Letteratura scientifica selezionata sul tema "Phomopsis"
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Articoli di riviste sul tema "Phomopsis"
Croker, KP, e MA Johns. "Reproductive performance of ewes fed sweet narrow-leafed lupin seed infected with Phomopsis leptostromiformis". Australian Journal of Experimental Agriculture 25, n. 3 (1985): 536. http://dx.doi.org/10.1071/ea9850536.
Testo completoKsiążkiewicz, M., K. Wójcik, W. Irzykowski, W. Bielski, S. Rychel, J. Kaczmarek, P. Plewiński, E. Rudy e M. Jędryczka. "Validation of Diaporthe toxica resistance markers in European Lupinus angustifolius germplasm and identification of novel resistance donors for marker-assisted selection". Journal of Applied Genetics 61, n. 1 (22 ottobre 2019): 1–12. http://dx.doi.org/10.1007/s13353-019-00521-y.
Testo completoWood, PM, DS Petterson, GR Hancock e GA Brown. "Distribution of seed infected with Phomopsis leptostromiformis and of phomopsin A within a lupin crop". Australian Journal of Experimental Agriculture 27, n. 1 (1987): 77. http://dx.doi.org/10.1071/ea9870077.
Testo completoTuset, J. J., e M. A. T. Portilla. "Taxonomic status of Fusicoccum amygdali and Phomopsis amygdalina". Canadian Journal of Botany 67, n. 5 (1 maggio 1989): 1275–80. http://dx.doi.org/10.1139/b89-168.
Testo completoPetterson, DS, JE Peterson, LW Smith, PM Wood e CCJ Culvenor. "Bioassay of the contamination of lupin seed by the mycotoxin phomopsin". Australian Journal of Experimental Agriculture 25, n. 2 (1985): 434. http://dx.doi.org/10.1071/ea9850434.
Testo completoVukojević, J., M. Mihaljčević e D. Franić-Mihajlović. "VARIABILITY OF Phomopsis POPULATIONS IN SUNFLOWER (Helianthus annuus L.) / VARIABILIDAD DE LAS POBLACIONES DE Phomopsis EN EL GIRASOL (Helianthus annuus L.) / VARIABILITÉ DES POPULATIONS DE Phomopsis CHEZ LE TOURNESOL (Helianthus annuus L.)". HELIA 24, n. 34 (luglio 2001): 69–76. http://dx.doi.org/10.1515/helia.2001.24.34.69.
Testo completoRadiastuti, Nani, Firdaus Ramadhan e Yusraini Dian Inayati Siregar3. "Antioksidan Ekstrak Kapang Endofit Phomopsis spp. dari Tanaman Kina (Cinchona calisaya)". Jurnal Biotek Medisiana Indonesia 10, n. 2 (18 gennaio 2022): 109–15. http://dx.doi.org/10.22435/jbmi.v10i2.5822.
Testo completoMurali, T. S., T. S. Suryanarayanan e R. Geeta. "Endophytic Phomopsis species: host range and implications for diversity estimates". Canadian Journal of Microbiology 52, n. 7 (1 luglio 2006): 673–80. http://dx.doi.org/10.1139/w06-020.
Testo completoLi, Shuxian, Glen L. Hartman e Deborah L. Boykin. "Aggressiveness of Phomopsis longicolla and Other Phomopsis spp. on Soybean". Plant Disease 94, n. 8 (agosto 2010): 1035–40. http://dx.doi.org/10.1094/pdis-94-8-1035.
Testo completoWrather, J. A., D. A. Sleper, W. E. Stevens, J. G. Shannon e R. F. Wilson. "Planting Date and Cultivar Effects on Soybean Yield, Seed Quality, and Phomopsis sp. Seed Infection". Plant Disease 87, n. 5 (maggio 2003): 529–32. http://dx.doi.org/10.1094/pdis.2003.87.5.529.
Testo completoTesi sul tema "Phomopsis"
Jellal, Abdelilah. "Production, isolement et purification de deux chaetoglobosines produites par le champignon Phomopsis leptostromiformis : études structurale et toxicologique". Paris 7, 1993. http://www.theses.fr/1993PA077331.
Testo completoScheper, Reiny W. A. "Studies on the biology and genetic variation of phomopsis on grapevine /". Title page, contents and abstract only, 2001. http://web4.library.adelaide.edu.au/theses/09PH/09phs325.pdf.
Testo completoMiric, Elizabeth. "Pathological, morphological and molecular studies of a worldwide collection of the sunflower pathogens phomopsis helianthi and phoma macdonaldii /". St. Lucia, Qld, 2002. http://www.library.uq.edu.au/pdfserve.php?image=thesisabs/absthe16548.pdf.
Testo completoNita, Mizuho. "Epidemiology and management of Phomopsis cane and leaf spot of grape". Connect to resource, 2005. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=osu1125542230.
Testo completoTitle from first page of PDF file. Document formatted into pages; contains xviii, 163 p.; also includes graphics (some col.). Includes bibliographical references (p. 156-163). Available online via OhioLINK's ETD Center
Mostert, Lizel. "The characterization and control of Phomopsis cane and leaf spot on vine". Thesis, Stellenbosch : Stellenbosch University, 2000. http://hdl.handle.net/10019.1/51945.
Testo completoENGLISH ABSTRACT: Phomopsis cane and leaf spot disease of grapevine is an economically important disease in many of the vine-growing areas of the world. Four different Phomopsis spp. have previously been associated with this disease. The present study investigates the taxonomic significance of the different taxa found on grapevines in South Africa, as well as the endophytic growth and fungicide sensitivity of Phomopsis viticola isolates. The thesis is compiled of several different parts, which deal with specific, but related topics, and hence some duplication has been unavoidable. Understanding the epidemiology of a disease is important for the correct timing of disease control. To investigate the endophytic growth of P. viticola, asymptomatic shoots were collected at eight different growth stages. Nodes, internodes, leaf petioles, leaves, tendrils and bunch peduncles were investigated. Two Phomopsis spp., taxon 1 and 2 were identified in this study. The Phomopsis viticola-complex had a relative importance of 9% and accounted for 3% of the isolations. P. viticola (taxon 2) is mainly isolated from the nodes and internodes. Inoculations of healthy, young vine tissue confirmed taxon 2 to be a virulent pathogen, suggesting that it is a latent pathogen rather than an endophyte. In contrast, taxon 1 appeared to be a true endophyte, and did not seem to be an important pathogen on vines. The true identity of the causal organism of Phomopsis cane and leaf spot disease was investigated by collecting samples from 58 different vineyards in the grapevine growing areas of the Western Cape. P. viiicola occurred in grapevine material collected from Lutzville to Swellendam, but was not found in the Oudtshoorn and Orange River grapevine areas. Diaporthe perjuncta (taxon 1), P. vutcola (taxon 2), taxon 3 and a Phomopsis species commonly associated with shoot blight of peaches in the U.S.A., P. amygdali, were identified among the South African grapevine isolates. Examination of the Australian culture designated as taxon 4 found it to be a species of Libertella, thus excluding it from the P. viticola-complex. An Italian isolate was found to represent a species of Phomopsis not previously known from grapevines, and this was subsequently described as taxon 5. Species delimitation was based on morphological and cultural characteristics, stem inoculations and the formation of the teleomorph in vitro. The identity of each morphological taxon was confirmed by means of phylogenetic analyses of the nuclear ribosomal DNA internal transcribed spacers (ITS 1 and ITS2) and the 5' end partial sequence of the mitochondrial small subunit (mtSSU). P. amygdali, associated with peach shoot blight in the U.S.A., was isolated once only and appeared to be of lesser importance in this disease complex. Furthermore, taxa 1 (Diaporthe perjuncta) and 3 were also rarely encountered and proved to be non-pathogenic, indicating their non-functional role in Phomopsis cane and leaf spot disease. Taxon 2 (Phomopsis viticolas was common and widely distributed in diseased vineyards. This taxon was associated with the typical disease symptoms and proved to be pathogenic. Morphologically taxon 2 corresponded best with P. viticola, which was also neotypified in this study. Taxon 2 was mostly isolated from buds and nodes, indicating that these are important sites in which the fungus survives during winter. Molecular data indicated that taxon 3 and P. amygdali were not host specific to grapevine. The currently used foliar fungicides were compared to the new strobilurin fungicides. The effects of nine fungicides (azoxystrobin, flusilazole, folpet, fosetyl- Al+mancozeb, kresoxim-methyl, mancozeb, penconazole, spiroxamine and trifloxystrobin) were tested in vitro on inhibition of mycelial growth. The following EC50 (ug/ml) values were obtained: azoxystrobin (0.350), flusilazole (0.007), folpet (4.489), fosetyl-Al+mancozeb (3.925), kresoxim-methyl (1.665), mancozeb (2.891), penconazole (0.023), spiroxamine (0.321) and trifloxystrobin (0.051). Additionally, azoxystrobin, folpet, kresoxim-methyl, mancozeb, propineb and trifloxystrobin were tested for their ability to inhibit spore germination in vitro. The subsequent EC50 (ug/ml) values were obtained: azoxystrobin 0.123), folpet (0.510), kresoxim-methyl (0.0037), mancozeb (0.250), propineb (0.156) and trifloxystrobin (0.003). The results reported in part 4 showed that the strobilurin fungicides inhibited the mycelial growth and spore germination of P. viticola. However, further trials need to be conducted to verify these findings under field conditions. In the present study taxa 1, 3 and P. amygdali were infrequently isolated, suggesting that they played a less prominent role in the P. viticolacomplex.
AFRIKAANSE OPSOMMING: Streepvleksiekte van wingerd is 'n ekonomies belangrike siekte wat in die meeste wingerdproduserende gebiede van die wêreld voorkom. Vier Phomopsis spesies is in die verlede met dié siekte geassosieer. Hierdie studie ondersoek die taksonomiese belangrikheid van die verskillende taksa wat op wingerd in Suid Afrika gevind word, asook die endofietiese groei en fungisiedsensitiwiteit van die Phomopsis vitico/a isolate. Hierdie tesis bestaan uit verskeie dele met spesifieke, maar verwante onderwerpe wat tot onafwendbare duplisering lei. Dit is belangrik om die epidemiologie van 'n siekte te verstaan sodat korrekte en tydsberekende siektebeheer toegepas kan word. Die endofietiese groei van P. vitico/a is ondersoek deur simptoomlose lote by agt verskillende groei stadiums te versamel. Nodusse, internodusse, blaarstele, blare, rankies en trosstele is ondersoek. Twee Phomopsis spp., takson 1 en 2 is geïdentifiseer. Die Phomopsis vitico/a-kompleks het 3% van die isolasies uitgemaak en 'n relatiewe belangrikheid van 9% getoon. P. vitico/a (takson 2) is meestal uit die nodus en internodus geïsoleer. lnokulasies van gesonde, jong wingerdweefsel het bevestig dat takson 2 'n virulente patogeen is en dat die takson eerder 'n latente patogeen as 'n endofiet is. In teenstelling hiermee is takson 1 'n ware endofiet en 'n onbelangrike patogeen op wingerd. Die ware identiteit van die veroorsakende organisme van streepvlek is ondersoek deur plantmateriaal vanaf 58 verskillende wingerde in die wingerproduserende gebiede van die Wes-Kaap te versamel. P. vitico/a is in wingerdmateriaal vanaf Lutzville tot Swellendam aangetref, maar nie in die Oudtshoorn en Oranjerivier wingerd produserende gebiede nie. Diaporthe perjuncta (takson 1), P. vitico/a (takson 2), takson 3 en P. amygdali is in die Suid Afrikaanse wingerdisolate geïdentifiseer. P. amygdali word met lootverskroeiing van perske bome in die V.S.A. geassosieer. Die Australiese isolaat wat benoem is as takson 4, is met die huidige ondersoek gevind om 'n spesie van Libertella te wees. Takson 4 is daarvolgens uit die P. vitico/a-kompleks gelaat. 'n Italiaanse isolaat het 'n nuwe spesie van Phomopsis op wingerd verteenwoordig en is vervolgens as takson 5 beskryf. Spesie-onderskeiding is op morfologiese en kulturele eienskappe, staminokulasies en die vorming van die teleomorf in vitro gebaseer. Die identiteit vanelke morfologiese takson is met behulp van filogenetiese analises van die nukleêre ribosomale DNS intern transkriberende spasieerders (ITS 1 en ITS2) en die 5' punt gedeeltelike nukleotied volgorde van die mitochondriale klein subeenheid (mtSSU) bevestig. P. amygdali is slegs een keer geïsoleer en blyk van minder belang in die siektekompleks te wees. Takson 1 (Diaporthe perjuneta) en takson 3 het ook min voorgekom en is nie-patogenies, wat hul nie-funksionele rol in streepvleksiekte aandui. Takson 2 (P. viticola) is algemeen geïsoleer en kom wyd verspreid voor. Hierdie takson is geassosieer met die tipiese siektesimptome en is ook patogenies. Morfologies stem takson 2 met P. viiicola ooreen en is ook geneotipifiseer in hierdie studie. Takson 2 is meestal vanaf die ogies en nodusse geïsoleer, wat daarop dui dat hierdie belangrike setels is waar die swam tydens die winter oorleef. Die molekulêre data toon aan dat takson 3 en P. amygdali nie gasheerspesifiek tot wingerd is nie. Die swamdoders wat tans teen streepvlek gebruik word, is met die nuwe strobilurin swamdoders vergelyk. Die effek van nege swamdoders (azoksistrobin, flusilasool, folpet, fosetyl-Al + mancozeb, kresoxirn-metiel, mankozeb, penconasool, spiroksamien en trifloksistrobin) is in vitro op die inhibisie van miseliumgroei getoets. Die volgende EKso-waardes (g/ml) is verkry: azoxystrobin (0.350), flusilasool (0.007), folpet (4.489), fosetiel-Al + mankozeb (3.925), kresoxirn-metiel (l.665), mankozeb (2.891), penkonasool (0.023), spiroksamien (0.321) en trifloxystrobin (0.051). Azoxystrobin, folpet, kresoxim-rnetiel, mankozeb, propineb en trifloksistrobin is ook in vitro getoets vir hul inhibisie op spoorontkieming. Die volgende EKso-waardes is verkry: azoxystrobin (0.123), folpet (0.510), kresoxim-metiel (0.0037), mankozeb (0.250), propineb (0.156) en trifloxystrobin (0.003). Die resultate vervat in deel 4 toon dat die strobilurin swamdoders die miseliumgroei en spoorontkieming van P. viticola inhibeer. Toetsing in die veld word egter benodig om die effektiwiteit van die middels te bevestig. In hierdie studie is taksa I, 3 en P. amygdali selde geïsoleer, wat aangedui het dat hierdie taksa 'n minder belangrike rol in die P. viticola-kompleks speel.
Garcia, Armando da Costa, e 92-99157-4536. "Avaliação química e biológica do extrato bruto do fungo endofítico Phomopsis sp". Universidade Federal do Amazonas, 2017. http://tede.ufam.edu.br/handle/tede/6219.
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FAPEAM - Fundação de Amparo à Pesquisa do Estado do Amazonas
Endophytic fungi are microorganisms that live in interne tissues of plants, without causing them immediate damage. They are considered an important source to the production of secondary metabolites with antimicrobial, antitumor activities, among others. The present work describes the chemical and biological study of the secondary metabolites produced by the endophytic fungus Phomopsis sp. isolated from the plant Passovia stelis. For that, the fungus at hand was cultivated in large scale using the liquid medium of dextrose potato. The ethylacetat crude extract was obtained through extraction from the broth and then submitted to larvicide assay against larvae of the mosquito Aedes aegypti and antimicrobial against the pathogenic bacteria Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli and Pseudomonas aeruginosa. It was also performed assays against the enzyme anticholinesterase, antioxidants and antifungal against Cladosporium cladosporioides e Cladosporium sphaerospermum. In the selective larvicide, 3 fractions showed bioactivity (100 ppm) in 24h. In the dosing assay larvicide, the extract of the fungus Phomopsis sp. showed bioactivity with LD50 of 84.7, 81.6 and 79.5 ppm and LD90 de 147.8, 140.2 and 137.7 ppm after 24, 48 e 72 h, respectively. The crude extract was analyzed using TLC, HPLC and NMR and then purified through column chromatography and HLPC-DAD. From F4, the 3-nitropropionic acid was isolated, which is used in neurological research. Succinic acid, which is considered one of the construction blocks in chemical synthesis industry, was isolated from the fraction F7. Other 3 esterified substances, derivatives of tyrosol, were isolated of the fraction F1, and one of them unpublished. In the antioxidant assay, two fractions (F5, F6) showed positive results and in the assay against the enzyme anticholinesterase four fraction (F2, F5, F6, F7) presented bioactivity. These results demonstrate the expressive biopotency of the fungus Phomopsis sp. and open a new perspective for further studies.
Fungos endofíticos são fungos que vivem no tecido interno das plantas sem que lhes causem prejuízo imediato. Esses microrganismos são considerados uma fonte importante para a produção de metabólitos secundários com ação antimicrobiana, antitumoral, entre outras. Este trabalho descreve o estudo químico e biológico dos metabólitos secundários produzidos pelo fungo endofítico Phomopsis sp. isolado da planta Passovia stelis. Para tanto, o fungo em questão foi cultivado em escala ampliada, utilizando meio líquido de batata dextrose. O caldo obtido foi extraído com acetato de etila fornecendo o extrato bruto. Este foi submetido à ensaio larvicida, frente as larvas do mosquito Aedes aegypti, antimicrobiano frente às bactérias patogênicas Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli e Pseudomonas aeruginosa. Também foram realizados ensaios anticolinesterase, antioxidante e antifúngico frente aos fungos Cladosporium cladosporioides e Cladosporium sphaerospermum. No ensaio larvicida de dose, o extrato do fungo Phomopsis sp. exibiu bioatividade com valores de DL50 de 84,7; 81,6 e 79,5 ppm e DL90 de 147,8; 140,2 e 137,7 ppm respectivamente, nas leituras realizadas após 24, 48 e 72 h. No ensaio larvicida seletivo, três frações obtiveram bioatividade em 100 ppm nas primeiras 24 horas. Após análise por CCD, CLAE e RMN, o extrato bruto foi fracionado por cromatografia em coluna e por CLAE-DAD. Da fração F4 foi isolado o ácido 3-nitropropiônico que é muito utilizado em pesquisas neurológicas. Da fração F7 foi isolado o ácido succínico tido como um dos principais blocos de construção na indústria da síntese química. Outras três substâncias da classe dos ésteres, derivados do tirosol foram isolados da fração F1 sendo uma delas inédita. No ensaio antioxidante duas frações (F5, F6) deram resultado positivo e no ensaio anticolinesterásico quatro frações (F2, F5, F6, F7) mostraram atividade. Estes resultados mostraram a expressiva biopotência do fungo Phomopsis sp, abrindo novas perspectivas atrativas para estudos futuros.
Yu, Haiqian [Verfasser]. "Secondary Metabolites from Fungi Coniella fragariae and Phomopsis sp. D15a2a / Haiqian Yu". Düsseldorf : Universitäts- und Landesbibliothek der Heinrich-Heine-Universität Düsseldorf, 2020. http://d-nb.info/120891507X/34.
Testo completoChapla, Vanessa Mara [UNESP]. "Estudo químico e biológico do fungo endofítico Phomopsis sp. isolado da Senna spectabilis". Universidade Estadual Paulista (UNESP), 2010. http://hdl.handle.net/11449/97895.
Testo completoCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Micro-organismos endofíticos habitam os espaços intra e intercelular de um hospedeiro, durante todo ou parte de seu ciclo de vida, sem causar danos aparentes. Esses micro- organismos são considerados uma fonte importante para a produção de metabólitos secundários com ação antimicrobiana, antitumoral, entre outras. Este trabalho descreve o estudo químico e biológico do fungo endofítico Phomopsis sp. associado à espécie vegetal do cerrado Senna spectabilis. Phomopsis sp. foi cultivado em diferentes meios de cultura para avaliar a variação metabólica em relação a composição dos nutrientes. Foram usados 5 meios líquidos comerciais (MBD, YM, Extrato de Malte, Nutrient e Czapek) e 2 meios sólidos (Milho e Arroz). Os extratos brutos Acetato e Acetonitrila obtidos foram avaliados por Cromatografia Líquida de Alta Eficiência com detector de arranjo de diodos e por Ressonância Magnética Nuclear de 1 H, e submetidos aos ensaios para a avaliação da potencialidade antifúngica, antioxidante e anticolinesterásica. Os resultados evidenciaram extratos com uma variação metabólica intensa e potencialmente bioativos. Destes, os meios MDB e Milho foram selecionados para obtenção dos extratos brutos em escala ampliada. O extrato bruto acetato obtido em MDB foi submetido a fracionamento cromatográfico e levou à identificação da uracila (1), ácido nitropropanóico (2), citocalasina H (3), tirosol (4), ciclo(L- Pro-L-Leu), (5), ciclo(L-Pro-L-Ile) (6), ciclo(L-Pro-L-Val) (7), ciclo(L-Pro-L-Tyr) (8) e ciclo(L-Pro-L-Phe) (9). Este é o primeiro relato de dicetopiperazinas obtidos de fungos do gênero Phomopsis. Esta classe de substâncias apresenta inúmeras bioatividades, principalmente antimicrobiana. O extrato bruto acetonitrila obtido de milho conduziu ao isolamento da citocalasina H (3), citocalasina J (10), alternariol monometil éter (11).... não descritos
Endophytic microorganisms inhabit the intra and intercellular spaces of a host, during all or part of their life cycle, without causing any apparent damage. Microorganisms are regarded as an important source for the production of secondary metabolites with antimicrobial, antitumoral actions, among others. This work describes the chemical and biological study of endophytic fungus Phomopsis sp. associated with plant species of Cerrado Senna spectabilis. Phomopsis sp. was cultived in different culture media to assess the metabolic variation in relation to nutrient composition. Were used 5 commercial liquid media (PDB, YM, Malt extract, nutrient and Czapek) and 2 solid mediums (Corn and Rice). The acetate and acetonitrile crude extracts were evaluated by High Performance Liquid Chromatography with Diode Array Detector and 1 H Nuclear Magnetic Resonance and subjected to assays for the evaluation of potential antifungal, antioxidant and anticholinesterase. The results showed extracts with an intense metabolic variation and potentially bioactive. From these, the mediums PDB and Corn were selected to obtain the crude extracts on a large scale. The crude acetate extract obtained in PDB was submitted to chromatographic separation and led to the identification of uracil (1), 3-nitropropionic acid (2), cytochalasin H (3), tyrosol (4), cyclo (L- Pro-L-Leu) (5), cyclo (L-Pro-L-Ile) (6), cyclo (L-Pro-L-Val) (7), cyclo (L-Pro-L-Tyr) (8) and cyclo (L-Pro-L-Phe) (9). This is the first report of diketopiperazines obtained from fungi of the Phomopsis genus. This class of substances shows many bioactivities, especially antimicrobial. The crude acetonitrile extract obtained from Corn led to the isolation of cytochalasin H (3), cytochalasin J (10), alternariol monomethyl ether (11), alternariol (12) and 2-hydroxy-alternariol (13), being the last one reported for the first time as a natural product and with NMR data not reported
Morin, Louise. "Development of the field bindweed bioherbicide, Phomopsis convolvulus : spore production and disease development". Thesis, McGill University, 1989. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=59614.
Testo completoIn controlled environment studies, a minimum of 18 hr of dew was required for severe disease development on inoculated plants. The addition of gelatin, Sorbo $ sp{ rm TM}$, or BOND$ sp{ rm TM}$ to the inoculum did not enhance the disease under various leaf wetness periods. A continuous dew period of 18 hr was superior to the cumulative effect of three interrupted 6 hr dew periods. Secondary inoculum was produced on diseased plants placed under moist conditions for 48 hr or more.
In greenhouse experiments, seedlings at the cotyledon and 3- to 5- leaf stage were severely diseased and killed when inoculated with 10$ sp9$ conidia/m$ sp2$. This inoculum density adversely affected the regenerative ability of 4 wk old seedlings and established plants, but few plants were killed. Inoculation of the healthy regrowth from plants previously inoculated with the fungus resulted in much less disease symptoms than expected.
Chapla, Vanessa Mara. "Estudo químico e biológico do fungo endofítico Phomopsis sp. isolado da Senna spectabilis /". Araraquara : [s.n.], 2010. http://hdl.handle.net/11449/97895.
Testo completoBanca: Dulce Helena Siqueira Silva
Banca: Jacqueline Aparecida Takahashi
Resumo: Micro-organismos endofíticos habitam os espaços intra e intercelular de um hospedeiro, durante todo ou parte de seu ciclo de vida, sem causar danos aparentes. Esses micro- organismos são considerados uma fonte importante para a produção de metabólitos secundários com ação antimicrobiana, antitumoral, entre outras. Este trabalho descreve o estudo químico e biológico do fungo endofítico Phomopsis sp. associado à espécie vegetal do cerrado Senna spectabilis. Phomopsis sp. foi cultivado em diferentes meios de cultura para avaliar a variação metabólica em relação a composição dos nutrientes. Foram usados 5 meios líquidos comerciais (MBD, YM, Extrato de Malte, Nutrient e Czapek) e 2 meios sólidos (Milho e Arroz). Os extratos brutos Acetato e Acetonitrila obtidos foram avaliados por Cromatografia Líquida de Alta Eficiência com detector de arranjo de diodos e por Ressonância Magnética Nuclear de 1 H, e submetidos aos ensaios para a avaliação da potencialidade antifúngica, antioxidante e anticolinesterásica. Os resultados evidenciaram extratos com uma variação metabólica intensa e potencialmente bioativos. Destes, os meios MDB e Milho foram selecionados para obtenção dos extratos brutos em escala ampliada. O extrato bruto acetato obtido em MDB foi submetido a fracionamento cromatográfico e levou à identificação da uracila (1), ácido nitropropanóico (2), citocalasina H (3), tirosol (4), ciclo(L- Pro-L-Leu), (5), ciclo(L-Pro-L-Ile) (6), ciclo(L-Pro-L-Val) (7), ciclo(L-Pro-L-Tyr) (8) e ciclo(L-Pro-L-Phe) (9). Este é o primeiro relato de dicetopiperazinas obtidos de fungos do gênero Phomopsis. Esta classe de substâncias apresenta inúmeras bioatividades, principalmente antimicrobiana. O extrato bruto acetonitrila obtido de milho conduziu ao isolamento da citocalasina H (3), citocalasina J (10), alternariol monometil éter (11).... não descritos
Abstract: Endophytic microorganisms inhabit the intra and intercellular spaces of a host, during all or part of their life cycle, without causing any apparent damage. Microorganisms are regarded as an important source for the production of secondary metabolites with antimicrobial, antitumoral actions, among others. This work describes the chemical and biological study of endophytic fungus Phomopsis sp. associated with plant species of Cerrado Senna spectabilis. Phomopsis sp. was cultived in different culture media to assess the metabolic variation in relation to nutrient composition. Were used 5 commercial liquid media (PDB, YM, Malt extract, nutrient and Czapek) and 2 solid mediums (Corn and Rice). The acetate and acetonitrile crude extracts were evaluated by High Performance Liquid Chromatography with Diode Array Detector and 1 H Nuclear Magnetic Resonance and subjected to assays for the evaluation of potential antifungal, antioxidant and anticholinesterase. The results showed extracts with an intense metabolic variation and potentially bioactive. From these, the mediums PDB and Corn were selected to obtain the crude extracts on a large scale. The crude acetate extract obtained in PDB was submitted to chromatographic separation and led to the identification of uracil (1), 3-nitropropionic acid (2), cytochalasin H (3), tyrosol (4), cyclo (L- Pro-L-Leu) (5), cyclo (L-Pro-L-Ile) (6), cyclo (L-Pro-L-Val) (7), cyclo (L-Pro-L-Tyr) (8) and cyclo (L-Pro-L-Phe) (9). This is the first report of diketopiperazines obtained from fungi of the Phomopsis genus. This class of substances shows many bioactivities, especially antimicrobial. The crude acetonitrile extract obtained from Corn led to the isolation of cytochalasin H (3), cytochalasin J (10), alternariol monomethyl ether (11), alternariol (12) and 2-hydroxy-alternariol (13), being the last one reported for the first time as a natural product and with NMR data not reported
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Libri sul tema "Phomopsis"
Harris, Jeri Lyn. Phomopsis blight at Bessey Nursery. Golden, CO: USDA Forest Service, Rocky Mountain Region, Renewable Resources, 1999.
Cerca il testo completoA world list of Phomopsis names with notes on nomenclature, morphology, and biology. Berlin: J. Cramer, 1988.
Cerca il testo completoFood, Ontario Ministry of Agriculture and. Phomopsis cane and leaf spot of grapes. S.l: s.n, 1990.
Cerca il testo completoFunk, A. Phomopsis (Diaporthe) canker of Douglas-fir in British Columbia. Victoria, B.C: Government of Canada, Canadian Forestry Service, Pacific Forestry Centre, 1986.
Cerca il testo completoFla.) Conference on the Diaporthe/Phomopsis Disease Complex of Soybean (1984 Fort Walton Beach. Proceedings of the Conference on the Diaporthe/Phomopsis Disease Complex of Soybean: March 26-27, 1984, Fort Walton Beach, Florida. Washington, D.C.?]: U.S. Dept. of Agriclture, Agricultural Research Service, 1985.
Cerca il testo completoCapitoli di libri sul tema "Phomopsis"
Thomison, P. R. "Factors Affecting the Severity of Phomopsis Seed Decay in Soybeans". In World Soybean Research Conference III: Proceedings, 495–502. New York: CRC Press, 2022. http://dx.doi.org/10.1201/9780429267932-84.
Testo completoPonmurugan, P., e D. Saravanan. "Evaluation of Streptomyces spp. for the Biological Control of Phomopsis Canker Disease of Tea Plants". In Microbiological Research In Agroecosystem Management, 197–206. India: Springer India, 2013. http://dx.doi.org/10.1007/978-81-322-1087-0_14.
Testo completoMoricca, Salvatore, Gloria Innocenti e Alessandro Ragazzi. "Epidemiological Investigations Shed Light on the Ecological Role of the Endophyte Phomopsis quercina in Mediterranean Oak Forests". In Endophytes of Forest Trees, 207–27. Cham: Springer International Publishing, 2018. http://dx.doi.org/10.1007/978-3-319-89833-9_10.
Testo completo"Phoma and Phomopsis". In Molecular Detection of Human Fungal Pathogens. CRC Press, 2011. http://dx.doi.org/10.1201/b11375-18.
Testo completoLi, Shuxian. "Phomopsis Seed Decay of Soybean". In Soybean - Molecular Aspects of Breeding. InTech, 2011. http://dx.doi.org/10.5772/15688.
Testo completoTsantrizos, Youla S. "Bioactive metabolites of the genus Phomopsis". In Structure and chemistry (Part C), 341–59. Elsevier, 1995. http://dx.doi.org/10.1016/s1572-5995(06)80136-5.
Testo completo"Diaporthe/Phomopsis helianthi, the sunflower pathogen: twenty years of research—Jelena Vukojevic",. In Environment and Crop Production, 119–30. CRC Press, 2002. http://dx.doi.org/10.1201/9781482280012-20.
Testo completoNand Tripathi, Atma, Shailesh Kumar Tiwari e Tushar Kanti Behera. "Postharvest Diseases of Vegetable Crops and Their Management". In Postharvest Technology - Recent Advances, New Perspectives and Applications [Working Title]. IntechOpen, 2022. http://dx.doi.org/10.5772/intechopen.101852.
Testo completoAtti di convegni sul tema "Phomopsis"
Zibrov, A. V., e N. A. Bushneva. "THE EFFECT OF THE SOWING DATES ON PLANT AFFECTION WITH SUNFLOWER PHOMOPSIS BLIGHT". In 11-я Всероссийская конференция молодых учёных и специалистов «Актуальные вопросы биологии, селекции, технологии возделывания и переработки сельскохозяйственных культур». V.S. Pustovoit All-Russian Research Institute of Oil Crops, 2021. http://dx.doi.org/10.25230/conf11-2021-174-177.
Testo completoMcGee, Denis C. "Pod Test for Phomopsis Seed Decay of Soybean". In Proceedings of the First Annual Crop Production and Protection Conference. Iowa State University, Digital Press, 1989. http://dx.doi.org/10.31274/icm-180809-301.
Testo completoMathew, Febina M., e Samuel G. Markell. "Insights into the Diaporthe/Phomopsis complex infecting soybeans in the United States". In Proceedings of the 24th Annual Integrated Crop Management Conference. Iowa State University, Digital Press, 2014. http://dx.doi.org/10.31274/icm-180809-153.
Testo completoSoto-Arias, J. P., Alison Robertson, Matthew E. O'Neal e Gary Munkvold. "Virus-Phomopsis interactions on soybean and the effects of insect and disease management practices". In Proceedings of the 28th Annual Integrated Crop Management Conference. Iowa State University, Digital Press, 2010. http://dx.doi.org/10.31274/icm-180809-263.
Testo completoYang, X. B. "Biology and Management of Premature Yellowing and Death of Soybean Caused by Phomopsis Idiaporthe Fungi". In Proceedings of the 13th Annual Integrated Crop Management Conference. Iowa State University, Digital Press, 2001. http://dx.doi.org/10.31274/icm-180809-719.
Testo completoRapporti di organizzazioni sul tema "Phomopsis"
Mueller, Daren, Carl Bradley, Martin Chilvers, Loren Giesler, Febina Mathew, Damon Smith, Kiersten Wise et al. Pod and Stem Blight and Phomopsis Seed Decay. United States: Crop Protection Netework, giugno 2015. http://dx.doi.org/10.31274/cpn-20190620-016.
Testo completoSoto-arias, Jose Pablo, e Gary P. Munkvold. Bean Leaf Beetle-Phomopsis Management Trials on Soybean. Ames: Iowa State University, Digital Repository, 2010. http://dx.doi.org/10.31274/farmprogressreports-180814-420.
Testo completoPetrovic, Kristina, Demetra Skaltsas, Lisa A. Castlebury, Brian Kontz, Tom W. Allen, Martin I. Chilvers, Nancy Gregory et al. New Fungi Discovered Causing Phomopsis Seed Decay of Soybean. Ames (Iowa): Iowa State University. Library, agosto 2021. http://dx.doi.org/10.31274/cpn-20210820-0.
Testo completoPetrovic, Kristina, Demetra Skaltsas, Lisa A. Castlebury, Brian Kontz, Tom W. Allen, Martin I. Chilvers, Nancy Gregory et al. New Fungi Discovered Causing Phomopsis Seed Decay of Soybean. United States of America: Crop Protection Netework, agosto 2021. http://dx.doi.org/10.31274/cpn-20210825-0.
Testo completoPetrovic, Kristina, Demetra Skaltsas, Lisa A. Castlebury, Brian Kontz, Tom W. Allen, Martin I. Chilvers, Nancy Gregory et al. New Fungi Discovered Causing Phomopsis Seed Decay of Soybean. United States of America: Crop Protection Netework, agosto 2021. http://dx.doi.org/10.31274/cpn-20210826-000.
Testo completovan den Top, H. J., e J. G. J. Mol. Determination of phomopsin-A in lupin seeds and lupin-derived products : results of an interlaboratory validation study. Wageningen: RIKILT Wageningen University & Research, 2017. http://dx.doi.org/10.18174/410802.
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