Letteratura scientifica selezionata sul tema "Marsupials Molecular genetics"

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Articoli di riviste sul tema "Marsupials Molecular genetics"

1

McKenzie, LM, e DW Cooper. "Low MHC class II variability in a marsupial". Reproduction, Fertility and Development 6, n. 6 (1994): 721. http://dx.doi.org/10.1071/rd9940721.

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Abstract (sommario):
The major histocompatibility complex (MHC) loci have been shown to be highly polymorphic in most eutherian ('placental') species studied. Several hypotheses have been advanced for the maintenance of this exceptional level of genetic variation, one of which suggests that it is necessary for successful eutherian reproduction. Marsupials (metatherians) and eutherians are the only two groups of viviparous mammals, but their modes of reproduction are quite distinct. Although marsupials have placentae, they are generally shorter lived and less invasive than in eutherians. Other investigations have shown that genetic variation at marsupial MHC class I loci is probably high. Weak or non-existent mixed lymphocyte culture responses previously reported in several marsupial species have suggested a lack of class II variation. Data have therefore been collected on the level of restriction fragment length polymorphism at MHC class II beta-chain encoding loci of a marsupial, Macropus eugenii (the tammar wallaby). This level is shown to be low, between the level of MHC variation found in cheetahs and a population of lions with a restricted genetic base. Attention is drawn to the need to collect more data on the level of class II variability in both eutherians and marsupials, and to the potential of marsupials for understanding the relation, if any, between mode of reproduction and MHC variability.
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2

Renfree, Marilyn B. "Society for Reproductive Biology Founders' Lecture 2006 Life in the pouch: womb with a view". Reproduction, Fertility and Development 18, n. 7 (2006): 721. http://dx.doi.org/10.1071/rd06072.

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Marsupials give birth to an undeveloped altricial young after a relatively short gestation period, but have a long and sophisticated lactation with the young usually developing in a pouch. Their viviparous mode of reproduction trades placentation for lactation, exchanging the umbilical cord for the teat. The special adaptations that marsupials have developed provide us with unique insights into the evolution of all mammalian reproduction. Marsupials hold many mammalian reproductive ‘records’, for example they have the shortest known gestation but the longest embryonic diapause, the smallest neonate but the longest sperm. They have contributed to our knowledge of many mammalian reproductive events including embryonic diapause and development, birth behaviour, sex determination, sexual differentiation, lactation and seasonal breeding. Because marsupials have been genetically isolated from eutherian mammals for over 125 million years, sequencing of the genome of two marsupial species has made comparative genomic biology an exciting and important new area of investigation. This review will show how the study of marsupials has widened our understanding of mammalian reproduction and development, highlighting some mechanisms that are so fundamental that they are shared by all today’s marsupial and eutherian mammals.
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3

Cowan, PE. "Changes in milk composition during lactation in the common brushtail possum, Trichosurus vulpecula (Marsupialia: Phalangeridae)". Reproduction, Fertility and Development 1, n. 4 (1989): 325. http://dx.doi.org/10.1071/rd9890325.

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The milk constituents of Trichosurus vulpecula, a folivorous marsupial, showed marked quantitative and qualitative changes during the course of lactation. The milk produced in the early stages of lactation was dilute, with about 9-13% (w/w) solids during the first 3 weeks, comprising mostly carbohydrate and protein (35-40%). At 20 weeks, about three-quarters of the way through lactation, the milk was much more concentrated, about 28% solids, with lipid the predominant fraction (30-35%), after a marked decline in carbohydrate content (20-25%). Concentrations of the electrolytes sodium and potassium also underwent marked changes. The changes in milk composition of T. vulpecula during the first three-quarters of lactation were similar to those described for a range of herbivorous, insectivorous and carnivorous marsupials. In the last quarter of lactation, however, brushtail possum milk maintained a relatively stable composition, with higher levels of carbohydrate and lower levels of lipid than for other marsupials. There appears to be a uniform pattern of changes in milk composition throughout the Marsupialia over most of lactation, with family differences evident only in the latter stages.
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4

Moore, HD. "Gamete biology of the new world marsupial, the grey short-tailed opossum, Monodelphis domestica". Reproduction, Fertility and Development 8, n. 4 (1996): 605. http://dx.doi.org/10.1071/rd9960605.

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Abstract (sommario):
Mammalian gametes undergo a series of functional and developmental changes that culminate in fertilization. In order to appreciate the necessity for such complex processes as sperm maturation, capacitation and the intimate sperm-egg interactions leading to gamete fusion, it is important to understand how gametes may have evolved. In this respect, marsupials are particularly relevant since they exhibit features reminiscent of both non-mammalian vertebrates and eutherian mammals. The grey short-tailed opossum, Monodelphis domestica, is a New World marsupial from Brazil. It breeds well under laboratory conditions and is an excellent animal model to investigate marsupial gamete biology. As in other American marsupials, the spermatozoa of the opossum form pairs in the epididymis. Here, a number of studies carried out in this laboratory, related to sperm maturation, capacitation and fertilization in M. domestica, are reviewed and the gamete biology in this species is compared with what is known in other marsupials and eutherian mammals.
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5

Price, Gilbert J., Kyle J. Ferguson, Gregory E. Webb, Yue-xing Feng, Pennilyn Higgins, Ai Duc Nguyen, Jian-xin Zhao, Renaud Joannes-Boyau e Julien Louys. "Seasonal migration of marsupial megafauna in Pleistocene Sahul (Australia–New Guinea)". Proceedings of the Royal Society B: Biological Sciences 284, n. 1863 (27 settembre 2017): 20170785. http://dx.doi.org/10.1098/rspb.2017.0785.

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Abstract (sommario):
Seasonal two-way migration is an ecological phenomenon observed in a wide range of large-bodied placental mammals, but is conspicuously absent in all modern marsupials. Most extant marsupials are typically smaller in body size in comparison to their migratory placental cousins, possibly limiting their potential to undertake long-distance seasonal migrations. But what about earlier, now-extinct giant marsupial megafauna? Here we present new geochemical analyses which show that the largest of the extinct marsupial herbivores, the enormous wombat-like Diprotodon optatum , undertook seasonal, two-way latitudinal migration in eastern Sahul (Pleistocene Australia–New Guinea). Our data infer that this giant marsupial had the potential to perform round-trip journeys of as much as 200 km annually, which is reminiscent of modern East African mammal migrations. These findings provide, to our knowledge, the first evidence for repetitive seasonal migration in any metatherian (including marsupials), living or extinct, and point to an ecological phenomenon absent from the continent since the Late Pleistocene.
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6

Renfree, M. B., S. R. Frankenberg e C. Freyer. "054. TROPHOBLAST, PLACENTA AND EARLY EMBRYO: HOW THE MARSUPIAL DEVELOPS". Reproduction, Fertility and Development 22, n. 9 (2010): 15. http://dx.doi.org/10.1071/srb10abs054.

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Abstract (sommario):
In marsupials, the blastocyst forms as a single cell layer of cells. The marsupial blastocyst has no inner cell mass, so the 80–100 cell tammar embryo remains in diapause as a unilaminar blastocyst. All marsupials have a unilaminar stage, but what is unusual is that in the tammar the total cessation of cell division and cell metabolism lasts for 11 months each year. Marsupials are placental mammals. The yolk sac forms the definitive placenta up to birth. Only very few marsupials, such as the bandicoot, have a chorio-allantoic placenta, which supplements the placental functions of the yolk sac. However, the understanding how the unilaminar layer of trophoblast cells of the diapausing blastocyst become specified into placental and embryonic tissues has been an ongoing puzzle. To identify genes that do become differentially expressed in tammar development, we targeted the stage of the earliest appearance of the embryonic disc, at which the remainder of the blastocyst is then defined as trophoblast, as well as early cleavage stages. Intriguingly, we found no evidence for early differential expression of the canonical pluripotency genes POU5F1, SOX2 and NANOG, or of CDX2. By contrast, we found overt differential expression of GATA3, the closely related gene GATA2, and FGF4. This expression profile suggests that in the tammar, mechanisms regulating trophoblast- and pluriblast-specific expression of POU5F1, SOX2, NANOG and CDX2 are temporally secondary to those regulating GATA2 & -3 and FGF4 expression. Together, our results may signify the evolution of alternative mechanisms of early lineage specification in marsupials, or alternatively reveal a general hierarchy of signalling mechanisms that are masked in the relatively rapid and ‘compressed’ development of mice. The results of our ongoing study have important implications for understanding not only marsupial stem cells but the early development of all therian mammals.
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Robinson, ES, MB Renfree, RV Short e JL VandeBerg. "Mammary glands in male marsupials. 2. Development of teat primordia in Didelphis virginiana and Monodelphis domestica". Reproduction, Fertility and Development 3, n. 3 (1991): 295. http://dx.doi.org/10.1071/rd9910295.

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Abstract (sommario):
Young and adults of both sexes of two didelphid marsupials, Didelphis virginiana and Monodelphis domestica, were examined externally for evidence of mammary gland development. Female young possessed teat numbers typical of adult females (13-15 in D. virginiana; 11-13 in M. domestica). Male young showed variable teat numbers which were always low compared with females, with the majority possessing 2-4 in anterior positions. Teats were also present in adult males of both species, in similar numbers and locations to those of young males. There are no previous reports of the presence of teats in any adult male marsupials. No mammary primordia in males have been recorded at any stage of development in the most thoroughly studied Australian marsupials. Our findings strengthen the view that there is a dichotomy between the two marsupial lineages in the regulation of male mammary gland expression.
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8

Deakin, Janine E., e Sally Potter. "Marsupial chromosomics: bridging the gap between genomes and chromosomes". Reproduction, Fertility and Development 31, n. 7 (2019): 1189. http://dx.doi.org/10.1071/rd18201.

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Abstract (sommario):
Marsupials have unique features that make them particularly interesting to study, and sequencing of marsupial genomes is helping to understand their evolution. A decade ago, it was a huge feat to sequence the first marsupial genome. Now, the advances in sequencing technology have made the sequencing of many more marsupial genomes possible. However, the DNA sequence is only one component of the structures it is packaged into: chromosomes. Knowing the arrangement of the DNA sequence on each chromosome is essential for a genome assembly to be used to its full potential. The importance of combining sequence information with cytogenetics has previously been demonstrated for rapidly evolving regions of the genome, such as the sex chromosomes, as well as for reconstructing the ancestral marsupial karyotype and understanding the chromosome rearrangements involved in the Tasmanian devil facial tumour disease. Despite the recent advances in sequencing technology assisting in genome assembly, physical anchoring of the sequence to chromosomes is required to achieve a chromosome-level assembly. Once chromosome-level assemblies are achieved for more marsupials, we will be able to investigate changes in the packaging and interactions between chromosomes to gain an understanding of the role genome architecture has played during marsupial evolution.
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9

Rodger, JC. "Prefertilization gamete maturation events in marsupials". Reproduction, Fertility and Development 6, n. 4 (1994): 473. http://dx.doi.org/10.1071/rd9940473.

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Abstract (sommario):
Despite many fundamental similarities between the gametes of marsupials and placental mammals, the regulation and timing of prefertilization gamete maturation are quite different. The marsupial acrosome is remarkably stable and an acrosome reaction (AR) is not induced by reagent effective for the sperm of placental mammals. The ultrastructure of the marsupial sperm AR is essentially similar to that of placental mammals, however, whether an equatorial segment (ES) persists to serve as the site of sperm-egg membrane fusion is unclear. Diacylglycerol induction of the AR suggests that the sperm of Australian species lack an ES, yet an ES-like region appears to be involved in fertilization in the opossum Monodelphis. The marsupial oocyte, unlike those of placentals, continues to grow throughout follicular life and major cytoplasmic maturation events occur late in oocyte development. Cortical granules only become evident shortly before ovulation and mature dark granules may only appear after ovulation. Further, the zona pellucida (ZP) changes in character and function during the peri-ovulatory period. In vitro fertilization has been achieved for an opossum but not for any Australian marsupial, owing to failure of sperm-ZP binding. Requirement for a sperm maturation process is likely, but capacitation treatments used for placental sperm in vitro have been ineffective. Since it is now feasible to experimentally manipulate marsupial gametes in vitro major advances in our understanding of their function can be expected.
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10

Frankenberg, S., A. J. Pask e M. B. Renfree. "259. Pluripotency genes in a marsupial, the tammar wallaby". Reproduction, Fertility and Development 20, n. 9 (2008): 59. http://dx.doi.org/10.1071/srb08abs259.

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Abstract (sommario):
Markers of pluripotency and early differentiation in the early embryo have been extensively characterised in eutherian species, most notably the mouse. By comparison, mechanisms controlling pluripotency and early lineage specification have received surprisingly little attention in marsupials, which represent the second major infraclass of mammals. Early marsupial embryogenesis exhibits overt morphological differences to that of eutherians, however the underlying developmental mechanisms may be conserved. In order to characterise early marsupial development at the molecular level, we have identified, cloned and analysed expression of orthologueues of several eutherian genes encoding transcription factors and signalling molecules involved in regulating pluripotency and early lineage specification. These genes include POU5F1 (OCT4), SOX2, NANOG, FGF4, FGFR2, CDX2, EOMES, TEAD4, GATA6 and KITL and are all expressed at early stages of development in the tammar. In addition, we have identified and cloned tammar POU2, which has orthologueues in non-mammalian vertebrates. POU2 is a paralogue of POU5F1 – a master regulator of pluripotency in eutherians. Genomic analysis indicates that POU5F1 arose via gene duplication of POU2 before the monotreme-therian divergence. Both genes have persisted in marsupials and monotremes, while POU2 was lost early during eutherian evolution. Similar expression profiles of tammar POU5F1 and POU2 in early embryos and gonadal tissues suggest possible overlapping roles in the maintenance of pluripotency.
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Tesi sul tema "Marsupials Molecular genetics"

1

Firestone, Karen Beth School of Biological Science UNSW. "The Application of Molecular Genetics to the Conservation Management of Quolls, Dasyurus Species (Dasyuridae:Marsupialia)". Awarded by:University of New South Wales. School of Biological Science, 1999. http://handle.unsw.edu.au/1959.4/17491.

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Abstract (sommario):
The quolls are among the largest of the remaining carnivorous marsupials in the Australasian region, and thus occupy an important ecological niche as top predators and scavengers. All quolls are currently in decline and threatened to some degree yet the application of molecular information to the conservation and management of quolls has been unexplored until now. In this thesis I use two independent and highly variable genetic marker systems, the mitochondrial DNA (mtDNA) control region and nuclear microsatellites, to explore various aspects of conservation genetics relevant to the management of quolls. These aspects include an examination of the phylogenetic or evolutionary relationships among all six species of quolls, an examination of the genetic diversity within populations and the degree of differentiation between populations of the four Australian species of quolls, and the definition of units for conservation within these species. The development of suitable nuclear markers was a vital first step in defining levels of genetic variability and differentiation within and between the different populations and species. These markers proved to be highly variable and provided a wealth of information of relevance to the conservation of these species, and will be extremely useful in further studies. The use of the mtDNA control region for phylogenetic analyses was a novel approach to examining this question in quolls and also proved to be highly informative. Results from these phylogenetic analyses highlight the necessity of 1) examining more than one exemplar of each species, as well as 2) finally bringing some consensus to the question of the evolutionary relationships among quolls. Results show that northern quolls form the earliest split from all other quolls and that western quolls are closely related to the two New Guinean species. Furthermore, there is evidence for distinct lineages within species, corresponding to geographically separate or isolated populations. Levels of genetic variability within populations were examined using the microsatellites developed previously. Genetic variation was significantly higher in western quolls than in any other species. This was surprising given the long term and widespread decline of this species. There were also significant differences between populations within species in the level of genetic variability. Low levels of variability were usually found in small or captive bred populations or populations in severe decline. Genetic differentiation between populations was also explored using microsatellites. Significant differentiation in allele frequency distributions was found between most pairwise population comparisons, indicating that each of these populations forms a separate management unit (MU) for conservation purposes. One notable exception was found among populations of tiger quolls from a highly localized area in the Barrington Tops region of New South Wales. Using microsatellites, these populations were not significantly subdivided and thus appeared to be one MU. Using mtDNA, however, these populations were significantly subdivided and thus should be considered separate MUs. Differences in the way these two genetic markers are inherited (mtDNA is maternally inherited, microsatellites are biparentally inherited) provides a clue as to the social structure and organization of these cryptic nocturnal species. Consequently, the use of different genetic marker systems shows that there is sex-biased migration within this species. Finally, the degree of genetic differentiation observed within tiger quolls does not conform to the currently recognized subspecific categories within this species. The major genetic split occurs between the Tasmanian and mainland populations of tiger quolls, not between Dasyurus maculatus maculatus and D. m. gracilis. Thus, the Tasmanian and mainland populations form two distinct evolutionarily significant units (ESUs) for conservation purposes, and I propose that the Tasmanian populations should be elevated to the subspecific status to account for this.
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Fazenda, Inês Isabel Paulo. "Molecular and morphological characterization of the genus globocephaloides from macropodid marsupials in Australia". Bachelor's thesis, Universidade Técnica de Lisboa. Faculdade de Medicina Veterinária, 2009. http://hdl.handle.net/10400.5/1302.

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Dissertação de Mestrado Integrado em Medicina Veterinária
The genus Globocephaloides (Nematoda: Trichostrongyloidea) is a pathogenic group of parasitic nematodes present in the duodenum of kangaroos and wallabies (Marsupialia: Macropodidae) in Australia. Globocephaloides species (G. trifidospicularis, G. macropodis and G. affinis) have been poorly studied and thus, there are significant controversies regarding their systematics and population structures. In the present study, single-strand conformation polymorphism (SSCP) and targeted sequencing of the internal transcribed spacers (ITS) of nuclear ribosomal DNA, were used to assess the genetic variation within and among Globocephaloides populations and individuals, from different host species and geographical origins. No or minor (0.2%) variation was detected among individuals of G. trifidospicularis and G. affinis. However, within G. macropodis populations there was a consistent heterogeneity in the ITS sequences (5.2 - 7.1%) between worms derived from two different host species (Macropus agilis and M. dorsalis). Under light microscopy, these two G. macropodis genotypes differed by the length, arrangement and tip of the spicules, and by pattern of the bursal rays. Thus, they were considered to represent sibling species. The molecular and morphological evidence culminated with the erection of a new species, namely G. wallabiae, and provided further insights into the host affiliation and geographical ranges of Globocephaloides spp.: G. wallabiae occurs mainly in M. dorsalis (north/east), G. macropodis in M. agilis and Petrogale persephone (north), G. affinis in M. dorsalis (north-east), and G. trifidospicularis in various Macropus species (south). Moreover, in the present study, the phylogenetic analyses between the genus Globocephaloides and other trichostrongyloid genera, using divergent domains of the 28S rRNA genes, gave evidence for the exclusion of the genus Globocephaloides from the Herpetostrongylidae, with the suggestion of a new family, namely „Globocephaloididae‟, within the Heligmosomoidea.
RESUMO - CARACTERIZAÇÃO MOLECULAR E MORFOLÓGICA DO GÉNERO GLOBOCEPHALOIDES EM MARSUPIAIS MACROPODÍDEOS NA AUSTRÁLIA - O género Globocephaloides (Nematoda: Trichostrongyloidea) é um importante grupo de nemátodes patogénicos presente no duodeno de cangurus e wallabies (Marsupialia: Macropodidae) na Austrália. O estudo das espécies de Globocephaloides (G. trifidospicularis, G. macropodis e G. affinis) tem sido limitado, e por isso, existem numerosas controvérsias em relação à sua sistemática e às suas estruturas populacionais. No presente estudo, as técnicas de análise de ácidos nucleicos, „single-strand conformation polymorphism‟ (SSCP) e sequenciação-alvo da região ITS („internal transcribed spacers‟) do DNA ribossomal foram usadas com o objectivo de analisar a variação genética entre indivíduos e populações de Globocephaloides provenientes de diferentes hospedeiros e áreas geográficas. Em ambas as espécies G. trifidospicularis e G. affinis pouca ou nenhuma variação foi encontrada (0.2%). Contudo, nas populações de G. macropodis foi detectada uma consistente heterogeneidade nas sequências ITS (5.2 - 7.1%) entre espécimes provenientes de dois hospedeiros distintos (Macropus agilis e M. dorsalis). Recorrendo à microscopia óptica verificou-se que os dois genótipos de G. macropodis diferiam no comprimento, conformação e ponta das espículas, assim como, no padrão dos raios da bolsa copuladora. Como tal, foi considerado que os dois genótipos representavam duas espécies congéneres. Os resultados da biologia molecular e do estudo morfológico determinaram o reconhecimento de uma nova espécie denominada G. wallabiae e, além disso, forneceram dados sobre a especificidade de hospedeiros e distribuição geográfica do género Globocephaloides. G. wallabiae ocorre principalmente no hospedeiro M. dorsalis (norte/este), G. macropodis no M. agilis e Petrogale persephone (norte), G. affinis no M. dorsalis (nordeste) e G. trifidospicularis em várias espécies do género Macropus (sul). Adicionalmente, a análise das relações filogenéticas entre o género Globocephaloides e outros tricostrongilídeos, empregando os domínios do gene 28S rRNA, deu provas para a exclusão do género Globocephaloides da família Herpetostrongylidae, com a sugestão de uma nova família, denominada „Globocephalidae‟, inserida na superfamília Heligmosomoidea.
Financial support provided to the supervisors for the present study was from bodies including the Australian Research Council (ARC) [to Robin B. Gasser] and Australian Biological Resources Study (ABRS) [to Ian Beveridge]
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Rosa, Pamela Laiz Paré da. "Evolução dos genes da rede OXT - AVP - PRL: Aspectos moleculares, fisiológicos e comportamentais em mamíferos". reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2018. http://hdl.handle.net/10183/180703.

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Abstract (sommario):
A busca pelo repertório genético por trás de características comportamentais e reprodutivas de espécies de primatas tem desafiado nosso grupo de pesquisa. A premissa principal nesse tipo de estudo baseia-se na hipótese de que um traço fenotípico (seja ele fisiológico, comportamental, etc) compartilhado por um grupo taxonômico inteiro deve ser determinado por um repertório genético comum a esses táxons. Considerando a complexidade de muitas dessas características, temos ampliado nossos estudos para vários genes da rede OXT – AVP – PRL, usando abordagens in silico, in vitro, e in vivo. Na presente Tese, o conjunto gênico de estudo foi selecionado por uma metodologia baseada na ontologia biológica, com critério de seleção para características como comportamento materno, amamentação e aspectos reprodutivos, tais como comportamento de acasalamento e de corte. Essa seleção resultou em 12 genes candidatos para o estudo: AVP, AVPR1A, AVPR1B, ESR1, FOS, HCRT, OXT, OXTR, PRL, PRLH, PRLR e TRH. Exploramos aqui esse conjunto gênico da rede OXT – AVP – PRL através da mineração de dados, buscando por seus ortólogos nas várias espécies de primatas e de outros mamíferos, bem como através de novos dados de sequências da região codificadora dos genes PRLR e PRLH, em um conjunto de espécies de primatas do Novo Mundo (NWM, conforme sigla em inglês). Adicionalmente, sequências das regiões codificadoras de PRLR foram também obtidos em espécies de marsupiais. Com o objetivo de elucidar os padrões evolutivos dos genes de interesse, utilizamos análises como os testes NsSites e Branch Sites do pacote PAML, assim como vários testes populacionais clássicos, para diferentes conjuntos amostrais. Além disso, foi feita a predição da estrutura secundária das proteínas-alvo, utilizando metodologia específica do programa PSIPRED, bem como o PONDER-FIT, para a caracterização de aminoácidos intrinsecamente desordenados. Nossos resultados das análises in silico sugerem que os genes da família de receptores da vasopressina (AVPR1A, AVPR1B, e AVPR2) apresentam um padrão compatível com seleção positiva em mamíferos placentários. Alguns dos sítios com sinal de seleção apresentam motivos lineares (SLiMS) preditos no receptor AVPR2, que podem ter facilitado a emergência de novidades adaptativas, conforme foi sugerido para a espécie do rato-canguru Dipodomys ordii, que habita regiões áridas. As análises dos dados originais da região codificadora do gene PRLR em 17 espécies de NWM revelaram vários sítios presentes na forma longa do receptor com alta probabilidade de estarem sob seleção positiva, sendo que alguns deles (posições 507, 532 e 572) estão associados com o parto gemelar, uma característica das espécies de Callitrichidae. Adicionalmente verificamos no ramo dos Siimiformes um motivo linear de interação que reconhece domínios SH3 (Src Homology 3). Os domínios SH3 e os seus locais de ligação foram descritos para centenas de proteínas; eles fornecem à célula um meio particularmente conveniente e adaptável de interação específica proteína-proteína, que pode ser de importância funcional. Esse trabalho como um todo contribuiu para o conhecimento do repertório genético relacionado à complexa rede de mecanismos neuroendócrinos associados à emergência de traços adaptativos, tanto fisiológicos quanto comportamentais em diferentes clados de mamíferos.
The search for the genetic repertoire behind behavioral and reproductive features of Primate species has challenged our research group. The principal premise in this kind of study is based on the hypothesis that a phenotypic trait (either physiological, behavioral, etc.) shared by an entire taxonomic group should be determined by a genetic repertoire common to these taxa. Considering the complexity of many of these features, we have expanded our studies for several genes of the OXT - AVP - PRL network, using in silico, in vitro, and in vivo approaches. In the present Thesis, the set of genes for the study was selected by a methodology based on biological ontology, with features like maternal behavior, breastfeeding, and reproductive aspects, such as mating and courtship behavior. This selection resulted in 12 candidate genes for the study: AVP, AVPR1A, AVPR1B, ESR1, FOS, HCRT, OXT, OXTR, PRL, PRLH, PRLR, and TRH. We explored here this gene set of the OXT – AVP – PRL network through data mining, searching for their orthologues in many Primate species and other mammals, as well as through new sequence data from the PRLR and PRLH coding region in a set of New World Monkey (NWM) species. Additionally, sequences from the PRLR coding regions were also obtained in marsupial species. To elucidate evolutionary patterns of the genes of interest, we used the NsSites and Branch Sites tests from PAML package, as well as several classic population tests, for different sample sets. In addition, we predicted the secondary structure of target proteins, using a specific methodology of the PSIPRED program, as well as PONDER-FIT for prediction of intrinsically disordered amino acids. Our in silico results suggest that the genes of the vasopressin receptor family (AVPR1A, AVPR1B, and AVPR2) present a pattern compatible with positive selection in placental mammals. Some of the sites with selection signals have linear motifs (SLiMS) predicted in the AVPR2 receptor, which may have facilitated the emergence of adaptive novelties, as was suggested for the kangaroo rat Dipodomys ordii, which inhabits arid regions. Analyses of the original PRLR coding region data on 17 NWM species revealed several sites present in the long form of the receptor with a high probability of being under positive selection, some of them (positions 507, 532 and 572) being associated with twin births, a characteristic of Callitrichidae species. Additionally, we verified in the Siimiformes branch a linear interaction motif that recognizes SH3 domains (Src Homology 3). The SH3 domains and their ligands were described for hundreds of proteins; they provide a particularly convenient and adaptable medium of specific protein-protein interaction to the cell, which can be of functional importance. This work as a whole contributed to the knowledge of the genetic repertoire connected to the complex network of neuroendocrine mechanisms associated to the emergence of physiological and behavioral adaptive traits in different mammalian clades.
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4

PAVAN, Silvia Eliza D´Oliveira. "Diversidade de espécies no complexo Monodelphis brevicaudata (Didelphimorphia:Didelphidae), inferida por dados moleculares e morfológicos". Universidade Federal do Pará, 2009. http://repositorio.ufpa.br/jspui/handle/2011/3535.

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O complexo de espécies M. brevicaudata possui distribuição reconhecida para o Norte da América do sul e compreende três espécies descritas ‐ M. brevicaudata, M. glirina, e M. palliolata ‐ e duas não descritas, reconhecidas em estudos prévios. A delimitação de espécies baseada somente em caracteres morfológicos é complicada, de forma que diversos táxons nominais já foram associados ao grupo e diversos arranjos taxonômicos foram propostos. Os poucos estudos baseados em dados moleculares que incluíram espécimes do complexo brevicaudata revelaram altas taxas de divergência genética. Este trabalho buscou elucidar a sistemática do complexo de espécies M. brevicaudata através do estudo dos padrões de variação morfológica e genética. Para tal, desenvolvemos análises filogenéticas baseadas em dois genes mitocondriais: citocromo b e 16 S rDNA. Adicionalmente, estudamos a morfologia externa e craniana dos espécimes, investigando a existência de congruência entre a variação genética e morfológica. As análises morfológicas foram, em geral, congruentes com as moleculares, as quais indicaram os mesmos clados em todas as análises filogenéticas. Foram formalmente reconhecidas nove espécies para o complexo. Monodelphis brevicaudata, M. palliolata e M. glirina são consideradas espécies válidas; M. touan é revalidado da sinonímia de M. brevicaudata e duas espécies novas são descritas e nomeadas; a espécie M. domestica provou ser intimamente relacionada a espécimes do grupo brevicaudata, sendo aqui considerada como integrante do referido grupo; duas espécies reconhecidas como distintas permanecem sem uma descrição formal; M. maraxina é sinonimizada com M. glirina. Foi observado dimorfismo sexual para as espécies estudadas, sendo que para as duas espécies estatisticamente testadas (teste T de student), M. glirina e M. sp. nov. “Trombetas”, os machos apresentaram crânios significativamente maiores que as fêmeas. Rios de grande porte parecem ter participado na diferenciação genética e estruturação filogeográfica das espécies. O padrão filogeográfico encontrado sugere ao menos dois centros de diversificação para o grupo, um no escudo das Guianas, envolvendo as espécies ao norte do rio Amazonas, e outro no escudo brasileiro, envolvendo M. glirina e M. domestica.
Short‐tailed opossums of the Monodelphis revicaudata complex inhabit northern South America, and comprise three described species ‐ M. brevicaudata, M. glirina, and M. palliolata ‐ and two undescribed forms already recognized in prior studies. Species delimitation based solely on morphological features is difficult, and because of that many nominal taxa have been associated with this species complex, and several taxonomic arrangements have been proposed. Previous molecular phylogenetic studies using specimens of this species complex revealed substantial genetic divergence rates. The present study aims to elucidate the systematics of the M. brevicaudata species complex through the analyses of molecular and morphological characters. We performed phylogenetic analyses on two mitochondrial genes (cyt b and 16S), studied the external and cranial morphology, and investigated whether observed genetic variation is congruent with morphological differences. Our morphological results were generally concordant with the molecular results. We recognize nine species in the species complex. M. brevicaudata, M. palliolata, and M. glirina are considered valid species; M. touan is re‐established from the synonymy of M. brevicaudata and two new species are described and named; the species M. domestica proved to be closely related to specimens of the M. brevicaudata complex, and thus are considered as part of that group; we also recognized two new species without formallly naming them; M. maraxina is considered a synonym of M. glirina. Sexual dimorphism is observed in the species, and in two species males showed skulls significantly larger than females. Major rivers seem to have played an important role in generating genetic differentiation and phylogeographical structure of the species. The phylogeographical pattern suggests at least two diversification centers for the group, one in the Guiana shield, comprising species ranging north of the Amazon river, and another in the Brazilian shield, comprising M. glirina and M. domestica.
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5

Firestone, Karen B. "The application of molecular genetics to the conservation management of quolls, Dasyurus species (Dasyuridae : Marsupialia) /". 1999. http://www.library.unsw.edu.au/~thesis/adt-NUN/public/adt-NUN20010105.095232/index.html.

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6

Sankovic, Natasha. "Molecular characterisation of a marsupial Y chromosome". Phd thesis, 2005. http://hdl.handle.net/1885/150771.

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7

Malekian, Mansoureh. "Molecular systematics and conservation genetics of gliding petaurids (Marsupialia: petauridae)". 2007. http://hdl.handle.net/2440/41539.

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The gliding petaurids are small sized arboreal and nocturnal marsupials restricted to Australia and the New Guinean region. They have suffered range contractions since European settlement, and most of the species are of conservation concern, either nationally or at a state level. This study applied molecular approaches to investigate several questions involving Petaurus species which may provide valuable insights for their conservation and management of species. The objectives of this study included an examination of phylogenetic and evolutionary relationships among Petaurus species, an assessment of phylogeographic structure within P. breviceps and an investigation of genetic diversity, social structure and mating system of P. breviceps in fragmented habitats. A broad molecular systematics study of the genus Petaurus was first undertaken. Two mitochondrial genes (ND2 and ND4) and a nuclear gene marker (ω-globin) were screened for sequence variation in samples obtained from across the distribution of petaurid species, including Australia, New Guinea and its surrounding islands. Phylogenetic analyses confirmed the monophyly of the genus Petaurus and revealed that, with the exception of P. gracilis, the currently recognised species were associated with divergent mtDNA clades. It also revealed considerable mtDNA diversity within the widely distributed species P. breviceps. The existence of at least seven distinct and divergent mtDNA lineages within P. breviceps was supported, with two lineages located in Australia and at least five lineages in New Guinea. However, the distribution of these evolutionary lineages did not correspond with current morphological subspecies boundaries. Analyses of ω-globin sequence provided support for a number of these distinct populations, suggesting the possible presence of cryptic species within P. breviceps. Molecular analyses also suggested that squirrel gliders, P. norfolcensis, may occur in both South Australia and the Northern Territory, extending the current known range of the species. The presence of P. norfolcensis in SA was further verified by examining museum skins. Population structure and current pattern of gene flow within P. breviceps in Australia was examined further to elucidate phylogeographic structure within the species, and explore potential causes of geographic variation. Evidence for significant phylogeographic structuring across the range of the species in Australia was provided from population genetic (AMOVA) and phylogenetic analyses of both mitochondrial DNA and the ω-globin gene. In particular, there was evidence for the existence of two divergent clades that were distributed over distinct geographical regions. Divergence dates calculated for the two major mtDNA clades suggested that environment and climate changes which occurred during the Pliocene may have facilitated this diversification. Habitat fragmentation is generally considered to be a major factor threatening the viability of forest dependent species such as gliders. Effects of habitat fragmentation were therefore investigated in P. breviceps in the highly disturbed landscape of southeastern South Australia. Genetic mating system and social structure of the species in these fragmented habitats was explored in 13 populations, using nine polymorphic microsatellite loci. Social groups consisted of two to seven gliders, and these were often close relatives, including parents with their offspring. Parentage analyses provided some evidence for a polygamous mating system, with a number of males found to have fathered offspring from multiple female partners. Some direct evidence of inbreeding was also found within a small isolated patch. Genetic diversity within P. breviceps populations was moderate compared to the range reported in other marsupial species. Population structure analyses indicated that gene flow between some patches was restricted. Small patches surrounded by a matrix of pine were more likely to show inbreeding and potentially suffer from inbreeding depression, although further data are required to verify this result. Overall, results suggest that, although the species is still present in these small and isolated patches, it may face threats from a lack of dispersal and inbreeding. Maintaining the size of patches and establishing corridors between isolated populations needs to be considered in conservation and management of species in these fragmented habitats.
http://library.adelaide.edu.au/cgi-bin/Pwebrecon.cgi?BBID=1295224
Thesis (Ph.D.) -- School of Earth and Enviromental Sciences, 2007
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8

Malekian, Mansoureh. "Molecular systematics and conservation genetics of gliding petaurids (Marsupialia: petauridae)". Thesis, 2007. http://hdl.handle.net/2440/41539.

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Abstract (sommario):
The gliding petaurids are small sized arboreal and nocturnal marsupials restricted to Australia and the New Guinean region. They have suffered range contractions since European settlement, and most of the species are of conservation concern, either nationally or at a state level. This study applied molecular approaches to investigate several questions involving Petaurus species which may provide valuable insights for their conservation and management of species. The objectives of this study included an examination of phylogenetic and evolutionary relationships among Petaurus species, an assessment of phylogeographic structure within P. breviceps and an investigation of genetic diversity, social structure and mating system of P. breviceps in fragmented habitats. A broad molecular systematics study of the genus Petaurus was first undertaken. Two mitochondrial genes (ND2 and ND4) and a nuclear gene marker (ω-globin) were screened for sequence variation in samples obtained from across the distribution of petaurid species, including Australia, New Guinea and its surrounding islands. Phylogenetic analyses confirmed the monophyly of the genus Petaurus and revealed that, with the exception of P. gracilis, the currently recognised species were associated with divergent mtDNA clades. It also revealed considerable mtDNA diversity within the widely distributed species P. breviceps. The existence of at least seven distinct and divergent mtDNA lineages within P. breviceps was supported, with two lineages located in Australia and at least five lineages in New Guinea. However, the distribution of these evolutionary lineages did not correspond with current morphological subspecies boundaries. Analyses of ω-globin sequence provided support for a number of these distinct populations, suggesting the possible presence of cryptic species within P. breviceps. Molecular analyses also suggested that squirrel gliders, P. norfolcensis, may occur in both South Australia and the Northern Territory, extending the current known range of the species. The presence of P. norfolcensis in SA was further verified by examining museum skins. Population structure and current pattern of gene flow within P. breviceps in Australia was examined further to elucidate phylogeographic structure within the species, and explore potential causes of geographic variation. Evidence for significant phylogeographic structuring across the range of the species in Australia was provided from population genetic (AMOVA) and phylogenetic analyses of both mitochondrial DNA and the ω-globin gene. In particular, there was evidence for the existence of two divergent clades that were distributed over distinct geographical regions. Divergence dates calculated for the two major mtDNA clades suggested that environment and climate changes which occurred during the Pliocene may have facilitated this diversification. Habitat fragmentation is generally considered to be a major factor threatening the viability of forest dependent species such as gliders. Effects of habitat fragmentation were therefore investigated in P. breviceps in the highly disturbed landscape of southeastern South Australia. Genetic mating system and social structure of the species in these fragmented habitats was explored in 13 populations, using nine polymorphic microsatellite loci. Social groups consisted of two to seven gliders, and these were often close relatives, including parents with their offspring. Parentage analyses provided some evidence for a polygamous mating system, with a number of males found to have fathered offspring from multiple female partners. Some direct evidence of inbreeding was also found within a small isolated patch. Genetic diversity within P. breviceps populations was moderate compared to the range reported in other marsupial species. Population structure analyses indicated that gene flow between some patches was restricted. Small patches surrounded by a matrix of pine were more likely to show inbreeding and potentially suffer from inbreeding depression, although further data are required to verify this result. Overall, results suggest that, although the species is still present in these small and isolated patches, it may face threats from a lack of dispersal and inbreeding. Maintaining the size of patches and establishing corridors between isolated populations needs to be considered in conservation and management of species in these fragmented habitats.
Thesis (Ph.D.) -- School of Earth and Enviromental Sciences, 2007
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Libri sul tema "Marsupials Molecular genetics"

1

Coulson, Graeme, e Mark Eldridge, a cura di. Macropods. CSIRO Publishing, 2010. http://dx.doi.org/10.1071/9780643098183.

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This book covers the proceedings of a major 2006 symposium on macropods that brought together the many recent advances in the biology of this diverse group of marsupials, including research on some of the much neglected macropods such as the antilopine wallaroo, the swamp wallaby and tree-kangaroos. More than 80 authors have contributed 32 chapters, which are grouped into four themes: genetics, reproduction and development; morphology and physiology; ecology; and management. The book examines such topics as embryonic development, immune function, molar progression and mesial drift, locomotory energetics, non-shivering thermogenesis, mycophagy, habitat preferences, population dynamics, juvenile mortality in drought, harvesting, overabundant species, road-kills, fertility control, threatened species, cross-fostering, translocation and reintroduction. It also highlights the application of new techniques, from genomics to GIS. Macropods is an important reference for academics and students, researchers in molecular and ecological sciences, wildlife and park managers, and naturalists.
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Capitoli di libri sul tema "Marsupials Molecular genetics"

1

Pask, Andrew J., e Marilyn B. Renfree. "Molecular Regulation of Marsupial Reproduction and Development". In Marsupial Genetics and Genomics, 285–316. Dordrecht: Springer Netherlands, 2010. http://dx.doi.org/10.1007/978-90-481-9023-2_14.

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2

Hampikian, Gregory K., Desmond W. Cooper e Jennifer A. Marshall Graves. "Sex Determination in Marsupials and Monotremes". In Molecular Genetics of Sex Determination, 143–70. Elsevier, 1994. http://dx.doi.org/10.1016/b978-0-12-728960-1.50012-4.

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