Letteratura scientifica selezionata sul tema "Integrative conjugative element (ICE)"
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Articoli di riviste sul tema "Integrative conjugative element (ICE)":
Iannelli, Francesco, Francesco Santoro, Marco R. Oggioni e Gianni Pozzi. "Nucleotide Sequence Analysis of Integrative Conjugative Element Tn5253of Streptococcus pneumoniae". Antimicrobial Agents and Chemotherapy 58, n. 2 (2 dicembre 2013): 1235–39. http://dx.doi.org/10.1128/aac.01764-13.
Libante, Virginie, Nazim Sarica, Abbas Mohamad Ali, Chloé Gapp, Anissa Oussalah, Gérard Guédon, Nathalie Leblond-Bourget e Sophie Payot. "Mobilization of IMEs Integrated in the oriT of ICEs Involves Their Own Relaxase Belonging to the Rep-Trans Family of Proteins". Genes 11, n. 9 (26 agosto 2020): 1004. http://dx.doi.org/10.3390/genes11091004.
Del Grosso, Maria, Romina Camilli, Ermanno Rizzi, Alessandro Pietrelli, Gianluca De Bellis e Annalisa Pantosti. "ICESpy009, a Conjugative Genetic Element Carryingmef(E) in Streptococcus pyogenes". Antimicrobial Agents and Chemotherapy 60, n. 7 (11 aprile 2016): 3906–12. http://dx.doi.org/10.1128/aac.03082-15.
Giovanetti, Eleonora, Andrea Brenciani, Erika Tiberi, Alessandro Bacciaglia e Pietro Emanuele Varaldo. "ICESp2905, theerm(TR)-tet(O) Element of Streptococcus pyogenes, Is Formed by Two Independent Integrative and Conjugative Elements". Antimicrobial Agents and Chemotherapy 56, n. 1 (10 ottobre 2011): 591–94. http://dx.doi.org/10.1128/aac.05352-11.
Michael, G. B., K. Kadlec, M. T. Sweeney, E. Brzuszkiewicz, H. Liesegang, R. Daniel, R. W. Murray, J. L. Watts e S. Schwarz. "ICEPmu1, an integrative conjugative element (ICE) of Pasteurella multocida: structure and transfer". Journal of Antimicrobial Chemotherapy 67, n. 1 (14 ottobre 2011): 91–100. http://dx.doi.org/10.1093/jac/dkr411.
Auchtung, Jennifer M., Naira Aleksanyan, Artemisa Bulku e Melanie B. Berkmen. "Biology of ICE Bs1 , an integrative and conjugative element in Bacillus subtilis". Plasmid 86 (luglio 2016): 14–25. http://dx.doi.org/10.1016/j.plasmid.2016.07.001.
Calcutt, Michael J., Michelle S. Lewis e Kim S. Wise. "Molecular Genetic Analysis of ICEF, an Integrative Conjugal Element That Is Present as a Repetitive Sequence in the Chromosome of Mycoplasma fermentans PG18". Journal of Bacteriology 184, n. 24 (15 dicembre 2002): 6929–41. http://dx.doi.org/10.1128/jb.184.24.6929-6941.2002.
McKeithen-Mead, Saria A., e Alan D. Grossman. "Timing of integration into the chromosome is critical for the fitness of an integrative and conjugative element and its bacterial host". PLOS Genetics 19, n. 2 (13 febbraio 2023): e1010524. http://dx.doi.org/10.1371/journal.pgen.1010524.
Levicán, Gloria J., Assaf Katz, Jorge H. Valdés, Raquel Quatrini, David S. Holmes e O. Orellana. "A 300 kpb Genome Segment, Including a Complete Set of tRNA Genes, is Dispensable for Acidithiobacillus Ferrooxidans". Advanced Materials Research 71-73 (maggio 2009): 187–90. http://dx.doi.org/10.4028/www.scientific.net/amr.71-73.187.
Aberkane, Salim, Fabrice Compain, Dominique Decré, Chloé Dupont, Chrislène Laurens, Marion Vittecoq, Alix Pantel et al. "High Prevalence of SXT/R391-Related Integrative and Conjugative Elements CarryingblaCMY-2in Proteus mirabilis Isolates from Gulls in the South of France". Antimicrobial Agents and Chemotherapy 60, n. 2 (7 dicembre 2015): 1148–52. http://dx.doi.org/10.1128/aac.01654-15.
Tesi sul tema "Integrative conjugative element (ICE)":
Coluzzi, Charles. "L'exploration des génomes par l'outil ICEFinder révèle la forte prévalence et l'extrême diversité des ICE et des IME de streptocoques". Thesis, Université de Lorraine, 2017. http://www.theses.fr/2017LORR0352/document.
Mobile genetic elements largely contribute to the evolution and diversity of bacterial genomes through horizontal gene transfer. Among them, the integrative and conjugative elements (ICEs) encode their own excision, conjugative transfer and integration. On the other hand, integrative mobilizable elements (IMEs) are autonomous for excision and integration but encode only some of the proteins needed for their conjugative transfer. IMEs therefore need a “helper” conjugative element to transfer. Despite their impact on gene flow and genome dynamics, the prevalence of ICEs remains largely underscored and very few IMEs were identified at the beginning of this study. Furthermore, although several in silico methods exist to detect genomic islands, none are dedicated to ICEs or IMEs, thus complicating exhaustive examination of these mobile elements. The Streptococcus genus belongs to the firmicutes’ phylum. Almost all streptococci are commensal bacteria or pathogenes to men and animals. Two species of Streptococcus are also used in the dairy industry as lactic ferments in order to produce fermented milk and different types of cheese. Studying the gene flux of the Steptococci genus and the impact it can have on the lifestyle of these organisms is essential, as it has a lot of interest for human health and activities. In this work, we searched for ICEs and IMEs in 124 strains of streptococci belonging to 27 species using a reference database of ICE and IME signature proteins (from their conjugation, mobilization and integration/excision modules). This exhaustive analysis led to the identification and delimitation of 131 ICEs or slightly decayed ICEs and 144 IMEs. All these elements were delimited, which allowed us to identify their integration specificities in the genomes. In total, 17 ICE integration specificities were identified. Among them, 8 had never been described before (ftsK, guaA, lysS, mutT, rpmG, rpsI, traG and ybaB/EbfC). 18 specificities were also identified for IMEs, among which only 5 were known for the firmicutes. ICEs encode high or low-specificity tyrosine integrases (13 different specificities), single serine intégrases (1 specificity), triplet of serine integrases (3 different specificities), or DDE transposases while IMEs encode either tyrosine integrases (10 different specificities) or single serine integrases (8 different specificities). ICE were grouped in 7 distinct families according to the proteins encoded by their conjugation module whereas the mobilization modules of IMEs were highly diverse, preventing them from grouping into families according to their mobilization modules. The phylogenetic analysis of the signature proteins encoded by all ICEs and IMEs showed integration module exchanges between ICEs and IMEs and several mobilization module exchanges between IMEs. The overall results reveal a strong prevalence and extreme diversity of these elements among Streptococci genomes. Better understanding and knowledge of ICEs and IMEs prompted us to build a semi-automated command-line tool to identify streptococcal ICEs and IMEs as well as to determine their insertion site
Lao, Julie. "Conception et mise en oeuvre d’une approche bioinformatique dédiée à l’identification des ICE, IME et éléments composites dans les génomes de Firmicutes". Electronic Thesis or Diss., Université de Lorraine, 2021. http://www.theses.fr/2021LORR0063.
ICEs (Integrative Conjugative Elements) and IMEs (Integrative Mobilizable Elements) are bacterial mobile elements that play a key role in horizontal transfers. They have the capacity to integrate and transfer by conjugation from one bacterium to another. These elements are widespread in bacterial genomes but are still poorly understood. Their automatic identification is a challenge and they are generally not annotated in genomes. So far, only two bioinformatic approaches allow the detection of ICEs and IMEs, but their reliability remains highly variable, particularly among Firmicutes. Moreover, neither of these approaches can accurately detect composite elements consisting of nested or accreted ICEs and IMEs, which are frequently observed in bacterial genomes. We have developed a strategy and a tool called ICEscreen to identify ICEs and IMEs in the genomes of Firmicutes, including nested or accreted elements. Our tool starts with the detection of four signature proteins (SPs) that are essential to the functioning of these elements and then carries out the detection and typing of the elements based on the colocalization of the SPs and the characterisation of their content. Our tool uses a dedicated algorithm to solve the structure of the elements whether they are composite or not. To perform these steps, we have built a bank of ICEs and IMEs signature proteins from a list of genes known to be involved in the dynamics of these elements in streptococci and also public HMM profiles and HMM profiles constructed especially for this study. To validate the ICEscreen results, we built a dataset, FirmiData, consisting of 40 genomes of Firmicutes for which the ICEs and IMEs were annotated semi-manually and curated. We then compared the results of ICEscreen with those of two reference tools: CONJscan and ICEfinder. ICEscreen detects almost all the elements of the reference (96%) making it a more powerful tool than CONJscan (58%) and especially ICEfinder (53%) on our dataset. ICEscreen is thus a tool for the annotation and discovery of ICE and IME in the genomes of Firmicutes, which can help to better characterize their contribution to horizontal gene transfers, particularly during the transmission of antibiotic resistance, with which they are frequently associated
Laroussi, Haifa. "Étude des mécanismes moléculaires d'initiation du transfert conjugatif d'ICESt3, médiée par une relaxase MOBT chez la bactérie Gram+ Streptococcus thermophilus". Electronic Thesis or Diss., Université de Lorraine, 2022. http://www.theses.fr/2022LORR0176.
Bacterial genomes evolve mainly through horizontal gene transfer. Bacterial conjugation is one of the major mechanisms for these transfers. Conjugation is mediated by integrative and conjugative elements (ICE). In addition to their transfer function, ICEs encode other functions that may provide an adaptive advantage to their host, such as resistance to antibiotics whose dissemination is a major public health issue. It is therefore necessary to understand how ICEs are transferred in order to limit their dissemination.The transfer of an ICE from a donor cell to a recipient cell requires its excision from the chromosome, its transfer from one cell to the other and then its integration into the genomes of the two partner cells. According to the literature, the initiation of ICE transfer is mediated by a nucleoprotein complex called relaxosome, whose key protein is the relaxase, a transesterase encoded by the element. The role of the relaxase is to perform a single-stranded cleavage on the DNA of the ICE at a conserved site, called nic. This cleavage releases a free 3'OH end, used as a primer to initiate rolling circle replication. The DNA-relaxase complex is then driven to the conjugation pore.During my PhD thesis, I studied ICESt3 from Streptococcus thermophilus which belongs to the ICESt3/Tn916/ICEBs1 superfamily, widespread among Firmicutes. These ICEs encode a non-canonical relaxase belonging to the MOBT family, which is related to the rolling circle replication initiators of the Rep_trans family. The general objective of my thesis was to elucidate the function of the RelSt3 relaxase in order to decipher the molecular mechanisms of initiation of conjugative transfer mediated by a MOBT relaxase.My work led to the identification of the RelSt3 binding site on ICESt3 origin of transfer (oriT). This site, called bind, is peculiar in that it is distant from the nic site, which is not the case for other relaxase families. RelSt3 possesses an HTH domain at its N-terminus. I have shown that this domain is required for the binding of RelSt3 to its bind site, and that it is important for its catalytic activity. Conjugation assays demonstrated that this HTH domain is crucial for the conjugative transfer of ICESt3. Structural predictions of the HTH domain in complex with DNA led to the identification of the interaction interface with the bind site, confirmed by mutagenesis. I also demonstrated that RelSt3 exhibits a nicking-closing activity and that it covalently binds to the 5' end of the cleaved strand, demonstrating that this enzyme participates in both initial and final steps of conjugation.In the literature, it has been shown that relaxases interact frequently with other accessory proteins, encoded by the ICE or by the host bacteria, participating in relaxosome formation. The second objective of my thesis was to identify RelSt3 partners. Comparisons with available data on ICEBs1 from Bacillus subtilis allowed to identify two candidate proteins, OrfL and OrfM, that may belong to the relaxosome of ICESt3, as well as a cellular helicase, PcrA , probably involved in the rolling circle replication. A characterization of these proteins was performed using biochemical and biophysical approaches. The interaction network between all of these proteins was established using in vitro approaches, as well as with the in vivo two-hybrid approach. These data provide a first insight into the components of the ICESt3 relaxasome. I also showed that OrfL and OrfM stimulate the catalytic activity of RelSt3 in vitro, and that they are both essential for ICESt3 conjugation.This work lead to a better understanding of the molecular mechanisms required during the conjugation of an ICE driven by a MOBT family relaxase
Nouvel, Laurent-Xavier. "Etude de la diversité génétique de Mycoplasma agalactiae : plasticité des génomes, mobilome et dynamique de surface". Thesis, Toulouse, INPT, 2009. http://www.theses.fr/2009INPT013A/document.
Mycoplasma agalactiae is responsible of contagious agalactia, a disease of small ruminants that is still difficult to control and is listed by the OIE. In order to evaluate the genetic diversity of this pathogen, 101 isolates were compared using three techniques (VNTR, RFLP, vpma repertoire). Results revealed a high genetic homogeneity with the PG2 type strain as representative. Some isolates however diverged such as the 5632 which was sequenced and analysed here. Whole comparative genomic and proteomic analyses of the 5632 and PG2 strains indicate that their genomic plasticity resides in important genes flux and in the presence of several mobile genetic elements (10% of the genome). These analyses also revealed that specific loci encoding repertoire of surface proteins are highly dynamic. For these minimal bacteria that lack a cell-wall, these events have most likely played a major role in their survival and adaptation to complex hosts
SANTORO, FRANCESCO. "Functional characterization of the pneumococcal Integrative Conjugative Element Tn5253". Doctoral thesis, Università di Siena, 2017. http://hdl.handle.net/11365/1005925.
Harden, Mark Michael Jr. "Interactions between an integrative and conjugative element and its bacterial host". Thesis, Massachusetts Institute of Technology, 2021. https://hdl.handle.net/1721.1/130662.
Cataloged from the official PDF of thesis.
Includes bibliographical references.
Conjugative elements are mobile genetic elements that can transfer from a donor bacterium to a recipient via an element-encoded type IV secretion system. Integrative and conjugative elements (ICEs) are an abundant class of conjugative element. ICEs are typically integrated into the bacterial host chromosome, but under certain conditions, or stochastically, they can excise from the chromosome and transfer to a recipient. ICEs likely interact with their bacterial host at every stage of their life cycle, but few of these interactions have been characterized. In this work I sought to 1) identify bacterial host factors necessary for efficient transfer of the integrative and conjugative element ICEBs1 to a recipient, and 2) determine whether the ICEBs1-encoded cell wall-modifying enzyme CwlT acts on the cell wall of the donor bacterium, the recipient bacterium, or both.
I used CRISPR interference to induce a knockdown of individual essential Bacillus subtilis genes, and then screened for gene knockdowns that caused an acute defect in transfer of ICEBs1. I found that wall teichoic acids were necessary in both ICEBs1 donors and recipients for efficient conjugative transfer. I found that depletion of wall teichoic acids caused cells involved in ICEBs1 conjugation to sustain lethal envelope damage caused by active conjugation machinery. Conjugative elements must bypass the cell wall of both the donor and recipient cells in a mating pair. Conjugative elements encode cell wall hydrolases that are required for efficient transfer, which are presumed to partly degrade the cell wall of the donor bacterium during conjugation. In order to investigate the role of the ICEBs1-encoded cell wall hydrolase CwlT in conjugation, I generated cell wall-less (L-form) strains of B. subtilis which could donate or receive ICEBs1.
In the absence of either the donor or recipient cell wall, CwlT was dispensable for efficient transfer. This finding indicates that CwlT acts on both the donor and recipient cell wall in a mating pair.
by Mark Michael Harden, Jr.
Ph. D.
Ph.D. Massachusetts Institute of Technology, Department of Biology
Avello, Monika M. M. (Monika Maria Masumi). "Characterization of an exclusion mechanism in an integrative and conjugative element in Bacillus subtilis". Thesis, Massachusetts Institute of Technology, 2018. http://hdl.handle.net/1721.1/119980.
Cataloged from PDF version of thesis.
Includes bibliographical references.
Horizontal gene transfer is the acquisition of new genetic material that can confer novel phenotypes to bacteria and contribute to their evolution. Conjugation is an important mechanism of horizontal gene transfer that involves the direct transfer of DNA between two cells and is mediated by mobile genetic elements encoding type IV secretion systems. Conjugative elements prevent redundant transfer by a mechanism known as exclusion that inhibits their cognate secretion systems. Exclusion is prevalent among conjugative elements, suggesting it is advantageous and potentially essential. Yet very few exclusion mechanisms are characterized, and the advantages they provide are not well understood. My work characterizes the exclusion mechanism of an integrative and conjugative element found in a Gram-positive bacterium. In combination with several other studies, my results point to a potentially conserved mechanism and novel benefits of this phenomenon, furthering our understanding of how mobile genetic elements regulate their transfer, impact their bacterial hosts, and mediate horizontal gene transfer.
by Monika M. M. Avello.
Ph. D.
Eidam, Christopher [Verfasser]. "Molecular analysis of multiresistant Mannheimia haemolytica isolates with particular reference to novel macrolide resistance genes and variants of the integrative and conjugative element ICEPmu1 / Christopher Eidam". Hannover : Bibliothek der Tierärztlichen Hochschule Hannover, 2014. http://d-nb.info/106486256X/34.
fox, Valeria. "Mobile genetic elements carrying stress response systems, antibiotic resistance determinants, and catabolic pathways". Doctoral thesis, Università di Siena, 2021. http://hdl.handle.net/11365/1159250.
Carraro, Nicolas. "Analyse comparative de la dynamique de deux éléments intégratifs conjugatifs de streptococcus thermophilus". Thesis, Nancy 1, 2011. http://www.theses.fr/2011NAN10080/document.
Integrative and Conjugative Elements (ICEs) are genomic islands, which excise from the chromosome, self-transfer by conjugation and integrate. They harbor a modular organization: genes and sequences involved in the same biological process are grouped in the same region. This work concerns the modality of transfer and maintenance of ICESt1 and ICESt3, two ICEs of Streptococcus thermophilus that share closely related core region. ICESt1 excises much less frequently than ICESt3. Nevertheless, excision of the two elements is activated by the same stimuli (DNA damage, stationary phase and/or cell density) and depends of the host strain. Bioinformatical and transcriptional analyses highlight several differences in their organization. However, each of these two ICEs would encode two different regulators, cI and ImmR, suggesting that a complex and original pathway govern to ICESt1' and ICESt3' regulation. This regulation would be shared with numerous ICEs that we identified in the genome of various commensal or pathogenic streptococci. According to the original definition, ICE's maintenance would be exclusively due to their integration in the host chromosome, and ICEs would not be able of extracellular replication. However, in addition to the induction of ICESt3' excision and transfer, DNA damage cause replication of its extrachromosomal form. This unexpected property is encoded by the core region and would be implicated in the maintenance of the element. Comparision with data recently published on other ICEs suggest that intracellular replication could be involved in the maintenance of numerous ICEs, besides their integration
Capitoli di libri sul tema "Integrative conjugative element (ICE)":
Armshaw, Patricia, e J. Tony Pembroke. "UV Stress-Responsive Genes Associated with Enterobacterial Integrative Conjugative Elements of the ICE SXT/R391 Group". In Stress and Environmental Regulation of Gene Expression and Adaptation in Bacteria, 517–27. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2016. http://dx.doi.org/10.1002/9781119004813.ch48.
Berkmen, Melanie B., Stephanie J. Laurer, Bridget K. Giarusso e Rodrigo Romero. "The Integrative and Conjugative Element ICEBs1 of Bacillus subtilis". In Bacterial Integrative Mobile Genetic Elements, 201–16. Boca Raton: CRC Press, 2022. http://dx.doi.org/10.1201/9780367813925-12.