Letteratura scientifica selezionata sul tema "Nano-Antibody"
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Articoli di riviste sul tema "Nano-Antibody":
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Peng, Jianyun, Yongling Tao, Xiaoru Zhang, Meijuan Xiang, Zhihong Gui, Jing Wu e Liqing Yin. "Effect of Preparation of β2M Nano Antibody Adsorbent for Blood Purification on Dialysis Complications in Patients with Renal Failure". Science of Advanced Materials 13, n. 1 (1 gennaio 2021): 161–70. http://dx.doi.org/10.1166/sam.2021.3881.
Abstract (sommario):
In order to purify blood and adsorb β2M, a highly selective immunosorbent biomaterial based on nano antibody was designed. First of all, nanoantibodies against β2M in patients with renal failure are screened by phage display library technology. After 4 rounds of screening, 12 kinds of monoclonal phage samples are compared by ELISA, and the highest affinity sequence A53 is obtained. Then, the nano antibody A53 is transferred into two kinds of host bacteria respectively, and the host E. coli Shuffle T7 is selected, and then purified by nickel column affinity, thus obtaining high purity nano antibody A53S. Five kinds of nano antibody adsorbents are prepared by using A53S as a component of adsorbent. The results show that the effect of D series adsorbent with ɛ-poly-L-lysine as spacer is better, but the ligand density should be adjusted reasonably to ensure the activity of nanoantibody protein. When the loading concentration of β2M is set to 1.8 mg/ml, adsorbent D-1 can adsorb 11.78 mg/ml of dynamic adsorption capacity of β2M. This index is significantly higher than the dynamic adsorption capacity of plasma system under the same conditions (P < 0.05), which proves that the nano antibody adsorbent biomaterial can effectively adsorb β2M, thus reducing the probability of dialysis complications.
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Wang, Yi, Yujin Feng, Xiaoyun Yang, Wengang Wang e Yueheng Wang. "Diagnosis of Atherosclerotic Plaques Using Vascular Endothelial Growth Factor Receptor-2 Targeting Antibody Nano-microbubble as Ultrasound Contrast Agent". Computational and Mathematical Methods in Medicine 2022 (5 maggio 2022): 1–7. http://dx.doi.org/10.1155/2022/6524592.
Abstract (sommario):
The atherosclerotic plaque is characterized by narrowing of blood vessels and reduced blood flow leading to the insufficient blood supply to the brain. The hemodynamic changes caused by arterial stenosis increase the shearing force of the fibrous cap on the surface of the plaque, thereby reducing the stability of the plaque. Unstable plaques are more likely to promote angiogenesis and increase the risk of patients with cerebrovascular diseases. A timely understanding of the formation and stability of the arterial plaque can guide in taking targeted measures for reducing the risk of acute stroke in patients. It has been confirmed that nano-microbubbles can enter these plaques through the gaps in the patient’s vascular endothelial cells, thereby enhancing the acquisition of ultrasound information for plaque visualization. Therefore, we aim to investigate the diagnostic value of targeted nano-microbubbles for atherosclerotic plaques. This study constructed vascular endothelial growth factor receptor-2 (VEGFR-2) targeting antibody nano-microbubbles and compared its diagnostic value with that of blank nano-microbubbles for atherosclerotic plaques. Studies have found that VEGFR-2 targeting antibody nano-microbubbles can accurately detect the position of plaques. Its detection rate, sensitivity, and specificity for plaques are higher than those of blank nano-microbubbles. Similarly, the peak intensity and average transit time of VEGFR-2 targeting antibody nano-microbubbles were greater than those of blank nano-microbubbles. Therefore, we believe that the combination of VEGFR-2 antibody and nano-microbubbles can enhance the acquisition of ultrasound information on atherosclerotic plaque neovascularization, thereby improving the early diagnosis of unstable plaque.
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Mahmoudi, Tohid, Mohammad Pourhassan-Moghaddam, Behnaz Shirdel, Behzad Baradaran, Eden Morales-Narváez e Hamed Golmohammadi. "(Nano)tag–antibody conjugates in rapid tests". Journal of Materials Chemistry B 9, n. 27 (2021): 5414–38. http://dx.doi.org/10.1039/d1tb00571e.
Abstract (sommario):
Antibodies are naturally derived materials with favorable affinity, selectivity, and fast binding kinetics to the respective antigens, which enables their application as promising recognition elements in the development of various types of rapid tests.
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Gupta, Ankur, Monalisha Nayak, Deepak Singh e Shantanu Bhattacharya. "Antibody immobilization for ZnO nanowire based biosensor application". MRS Proceedings 1675 (2014): 33–39. http://dx.doi.org/10.1557/opl.2014.848.
Abstract (sommario):
ABSTRACTDue to the high surface area and good bio-compatibility of nano structured ZnO, it finds good utility in biosensor applications. In this work we have fabricated highly dense ZnO nano bundles with the assistance of self assembled poly methylsilisesquoxane (PMSSQ) matrix which has been realized in a carpet like configuration with implanted ZnO nano-seeds. Such high aspect ratio structures (∼50) with carpet like layout have been realized for the first time using solution chemistry. Nanoparticles of PMMSQ are mixed with a nano-assembler Poly-propylene glycol (PPG) and Zinc Oxide nanoseeds (5-15 nm). The PPG acts by assembling the PMSSQ nanoparticles and evaporates from this film thus creating the highly porous nano-assembly of PMMSQ nanoparticles with implanted Zinc Oxide seeds. Nano-wire bundles with a high overall surface roughness are grown over this template by a daylong incubation of an aqueous solution of hexamethylene tetra amine and Zinc nitrate. Characterization of the fabricated structures has been extensively performed using FESEM, EDAX, and XRD. We envision these films to have potential of highly dense immobilization platforms for antibodies in immunosensors. The principle advantage in our case is a high aspect ratio of the nano-bundles and a high level of roughness in overall surface topology of the carpet outgrowing the zinc-oxide nanowire bundles. Antibody immobilization has been performed by modifying the surface with protein-G followed by Goat anti salmonella antibody. Antibody activity has been characterized by using 3D profiler, Bio-Rad Protein assay and UV-Visible spectrophotometer.
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Souto, Elizabeth XISTO, Laiz CAMEIRAO Bento, Flavia ARANDAS Sousa, Marilia SANDOVAL Passaro, Andressa DA COSTA Vaz, Daniela Schimidell, Barbara FAZIALI Bueno et al. "Anti-CD38 Nanoantibody (JK36) Allows Detection of Minimal Residual Disease in Multiple Myeloma Patients Treated with Daratumumab". Blood 142, Supplement 1 (28 novembre 2023): 4703. http://dx.doi.org/10.1182/blood-2023-182757.
Abstract (sommario):
Objective: To evaluate the performance of the camel anti-CD38 antibody JK36 by flow cytometry in patients with Multiple Myeloma treated with Daratumumab. Materials and Methods: 7 bone marrow samples from patients with Multiple Myeloma treated with Daratumumab were evaluated. The immunophenotypic markers studied routinely were: Tube 1 CD38 FITC (T16 - Beckman Coulter), CD56 PE, CD20 PERCP-Cy5.5, CD19 PC7, Kappacy APC, Lambdacy APC-H7, CD45 V450 and CD138 V500; Tube 2 CD38 FITC (T16 - Beckman Coulter), CD28 PE, CD27 PERCP-Cy5.5, CD19 PC7, CD117 APC, CD81 APC-H7, CD45 V450 AND CD138 V500. For the detection of CD38 depletion, a Nano Tube was made with the following markers Kappacy FITC, CD56 PE, CD20 PERCP-Cy5.5, CD19 PC7, CD38 APC (nano antibody from camel JK36 - Beckman Coulter), Lambdacy APC-H7, CD45 V450 and CD138 V500. The samples were analyzed by the immunophenotyping method by flow cytometry, using the CANTO II equipment (Becton Dickinson - BD) and the analysis was performed in the Infinicyt software (Cytognos). The maximum total events acquired was 3,400,000 with merge between tubes 1 and 2. For Tube Nano, the maximum total events acquired was 1,700,000. Results: In the routinely used panel (Tube 1 and Tube 2) CD38 expression in plasma cells was negative in all samples. However, in all samples, it was possible to identify plasmocytes through expression of CD138 in conjunction with multiple gate strategies that include expressions of aberrant markers. In the Nano Tube containing the marker CD38 Nano Antibody of Camel (JK36) it was possible to visualize the expression of CD38 in the plasmocytes of all analyzed samples. Furthermore, with this marker it was possible to discriminate abnormal plasma cells from normal ones. Discussion: The expressions of CD38 together with CD138 characterize plasma cells. Anti-CD38 targeted therapy (Daratumumab) induces immune-mediated clearance of cells expressing CD38. The detection of plasma cells by flow cytometry with the use of this conventional antibody on the cell surface is impaired after the use of Daratumumab. Nano antibodies that occur naturally in camelids are capable of recognizing epitopes hidden and not blocked by targeted therapies, allowing the detection of plasma cells in patients with Multiple Myeloma after this treatment. In our study, we demonstrated that the Camel Nano Antibody CD38 antibody (JK36) was able to detect plasma cells in patients after using Daratumumab. Although it was possible to identify plasmocytes through the expression of CD138, this strategy becomes very risky and difficult to apply since the expression of this marker can often be of low intensity. The nanoantibody proved to be an additional and useful marker in the detection of MRD in patients after anti-CD38 target therapy (Dartumumab), bringing greater reliability in the analysis. Conclusion: The Camel Nano Antibody CD38 antibody (JK36) offers flow cytometry laboratories the opportunity to more accurately monitor response to anti-CD38 therapies. Nano antibody technology enhances the ability of DRM detection by flow cytometry in the era of immunotherapy.
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Puertas, S., M. Moros, R. Fernández-Pacheco, M. R. Ibarra, V. Grazú e J. M. de la Fuente. "Designing novel nano-immunoassays: antibody orientation versus sensitivity". Journal of Physics D: Applied Physics 43, n. 47 (11 novembre 2010): 474012. http://dx.doi.org/10.1088/0022-3727/43/47/474012.
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Kumar D. R, Santhosh, e Dr P.V. Rao. "HPV Sensing by CNTFET Array Nanobiosensor." International Journal of Engineering & Technology 7, n. 3.34 (1 settembre 2018): 82. http://dx.doi.org/10.14419/ijet.v7i3.34.18778.
Abstract (sommario):
Women life is threatened by the cervical cancer each time worldwide. The cervical cancer high grade pre cancer is due to HPV E6 and E7 agents. Nano materials show a key role in medical analysis and action. CNTs (Carbon Nano Tube) have very unique electrical and mechanical properties which are useful in the bio-application. The conventional based biosensors can be improved by CNT based biosensors with respect to sensitivity, selectivity and simple in operation. In comparison with the silicon transistors, CNTFET (Carbon Nano Tube Field Effect Transistor) nano device takes less power and performs faster. The research paper covers working of CNTFET based nano biosensor to detect cervical cancer antibody. 4 x 4 CNTFET sensor array is designed to detect antibody variations on CNTFET gate. The sensor current varied from 4.286 µA to 15.435 µA for gate voltage varied from 0.2 V to 1.06 V. The improved 64 CNTFET based biosensor performs better in sensing the analyte of different concentrations.
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Cao, Fei, Qian Yao, Tieshan Yang, Zhao Zhang, Yu Han, Jinchao Feng e Xiu-Hong Wang. "Marriage of antibody–drug conjugate with gold nanorods to achieve multi-modal ablation of breast cancer cells and enhanced photoacoustic performance". RSC Advances 6, n. 52 (2016): 46594–606. http://dx.doi.org/10.1039/c6ra01557c.
Abstract (sommario):
A multifunctional nano platform against cancer using SiO2-coated gold nanorods and antibody–drug conjugate is constructed. It incorporates active targeting, antibody therapy, drug therapy, photothermal therapy, and enhanced photoacoustic performance.
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Schneider, Constantin, Matthew I. J. Raybould e Charlotte M. Deane. "SAbDab in the age of biotherapeutics: updates including SAbDab-nano, the nanobody structure tracker". Nucleic Acids Research 50, n. D1 (19 novembre 2021): D1368—D1372. http://dx.doi.org/10.1093/nar/gkab1050.
Abstract (sommario):
Abstract In 2013, we released the Structural Antibody Database (SAbDab), a publicly available repository of experimentally determined antibody structures. In the interim, the rapid increase in the number of antibody structure depositions to the Protein Data Bank, driven primarily by increased interest in antibodies as biotherapeutics, has led us to implement several improvements to the original database infrastructure. These include the development of SAbDab-nano, a sub-database that tracks nanobodies (heavy chain-only antibodies) which have seen a particular growth in attention from both the academic and pharmaceutical research communities over the past few years. Both SAbDab and SAbDab-nano are updated weekly, comprehensively annotated with the latest features described here, and are freely accessible at opig.stats.ox.ac.uk/webapps/newsabdab/.
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Buyukserin, Fatih, Colin D. Medley, Miguel O. Mota, Kaan Kececi, Richard R. Rogers, Weihong Tan e Charles R. Martin. "Antibody-functionalized nano test tubes target breast cancer cells". Nanomedicine 3, n. 3 (giugno 2008): 283–92. http://dx.doi.org/10.2217/17435889.3.3.283.
Tesi sul tema "Nano-Antibody":
1
Hmaidi, Riadh. "Nouvelle stratégie basée sur les polypeptides comme agents modulateurs des processus tumoraux". Electronic Thesis or Diss., Amiens, 2022. http://www.theses.fr/2022AMIE0073.
Abstract (sommario):
Les produits d'origine naturelle sont de plus en plus recherchés et testés pour leur potentiel thérapeutique. Les peptides issus de venins de scorpions capables de moduler l'activité des canaux ioniques constituent de robustes outils biologiques essentiels pour une meilleure compréhension des mécanismes de fonctionnement de ces canaux transmembranaires, sélectifs notamment au sodium. Ils représentent parfois les molécules majeures du venin responsables de son effet hautement toxique qui se manifeste par des syndromes cardiogéniques et d'un œdème pulmonaire souvent fatal pour l'Homme. En modulant l'activité des canaux ioniques ces molécules sont potentiellement capables d'agir sur les processus cellulaires qui leur sont associés. Dans une première partie de ce travail, nous nous sommes intéressés au mécanisme d'interaction de la toxine AahII du venin du scorpion Androctonus australis hector (Aah) avec le canal sodique voltage-dépendant NaV1.5 et de son effet sur l'activité électrophysiologique de ce canal. Nous avons en particulier étudié la capacité du nanoanticorps anti-AahII (NbAahII 10 ou Nb10) à neutraliser l'interaction AahII/NaV1.5. Dans une seconde partie, nous avons investigué l'effet de différentes fractions peptidiques issues du venin de Aah sur la viabilité des cellules cancéreuses mammaires humaines. La toxine AahII est considérée comme l'α-toxine la plus active du venin de scorpion Aah. Elle a notamment été décrite pour ralentir l'inactivation des canaux sodiques NaV. Le nanoanticorps Nb10 a été développé afin de neutraliser l'effet de la toxine AahII. L'efficacité du Nb10 a été évaluée in-vivo sur des souris, mais son mécanisme d'action au niveau cellulaire reste inconnu. Nos travaux ont confirmé que AahII ralentit de manière dose dépendante l'inactivation des canaux sodiques NaV1.5 surexprimés dans des cellules HEK293, sans modifier l'amplitude du courant au pic d'une part et ont montré que le Nb10 neutralise l'effet de la toxine AahII sur la cinétique d'inactivation du canal d'autre part. L'analyse bioinformatique du complexe d'interaction de NaV1.5/AahII montre que la AahII partage la même surface d'interaction avec le Nb10, ce qui suggère fortement que ce dernier déplace dynamiquement la toxine AahII de son site de liaison sur le canal NaV1.5. Au niveau physiopathologique, nous avons montré que le traitement des cellules cancéreuses mammaires humaines MDA-MB-231 avec le Nb10 prévient l'augmentation de l'invasion cellulaire induite par AahII. Dans la seconde partie de ce travail, les 3 majeures fractions issues du venin de scorpion Aah, préalablement isolées par chromatographie à basse pression (M1, M2 et AahG50), ont été testées sur la viabilité et la migration des lignées cellulaires cancéreuses mammaires humaines, MCF-7 et MDA-MB-231. Ces fractions n'ont pas eu d'effet sur la migration cellulaire mais ont induit des effets très biphasiques sur la viabilité cellulaire, démontrant de manière intéressante des effets synergiques ou antagonistes des peptides qui les composent. Afin de purifier les peptides d'intérêts, une purification supplémentaire des fractions a été réalisée par chromatographie à haute pression (HPLC). Au total, trois polypeptides ont été identifiées pour induire une diminution de la viabilité des cellules cancéreuses mammaires et feront l'objet d'investigation plus poussée. En conclusion, nos résultats ont permis d'élucider les mécanismes physiologiques et moléculaires de neutralisation de l'α-toxine AahII par le nanoanticorps Nb10. De plus, nous avons pu identifier trois molécules peptidiques issues du venin de scorpion Aah possédant des propriétés anticancéreuses. Ces travaux se poursuivront par une étude structurale permettant d'élucider à une échelle tridimensionnelle, les topologies moléculaires sous-jacentesNatural molecules are increasingly being researched and tested for their therapeutic potential. Scorpion venoms derived peptides modulating ion channels activity are robust biological essential tools for a better understanding of the action mechanisms of these transmembrane channels, particularly sodium channels. They represent sometimes the major molecules of the venom responsible for its highly toxic effect which manifests with cardiogenic syndromes and pulmonary edema often fatal for humans. By modulating ion channels activity, these molecules are potentially able to act on the cellular processes associated with them. In the first part of this work, we have studied the mechanism of interaction of the AahII toxin from the scorpion venom of Androctonus australis hector (Aah), with the voltage-gated sodium channel NaV1.5 and its effect on the electrophysiological activity of this channel. In particular, we studied the ability of the anti-AahII nanoantibody (NbAahII 10 or Nb10) to neutralize the AahII/NaV1.5 interactions. In the second part, we investigated the effect of different peptide fractions from Aah venom on the viability of human breast cancer cells. The toxin AahII is the most active α-toxin from the North African scorpion Androctonus australis hector that slows the fast inactivation of NaV channels. To fight scorpion envenomation, an anti-AahII nanobody named NbAahII10 (Nb10) was developed. The efficiency of this nanobody has been evaluated in vivo on mice, but its mechanism of action at the cellular level remains unknown. Our work confirmed that the AahII toxin slows the fast inactivation of the adult cardiac NaV1.5 channels, expressed in HEK293 cells, in a dose-dependent manner, while current amplitude was not affected, and showed that Nb10 can fully reverse the effect of the AahII toxin on the channel inactivation kinetics. Bioinformatic analysis of the NaV1.5/AahII interaction complex shows that AahII shares the same interaction surface with Nb10, which strongly suggests that Nb10 dynamically replaces the AahII toxin from its binding site on the NaV1.5 channel. At the pathophysiological level, we showed that treatment of human breast cancer cells MDA-MB-231 with Nb10 prevents the increase in cell invasion induced by AahII. In the second part of this work, the 3 major fractions from Aah scorpion venom, previously isolated by low-pressure chromatography (M1, M2, and AahG50), were tested on the viability and migration of human breast cancer cell lines, MCF-7 and MDA-MB-231. These fractions did not affect cell migration but induced very biphasic effects on cell viability, interestingly demonstrating synergistic or antagonistic effects of their component peptides. To purify the peptides of interest, additional purification of the fractions was performed by high-pressure chromatography (HPLC). In total, three polypeptides were identified to induce a decrease in breast cancer cell viability and will be further investigated. In conclusion, our results elucidated the physiological and molecular mechanisms of α-toxin AahII neutralization by the Nb10 nanoantibody. In addition, we were able to identify three peptide molecules from the scorpion venom Aah with anticancer properties. This work will continue with a structural study to elucidate the underlying molecular topologies at a three-dimensional scale
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SIRONI, LAURA. "Nanoparticles for in-vitro and in-vivo biosensing and imaging". Doctoral thesis, Università degli Studi di Milano-Bicocca, 2011. http://hdl.handle.net/10281/19278.
Abstract (sommario):
In the last two decades several groups have investigated the changes of chemical and physical properties of materials with size in nanometric scales. These studies have highlighted a number of possible applications for nanostructures, which are now employed, for example, in biology and medicine for imaging, disease detection, diagnosis, sensing and therapy.
Noble metals (especially gold and silver nanoparticles) are particularly versatile for these applications due to the phenomenon known as surface plasmon resonance (SPR), an in-phase oscillations of all the conduction band electrons that resonates with the light wave electric field. The resonance frequency depends on the size, shape, orientation and dielectric constant of the nanoparticle. This coupling of SPR with the electromagnetic field leads to a large enhancement of all the nanoparticle radiative properties, such as absorption and scattering. The extinction cross section of these nanoparticles is 10^5-10^6 larger than that of organic dyes and in contrast to common molecular chromophores they are extremely photostable and, depending on the shape, they convert efficiently light into heat. The SPR, tunable in the visible (for spherical NPs) and near-infrared region (for anisotropic Nps) of the electromagnetic spectrum, can also interact, for gold nanoparticles a few nanometers in size, with the fluorescence emission of dyes and substantially modify their brightness and excited-state lifetime. Depending on the fluorophores-NP distance and the NPs anisotropy one can obtain fluorescence enhancement or quenching. In both cases we expect that any change in the dielectric constant of the NP surface, induced, for example, by a biorecognition process that occurs on the surface itself, can produce a change in the emission properties of the fluorophores.
SPR effect becomes also particularly important when combined with two-photon excitation (TPE), which consists in the simultaneous absorption of two photons, each carrying about half the energy necessary to excite the molecule. TPE offers a series of unique features for biological investigation both in vitro and in vivo. First, the two-photon absorption bands of the dyes commonly used in biological studies are wider than their one-photon analogous allowing the simultaneous excitation of multiple fluorophores with a single excitation wavelength. Second, the stimulating light beam has a high penetration depth because of the long infrared wavelengths used, allowing experiments in turbid media. Third, excitation takes place only at the plane of focus, due to the scaling of the probability of simultaneous photon absorption with the square of the light intensity. As a consequence TPE avoids the simultaneous absorption of photons outside the specimen drastically reducing both photo-toxicity and fluorophore bleaching. These advantages make nowadays TPE a well established tool for scientific biological and medical research and can be coupled to anisotropic gold nanoparticles.
In fact, the luminescence (TPL) induced by TPE is enhanced (when coupled with an appropriate plasmon resonance) by many orders of magnitude in non spherically symmetric NPs of noble metal with respect to the single photon excitation on smooth noble metal surfaces. These properties promise to improve the usefulness of these nanoparticles for in-vivo imaging in the NIR region of the electromagnetic spectrum. According to these considerations we have developed our project on two lines related to the use of gold nanoparticles for sensing and non linear imaging. The aim of the first part of this research project is to exploit changes of the dye excited-state lifetime and brightness induced by its interaction with the gold surface plasmons for detection of tiny amounts of protein in solution under physiological conditions. The system we investigated is based on 10 and 5 nm diameter gold NPs coupled (via a biotin- streptavidin linker) to the FITC dye and to a specific protein antibody. The interaction of the fluorophore with the gold surface plasmon resonances, mainly occurring through quenching, affects the excited state lifetime that is measured by fluorescence burst analysis in highly diluted suspensions. The binding of protein to the gold NPs through antigen-antibody recognition further modifies the dye excited-state lifetime, which change can then be used to measure the protein concentration. In particular, we have tested the nanodevice measuring the change of the fluorophore excited-state lifetime after the binding of the model protein bovine serum albumin (BSA); then we have applied the nanoassay in order to recognize the p53 protein, whose detection in the body is highly valuable as marker for early cancer diagnosis and prognosis, both in standard solutions and in total cell extracts. The selectivity of the construct with respect other globular proteins has been also addressed. The data indicate that the FITC excited-state lifetime is a very sensitive parameter in order to detect tiny amounts of protein in solution with an estimated limit of detection of about 5 pM, mostly determined by the statistical accuracy of the lifetime measurement.
In the second part of the project we focused on the exploitation of anisotropic gold nanoparticles as probes in cellular imaging. We have then studied their photoluminescence (TPL) properties under two photon excitation. We have focused on gold nanorods that can easily be obtained by synthesis with the standard surfactant CTAB (cetyl trimethylammonium bromide). The synthesis of asymmetric branched gold nanoparticles, obtained using for the first time in the seed growth method approach a zwitterionic surfactant, laurylsulphobetaine (LSB), has been developed in collaboration with the University of Pavia (Prof. P.Pallavicini). We have shown that LSB concentration in the growth solution allows to control the dimension of the NPs and the SPR position, that can be tuned in the 700-1100 nm Near Infrared range. The samples have been analyzed with a number of structural techniques to obtain a complete characterization: absorption spectra in the UV-Visible region, TEM images of the suspensions, Fluorescence Correlation Spectroscopy (FCS) and Dynamic Light Scattering (DLS) experiments in suspensions. From these data we reached information on the nanoparticles shapes, dimensions and aggregation. In particular, three different populations have been found: nanospheres with diameter lower than 20 nm, nanostars characterized by large trapezoidal branches, and asymmetric branched nanoparticles with high aspect ratio (3-4). A full characterization of the NPs TPL was performed for imaging applications by employing two photon excitation (TPE). The dependence of the TPL intensity on the power, wavelength and polarization of the incident light intensity was studied and TPL was exploited to study the cellular uptake of the nanoparticles in different cell lines (macrophages and HEK cells).
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Dréan, Raphaelle. "Développement de nano-anticorps antagonistes du point de contrôle immunitaire ILT4 pour une application en immunothérapie antitumorale". Electronic Thesis or Diss., Sorbonne université, 2022. http://www.theses.fr/2022SORUS446.
Abstract (sommario):
Le récepteur ILT4 est un point de contrôle immunitaire, principalement exprimé par les cellules myéloïdes du système immunitaire. Dans le contexte cancéreux, ILT4 participe au développement tumoral en maintenant un microenvironnement immunitaire protumoral et en activant directement la prolifération tumorale. L’activation d’ILT4 par la molécule du complexe majeur d’histocompatibilité (CMH) de classe I non-classique HLA-G induit des cellules myéloïdes tolérogènes. De plus, l’expression ectopique d’ILT4 a été rapportée dans plusieurs types de cancers. L’interaction entre l’angiopoiétine-like2 (ANGPTL2) et l’ILT4 promeut la prolifération de cellules de cancer du poumon non à petites cellules et inhibe leur apoptose. Cibler ce nouveau point de contrôle immunitaire par des anticorps bloquants est donc une approche prometteuse en immunothérapie. A la lumière de certaines limitations des anticorps conventionnels bloquants en thérapies des tumeurs solides, nous avons investigué le potentiel d’inhibiteurs basés sur des VHH. Ce petit format d’anticorps, dérivé des anticorps homodimériques de camélidés, allie les propriétés de spécificité des anticorps à celles des petites molécules. Grâce au criblage par phage-display d’une banque immunologique obtenue par immunisation d’un alpaga, nous avons sélectionné un VHH hautement affin et spécifique pour ILT4, qui inhibe l’interaction entre le récepteur et ses deux ligands. Nous avons démontré l’effet biologique antagoniste du VHH in vitro sur des modèles de cellules tumorales et de macrophages de type M2 protumoraux issus de monocytes. Ces premiers résultats de caractérisation supportent le développement futur de ce nouveau format d’anticorps VHH bloquant ILT4 en immunothérapie antitumoraleILT4 (Immunoglobulin-Like Transcript 4) is an immune checkpoint receptor mainly expressed by myeloid immune cells. In cancer context, ILT4 participates in tumor development by maintaining a protumoral immuno-microenvironment and directly promoting tumor cell proliferation. ILT4 interaction with the non-classical MCH class I molecule HLA-G induces an immunosuppressive microenvironment by promoting tolerogenic myeloid cells. Moreover, the ectopic expression of ILT4 has been reported in several solid tumors. The activation of ILT4 by Angiopoietin-like-2 (ANGPTL2) promotes non-small cell lung tumor cell proliferation and inhibits cell apoptosis. Targeting this new immune checkpoint with blocking antibodies is therefore a promising cancer immunotherapy approach. In light of several drawbacks of classical IgG blocking antibodies in solid cancer, we investigated the potential of VHH-based inhibitors. This small monoclonal antibody format, derived from camelid homodimeric antibodies, combine the binding capacities of antibodies to the properties of small molecules. After immunization of an alpaca and phage-display screening, we selected a VHH with high affinity and specificity to ILT4 that inhibits the interaction of the receptor with both ligands. We validated the VHH’s biological antagonist activity on tumor cells and monocyte-derived pro-tumoral M2 like macrophages in vitro. These results support the potential of this new VHH-based antibody targeting ILT4 in cancer immunotherapy
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Chen, Chiao-Yu, e 陳喬郁. "Isolation of monoclonal antibody with binding activity specific to bio-nano interface". Thesis, 2015. http://ndltd.ncl.edu.tw/handle/829k33.
Abstract (sommario):
碩士國立交通大學
材料科學與工程學系奈米科技碩博士班
104
There are more and more application of nano-materials in medicine and biotechnology. The interest in nano-systems for biological applications is continuously growing. To explore the potential of nano-material in the application of drug delivery, artificial implants, and bio-electronics, the fundamental rules underlining the bio-nano interaction should be carefully investigated. Understanding the bio-nano interface is the key to develop and better use of bionanotechnology. I have demonstrated several specific bio-nano interface previously. I also showed that antibody can recognize gold nanoparticles. Our approach is to develop monoclonal antibody against gold nanoparticles which will serve as the base for further biophysical study and applications. The result indicated that I was able to isolate monoclonal antibody that still maintained the specific binding activity. The single-molecule electrical conductance of the protein transistor made by this antibody revealed the dynamic binding which confirmed the thermodynamics of the binding. Production of monoclonal antibodies consists of four steps: immunizing the animal usually a mouse, obtaining immune cells from the spleen of the immunized mouse, fusing the spleen cells with myeloma cells to obtain hybridomas, and selecting the hybridoma cell line producing the desired monoclonal antibody. I immunized mice using gold nanoparticles. The spleens of positive mice were fused with melanoma. The successful fusion cells were properly dilute and monoclonal antibody was produced. To monitor the binding activity, a special ELISA was designed to distinguish the binding activities of this bio-nano interaction. For example, I was able to assay for different interactions such as the IgG-gold surface, IgG-physical size, and IgG-shape. In addition, the bio-nano interaction was detected by the single-molecule electrical conductance platform, for a final confirmation.
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Balakrishnan, Arjun. "Unravelling the Mechanism of Bactericidal/Permeability-Increasing Protein Expression during Bacterial Pathogenesis". Thesis, 2016. http://etd.iisc.ac.in/handle/2005/3155.
Abstract (sommario):
Anti-microbial proteins (AMP) are the key effector arm of the innate immune system. The prevalence of AMP in single-celled eukaryotes to humans shows its importance during the course of evolution. The first report for the role of the anti-microbial peptide in clearing infection was given by Alexander Fleming in 1990’s through the discovery of Penicillin and Lysozyme. The search for antimicrobial agents in human granulocytes was begun by Ehrlich in 1870’s but the first successful isolation of an antimicrobial agent from rabbit neutrophils was done by Zeya and Spitznagel in 1969. Later work by Peter Elshbach and his group on AMPs in rabbit neutrophils brought to light an AMP that can increase the permeability of the bacterial membrane. This AMP named as Bactericidal/permeability-increasing protein (BPI) was further isolated from human neutrophils. Since then many studies have been carried out to understand the mode of action of BPI, which culminated in understanding the new functional activity of this protein viz opsonisation, LPS neutralization and anti-angiogenic function. Knowing to the role of BPI as an anti-inflammatory agent, multiple studies have tried to use BPI for treating endotoxic shock. Dysregulation of BPI expression is associated with various inflammatory diseases like Crohn’s Disease (CD), Ulcerative colitis (UC) and Infectious enteritis’s. Mutations in BPI are also linked to susceptibility to various infections. Even though there are several studies focusing on the functional aspects of BPI, the regulation of BPI expression is poorly understood. Knowing the clinical importance of dysregulation of BPI, it is vital to understand the regulation of BPI expression during the course of bacterial infection.
The Thesis is divided into four chapters. As the main aim of this study is to understand the regulation of BPI expression, in Chapter 1 we introduce the known facts about the protein. A brief overview of the mode of action and regulation of BPI is discussed in this chapter. The subsequent sections describe the diseases associated with Dysregulation of BPI and the use of BPI as a therapeutic agent in various diseases. Towards the end, the objective of the present study is discussed.
BPI is primarily known to be expressed in human neutrophils and epithelial cells. Previous studies have shown that among innate immune cells, murine BPI is expressed only in dendritic cells and neutrophils, but not in macrophages. Based on these results, it was presumed that BPI is not expressed in human macrophages. In Chapter 2, we report the presence of BPI in human macrophages. Our studies revealed increased expression of BPI in human macrophages stimulated with various PAMPs (Pathogen-associated molecular patterns) viz., LPS, flagellin as well as during bacterial infection. Further, during the course of an infection, BPI interacted with Gram-negative bacteria, resulting in enhanced phagocytosis and subsequent control of the bacterial replication. However, it was observed that bacteria which can maintain an active replicating niche (Salmonella Typhimurium) avoid the interaction with BPI during later stages of infection. On the other hand Salmonella mutants, which cannot maintain a replicating niche, as well as Shigella flexneri, which quit the endosomal vesicle, showed interaction with BPI. BPI was induced in both M1 and M2 differentiated macrophages suggesting its role in limiting Gram-negative bacteria and parasitic infection. These results propose an active role of BPI in Gram-negative bacterial clearance by human macrophages. This chapter concludes with a discussion on the importance of BPI expression in human but not murine macrophages. The importance of maintaining an active replicating niche by STM to evade interaction with BPI is also discussed.
As the first line of defense against invading pathogens, intestinal epithelium produces various antimicrobial proteins (AMP) that help with clearance of pathogen. The precise mechanism of AMP regulation in intestinal epithelium is not clear. Intestinal epithelium being a primary entry point for various pathogens, we tried to understand the regulation of BPI expression in the intestine during the course of bacterial infection. In Chapter 3, we report a direct correlation between intestinal damage and BPI expression. In Caco-2 cells, we see a significant increase in BPI levels upon membrane damage mediated by S.aureus infection and pore-forming toxins (Streptolysin and Listeriolysin). Cells detect changes in potassium levels as a Danger-associated molecular pattern (DAMP) associated with cell damage and induce BPI expression in a p38 dependent manner. These results are further supported by in vivo findings that BPI expression in the murine intestinal epithelium is induced upon infection with bacteria which cause intestinal damage (Salmonella Typhimurium & Shigella flexneri) whereas mutants which don’t cause intestinal damage (STM fliC & STM invC), didn’t induce BPI expression. These findings have a huge impact on our current understanding of AMP response during inflammatory bowel diseases (IBD). Our results suggest that dysregulation of BPI expression might be an effect rather than a cause of IBD. This chapter concludes with a discussion on the importance of potassium efflux associated with membrane damage as an important signal that helps in discriminating the invading pathogen from the pool of gut microflora.
Bactericidal/permeability-increasing protein had been shown to possess anti-inflammatory and endotoxin neutralizing activity by interacting with LPS of Gram-negative bacteria. Even though rBPI (recombinant BPI) has cleared phase III clinical trials for treating endotoxemia, the high cost of purified BPI provided by pharmaceutical companies makes it inaccessible or unavailable for the common man. In Chapter 4, we examined the feasibility of using murine BPI (mBPI) expressed on halophilic Archaeal gas vesicle nanoparticles (GVNPs) for the treatment of endotoxemia in high-risk patients, using a murine model of D-galactosamine-induced endotoxic shock. Halobacterium sp. NRC-1 was used to express the N-terminal 199 amino acid residues of mBPI fused to the GVNP GvpC protein, and bound to the surface of the haloarchaeal GVNPs. Our results indicate that delivery of mBPIN-GVNPs increase the survival rate of mice challenged with lethal concentrations of lipopolysaccharide (LPS) and D-galactosamine. Additionally, the mBPIN-GVNP-treated mice displayed reduced symptoms of inflammation including inflammatory anemia, recruitment of neutrophils, liver apoptosis and pro-inflammatory serum cytokine levels. This chapter concludes with a discussion of the advantages of using mBPIN-GVNPs over purified protein in treating endotoxic shock.
6
Balakrishnan, Arjun. "Unravelling the Mechanism of Bactericidal/Permeability-Increasing Protein Expression during Bacterial Pathogenesis". Thesis, 2016. http://hdl.handle.net/2005/3155.
Abstract (sommario):
Anti-microbial proteins (AMP) are the key effector arm of the innate immune system. The prevalence of AMP in single-celled eukaryotes to humans shows its importance during the course of evolution. The first report for the role of the anti-microbial peptide in clearing infection was given by Alexander Fleming in 1990’s through the discovery of Penicillin and Lysozyme. The search for antimicrobial agents in human granulocytes was begun by Ehrlich in 1870’s but the first successful isolation of an antimicrobial agent from rabbit neutrophils was done by Zeya and Spitznagel in 1969. Later work by Peter Elshbach and his group on AMPs in rabbit neutrophils brought to light an AMP that can increase the permeability of the bacterial membrane. This AMP named as Bactericidal/permeability-increasing protein (BPI) was further isolated from human neutrophils. Since then many studies have been carried out to understand the mode of action of BPI, which culminated in understanding the new functional activity of this protein viz opsonisation, LPS neutralization and anti-angiogenic function. Knowing to the role of BPI as an anti-inflammatory agent, multiple studies have tried to use BPI for treating endotoxic shock. Dysregulation of BPI expression is associated with various inflammatory diseases like Crohn’s Disease (CD), Ulcerative colitis (UC) and Infectious enteritis’s. Mutations in BPI are also linked to susceptibility to various infections. Even though there are several studies focusing on the functional aspects of BPI, the regulation of BPI expression is poorly understood. Knowing the clinical importance of dysregulation of BPI, it is vital to understand the regulation of BPI expression during the course of bacterial infection.
The Thesis is divided into four chapters. As the main aim of this study is to understand the regulation of BPI expression, in Chapter 1 we introduce the known facts about the protein. A brief overview of the mode of action and regulation of BPI is discussed in this chapter. The subsequent sections describe the diseases associated with Dysregulation of BPI and the use of BPI as a therapeutic agent in various diseases. Towards the end, the objective of the present study is discussed.
BPI is primarily known to be expressed in human neutrophils and epithelial cells. Previous studies have shown that among innate immune cells, murine BPI is expressed only in dendritic cells and neutrophils, but not in macrophages. Based on these results, it was presumed that BPI is not expressed in human macrophages. In Chapter 2, we report the presence of BPI in human macrophages. Our studies revealed increased expression of BPI in human macrophages stimulated with various PAMPs (Pathogen-associated molecular patterns) viz., LPS, flagellin as well as during bacterial infection. Further, during the course of an infection, BPI interacted with Gram-negative bacteria, resulting in enhanced phagocytosis and subsequent control of the bacterial replication. However, it was observed that bacteria which can maintain an active replicating niche (Salmonella Typhimurium) avoid the interaction with BPI during later stages of infection. On the other hand Salmonella mutants, which cannot maintain a replicating niche, as well as Shigella flexneri, which quit the endosomal vesicle, showed interaction with BPI. BPI was induced in both M1 and M2 differentiated macrophages suggesting its role in limiting Gram-negative bacteria and parasitic infection. These results propose an active role of BPI in Gram-negative bacterial clearance by human macrophages. This chapter concludes with a discussion on the importance of BPI expression in human but not murine macrophages. The importance of maintaining an active replicating niche by STM to evade interaction with BPI is also discussed.
As the first line of defense against invading pathogens, intestinal epithelium produces various antimicrobial proteins (AMP) that help with clearance of pathogen. The precise mechanism of AMP regulation in intestinal epithelium is not clear. Intestinal epithelium being a primary entry point for various pathogens, we tried to understand the regulation of BPI expression in the intestine during the course of bacterial infection. In Chapter 3, we report a direct correlation between intestinal damage and BPI expression. In Caco-2 cells, we see a significant increase in BPI levels upon membrane damage mediated by S.aureus infection and pore-forming toxins (Streptolysin and Listeriolysin). Cells detect changes in potassium levels as a Danger-associated molecular pattern (DAMP) associated with cell damage and induce BPI expression in a p38 dependent manner. These results are further supported by in vivo findings that BPI expression in the murine intestinal epithelium is induced upon infection with bacteria which cause intestinal damage (Salmonella Typhimurium & Shigella flexneri) whereas mutants which don’t cause intestinal damage (STM fliC & STM invC), didn’t induce BPI expression. These findings have a huge impact on our current understanding of AMP response during inflammatory bowel diseases (IBD). Our results suggest that dysregulation of BPI expression might be an effect rather than a cause of IBD. This chapter concludes with a discussion on the importance of potassium efflux associated with membrane damage as an important signal that helps in discriminating the invading pathogen from the pool of gut microflora.
Bactericidal/permeability-increasing protein had been shown to possess anti-inflammatory and endotoxin neutralizing activity by interacting with LPS of Gram-negative bacteria. Even though rBPI (recombinant BPI) has cleared phase III clinical trials for treating endotoxemia, the high cost of purified BPI provided by pharmaceutical companies makes it inaccessible or unavailable for the common man. In Chapter 4, we examined the feasibility of using murine BPI (mBPI) expressed on halophilic Archaeal gas vesicle nanoparticles (GVNPs) for the treatment of endotoxemia in high-risk patients, using a murine model of D-galactosamine-induced endotoxic shock. Halobacterium sp. NRC-1 was used to express the N-terminal 199 amino acid residues of mBPI fused to the GVNP GvpC protein, and bound to the surface of the haloarchaeal GVNPs. Our results indicate that delivery of mBPIN-GVNPs increase the survival rate of mice challenged with lethal concentrations of lipopolysaccharide (LPS) and D-galactosamine. Additionally, the mBPIN-GVNP-treated mice displayed reduced symptoms of inflammation including inflammatory anemia, recruitment of neutrophils, liver apoptosis and pro-inflammatory serum cytokine levels. This chapter concludes with a discussion of the advantages of using mBPIN-GVNPs over purified protein in treating endotoxic shock.
Capitoli di libri sul tema "Nano-Antibody":
1
Chen, Yeong-Renn. "EPR Spin-Trapping and Nano LC MS/MS Techniques for DEPMPO/•OOH and Immunospin-Trapping with Anti-DMPO Antibody in Mitochondrial Electron Transfer System". In Methods In Molecular Biology, 75–88. Totowa, NJ: Humana Press, 2008. http://dx.doi.org/10.1007/978-1-60327-517-0_7.
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Ignatov, Sergei Georgievich, S. Yu Filippovich e Ivan Alekseevich Dyatlov. "Specific Immobilization of Rotaviruses for Atomic Force Microscopy Using Langmuir Antibody Films Based on Amphiphilic Polyelectrolytes". In Macro, Micro, and Nano-Biosensors, 117–31. Cham: Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-030-55490-3_7.
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Mokhtari, Wafaa, Mohamed Achouri, Abdellah Remah, Noureddine Chtaina e Hassan Boubaker. "Nano-Biosensors Tech and IPM in Plant Protection to Respond to Climate Change Challenges in Morocco". In Sensor Network Methodologies for Smart Applications, 114–29. IGI Global, 2020. http://dx.doi.org/10.4018/978-1-7998-4381-8.ch005.
Abstract (sommario):
In this chapter, the authors introduce two research axes: Part A, nano-biosensors as ad-hoc technologies designed to meet plant diagnostic sensitivity and specificity needs at point of care, and Part B, the study of the interaction of drought and infection stresses in crops investigating bio-control potential antagonists in developing integrated approach (IPM) for disease control measures in crops system. The first part will be revising most used nano-biosensors in plant pathogens detection using different platforms in greenhouses, on-field, and during postharvest. A special focus will be on optical and voltametric immuno/DNA sensors application in plant protection. The last part will present case studies of using nanoparticles functionalized with antibody/DNA for detecting pathogenic Pseudomonas sp, mosaic viruses, Botrytis cinereal, and Fusarium mycotoxins (DON). The second part will be interpreting experimental results of a case study on evaluating bio-control efficacy of local Trichoderma spp. using root dips treatment in Fusarium solani-green beans pathosystem as a model.
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Bhattacharya, Sankha, e Kapil Gore. "Targeted Cancer Therapy Using Nanoparticles and Antibody Fragments". In Advances in Precision Medicine Oncology. IntechOpen, 2021. http://dx.doi.org/10.5772/intechopen.96550.
Abstract (sommario):
Cancer is caused by an uncontrolled cell division, forming a tumor capable of metastasis. Cancer is the second leading cause of death worldwide. Conventional treatments kill healthy cells, causing side effects. Recently, nanomaterials are explored due to properties such as as- nano-size, high loading, and ligands’ attachment for a selective delivery. Apart from normal body cells, cancer cells express many receptors in excess, which serve as ‘targets’ for attacking the cells. Various ligands like proteins, peptides, polysaccharides can be attached to nanoparticles to allow proper and specific reach to the tumor. Such nanoparticles go to their desired site and stick onto the receptors, taken inside the cells by various methods. Antibodies are natural proteins that bind to foreign substances and remove them. IgG being the most explored antibody, suffers from many disadvantages such as non-specificity for required antigen, limited binding sites, low tumor penetration. Hence many researchers experimented by removing and adjusting the binding sites, using only the binding sites, enhancing the valency of naturally available IgG. It gave many benefits such as enhanced penetration, reduced immunogenicity, better delivery of drugs with fewer side effects. Continuing advancements in the field of protein engineering will help scientists to come up with better solutions. The properties allow easy surface interaction and entry, achieve better biodistribution, and reduce the amount of drug required. Targeting is based on Paul Ehrlich’s ‘magic bullet, ‘where the therapeutic moiety has two parts-one to identify the target and the second to eliminate it. This concept is revised to incorporate a third component, a carrier. Many nanocarriers can be used to target cancer cells containing ligands to identify malignant cells. Approaches to targeting are passive, active and physical targeting. Many such nanoparticles are in clinical trials and can be a better solution to cancer therapy.
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Mukherjee, D. "ZnO for Probes in Diagnostics". In ZnO and Their Hybrid Nano-Structures, 202–33. Materials Research Forum LLC, 2023. http://dx.doi.org/10.21741/9781644902394-7.
Abstract (sommario):
Nanoparticles have revolutionized the field of diagnostics in recent years and ZnO nanoparticles (ZnO-NPs) have been one of the most commonly used ones. These easily synthesizable ZnO-NPs have a multitude of advantages over other metal-based nanoparticles owing to their biocompatibility, easy functionalization through their hydroxyl group-rich surface, and cost-effectiveness among several other benefits. Due to their inherent luminescence and fluorescent-tag functionalizing properties, ZnO-NPs have been useful as a probe in tumour and live cell bioimaging. ZnO-NPs have also been identified as probes in biosensors for the detection of various clinically important biochemical analytes like glucose and cholesterol, pathogens, drug molecules, and antibody-antigen based detection systems. In this chapter, several of the different applications of ZnO as probes in diagnostics will be dealt with in detail. Also, the characteristics of ZnO nanoparticles useful for such applications and the way these devices and techniques are developed will be explained.
Atti di convegni sul tema "Nano-Antibody":
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Ding, Ann-Ann, Ying-Yi Chen, Churng-Ren Chris Wang, Pai-Chi Li e Dar-Bin Shieh. "HER-2 Antibody Conjugated Gold Nano Rod for in Vivo Photothermal Therapy". In 2008 8th IEEE Conference on Nanotechnology (NANO). IEEE, 2008. http://dx.doi.org/10.1109/nano.2008.264.
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Ishijima, Ayumu, Shinya Yamahira, Satoshi Yamaguchi, Etsuko Kobayashi, Yoshikazu Shibasaki, Takashi Azuma, Teruyuki Nagamune e Ichiro Sakuma. "Notice of Removal: Antibody-conjugated phase-change nano-droplet for ultrasound therapeutic agent". In 2017 IEEE International Ultrasonics Symposium (IUS). IEEE, 2017. http://dx.doi.org/10.1109/ultsym.2017.8092636.
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Uda, M. N. A., C. M. Hasfalina, A. A. Samsuzanaa, S. Faridah, I. Zamri, B. Siti Noraini, W. Nur Sabrina, U. Hashim e Subash C. B. Gopinath. "Immunosensor development formatting for tungro disease detection using nano-gold antibody particles application". In 11TH ASIAN CONFERENCE ON CHEMICAL SENSORS: (ACCS2015). Author(s), 2017. http://dx.doi.org/10.1063/1.4975290.
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Sha, Jingjie, Fangzhou Fu, Bing Xu, Ke Chen e Xiao Li. "Evaluation of the Binding of PD-1 Antibody and Antigen Using Nano-Sensors". In 2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems & Eurosensors XXXIII (TRANSDUCERS & EUROSENSORS XXXIII). IEEE, 2019. http://dx.doi.org/10.1109/transducers.2019.8808675.
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Choi, Eunpyo, Yuri Choi e Jungyul Park. "Specific and label-free immunoglobulin G antibody detection using nano porous hydrogel photonic crystals". In TRANSDUCERS 2011 - 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference. IEEE, 2011. http://dx.doi.org/10.1109/transducers.2011.5969328.
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Uda, M. N. A., C. M. Hasfalina, A. A. Samsuzana, U. Hashim, Shahrul A. B. Ariffin, Zamri I., Nur Sabrina W. et al. "Immunosensor development for rice tungro bacilliform virus (RTBV) detection using antibody nano-gold conjugate". In 11TH ASIAN CONFERENCE ON CHEMICAL SENSORS: (ACCS2015). Author(s), 2017. http://dx.doi.org/10.1063/1.4975291.
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OHUCHI, NORIAKI, MORIO NAKAJIMA, HIROSHI TADA, TAKANORI ISHIDA, MOTOHIRO TAKEDA e HIDEO HIGUCHI. "NANO-SENSING CAPSULES FOR MEDICAL APPLICATION: NANO-PARTICLES FOR SENTINEL NAVIGATION AND QUANTUM DOTS CONJUGATION WITH ANTI-HER2 ANTIBODY FOR MOLECULAR IMAGING OF CANCER". In Proceedings of the Final Symposium of the Tohoku University 21st Century Center of Excellence Program. IMPERIAL COLLEGE PRESS, 2006. http://dx.doi.org/10.1142/9781860948800_0027.
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Tseng, Shin-Hua, Dion T. Tseng, Tzu-Cheng Lee, Tsai-Mu Cheng, Jyh-Yuan Yang, Ruo-Yu Hsieh, Chuan-Mei Tsai e Chia-Ching Chang. "Ultra Sensitive Detection of Eneterovirus 71 by Modified Electrochemical Impedance Spectroscopy". In ASME 2010 First Global Congress on NanoEngineering for Medicine and Biology. ASMEDC, 2010. http://dx.doi.org/10.1115/nemb2010-13143.
Abstract (sommario):
The enterovirus 71 (EV71) has threatened Taiwan for more than ten years. Since traditional diagnostic methods are complicated, time consuming, and high-qualified personnel required. Therefore, a new detection process is highly desired. In this study, a high sensitive PC-based electrochemical analyzing system with a functionalized nano-gold modified immunological electrode are developed to detect EV71. Immobilizing specific EV71 polyclone antibodies, which is developed by center for disease control (CDC) Taiwan, onto nano gold of sensing electrode, the affinity interaction of the immobilized antibody with the specific antigen is identified quickly by electrochemical impedance spectrum (EIS) within 20 minutes. The detection limit of this EIS analysis was as low as 50 copy per ml (∼sub-atto molar). In summary, a biosensor and analyzing system based on EIS has been developed to identify EV71 with efficiency, high sensitivity and specificity.
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Mallakin, Ali, Kazushi Inoue e Martin Guthold. "In-Situ Quantitative Analysis of Tumor Suppressor Protein (hDMP1) Using a Nanomechanical Cantilever Beam". In ASME 2005 International Design Engineering Technical Conferences and Computers and Information in Engineering Conference. ASMEDC, 2005. http://dx.doi.org/10.1115/detc2005-84503.
Abstract (sommario):
This study is focused on testing “immuno-sensors” of micro-cantilever beams for the purpose of future design of high-throughout bioassays. We currently study the aberrant expression, deletion and mutation of hDMP1 (Human DMP1) in human lung cancer. Lung cancer is the leading cause of cancer deaths among men and women in North America. There are four major histological subtypes of human lung cancer: small-cell carcinoma (SCC), adenocarcinoma (AC), squamous cell carcinoma (SCC), and large-cell carcinoma (LCC). The hDMP1 locus is localized on chromosome 7q21, a region frequently deleted as part of the 7q-minus and monosomy 7 abnormalities of acute myeloid leukemia and myelodysplastic syndrome. Recent data demonstrate the critical role of Dmp1 in Ras-Raf-Arf signaling and cellular senescence. In order to study the interactions of hDMP1 gene product and selected tumor markers with BioMEMS devices, protein coating (Antibody) conduct on cantilevers with silicon nitride (SiNx) surfaces. Silicon nitride surface has the potential to provide a good interface for BioMEMS devices, due to enhanced adherence of substances on this surface. The cantilever biosensors coated with hDMP1 antibody were used for the detection of hDMP1 antigen, which is known to be a tumor suppressor protein. Results revealed that the changes in nano-mechanical forces provided sufficient differential torque to bend the cantilever beam upon injection of the antigen. Theoretical models have been developed for the prediction of the vibrational responses of atomic force microscope (AFM) cantilevers before and after antigen/antibody interaction. Exposure of the proteins to micro-cantilever (MC) resulted in un-reversible large stress. Static deflection of micro-cantilever appeared as a result of the surface stresses that are induced by changes upon antibody-antigen interaction. This indicated that the micro-cantilever approach is useful for detecting proteins and tumor markers, and this system is applicable as a model to design miniaturized biosensor systems. We also applied gene micro-array to identify unknown targets for hDMP1 and extend our observation of the complicated process of carcinogenesis.
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Wang, Wei-Jhen, Chia-Hwa Lee, Chin-Wen Li, Stephen Liao, Fuh-Jyh Jan e Gou-Jen Wang. "Direct Label Free Detection of Orchid Virus Using a Micro/Nano Hybrid Structured Biosensor". In ASME 2019 International Design Engineering Technical Conferences and Computers and Information in Engineering Conference. American Society of Mechanical Engineers, 2019. http://dx.doi.org/10.1115/detc2019-97198.
Abstract (sommario):
Abstract In this study, a label-free detection approach for effective detection of the odontoglossum ringspot virus (ORSV) infected orchids has been developed. We used semiconductor fabrication process to fabricate 1,810 micro/nano hybrid structured sensing electrodes on a 8 inch reclaimed wafer. The self-assembled monolayer (SAM) process was then employed to sequentially modify the electrode surface with 11-mercaptoundecanoic acid (11-MUA), 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC)/ N-hydroxysuccinimide (NHS), anti-ORSV, and ORSV. EIS was conducted for the ORSV concentration detection. Experimental results demonstrated that the ORSV concentration in a virus infected orchid leaf could be effectively detected. When compared with the ELISA kit, our device possesses a wider linear detection range (0.5–50,000 ng/mL) and a higher sensitivity. The specificity of our device on ORSV detection was also confirmed. Our sensing device retains advantages, such as label-free, lower amounts of the antibody and target sample required, low detection time, and a wider linear detection range. Those results imply the feasibility of our sensing device in field applications.