Letteratura scientifica selezionata sul tema "Next generation RNAi and CRISPR for pest management"
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Articoli di riviste sul tema "Next generation RNAi and CRISPR for pest management"
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Belagalla, Ningaraj, Rabinder Kaur e G. J. Abhishek. "Eco-friendly and Targeted through Next-generation Approaches to Insect Pest Management". UTTAR PRADESH JOURNAL OF ZOOLOGY 45, n. 13 (8 giugno 2024): 73–99. http://dx.doi.org/10.56557/upjoz/2024/v45i134137.
Testo completoAbstract (sommario):
Insect pests pose significant challenges to agricultural productivity, human health, and ecosystem stability worldwide. Traditional pest management approaches, heavily reliant on broad-spectrum chemical insecticides, have led to the development of insecticide resistance, unintended effects on non-target organisms, and environmental contamination. In response to these challenges, next-generation approaches to insect pest management are emerging, focusing on eco-friendly and targeted strategies. This review article explores the latest advancements in sustainable pest management, including the use of biopesticides, semiochemicals, biotechnology-based approaches, and integrated pest management (IPM) strategies. Biopesticides, derived from natural sources such as plants, microorganisms, and insects, offer effective and environmentally benign alternatives to synthetic insecticides. Semiochemicals, including pheromones and allelochemicals, can be exploited for pest monitoring, mating disruption, and attract-and-kill strategies. Biotechnology-based approaches, such as RNA interference (RNAi), CRISPR/Cas9 gene editing, and transgenic crops, provide novel tools for targeting specific pest species and reducing reliance on chemical insecticides. IPM strategies, which combine multiple pest management tactics based on ecological principles, offer a holistic and sustainable approach to pest control. The adoption of these next-generation approaches faces challenges, including regulatory hurdles, public acceptance, and the need for further research and development. However, by embracing eco-friendly and targeted pest management strategies, we can reduce the environmental impact of insect pest control, promote biodiversity conservation, and ensure sustainable food production for a growing global population. Future research should focus on optimizing and integrating these approaches, understanding their long-term ecological impacts, and developing effective knowledge transfer mechanisms to promote their widespread adoption.
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SUZUKI, TAKESHI, YUKA ARAI, NAOKI TAKEDA, FATEN ABDELSALAM HAMDI e NOURELDIN ABUELFADL GHAZY. "Towards RNAi-mediated pest mite management: Ingestion, cellular uptake, and intracellular processing of long dsRNAs". Zoosymposia 22 (30 novembre 2022): 46. http://dx.doi.org/10.11646/zoosymposia.22.1.16.
Testo completoAbstract (sommario):
Double-stranded RNA (dsRNA) as a trigger of RNAi-mediated sequence-specific gene silencing is a promising next-generation pesticide that could enable selective pest control ultimately at the species level. The discovery that orally administrated dsRNA induces RNAi in the two-spotted mite, Tetranychus urticae Koch (Trombidiformes: Tetranychidae), has led to a dramatic increase in research toward the development of RNAi-based biopesticides for spider mites.
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Devi, B. Megala, Samyuktha Guruprasath, Pooraniammal Balu, Anirudha Chattopadhyay, Siva Sudha Thilagar, Kanaga Vijayan Dhanabalan, Manoj Choudhary, Swarnalatha Moparthi e A. Abdul Kader Jailani. "Dissecting Diagnostic and Management Strategies for Plant Viral Diseases: What Next?" Agriculture 14, n. 2 (9 febbraio 2024): 284. http://dx.doi.org/10.3390/agriculture14020284.
Testo completoAbstract (sommario):
Recent advancements in molecular biology have revolutionized plant disease diagnosis and management. This review focuses on disease diagnosis through serological techniques, isothermal amplification methods, CRISPR-based approaches, and management strategies using RNA-based methods. Exploring high-throughput sequencing and RNA interference (RNAi) technologies like host-induced gene silencing (HIGS) and spray-induced gene silencing (SIGS), this review delves into their potential. Despite the precision offered by RNAi in pest and pathogen management, challenges such as off-target effects and efficient dsRNA delivery persist. This review discusses the significance of these strategies in preventing aphid-mediated plant virus transmission, emphasizing the crucial role of meticulous dsRNA design for effective viral RNA targeting while minimizing harm to plant RNA. Despite acknowledged challenges, including off-target effects and delivery issues, this review underscores the transformative potential of RNA-based strategies in agriculture. Envisaging reduced pesticide dependency and enhanced productivity, these strategies stand as key players in the future of sustainable agriculture.
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Yoon, June-Sun, Seung-Joon Ahn e Man-Yeon Choi. "Selection and Comparative Gene Expression of Midgut-Specific Targets for Drosophila suzukii". Insects 14, n. 1 (12 gennaio 2023): 76. http://dx.doi.org/10.3390/insects14010076.
Testo completoAbstract (sommario):
Spotted-wing drosophila (SWD), Drosophila suzukii, is a destructive and invasive pest that attacks most small fruits and cherries. The current management for SWD involves the use of conventional insecticides. In an effort to develop a biologically based control option, the application of RNA interference (RNAi) has been investigated. To develop an RNAi approach, suitable targets must be identified, and an efficient delivery method must be developed for introducing the double-stranded RNA (dsRNA) in the midgut. In D. suzukii, we previously found that dsRNA nucleases actively degrade dsRNA molecules in the midgut. In this study, we focused on identifying biological targets focused on the midgut membrane. The profile of midgut-specific genes was analyzed and compared with the genes expressed in the whole-body using transcriptome analysis. Differential gene expression analysis revealed that 1921 contigs were upregulated and 1834 contigs were downregulated in the midgut when compared to genes from other body tissues. We chose ten midgut-specifically upregulated genes and empirically confirmed their expressions. We are particularly interested in the midgut membrane proteins, including G protein-coupled receptors (GPCRs) such as diuretic hormone 31 (DH31) receptor, neuropeptide F (NPF) recepror, toll-9, adhesion receptors, methuselah (mth), and gustatory receptor, because insect GPCRs have been offered great potential for next-generation pest management.
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Singh, Swati, Ashoka, P, Umang Ahlawat, Walunjkar Babasaheb Changdeo, Rizwana Rehsawla, Anu Naruka e Deepika Sharma. "Mechanisms and Applications of Microbial Biotechnology in Soil Health and Agricultural Productivity: A Review". Journal of Advances in Biology & Biotechnology 27, n. 7 (6 luglio 2024): 1420–38. http://dx.doi.org/10.9734/jabb/2024/v27i71104.
Testo completoAbstract (sommario):
Microbial biotechnology in agriculture offers considerable potential for enhancing soil health, increasing crop yields, and promoting sustainability through the application of biofertilizers, biopesticides, and bioremediation techniques. Emerging technologies such as next-generation sequencing, synthetic biology, CRISPR-Cas9, and nanotechnology are driving innovations that improve the efficacy and stability of microbial products. Next-generation sequencing and metagenomics allow for a comprehensive understanding of soil microbial communities, enabling the development of targeted inoculants. Synthetic biology and CRISPR-Cas9 facilitate the engineering of microorganisms with enhanced traits, while nanotechnology and microbial encapsulation improve the delivery and viability of these products. Despite these advancements, the field faces significant challenges including the variability of soil ecosystems, high production costs, limited market availability, and complex regulatory frameworks. Addressing these issues requires targeted research to understand microbial interactions, optimize formulations, and assess environmental impacts. Supportive policies and harmonized regulatory frameworks are essential to streamline approval processes and ensure safety and efficacy. Financial incentives, robust extension services, and public-private partnerships are critical to foster innovation and adoption. Integrating microbial biotechnology with sustainable agricultural practices such as integrated nutrient management (INM) and integrated pest management (IPM) can maximize productivity and environmental benefits. By overcoming these challenges and leveraging advanced technologies, microbial biotechnology can play a pivotal role in achieving sustainable agriculture, ensuring food security, and maintaining environmental health for future generations.
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Kadoić Balaško, Martina, Katarina M. Mikac, Renata Bažok e Darija Lemic. "Modern Techniques in Colorado Potato Beetle (Leptinotarsa decemlineata Say) Control and Resistance Management: History Review and Future Perspectives". Insects 11, n. 9 (1 settembre 2020): 581. http://dx.doi.org/10.3390/insects11090581.
Testo completoAbstract (sommario):
Colorado potato beetle, CPB (Leptinotarsa decemlineata Say), is one of the most important pests of the potato globally. Larvae and adults can cause complete defoliation of potato plant leaves and can lead to a large yield loss. The insect has been successfully suppressed by insecticides; however, over time, has developed resistance to insecticides from various chemical groups, and its once successful control has diminished. The number of available active chemical control substances is decreasing with the process of testing, and registering new products on the market are time-consuming and expensive, with the possibility of resistance ever present. All of these concerns have led to the search for new methods to control CPB and efficient tools to assist with the detection of resistant variants and monitoring of resistant populations. Current strategies that may aid in slowing resistance include gene silencing by RNA interference (RNAi). RNAi, besides providing an efficient tool for gene functional studies, represents a safe, efficient, and eco-friendly strategy for CPB control. Genetically modified (GM) crops that produce the toxins of Bacillus thuringiensis (Bt) have many advantages over agro-technical, mechanical, biological, and chemical measures. However, pest resistance that may occur and public acceptance of GM modified food crops are the main problems associated with Bt crops. Recent developments in the speed, cost, and accuracy of next generation sequencing are revolutionizing the discovery of single nucleotide polymorphisms (SNPs) and field of population genomics. There is a need for effective resistance monitoring programs that are capable of the early detection of resistance and successful implementation of integrated resistance management (IRM). The main focus of this review is on new technologies for CPB control (RNAi) and tools (SNPs) for detection of resistant CPB populations.
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Ahlawat, Umang, Anu Naruka, Walunjkar Babasaheb Changdeo, Rizwana Rehsawla, Rekha Sansanwal, Rishabh Mishra, Kiran e Vivek Kumar Singh. "A Review of Cutting-edge Biotechnological Solutions for Next-generation Farming". Journal of Experimental Agriculture International 46, n. 7 (12 luglio 2024): 1187–204. http://dx.doi.org/10.9734/jeai/2024/v46i72671.
Testo completoAbstract (sommario):
The emerging trends in agricultural biotechnology focus on synthetic biology, gene drives, advanced breeding techniques, and the integration of AI and machine learning. Synthetic biology offers innovative solutions by designing new biological systems and reengineering existing ones, enhancing traits such as drought tolerance and nutrient biosynthesis in crops. Gene drives and advanced breeding techniques, particularly CRISPR-Cas9, enable precise genetic modifications to control pest populations and improve crop resilience. AI and machine learning revolutionize agriculture through predictive analytics with real-time decision-making, optimizing resource use, and increasing efficiency. These technologies provide actionable insights into crop management, from detecting stress factors to predicting yields. The review also highlights the importance of international collaborations and global initiatives, such as CGIAR and GACSA, which play a critical role in sharing knowledge, resources, and biotechnological advancements to address food security and sustainability. Capacity-building programs and technology transfer initiatives, exemplified by AGRA, enhance technological capabilities in developing regions. Global efforts, like the FAO's Sustainable Food and Agriculture program and the ITPGRFA, emphasize the conservation and sustainable use of genetic resources, supporting the development of resilient crop varieties. Despite the promising benefits, challenges such as regulatory hurdles, public perception, and ethical considerations must be addressed to ensure the responsible adoption of these technologies. By fostering interdisciplinary research, enhancing public education, and promoting sustainable farming practices, these biotechnological innovations can be harnessed to create a resilient and sustainable agricultural system, ultimately contributing to global food security and environmental health. This analysis underscores the transformative potential of biotechnology in shaping the future of agriculture.
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Nitnavare, Rahul B., Joorie Bhattacharya, Satnam Singh, Amardeep Kour, Malcolm J. Hawkesford e Naveen Arora. "Next Generation dsRNA-Based Insect Control: Success So Far and Challenges". Frontiers in Plant Science 12 (18 ottobre 2021). http://dx.doi.org/10.3389/fpls.2021.673576.
Testo completoAbstract (sommario):
RNA interference (RNAi) is a method of gene silencing where dsRNA is digested into small interfering RNA (siRNA) in the presence of enzymes. These siRNAs then target homologous mRNA sequences aided by the RNA-induced silencing complex (RISC). The mechanism of dsRNA uptake has been well studied and established across many living organisms including insects. In insects, RNAi is a novel and potential tool to develop future pest management means targeting various classes of insects including dipterans, coleopterans, hemipterans, lepidopterans, hymenopterans and isopterans. However, the extent of RNAi in individual class varies due to underlying mechanisms. The present review focuses on three major insect classes viz hemipterans, lepidopterans and coleopterans and the rationale behind this lies in the fact that studies pertaining to RNAi has been extensively performed in these groups. Additionally, these classes harbour major agriculturally important pest species which require due attention. Interestingly, all the three classes exhibit varying levels of RNAi efficiencies with the coleopterans exhibiting maximum response, while hemipterans are relatively inefficient. Lepidopterans on the other hand, show minimum response to RNAi. This has been attributed to many facts and few important being endosomal escape, high activity dsRNA-specific nucleases, and highly alkaline gut environment which renders the dsRNA unstable. Various methods have been established to ensure safe delivery of dsRNA into the biological system of the insect. The most common method for dsRNA administration is supplementing the diet of insects via spraying onto leaves and other commonly eaten parts of the plant. This method is environment-friendly and superior to the hazardous effects of pesticides. Another method involves submergence of root systems in dsRNA solutions and subsequent uptake by the phloem. Additionally, more recent techniques are nanoparticle- and Agrobacterium-mediated delivery systems. However, due to the novelty of these biotechnological methods and recalcitrant nature of certain crops, further optimization is required. This review emphasizes on RNAi developments in agriculturally important insect species and the major hurdles for efficient RNAi in these groups. The review also discusses in detail the development of new techniques to enhance RNAi efficiency using liposomes and nanoparticles, transplastomics, microbial-mediated delivery and chemical methods.
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McGruddy, Rose A., Zoe E. Smeele, Brian Manley, James D. Masucci, John Haywood e Philip J. Lester. "RNA interference as a next‐generation control method for suppressing Varroa destructor reproduction in honey bee (Apis mellifera) hives". Pest Management Science, 27 maggio 2024. http://dx.doi.org/10.1002/ps.8193.
Testo completoAbstract (sommario):
AbstractBACKGROUNDThe Varroa mite (Varroa destructor) is considered to be the greatest threat to apiculture worldwide. RNA interference (RNAi) using double‐stranded RNA (dsRNA) as a gene silencing mechanism has emerged as a next‐generation strategy for mite control.RESULTSWe explored the impact of a dsRNA biopesticide, named vadescana, designed to silence the calmodulin gene in Varroa, on mite fitness in mini‐hives housed in a laboratory. Two dosages were tested: 2 g/L dsRNA and 8 g/L dsRNA. Vadescana appeared to have no effect on mite survival, however, mite fertility was substantially reduced. The majority of foundress mites exposed to vadescana failed to produce any offspring. No dose‐dependent effect of vadescana was observed, as both the low and high doses inhibited mite reproduction equally well in the mini‐hives and neither dose impacted pupal survival of the honey bee. Approximately 95% of bee pupae were alive at uncapping across all treatment groups.CONCLUSIONThese findings suggest that vadescana has significant potential as an effective alternative to conventional methods for Varroa control, with broader implications for the utilization of RNAi as a next‐generation tool in the management of pest species. © 2024 The Author(s). Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
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Knorr, Eileen, Andre Billion, Elane Fishilevich, Linda Tenbusch, Meghan L. F. Frey, Murugesan Rangasamy, Premchand Gandra et al. "Knockdown of Genes Involved in Transcription and Splicing Reveals Novel RNAi Targets for Pest Control". Frontiers in Agronomy 3 (3 settembre 2021). http://dx.doi.org/10.3389/fagro.2021.715823.
Testo completoAbstract (sommario):
RNA interference (RNAi) is a promising next generation technology for the development of species-specific pest management. The key to successful RNAi based-plant protection is dependent in part on data-driven target gene selection, a challenging task due to the absence of laboratory strains and the seasonality of most pest species. In this study, we aimed to identify novel target genes by performing a knowledge-based approach in order to expand the spectrum of known potent RNAi targets. Recently, the protein-coding genes ncm, Rop, RPII-140, and dre4 have been identified as sensitive RNAi targets for pest control. Based on these potent RNAi targets, we constructed an interaction network and analyzed a selection of 30 genes in the model beetle Tribolium castaneum via injection of dsRNA synthesized by in vitro transcription. Nineteen of these targets induced significant mortality of over 70%, including six that caused 100% lethality. Orthologs of active T. castaneum RNAi targets were verified in the economically important coleopteran pests Diabrotica virgifera virgifera and Brassicogethes aeneus. Knockdown of D. v. virgifera genes coding for transcription factor Spt5, Spt6, and RNA polymerase II subunit RPII-33 caused over 90% mortality in larval feeding assays. Injection of dsRNA constructs targeting RPII-215 or the pre-mRNA-processing factor Prp19 into adult B. aeneus resulted in high lethality rates of 93 and 87%, respectively. In summary, the demonstrated knowledge-based approaches increased the probability of identifying novel lethal RNAi target genes from 2% (whole genome) to 36% (transcription- and splicing-related genes). In addition, performing RNAi pre-screening in a model insect increased also the probability of the identification essential genes in the difficult-to-work-with pest species D. v. virgifera and B. aeneus.
Libri sul tema "Next generation RNAi and CRISPR for pest management"
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Pathak, Dharminder, Satnam Singh, Harish Kumar, Gomti Grover e Navneet Kaur, a cura di. Cotton Some: Insights. The Crop Improvement Society of India, 2023.
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