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Letteratura scientifica selezionata sul tema "Plant proteins Genetic aspects"

Autore: Grafiati

Pubblicato: 4 giugno 2021

Ultima modifica: 14 febbraio 2022

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Articoli di riviste sul tema "Plant proteins Genetic aspects"

1

Pandey, Sona. "Plant receptor-like kinase signaling through heterotrimeric G-proteins". Journal of Experimental Botany 71, n. 5 (13 gennaio 2020): 1742–51. http://dx.doi.org/10.1093/jxb/eraa016.

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Abstract Heterotrimeric G-proteins regulate multiple aspects of plant growth, development, and response to biotic and abiotic stresses. While the core components of heterotrimeric G-proteins and their basic biochemistry are similar in plants and metazoans, key differences exist in their regulatory mechanisms. In particular, the activation mechanisms of plant G-proteins appear diverse and may include both canonical and novel modes. Classical G-protein-coupled receptor-like proteins exist in plants and interact with Gα proteins, but their ability to activate Gα by facilitating GDP to GTP exchange has not been demonstrated. Conversely, there is genetic and functional evidence that plant G-proteins interact with the highly prevalent receptor-like kinases (RLKs) and are phosphorylated by them. This suggests the exciting scenario that in plants the G-proteins integrate RLK-dependent signal perception at the plasma membrane with downstream effectors. Because RLKs are active kinases, it is also likely that the activity of plant G-proteins is regulated via phosphorylation/dephosphorylation rather than GTP–GDP exchange as in metazoans. This review discusses our current knowledge of the possible RLK-dependent regulatory mechanisms of plant G-protein signaling in the context of several biological systems and outlines the diversity that might exist in such regulation.

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2

Tichá, Tereza, Despina Samakovli, Anna Kuchařová, Tereza Vavrdová e Jozef Šamaj. "Multifaceted roles of HEAT SHOCK PROTEIN 90 molecular chaperones in plant development". Journal of Experimental Botany 71, n. 14 (7 aprile 2020): 3966–85. http://dx.doi.org/10.1093/jxb/eraa177.

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Abstract HEAT SHOCK PROTEINS 90 (HSP90s) are molecular chaperones that mediate correct folding and stability of many client proteins. These chaperones act as master molecular hubs involved in multiple aspects of cellular and developmental signalling in diverse organisms. Moreover, environmental and genetic perturbations affect both HSP90s and their clients, leading to alterations of molecular networks determining respectively plant phenotypes and genotypes and contributing to a broad phenotypic plasticity. Although HSP90 interaction networks affecting the genetic basis of phenotypic variation and diversity have been thoroughly studied in animals, such studies are just starting to emerge in plants. Here, we summarize current knowledge and discuss HSP90 network functions in plant development and cellular homeostasis.

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3

Soyano, Takashi, Masaki Ishikawa, Ryuichi Nishihama, Satoshi Araki, Mayumi Ito, Masaki Ito e Yasunori Machida. "Control of plant cytokinesis by an NPK1–mediated mitogen–activated protein kinase cascade". Philosophical Transactions of the Royal Society of London. Series B: Biological Sciences 357, n. 1422 (29 giugno 2002): 767–75. http://dx.doi.org/10.1098/rstb.2002.1094.

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Cytokinesis is the last essential step in the distribution of genetic information to daughter cells and partition of the cytoplasm. In plant cells, various proteins have been found in the phragmoplast, which corresponds to the cytokinetic apparatus, and in the cell plate, which corresponds to a new cross wall, but our understanding of the functions of these proteins in cytokinesis remains incomplete. Reverse genetic analysis of NPK1 MAPKKK (nucleus– and phragmoplast–localized protein kinase 1 mitogen–activated protein kinase kinase kinase) and investigations of factors that might be functionally related to NPK1 have helped to clarify new aspects of the mechanisms of cytokinesis in plant cells. In this review, we summarize the evidence for the involvement of NPK1 in cytokinesis. We also describe the characteristics of a kinesin–like protein and the homologue of a mitogen–activated protein kinase that we identified recently, and we discuss possible relationships among these proteins in cytokinesis.

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4

Li, Lei, e Detlef Weigel. "One Hundred Years of Hybrid Necrosis: Hybrid Autoimmunity as a Window into the Mechanisms and Evolution of Plant–Pathogen Interactions". Annual Review of Phytopathology 59, n. 1 (25 agosto 2021): 213–37. http://dx.doi.org/10.1146/annurev-phyto-020620-114826.

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Hybrid necrosis in plants refers to a genetic autoimmunity syndrome in the progeny of interspecific or intraspecific crosses. Although the phenomenon was first documented in 1920, it has been unequivocally linked to autoimmunity only recently, with the discovery of the underlying genetic and biochemical mechanisms. The most common causal loci encode immune receptors, which are known to differ within and between species. One mechanism can be explained by the guard hypothesis, in which a guard protein, often a nucleotide-binding site–leucine-rich repeat protein, is activated by interaction with a plant protein that mimics standard guardees modified by pathogen effector proteins. Another surprising mechanism is the formation of inappropriately active immune receptor complexes. In this review, we summarize our current knowledge of hybrid necrosis and discuss how its study is not only informing the understanding of immune gene evolution but also revealing new aspects of plant immune signaling.

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5

Frolova, T. S., V. A. Cherenko, O. I. Sinitsyna e A. V. Kochetov. "Genetic aspects of potato resistance to phytophthorosis". Vavilov Journal of Genetics and Breeding 25, n. 2 (29 aprile 2021): 164–70. http://dx.doi.org/10.18699/vj21.020.

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Phytophthora infestans Mont. de Bary is the main oomycete pathogen of cultivated crops in the family Solanaceae, especially potato (Solanum tuberosum). Because potato is the fourth most cultivated crop worldwide, its annual losses from late blight are tremendous. Studies of the basic mechanisms of interaction between potato and the late blight pathogen not only expand the fundamental knowledge in this area, but also open up new possibilities for regulating these interactions in order to increase resistance to the pathogen. The interaction of potato and the late blight pathogen can be considered from a genetic point of view, and it is interesting to consider both the response of the potato to the colonization process by P. infestans and the change in gene activity in late blight during plant infection. We can also investigate this process by changing the profile of secondary metabolites of the host and the pathogen. In addition to fundamental work in this area, applied work in the form of the development of new preparations for protecting potatoes is of no less importance. This review briefly describes the main stages of studies of potato resistance to late blight, starting almost from the first works. Much attention is paid to key works on changing the profile of secondary metabolites phytoalexins. A separate section is devoted to the description of both qualitative and quantitative characteristics of potato resistance to the late blight pathogen: their contribution to overall resistance, gene mapping, and regulation capabilities. Both types of traits are important for potato breeding: quantitative resistance due to R-genes is quickly overcome by the pathogen, while quantitative trait loci make it possible to create varieties with almost absolute resistance due to the pyramid of effective genes. The latest approaches in molecular biology make it possible to study translatomic profiles, which makes it possible to look at the interaction of potatoes and the late blight pathogen at a different angle. It has been shown that the process of potato colonization affects not only the activity of various genes and the profile of secondary metabolites: proteinsmarkers of the response to infection from potatoes have also been identified: they are pathogen-bound proteins and plastid carbonic anhydrase. On the part of P. infestans, fungal cellulose synthase proteins and haustorium-specific membrane protein were markers of infection. Thus, the review contains information on the most relevant complex studies of the genetic mechanisms of potato resistance to late blight.

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6

Hadi, Joshua, e Gale Brightwell. "Safety of Alternative Proteins: Technological, Environmental and Regulatory Aspects of Cultured Meat, Plant-Based Meat, Insect Protein and Single-Cell Protein". Foods 10, n. 6 (28 maggio 2021): 1226. http://dx.doi.org/10.3390/foods10061226.

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Food security and environmental issues have become global crises that need transformative solutions. As livestock production is becoming less sustainable, alternative sources of proteins are urgently required. These include cultured meat, plant-based meat, insect protein and single-cell protein. Here, we describe the food safety aspects of these novel protein sources, in terms of their technological backgrounds, environmental impacts and the necessary regulatory framework for future mass-scale production. Briefly, cultured meat grown in fetal bovine serum-based media can be exposed to viruses or infectious prion, in addition to other safety risks associated with the use of genetic engineering. Plant-based meat may contain allergens, anti-nutrients and thermally induced carcinogens. Microbiological risks and allergens are the primary concerns associated with insect protein. Single-cell protein sources are divided into microalgae, fungi and bacteria, all of which have specific food safety risks that include toxins, allergens and high ribonucleic acid (RNA) contents. The environmental impacts of these alternative proteins can mainly be attributed to the production of growth substrates or during cultivation. Legislations related to novel food or genetic modification are the relevant regulatory framework to ensure the safety of alternative proteins. Lastly, additional studies on the food safety aspects of alternative proteins are urgently needed for providing relevant food governing authorities with sufficient data to oversee that the technological progress in this area is balanced with robust safety standards.

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7

Dziechciarková, M., A. Lebeda, I. Doležalová e D. Astley. "Characterization of Lactuca spp. germplasm by protein and molecular markers – a review". Plant, Soil and Environment 50, No. 2 (21 novembre 2011): 47–58. http://dx.doi.org/10.17221/3680-pse.

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The genus Lactuca L. belongs to one of the largest plant families, Asteraceae. Lactuca L. is represented by ca 100 species distributed in different geographical areas and ecological conditions. This is one of the reasons why this genus is characterised by very broad variation of different characters. Electrophoretic detection of some proteins (isozymes) has been applied to the study of genetic variability of Lactuca spp. individuals and populations. The development of molecular genetic methods (RFLP, Restriction Fragment Length Polymorphism; PCR methods: RAPD, Random Amplified Polymorphic DNA; AFLP, Amplified Fragment Length Polymorphism; minisatellites and microsatellites fingerprinting or SSR, Simple Sequence Repeats) and their application has contributed to the elucidation of various aspects related to the taxonomy, variability, biodiversity, genetics and breeding within the genus Lactuca L. Further potential application of these methods is discussed.

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8

Kayser, Oliver. "Ethnobotany and Medicinal Plant Biotechnology: From Tradition to Modern Aspects of Drug Development". Planta Medica 84, n. 12/13 (24 maggio 2018): 834–38. http://dx.doi.org/10.1055/a-0631-3876.

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AbstractSecondary natural products from plants are important drug leads for the development of new drug candidates for rational clinical therapy and exhibit a variety of biological activities in experimental pharmacology and serve as structural template in medicinal chemistry. The exploration of plants and discovery of natural compounds based on ethnopharmacology in combination with high sophisticated analytics is still today an important drug discovery to characterize and validate potential leads. Due to structural complexity, low abundance in biological material, and high costs in chemical synthesis, alternative ways in production like plant cell cultures, heterologous biosynthesis, and synthetic biotechnology are applied. The basis for any biotechnological process is deep knowledge in genetic regulation of pathways and protein expression with regard to todays “omics” technologies. The high number genetic techniques allowed the implementation of combinatorial biosynthesis and wide genome sequencing. Consequently, genetics allowed functional expression of biosynthetic cascades from plants and to reconstitute low-performing pathways in more productive heterologous microorganisms. Thus, de novo biosynthesis in heterologous hosts requires fundamental understanding of pathway reconstruction and multitude of genes in a foreign organism. Here, actual concepts and strategies are discussed for pathway reconstruction and genome sequencing techniques cloning tools to bridge the gap between ethnopharmaceutical drug discovery to industrial biotechnology.

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9

Sluse, Francis E., e Wieslawa Jarmuszkiewicz. "Uncoupling proteins outside the animal and plant kingdoms: functional and evolutionary aspects". FEBS Letters 510, n. 3 (6 dicembre 2001): 117–20. http://dx.doi.org/10.1016/s0014-5793(01)03229-x.

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10

Tripathy, Manas K., Renu Deswal e Sudhir K. Sopory. "Plant RABs: Role in Development and in Abiotic and Biotic Stress Responses". Current Genomics 22, n. 1 (12 aprile 2021): 26–40. http://dx.doi.org/10.2174/1389202922666210114102743.

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Endosomal trafficking plays an integral role in various eukaryotic cellular activities and is vital for higher-order functions in multicellular organisms. RAB GTPases are important proteins that influence various aspects of membrane traffic, which consequently influence many cellular functions and responses. Compared to yeast and mammals, plants have evolved a unique set of plant-specific RABs that play a significant role in their development. RABs form the largest family of small guanosine triphosphate (GTP)-binding proteins, and are divided into eight sub-families named RAB1, RAB2, RAB5, RAB6, RAB7, RAB8, RAB11 and RAB18. Recent studies on different species suggest that RAB proteins play crucial roles in intracellular trafficking and cytokinesis, in autophagy, plant microbe interactions and in biotic and abiotic stress responses. This review recaptures and summarizes the roles of RABs in plant cell functions and in enhancing plant survival under stress conditions.

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Più fonti

Tesi sul tema "Plant proteins Genetic aspects"

1

Singh, Nagendra Kumar. "The structure and genetic control of endosperm proteins in wheat and rye". Title page, contents and abstract only, 1985. http://web4.library.adelaide.edu.au/theses/09PH/09phs6174.pdf.

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2

Cheung, Wai-ying, e 張慧盈. "Characterization of plant homeodomain finger protein 11 (PHF11), a candidate tumor suppressor, in esophageal squamous cell carcinoma". Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2012. http://hub.hku.hk/bib/B50162834.

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Esophageal squamous cell carcinoma (ESCC) is a common cancer worldwide with a high mortality rate. High occurrence of ESCC is observed in Southeast Asia. Identification and characterization of ESCC important tumor suppressor genes will be highly beneficial to the understanding of the disease and for the early diagnosis and improvement of therapy for the cancer. In our previous microcell-mediated chromosome transfer (MMCT) studies, the transfer of an intact chromosome 13 into the recipient ESCC cell line revealed the tumor suppressive ability and putative tumor suppressive function of chromosome 13 in ESCC. One candidate gene, Plant-Homeodomain Finger Protein 11(PHF11), was identified from the study and selected for further functional studies in this current study. PHF11, located on chromosomal region 13q14, contains two plant homeodomain fingers and is a member of the PHD finger protein family. PHF11was reported to be associated with asthma and atopic diseases, yet no studies of PHF11havebeen reported in cancer to date. This study is the first to report the functional role of PHF11in tumor suppression. In this current study, two isoforms of PHF11, PHF11aand b, were reintroduced into ESCC cell lines by methods of transient tranfection and lentiviral-infection. In vitro studies showed both isoforms have cell proliferation and colony-formation inhibition abilities. In the nude mouse tumorigenicity assay, however, it was revealed that only thePHF11aisoform was tumor suppressive in vivo. No differences in angiogenesis-related factors and apoptosis-related factors were observed in PHF11a-and b-expressing cells. Further studies by Western blotting analysis and flow cytometry analysis showed that PHF11amay play a role in delaying cell cycle progression by the down-regulation of cyclin expression, while the PHF11bmay be functionally inactive, The results of this current study further confirm the tumor suppressive role of PHF11ain ESCC, whereas the PHF11b isoform was unable to suppress tumor formation in vivo. Further study of the PHF11 isoforms to identify their differential functions and interacting partners will provide a better understanding of the mechanism by which PHF11a suppressestumor growth.
published_or_final_version
Clinical Oncology
Master
Master of Philosophy

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3

Maree, H. J. (Hans Jacob). "The expression of Dianthin 30, a ribosome inactivating protein". Thesis, Stellenbosch : Stellenbosch University, 2003. http://hdl.handle.net/10019.1/53633.

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Thesis (MSc)--Stellenbosch University, 2003.
ENGLISH ABSTRACT: Ribosome inactivating proteins (RIPs) are currently classified as rRNA N-glycosidases, but also have polynucleotide: adenosine glycosidase activity. RIPs are believed to have anti-viral and anti-fungal properties, but the exact mechanism of these proteins still need to be elucidated.The mechanism of resistance however, appears to be independent of the pathogen. For resistance the RIP terminates virus infected plant cells and stops the reproduction and spread of the virus. Transgenic plants containing RIPs should thus be resistant to a wide range of viruses. The ultimate goal of the larger project of which this forms part is the development of virus resistant plants. To monitor the expression of a RIP in a transgenic plant a detection method had to be developed. Antibody detection of the RIP was decided upon as the most cost effective method. The RIP, Dianthin 30 from Dianthus caryophyllus (carnation), was used and expressed in bacterial and insect expression systems. The bacterial expression experiments were done using the pET expression system in BL21(DE3)pLysS cells. The expression in this system yielded recombinant protein at a very low concentration. Expression experiments were also performed in insect tissue culture with the baculovirus vector BAC-TO-BAC™.With this system the expression was also too low to be used for the production of antibodies. A Dianthin 30 specific peptide was then designed and then produced by Bio-Synthesis. This peptide was then used to raise antibodies to detect Dianthin 30. These antibodies were tested on Dianthus caryophyllus proteins. To establish if this detection method was effective to monitor the expression in plants, tobacco plants were transformed with Agrobacterium tumefaciens containing Dianthin 30 in the pART27 plant expression vector. The putative transformed plants were analysed with peR and Southern blots.
AFRIKAANSE OPSOMMING: Tans word Ribosomale-inaktiverende proteïene (RIPs) geklassifiseer as rRNA N-glikosidase wat ook polinukleotied: adenosien glikosidase aktiwiteit bevat. Daar word geglo dat RIPs anti-virale en anti-fungus eienskappe bevat, maar die meganisme van beskerming word nog nie ten volle verstaan nie. Dit is wel bewys dat die meganisme van weerstand onafhanklik is van die patogeen. Virus geinfekteerde plantselle word deur die RIP gedood om die voortplanting en verspreiding te bekamp en sodoende word weerstand bewerkstellig. Transgeniese plante wat dan 'n RIP bevat sal dus weerstandbiedend wees teen 'n wye spektrum virusse. Die hoofdoel van die breër projek, waarvan die projek deel uitmaak: is die ontwikkeling van virusbestande plante. Om die uitdrukking van die RIP in die transgeniese plante te kontroleer, moes 'n deteksie metode ontwikkel word. Die mees koste effektiewe deteksie metode is met teenliggame. Die RIP, Dianthin 30 from Dianthus caryophyllus (angelier) was gebruik vir uitdrukking in bakteriele- en insekweefselkultuur. Die bakteriele uitdrukkingseksperimente was gedoen met die pET uitdrukkings sisteem III BL21(DE3)pLysS selle. Die uitdrukking in die sisteem het slegs rekombinante proteïene gelewer in uiters lae konsentrasies. Uitdrukkingseksperimente was ook gedoen in insekweefselkultuur met die baculovirus vektor BAC-To- BACTM. Met die sisteem was die uitdrukking ook veels te laag om bruikbaar te wees vir die produksie van teenliggame. Daar is toe 'n peptied ontwerp wat Dianthin 30 kan verteenwoordig vir die produksie van teenliggame. Die teenliggame is getoets teen Dianthus caryophyllus proteïene. Om vas te stel of die deteksiemetode wel die uitdrukking van Dianthin 30 sal kan monitor, is tabak ook getransformeer met Dianthin 30. Die transformasies is gedoen met die hulp van Agrobacterium tumefaciens en die pART27 plant uitdrukkings vektor. Die plante is getoets met die polimerase ketting reaksie en Southern klad tegnieke.

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4

Joubert, Dirk Albert 1973. "Regulation of the Vitis vinifera PGIP1 gene encoding a polygalacturonase-inhibiting protein". Thesis, Stellenbosch : Stellenbosch University, 2004. http://hdl.handle.net/10019.1/53759.

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Thesis (PhD)--University of Stellenbosch, 2004.
ENGLISH ABSTRACT: Plant-pathogen interactions have been intensively investigated in the last decade. This major drive towards understanding the fundamental aspects involved in plant disease resistance is propelled by the obvious agricultural and economical benefits that are intrinsically linked to disease and stress resistant plants. It is, therefore, not surprising that fundamental research in this area is not just restricted to model organisms, such as Arabidopsis and tobacco, but also extends to more traditional crop plants, such as maize, bean, soybean, apples, grapevine etc. In grapevine for instance, several genes involved in disease resistance have been isolated. One of these genes, encoding for a polygalacturonase inhibiting protein (PGIP), has been studied extensively. PGIPs are cell wall bound, contain leucine rich repeats (LRR) and are found in all dicotyledonous plants so far examined. In most cases, pgip genes occur in small multigene families and expression is often tissue specific and developmentally regulated. Up-regulation of PGIP-encoding genes typically occurs upon pathogen infection, treatment with elicitors, salicylic acid (SA), jasmonic acid (JA), cold treatment and wounding. Differential regulation and specificity have been shown to occur between members of the same multigene family. Differential regulation even extends to the utilization of separate pathways to induce pgip genes from the same family in response to a single stress stimulus. PGIPs interact with cell wall macerating polygalacturonases (PGs) that are secreted by pathogenic fungi during the infection process. The antifungal action of PGIPs is thought to depend on a dual action. The physical interaction of PGIP with PGs has an inhibitionary effect, resulting in (i) a slower fungal infection rate and (ii) the prolonged existence of long chain oligogalacturonides (OGs). These oligosaccharides are able to elicit a general plant defense response, enabling the plant to further retard or curb the spread of infection. The main objective of this study was to investigate the regulatory aspects underlying PGIP expression in grapevine. Unlike most characterized PGIP encoding genes from other dicotyledonous plant species, no evidence to support the existence of a V. vinifera PGIP multigene family could be found from either genetic or biochemical analyses. Recently, a genomic DNA fragment from Vitis vinifera cv Pinotage was pathogen interactions with regards to the fundamental processes underlying defense gene regulation.
AFRIKAANSE OPSOMMING: Die ooglopende voordele wat, vanuit 'n landboukundige én ekonomiese oogpunt, uit siekte- en stresbestande plante spruit, het gedurende die laaste dekade aanleiding gegee tot die ontwikkeling van plantpatogeen-interaksies as "n baie belangrike studieveld. Dit was dus ook te verwagte dat fundamentele navorsing in hierdie area nie net beperk gebly het tot modelorganismes soos Arabidopsis en tabak (ook natuurlik van landboukundige belang) nie, maar ook na meer tradisionele landbougewasse soos mielies, boontjies, sojaboontjies, appels, druiwe, ens. oorgevloei het. Verskeie siekteweerstands-verwante gene is byvoorbeeld al vanuit wingerd geïsoleer. Een só "n geen wat vir "n poligalakturonase-inhiberende proteïen (PGIP) kodeer, vorm deel van hierdie groep gene. Die funksie en regulering van PGIP's is baie goed bestudeer. Hierdie proteïene word normaalweg in die selwande van die meeste dikotiele plante aangetref. Leusienryke herhalings is algemeen in PGIP's en hierdie tipe van herhalings is kenmerkend van proteïene betrokke by proteïen-proteïen-interaksies. Verder word pgip-gene gewoonlik in klein multigeenfamilies aangetref, waar in die meeste gevalle die uitdrukking weefselspesifiek en die regulering spesifiek ten opsigte van die ontwikkelingsfase is. Verskeie faktore kan tot die induksie van pgip-gene lei, soos onder andere patogeen-infeksie, elisitoor-, salisiensuur-, jasmoonsuur- en kouebehandeling, asook verwonding. Differensiële regulering word in baie gevalle tussen lede van dieselfde multigeenfamilie aangetref. Hierdie differensiële regulering kan selfs bemiddel word deur onafhanklike reguleringsweë in reaksie op dieselfde induksiestimulus. PGIP's is in staat om te reageer met poligalakturonases (PGs), wat selwande afbreek en wat gedurende die infeksieproses deur swamme of fungi afgeskei word. Die effek van hierdie interaksie is tweeledig: (i) Die fisiese interaksie tussen PGIP en PG moduleer die aktiwiteit van die PG deur die ensiemaksie te inhibeer, en (ii) PGinhibisie lei tot die verhoogde stabiliteit van langketting-oligogalakturonades, molekules wat daartoe in staat is om die weerstandsrespons van plante te ontlok. Die inhibisie van die patogeen-PG's, tesame met die geïnduseerde weerstandrespons, stel die plant dan in staat om verdere infeksie te vertraag of te verhoed. Die doel van hierdie studie was om die onderliggende aspekte van PGIPregulering in wingerd te bestudeer. In teenstelling met die meeste plantspesies waar pgip-gene in klein multigeenfamilies aangetref word, is daar nie 'n pgip-multigeenfamilie in wingerd nie. Veelvuldige kopieë van In enkele pgip-geen word egter in die wingerdgenoom aangetref. Daar is onlangs in ons laboratorium In genoom-DNAfragment vanaf Vitis vinifera cv Pinotage geïsoleer wat die oopleesraam en 5'-stroomopsekwense van In PGIP-enkoderende geen (Vvpgip1) bevat. In hierdie studie is die uitdrukkingspatroon van Vvpgip1 ten opsigte van weefselspesifisiteit, korrelontwikkelingsfase, asook die effek van verskeie omgewings en patogeenverwante stres-stimuli ontleed. Die regulatoriese meganismes van Vvpgip1 bevat spesifieke in planta-ontwikkelingsfaseseine wat verder deur spesifieke faktore, insluitende omgewings- en patogeenstres, gereguleer word. In lyn hiermee is mRNS-transkripte van Vvpgip1 tot wortel- en korrelweefsels beperk, terwyl die mRNS-vlakke ook tussen verskillende korrelontwikkelingsfases wissel. Kumulatiewe uitdrukking kon waargeneem word in veráison-korrels in reaksie op verwonding en osmotiese stres. Die weefselspesifieke uitdrukkingspatroon tipies van wingerd-PGIP is in blare opgehef in reaksie op Botrytis cinerea-infeksie, verwonding, osmotiese stres, ouksien (indoolasynsuur) en salisiensuur. PGIP-uitdrukking word ook onderdruk deur In staurosporien-sensitiewe proteïenkinase, wat In goeie aanduiding is van die betrokkenheid van proteïenfosforilasie in die seintransduksiekaskade wat tot PGIPuitdrukking aanleiding gee. Die geïnduseerde PGIP-uitdrukkingsprofiel in wingerdblare kan ook nageboots word in tabak wat met die Vvpgip1-geen en -promotor getransformeer is. PG-inhibisie-eksperimente met membraan-geassosieerde proteïenekstrakte van geïnduseerde wingerdblare het ook dieselfde profiel getoon as dié van PGIP wat deur die Vvpgip1-geen geënkodeer is. Die uitdrukkingsprofiel van PGIP in die transgeniese tabakplante het ook bewys dat die promotor van die Vvpgip1-geen vir die geïnduseerde PGIP-uitdrukkingsprofiel in wingerdblare verantwoordelik is. In silica-analise van die promotorarea dui op die teenwoordigheid van verskeie cis-werkende elemente. Die kern promotor en transkripsie-aanvangsgedeelte is gevolglik eksperimenteel bepaal. Verder het uitdrukkingseksperimente met promotorfragmente verskeie dele van die promotor geïdentifiseer wat by stimulis-geassosieerde uitdrukking betrokke is. Posisioneel is hierdie fragmente in goeie konteks met die voorspelde cis-werkende elemente en kan dus die basis vorm vir verdere studies oor Vvpgip-regulering. Met hierdie studie word die eerste data verskaf waar die regulering van PGIP deur omgewingsverwante faktore verbind kan word met onwikkelingspesifieke toestande in die plant. Verder verskaf die resultate verdere bewyse vir die rol van PGIP in plant-patogeen-interaksies en lewer spesifieke bydraes tot die onderliggende prosesse wat by die regulering van siekteweerstandverwante gene betrokke is.

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5

Becker, John van Wyk. "Plant defence genes expressed in tobacco and yeast". Thesis, Stellenbosch : University of Stellenbosch, 2002. http://hdl.handle.net/10019/2924.

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6

Sassi, Giovanna. "Relative quantification of host gene expression and protein accumulation upon turnip mosaic potyvirus infection in tobacco". Thesis, McGill University, 2004. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=81433.

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Abstract (sommario):

Turnip mosaic virus (TuMV) infects a variety of crops, worldwide, including the economically relevant Brassicacea family. It was previously demonstrated that TuMV infection in tobacco protoplasts leads to an overall decrease of host protein. However, it remains unclear whether this phenomenon is due to the repression of plant gene transcription during the infection period or due to viral inhibition of host translation. In this study, quantification of various transcripts and protein products from infected tobacco was performed via real-time RT-PCR and ELISA. In comparison to the gamma-tubulin endogenous control, gene expression for the tobacco H3, HSP70 and granule-bound starch synthase was affected by TuMV infection with time.
Tobacco protein accumulation in whole leaf tissues was also significantly affected by increase of virus particles.

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7

Ozumit, Alen. "Interaction between turnip mosaic potyvirus (TuMV) cylindrical inclusion protein and Arabidopsis thaliana histone H3 protein". Thesis, McGill University, 2003. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=79060.

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Turnip mosaic potyvirus (TuMV) is a single-stranded RNA plant virus. One of its proteins, the cylindrical inclusion (CI) protein, was hypothesized to interfere with host transcription via interaction with histone H3 protein. Interaction between CI and histone H3 was previously observed in Dr. Fortin's laboratory. Based on previous studies that demonstrated the importance of the H3 tail domain in gene regulation and chromosome arrangement, it was hypothesized that CI would interact with the tail rather than the globular domain. The objective of this project was to identify which histone H3 domains CI protein interacts with. The full-length, globular, and tail domains of histone H3 DNA were expressed in E. coli and purified. Based on in vitro interaction experiments, the CI protein was observed to interact with the globular domain of histone H3.

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8

Zhan, Ye. "Molecular analysis of turnip crinkle virus coat protein mutations". Link to electronic thesis, 2002. http://www.wpi.edu/Pubs/ETD/Available/etd-0430102-142639.

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9

Phelan, Thomas Joseph. "GENETIC AND MOLECULAR ANALYSIS OF PLANT NUCLEAR MATRIX PROTEINS". NCSU, 2001. http://www.lib.ncsu.edu/theses/available/etd-20011104-233111.

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PHELAN, THOMAS JOSEPH, Genetic and Molecular Analysis of Plant Nuclear Matrix Proteins. (Under the direction of Steven L. Spiker.)The eukaryotic nucleus is composed of DNA, RNA and protein, encapsulated by a nuclear envelope. DNA is compacted up to ten thousand times in order to be packaged into the nucleus. The nucleus must maintain order in the presence of a very high density and variety of protein and RNA. The nuclear matrix is a proteinaceous network thought to provide structure and organization to the nucleus. We believe that relatively stable interactions of nuclear molecules with the nuclear matrix are key to organization of the nucleus. Numerous "Matrix Attachment Region" DNA elements (MARs), have been isolated from plants, animals, and fungi. Evidence suggests that these MARs attach to the nuclear matrix, delimiting loops of chromosomal DNA. In studies of transgenic plants and animals, MARs have been shown to give important advantages to organisms transformed with genes flanked by these elements. Unlike most DNA elements, no specific sequence elements have been identified in MAR DNAs. Partly due to the insolubility of the matrix, and to the heterogeneity of MAR DNA, very few of the protein components of the nuclear matrix have been identified. This work presents analysis the proteins of the plant nuclear matrix. We have characterized a set of related proteins from the model plant Arabidopsis that associate with MAR DNA in vitro. These proteins appear to be similar to the NOP56/NOP58 family of proteins previously identified in several eukaryotic organisms. The NOP56/NOP58 proteins are thought to be involved in modifications of ribosomal RNA. Binding studies presented in this work suggest that these plant proteins may participate in RNA/DNA/protein complexes in the nucleus.

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Tan, Lor-Wai. "Biochemical aspects of self-incompatibility in Petunia hybrida". Title page, Contents and Summary only, 1988. http://web4.library.adelaide.edu.au/theses/09A/09at161.pdf.

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Libri sul tema "Plant proteins Genetic aspects"

1

Bharló, Gillian Ní. Cloning and characterisation of an Auxin-binding protein cDNA from apple. Dublin: University College Dublin, 1997.

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2

International Symposium on Genetic Aspects of Plant Mineral Nutrition (4th 1991 Canberra, A.C.T.). Genetic aspects of plant mineral nutrition. Dordrecht: Kluwer Academic Publishers, 1993.

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3

El Bassam, N., M. Dambroth e B. C. Loughman, a cura di. Genetic Aspects of Plant Mineral Nutrition. Dordrecht: Springer Netherlands, 1990. http://dx.doi.org/10.1007/978-94-009-2053-8.

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Gabelman, W. H., e B. C. Loughman, a cura di. Genetic Aspects of Plant Mineral Nutrition. Dordrecht: Springer Netherlands, 1987. http://dx.doi.org/10.1007/978-94-009-3581-5.

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Randall, P. J., E. Delhaize, R. A. Richards e R. Munns, a cura di. Genetic Aspects of Plant Mineral Nutrition. Dordrecht: Springer Netherlands, 1993. http://dx.doi.org/10.1007/978-94-011-1650-3.

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6

International, Symposium on Genetic Aspects of Plant Mineral Nutrition (2nd 1985 Madison Wis ). Genetic aspects of plant mineral nutrition: Proceedings of the Second International Symposium on Genetic Aspects of Plant Mineral Nutrition. Dordrecht: M. Nijhoff, 1987.

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John, King. The genetic basis of plant physiological processes. New York: Oxford University Press, 1991.

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Todorov, I. N. Mechanisms of cell stability: Subcellular and molecular aspects. New York: Nova Science Publishers, 1994.

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9

Guinivan, Phyllis. Selected abstracts on oncogene protein products. Bethesda, MD: U.S. Dept. of Health and Human Services, Public Health Service, National Institutes of Health, International Cancer Research Data Bank, National Cancer Institute, 1987.

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10

Gustavsson, Hans-Olof. Studies on the expression of the seed storage proteins napin and cruciferin from Brassica napus. Uppsala, Sweden: Swedish University of Agricultural Sciences, Uppsala Genetic Center, Dept. of Cell Research, 1994.

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Capitoli di libri sul tema "Plant proteins Genetic aspects"

1

Flowers, T. J., e D. Dalmond. "Protein synthesis in halophytes: The influence of potassium, sodium and magnesium in vitro". In Genetic Aspects of Plant Mineral Nutrition, 195–203. Dordrecht: Springer Netherlands, 1993. http://dx.doi.org/10.1007/978-94-011-1650-3_25.

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2

Memon, Abdul Razaque, e Anthony D. M. Glass. "Genotypic differences in subcellular compartmentation of K+: Implications for protein synthesis, growth and yield". In Genetic Aspects of Plant Mineral Nutrition, 323–29. Dordrecht: Springer Netherlands, 1987. http://dx.doi.org/10.1007/978-94-009-3581-5_30.

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3

Wang, Melan, e Katalin A. Hudak. "Applications of Plant Antiviral Proteins". In Genetic Engineering, 143–61. Boston, MA: Springer US, 2003. http://dx.doi.org/10.1007/978-1-4615-0073-5_7.

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4

Robinson, C. "Targeting of proteins to chloroplasts and mitochondria". In Plant Genetic Engineering, 179–98. Dordrecht: Springer Netherlands, 1991. http://dx.doi.org/10.1007/978-94-009-0403-3_6.

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5

Robinson, C. "Targeting of proteins to chloroplasts and mitochondria". In Plant Genetic Engineering, 179–98. Dordrecht: Springer Netherlands, 1991. http://dx.doi.org/10.1007/978-94-010-9646-1_6.

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6

Payne, Peter I. "Endosperm Proteins". In A Genetic Approach to Plant Biochemistry, 207–31. Vienna: Springer Vienna, 1986. http://dx.doi.org/10.1007/978-3-7091-6989-6_7.

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7

Koornneef, Maarten. "Genetic Aspects of Abscisic Acid". In A Genetic Approach to Plant Biochemistry, 35–54. Vienna: Springer Vienna, 1986. http://dx.doi.org/10.1007/978-3-7091-6989-6_2.

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8

Hill, J., H. C. Becker e P. M. A. Tigerstedt. "Genetic resources, genetic diversity and ecogeographic breeding". In Quantitative and Ecological Aspects of Plant Breeding, 235–67. Dordrecht: Springer Netherlands, 1998. http://dx.doi.org/10.1007/978-94-011-5830-5_9.

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9

Bos, Izak, e Peter Caligari. "Population genetic aspects of cross-fertilization". In Selection Methods in Plant Breeding, 5–25. Dordrecht: Springer Netherlands, 1995. http://dx.doi.org/10.1007/978-94-015-8432-6_2.

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10

Randhawa, G., J. Lyon, N. Harris, H. V. Davies e G. C. Machray. "Manipulation of Potato Tuber Protein Quality through Genetic Engineering". In Plant Proteins from European Crops, 70–74. Berlin, Heidelberg: Springer Berlin Heidelberg, 1998. http://dx.doi.org/10.1007/978-3-662-03720-1_12.

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Atti di convegni sul tema "Plant proteins Genetic aspects"

1

Kholodnyak, Oleksandr, e Svitlana Pavlova. "THE CONSERVATION AND MANAGEMENT OF PLANT GENETIC RESOURCES". In THEORETICAL AND PRACTICAL ASPECTS OF MODERN SCIENTIFIC RESEARCH. European Scientific Platform, 2021. http://dx.doi.org/10.36074/logos-30.04.2021.v1.38.

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2

"Biochemical, molecular and genetic aspects of fruit ripening in green-fruited and red-fruited tomato species". In Plant Genetics, Genomics, Bioinformatics, and Biotechnology. Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, 2019. http://dx.doi.org/10.18699/plantgen2019-179.

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3

Puzansky, R. K., e M. F. Shishova. "Metabolomic and molecular genetic aspects of trophic adaptation of mutants Chlamydomonas reinhardtii". In IX Congress of society physiologists of plants of Russia "Plant physiology is the basis for creating plants of the future". Kazan University Press, 2019. http://dx.doi.org/10.26907/978-5-00130-204-9-2019-366.

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4

Tyapkina, D. Yu, E. Z. Kochieva e M. A. Slugin. "Biochemical and molecular genetic aspects of the metabolism of L-ascorbic acid invarieties and wild species of tomato (Solanum sect. Lycopersicon)". In IX Congress of society physiologists of plants of Russia "Plant physiology is the basis for creating plants of the future". Kazan University Press, 2019. http://dx.doi.org/10.26907/978-5-00130-204-9-2019-445.

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5

Tsyganov, V. E. "Symbiotic nodule development". In 2nd International Scientific Conference "Plants and Microbes: the Future of Biotechnology". PLAMIC2020 Organizing committee, 2020. http://dx.doi.org/10.28983/plamic2020.257.

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Abstract (sommario):

The interaction of legumes with rhizobia leads to formation of the symbiotic nodules on their roots, which are specialized plant organs for nitrogen fixation. Considerable progress has been made in deciphering the molecular-genetic and cellular mechanisms of symbiotic nodule development in recent years. However, some aspects of nodule development clearly merit much more attention.

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Rapporti di organizzazioni sul tema "Plant proteins Genetic aspects"

1

Siedow, J. (Molecular studies of functional aspects of plant mitochondrial proteins): (Annual performance report for 1988--1989). Office of Scientific and Technical Information (OSTI), gennaio 1989. http://dx.doi.org/10.2172/5963065.

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