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Articoli di riviste sul tema "LuxR"

Autore: Grafiati

Pubblicato: 4 giugno 2021

Ultima modifica: 11 marzo 2023

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1

Bazhenov, Sergey, Olga Melkina, Vadim Fomin, Ekaterina Scheglova, Pavel Krasnik, Svetlana Khrulnova, Gennadii Zavilgelsky, and Ilya Manukhov. "LitR directly upregulates autoinducer synthesis and luminescence in Aliivibrio logei." PeerJ 9 (September 21, 2021): e12030. http://dx.doi.org/10.7717/peerj.12030.

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LitR is a master-regulator of transcription in the ainS/R and luxS/PQ quorum sensing (QS) systems of bacteria from Vibrio and Aliivibrio genera. Here, we for the first time directly investigated the influence of LitR on gene expression in the luxI/R QS system of psychrophilic bacteria Aliivibrio logei. Investigated promoters were fused with Photorhabdus luminescens luxCDABE reporter genes cassette in a heterological system of Escherichia coli cells, litR A. logei was introduced into the cells under control of Plac promoter. LitR has been shown to upregulate genes of autoinducer synthase (luxI)

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Bazhenov, Sergey, Uliana Novoyatlova, Ekaterina Scheglova, Vadim Fomin, Svetlana Khrulnova, Olga Melkina, Vladimir Chistyakov, and Ilya Manukhov. "Influence of the luxR Regulatory Gene Dosage and Expression Level on the Sensitivity of the Whole-Cell Biosensor to Acyl-Homoserine Lactone." Biosensors 11, no. 6 (May 23, 2021): 166. http://dx.doi.org/10.3390/bios11060166.

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Aliivibrio fischeri LuxR and Aliivibrio logei LuxR1 and LuxR2 regulatory proteins are quorum sensing transcriptional (QS) activators, inducing promoters of luxICDABEG genes in the presence of an autoinducer (3-oxo-hexanoyl-l-homoserine lactone). In the Aliivibrio cells, luxR genes are regulated by HNS, CRP, LitR, etc. Here we investigated the role of the luxR expression level in LuxI/R QS system functionality and improved the whole-cell biosensor for autoinducer detection. Escherichia coli-based bacterial lux-biosensors were used, in which Photorhabdus luminescensluxCDABE genes were controlled

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3

Urbanowski, M. L., C. P. Lostroh, and E. P. Greenberg. "Reversible Acyl-Homoserine Lactone Binding to Purified Vibrio fischeri LuxR Protein." Journal of Bacteriology 186, no. 3 (February 1, 2004): 631–37. http://dx.doi.org/10.1128/jb.186.3.631-637.2004.

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ABSTRACT The Vibrio fischeri LuxR protein is the founding member of a family of acyl-homoserine lactone-responsive quorum-sensing transcription factors. Previous genetic evidence indicates that in the presence of its quorum-sensing signal, N-(3-oxohexanoyl) homoserine lactone (3OC6-HSL), LuxR binds to lux box DNA within the promoter region of the luxI gene and activates transcription of the luxICDABEG luminescence operon. We have purified LuxR from recombinant Escherichia coli. Purified LuxR binds specifically and with high affinity to DNA containing a lux box. This binding requires addition o

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4

Antunes, Luis Caetano M., Rosana B. R. Ferreira, C. Phoebe Lostroh, and E. Peter Greenberg. "A Mutational Analysis Defines Vibrio fischeri LuxR Binding Sites." Journal of Bacteriology 190, no. 13 (December 14, 2007): 4392–97. http://dx.doi.org/10.1128/jb.01443-07.

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ABSTRACT Vibrio fischeri quorum sensing involves the LuxI and LuxR proteins. The LuxI protein generates the quorum-sensing signal N-3-oxohexanoyl-l-homoserine lactone (3OC6-HSL), and LuxR is a signal-responsive transcriptional regulator which activates the luminescence (lux) genes and 17 other V. fischeri genes. For activation of the lux genes, LuxR binds to a 20-base-pair inverted repeat, the lux box, which is centered 42.5 base pairs upstream of the transcriptional start of the lux operon. Similar lux box-like elements have been identified in only a few of the LuxR-activated V. fischeri prom

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Swearingen, Matthew C., Anice Sabag-Daigle, and Brian M. M. Ahmer. "Are There Acyl-Homoserine Lactones within Mammalian Intestines?" Journal of Bacteriology 195, no. 2 (November 9, 2012): 173–79. http://dx.doi.org/10.1128/jb.01341-12.

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ABSTRACTManyProteobacteriaare capable of quorum sensing usingN-acyl-homoserine lactone (acyl-HSL) signaling molecules that are synthesized by LuxI or LuxM homologs and detected by transcription factors of the LuxR family. Most quorum-sensing species have at least one LuxR and one LuxI homolog. However, members of theEscherichia,Salmonella,Klebsiella, andEnterobactergenera possess only a single LuxR homolog, SdiA, and no acyl-HSL synthase. The most obvious hypothesis is that these organisms are eavesdropping on acyl-HSL production within the complex microbial communities of the mammalian intest

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6

Antunes, Luis Caetano M., Amy L. Schaefer, Rosana B. R. Ferreira, Nan Qin, Ann M. Stevens, Edward G. Ruby, and E. Peter Greenberg. "Transcriptome Analysis of the Vibrio fischeri LuxR-LuxI Regulon." Journal of Bacteriology 189, no. 22 (September 7, 2007): 8387–91. http://dx.doi.org/10.1128/jb.00736-07.

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ABSTRACT The Vibrio fischeri quorum-sensing signal N-3-oxohexanoyl-l-homoserine lactone (3OC6-HSL) activates expression of the seven-gene luminescence operon. We used microarrays to unveil 18 additional 3OC6-HSL-controlled genes, 3 of which had been identified by other means previously. We show most of these genes are regulated by the 3OC6-HSL-responsive transcriptional regulator LuxR directly. This demonstrates that V. fischeri quorum sensing regulates a substantial number of genes other than those involved in light production.

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7

von Bodman, Susanne B., Jessica K. Ball, Marie A. Faini, Carmen M. Herrera, Timothy D. Minogue, Mark L. Urbanowski, and Ann M. Stevens. "The Quorum Sensing Negative Regulators EsaR and ExpREcc, Homologues within the LuxR Family, Retain the Ability To Function as Activators of Transcription." Journal of Bacteriology 185, no. 23 (December 1, 2003): 7001–7. http://dx.doi.org/10.1128/jb.185.23.7001-7007.2003.

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ABSTRACT Most LuxR homologues function as activators of transcription during the process of quorum sensing, but a few, including EsaR and ExpR Ecc , negatively impact gene expression. The LuxR-activated luxI promoter and LuxR binding site, the lux box, were used in artificial contexts to assess the potential for transcriptional activation and DNA binding by EsaR and ExpR Ecc . Although the acyl-homoserine lactone responsiveness of both proteins is the opposite of that shown by most LuxR family members, EsaR and ExpR Ecc have preserved the ability to interact with RNA polymerase and activate tr

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8

Subramoni, Sujatha, and Vittorio Venturi. "LuxR-family ‘solos’: bachelor sensors/regulators of signalling molecules." Microbiology 155, no. 5 (May 1, 2009): 1377–85. http://dx.doi.org/10.1099/mic.0.026849-0.

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N-Acylhomoserine lactone (AHL) quorum-sensing (QS) signalling is the best-understood chemical language in proteobacteria. In the last 15 years a large amount of research in several bacterial species has revealed in detail the genetic, molecular and biochemical mechanisms underlying AHL signalling. These studies have revealed the role played by protein pairs of the AHL synthase belonging to the LuxI family and cognate LuxR-family AHL sensor–regulator. Proteobacteria however commonly possess a QS LuxR-family protein for which there is no obvious cognate LuxI synthase; these proteins are found in

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9

McDougald, Diane, Scott A. Rice, and Staffan Kjelleberg. "SmcR-Dependent Regulation of Adaptive Phenotypes inVibrio vulnificus." Journal of Bacteriology 183, no. 2 (January 15, 2001): 758–62. http://dx.doi.org/10.1128/jb.183.2.758-762.2001.

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ABSTRACT Vibrio vulnificus contains homologues of the V. harveyi luxR and luxS genes. A null mutation insmcR (luxR) resulted in a defect in starvation survival, inhibition of starvation-induced maintenance of culturability that occurs when V. vulnificusis starved prior to low-temperature incubation, and increased expression of stationary-phase phenotypes.

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Gray, Kendall M., and James R. Garey. "The evolution of bacterial LuxI and LuxR quorum sensing regulators." Microbiology 147, no. 8 (August 1, 2001): 2379–87. http://dx.doi.org/10.1099/00221287-147-8-2379.

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Brameyer, Sophie, Darko Kresovic, Helge B. Bode, and Ralf Heermann. "Dialkylresorcinols as bacterial signaling molecules." Proceedings of the National Academy of Sciences 112, no. 2 (December 30, 2014): 572–77. http://dx.doi.org/10.1073/pnas.1417685112.

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It is well recognized that bacteria communicate via small diffusible molecules, a process termed quorum sensing. The best understood quorum sensing systems are those that use acylated homoserine lactones (AHLs) for communication. The prototype of those systems consists of a LuxI-like AHL synthase and a cognate LuxR receptor that detects the signal. However, many proteobacteria possess LuxR receptors, yet lack any LuxI-type synthase, and thus these receptors are referred to as LuxR orphans or solos. In addition to the well-known AHLs, little is known about the signaling molecules that are sense

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12

Zheng, Huiming, Yiling Mao, Qingcheng Zhu, Jun Ling, Na Zhang, Nawar Naseer, Zengtao Zhong, and Jun Zhu. "The Quorum Sensing Regulator CinR Hierarchically Regulates Two Other Quorum Sensing Pathways in Ligand-Dependent and -Independent Fashions in Rhizobium etli." Journal of Bacteriology 197, no. 9 (February 17, 2015): 1573–81. http://dx.doi.org/10.1128/jb.00003-15.

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ABSTRACTMany rhizobial species use complexN-acyl-homoserine lactone (AHL)-based quorum sensing (QS) systems to monitor their population density and regulate their symbiotic interactions with their plant hosts. There are at least three LuxRI-type regulatory systems inRhizobium etliCFN42: CinRI, RaiRI, and TraRI. In this study, we show that CinI, RaiI, and TraI are responsible for synthesizing all AHLs under the tested conditions. The activation of these AHL synthase genes requires their corresponding LuxR-type counterparts. We further demonstrate that CinRI is at the top of the regulatory casca

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Truong, Thao T., Mohammad Seyedsayamdost, E. Peter Greenberg, and Josephine R. Chandler. "A Burkholderia thailandensis Acyl-Homoserine Lactone-Independent Orphan LuxR Homolog That Activates Production of the Cytotoxin Malleilactone." Journal of Bacteriology 197, no. 21 (August 17, 2015): 3456–62. http://dx.doi.org/10.1128/jb.00425-15.

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ABSTRACTBurkholderia thailandensishas three acyl-homoserine lactone (AHL) LuxR-LuxI quorum-sensing circuits and two orphan LuxR homologs. Orphans are LuxR-type transcription factors that do not have cognate LuxI-type AHL synthases. One of the orphans, MalR, is genetically linked to themalgene cluster, which encodes enzymes required for production of the cytotoxic polyketide malleilactone. Under normal laboratory conditions themalgene cluster is silent; however, antibiotics like trimethoprim inducemaltranscription. We show that trimethoprim-dependent induction of themalgenes requires MalR. MalR

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14

Nasuno, Eri, Nobutada Kimura, Masaki J. Fujita, Cindy H. Nakatsu, Yoichi Kamagata, and Satoshi Hanada. "Phylogenetically Novel LuxI/LuxR-Type Quorum Sensing Systems Isolated Using a Metagenomic Approach." Applied and Environmental Microbiology 78, no. 22 (September 14, 2012): 8067–74. http://dx.doi.org/10.1128/aem.01442-12.

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ABSTRACTA great deal of research has been done to understand bacterial cell-to-cell signaling systems, but there is still a large gap in our current knowledge because the majority of microorganisms in natural environments do not have cultivated representatives. Metagenomics is one approach to identify novel quorum sensing (QS) systems from uncultured bacteria in environmental samples. In this study, fosmid metagenomic libraries were constructed from a forest soil and an activated sludge from a coke plant, and the target genes were detected using a green fluorescent protein (GFP)-basedEscherich

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15

Plener, Laure, Nicola Lorenz, Matthias Reiger, Tiago Ramalho, Ulrich Gerland, and Kirsten Jung. "The Phosphorylation Flow of the Vibrio harveyi Quorum-Sensing Cascade Determines Levels of Phenotypic Heterogeneity in the Population." Journal of Bacteriology 197, no. 10 (March 9, 2015): 1747–56. http://dx.doi.org/10.1128/jb.02544-14.

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ABSTRACTQuorum sensing (QS) is a communication process that enables a bacterial population to coordinate and synchronize specific behaviors. The bioluminescent marine bacteriumVibrio harveyiintegrates three autoinducer (AI) signals into one quorum-sensing cascade comprising a phosphorelay involving three hybrid sensor kinases: LuxU; LuxO, an Hfq/small RNA (sRNA) switch; and the transcriptional regulator LuxR. Using a new set ofV. harveyimutants lacking genes for the AI synthases and/or sensors, we assayed the activity of the quorum-sensing cascade at the population and single-cell levels, with

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Rutherford, Steven T., Julie S. Valastyan, Thibaud Taillefumier, Ned S. Wingreen, and Bonnie L. Bassler. "Comprehensive analysis reveals how single nucleotides contribute to noncoding RNA function in bacterial quorum sensing." Proceedings of the National Academy of Sciences 112, no. 44 (October 19, 2015): E6038—E6047. http://dx.doi.org/10.1073/pnas.1518958112.

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Five homologous noncoding small RNAs (sRNAs), called the Qrr1-5 sRNAs, function in the Vibrio harveyi quorum-sensing cascade to drive its operation. Qrr1-5 use four different regulatory mechanisms to control the expression of ∼20 mRNA targets. Little is known about the roles individual nucleotides play in mRNA target selection, in determining regulatory mechanism, or in defining Qrr potency and dynamics of target regulation. To identify the nucleotides vital for Qrr function, we developed a method we call RSort-Seq that combines saturating mutagenesis, fluorescence-activated cell sorting, high

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White, Catharine E., and Stephen C. Winans. "Cell–cell communication in the plant pathogen Agrobacterium tumefaciens." Philosophical Transactions of the Royal Society B: Biological Sciences 362, no. 1483 (March 13, 2007): 1135–48. http://dx.doi.org/10.1098/rstb.2007.2040.

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The plant pathogen Agrobacterium tumefaciens induces the formation of crown gall tumours at wound sites on host plants by directly transforming plant cells. This disease strategy benefits the bacteria as the infected plant tissue produces novel nutrients, called opines, that the colonizing bacteria can use as nutrients. Almost all of the genes that are required for virulence, and all of the opine uptake and utilization genes, are carried on large tumour-inducing (Ti) plasmids. The observation more than 25 years ago that specific opines are required for Ti plasmid conjugal transfer led to the d

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Ulrich, Ricky L., David DeShazer, Harry B. Hines, and Jeffrey A. Jeddeloh. "Quorum Sensing: a Transcriptional Regulatory System Involved in the Pathogenicity of Burkholderia mallei." Infection and Immunity 72, no. 11 (November 2004): 6589–96. http://dx.doi.org/10.1128/iai.72.11.6589-6596.2004.

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ABSTRACT Numerous gram-negative bacterial pathogens regulate virulence factor expression by using a cell density mechanism termed quorum sensing (QS). An in silico analysis of the Burkholderia mallei ATCC 23344 genome revealed that it encodes at least two luxI and four luxR homologues. Using mass spectrometry, we showed that wild-type B. mallei produces the signaling molecules N-octanoyl-homoserine lactone and N-decanoyl-homoserine lactone. To determine if QS is involved in the virulence of B. mallei, we generated mutations in each putative luxIR homologue and tested the pathogenicities of the

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Hawkins, Andrew C., Frances H. Arnold, Rainer Stuermer, Bernhard Hauer, and Jared R. Leadbetter. "Directed Evolution of Vibrio fischeri LuxR for Improved Response to Butanoyl-Homoserine Lactone." Applied and Environmental Microbiology 73, no. 18 (August 3, 2007): 5775–81. http://dx.doi.org/10.1128/aem.00060-07.

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ABSTRACT LuxR is the 3-oxohexanoyl-homoserine lactone (3OC6HSL)-dependent transcriptional activator of the prototypical acyl-homoserine lactone (AHL) quorum-sensing system of Vibrio fischeri. Wild-type LuxR exhibits no response to butanoyl-HSL (C4HSL) in quantitative bioassays at concentrations of up to 1 μM; a previously described LuxR variant (LuxR-G2E) exhibits a broadened response to diverse AHLs, including pentanoyl-HSL (C5HSL), but not to C4HSL. Here, two rounds of directed evolution of LuxR-G2E generated variants of LuxR that responded to C4HSL at concentrations as low as 10 nM. One var

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20

Perry, Lynda L., Nathan G. Bright, Richard J. Carroll, Jr., M. Cathy Scott, Michael S. Allen, and Bruce M. Applegate. "Molecular characterization of autoinduction of bioluminescence in the Microtox® indicator strain Vibrio fischeri ATCC 49387." Canadian Journal of Microbiology 51, no. 7 (July 1, 2005): 549–57. http://dx.doi.org/10.1139/w05-019.

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Repeated attempts to clone the luxI from Vibrio fischeri ATCC 49387 failed to produce a clone carrying a functional LuxI. Sequence data from the clones revealed the presence of a polymorphism when compared with previously published luxI sequences, prompting further characterization of bioluminescence regulation in V. fischeri ATCC 49387. Further investigation of V. fischeri ATCC 49387 revealed that its LuxI protein lacks detectable LuxI activity due to the presence of a glutamine residue at position 125 in the deduced amino acid sequence. Specific bioluminescence in V. fischeri ATCC 49387 incr

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Schu, Daniel J., Aurelien L. Carlier, Katherine P. Jamison, Susanne von Bodman, and Ann M. Stevens. "Structure/Function Analysis of the Pantoea stewartii Quorum-Sensing Regulator EsaR as an Activator of Transcription." Journal of Bacteriology 191, no. 24 (October 9, 2009): 7402–9. http://dx.doi.org/10.1128/jb.00994-09.

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ABSTRACT In Pantoea stewartii subsp. stewartii, two regulatory proteins are key to the process of cell-cell communication known as quorum sensing: the LuxI and LuxR homologues EsaI and EsaR. Most LuxR homologues function as activators of transcription in the presence of their cognate acylated homoserine lactone (AHL) signal. However, EsaR was initially found to function as a repressor in the absence of AHL. Previous studies demonstrated that, in the absence of AHL, EsaR retains the ability to function as a weak activator of the lux operon in recombinant Escherichia coli. Here it is shown that

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Grellier, N., M. Suzuki, L. Brot, A. Rodrigues, L. Humbert, K. Escoubeyrou, D. Rainteau, J. P. Grill, R. Lami, and P. Seksik. "DOP50 Impact of Inflammatory Bowel Disease associated dysbiosis on bacterial quorum sensing mediated by Acyl-homoserine Lactone in human gut microbiota." Journal of Crohn's and Colitis 17, Supplement_1 (January 30, 2023): i119—i121. http://dx.doi.org/10.1093/ecco-jcc/jjac190.0090.

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Abstract Background Intestinal dysbiosis is a key feature in the pathogenesis of inflammatory bowel diseases (IBD). Bacterial quorum sensing mediated by acyl-homoserine lactones (AHL) might play a role in the dialogue between the gut microbiota and the host. The main objective of our study was to investigate the presence and expression of AHL synthase and receptor genes in the human gut ecosystem during IBD. Methods To confirm the presence of AHL in the gut, mass spectrometric detection was performed on stool samples from IBD patients and non-IBD subjects. Then, by an in silico approach, we ex

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Callahan, Sean M., and Paul V. Dunlap. "LuxR- and Acyl-Homoserine-Lactone-Controlled Non-luxGenes Define a Quorum-Sensing Regulon in Vibrio fischeri." Journal of Bacteriology 182, no. 10 (May 15, 2000): 2811–22. http://dx.doi.org/10.1128/jb.182.10.2811-2822.2000.

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ABSTRACT The luminescence (lux) operon (luxICDABEG) of the symbiotic bacterium Vibrio fischeri is regulated by the transcriptional activator LuxR and two acyl-homoserine lactone (acyl-HSL) autoinducers (the luxI-dependent 3-oxo-hexanoyl-HSL [3-oxo-C6-HSL] and the ainS-dependent octanoyl-HSL [C8-HSL]) in a population density-responsive manner called quorum sensing. To identify quorum-sensing-regulated (QSR) proteins different from those encoded by lux genes, we examined the protein patterns of V. fischeri quorum-sensing mutants defective in luxI, ainS, andluxR by two-dimensional polyacrylamide

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Shadel, G. S., and T. O. Baldwin. "Positive autoregulation of the Vibrio fischeri luxR gene. LuxR and autoinducer activate cAMP-catabolite gene activator protein complex-independent and -dependent luxR transcription." Journal of Biological Chemistry 267, no. 11 (April 1992): 7696–702. http://dx.doi.org/10.1016/s0021-9258(18)42571-9.

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Zhang, Jun, Bing Liu, Dan Gu, Yuan Hao, Mo Chen, Yue Ma, Xiaohui Zhou, David Reverter, Yuanxing Zhang, and Qiyao Wang. "Binding site profiles and N-terminal minor groove interactions of the master quorum-sensing regulator LuxR enable flexible control of gene activation and repression." Nucleic Acids Research 49, no. 6 (March 8, 2021): 3274–93. http://dx.doi.org/10.1093/nar/gkab150.

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Abstract LuxR is a TetR family master quorum sensing (QS) regulator activating or repressing expression of hundreds of genes that control collective behaviors in Vibrios with underlying mechanism unknown. To illuminate how this regulator controls expression of various target genes, we applied ChIP-seq and DNase I-seq technologies. Vibrio alginolyticus LuxR controls expression of ∼280 genes that contain either symmetric palindrome (repDNA) or asymmetric (actDNA) binding motifs with different binding profiles. The median number of LuxR binding sites for activated genes are nearly double for that

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Trott, Amy E., and Ann M. Stevens. "Amino Acid Residues in LuxR Critical for Its Mechanism of Transcriptional Activation during Quorum Sensing inVibrio fischeri." Journal of Bacteriology 183, no. 1 (January 1, 2001): 387–92. http://dx.doi.org/10.1128/jb.183.1.387-392.2001.

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ABSTRACT PCR-based site-directed mutagenesis has been used to generate 38 alanine-substitution mutations in the C-terminal 41 amino acid residues of LuxR. This region plays a critical role in the mechanism of LuxR-dependent transcriptional activation of the Vibrio fischeri lux operon during quorum sensing. The ability of the variant forms of LuxR to activate transcription of the lux operon was examined by using in vivo assays in recombinant Escherichia coli. Eight recombinant strains produced luciferase at levels less than 50% of that of a strain expressing wild-type LuxR. Western immunoblotti

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Egland, Kristi A., and E. P. Greenberg. "Conversion of the Vibrio fischeriTranscriptional Activator, LuxR, to a Repressor." Journal of Bacteriology 182, no. 3 (February 1, 2000): 805–11. http://dx.doi.org/10.1128/jb.182.3.805-811.2000.

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ABSTRACT The Vibrio fischeri luminescence (lux) operon is regulated by a quorum-sensing system that involves the transcriptional activator (LuxR) and an acyl-homoserine lactone signal. Transcriptional activation requires the presence of a 20-base inverted repeat termed the lux box at a position centered 42.5 bases upstream of the transcriptional start of the lux operon. LuxR has proven difficult to study in vitro. A truncated form of LuxR has been purified, and together with ς70 RNA polymerase it can activate transcription of the lux operon. Both the truncated LuxR and RNA polymerase are requi

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Li, Shuangjia, Shijun Wu, Yixuan Ren, Qiu Meng, Jianhua Yin, and Zhiliang Yu. "Characterization of differentiated autoregulation of LuxI/LuxR-type quorum sensing system in Pseudoalteromonas." Biochemical and Biophysical Research Communications 590 (January 2022): 177–83. http://dx.doi.org/10.1016/j.bbrc.2021.12.107.

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Lau, Yin Yin, Kah Yan How, Wai-Fong Yin, and Kok-Gan Chan. "Functional characterization of quorum sensing LuxR-type transcriptional regulator, EasR in Enterobacter asburiae strain L1." PeerJ 8 (October 21, 2020): e10068. http://dx.doi.org/10.7717/peerj.10068.

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Over the past decades, Enterobacter spp. have been identified as challenging and important pathogens. The emergence of multidrug-resistant Enterobacteria especially those that produce Klebsiella pneumoniae carbapenemase has been a very worrying health crisis. Although efforts have been made to unravel the complex mechanisms that contribute to the pathogenicity of different Enterobacter spp., there is very little information associated with AHL-type QS mechanism in Enterobacter spp. Signaling via N-acyl homoserine lactone (AHL) is the most common quorum sensing (QS) mechanism utilized by Proteo

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Duerkop, Breck A., Ricky L. Ulrich, and E. Peter Greenberg. "Octanoyl-Homoserine Lactone Is the Cognate Signal for Burkholderia mallei BmaR1-BmaI1 Quorum Sensing." Journal of Bacteriology 189, no. 14 (May 11, 2007): 5034–40. http://dx.doi.org/10.1128/jb.00317-07.

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ABSTRACT Acyl-homoserine lactones (HSLs) serve as quorum-sensing signals for many Proteobacteria. Members of the LuxI family of signal generators catalyze the production of acyl-HSLs, which bind to a cognate receptor in the LuxR family of transcription factors. The obligate animal pathogen Burkholderia mallei produces several acyl-HSLs, and the B. mallei genome has four luxR and two luxI homologs, each of which has been established as a virulence factor. To begin to delineate the relevant acyl-HSL signals for B. mallei LuxR homologs, we analyzed the BmaR1-BmaI1 system. A comparison of acyl-HSL

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Chandler, Josephine R., Breck A. Duerkop, Aaron Hinz, T. Eoin West, Jake P. Herman, Mair E. A. Churchill, Shawn J. Skerrett, and E. Peter Greenberg. "Mutational Analysis of Burkholderia thailandensis Quorum Sensing and Self-Aggregation." Journal of Bacteriology 191, no. 19 (July 31, 2009): 5901–9. http://dx.doi.org/10.1128/jb.00591-09.

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ABSTRACT Acyl-homoserine lactone (acyl-HSL) quorum-sensing signaling is common to many Proteobacteria. Acyl-HSLs are synthesized by the LuxI family of synthases, and the signal response is mediated by members of the LuxR family of transcriptional regulators. Burkholderia thailandensis is a member of a closely related cluster of three species, including the animal pathogens Burkholderia mallei and Burkholderia pseudomallei. Members of this group have similar luxI and luxR homologs, and these genes contribute to B. pseudomallei and B. mallei virulence. B. thailandensis possesses three pairs of l

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32

Joshi, Janak Raj, Netaly Khazanov, Amy Charkowski, Adi Faigenboim, Hanoch Senderowitz, and Iris Yedidia. "Interkingdom Signaling Interference: The Effect of Plant-Derived Small Molecules on Quorum Sensing in Plant-Pathogenic Bacteria." Annual Review of Phytopathology 59, no. 1 (August 25, 2021): 153–90. http://dx.doi.org/10.1146/annurev-phyto-020620-095740.

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In the battle between bacteria and plants, bacteria often use a population density–dependent regulatory system known as quorum sensing (QS) to coordinate virulence gene expression. In response, plants use innate and induced defense mechanisms that include low-molecular-weight compounds, some of which serve as antivirulence agents by interfering with the QS machinery. The best-characterized QS system is driven by the autoinducer N-acyl-homoserine lactone (AHL), which is produced by AHL synthases (LuxI homologs) and perceived by response regulators (LuxR homologs). Several plant compounds have b

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33

Sayut, Daniel J., Yan Niu, and Lianhong Sun. "Construction and Enhancement of a Minimal Genetic AND Logic Gate." Applied and Environmental Microbiology 75, no. 3 (December 5, 2008): 637–42. http://dx.doi.org/10.1128/aem.01684-08.

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ABSTRACT The ability of genetic networks to integrate multiple inputs in the generation of cellular responses is critical for the adaptation of cellular phenotype to distinct environments and of great interest in the construction of complex artificial circuits. To develop artificial genetic circuits that can integrate intercellular signaling molecules and commonly used inducing agents, we have constructed an artificial genetic AND gate based on the P luxI quorum-sensing promoter and the lac repressor. The hybrid promoter exhibited reduced basal and induced expression levels but increased expre

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34

Finney, Angela H., Robert J. Blick, Katsuhiko Murakami, Akira Ishihama, and Ann M. Stevens. "Role of the C-Terminal Domain of the Alpha Subunit of RNA Polymerase in LuxR-Dependent Transcriptional Activation of the lux Operon during Quorum Sensing." Journal of Bacteriology 184, no. 16 (August 15, 2002): 4520–28. http://dx.doi.org/10.1128/jb.184.16.4520-4528.2002.

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ABSTRACT During quorum sensing in Vibrio fischeri, the luminescence, or lux, operon is regulated in a cell density-dependent manner by the activator LuxR in the presence of an acylated homoserine lactone autoinducer molecule [N-(3-oxohexanoyl) homoserine lactone]. LuxR, which binds to the lux operon promoter at a position centered at −42.5 relative to the transcription initiation site, is thought to function as an ambidextrous activator making multiple contacts with RNA polymerase (RNAP). The specific role of the α-subunit C-terminal domain (αCTD) of RNAP in LuxR-dependent transcriptional acti

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35

Zheng, Huiming, Zengtao Zhong, Xin Lai, Wen-Xin Chen, Shunpeng Li, and Jun Zhu. "A LuxR/LuxI-Type Quorum-Sensing System in a Plant Bacterium, Mesorhizobium tianshanense, Controls Symbiotic Nodulation." Journal of Bacteriology 188, no. 5 (March 1, 2006): 1943–49. http://dx.doi.org/10.1128/jb.188.5.1943-1949.2006.

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ABSTRACT The ability of rhizobia to symbiotically fix nitrogen from the atmosphere when forming nodules on their plant hosts requires various signal transduction pathways. LuxR-LuxI-type quorum-sensing systems have been shown to be one of the players in a number of rhizobium species. In this study, we found that Mesorhizobium tianshanense, a moderate-growth Rhizobium that forms nodules on a number of licorice plants, produces multiple N-acyl homoserine lactone (AHL)-like molecules. A simple screen for AHL synthase genes using an M. tianshanense genomic expression library in Escherichia coli, c

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36

Qin, Nan, Sean M. Callahan, Paul V. Dunlap, and Ann M. Stevens. "Analysis of LuxR Regulon Gene Expression during Quorum Sensing in Vibrio fischeri." Journal of Bacteriology 189, no. 11 (March 30, 2007): 4127–34. http://dx.doi.org/10.1128/jb.01779-06.

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ABSTRACT The regulation of the lux operon (luxICDABEG) of Vibrio fischeri has been intensively studied as a model for quorum sensing in proteobacteria. Two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis previously identified several non-Lux proteins in V. fischeri MJ-100 whose expression was dependent on LuxR and 3-oxo-hexanoyl-l-homoserine lactone (3-oxo-C6-HSL). To determine if the LuxR-dependent regulation of the genes encoding these proteins was due to direct transcriptional control by LuxR and 3-oxo-C6-HSL or instead was due to indirect control via an unide

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37

Egland, Kristi A., and E. P. Greenberg. "Quorum Sensing in Vibrio fischeri: Analysis of the LuxR DNA Binding Region by Alanine-Scanning Mutagenesis." Journal of Bacteriology 183, no. 1 (January 1, 2001): 382–86. http://dx.doi.org/10.1128/jb.183.1.382-386.2001.

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ABSTRACT LuxR is the transcriptional activator for quorum-sensing control of luminescence in Vibrio fischeri. A series of alanine-scanning mutants spanning a predicted helix-turn-helix region in the DNA binding domain of LuxR was constructed, and the activity of each of the LuxR mutant proteins in recombinant Escherichia coli was investigated. The region covered by the mutagenesis spanned residues 190 to 224. About half of the alanine-scanning mutants showed activities similar to that of the wild-type LuxR: at least two were positive-control mutants, four appeared to be defective in DNA bindin

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38

Chatterjee, Jaidip, Carol M. Miyamoto, and Edward A. Meighen. "Autoregulation of luxR: the Vibrio harveyi lux-operon activator functions as a repressor." Molecular Microbiology 20, no. 2 (April 1996): 415–25. http://dx.doi.org/10.1111/j.1365-2958.1996.tb02628.x.

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39

Patankar, Arati V., and Juan E. González. "Orphan LuxR regulators of quorum sensing." FEMS Microbiology Reviews 33, no. 4 (July 2009): 739–56. http://dx.doi.org/10.1111/j.1574-6976.2009.00163.x.

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40

Wu, Shijun, Shuangjia Li, Jianhua Yin, and Zhiliang Yu. "Hfq and sRNA00002 positively regulate the LuxI/LuxR-type quorum sensing system in Pseudoalteromonas." Biochemical and Biophysical Research Communications 571 (September 2021): 1–7. http://dx.doi.org/10.1016/j.bbrc.2021.07.058.

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41

Fuqua, W. C., S. C. Winans, and E. P. Greenberg. "Quorum sensing in bacteria: the LuxR-LuxI family of cell density-responsive transcriptional regulators." Journal of Bacteriology 176, no. 2 (1994): 269–75. http://dx.doi.org/10.1128/jb.176.2.269-275.1994.

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42

Hao, Youai, Stephen C. Winans, Bernard R. Glick, and Trevor C. Charles. "Identification and characterization of new LuxR/LuxI-type quorum sensing systems from metagenomic libraries." Environmental Microbiology 12, no. 1 (January 2010): 105–17. http://dx.doi.org/10.1111/j.1462-2920.2009.02049.x.

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43

Stevens, Ann M., Nobuyuki Fujita, Akira Ishihama та E. P. Greenberg. "Involvement of the RNA Polymerase α-Subunit C-Terminal Domain in LuxR-Dependent Activation of the Vibrio fischeri Luminescence Genes". Journal of Bacteriology 181, № 15 (1 серпня 1999): 4704–7. http://dx.doi.org/10.1128/jb.181.15.4704-4707.1999.

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Abstract (sommario):

ABSTRACT LuxR is a ς70 RNA polymerase (RNAP)-dependent transcriptional activator that controls expression of the Vibrio fischeri lux operon in response to an acylhomoserine lactone-cell density signal. We have investigated whether the α-subunit C-terminal domain (αCTD) of RNAP is required for LuxR activity. A purified signal-independent, LuxR C-terminal domain-containing polypeptide (LuxRΔN) was used to study the activation of transcription from theluxI promoter in vitro. Initiation of luxoperon transcription was observed in the presence of LuxRΔN and wild-type RNAP but not in the presence of

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44

Costa, José M., and Joyce E. Loper. "Ecbl and EcbR: homologs of Luxl and LuxR affecting antibiotic and exoenzyme production byErwinia carotovorasubsp.betavasculorum." Canadian Journal of Microbiology 43, no. 12 (December 1, 1997): 1164–71. http://dx.doi.org/10.1139/m97-165.

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Erwinia carotovora subsp. betavasculorum Ecb168 causes vascular necrosis and root rot of sugar beet and produces an antibiotic(s) that is antagonistic against other Erwinia spp. EcbI−mutants of Ecb168, each containing a single transposon insertion in the ecbI gene (for Erwinia carotovora subsp. betavasculorum inducer), do not produce detectable levels of extracellular protease or antibiotic(s), and express less pectate lyase activity and virulence than the wild-type strain. A plasmid containing the cloned ecbI gene complemented the EcbI−mutants for these phenotypes. Protease production by EcbI

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45

Manukhov, Ilya V., Ol'ga E. Melkina, Ignatii I. Goryanin, Ancha V. Baranova, and Gennadii B. Zavilgelsky. "The N-Terminal Domain of Aliivibrio fischeri LuxR Is a Target of the GroEL Chaperonin." Journal of Bacteriology 192, no. 20 (August 20, 2010): 5549–51. http://dx.doi.org/10.1128/jb.00754-10.

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Abstract (sommario):

ABSTRACT Here we show that the C-terminal domain of LuxR activates the transcription of Aliivibrio fischeri luxICDABEG in Escherichia coli SKB178 gro + and E. coli OFB1111 groEL673 strains to the same level. Using affinity chromatography, we showed that GroEL binds to the N-terminal domain of LuxR, pointing to a GroEL/GroES requirement for the folding of the N-terminal domain of LuxR.

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46

Kviatkovski, I., T. Yarnitzky, S. Shushan, O. Schwartz-Harari, R. Nir-Paz, and Y. Helman. "A bacterial biosensor encoding a genetically modified LuxR receptor exhibits improved detection of Pseudomonas aeruginosa's biomarker molecule 2-aminoacetophenone." Chemical Communications 54, no. 66 (2018): 9218–21. http://dx.doi.org/10.1039/c8cc03540g.

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47

Malott, Rebecca J., Eoin P. O'Grady, Jessica Toller, Silja Inhülsen, Leo Eberl, and Pamela A. Sokol. "A Burkholderia cenocepacia Orphan LuxR Homolog Is Involved in Quorum-Sensing Regulation." Journal of Bacteriology 191, no. 8 (February 6, 2009): 2447–60. http://dx.doi.org/10.1128/jb.01746-08.

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Abstract (sommario):

ABSTRACT Burkholderia cenocepacia utilizes quorum sensing to control gene expression, including the expression of genes involved in virulence. In addition to CepR and CciR, a third LuxR homolog, CepR2, was found to regulate gene expression and virulence factor production. All B. cenocepacia strains examined contained this orphan LuxR homolog, which was not associated with an adjacent N-acyl-homoserine lactone synthase gene. Expression of cepR2 was negatively autoregulated and was negatively regulated by CciR in strain K56-2. Microarray analysis and quantitative reverse transcription-PCR determ

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48

Freeman, Jeremy A., and Bonnie L. Bassler. "Sequence and Function of LuxU: a Two-Component Phosphorelay Protein That Regulates Quorum Sensing inVibrio harveyi." Journal of Bacteriology 181, no. 3 (February 1, 1999): 899–906. http://dx.doi.org/10.1128/jb.181.3.899-906.1999.

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Abstract (sommario):

ABSTRACT Vibrio harveyi regulates the expression of bioluminescence (lux) in response to cell density, a phenomenon known as quorum sensing. In V. harveyi, two independent quorum-sensing systems exist, and each produces, detects, and responds to a specific cell density-dependent autoinducer signal. The autoinducers are recognized by two-component hybrid sensor kinases called LuxN and LuxQ, and sensory information from both systems is transduced by a phosphorelay mechanism to the response regulator protein LuxO. Genetic evidence suggests that LuxO-phosphate negatively regulates the expression o

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49

Fuqua, Clay, Stephen C. Winans, and E. Peter Greenberg. "CENSUS AND CONSENSUS IN BACTERIAL ECOSYSTEMS: The LuxR-LuxI Family of Quorum-Sensing Transcriptional Regulators." Annual Review of Microbiology 50, no. 1 (October 1996): 727–51. http://dx.doi.org/10.1146/annurev.micro.50.1.727.

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50

Miyamoto, Carol M., Paul V. Dunlap, Edward G. Ruby, and Edward A. Meighen. "LuxO controls luxR expression in Vibrio harveyi: evidence for a common regulatory mechanism in Vibrio." Molecular Microbiology 48, no. 2 (April 4, 2003): 537–48. http://dx.doi.org/10.1046/j.1365-2958.2003.03453.x.

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