Letteratura scientifica selezionata sul tema "Liquid/liquid extraction purification"
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Articoli di riviste sul tema "Liquid/liquid extraction purification"
Huseynov, H. D. "IONIC LIQUID EXTRACTION CLEANING OF PETROLEUM FRACTIONS". Chemical Problems 20, n. 3 (2022): 197–212. http://dx.doi.org/10.32737/2221-8688-2022-3-197-212.
Testo completoIbragimova, M. J., S. A. Seidova, S. G. Alieva, H. J. Huseynov, Kh A. Abdullaeva e F. Yu Yusifzade. "IONIC LIQUIDS AS EXTRAGENTS OF COMPOUNDS NEGATIVELY AFFECTING THE ENVIRONMENTAL PROPERTIES OF DIESEL FUEL". Azerbaijan Chemical Journal, n. 3 (21 agosto 2024): 20–28. http://dx.doi.org/10.32737/0005-2531-2024-3-20-28.
Testo completoBlaga, Alexandra Cristina, Alexandra Tucaliuc e Lenuta Kloetzer. "Applications of Ionic Liquids in Carboxylic Acids Separation". Membranes 12, n. 8 (9 agosto 2022): 771. http://dx.doi.org/10.3390/membranes12080771.
Testo completoEvstaf’ev, Sergei N., e Cuong Q. Hoang. "PURIFICATION OF 1-BUTYL-3-METHYLIMIDAZOLIUM CHLORIDE AFTER DISSOLUTION OF WHEAT STRAW". IZVESTIYA VYSSHIKH UCHEBNYKH ZAVEDENIY KHIMIYA KHIMICHESKAYA TEKHNOLOGIYA 61, n. 3 (27 febbraio 2018): 83. http://dx.doi.org/10.6060/tcct.20186103.5615.
Testo completoRen, Guangwei, Xingwen Gong, Bilei Wang, Yingyi Chen e Jianying Huang. "Affinity ionic liquids for the rapid liquid–liquid extraction purification of hexahistidine tagged proteins". Separation and Purification Technology 146 (maggio 2015): 114–20. http://dx.doi.org/10.1016/j.seppur.2015.03.025.
Testo completoCurran, Dennis P, Reena Bajpai e Elizabeth Sanger. "Purification of Fluorous Mitsunobu Reactions by Liquid-Liquid Extraction". Advanced Synthesis & Catalysis 348, n. 12-13 (agosto 2006): 1621–24. http://dx.doi.org/10.1002/adsc.200606187.
Testo completoBendada, A., A. H. Meniai e L. M. Bencheikh. "Modeling of Phosphoric Acid Purification by Liquid-Liquid Extraction". Chemical Engineering & Technology 24, n. 12 (dicembre 2001): 1273–80. http://dx.doi.org/10.1002/1521-4125(200112)24:12<1273::aid-ceat1273>3.0.co;2-j.
Testo completoBéri, János, Sára Nagy, Ádám Kolay Kovács, Erika Vági e Edit Székely. "Pressurized Liquid Extraction of Hemp Residue and Purification of the Extract with Liquid-Liquid Extraction". Periodica Polytechnica Chemical Engineering 66, n. 1 (26 novembre 2021): 82–90. http://dx.doi.org/10.3311/ppch.18456.
Testo completoLestari, Gabriella, Alinaghi Salari, Milad Abolhasani e Eugenia Kumacheva. "A microfluidic study of liquid–liquid extraction mediated by carbon dioxide". Lab on a Chip 16, n. 14 (2016): 2710–18. http://dx.doi.org/10.1039/c6lc00597g.
Testo completoNiu, Rui Xia, Bo Lin Li e Biao Long. "Purification and Fractionation of Natural Petroleum Sulfonates by Liquid-Liquid & Soxhlet Extraction". Advanced Materials Research 455-456 (gennaio 2012): 496–502. http://dx.doi.org/10.4028/www.scientific.net/amr.455-456.496.
Testo completoTesi sul tema "Liquid/liquid extraction purification"
Pinheiro, Regiane Silva. "Study of liquid-liquid extraction for methyl biodiesel purification process". Universidade Federal do CearÃ, 2013. http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=14103.
Testo completoIn the process of purification of biodiesel is of extreme importance washing methyl or ethyl ester rich- phase, since in conventional processes there is a great amount of water. During the washing is extracted primarily excess alcohol. Composition data for the washing process of biodiesel is still scarce in the literature and the study of liquid-liquid equilibrium provides the means to develop equipment and to optimize the extraction processes. Thus the main goal of this work is to determine equilibrium data for three different ternary systems: water - methanol - soybean biodiesel; corn biodiesel â methanol â water; methanol - coconut biodiesel - water at 20 Â C and 40 Â C. The experiments were carried out in equilibrium cells where the temperature was kept constant by thermostat control. The experimental determination was made by the method of densimetry. The consistency of data obtained for the tie lines was verified by correlations. It was verified by the ternary graphs, little influence of temperature on the systems studied. The equilibrium data were correlated liquid-liquid models for the activity coefficient NRTL, UNIQUAC and UNIFAC and proved quite satisfactory.
No processo de purificaÃÃo do biodiesel, a lavagem da fase rica em metil ou etil Ãster à de extrema importÃncia, visto que nos processos convencionais hà um grande gasto de Ãgua. Durante a lavagem à extraÃdo principalmente Ãlcool em excesso. Dados de composiÃÃes para a lavagem de biodiesel ainda sÃo escassos na literatura e o estudo do equilÃbrio lÃquido-lÃquido pode fornecer meios para o desenvolvimento de equipamentos e otimizaÃÃo dos processos de extraÃÃo. Dessa forma, o principal objetivo dessa dissertaÃÃo foi determinar dados de equilÃbrio lÃquido- lÃquido para os sistemas ternÃrios contendo biodiesel de soja + metanol + Ãgua, biodiesel de milho + metanol e Ãgua e biodiesel de coco + metanol + Ãgua, a 20 ÂC e 40 ÂC. Os experimentos foram feitos em cÃlulas de equilÃbrio mantendo a temperatura constante. A determinaÃÃo experimental das misturas ternÃrias foi feita pelos mÃtodos de titulaÃÃo e densimetria. A consistÃncia dos dados das linhas de amarraÃÃo foi verificada pelas correlaÃÃes de Othmer-Tobias e Hand. Verificou-se por meio de grÃficos ternÃrios, pouca influÃncia da temperatura sobre os sistemas estudados. Os dados de equilÃbrio lÃquido-lÃquido foram correlacionados pelos modelos para coeficiente de atividade NRTL, UNIQUAC e UNIFAC e mostraram-se bastante satisfatÃrios.
Campos, Assunção Mariana. "Etude physicochimique et formulation d'un nouveau solvant d'extraction pour la purification de l'acide phosphorique". Thesis, Paris 6, 2017. http://www.theses.fr/2017PA066045/document.
Testo completoThis PhD thesis was focused on the development of new extraction systems to purify phosphoric acid from wet phosphoric acid (WPA). The new system should be respectful of the current Prayon’s process flowsheet designed for the use of the mixture tri-n-butyl phosphate(TBP) and diisopropyl ether (DiPE) as extraction solvent. Firstly, extractants exhibiting different structures and functional groups were screened in order to assess their efficiency and selectivity towards phosphoric acid. Three new promising extraction systems were identified: diisobutyl ether (DBE), diisocarbinol (DiBC) in DiPE, and Fentamine T0810 (ATS) in DiPE. Secondly, our effort was directed to the comprehension of the physico-chemical phenomena involved in the extraction of phosphoric acid by the mixture TBP/DiPE at first, and enriched to describe the other systems studied later. Finally, a simulation model describing the solvent extraction of phosphoric acid by the solvent currently employed by Prayon is presented. This model accounts for the significant volume variation during the extraction of phosphoric acid and allows performing optimizations of extraction parameters even at very high phosphoric acid concentrations (6-14M)
Hewitson, Peter. "Intermittent counter-current extraction : a new continuous dynamic liquid-liquid extraction methodology". Thesis, Brunel University, 2014. http://bura.brunel.ac.uk/handle/2438/13900.
Testo completoLane, Marshalle. "Dispersive liquid-liquid micro-extraction coupled with gas chromatography for the detection of trihalomethanes in different water sources in the Western Cape, South Africa". Thesis, Cape Peninsula University of Technology, 2018. http://hdl.handle.net/20.500.11838/2852.
Testo completoTrihalomethanes (THMs) are a group of four compounds that are formed, along with other disinfected by-products. This happens when chloride or other disinfectants are used to control microbial contamination in drinking water, which then reacts with natural organic or inorganic substances in water. Trihalomethanes are better known by their common names such as chloroform, bromodichloromethane, chlorodibromomethane and bromoform. These four compounds are known to be classified as cancer group B carcinogens (shown to cause cancer in laboratory animals). Trihalomethane levels tend to increase with pH, temperature, time and the level of “precursors" present. Precursors are known to be organic substances which react with chloride to form THMs. One significant way of reducing the amount of THMs in water is to eliminate or reduce chlorination before filtrations and reduce precursors. There are guideline limits for THMs in the SANS 241:2015 document, but they are not continuously monitored and their levels in natural water are not known. The aim of this study is to develop a rapid, fast and reliable liquid-liquid microextraction technique, to determine the presence of THMs in natural water sources. This study particularly focuses on different water sources e.g. river, underground, borehole and chlorinated water. Chlorinated water is the water that has been presumably treated for bacteria and fungus growth. The results that were obtained for chlorinated water are as follow, 10.120 μg/L − 11.654 μg/L for chloroform, 2.214 μg/L - 2.666 μg/L for bromodichloromethane, 0.819 μg/L − 0.895 μg/L chlorodibromomethane and 0.103 μg/L - 0.135 μg/L for bromoform from validation data. All these THMs concentrations have been found to be below the SANS 241:2015 limits. Natural water shows a very high affinity for chloroform. This is what is expected under normal conditions as chloroform is the most abundant THM of all THMs present in natural water. The liquid-liquid microextraction technique that was optimized and used for the determination of THMs in this study is a rapid, simple and inexpensive technique that provides low limits of detection (LOD) e.g. 0.1999 μg/L chlorodibromomethane and 0.2056 μg/L bromoform and wide dynamic range (LOQ) of 0.6664 μg/L chlorodibromomethane and 0.6854 μg/L bromoform for the determination of THMs.
Mouhib, Mohamed. "Equilibres entre phases liquides et solides dans les systèmes H₂O-H₃PO₄-Solvant (DIPE ; TBP ; MIBK ; Hexane) en vue de la purification de l’acide phosphorique : Étude expérimentale et modélisation thermodynamique". Electronic Thesis or Diss., Lyon 1, 2024. http://www.theses.fr/2024LYO10276.
Testo completoPhosphoric acid (H3PO4) is the second most widely produced inorganic acid in the world, mainly used in fertilizer manufacturing. However, its use in advanced technological applications, such as the semiconductor industry, requires extremely high purity, which can only be achieved after extensive removal of dissolved metal cations and organic matter, from the original phosphate rock. To optimize the purification of wet-process phosphoric acid (WPA), it is essential to master the phase equilibria involved in two complementary and effective methods: liquid-liquid extraction and crystallization. In this context, the present work focuses on the systematic study and modeling of liquid-liquid, solid-liquid, and solid-liquid-liquid phase equilibria involving phosphoric acid, water, and various organic solvents. First part presents a systematic liquid-liquid equilibrium analysis of quaternary systems (H2O-H3PO4-DIPE-TBP, H2O-H3PO4-DIPE-MIBK, and H2O-H3PO4-TBP-MIBK) to assess the synergistic effect of these mixed solvents on phosphoric acid extraction efficiency. In addition, in the ternary H2O-H3PO4-DIPE system, the efficiency was also evaluated taking into account the influence of the addition of TBP and MIBK solvents on the formation of a third liquid phase. The second part focuses on the study of solid-liquid equilibria in the H2O-P2O5 system, including the study of the binary systems H2O-H3PO4 and H3PO4-H4P2O7 by solubility measurements, calorimetry and X-ray diffraction. This study delimits the stability domains of phosphoric acid and its hydrates, and highlights the existence of new stable, metastable phases, as well as possible polymorphisms. Then, the Quasi-ideal model was used to refine the experimental data and obtain information on the structure of the solutions. Finally, the third part explores the solid-liquid-liquid equilibria (SLLE) of the H2O-H3PO4-C6H14 system as a function of temperature. Experimental data was modeled from 283.2 K to the melting temperature of anhydrous phosphoric acid, providing an accurate representation of demixing equilibria and SLLE throughout the composition range
Alenazi, Mohrah. "Extraction and Purification of Biologically Active Metabolites from Rhodococcus sp. MTM3W5.2". Digital Commons @ East Tennessee State University, 2018. https://dc.etsu.edu/etd/3507.
Testo completoGomes, Joana Margarida Mota. "Purification of IgY using aqueous biphasic systems composed of good’s buffers ionic liquids". Master's thesis, Universidade de Aveiro, 2015. http://hdl.handle.net/10773/15391.
Testo completoThe increased inefficiency of antibiotics observed in the past few years, derived from the growing number of drug-resistant organisms and the appearance of individuals with impaired immune system, led to a significant research on antibodies for use in passive immunotherapy. Antibodies obtained from the egg yolk of immunized hens, immunoglobulin Y (IgY), are a promising alternative to mammalian antibodies. They can be obtained in higher titres and by less invasive techniques, thus opening the door for a new kind of more economic biopharmaceuticals. However, the available techniques for the IgY extraction and purification are time-consuming, labor intensive, low yielding, provide low-purification levels, are of high cost and cannot easily be scaled-up for industrial applications. In this work, ABS composed of C6H5K3O7 and ILs synthetized by the combination of anions with buffer capacity (Good’s buffers), with the tetrabutylphosphonium ([P4444]+) and tetrabutylammonium ([N4444]+) cations, were investigated. The use of different ILs allowed the study of the cation and anion nature on the phase diagrams behaviour and thus on their ability to form ABS. Further, the ability of these systems as an alternative purification platform for IgY, by a selective extraction, was studied. The complete extraction and purification of IgY was not achieved in a single with the investigated ABS. However, the study of the proteins profile of the coexisting phases allowed to conclude that all proteins migrate for the top phase during the partitioning process, and that a complex between the ILs and the different proteins present in the water-soluble fraction of proteins from egg yolk was formed. Still, the results obtained reveal that the system composed of 20.1 wt % [N4444][CHES] + 16.9 wt % C6H5K3O7 is the most promising ABS for the IgY extraction.
Recentemente, a utilização de anticorpos para imunoterapia passiva tornou-se uma área de investigação atrativa. Tal facto deve-se à diminuição da eficiência dos antibióticos pelo aumento do número de organismos resistentes. Os anticorpos obtidos a partir da gema do ovo de galinhas imunizadas, imunoglobulinas Y (IgY), são uma alternativa viável e promissora aos anticorpos de mamíferos. Estes apresentam inúmeras vantagens, dado que podem ser obtidos em maior quantidade e serem recolhidos por técnicas menos invasivas. Apesar das vantagens apresentadas, as técnicas disponíveis para a extração e purificação de IgY são demoradas, trabalhosas, apresentam baixo rendimento, levam a baixos fatores de pureza, apresentam custos elevados e não são facilmente aplicáveis a nível industrial. Assim, o principal objetivo deste trabalho consistiu no desenvolvimento de uma plataforma de purificação alternativa para a extração e purificação de IgY, utilizando sistemas aquosos bifásicos (SAB) constituídos por líquidos iónicos com capacidade tampão. Neste trabalho foram estudados SAB constituídos por C6H5K3O7 e líquidos iónicos, sendo os últimos sintetizados pela combinação de aniões com capacidade tampão (Good’s buffers), com os catiões tetrabutilamónio ([N4444]+) e tetrabutilfosfónio ([P4444]+). Os líquidos iónicos utilizados permitiram o estudo da influência do anião e do catião nos diagramas de fase, ou seja sob a capacidade dos diferentes líquidos iónicos para formar SAB. De seguida, foi testada a capacidade destes sistemas como uma plataforma alternativa para a extração seletiva, e posterior purificação, de IgY. A purificação de IgY não foi conseguida num único passo de extração com os sistemas estudados. No entanto, o estudo do perfil proteico de ambas as fases dos sistemas permitiu concluir que, durante a partição, todas as proteínas migraram para a fase superior e que houve formação de um complexo entre o IL e algumas das proteínas constituintes da fração aquosa proteica da gema do ovo. Os resultados obtidos revelaram que, entre os vários SAB estudados, o mais promissor para a extração de IgY, é aquele constituído por 20.1 (m/m) % [N4444][CHES] + 16.9 (m/m) % C6H5K3O7.
Gilbert, Christopher Donald. "Non-Newtonian conversion of emulsion liquid membranes in the extraction of lead and zinc from simulated wastewater". Diss., Georgia Institute of Technology, 1999. http://hdl.handle.net/1853/10911.
Testo completoElias, Moacyr Jorge. "Determinação das condições de atividade otima, da estabilidade termica e da cinetica da hidrolise enzimatica de bromelina presente na casca e no talo do abacaxi (Ananas comosus L. Merril) variedade perola". [s.n.], 2010. http://repositorio.unicamp.br/jspui/handle/REPOSIP/267036.
Testo completoTese (doutorado) - Universidade Estadual de Campinas, Faculdade de Engenharia Quimica
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Resumo: A bromelina, enzima presente no abacaxi, hidrolisa ligações peptídicas das proteínas; tem aplicação em diversas áreas envolvendo alimentos, medicina e nutrição animal. No abacaxi a bromelina está presente no talo, na polpa e na casca do fruto. Visando avaliar a bromelina presente no fruto brasileiro Ananás comosus L. Merril, variedade pérola, enfocando seu aproveitamento quando recuperada a partir dos resíduos da industrialização, foram pesquisadas, em comparação com a bromelina pura, condições de pH e temperatura para maior atividade, estabilidade térmica ao longo do tempo em várias temperaturas e a cinética da sua atividade catalítica, empregando caseína como substrato. O extrato foi obtido pela trituração da casca e do talo interno do fruto e a reação de hidrólise, com pH controlado, efetuada em reator com 75 mL de volume útil sob constante agitação. Após a reação foi retirada amostra, adicionada em tubo contendo ácido tricloroacético e centrifugado, analisando a absorbância do sobrenadante. Atividade e a cinética foram expressas em mmol tirosina / L.minuto pela absorbância a 280 nm dos aminoácidos aromáticos gerados na hidrólise da caseína. Foram empregadas três relações enzima / substrato (em massa): 1 / 25, 1 / 50 e 1 / 125 para os ensaios relativos ao planejamento experimental em estrela tendo como ponto central pH em 7,0 e temperatura de 35 °C, os resultados foram tratados fornecendo as equações do modelo e as superfícies de resposta; as equações foram tratadas matematicamente fornecendo gráficos da melhor atividade em função da temperatura; os resultados do planejamento experimental mostraram similaridade entre a bromelina dos resíduos do fruto e a bromelina pura tomada como padrão. Para a estabilidade térmica os ensaios foram efetuados determinando a atividade para a relação 1 / 25 sob temperaturas variando entre 25 °C e 62 °C ao longo de 180 minutos com duas faixas de pH: a de melhor atividade definida no planejamento experimental (5,5 a 6,5) e entre 3,3 e 3,5; os resultados foram tratados analisando o gráfico da atividade em função do tempo mostrando que o modelo de ordem um é adequado para descrever a inativação térmica da enzima e que ela ocorre de maneira mais acentuada na faixa de pH 3,3 a 3,5 com valor do fator de freqüência k0 (minuto-1) 2,5 x1032 vezes maior para o extrato. Os ensaios para determinar a cinética da atividade catalítica da bromelina sobre a caseína foram efetuados a temperatura constante de 35 °C e pH de máxima atividade definido no planejamento experimental para cada uma das três relações enzima / substrato estudadas; foram elaboradas as curvas da concentração de aminoácidos formados ao longo do tempo (zero a 15 minutos) e os resultados tratados calculando a derivada das curvas no tempo zero (velocidade inicial); o modelo de Michaelis - Menten mostrou ser adequado para descrever o mecanismo de hidrólise da caseína pela bromelina e os resultados indicam que os valores da velocidade máxima (Vmax) e da constante de Michaelis (Km) são maiores para o extrato dos resíduos do fruto do que para a bromelina pura.
Abstract: The bromelain, enzyme found in pineapple, hydrolyses peptide protein bonds; there is application in several areas involving food, medicine and animal nutrition. In pineapple the bromelain is present in the flesh, skin and core of the fruit. Aiming to evaluate the bromelain present in Brazilian fruit Ananas comosus L. Merril, pérola type, focusing its use from industrialization residues recovering, it was searched, comparing with pure bromelain, pH conditions and temperature for higher activity, thermal stability along the time under several temperatures and the kinetics of its catalytic activity, using casein as a substrate. The extract was obtained crushing the skin and core of the fruit and the hydrolysis reaction, with controlled pH, done in a 75 mL net volume reactor under constant stirring. After reaction a sample was taken, added to a tube with tri chloroacetic acid and centrifuged, analyzing the supernatant absorbance. Activity and kinetics were expressed as mmol of tyrosine / L.min from absorbance at 280 nm of aromatic amino acids generated by casein hydrolysis. Three enzyme / substrate ratio were employed (weight basis):1 / 25, 1 / 50 and 1 / 125 for star type experimental design assays with central point at 7.0 for pH and at 35 °C for temperature, the results were processed giving the model equation and surface responses, the equations were mathematically treated giving graphics of best activity as a function of temperature; the experimental design results showed similarity between bromelain from fruit residues and pure bromelain taken as reference. The assays for thermal stability were carried out by activity determination for 1 / 25 ratio under temperatures varying from 25 °C and 62 °C during 180 minutes for two pH ranges: that for best activity defined from experimental design (5.5 to 6.5) and between 3.3 to 3.5; the results were treated by the analysis of the graphics of activity as a function of time showing that the order one model is adequate to describe the enzyme thermal inactivation and that it is stronger at pH range of 3.3 to 3.5 with values of frequency factor k0 (minute-1) 2.5 x1032 times bigger for the extract.The assays to determine the kinetics of bromelain catalytic activity on casein were carried out at constant temperature of 35 °C and pH of maximum activity defined by experimental design for each one of the three studied enzyme / substrate ratio; curves were built for the concentration of formed amino acids along the time (from zero to 15 minutes) and the results treated calculating the curves derivative at time zero (initial rate); the Michaelis - Menten model showed to be adequate to describe the casein hydrolysis mechanism of casein by bromelain and the results indicate that the maximum reaction rate (Vmax) values and the Michaelis constant (Km) values are bigger for the fruit residues extract than that for pure bromelain.
Doutorado
Sistemas de Processos Quimicos e Informatica
Doutor em Engenharia Química
Barros, Kleber Vanio Gomes. "Caracterização e purificação da enzima bromelina derivada do curaua (Ananas erectifolius) em sistema bifasico aquoso PEG/fosfato". [s.n.], 2009. http://repositorio.unicamp.br/jspui/handle/REPOSIP/267111.
Testo completoDissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Engenharia Quimica
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Resumo: O curauá (Ananas erectifolius) é uma planta fibrosa proveniente da Amazônia brasileira, pertencente à família Bromeliaceae e ao gênero Ananas. A bromelina é uma protease de origem vegetal, obtida de diversas espécies da família Bromeliaceae. A bromelina tem utilização bastante difundida em processos industriais nas áreas alimentícia e farmacêutica. Efeitos antiinflamatórios da enzima, como também, o seu uso no tratamento da angina, na indigestão entre outros, estão documentados na literatura científica. Estudos de métodos para purificação de biomoléculas que viabilizem a sua obtenção em larga escala com custo reduzido são importantes áreas de pesquisa na biotecnologia. A extração líquido-líquido através de sistemas bifásicos aquosos (SBA) pode ser usada como técnica de pré-purificação de bioprodutos. Estes sistemas formam duas fases aquosas imiscíveis ou parcialmente miscíveis entre si. Podem ser formados a partir da mistura de dois polímeros ou de um polímero e um sal, a exemplo do sistema PEG (polietilenoglicol)/fosfato de potássio. Foi estudada a enzima bromelina derivada das folhas de ambas as variedades: curauá branco e curauá roxo. Pesquisou-se também a recuperação da enzima por extração líquido-líquido em sistemas de duas fases aquosas PEG/fosfato. Realizou-se ensaios em batelada para a extração e recuperação da enzima, utilizando como indicador o coeficiente de partição. Obteve-se fatores de purificação da enzima bromelina para o sistema fosfato de potássio e polietileno glicol 4000 e 6000, nos pH de 7,0; 8,0 e 9,0 a 25 °C. Estudou-se a partição em três diferentes "tie-lines". Analisou-se a influência do pH e do comprimento das linhas de amarração no coeficiente de partição da enzima. Na purificação da bromelina derivada das folhas do curauá branco, observou-se os melhores resultados no sistema bifásico aquoso PEG 4000/Fosfato em pH 7,0. Observou-se que o sistema PEG 6000/Fosfato em pH 7,0 apresentou os melhores resultados em relação à purificação da enzima bromelina derivada das folhas do curauá roxo.
Abstract: The curauá (Ananas erectifolius) is a fibrous plant from brazilian Amazon, belonging to the Bromeliaceae family and Ananas genus. Bromelain is a protease from vegetable origin, obtained from several species of this family. The bromelain has widespread use in industries process, such as in food and pharmaceutical areas. Antiinflammatory effects enzyme and also it uses in angina treatment, indigestion among others are documented in scientific literature. Methods' studies to purification of biomolecules that allow their achievement in large scale with low cost are important research's areas in biotechnology. The liquid-liquid extraction through aqueous twophase system (ATPS) can be used as technical of pre-purification of bioproduct. These systems form aqueous two-phase immiscible or partially miscible within themselves. They can be obtained by the addition of two polymers or one polymer and a salt, such as the PEG - poly(ethyleneglycol) - and potassium phosphate salt system. In the present report we characterized the bromelain enzyme that comes from the leaves of white curauá and purple curauá, and also investigated the recuperation of the enzyme by liquid-liquid extraction in aqueous two-phases PEG-phosphate systems (ATPS). The assays to extraction and recovery of the enzyme were carried out in batch mode, using the partition coefficient as indicator. Getting to factors purification of the enzyme bromelin to PEG 4000 and 6000 and potassium phosphate salt in ATPS, at pH 7.0, 8.0 and 9.0 at 25 ºC. We studied the partition through three different tie-lines. The influences of pH and tie-line length in partition coefficient of enzyme were analyzed. In the purification of bromelain derived from the leaves of white curauá, we observed the best results in PEG 4000/phosphate ATPS at pH 7.0. Furthermore, the system PEG 6000/phosphate at pH 7.0 showed the best results in relation to the purification of the enzyme bromelain derived from the leaves of purple curauá.
Mestrado
Sistemas de Processos Quimicos e Informatica
Mestre em Engenharia Química
Libri sul tema "Liquid/liquid extraction purification"
Schoepfer, Matthieu. Evaluation of liquid-liquid extraction as a wastewater purification process. Manchester: UMIST, 1996.
Cerca il testo completoHiler, G. V. Liquid-liquid extraction of trace level pesticides from process streams. Research Triangle Park, NC: U.S. Environmental Protection Agency, Air and Energy Engineering Research Laboratory, 1985.
Cerca il testo completoHiler, G. V. Liquid-liquid extraction of trace level pesticides from process streams. Research Triangle Park, NC: U.S. Environmental Protection Agency, Air and Energy Engineering Research Laboratory, 1985.
Cerca il testo completoHiler, G. V. Liquid-liquid extraction of trace level pesticides from process streams. Research Triangle Park, NC: U.S. Environmental Protection Agency, Air and Energy Engineering Research Laboratory, 1985.
Cerca il testo completoHiler, G. V. Liquid-liquid extraction of trace level pesticides from process streams. Research Triangle Park, NC: U.S. Environmental Protection Agency, Air and Energy Engineering Research Laboratory, 1985.
Cerca il testo completoHiler, G. V. Liquid-liquid extraction of trace level pesticides from process streams. Research Triangle Park, NC: U.S. Environmental Protection Agency, Air and Energy Engineering Research Laboratory, 1985.
Cerca il testo completoBlumberg, Ruth. Liquid-liquid extraction. London: Academic Press, 1988.
Cerca il testo completoC, Godfrey J., e Slater M. J, a cura di. Liquid-liquid extraction equipment. Chichester: Wiley, 1994.
Cerca il testo completoC, McArthur, Institution of Chemical Engineers e Dounreay Nuclear Power Development Establishment., a cura di. Extraction '84: Symposium on Liquid-Liquid Extraction Science. Rugby: The Institution, 1985.
Cerca il testo completoNajim, K. Control of liquid-liquid extraction columns. New York: Gordon and Breach, 1988.
Cerca il testo completoCapitoli di libri sul tema "Liquid/liquid extraction purification"
Zhang, Jianguo, e Bo Hu. "Liquid-Liquid Extraction (LLE)". In Separation and Purification Technologies in Biorefineries, 61–78. Chichester, UK: John Wiley & Sons, Ltd, 2013. http://dx.doi.org/10.1002/9781118493441.ch3.
Testo completoKelley, Brian D., e T. Alan Hatton. "Protein Purification by Liquid-Liquid Extraction". In Biotechnology, 593–616. Weinheim, Germany: Wiley-VCH Verlag GmbH, 2008. http://dx.doi.org/10.1002/9783527620845.ch22.
Testo completoVahan Kilikian, Beatriz, Telma Teixeira Franco, Jane S. R. Coimbra, Antonio J. A. Meirelles, Adalberto Pessoa e Adamu Muhammad Alhaji. "Liquid–Liquid Extraction in Aqueous Two-Phase Systems". In Purification of Biotechnological Products, 155–80. Boca Raton: CRC Press, 2024. http://dx.doi.org/10.1201/9781032726823-7.
Testo completoAbidin, Zurina Zainal, Dayang Radiah Awang Biak, Hamdan Mohamed e Mohd Yusof Harun. "Solid-Liquid Extraction in Biorefinery". In Separation and Purification Technologies in Biorefineries, 351–74. Chichester, UK: John Wiley & Sons, Ltd, 2013. http://dx.doi.org/10.1002/9781118493441.ch13.
Testo completoCarneiro-Da-Cunha, M. G., E. P. Melo, M. J. Sebastião, M. R. Aires-Barros e J. M. S. Cabral. "Separation and Purification of Lipases by Liquid-Liquid Extraction Processes". In Engineering of/with Lipases, 551–76. Dordrecht: Springer Netherlands, 1996. http://dx.doi.org/10.1007/978-94-009-1671-5_35.
Testo completoSamar, Mohammed, Dominique Pareau, Gérard Durand e André Chesné. "Purification of waste waters containing heavy metals; by surfactant liquid membrane extraction". In Hydrometallurgy ’94, 635–54. Dordrecht: Springer Netherlands, 1994. http://dx.doi.org/10.1007/978-94-011-1214-7_42.
Testo completoGhasem, Nayef. "Liquid–Liquid Extraction". In Computer Methods in Chemical Engineering, 377–416. 2a ed. Boca Raton: CRC Press, 2021. http://dx.doi.org/10.1201/9781003167365-8.
Testo completoBart, Hans-Jörg. "Liquid-Liquid Phase Equilibria". In Reactive Extraction, 17–50. Berlin, Heidelberg: Springer Berlin Heidelberg, 2001. http://dx.doi.org/10.1007/978-3-662-04403-2_2.
Testo completoSchügerl, Karl. "Liquid-Liquid Extraction (Small Molecules)". In Biotechnology, 557–92. Weinheim, Germany: Wiley-VCH Verlag GmbH, 2008. http://dx.doi.org/10.1002/9783527620845.ch21.
Testo completoRupasinghe, Thusitha. "Liquid Extraction: Acidic Extraction". In Encyclopedia of Lipidomics, 1–2. Dordrecht: Springer Netherlands, 2016. http://dx.doi.org/10.1007/978-94-007-7864-1_93-1.
Testo completoAtti di convegni sul tema "Liquid/liquid extraction purification"
Liu, Longyun, Jin Liang, Rui Huang e Yuyue Zhao. "Design of scale graphite automatic purification device based on liquid surface discharge plasma technology". In 4th International Conference on Precision Instruments and Optical Engineering (PIOE 2024), a cura di Zhengji Xu e Jianwei Wu, 41. SPIE, 2024. http://dx.doi.org/10.1117/12.3054878.
Testo completoHe, Fei, e Qiang Wan. "Study on Purification of U Scraps in Continuous Countercurrent Extractor". In 2017 25th International Conference on Nuclear Engineering. American Society of Mechanical Engineers, 2017. http://dx.doi.org/10.1115/icone25-66336.
Testo completoAbdurrosyid, Imam, Fajar Muhammad Ramadhan, Muhammad Afton Muhandis, Intan Nurhimawati, Muhammad Ilham Bayquni, Anas Fahmi Imron, Nuradam Effendy, Nadia Tina Aisyah e Zeni Ulma. "The effect of HNO3 molarity and TBP concentration to the purification of rare earth metals by liquid-liquid extraction method using TBP:Kerosene". In INTERNATIONAL CONFERENCE ON NUCLEAR SCIENCE, TECHNOLOGY, AND APPLICATIONS – ICONSTA 2022. AIP Publishing, 2024. http://dx.doi.org/10.1063/5.0194127.
Testo completoJingru, Shen, e Xu Yong. "Separation and Purification of Bovine Serum Albumin by Non-Organic Solvent Liquid-Solid Extraction System". In 2007 1st International Conference on Bioinformatics and Biomedical Engineering. IEEE, 2007. http://dx.doi.org/10.1109/icbbe.2007.22.
Testo completoMao, Xiaole, Sung Yang e Jeffrey D. Zahn. "Experimental Demonstration and Numerical Simulation of Organic-Aqueous Liquid Extraction Enhanced by Droplet Formation in a Microfluidic Channel". In ASME 2006 International Mechanical Engineering Congress and Exposition. ASMEDC, 2006. http://dx.doi.org/10.1115/imece2006-16084.
Testo completoMorales, Mercedes C., e Jeffrey D. Zahn. "Development of a Diffusion Limited Microfluidic Module for DNA Purification via Phenol Extraction". In ASME 2008 International Mechanical Engineering Congress and Exposition. ASMEDC, 2008. http://dx.doi.org/10.1115/imece2008-68086.
Testo completoIlin, V., Yu Karlin, A. Laurson, Eu Volkov e S. Dmitriev. "Possible Approach to Cleaning “Problematic” LRW With Large Contents of Suspended Particles, Oils and Other Organic Substances". In The 11th International Conference on Environmental Remediation and Radioactive Waste Management. ASMEDC, 2007. http://dx.doi.org/10.1115/icem2007-7146.
Testo completoKishimoto, Y. "Liquid Scintillator Purification". In TOPICAL WORKSHOP ON LOW RADIOACTIVITY TECHNIQUES: LRT 2004. AIP, 2005. http://dx.doi.org/10.1063/1.2060471.
Testo completoBousbaa, Hamza, Khatir NAIMA, Medjahed Lamia, Mohammed Benramdane, Dhinesh Balasubramanian e Anish Jafrin Thilak Johnson. "Physicochemical Characterization and Potential Applications of Biodiesel Produced from Industrial Fish Waste". In Automotive Technical Papers. 400 Commonwealth Drive, Warrendale, PA, United States: SAE International, 2024. http://dx.doi.org/10.4271/2024-01-5106.
Testo completoIkeda, Haruo. "Purification of KamLAND-Zen liquid scintillator". In LOW RADIOACTIVITY TECHNIQUES 2013 (LRT 2013): Proceedings of the IV International Workshop in Low Radioactivity Techniques. AIP, 2013. http://dx.doi.org/10.1063/1.4818107.
Testo completoRapporti di organizzazioni sul tema "Liquid/liquid extraction purification"
Majumdar, S., A. K. Guha, Y. T. Lee, T. Papadopoulos e S. Khare. Liquid membrane purification of biogas. Office of Scientific and Technical Information (OSTI), marzo 1991. http://dx.doi.org/10.2172/5065322.
Testo completoPier, Rose Angeli C., e Rebecca M. Chamberlin. Liquid-Liquid Extraction of Iron in Hydrochloric Acid with Quaternary Amines in Microfluidic Devices. Office of Scientific and Technical Information (OSTI), agosto 2018. http://dx.doi.org/10.2172/1467377.
Testo completoCarleson, T. E. The effect of electric fields upon liquid extraction. Office of Scientific and Technical Information (OSTI), aprile 1988. http://dx.doi.org/10.2172/6977782.
Testo completoDelegard, Calvin H., e Amanda J. Casella. Literature Review: Crud Formation at the Liquid/Liquid Interface of TBP-Based Solvent-Extraction Processes. Office of Scientific and Technical Information (OSTI), settembre 2016. http://dx.doi.org/10.2172/1430463.
Testo completoGasbarro, Christina. Microfluidic Liquid-Liquid Extraction Chip with Integrated Raman Sensors Phase I and Phase II Final Report. Office of Scientific and Technical Information (OSTI), agosto 2021. http://dx.doi.org/10.2172/1812310.
Testo completoGriffith, W. L., A. L. Compere, J. M. Googin e W. P. Huxtable. Aluminum nitrate recrystallization and recovery from liquid extraction raffinates. Office of Scientific and Technical Information (OSTI), settembre 1991. http://dx.doi.org/10.2172/6026818.
Testo completoXu, Yanchen. Liquid metal extraction of Nd from NdFeB magnet scrap. Office of Scientific and Technical Information (OSTI), dicembre 1999. http://dx.doi.org/10.2172/754780.
Testo completoChow, L. S., J. K. Basco, J. P. Ackerman e T. R. Johnson. Continuous extraction of molten chloride salts with liquid cadmium alloys. Office of Scientific and Technical Information (OSTI), settembre 1993. http://dx.doi.org/10.2172/10180823.
Testo completoCarleson, T. E. The effect of electric fields upon liquid extraction. Progress report. Office of Scientific and Technical Information (OSTI), aprile 1988. http://dx.doi.org/10.2172/10182284.
Testo completoFarawila, Anne F., Matthew J. O'Hara, Chien M. Wai, Harry Z. Taylor e Yu-Jung Liao. Selective Extraction of Uranium from Liquid or Supercritical Carbon Dioxide. Office of Scientific and Technical Information (OSTI), luglio 2012. http://dx.doi.org/10.2172/1062508.
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