkan uji Paired T test pada nilai pH, daya sebar, daya lekat dan viskositas tidak terdapat perbedaan yang signifikan antara hari ke-0 dan hari ke-13. Pada pengujian daya hambat bakteri Propionibacteriun acnes diperoleh hasil pada formula 1 sebesar 4,36 mm, formula 2 sebesar 7,95 mm dan formula 3 sebesar 9,37 mm. Kesimpulan: Salep ekstrak etanol bunga kecombrang pada formula 3 tidak masuk dalam rentang pH yang baik untuk kulit, dan pada daya sebar dari ketiga formula tidak memenuhi syarat dari daya sebar yang baik untuk kulit. Salep ekstrak etanol bunga kecombrang pada bakteri Propionibacterium acnes dengan zona hambat pada formula 1 termasuk dalam kategori lemah, formula 2 dan formula 3 termasuk dalam kategori sedang.

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Cheong, J. P. E., J. P. E. Cheong, J. D. Brooker e J. D. Brooker. "Isolation of a virulent bacteriophage from a Propionibacterium species in the sheep rumen". Australian Journal of Agricultural Research 51, n. 1 (2000): 119. http://dx.doi.org/10.1071/ar99069.

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Propionibacterium is a facultative anaerobe associated with the rumen epithelium, the presence of which may influence the anaerobic environment through oxygen scavenging, as well as providing a source of propionate. Factors such as bacteriophages that influence Propionibacterium populations may therefore be important regulators of rumen function. This study describes the isolation and identification of a ruminal Propionibacterium bacteriophage. Sheep rumen fluid was screened for Propionibacterium species and 3 isolates were identified and characterised. One isolate, PA1, was used as an indicator strain to screen for the presence of Propionibacterium-specific virulent bacteriophages. A virulent bacteriophage, PB2, was isolated from clear plaques on a lawn of PA1 cells and was shown by transmission electron microscopy to be a siphovirus-like particle comprising an icosahedral head 50 nm in diameter and a tail 140 nm in length. The bacteriophage was visibly attached to and within PA1 cells, and was shown to infect all 3 ruminal isolates of Propionibacterium and 4 of 6 clinical isolates of P. acnes. Restriction mapping of bacteriophage PB2 demonstrated a 30.8 kb genome.

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31

Sarwendah, Sarwendah, Yusliana Yusliana, Heronimus Candra G Laia, Pieter Julius Daely e Linda Chiuman. "UJI DAYA HAMBAT ANTIBAKTERI AIR PERASAN DAGING BUAH NANAS (ANANAS COMOSUS (L) MERR VAR. QUEEN) TERHADAP BAKTERI PROPIONIBACTERIUM ACNES". Jurnal Biologi Tropis 20, n. 1 (3 marzo 2020): 87. http://dx.doi.org/10.29303/jbt.v20i1.1055.

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Abstrak: Buah nanas (Ananas comosus (L) Merr) merupakan salah satu jenis buah-buahan tropis yang banyak diminati masyarakat. Buah nanas biasa dingunakan masyarakat sebagai antibakteri, antiinflamasi, antikoagulan, dan antikanker. Tujuan dari penelitian ini untuk mengetahui uji daya hambat anti bakteri air perasan daging buah nanas (Ananas comosus (L) Merr Var. Queen) terhadap bakteri Propionibacterium acnes. Penelitian ini merupakan penelitian eksperimental laboratorium dengan menggunakan metode difusi kertas cakram. Hasil dari penelitian menunjukkan bahwa air perasan daging buah nanas (Ananas comosus (L) Merr Var. Queen) pada konsentrasi 25%, 50%, 75%, 100% memiliki efektivitas sedang sebagai antibakteri dalam menghambat pertumbuhan bakteri Propionibacterium acnes, pada kontrol positif yang menggunakan klindamisin dengan rata-rata diameter zona hambat sebesar 25,8 mm memiliki pengaruh antibakteri yang sangat kuat dan untuk kontrol negatif dengan menggunakan aquades tidak menunjukkan zona hambat 0 mm pada bakteri Propionibacterium acnes.Kata kunci: Buah nanas, propionibacterium acnes, daya hambat antibakteri.Abstract: Pineapple (Ananas comosus (L) Merr) is one of the most popular fruit among people, Pineapple is used as antibacterial, anti-inlfammatory, anticoagulant and anticancer. The purpose of this study is to determine the antibacterial inhibitory test of pineapple juice (Ananas comosus (L) Merr Var. Queen) against Propionibacterium acnes. This research is a laboratory experimental using paper disc diffisuion method. The results of this study indicate that pineapple juice at concentration of 25%, 50%, 75%, 100% it has a moderately antibacterial effectiveness in inhibit the growth of Propionibacterium acnes, positive control using clindamycin with an average inhibition zone diameter of 25.8 mm has a very strong antibacterial effect and for negative control using aquades does not show 0 mm inhibition zone in Propionibacterium acnes bacteria.Keywords: Pineapple fruit, propionibacterium acnes, antibacterial inhibition.

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Fernandez Sampedro, Marta, Kerryl E. Piper, Andrew McDowell, Sheila Patrick, Jayawant N. Mandrekar, Mark S. Rouse, James M. Steckelberg e Robin Patel. "Species of Propionibacterium and Propionibacterium acnes phylotypes associated with orthopedic implants". Diagnostic Microbiology and Infectious Disease 64, n. 2 (giugno 2009): 138–45. http://dx.doi.org/10.1016/j.diagmicrobio.2009.01.024.

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33

Indarto, Indarto, Windy Narulita, Bambang Sri Anggoro e Aulia Novitasari. "Aktivitas Antibakteri Ekstrak Daun Binahong Terhadap Propionibacterium Acnes". Biosfer: Jurnal Tadris Biologi 10, n. 1 (30 giugno 2019): 67–78. http://dx.doi.org/10.24042/biosfer.v10i1.4102.

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Tujuan penelitian ini adalah untuk mengetahui apakah ekstrak daun binahong dapat menghambat pertumbuhan bakteri Propionibacterium acnes. Penelitian ini merupakan penelitian eksperimental laboratorium dengan menggunakan Rancangan Acak Lengkap (RAL) dengan lima perlakuan. Perlakuan diulang sebanyak 3 kali dengan berbagai macam konsentrasi dari 20%, 40%, 60%, 80%, dan 100%, serta klindamisin sebagai pembanding dan aquades sebagai kontrol. Ektrak daun binahong diperoleh dari maserasi daun binahong dengan pelarut metanol dan dipartisi dengan menggunakan pelarut etil asetat, kemudian dilakukan uji aktivitas antibakteri terhadap bakteri Propionibacterium acnes. Parameter yang diamati adalah diameter zona hambat pertumbuhan bakteri Propionibacterium acnes. Analisis data menggunakan ANOVA (Analysis of Variance). Hasilnya diketahui bahwa ekstrak daun binahong memiliki kemampuan menghambat pertumbuhan bakteri Propionibacterium acnes terlihat dengan adanya zona hambat yang terbentuk. Konsentrasi yang paling efektif menghambat pertumbuhan bakteri Propionibacterium acnes adalah pada konsentrasi 100% sebesar 9,00 mm pada waktu 24 jam dan 11,20 mm pada waktu 48 jam.

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McDowell, Andrew, Emma Barnard, Jared Liu, Huiying Li e Sheila Patrick. "Proposal to reclassify Propionibacterium acnes type I as Propionibacterium acnes subsp. acnes subsp. nov. and Propionibacterium acnes type II as Propionibacterium acnes subsp. defendens subsp. nov." International Journal of Systematic and Evolutionary Microbiology 66, n. 12 (1 dicembre 2016): 5358–65. http://dx.doi.org/10.1099/ijsem.0.001521.

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35

Kiatpapan, Pornpimon, Yoshiteru Hashimoto, Hisako Nakamura, Yong-Zhe Piao, Hisayo Ono, Mitsuo Yamashita e Yoshikatsu Murooka. "Characterization of pRGO1, a Plasmid from Propionibacterium acidipropionici, and Its Use for Development of a Host-Vector System in Propionibacteria". Applied and Environmental Microbiology 66, n. 11 (1 novembre 2000): 4688–95. http://dx.doi.org/10.1128/aem.66.11.4688-4695.2000.

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ABSTRACT The complete nucleotide sequence of pRGO1, a cryptic plasmid fromPropionibacterium acidipropionici E214, was determined. pRGO1 is 6,868 bp long, and its G+C content is 65.0%. Frame analysis of the sequence revealed six open reading frames, which were designated Orf1 to Orf6. The deduced amino acid sequences of Orf1 and Orf2 showed extensive similarities to an initiator of plasmid replication, the Rep protein, of various plasmids of gram-positive bacteria. The amino acid sequence of the putative translation product of orf3 exhibited a high degree of similarity to the amino acid sequences of DNA invertase in several bacteria. For the putative translation products of orf4,orf5, and orf6, on the other hand, no homologous sequences were found. The function of these open reading frames was studied by deletion analysis. A shuttle vector, pPK705, was constructed for shuttling between Escherichia coli and a Propionibacterium strain containingorf1 (repA), orf2(repB), orf5, and orf6 from pRGO1, pUC18, and the hygromycin B-resistant gene as a drug marker. Shuttle vector pPK705 successfully transformed Propionibacterium freudenreichii subsp. shermanii IFO12426 by electroporation at an efficiency of 8 × 106 CFU/μg of DNA under optimized conditions. Transformation of various species of propionibacteria with pPK705 was also performed at efficiencies of about 104 to 107 CFU/μg of DNA. The vector was stably maintained in strains of P. freudenreichiisubsp. shermanii, P. freudenreichii, P. pentosaceum, and P. freudenreichii subsp.freudenreichii grown under nonselective conditions. Successful manipulation of a host-vector system in propionibacteria should facilitate genetic studies and lead to creation of genes that are useful industrially.

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Lister, I. Nyoman Ehrich. "PERBANDINGAN UJI EFEKTIVITAS EKSTRAK BENGKUANG (PACHYRIZUS AROSUS) DAN DAUN SIRIH HIJAU (PIPER BETLE L.) TERHADAP BAKTERI PROPIONIBACTERIUM ACNES". Jurnal Keperawatan Priority 4, n. 1 (2 gennaio 2021): 60–68. http://dx.doi.org/10.34012/jukep.v4i1.1434.

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Propionibacterium acnes is a gram-positive bacteria and consists of the Propionibacteriaceae family. Propionibacterium acnes is a bacteria that causes acne or acne vulgaris, a disease that is quite disturbing and gets the attention that occurs in adolescents and young adults. This research aimed to compare and determine the effectiveness of the extract of yam (Pachyrizus arosus) and green betel leaf (Piper betle L.) in inhibiting the growth of Propionibacterium acnes bacteria. The research variables were concentrations of yam extract (Pachyrizus arosus) 5%, 10%, 15%, 20% and green betel leaf (Piper betle L.) 1,5%, 3%, 5%, 7,5%. The results showed that there was an inhibiting zone against the growth of Propionibacterium acnes bacteria.

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McDowell, Andrew, Emma Barnard, Jared Liu, Huiying Li e Sheila Patrick. "Corrigendum: Proposal to reclassify Propionibacterium acnes type I as Propionibacterium acnes subsp. acnes subsp. nov. and Propionibacterium acnes type II as Propionibacterium acnes subsp. defendens subsp. nov." International Journal of Systematic and Evolutionary Microbiology 67, n. 11 (1 novembre 2017): 4880. http://dx.doi.org/10.1099/ijsem.0.002385.

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Marliana, Marliana, Sartini Sartini e Abdul Karim. "EFEKTIVITAS BEBERAPA PRODUK PEMBERSIH WAJAH ANTIACNE TERHADAP BAKTERI PENYEBAB JERAWAT Propionibacterium acnes". BIOLINK (Jurnal Biologi Lingkungan, Industri, Kesehatan) 5, n. 1 (8 agosto 2018): 31. http://dx.doi.org/10.31289/biolink.v5i1.1668.

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<em>The purpose of this study was to determine the effectiveness of some antiacne facial cleanser products against bacteria that cause acne Propionibacterium acnes, which conducted experimentally using 6 product against of antiacne facial cleanser bacteria cause Propionibacterium acnes with three replication. The parameters observed were the inhibitory zone diameters. The results showed that of some antiacne facial cleanser products that are most effective in inhibiting the growth of acne-causing bacteria Propionibacterium acnes is the product CCA code, with a 35 mm inhibit zone, because CCA products were contain 6 combinations of natural ingredients from Hamamelis virginia, Melaleuca alternifolia, Cinnamomum zeylanicum, Cedrus atlantica, Portulaca oleracea and olive oil All antiacne products are shown to have effectiveness against the bacteria Propionibacterium acnes.</em>

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Silvia, Eka, Ringgo Alfarisi, Arief Effendi e Muhammad Alva Rizqy. "Efektifitas Antibiotik Azelaic Acid Terhadap Propioni-Baktterium Acne Dengan Metode Difusi Pada Pasien Acne Vulgaris". MAHESA : Malahayati Health Student Journal 2, n. 3 (15 maggio 2022): 586–97. http://dx.doi.org/10.33024/mahesa.v2i3.6428.

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ABSTRACT Acne vulgaris is a form of chronic inflammation of the hair follicles of the sebaceous glands in the form of multifactorial and clinical manifestations of acne, papules, pustules, lymph nodes, and cysts. Propionibacterium acnes is a bacterium involved in acne inflammation and Azelaic acid is one of the acne vulgaris treatments that have antimicrobial and anti-inflammatory effects. Know the degree of effectiveness of Azelaic Acid antibiotic against propionibacterium acne by in vitro diffusion method and compare it with several antibiotics (clindamycin, erythromycin, benzoyl peroxide, tetracycline) and Lactobacillus acidophilus. The study tested the effectiveness of Azelaic acid antibiotics against propionibacterium acne by in vitro diffusion method and analyzed the data using Shapiro-Wilk for data normality. After being declared to be normally distributed (p>0.05), an unpaired T-test was performed. If the data were not normally distributed, the Mann-Whitney test was performed to test the comparison of 1 and 2. Results: The average effectiveness of the antibiotics Azelaic acid is 33,589 mm and Vaseline 0 mm against Propionibacterium acnes in vitro. Based on the unpaired T-Test, Sig. (2-Tailed) = 0.000, which means that there is a significant difference in each variable. Conclusion: There is a difference in the effectiveness of Azelaic acid with Vaseline on the growth of Propionibacterium acnes by diffusion method in vitro and ranks third highest, including a very strong inhibition zone Keywords: Acne Vulgaris, Azelaic Acid, Propionibacterium Acnes ABSTRAK Acne vulgaris adalah suatu bentuk peradangan kronis pada folikel rambut kelenjar sebaceous berupa multifaktor dan manifestasi klinis berupa jerawat, papula, pustula, kelenjar getah bening dan kista. Propionibacterium acnes merupakan bakteri yang terlibat dalam peradangan jerawat dan Azelaic acid salah satu pengobatan akne vulgaris yang memiliki efek antimikroba dan anti-inflamasi. Tujuan penelitian ini untuk mengetahui derajat keefektivitasan antibiotik Azelaic Acid terhadap propionibacterium acne dengan metode difusi secara in vitro dan membandingkan dengan beberapa antibiotik (klindamisin, eritromisin, benzoil peroksida, tetrasiklin) serta Lactobacillus achidopilus. Penelitian uji efektifitas antibiotik Azelaic acid terhadap propinibacterium acnes dengan metode difusi secara in vitro dan menganalisis data menggunakan Shapiro-wilk untuk normalitas data. Setelah dinyatakan berdistribusi normal (p>0,05) maka dilakukan uji T-Tidak berpasangan jika data tidak berdistribusi normal maka dilakukan uji MannWhitney untuk menguji perbandingan 1 dan 2. Didapatkan rata-rata efektifias antibiotik Azelaic acid 33,589 mm dan Vaseline 0 mm terhadap Propionibacterium acnes secara in vitro. Berdasarkan uji T-Test tidak berpasangan di peroleh Sig.(2-Tailed) = 0,000 yang berarti terdapat perbedaan yang bermakna pada setiap variabel. Terdapat perbedaan efektivitas antibiotik Azelaic acid dengan vaseline terhadap pertumbuhan Propionibacterium acnes dengan metode difusi secara in vitro dan menempati urutan ke ketiga tertinggi, termasuk zona hambat sangat kuat. Kata Kunci: Acne Vulgaris, Azelaic Acid, Propionibacterium Acnes

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Nita, Cici Nasya, Rosha Kurnia Fembriyanto e Nur Annis Hidayati. "POTENSI DAUN KAYU LUBANG (Timonius flavescens (Jacq.) Baker) SEBAGAI ALTERNATIF MENGATASI JERAWAT". EKOTONIA: Jurnal Penelitian Biologi, Botani, Zoologi dan Mikrobiologi 3, n. 2 (18 gennaio 2019): 50–54. http://dx.doi.org/10.33019/ekotonia.v3i2.759.

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Kayu lubang are plants which one traditionally used to acne treatment. Scientifically acne is caused bacteria’s such as Propionibacterium acnes dan Staphylococcus aureus infection. The growth of Propionibacterium acnes and Staphylococcus aureus be inhibition with phytochemical compounds contained leaves of kayu lubang. The purpose of this research was to identification phytochemical compunds from rude extract ethanol leaves of kayu lubang and which consentration extract that shows the optimum to inhibition growth of Propionibacterium acnes dan Staphylococcus aureus in antibacterial activity test. Phytochemical test conducted qualitative and antibacterial activity test using disc diffusion with concentration 15%, 20%, 25%, 30%, 35% dan 40% in 3 replication. Based on the results showed that in general those leaves contain alcaloid, phenol, tannin and steroid. The optimum inhibition zone results of extract inhibition bacterial growth at concentration of 40%, inhibition zone Propionibacterium acnes inhibition zone of 6,83 mm while Staphylococcus aureus bacteria was 5,53 mm. Based on the results of statistical tests DMRT effects of extracts leaves of kayu lubang against the bacteria Propionibacterium acnes and Staphylococcus aureus showed significant differences among for 95% confidence.

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Hsu, Jason E., Roger E. Bumgarner e Frederick A. Matsen. "Propionibacterium in Shoulder Arthroplasty". Journal of Bone and Joint Surgery 98, n. 7 (aprile 2016): 597–606. http://dx.doi.org/10.2106/jbjs.15.00568.

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Rehberger, T. G., e B. A. Glatz. "Characterization of Propionibacterium plasmids." Applied and Environmental Microbiology 56, n. 4 (1990): 864–71. http://dx.doi.org/10.1128/aem.56.4.864-871.1990.

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Teichmann, Klaus D. "Treatment of Propionibacterium Endophthalmitis". Ophthalmology 100, n. 11 (novembre 1993): 1600–1601. http://dx.doi.org/10.1016/s0161-6420(93)31406-5.

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44

Kowalski, Todd J., Elie F. Berbari, Paul M. Huddleston, James M. Steckelberg e Douglas R. Osmon. "Propionibacterium acnes Vertebral Osteomyelitis". Clinical Orthopaedics and Related Research 461 (agosto 2007): 25–30. http://dx.doi.org/10.1097/blo.0b013e318073c25d.

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Martín Gonzálvez, J. A., P. Melero Almau e A. Merino Múgica. "Empiema por Propionibacterium acnes". Archivos de Bronconeumología 33, n. 2 (febbraio 1997): 107. http://dx.doi.org/10.1016/s0300-2896(15)30665-7.

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Corne, Ph, E. Gal, S. Yeche, D. Reynaud e A. Dubois. "Endocardite à Propionibacterium acnes". Médecine et Maladies Infectieuses 27, n. 12 (dicembre 1997): 1039–40. http://dx.doi.org/10.1016/s0399-077x(97)80238-7.

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Chanet, Valérie, Jean-Pierre Romaszko, Jean-Marc Rolain e Jean Beytout. "Adénite à Propionibacterium acnes". La Presse Médicale 34, n. 14 (agosto 2005): 1005–6. http://dx.doi.org/10.1016/s0755-4982(05)84100-4.

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48

Garny de La Rivière, C., A. Dadban, G. Chaby e C. Lok. "Endocardite à Propionibacterium acnes". Annales de Dermatologie et de Vénéréologie 140, n. 12 (dicembre 2013): S591—S592. http://dx.doi.org/10.1016/j.annder.2013.09.519.

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Bojar, Richard A., e Keith T. Holland. "Acne and propionibacterium acnes". Clinics in Dermatology 22, n. 5 (settembre 2004): 375–79. http://dx.doi.org/10.1016/j.clindermatol.2004.03.005.

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50

Coden, Daniel J. "Propionibacterium acnes Orbital Abscess". Archives of Ophthalmology 108, n. 4 (1 aprile 1990): 481. http://dx.doi.org/10.1001/archopht.1990.01070060029012.

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