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Letteratura scientifica selezionata sul tema "Mycobacterium bovis"

Autore: Grafiati

Pubblicato: 4 giugno 2021

Ultima modifica: 29 luglio 2024

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Articoli di riviste sul tema "Mycobacterium bovis"

1

KRYSZTOPA-GRZYBOWSKA, KATARZYNA, SYLWIA BRZEZIŃSKA, EWA AUGUSTYNOWICZ-KOPEĆ, EWA AUGUSTYNOWICZ e ANNA LUTYŃSKA. "PCR-Based Genomic Deletion Analysis of RD-Regions in the Identification of Mycobacteria Isolated from Adverse Events Following BCG Vaccination or TB Suspected Cases". Polish Journal of Microbiology 63, n. 3 (2014): 359–62. http://dx.doi.org/10.33073/pjm-2014-048.

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Early identification of mycobacterial species is crucial for early diagnosis. PCR-multiplex method performed on randomly chosen 54 mycobacteria isolates originating from clinical samples was found to be an inexpensive, quick and reliable alternative for commercially available diagnostics tests. Although the results of gene probes identification performed by NTLDR were generally consistent with multiplex PCR, two mixed Mycobacterium bovis BCG/Mycobacterium tuberculosis infections and a single misdiagnosis of M. tuberculosis with M. bovis were found. The routine application of multiplex-PCR has the potential to make diagnostics surveillance studies feasible.

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2

Waters, W. R., B. J. Nonnecke, M. V. Palmer, S. Robbe-Austermann, J. P. Bannantine, J. R. Stabel, D. L. Whipple et al. "Use of Recombinant ESAT-6:CFP-10 Fusion Protein for Differentiation of Infections of Cattle by Mycobacterium bovis and by M. avium subsp. avium and M. avium subsp. paratuberculosis". Clinical Diagnostic Laboratory Immunology 11, n. 4 (luglio 2004): 729–35. http://dx.doi.org/10.1128/cdli.11.4.729-735.2004.

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ABSTRACT Immunological diagnosis of Mycobacterium bovis infection of cattle is often confounded by cross-reactive responses resulting from exposure to other mycobacterial species, especially Mycobacterium avium. Early secretory antigenic target 6 (ESAT-6) and culture filtrate protein 10 (CFP-10) are dominant gamma interferon (IFN-γ)-inducing antigens of tuberculous mycobacteria, and they are absent from many environmental nontuberculous mycobacteria. Because M. avium exposure is the primary confounding factor in the diagnosis of M. bovis-infected animals, in vitro responses to a recombinant ESAT-6:CFP-10 (rESAT-6:CFP-10) fusion protein by blood leukocytes from cattle naturally exposed to M. avium or experimentally challenged with Mycobacterium avium subsp. avium or Mycobacterium avium subsp. paratuberculosis were compared to responses by M. bovis-infected cattle. Responses to heterogeneous mycobacterial antigens (i.e., purified protein derivatives [PPDs] and whole-cell sonicates [WCSs]) were also evaluated. Tumor necrosis factor alpha (TNF-α), IFN-γ, and nitric oxide responses by M. bovis-infected cattle to rESAT-6:CFP-10 exceeded (P < 0.05) the corresponding responses by cattle naturally sensitized to M. avium. Experimental infection with M. bovis, M. avium, or M. avium subsp. paratuberculosis induced significant (P < 0.05) IFN-γ and nitric oxide production to WCS and PPD antigens, regardless of the mycobacterial species used for the preparation of the antigen. Responses to homologous crude antigens generally exceeded responses to heterologous antigens. Nitric oxide and IFN-γ responses to rESAT-6:CFP-10 by blood leukocytes from M. bovis-infected calves exceeded (P < 0.05) the corresponding responses of noninfected, M. avium-infected, and M. avium subsp. paratuberculosis-infected calves. Despite the reported potential for secretion of immunogenic ESAT-6 and CFP-10 proteins by M. avium and M. avium subsp. paratuberculosis, it appears that use of the rESAT-6:CFP-10 fusion protein will be useful for the detection of tuberculous cattle in herds with pre-existing sensitization to M. avium and/or M. avium subsp. paratuberculosis.

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3

Waters, W. R., A. O. Whelan, K. P. Lyashchenko, R. Greenwald, M. V. Palmer, B. N. Harris, R. G. Hewinson e H. M. Vordermeier. "Immune Responses in Cattle Inoculated with Mycobacterium bovis, Mycobacterium tuberculosis, or Mycobacterium kansasii". Clinical and Vaccine Immunology 17, n. 2 (9 dicembre 2009): 247–52. http://dx.doi.org/10.1128/cvi.00442-09.

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ABSTRACT Cattle were inoculated with Mycobacterium bovis, Mycobacterium tuberculosis, or Mycobacterium kansasii to compare the antigen-specific immune responses to various patterns of mycobacterial disease. Disease expression ranged from colonization with associated pathology (M. bovis infection) and colonization without pathology (M. tuberculosis infection) to no colonization or pathology (M. kansasii infection). Delayed-type hypersensitivity and gamma interferon responses were elicited by each mycobacterial inoculation; however, the responses by the M. bovis- and M. tuberculosis-inoculated animals exceeded those of the M. kansasii-inoculated animals. Specific antibody responses were detected in all M. tuberculosis- and M. bovis-inoculated cattle 3 weeks after inoculation. From 6 to 16 weeks after M. tuberculosis inoculation, the antibody responses waned, whereas the responses persisted with M. bovis infection. With M. kansasii inoculation, initial early antibody responses waned by 10 weeks after inoculation and then increased 2 weeks after the injection of purified protein derivative for the skin test at 18 weeks after challenge. These findings indicate that antibody responses are associated with the antigen burden rather than the pathology, cellular immune responses to tuberculin correlate with infection but not necessarily with the pathology or bacterial burden, and exposure to mycobacterial antigens may elicit an antibody response in a presensitized animal.

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4

Aldwell, Frank E., Bridget L. Dicker, Fernanda M. Da Silva Tatley, Martin F. Cross, Simon Liggett, Colin G. Mackintosh e J. Frank T. Griffin. "Mycobacterium bovis-Infected Cervine Alveolar Macrophages Secrete Lymphoreactive Lipid Antigens". Infection and Immunity 68, n. 12 (1 dicembre 2000): 7003–9. http://dx.doi.org/10.1128/iai.68.12.7003-7009.2000.

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ABSTRACT Tuberculosis is caused by intracellular bacteria belonging to the genus Mycobacterium, including M. tuberculosisand M. bovis. Alveolar macrophages (AMs) are the primary host cell for inhaled mycobacteria. However, little is known about the mechanisms by which infected AMs can process and present mycobacterial antigens to primed lymphocytes and how these responses may affect ensuing protection in the host. In the present study, we sought to determine whether AMs from a naturally susceptible host forMycobacterium bovis (red deer) could produce and secrete soluble immunoreactive antigens following mycobacterial infection in vitro. Confluent monolayers of deer AMs were infected with either heat-killed or live virulent M. bovis or M. bovis BCG at a multiplicity of infection of 5:1 and cultured for 48 h. Culture supernatants were collected, concentrated, and tested for the presence of mycobacterial antigens in a lymphocyte proliferation assay by using peripheral blood mononuclear cells fromM. bovis-sensitized or naive deer. Supernatants derived from macrophages which had been infected with live bacilli stimulated the proliferation of antigen-sensitized, but not naive, lymphocytes. Supernatants derived from uninoculated AMs or AMs inoculated with heat-killed bacilli failed to stimulate lymphocyte proliferation. The lymphoproliferative activity was retained following lipid extraction of the supernatants, which were free of amino groups as determined by thin-layer chromatography. These results demonstrate that mycobacteria which are actively growing within AMs produce lipids which are secreted into the extracellular milieu and that these lipids are recognized by lymphocytes from mycobacterium-primed hosts. We suggest that mycobacterial lipids are released from AMs following aerosol infection in vivo and that they play an important role in the early immune response to tuberculosis.

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5

Ekladious, Adel. "Disseminated Mycobacterium Bovis". International Journal of Clinical Case Reports and Reviews 11, n. 4 (21 luglio 2022): 01–03. http://dx.doi.org/10.31579/2690-4861/232.

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Intravesical BacillusChalmette- Guerin (BCG) still a popular medication for non-invasive bladdercancer in the low-income country, one of the uncommon side effects is disseminated Mycobacterium Bovis. We present a patient who presented with haematuria, diagnosed as urothelial superficial bladder cancer, treated with incomplete resectionand intravesical BCG, 6 months after treatment, he presented with increasing shortness of breath, headache and abdominal pain, diagnosed as tuberculous, meningitis, massive pleural effusion, granulomatous hepatitis he responded very well to anti tuberculous treatment.

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6

Stern, Rebecca, Clay Roscoe e Elizabeth A. Misch. "<i>Mycobacterium bovis</i> BCG osteoarticular infection complicating immune therapy for bladder cancer: a case report". Journal of Bone and Joint Infection 6, n. 4 (22 febbraio 2021): 107–10. http://dx.doi.org/10.5194/jbji-6-107-2021.

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Abstract. Osteoarticular infection with Mycobacterium bovis (M. bovis) is a rare complication of bladder cancer treatment with intravesical Bacillus Calmette–Guèrin (BCG). We describe a case of disseminated Mycobacterium bovis BCG infection masquerading as a chronic prosthetic joint infection in a patient with several risk factors for progressive mycobacterial infection.

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7

Erb, Klaus J., Claudia Trujillo, Mike Fugate e Heidrun Moll. "Infection with the Helminth Nippostrongylus brasiliensis Does Not Interfere with Efficient Elimination of Mycobacterium bovis BCG from the Lungs of Mice". Clinical and Vaccine Immunology 9, n. 3 (maggio 2002): 727–30. http://dx.doi.org/10.1128/cdli.9.3.727-730.2002.

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ABSTRACT Infection with Mycobacterium tuberculosis continues to be one of the major global health threats. Strong mycobacterium-specific Th1 immune responses correlate with protection, and decreased Th1 responses correlate with disease progression. In contrast, the impact of Th2 responses on the development of protective immune responses to mycobacteria remains unclear. To analyze whether ongoing Th2 responses present in the lung influence the development of a protective Th1 immune response to mycobacteria, we coinfected mice with the helminth Nippostrongylus brasiliensis and Mycobacterium bovis BCG. We found that the T cells from the lymph nodes of coinfected mice secreted significantly less gamma interferon than did the T cells from mice infected with M. bovis BCG after in vitro stimulation with purified protein from M. tuberculosis when 108 CFU of M. bovis BCG were used for the infection. This result indicates that the helminth infection reduced the Th1 immune response to the mycobacteria in the lung. However, mycobacterial clearance was not delayed in the coinfected animals. Importantly, the infection with BCG after the helminth infection did not reduce the helminth-induced Th2 response in the lung, ruling out the possibility that the lack of a reduction in bacterial clearance in the coinfected mice was due to a downmodulation of the helminth-induced Th2 response. Taken together, our results suggest that ongoing Th2 responses in the lung do not necessarily lead to increased susceptibility to mycobacterial infection.

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8

Da Silva Pedroso, Silvia Cristina, Karla Valéria Batista Lima, Ismari Perini Furlaneto, Yan Corrêa Rodrigues, Darlene Kássia Saraiva Queiroz Pantoja, Alex Junior Souza de Souza e Washington Luiz Assunção Pereira. "Bovine tuberculosis due to Mycobacterium bovis and other mycobacteria among water buffalo (Bubalus bubalis) from the Brazilian Amazon". Journal of Infection in Developing Countries 15, n. 05 (31 maggio 2021): 736–41. http://dx.doi.org/10.3855/jidc.13558.

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Introduction: Zoonotic tuberculosis is a disease of public health importance worldwide, especially in developing countries. The present study aimed to investigate the role played by Mycobacterium bovis and other mycobacteria as etiologic agents of bubaline tuberculosis (TB) in the Brazilian Amazon region. Methodology: Granulomatous lesions suggestive of TB obtained from 109 buffaloes (n =109) during sanitary inspection at slaughter were subjected to histopathological evaluation, immunohistochemical (IHC) detection of Mycobacterium antigens, and to molecular tests (PCR) to detect hsp65, IS6110 and RD4 genes, which are specific to Mycobacterium spp., Mycobacterium tuberculosis Complex (MTBC) and M. bovis, respectively. Results: PCR results indicated Mycobacterium infection in 87.2% of the cases, of which 69.5% were positive for M. bovis, 27.4% belonged to MTBC, and 3.1% were probably non-TB mycobacteria. There was good agreement between the genus-specific molecular technique and the histopathological analysis. This high frequency of TB cases caused by non-M. bovis suggests a diversified scenario of mycobacteria associated with bubaline TB in the Brazilian Amazon region. Conclusions: The results reinforce the need of discussing the inclusion of more accurate techniques in examinations carried out by Inspection Services in Brazil.

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Gobin, Jovana, Diane K. Wong, Bradford W. Gibson e Marcus A. Horwitz. "Characterization of Exochelins of theMycobacterium bovis Type Strain and BCG Substrains". Infection and Immunity 67, n. 4 (1 aprile 1999): 2035–39. http://dx.doi.org/10.1128/iai.67.4.2035-2039.1999.

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ABSTRACT Pathogenic mycobacteria must acquire iron in the host in order to multiply and cause disease. To do so, they release abundant quantities of siderophores called exochelins, which have the capacity to scavenge iron from host iron-binding proteins and deliver it to the mycobacteria. In this study, we have characterized the exochelins of Mycobacterium bovis, the causative agent of bovine and occasionally of human tuberculosis, and the highly attenuated descendant of M. bovis, bacillus Calmette-Guérin (BCG), widely used as a vaccine against human tuberculosis. The M. bovis type strain, five substrains ofM. bovis BCG (Copenhagen, Glaxo, Japanese, Pasteur, and Tice), and two strains of virulent Mycobacterium tuberculosis all produce the same set of exochelins, although the relative amounts of individual exochelins may differ. Among these mycobacteria, the total amount of exochelins produced is greatest in M. tuberculosis, intermediate in M. bovis, and smallest in M. bovis BCG.

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DIDKOWSKA, ANNA, PIOTR ŻMUDA, BLANKA ORŁOWSKA e KRZYSZTOF ANUSZ. "Mycobacterial infections in cats (Felis catus) as a potential threat to humans – a review 2014–2023". Medycyna Weterynaryjna 79, n. 12 (2023): 6842–2023. http://dx.doi.org/10.21521/mw.6842.

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Mycobacteria infections in cats include tuberculosis (caused by Mycobacterium bovis and Mycobacterium microti) and mycobacteriosis caused by non-tuberculous mycobacteria (NTM). The aim of the paper is to present the latest reports on mycobacterial infections in cats and place emphasis on their impact on the health of their owners. The reviewers looked for papers about mycobacterial infections in cats in PubMed and Google Scholar from any date from January 2014 to June 2023. The search used the following keywords: cat, feline, tuberculosis, and mycobacteria. Papers were evaluated for their value to science and their applicability. Papers published in recent years have shown that mycobacterial infections in cats should still be considered in a differential diagnosis when many clinical signs present and they are mainly skin and ocular symptoms. An epidemiological investigation of these infections is highly important because cases were reported also in low-risk regions. Mycobacterial infections pose a risk to humans. The degree of risk depends on many factors, such as the species of mycobacteria, the closeness of animal-owner contact, and the immune status of the owner. The greatest risk are still believed to be M. bovis infections; however, NTM infections should also raise a concern, especially in high-risk groups.

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Più fonti

Tesi sul tema "Mycobacterium bovis"

1

Roring, Solvig Mary Margaret. "DNA fingerprinting of Mycobacterium bovis". Thesis, Queen's University Belfast, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.287426.

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2

Mardare, Cornelia. "Interactions of Mycobacterium bovis with protozoa and the occurrence of Mycobacterium bovis in environmental protozoa". Thesis, University of Surrey, 2010. http://epubs.surrey.ac.uk/844633/.

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Bovine tuberculosis is in the UK a persistent disease, affecting cattle and badgers. The latter is suspected to be a reservoir for Mycobacterium bovis but the transmission between badgers and cattle remains unclear. Mycobacteria have been shown to survive ingestion by protozoa and some even multiplied inside amoebas. The aim of this study was to investigate some interactions of Acanthamoeba castellanii and Tetrahymena pyriformis with M bovis. Firstly, the long term survival of the bacilli in protozoa was monitored. Secondly, it was investigated whether bacilli internalized in protozoa cysts are protected from hypochlorous acid and desiccation. Thirdly, the identification of M bovis in environmental protozoa isolated from badger latrines was attempted. The long term incubation of M. bovis with A. castellanii showed that the amoebas had a negative effect on the survival of virulent M. bovis. M. bovis was not detectable after 6 months of coincubation but remained viable at high concentrations in the control experiments. This effect however, could not be seen in T. pyriformis. Cysts of A. castellanii did not protect M bovis from hypochlorous acid and desiccation. Results indicate that M. bovis was more susceptible to hypochlorous acid after the encystment in comparison with the controls. These findings suggest that A. castellanii contributes to the decrease of M. bovis and therefore, it can be suggested that protozoa might have a negative impact on the survival M. bovis in the environment. In one of the samples taken from Woodchester Park, acid fast rods could be identified. Acid fast microorganisms were also identified in trophozoites of protozoa. This indicates that trophozoites of enviromnental protozoa might be carrier of mycobacteria and possibly M. bovis. An infection with bacilli-containing trophozoites might therefore be a potential route of transmission between the enviromnent and animals.

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3

Sales, Mariana Lázaro. "Identificação de Mycobacterium bovis e Mycobacterium tuberculosis por PCR". Universidade Federal de Minas Gerais, 2012. http://hdl.handle.net/1843/SMOC-9L2PT7.

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Abstract (sommario):

Mycobacterium bovis and Mycobacterium tuberculosis are the causative agents of bovine and human tuberculosis, respectively. The standard diagnosis is based on the isolation and identification of bacterial colonies through biochemical methods. These methodologies in addition to being costly, require an environment with high level of biosecurity and a great time of cultivation and tests to obtain the results. The molecular tests based on the principles of real-time PCR using the fluorophore Eva Green were efficient in the identification of M. bovis and M. tuberculosis. In addition, analyses were performed with primers and molecular markers, described in the literature, able to differentiate the M. bovis of M. tuberculosis by PCR. The results showed that some of the molecular markers are found in both microorganisms. The work of this dissertation has enabled the development of real-time PCR techniques able to identify colonies of M. bovis and M. tuberculosis.
O Mycobacterium bovis e o Mycobacterium tuberculosis são os agentes causadores da tuberculose bovina e humana, respectivamente. O diagnóstico padrão se baseia no isolamento bacteriano e a identificação das colônias através de métodos bioquímicos. Estas metodologias além de serem onerosas, requerem um ambiente com alto nível de biossegurança e um grande tempo de cultivo e testes para obtenção dos resultados. Os testes moleculares baseados nos princípios da PCR em tempo real utilizando-se o fluoróforo Eva Green foram eficientes na identificação do M. bovis e M. tuberculosis. Além disso, foram realizadas análises com iniciadores e marcadores moleculares, descritos na literatura, capazes de diferenciar o M. bovis do M. tuberculosis por PCR. Os resultados mostraram que alguns dos marcadores moleculares são encontrados em ambos os micro-organismos. O trabalho dessa dissertação permitiu o desenvolvimento de técnicas de PCR em tempo real capazes de identificar colônias de M. bovis e M. tuberculosis, em substituição aos testes bioquímicos.

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4

Hamerman, Jessica Ann. "Macrophage activation during Mycobacterium bovis BCG infection /". Thesis, Connect to this title online; UW restricted, 2001. http://hdl.handle.net/1773/8359.

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5

Michell, Stephen Lloyd. "Molecular characterisation of a novel lipoglycoprotein from Mycobacterium tuberculosis and Mycobacterium bovis". Thesis, Imperial College London, 1999. http://hdl.handle.net/10044/1/7477.

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Abstract (sommario):

In Great Britain a recent independent scientific review into bovine tuberculosis concluded that the best prospect for control of TB in the national herd is to develop a vaccine. The secreted antigens of the Mycobacterium tuberculosis complex have received much interest as possible vaccine candidates due to their ability to confer protection against tuberculosis in small animal models. The recent discovery that some of these mycobacterial antigens are glycosylated, a modification ubiquitous in eukaryotic proteins, may provide some novel insights into the properties of these antigens. The antigen MPB70 is the major secreted protein of Mycobacterium bovis, the causative agent of tuberculosis in cattle. It has previously been reported that this antigen, encoded for by the gene mpb70, is present in at least two forms, a 22 kDa unglycosylated form and a 25 kDa glycosylated form. A clone was isolated from an M. tuberculosis H37Rv mycobacterial shuttle cosmid library which expressed both the 22 and 25 kDa antigens. Genetic analysis of this cosmid revealed that the two antigens were encoded by separate genes. The gene encoding the 25 kDa antigen (MPT83), subsequently designated mpt83, is situated 2.4 kb upstream of mpt70 and transcribed in the same direction. Using a mycobacterial expression system and alkaline phosphate (PhoA) fusions, it was shown that MPT83 but not MPT70 is glycosylated by Mycobacterium smegmatis. Moreover, neither fusion protein was glycosylated in E. cW demonstrating that glycosylation of MPT83 was specific to the mycobacterial species. The use of site directed mutagenesis in conjunction with the PhoA reporter system identified both 0 and N-linked glycosylation sites within MPT83. Preliminary investigations into the role of glycosylation in the alteration of immune recognition showed a possible influence of cilycosylation on a T cell epitope of MPT83.

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Rennie, Bryan D. "Detection and identification of antigens from Mycobacterium bovis culture filtrate with immune sera from Mycobacterium bovis sensitized or infected cattle". Thesis, University of Ottawa (Canada), 2009. http://hdl.handle.net/10393/28138.

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Bovine tuberculosis, caused by Mycobacterium bovis, infects approximately 50 million cattle worldwide and is diagnosed by the tuberculin skin test (TST). The purpose of this thesis was to characterise the culture filtrate proteins (CFP) of M. bovis PPD tuberculin and to compare the antibody response of M. bovis infected versus M. bovis sensitized cattle. Sterile filtered PPD tuberculin (SF-PPD) resolved into approximately 200 discrete spots using two-dimensional PAGE. While 2D Western blot analysis of both M. bovis sensitized and M. bovis infected cattle sera demonstrated an antibody boost following comparative intradermal TSTs, M. bovis sensitized cattle responded with greater intensity to additional SF-PPD antigens as compared to M. bovis infected cattle at seven weeks post infection/sensitization. In conclusion M. bovis sensitized cattle generated a more intense antibody response and recognized additional SF-PPD antigens as compared to M. bovis infected cattle within the first two months post infection/sensitization.

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Bouchez-Zacria, Malika. "Rôles de l’environnement et des contacts intra et interspécifiques dans la transmission de Mycobacterium bovis dans le système bovins-blaireaux en Pyrénées-Atlantiques – Landes". Thesis, Université Paris-Saclay (ComUE), 2018. http://www.theses.fr/2018SACLS552/document.

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En France, pays officiellement indemne de tuberculose bovine depuis 2001, l’infection persiste dans certaines zones, dont les Pyrénées-Atlantiques-Landes, où les niveaux d’infection observés chez les bovins et les blaireaux font craindre un fonctionnement particulier de ce système multi-hôtes pour Mycobacterium bovis, agent de la tuberculose bovine. L’objectif de cette thèse a été de mieux comprendre les mécanismes de transmission de M. bovis au sein du système bovins-blaireaux dans cette zone. L’analyse statistique du risque d’infection localement concomitante chez les blaireaux et les bovins a montré l’importance de variables environnementales liées à la survie de la bactérie dans le sol, telles que le microrelief et le pourcentage de sable dans le sol de surface, ainsi que celle de variables associées aux mouvements des blaireaux. L’analyse du réseau de contacts entre élevages bovins, incluant des contacts directs liés au commerce ou au voisinage au pâturage et des contacts indirects induits par un voisinage avec des domaines vitaux de blaireaux, a montré l’aspect multifactoriel de la transmission de M. bovis entre élevages. Enfin, la modélisation dynamique de la transmission de M. bovis dans le système bovins-blaireaux a suggéré une interface asymétrique entre blaireaux et bovins dans notre zone d’étude, les terriers étant pour la plupart infectés suite à l’exposition des blaireaux à une pâture dont le sol était contaminé par un bovin infectieux, alors que les modes de contamination des élevages se partageaient entre l’exposition des bovins à un élevage infectieux voisin de pâture, et la contamination de ces pâtures par un blaireau infectieux. La validation du modèle dynamique proposé doit encore être poursuivie. L’ensemble de nos résultats permet cependant de conclure à l’importance de prendre en compte tous les mécanismes de propagation dans la lutte contre la tuberculose bovine dans la zone. Le modèle développé est destiné à devenir un outil permettant de préciser les rôles épidémiologiques des bovins et des blaireaux et de simuler différentes stratégies de contrôle, pouvant inclure la vaccination des blaireaux
France is officially free from bovine tuberculosis since 2001, but this infection is still persistent in some areas, including Pyrénées-Atlantiques - Landes. There, both cattle and badgers were found infected, suggesting that this multi-host system might have a particular functioning regarding Mycobacterium bovis (the main agent for bovine tuberculosis in France) transmission. The objective of the thesis was to better understand M. bovis transmission mechanisms throughout the badger-cattle system in this area. The statistical analysis of the local risk of the badger and cattle concomitant infection showed the importance of environmental variables such as microrelief and sand proportion of the topsoil as well as variables depicting badger movements. The analysis of the contact network between farms (that included direct contacts due to trade and to neighbouring pastures and indirect contacts due to the vicinity with badger home ranges) showed a multifactorial aspect of the transmission between farms in our study area. Finally, the dynamic modelling of M. bovis transmission throughout the badger-cattle system suggested an asymmetric interface where the infection of badgers due to soil contaminated by infected cattle was predominant, whereas, for the infection of cattle, the soil contaminated by infected badgers had a similar importance as the presence of infectious cattle on the neighbouring pastures. Our dynamic model validation should be continued. Nevertheless, our whole range of results underlined the importance of targeting all transmission mechanisms for infection control. The model we proposed is a tool designated to assess both cattle and badgers’ epidemiological roles as well as to simulate different control strategies, that could include badgers’ vaccination

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8

Whiteford, Danelle. "Stress survival in Mycobacterium tuberculosis and Mycobacterium bovis and the role of hup in Mycobacterium smegmatis". Pullman, Wash. : Washington State University, 2008. http://www.dissertations.wsu.edu/Dissertations/Fall2008/D_Whiteford_100908.pdf.

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9

Young, Jamie Stuart. "Molecular detection of Mycobacterium bovis in the environment". Thesis, University of Warwick, 2003. http://wrap.warwick.ac.uk/47689/.

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An investigation was carried out to determine the presence and persistence of Mycobacterium bovis in the environment. Soil samples were taken in April 2000 from a farm in Ireland which had undergone a bovine tuberculosis outbreak some four months prior. Total community DNA was extracted from these samples and PCR carried out targeted to two genes specific for the Mycobacterium tuberculosis complex; mpb64 and mpb70. These genes were detected in soil samples taken from entrances to two badger sets and in soil from two sites where the infected cattle grazed. Further analysis of DNA using oligonucleotide primers specific for the 16S rRNA genes of slow-growing mycobacteria was carried out. This revealed the presence of 16S rRNA genes relating to Mycobacterium bovis. RT-PCR was also carried out using these primers on total community RNA. Sequences relating to M. bovis were found, indicating that the DNA and RNA came from viable, intact cells in the soil, and that M. bovis persists in soil for up to four months after contamination of the soil. Sampling was repeated in November 2002 following a second TB outbreak in January 2001. DNA sequences for mpb64 and mpb7O were only found in the samples from the badger setts, as were 16S rRNA sequences. The survival of the vaccine strain M. bovis BCG was also determined, using soil microcosms experiments in defined environmental conditions. M. bovis BCG was found to survive for longest at temperatures of 37°C and at soil wetting levels of 30%. Culturable cells could not be detected after 60 days, however DNA and RNA relating to M. bovis BCG could be detected up to 18 months after initial inoculation. This suggests the cells persisted in a viable non-culturable state. Experiments to determine the rate of persistence of DNA in soil were carried out. DNA was found to persist for no longer than 10 days in soil. Diversity studies were carried out on the farm samples and on Warwick soil, to determine the diversity of the mycobacterial population. 16S rRNA analysis was carried out and showed the presence ofsequences relating to M. bovis, Al. hiberniae, M. avium, Al. fallax, and M. farcinogenes.

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Sales, Erica Bravo. "Genotipagem de Mycobacterium bovis pelo multispacer sequence typing". Universidade Federal de Minas Gerais, 2012. http://hdl.handle.net/1843/BUOS-8YGH2X.

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Mycobacterium bovis, which causes bovine tuberculosis, is a species belonging to Mycobacterium tuberculosis complex. It shows 99.9% of genetic homology with other species of the complex, making the diagnosis difficult. The bovine bacilli represent a serious problem due to the direct losses in cattle, caused by such infirmity and possible sanitary barriers to exportation. Molecular typing, provide an innovative way to identify bacterial strains, trough generate while genotypes by combining different polymorphic DNA markers, contributing to their epidemiological investigation. The techniques currently used for typing of M. bovis are Spoligotyping and VNTR. However these methods are not able to recognize all genetics analyzed strains diversity such as sequencing methods based in last ones allow analyzing molecular target sequence, thereby identifying all events present in the genetic marker used. This paper aims standardize Multispacer Sequence Typing (MST) for M.bovis genotyping. Seven intergenic spacer region were analyzed on 58 M.bovis samples, coming from six brazilian's states (MG, GO, SP, MT, PR, RS), isolated between 2006 and 2010, and on four M.boviss reference strains. Four types of genetic events were observed: nucleotide mutation (SNP), insertion, deletion and tandem repeat (TR), totaling from the combination of genotypes obtained a total of 28 events. Twenty-eight type sequences (TS) have been also produced, showing a discrimination index of 93%, including the standard strain AF2122/97 [Genbank BX248333.1] used for comparison in silico. These data were used to analyze the pattern of evolutionary lineage of M. boviss isolates and correlate them with phylogeographic lineages, based on the formation of clonal complexes, generated from eBURST. This is the first study aimed identify the variability of isolates of M. bovis using MST method. Results were quite satisfactory. Method allowed us to typing and differentiate M.bovis isolate from a sequencing of few regions, even in restricted locations and in short a time as established in Bueno Brandao city.The method has the sequencing advantage and the availability of sequences analyzed in public databases which can be used by professionals around the world as an analyzes tool.
O Mycobacterium bovis, causador da tuberculose bovina, está entre as espécies pertencentes ao Complexo Mycobacterium tuberculosis. A doença causada pelo bacilo bovino constitui um grave problema devido aos prejuízos diretos causados por esta enfermidade e possíveis barreiras sanitárias na exportação. O genoma do M. bovis é >99,95% geneticamente idêntico ao de M. tuberculosis, dificultando o diagnóstico. A tipificação molecular permite inovar a identificação de uma espécie e ao mesmo tempo gerar genótipos mediante a combinação de diferentes marcadores polimórficos, contribuindo para a sua investigação epidemiológica. As técnicas mais utilizadas atualmente para tipificar o patógeno M. bovis são o Spoligotyping e o VNTR, no entanto, esses métodos não são capazes de reconhecer toda a diversidade genética dos isolados analisados como é o caso de métodos baseados em sequenciamento, que permitem a análise de sequências alvos moleculares, identificando dessa forma todos os eventos genéticos presentes no marcador utilizado. O objetivo deste trabalho foi tipificar isolados de Mycobacterium bovis pela técnica Multispacer Sequence Typing (MST). Sete regiões espaçadoras intergênicas foram analisadas para um total de 58 isolados de M. bovis relativos aos anos de 2006 a 2010, procedentes de fazendas localizadas em seis estados brasileiros (MG, GO, SP, MT, PR. RS) e para quatro amostras padrão de M. bovis. Quatro tipos de eventos genéticos foram observados: mutação de nucleotídeo único (SNP), inserção, deleção e repetição em tandem (TR), totalizando a partir da combinação dos genótipos obtidos um total de 28 eventos. Os resultados, obtidos pela comparação in silico entre os fragmentos gerados pelo sequenciamento e a cepa padrão M. bovis AF2122/97 [Genbank BX248333.1] permitiu identificar 28 perfis genotípicos únicos, caracterizando 28 sequências tipo (ST) na amostragem analisada e apontando um índice de discriminação de 93%. Esses dados foram utilizados para analisar os padrões de descendência evolutiva dos isolados de M. bovis e correlacioná-los a linhagens filogeográficas com base na formação de complexos clonais gerados a partir do eBURST. Este foi o primeiro trabalho a identificar a variabilidade dos isolados de M. bovis pelo método MST. Os resultados obtidos foram bastante satisfatórios, dado que o método permitiu tipificar e diferenciar isolados de M. bovis a partir do sequenciamento de poucas regiões espaçadoras, mesmo em localizações restritas e em um período de tempo curto como foi verificado no município de Bueno Brandão. O método apresenta a vantagem do sequenciamento e a disponibilização de sequências analisadas em bancos de dados públicos, que podem ser utilizadas por profissionais em todo o mundo como ferramenta para análises futuras.

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Più fonti

Libri sul tema "Mycobacterium bovis"

1

Bassey, Effiong Okon Etim. Studies on protein antigens of mycobacteria: A comparative study of "mycobacterium tuberculosis" and "mycobacterium bovis". Birmingham: University of Birmingham, 1990.

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O, Thoen Charles, Steele James H e Gilsdorf Michael J, a cura di. Mycobacterium bovis infection in animals and humans. 2^a ed. Ames, Iowa: Blackwell Pub., 2006.

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3

O, Thoen Charles, e Steele James H, a cura di. Mycobacterium bovis infection in animals and humans. Ames, Iowa: Iowa State University Press, 1995.

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4

Beck, Lesley Ann. Cloning, expression and purification of recombinant MPB83 antigen from mycobacterium bovis. (s.l: The Author), 1998.

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5

World Health Organization (WHO). Report of the WHO meeting on zoonotic tuberculosis (Mycobacterium bovis), with the participation of FAO, Geneva, 15 November, 1993. Geneva: World Health Organization, 1993.

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6

Stober, Carmel Bernadette. P2 purinoceptor involvement in ATP-mediated macrophage death and killing of intracellular Mycobacterium bovis bacille calmette-guérin. Birmingham: University of Birmingham, 2000.

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7

World Health Organization (WHO). Report of the WHO working group on zoonotic tuberculosis (Mycobacterium bovis), with the participationof the FAO, Mainz, Germany, 1994. Geneva: World Health Organization, 1994.

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8

Nolan, Ann. An investigation of the development of specific antibody responses of badgers (Meles meles) to infection with Mycobacterium bovis with reference to the pathogenesis and epidemiology of the disease. Uxbridge: Brunel University, 1991.

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9

Steele, James H., Charles O. Thoen e John B. Kaneene. Zoonotic Tuberculosis: Mycobacterium Bovis and Other Pathogenic Mycobacteria. Wiley & Sons, Limited, John, 2014.

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Steele, James H., Charles O. Thoen e John B. Kaneene. Zoonotic Tuberculosis: Mycobacterium Bovis and Other Pathogenic Mycobacteria. Wiley & Sons, Incorporated, John, 2014.

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Capitoli di libri sul tema "Mycobacterium bovis"

1

Bonamonte, Domenico, Angela Filoni e Gianni Angelini. "Mycobacterium bovis Skin Infection". In Mycobacterial Skin Infections, 127–40. Cham: Springer International Publishing, 2017. http://dx.doi.org/10.1007/978-3-319-48538-6_3.

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Skuce, Robin A., e Sydney D. Neill. "Molecular Epidemiology of Mycobacterium bovis". In Tuberculosis, 75–92. Berlin, Heidelberg: Springer Berlin Heidelberg, 2004. http://dx.doi.org/10.1007/978-3-642-18937-1_6.

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3

Rito, Teresa, Osvaldo Inlamea, Olena Oliveira, Raquel Duarte, Pedro Soares e Margarida Correia-Neves. "Evolution and Molecular Characteristics of Mycobacterium tuberculosis and Mycobacterium bovis". In Integrated Science, 847–65. Cham: Springer International Publishing, 2023. http://dx.doi.org/10.1007/978-3-031-15955-8_41.

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4

Muwonge, Adrian, Franklyn Egbe, Mark Bronsvoort, Demelash B. Areda, Tiny Hlokwe e Anita Michel. "Molecular Epidemiology of Mycobacterium bovis in Africa". In Tuberculosis in Animals: An African Perspective, 127–69. Cham: Springer International Publishing, 2019. http://dx.doi.org/10.1007/978-3-030-18690-6_8.

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5

Esteban, Jaime, e Maria-Carmen Muñoz-Egea. "Mycobacterium bovis and Other Uncommon Members of the Mycobacterium tuberculosis Complex". In Tuberculosis and Nontuberculous Mycobacterial Infections, 753–65. Washington, DC, USA: ASM Press, 2017. http://dx.doi.org/10.1128/9781555819866.ch44.

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Lahey, Timothy, e C. Fordham von Reyn. "Mycobacterium bovis BCG and New Vaccines for the Prevention of Tuberculosis". In Tuberculosis and Nontuberculous Mycobacterial Infections, 187–209. Washington, DC, USA: ASM Press, 2017. http://dx.doi.org/10.1128/9781555819866.ch11.

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Khattak, Irfan, Muhammad Hassan Mushtaq, Sultan Ayaz, Sajid Ali, Anwar Sheed, Javed Muhammad, Muhammad Luqman Sohail, Haq Amanullah, Irshad Ahmad e Sadeeq ur Rahman. "Incidence and Drug Resistance of Zoonotic Mycobacterium bovis Infection in Peshawar, Pakistan". In Advances in Experimental Medicine and Biology, 111–26. Cham: Springer International Publishing, 2018. http://dx.doi.org/10.1007/5584_2018_170.

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Grant, Irene R., e Linda D. Stewart. "Improved Detection of Mycobacterium bovis in Bovine Tissues Using Immunomagnetic Separation Approaches". In Veterinary Infection Biology: Molecular Diagnostics and High-Throughput Strategies, 153–61. New York, NY: Springer New York, 2014. http://dx.doi.org/10.1007/978-1-4939-2004-4_11.

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Cadmus, S. I. B., P. I. Fujiwara, J. A. Shere, B. Kaplan e C. O. Thoen. "The Control of Mycobacterium bovis Infections in Africa: A One Health Approach". In Tuberculosis in Animals: An African Perspective, 41–55. Cham: Springer International Publishing, 2019. http://dx.doi.org/10.1007/978-3-030-18690-6_4.

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10

Kotani, S., A. Nagao, T. Tamura, H. Okamura, A. Nagata, K. Aoyama, S. Kusumoto et al. "Purification and Endotoxin-Like Bioactivities of a Novel Amphiphile from Mycobacterium bovis BCG". In Immunotherapeutic Prospects of Infectious Diseases, 19–36. Berlin, Heidelberg: Springer Berlin Heidelberg, 1990. http://dx.doi.org/10.1007/978-3-642-76120-1_3.

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Atti di convegni sul tema "Mycobacterium bovis"

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Rimbu, Cristina Mihaela, Cristina Elena Horhogea, Catalin Carp-Carare, Dan Florin Chiriac, Gabriela Adriana Chiriac, Daniel Bejenariu, Danut Bratu e Mariana Caraman. "Aspecte privind epidemiologia speciilor Mycobacterium bovis și Mycobacterium caprae în județul Vaslui, Romania". In Scientific and practical conference with international participation: "Management of the genetic fund of animals – problems, solutions, outlooks". Scientific Practical Institute of Biotechnologies in Animal Husbandry and Veterinary Medicine, 2023. http://dx.doi.org/10.61562/mgfa2023.57.

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Mycobacterium bovis and Mycobacterium caprae are species that be-long to the Mycobacterium tuberculosis (CMT) complex, together with other species of va-rying clinical relevance, and are known to be the main causative agents of tuberculosis in animals and the primary causative agent of zoonotic tuberculosis. The aim of the study was to analyze the incidence indicators of Mycobacterium bovis and Mycobacterium caprae species in cattle from Vaslui County, Romania. The study included the analysis of data from 2015-2021, obtained as a result of microbiological examinations of various biological samples prelevated from cattle with suspected tuberculosis. With the exception of 2020, there were animals with suspected tuberculosis from which 115 strains of Myco-bacterium sp. were isolated throughout the 2015-2020 period. All strains were serotyped and classified into two species: Mycobacterium bovis (5,21%) and Mycobacterium caprae (94,78%). Analysis of the dispersion over time of Mycobacterium species isolation showed that Mycobacterium bovis was identified sporadically (2016, 2017), while Mycobacterium caprae was isolated continuously, except in 2020, when a number of sanitary-veterinary services were interrupted due to the crisis situation caused by the pandemic COVID -19.

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SYAHPUTRA, GITA. "Anti-mycobacterial activity of methanol plants extract to against Mycobacterium bovis and Mycobacterium smegmatis". In Seminar Nasional Masyarakat Biodiversitas Indonesia. Masyarakat Biodiversitas Indonesia, 2016. http://dx.doi.org/10.13057/psnmbi/m020211.

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3

Attig, F., SA Barth, M. Kohlbach, W. Baumgärtner e A. Lehmbecker. "Mycobacterium bovis-SB0950-Infektion bei einer Katze". In 62. Jahrestagung der Fachgruppe Pathologie der Deutschen Veterinärmedizinischen Gesellschaft. Georg Thieme Verlag KG, 2019. http://dx.doi.org/10.1055/s-0039-1688620.

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4

Sanville, B., e B. T. Kuhn. "Disseminated Mycobacterium Bovis After Bacillus-Calmette-Guerin Bladder Instillation". In American Thoracic Society 2019 International Conference, May 17-22, 2019 - Dallas, TX. American Thoracic Society, 2019. http://dx.doi.org/10.1164/ajrccm-conference.2019.199.1_meetingabstracts.a5114.

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Lee, Juhyun, Seoyoung Yoon e Taeseon Yoon. "Analysis of Mycobacterium Tuberculosis, Mycobacterium Bovis, and Mycobacterium Africanum that Cause Tuberculosis Using Apriori and Decision Tree Algorithm". In ICBBE 2017: 2017 4th International Conference on Biomedical and Bioinformatics Engineering. New York, NY, USA: ACM, 2017. http://dx.doi.org/10.1145/3168776.3168777.

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CARVALHO, RICARDO CÉSAR TAVARES, VINICIUS SILVA CASTRO, DANDARA VIRGINIA GUIA SEMEDO FERNANDES, GREIKA FERREIRA MOURA, ELIS CAROLINE CELESTINA SANTOS, VÂNIA MARGARET FLOSI PASCHOALIN e EDUARDO EUSTÁQUIO DE SOUZA FIGUEIREDO. "Use of the Pcr for Detection Mycobacterium Bovis in Milk". In XII Latin American Congress on Food Microbiology and Hygiene. São Paulo: Editora Edgard Blücher, 2014. http://dx.doi.org/10.5151/foodsci-microal-180.

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McLaughlin, Anne M., Joan Power, Susan Foley e Joseph M. Keane. "Primary Isoniazid Resistance In Mycobacterium Bovis Disease: A Disturbing Prospect". In American Thoracic Society 2012 International Conference, May 18-23, 2012 • San Francisco, California. American Thoracic Society, 2012. http://dx.doi.org/10.1164/ajrccm-conference.2012.185.1_meetingabstracts.a6747.

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Mason, Carol M., Elizabeth Porretta e Alistair Ramsay. "Alcohol Modulates The Immune Response To Murine Vaccination With Mycobacterium Bovis BCG". In American Thoracic Society 2010 International Conference, May 14-19, 2010 • New Orleans. American Thoracic Society, 2010. http://dx.doi.org/10.1164/ajrccm-conference.2010.181.1_meetingabstracts.a3223.

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Oliveira, L. B., I. D. C. Barreto, S. D. Vieira, M. E. Camargo e S. L. Russo. "PROSPECÇÃO TECNOLÓGICA SOBRE O CONTROLE E COMBATE DA TUBERCULOSE BOVINA (Mycobacterium bovis)". In 5th International Symposium on Technological Innovation. Universidade Federal do Sergipe, 2014. http://dx.doi.org/10.7198/s2318-3403201400020032.

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Jiang, Xiu-Yun, Chun-Fang Wang, Chun-Feng Wang, Fan-Li Zeng, Yu-Qing Hu e Zhao-Yang He. "Cloning and Expression of ESAT-6 Gene of Mycobacterium bovis in Escherichia coli". In 2009 3rd International Conference on Bioinformatics and Biomedical Engineering (iCBBE). IEEE, 2009. http://dx.doi.org/10.1109/icbbe.2009.5162511.

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Rapporti di organizzazioni sul tema "Mycobacterium bovis"

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Nelson, Corwin D., Donald C. Beitz, Timothy A. Reinhardt e John D. Lippolis. Regulation of Immune Responses to Mycobacteria bovis by a Paracrine Mechanism of Vitamin D Signaling in Cattle. Ames (Iowa): Iowa State University, gennaio 2011. http://dx.doi.org/10.31274/ans_air-180814-646.

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