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Letteratura scientifica selezionata sul tema "Microfluidic sorting"

Autore: Grafiati

Pubblicato: 1 marzo 2025

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Articoli di riviste sul tema "Microfluidic sorting"

1

Fan, Dan, Yi Liu e Yaling Liu. "The Latest Advances in Microfluidic DLD Cell Sorting Technology: The Optimization of Channel Design". Biosensors 15, n. 2 (19 febbraio 2025): 126. https://doi.org/10.3390/bios15020126.

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Cell sorting plays a crucial role in both medical and biological research. As a key passive sorting technique in the field of microfluidics, deterministic lateral displacement (DLD) has been widely applied to cell separation and sorting. This review aims to summarize the latest advances in the optimization of channel design for microfluidic DLD cell sorting. First, we provide an overview of the design elements of microfluidic DLD cell sorting channels, focusing on key factors that affect separation efficiency and accuracy, including channel geometry, fluid dynamics, and the interaction between cells and channel surfaces. Subsequently, we review recent innovations and progress in channel design for microfluidic DLD technology, exploring its applications in biomedical fields and its integration with machine learning. Additionally, we discuss the challenges currently faced in optimizing channel design for microfluidic DLD cell sorting. Finally, based on existing research, we make a summary and put forward prospective views on the further development of this field.

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Khodamoradi, Maedeh, Saeed Rafizadeh Tafti, Seyed Ali Mousavi Shaegh, Behrouz Aflatoonian, Mostafa Azimzadeh e Patricia Khashayar. "Recent Microfluidic Innovations for Sperm Sorting". Chemosensors 9, n. 6 (1 giugno 2021): 126. http://dx.doi.org/10.3390/chemosensors9060126.

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Sperm selection is a clinical need for guided fertilization in men with low-quality semen. In this regard, microfluidics can provide an enabling platform for the precise manipulation and separation of high-quality sperm cells through applying various stimuli, including chemical agents, mechanical forces, and thermal gradients. In addition, microfluidic platforms can help to guide sperms and oocytes for controlled in vitro fertilization or sperm sorting using both passive and active methods. Herein, we present a detailed review of the use of various microfluidic methods for sorting and categorizing sperms for different applications. The advantages and disadvantages of each method are further discussed and future perspectives in the field are given.

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3

Zhang, Yueyue, Tingting Zheng, Li Wang, Liang Feng, Min Wang, Zhenchao Zhang e Huanhuan Feng. "From passive to active sorting in microfluidics: A review". REVIEWS ON ADVANCED MATERIALS SCIENCE 60, n. 1 (1 gennaio 2021): 313–24. http://dx.doi.org/10.1515/rams-2020-0044.

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Abstract Achieving high-efficiency sorting of microfluidics (such as cells, particles, droplets, etc.) has great significance in the fields of biology, chemistry, medical treatment, material synthesis, and drug development. This paper introduces the microfluidics sorting methods in recent years. The current research status and progress can be divided into the active sorting system and passive sorting system according to whether there is an external field. They can control the microfluidics by promoting more selective separation, so as to obtain higher resolution and selection rate. In this paper, the above methods are analyzed and discussed, and the future microfluidic sorting is prospected.

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4

Yang, He, e Tuomas P. J. Knowles. "Hydrodynamics of Droplet Sorting in Asymmetric Acute Junctions". Micromachines 13, n. 10 (29 settembre 2022): 1640. http://dx.doi.org/10.3390/mi13101640.

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Droplet sorting is one of the fundamental manipulations of droplet-based microfluidics. Although many sorting methods have already been proposed, there is still a demand to develop new sorting methods for various applications of droplet-based microfluidics. This work presents numerical investigations on droplet sorting with asymmetric acute junctions. It is found that the asymmetric acute junctions could achieve volume-based sorting and velocity-based sorting. The pressure distributions in the asymmetric junctions are discussed to reveal the physical mechanism behind the droplet sorting. The dependence of the droplet sorting on the droplet volume, velocity, and junction angle is explored. The possibility of the employment of the proposed sorting method in most real experiments is also discussed. This work provides a new, simple, and cost-effective passive strategy to separate droplets in microfluidic channels. Moreover, the proposed acute junctions could be used in combination with other sorting methods, which may boost more opportunities to sort droplets.

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Chiu, Yi-Lung, Ruchi Ashok Kumar Yadav, Hong-Yuan Huang, Yi-Wen Wang e Da-Jeng Yao. "Unveiling the Potential of Droplet Generation, Sorting, Expansion, and Restoration in Microfluidic Biochips". Micromachines 10, n. 11 (6 novembre 2019): 756. http://dx.doi.org/10.3390/mi10110756.

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Microfluidic biochip techniques are prominently replacing conventional biochemical analyzers by the integration of all functions necessary for biochemical analysis using microfluidics. The microfluidics of droplets offer exquisite control over the size of microliter samples to satisfy the requirements of embryo culture, which might involve a size ranging from picoliter to nanoliter. Polydimethylsiloxane (PDMS) is the mainstream material for the fabrication of microfluidic devices due to its excellent biocompatibility and simplicity of fabrication. Herein, we developed a microfluidic biomedical chip on a PDMS substrate that integrated four key functions—generation of a droplet of an emulsion, sorting, expansion and restoration, which were employed in a mouse embryo system to assess reproductive medicine. The main channel of the designed chip had width of 1200 μm and height of 500 μm. The designed microfluidic chips possessed six sections—cleaved into three inlets and three outlets—to study the key functions with five-day embryo culture. The control part of the experiment was conducted with polystyrene (PS) beads (100 μm), the same size as the murine embryos, for the purpose of testing. The outcomes of our work illustrate that the rate of success of the static droplet culture group (87.5%) is only slightly less than that of a conventional group (95%). It clearly demonstrates that a droplet-based microfluidic system can produce a droplet in a volume range from picoliter to nanoliter.

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Buschke, D. G., P. Resto, N. Schumacher, B. Cox, A. Tallavajhula, A. Vivekanandan, K. W. Eliceiri, J. C. Williams e B. M. Ogle. "Microfluidic sorting of microtissues". Biomicrofluidics 6, n. 1 (marzo 2012): 014116. http://dx.doi.org/10.1063/1.3692765.

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7

Catarino, Susana O., Raquel O. Rodrigues, Diana Pinho, João M. Miranda, Graça Minas e Rui Lima. "Blood Cells Separation and Sorting Techniques of Passive Microfluidic Devices: From Fabrication to Applications". Micromachines 10, n. 9 (10 settembre 2019): 593. http://dx.doi.org/10.3390/mi10090593.

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Since the first microfluidic device was developed more than three decades ago, microfluidics is seen as a technology that exhibits unique features to provide a significant change in the way that modern biology is performed. Blood and blood cells are recognized as important biomarkers of many diseases. Taken advantage of microfluidics assets, changes on blood cell physicochemical properties can be used for fast and accurate clinical diagnosis. In this review, an overview of the microfabrication techniques is given, especially for biomedical applications, as well as a synopsis of some design considerations regarding microfluidic devices. The blood cells separation and sorting techniques were also reviewed, highlighting the main achievements and breakthroughs in the last decades.

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Wang, Xiao, Xiaodi Yang e Ian Papautsky. "An integrated inertial microfluidic vortex sorter for tunable sorting and purification of cells". TECHNOLOGY 04, n. 02 (giugno 2016): 88–97. http://dx.doi.org/10.1142/s2339547816400112.

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Sorting of target cells from complex cellular samples into a high-purity product is challenging yet essential for downstream cell biology research and clinical diagnostics. Inertial microfluidics is an emerging technology attracting a lot of interest for passive and label-free sorting of cells with high throughput. Here, we introduce an inertial microfluidic device based on our vortex sorting platform for continuous size-based double sorting and purification of the larger target cells from the smaller background cells. Our device uses a microscale chamber with three outlets as a sorting unit, and integrates it into a specific topology to enable double sorting and purification functionalities. With properly designed fluidic resistance network and optimized flow conditions, we demonstrated continuous sorting of spiked human cancer stem-like cells from human blood with >90% efficiency and >1,500× enhanced purity, as well as removal of red blood cells with ~99.97% efficiency. We envision this integrated vortex-aided sorter can serve as a viable tool for size-based sorting of large target cells from complex cellular samples. Furthermore, this vortex-aided sorting platform can be integrated into more sophisticated topology with versatile functions for other cell sorting applications.

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Zhu, Guiping, e Nam Trung Nguyen. "Particle Sorting in Microfluidic Systems". Micro and Nanosystemse 2, n. 3 (1 settembre 2010): 202–16. http://dx.doi.org/10.2174/1876402911002030202.

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10

Xue, Xinyue, Hongjun Ye e Zuocheng Hu. "Microfluidic System for Cell Sorting". Journal of Physics: Conference Series 2012, n. 1 (1 settembre 2021): 012129. http://dx.doi.org/10.1088/1742-6596/2012/1/012129.

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Tesi sul tema "Microfluidic sorting"

1

Gao, Hua. "Microluidic Sorting of Blood Cells by Negative Selection". University of Cincinnati / OhioLINK, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1479816171280535.

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2

shahzad, mohd adnan faqui. "Microfluidic Chip development for acoustophoresis assisted selective cell sorting". Thesis, KTH, Skolan för kemi, bioteknologi och hälsa (CBH), 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-223658.

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Analysis of blood samples is one of the major steps in diagnosing pathological conditions like cancer. The upstream sample preparation for the pathological cell analysis from complex biological fluid like blood, involves selective cell sorting. It can be achieved using fluorescently activated or magnetically activated cell sorters. Another way is to sort them using acoustophoresis which is cheaper, gives better spatial control and is also rapid apart from the fact that, it does not affect the cellular viability.6,9 In acoustophoresis, particles depending upon their density and compressibility relative to the suspended medium migrate to either pressure anti-nodes or nodes, when subjected to acoustic field. Poly vinyl alcohol-based microbubbles have a strong negative acoustic contrast factor and hence migrate to the anti-nodes in a standing ultrasonic wave. Previously, this property was utilized for cell separation by conjugating the bubbles to cells and subjecting them to ultrasonic waves in a silicon glass based microfluidic channel.55 A protocol for coating the microbubbles with avidin, so that these can readily attach to the cells has been developed in this work. However, microfluidic channel is obtained from a master mold which is developed in a clean room facility using photolithography. A cost-effective way has been developed for the production of a mold using a Computerized Numerical Control system (where the positive master for the microfluidic channel is drilled onto a PMMA sheet) for continuous separation of cancer cells. Alternate methods like a cutting plotter (which uses a double sided adhesive tape as a positive master) and a 3-D printer have been investigated, in order to be used as a mold for the microfluidic channel. As a proof, microbubbles-cell complex was focused in a PDMS based microfluidic channel, by utilizing standing Bulk acoustic waves. At flow rate of 10µl/min, efficiency greater than 80% has been achieved. This technique is low cost and can be implemented in places without a clean room facility for size independent cell sorting.

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Seo, Duckbong. "A development of the motile sperm sorting microfluidic devices". Diss., Columbia, Mo. : University of Missouri-Columbia, 2007. http://hdl.handle.net/10355/4798.

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Thesis (Ph. D.)--University of Missouri-Columbia, 2007.
The entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file. Title from title screen of research.pdf file (viewed on December 13, 2007) Vita. Includes bibliographical references.

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Raafat, Mohamed Salem. "Self-sorting of deformable particles in a microfluidic circuit". Thesis, Massachusetts Institute of Technology, 2010. http://hdl.handle.net/1721.1/62536.

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Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2010.
Cataloged from PDF version of thesis.
Includes bibliographical references (p. 54-57).
In this thesis, a new microfluidic device is presented for sorting of deformable particles based on the hydrodynamic resistance induced in a microchannel. Hydrodynamic resistance can be related to physical properties, including size and deformability of the particle, and can also be influenced by particle-wall interactions, hence allowing sorting based on any of these characteristics. This device could find application in cell sorting and bioseparation for therapeutics, research, and point-of-care diagnostics, as well as in sorting of droplets and emulsions for research and industrial applications (e.g., pharmaceutics, food industry, etc.). The device design is carried out using an equivalent resistance model, and numerical simulations are used to validate the design. The device is fabricated in PDMS, flow velocities are characterized using particle streak velocimetry, and sorting experiments are conducted to sort deformable gelatin particles according to size, and droplets of water and glycerol according to deformability. A sorting resolution of approximately 1 pm was obtained when sorting based on size, and droplets of water and glycerol were sorted into separate streams when sorting based on deformability. The main strength of the device over existing technology lies in its simplicity: sorting is carried out passively in the microfluidic circuit, eliminating the need for additional detection or sorting modules. Moreover, the device could be easily customized to change the sorting parameter or the sorting threshold, and multiple devices can be combined in parallel (to increase throughput) or in series (to increase resolution).
by Mohamed Salem Raafat.
S.M.

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5

Haener, Edgar. "Microfluidic segregation of capsules". Thesis, University of Manchester, 2017. https://www.research.manchester.ac.uk/portal/en/theses/microfluidic-segregation-of-capsules(a7e001f1-536c-475d-83d5-82aaa4098f5b).html.

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This thesis investigates the transport and sorting of capsules (elastic membranes enclosing a liquid core) using viscous flow in complex vessel geometries. Of particular interest is passive sorting by deformability using only the fluid-structure interaction between the capsule, the viscous fluid and the geometry of the vessel. Millimetric alginate-ovalbumin capsules in the regime of negligible fluid inertia are used in this work. In order to characterise the elastic properties of the capsules, a novel numerical finite element model of the compression of a thick-shelled capsule between parallel plates is implemented. The constitutive model of the capsule membranes was determined by comparison to experimental data: a Yeoh constitutive model with the ratio of constants $C_1 = 1$, $C_2 = 0$ and $C_3 = 10$ describes the capsules used. Three geometries are investigated in this work. (i) A T-Junction bifurcation. Capsule deformation in the T-Junction bifurcation is characterised by the maximal length of the capsule $L_{max}$ and depends on the ratio of viscous to elastic forces, the capillary number $Ca$. The maximal length, $L_{max}$, is especially sensitive at distinguishing soft capsules by their deformability. The sensitivity of $L_{max}$ to capsule compliance and the large deformations that can be achieved makes the T-junction a promising geometry in which to measure elastic properties of the capsules. The rate of relaxation of the capsules after the bifurcation is independent of their deformation. (ii) A half-cylinder obstacle in a channel followed by a sudden expansion. We show that the half-cylinder obstacle causes capsule trajectories to vary depending on deformability. Capsules with a factor of three difference in deformability can be separated. A practical feature of the system is its relative insensitivity to the initial lateral position of the capsules in the channel. However, while the results are reproducible across different capsules, the variations in final position amount to 10 \% at fixed parameters. As these experiments were conducted with the same capsule under identical flow conditions, this is likely to represent the best case scenario. (iii) We adapt the pinched flow fractionation (PFF) geometry to the sorting of capsules. We show that the standard PFF device cannot be used to sort capsules. However, a novel mode of operation, termed the ``T-Junction'' mode, shows great promise for the sorting of capsules. The PFF device in the T-Junction mode separates capsules with a factor of 1.5 difference in deformability. This is twice as sensitive as the half-cylinder device, although larger variability was observed in the PFF device.

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Gerhardt, Antimony L. "Arrayed microfluidic actuation for active sorting of fluid bed particulates". Thesis, Massachusetts Institute of Technology, 2003. http://hdl.handle.net/1721.1/37198.

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Thesis (M. Eng.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, February 2004.
Includes bibliographical references (p. 227-237).
Fluidic actuation offers a facile method to move large quantities of small solids, often referred to as fluid-bed movement. Applications for fluid bed processing are integral to many fields including petrochemical, petroleum, chemical, pharmaceutical, biochemical, environmental, defense, and medical. Thermal vapor microbubbles have been shown to be a low power input with high work output fluidic actuation technique with demonstrated commercial applications in ink jet printing and optical switching. This thesis further develops microbubble actuation (BA) as an arrayed particulate actuation technology for active sorting in particulate fluid beds. Numerical and analytical models of flows, forces, and fields affecting a tBA-based system are presented. The design and fabrication of an arrayed pBA-powered device are delineated with notation of specifications that may focus future design iterations. Performance testing and characterization of CpBA technology, including over a hundred in-plane and out-of-plane nucleation site geometries, serve as the impetus for the technical guidelines that are presented, which include a detailed comparison of in-plane and out-of-plane nucleation site geometry performance.
by Antimony L. Gerhardt.
M.Eng.

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Cartas, Ayala Marco Aurelio. "Hydrodynamic resistance and sorting of deformable particles in microfluidic circuits". Thesis, Massachusetts Institute of Technology, 2013. http://hdl.handle.net/1721.1/79312.

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Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2013.
Cataloged from PDF version of thesis.
Includes bibliographical references.
Sorting of microparticles has numerous applications in science and technology, from cell analysis to sample purification for biomaterials, photonics, and drug delivery. Methods used for particle separation relied only on procedures that involved sedimentation, filtration through porous material or other physical procedures that could be performed macroscopically and in bulk; only recently has miniaturization of fluid systems enabled individual particle separation at the macroscopic level. In the 1980's, as new fabrication techniques originally used to miniaturize circuits became available, they were used to miniaturize structures used for filtration, creating new membranes for filtration with sub millimeter thickness and new fluidic devices that enabled completely new functionalities. Hydrodynamic resistance, the extra resistance induced by a particle as it flows through a microfluidic channel, has been recently proposed as a viable property for particle characterization. Particle-induced hydrodynamic resistance can be linked to relevant biological properties, e.g. deformability, which is an important parameter in diseases like sickle cell anemia, malaria, sepsis and some kinds of cancers. In this work we propose the concept of 'hydrodynamic resistance sorting', which adds to the repertoire of current sorting technologies. We propose a microfluidic circuit capable of sorting particles according to the hydrodynamic resistance they induce in micro channel as they flow through. The circuit has two flow modes: rejection and sorting modes. The microfluidic circuit switches from rejection to sorting mode automatically when a particle induces an increment in hydrodynamic resistance larger than a designed threshold value. The circuit uses the concept of microfluidic logic, in which a microfluidic system has multiple discrete output modes, (sorting and rejecting particle modes), which are activated by an input variable, in this case the hydrodynamic resistance. As opposed to previous logic microfluidic circuits based on droplets, the sorting circuit uses particle self-interactions and does not require particle synchronization to enable microfluidic logic; hence the circuit is asynchronous. Further, we showed the circuit's ability to work with cells by sorting red blood cells and tested the circuit's capacity to sort particles based on mechanical properties by sorting cured and uncured droplets made of a UV-curable solution. Finally, in addition to development of circuits to sort particles based on hydrodynamic resistance, we investigated the link between hydrodynamic resistance and the change in mechanical properties experienced by cells. From first principles it is unclear exactly how and to what extent cell mechanical properties affect cell passage through constrained channels. The force opposing cell passage could be proportional to the cell velocity, as it occurs during lubrication of rigid objects, or proportional to normal forces, as it occurs in the case of many macroscopic objects sliding on surfaces. We used a microfluidic differential manometer, particle image velocimetry, high-speed imaging, confocal microscopy and non-dimensional analysis to investigate the relationship between cell mechanical properties, friction forces and hydrodynamic resistance. The results revealed that the transport of cells through constrained channels is a soft lubrication flow, where the driving force depends primarily on viscous dissipation and secondarily on the compressive forces acting on the cell. This work advances our understanding of the flow of deformable particles through constrained channels and provides a method to sort single particles based on their hydrodynamic resistance. The devices developed here have potential applications in biomechanical analysis of cells, bioseparation, point-of-care diagnostics, as well as in two-phase microfluidics.
by Marco Aurelio Cartas Ayala.
Ph.D.

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Aubrecht, Donald Michael. "Droplet Microfluidics: Tools for Screening and Sorting Applications". Thesis, Harvard University, 2013. http://dissertations.umi.com/gsas.harvard:11069.

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Microfluidic droplets are a powerful tool for screening large populations of cells, molecules, and biochemical reactions. Droplet systems are able to encapsulate, incubate, screen, and sort millions of samples, providing access to large number statistics that make searching for rare events feasible. Initial development of the microfluidic devices and methods has attracted applications in biology, biochemistry, and material science, but the set of tools remains incomplete. Efforts are required to develop micro-scale droplet analogs for all bulk-scale bench top procedures and instruments. The droplet analogs must be versatile, robust, and process samples rapidly.
Engineering and Applied Sciences

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Riordon, Jason A. "Developing Microfluidic Volume Sensors for Cell Sorting and Cell Growth Monitoring". Thèse, Université d'Ottawa / University of Ottawa, 2014. http://hdl.handle.net/10393/30955.

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Microfluidics has seen an explosion in growth in the past few years, providing researchers with new and exciting lab-on-chip platforms with which to perform a wide variety of biological and biochemical experiments. In this work, a volume quantification tool is developed, demonstrating the ability to measure the volume of individual cells at high resolution and while enabling microfluidic sample manipulations. Care is taken to maximise measurement sensitivity, range and accuracy, though novel use of buoyancy and dynamically tunable microchannels. This first demonstration of a microfluidic tunable volume sensor meant volume sensing over a much wider range, enabling the detection of ̴ 1 µm3 E.coli that would otherwise go undetected. Software was written that enables pressure-driven flow control on the scale of individual cells, which is used to great success in (a) sorting cells based on size measurement and (b) monitoring the growth of cells. While there are a number of macroscopic techniques capable of sorting cells, microscopic lab-on-chip equivalents have only recently started to emerge. In this work, a label-free, volume sensor operating at high resolution is used in conjunction with pressure-driven flow control to actively extract particle/cell subpopulations. Next, a microfluidic growth monitoring device is demonstrated, whereby a cell is flowed back and forth through a volume sensor. The integration of sieve valves allows cell media to be quickly exchanged. The combination of dynamic trapping and rapid media exchange is an important technological contribution to the field, one that opens the door to studies focusing on cell volumetric response to drugs and environmental stimuli. This technology was designed and fabricated in-house using soft lithography techniques readily available in most biotechnology labs. The main thesis body contains four scientific articles that detail this work (Chapters 2-5), all published in peer-reviewed scientific journals. These are preceded by an introductory chapter which provides an overview to the theory underlying this work, in particular the non-intuitive physics at the microscale and the Coulter principle.

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Xavier, Miguel. "Label-free, microfluidic characterisation and sorting of human skeletal stem cells". Thesis, University of Southampton, 2018. https://eprints.soton.ac.uk/424494/.

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Skeletal stem cells (SSCs) are a sub-population of bone marrow (BM) stromal cells with multipotent differentiation potential. SSCs are responsible for the unique regeneration capacity inherent to bone and offer unlimited potential for application in bone regenerative therapies. A current unmet challenge hampering their clinical translation remains the isolation of homogeneous SSC populations with consistent regeneration and differentiation capacities. Factors limiting the efficiency of existing sorting approaches include the scarcity of SSCs in BM, estimated at fewer than 1 in 10,000 nucleated cells, the complexity of BM tissue and, most significantly, the absence of a specific marker that is unique to the SSC. Microfluidics offers the potential to characterise and sort cells marker-free, based on intrinsic biophysical properties. These include, but are not limited to, cell size, shape, stiffness, and dielectric properties. The work presented herein aimed to provide a comprehensive characterisation of the biophysical fingerprint of SSCs and to build on this new understanding to develop new tools to isolate SSCs, label-free, with significant physiological and therapeutic implications. In real-time deformability cytometry (RT-DC), cells are deformed by shear and normal stresses as they flow through a narrow constriction at high speed, providing the capability to screen cell mechanical properties at high-throughput. Here, RT -DC was used to relate the mechano-phenotype of expanded SSCs with other cells in BM. Critically, SSCs were found to be significantly stiffer than white blood cells, which are abundant in human BM. Microfluidic impedance cytometry was coupled to fluorescence optical detection to provide accurate characterisation of the dielectric properties and cell size of SSCs within heterogeneous primary human BM samples. The membrane capacitance of SSCs was found to be indistinct from other cells in BM. Conversely, their average size in suspension, at 9 micrometres, was within the largest BM cell fraction. Centred on these findings, label-free sorting devices were designed based on the principle of deterministic lateral displacement (DLD). DLD uses arrays of micropillars in a channel to sort cells based on their diameter, at throughputs of thousands per second. Cell deformation, induced by shear and contact with the pillars, can change the effective cell size and affect sorting efficiency. This was demonstrated using two human cells lines of different size and stiffness, and by size fractionation of expanded SSCs. Crucially, SSCs sorted by DLD remained viable and retained their capacity to form clonogenic cultures. Overall, this work provided a detailed characterisation of relevant biophysical properties of SSCs and paved the way towards the design of a novel label-free sorting approach, potentially based on DLD, to provide purified SSC populations from BM with impactful use in fundamental stem cell research and the clinic.

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Più fonti

Libri sul tema "Microfluidic sorting"

1

Microfluidic Concentration Gradient Generation and Integrated Magnetic Sorting of Microparticles. [New York, N.Y.?]: [publisher not identified], 2013.

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Capitoli di libri sul tema "Microfluidic sorting"

1

Telleman, Pieter, Ulrik Darling Larsen, John Philip, Gert Blankenstein e Anders Wolff. "Cell Sorting in Microfluidic Systems". In Micro Total Analysis Systems ’98, 39–44. Dordrecht: Springer Netherlands, 1998. http://dx.doi.org/10.1007/978-94-011-5286-0_9.

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Lee, Wonhee, Peter Tseng e Dino Di Carlo. "Microfluidic Cell Sorting and Separation Technology". In Microsystems and Nanosystems, 1–14. Cham: Springer International Publishing, 2016. http://dx.doi.org/10.1007/978-3-319-44139-9_1.

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Guo, Quan, Simon P. Duffy e Hongshen Ma. "Microfluidic Technologies for Deformability-Based Cell Sorting". In Microsystems and Nanosystems, 225–54. Cham: Springer International Publishing, 2016. http://dx.doi.org/10.1007/978-3-319-44139-9_8.

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Yata, Vinod Kumar. "Microfluidic and Non-microfluidic Methods of Sperm Sorting and Sperm Analysis". In Microfluidics for Assisted Reproduction in Animals, 35–50. Singapore: Springer Singapore, 2021. http://dx.doi.org/10.1007/978-981-33-4876-9_3.

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Au, Sam H. "Circulating Tumor Cell Cluster Sorting by Size and Asymmetry". In Microfluidic Systems for Cancer Diagnosis, 15–23. New York, NY: Springer US, 2023. http://dx.doi.org/10.1007/978-1-0716-3271-0_2.

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Wei, Huibin. "Microfluidic Device with Integrated Porous Membrane for Cell Sorting and Separation". In Springer Theses, 61–82. Berlin, Heidelberg: Springer Berlin Heidelberg, 2012. http://dx.doi.org/10.1007/978-3-642-32359-1_4.

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Barrett, Louise M., e Blake A. Simmons. "Cell Sorting". In Encyclopedia of Microfluidics and Nanofluidics, 346–59. New York, NY: Springer New York, 2015. http://dx.doi.org/10.1007/978-1-4614-5491-5_199.

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Barrett, Louise M., e Blake A. Simmons. "Cell Sorting". In Encyclopedia of Microfluidics and Nanofluidics, 1–15. Boston, MA: Springer US, 2014. http://dx.doi.org/10.1007/978-3-642-27758-0_199-2.

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Chien, Yu Sheng, Che Hsin Lin, Fu Jen Kao e Cheng Wen Ko. "A Fully Integrated System for Cell/Particle Sorting in a Microfluidic Device Utilizing an Optical Tweezing and DIP Recognition Approach". In Materials Science Forum, 643–48. Stafa: Trans Tech Publications Ltd., 2006. http://dx.doi.org/10.4028/0-87849-990-3.643.

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Descamps, Lucie, Emmanuelle Laurenceau, Sophie Cavassila, Léa Payen, Damien Le Roy e Anne-Laure Deman. "Microchip for Immunomagnetic Sorting of Circulating Tumor Cells (CTCs)". In Microfluidics Diagnostics, 91–100. New York, NY: Springer US, 2024. http://dx.doi.org/10.1007/978-1-0716-3850-7_5.

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Atti di convegni sul tema "Microfluidic sorting"

1

Wang, Yan-Xiong, Jun-Shuai Wu, Wen-Yi Zhu, Wen Jiang, Yan-Feng Jiang e Tian Qiang. "Integrated Dual-Band Microwave Resonant Sensor With Microfluidic Sorting Chip for Biological Cell Detection". In 2024 IEEE International Symposium on Radio-Frequency Integration Technology (RFIT), 1–3. IEEE, 2024. https://doi.org/10.1109/rfit60557.2024.10812538.

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Ježek, Jan, Zdeněk Pilát, Mojmír Šerý, Jan Kaňka, Ota Samek, Silva Bernatová e Pavel Zemánek. "Microfluidic systems for optical sorting". In 18th Czech-Polish-Slovak Optical Conference on Wave and Quantum Aspects of Contemporary Optics, a cura di Jan Peřina, Libor Nozka, Miroslav Hrabovský, Dagmar Senderáková, Waclaw Urbańczyk e Ondrej Haderka. SPIE, 2012. http://dx.doi.org/10.1117/12.2008649.

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Tsai, Scott S. H., e Howard A. Stone. "Microfluidic Magnetic Multi-Cell Sorting". In ASME 2010 8th International Conference on Nanochannels, Microchannels, and Minichannels collocated with 3rd Joint US-European Fluids Engineering Summer Meeting. ASMEDC, 2010. http://dx.doi.org/10.1115/fedsm-icnmm2010-30269.

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We propose a cell sorting system that uses permanent magnets in a microfluidic device. Functionalized magnetic beads attached to cells and take on different trajectories based on the magnetic forces acting on them.

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Blomdahl, Jacob, Aaron Putzke e Philip Measor. "A 3D printed microfluidic particle sorting device". In Microfluidics, BioMEMS, and Medical Microsystems XXII, a cura di Bonnie L. Gray, Bastian E. Rapp e Colin Dalton. SPIE, 2024. http://dx.doi.org/10.1117/12.3002010.

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Qiang, Yuhao, Jia Liu, Darryl Dieujuste, Katrina Ramsamooj e Sarah E. Du. "Continuous Cell Sorting by Dielectrophoresis in a Straight Microfluidic Channel". In ASME 2018 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 2018. http://dx.doi.org/10.1115/imece2018-88156.

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Dielectrophoresis (DEP) has been demonstrated as an effective mechanism for cell sorting in microfluidic settings. Many existing methods utilize sophisticated microfluidic designs that require complicated fabrication process and operations. In this paper, we present a microfluidics-based cell sorter that is capable of sorting microparticles continuously in a simple straight channel, thus facilitating easier fabrication and operation. An array of indium-tin oxide (ITO) electrodes are embedded on the bottom surface of the straight channel to generate a DEP force field. This force results in deviation of the particles with different dielectric properties from their paths that are hydrodynamically focused in the channel. Particle trajectories are predicted by numerical simulation at different flow rates and field strengths using COMSOL. Separation of red blood cells from polystyrene beads is demonstrated and numerical prediction is validated experimentally. High separation efficiency for the two particle types is confirmed by counting the concentrations of particles collected at the respective collection outlet.

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Malik, Sarul, Prerna Balyan, J. Akhtar e Ajay Agarwal. "Microfluidic-chip platform for cell sorting". In 5TH NATIONAL CONFERENCE ON THERMOPHYSICAL PROPERTIES: (NCTP‐09). American Institute of Physics, 2016. http://dx.doi.org/10.1063/1.4945158.

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Shihya Hung, Chia-Hsien Hsu e Chihchen Chen. "Cell sorting in microfluidic systems using dielectrophoresis". In 2015 IEEE 15th International Conference on Nanotechnology (IEEE-NANO). IEEE, 2015. http://dx.doi.org/10.1109/nano.2015.7388752.

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Karunanidhi, Shrutilaya, Michael W. Lum, Shravya R. Nagurla e William C. Tang. "Microfluidic platforms for size-based cell sorting". In 2013 IEEE 7th International Conference on Nano/Molecular Medicine and Engnieering (NANOMED). IEEE, 2013. http://dx.doi.org/10.1109/nanomed.2013.6766310.

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Taylor, Jay K., Carolyn L. Ren e G. D. Stubley. "Numerical and Microfluidic-Based Cell-Sorting Devices". In ASME 2007 International Mechanical Engineering Congress and Exposition. ASMEDC, 2007. http://dx.doi.org/10.1115/imece2007-41329.

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The development of Lab-on-a-Chip devices with integrated bio-analysis functions requires a complex network of microfluidic transport and processes. Many of these functions call for the isolation or separation of specific bio-particles or cells. The design of a miniaturized cell-sorting device for handheld operation must follow the strict parameters associated with Lab-on-a-Chip technology. The limitations include applied voltage, high efficiency of cell-separation, repeatability, size, flow control, and cost, among others. Currently used designs have achieved successful levels of cell-isolation. However, further improvements in the microfluidic chip design are important for incorporation into larger systems. This study evaluates specific design modifications that contribute to the reduction of required applied potential aiming for developing portable devices, improved operation reliability by minimizing induced pressure disturbance when electrokinetic pumping is employed and incorporating online filters to reduce channel blockage, and improved flow control by incorporating directing streams achieving dynamic sorting and counting. The chip designs fabricated in glass and polymeric materials include asymmetric channel widths for sample focusing, nonuniform channel depth for minimizing induced pressure disturbance, directing streams to assist particle flow control, and online filters for reducing channel blockage. Fluorescence-based visualization of electrokinetic focusing, flow field phenomena, and dynamic cell-sorting demonstrate the advantages of the chip design. Numerical simulations in COMSOL are validated by the experimental data and used to investigate the effects of channel geometry and fluid properties on the flow field.

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Lu, Li, Rebecca M. Irwin, Jeffrey W. Schertzer e Paul R. Chiarot. "Particulate and Emulsion Sorting Using Microfluidics". In ASME 2014 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 2014. http://dx.doi.org/10.1115/imece2014-38298.

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We report on a microfluidic device capable of sorting nanoscale particulates and water-in-oil emulsions at high-throughput. The device is passive, relying solely on hydrodynamic forces and the emulsion mass to achieve separation. We use the microfluidic device to deliver surfactants and lipids to the emulsion surface. This is achieved by immersing the emulsions in a fluid stream with a high concentration of the nano-particulates. The particulates assemble on the surface of the emulsions as they are transported along the stream. The emulsions are then transferred (i.e. separated) into a second fluid stream that is devoid of surrounding material. The performance of the device is evaluated for a range of flow rates, nano-particulate concentrations, and emulsion sizes. We report separation efficiencies that exceed current technologies over a wide range of flow rates. The microfluidic device can be used to produce delivery vehicles for pharmaceuticals and models for membrane biology studies.

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Rapporti di organizzazioni sul tema "Microfluidic sorting"

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Wahl, Geoffrey M. A Novel Strategy for Isolation, Molecular and Functional Characterization of Embryonic Mammary Stem Cells Using Molecular Genetics and Microfluidic Sorting. Fort Belvoir, VA: Defense Technical Information Center, giugno 2008. http://dx.doi.org/10.21236/ada488861.

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