Tesi sul tema "Hyperbaric oxygenation"
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Hammarlund, Christer. "Hyperbaric oxygenation and wound repair in man effects on the dermal microcirculation /". Helsingborg : Dept. of Anaesthesia, Helsingborg Hospital, 1995. http://books.google.com/books?id=sdxsAAAAMAAJ.
Testo completoKunin, Wendy. "Hyperbaric oxygen therapy following arthroscopic meniscectomy surgery". Thesis, McGill University, 2004. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=80308.
Testo completoAllie, Dean Gerard. "An assessment of the viability of establishing a hyperbaric oxygen therapy facility in the Nelson Mandela Metropolitan Municipality area". Thesis, Nelson Mandela Metropolitan University, 2005. http://hdl.handle.net/10948/151.
Testo completoMcGavock, Jonathan M. "The effect of hyperbaric oxygen therapy on aerobic performance following fatigue". Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape9/PQDD_0027/MQ50544.pdf.
Testo completoVudiniabola, Sunia. "Hyperbaric oxygen therapy for the treatment and prevention of osteoradionecrosis /". Title page, contents and summary only, 1997. http://web4.library.adelaide.edu.au/theses/09DM/09dmv986.pdf.
Testo completoBennett, Michael Heywood Prince of Wales Clinical School UNSW. "The evidence basis of diving and hyperbaric medicine - a synthesis of the high level clinical evidence with meta-analysis". Awarded by:University of New South Wales. Prince of Wales Clinical School, 2006. http://handle.unsw.edu.au/1959.4/24243.
Testo completoGermain, Geneviève. "Effect of hyperbaric oxygen therapy on exercise-induced muscle injury". Thesis, McGill University, 2002. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=29504.
Testo completoLiebich, Ingrid. "Hyperbaric oxygen therapy for children with cerebral palsy : Jebsen-Taylor test of hand function". Thesis, McGill University, 2001. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=31117.
Testo completoHodges, Alastair N. H. "Effect of hyperbaric oxygen on venous PO2, transcutaneous PO2, and VO2max in a normobaric environment". Thesis, McGill University, 2000. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=30175.
Testo completoSkelton, Deborah. "The effects of hyperbaric oxygen therapy on acute ankle sprains /". Thesis, McGill University, 2000. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=31140.
Testo completoWilliamson, Raymond Allan. "An experimental study of the use of hyperbaric oxygen treatment to reduce the side effects of radiation treatment for malignant disease". University of Western Australia. School of Anatomy and Human Biology, 2007. http://theses.library.uwa.edu.au/adt-WU2007.0063.
Testo completoCollins, Michael J. "The Use of Hyperbaric Oxygenation Therapy to Change Cerebral Metabolism Rates in Patients with Chronic Brain Damage". NSUWorks, 2009. http://nsuworks.nova.edu/cps_stuetd/20.
Testo completoMoolman, Francis Sean. "Oxygen carriers for a novel bio-artificial liver support system". Pretoria : [s.n.], 2003. http://upetd.up.ac.za/thesis/available/etd-09092004-162043.
Testo completoTitle from opening screen (viewed Oct. 06, 2004). Summaries in English and Afrikaans. Includes bibliographical references (leaves 144-151).
Leite, Magno Santos. "Alterações estruturais de corpos carotídeos de ratos expostos à hiperoxigenação hiperbárica". Universidade de São Paulo, 2006. http://www.teses.usp.br/teses/disponiveis/5/5144/tde-13022008-100815/.
Testo completoWe sough to confirm the existence of structural alterations in rat carotid bodies exposed to hyperoxia that could explain the attenuation of the ventilatory hypoxic drive (HD) by hyperoxic conditions described in the literature. We also tested the hypothesis of there being a deviation of blood flow toward intraglomic capillaries in situations of hyperbaric oxygenation (HBO).15 adult male Wistar rats were divided in 3 groups and exposed to O2 at 2.4 ATA for 6 hours, at 3.0 ATA for 6 hours and to air at 1.0 ATA (control group). The results obtained through histological and morphometric analysis showed: a) no alteration in the architecture of the carotid bodies, but the cytoplasm of the cells exposed to the highest dose were disarranged, a feature confirmed by electron microscopy; b) a significant increase in volume density of capillaries filled out by red blood cells but not of interstitial stroma in the group exposed to O2 at the highest dose; c) a significant vasoconstriction of larger arterioles in all doses of oxygen employed in the study and of smaller arterioles at the highest dose of O2; d) significant variations in the proportion of glomic cell variants in the group exposed to the lowest dose of O2; e) mitochondria with few cristae, so in glomic cells as in nerve-endings, although in the former they were very deformed; f) cytoplasmic membranous proliferation with an increase of endoplasmic reticulum and Golgi apparatus in glomic and sustentacular cells. These results suggest a deviation of blood flow from more calibrated vessel toward intraglomic capillaries, confirming our initial hypothesis and indicate that oxygen, depending on the dose used, exerts an important toxic effect on rat carotid body with significant alterations of glomic cell and nerve-endings ultrastructure.
Marcon, Raphael Martus. "Estudo dos efeitos do monossialogangliosídio (GM1) e da câmara de oxigenoterapia hiperbárica na lesão medular aguda em ratos". Universidade de São Paulo, 2009. http://www.teses.usp.br/teses/disponiveis/5/5140/tde-08032010-103409/.
Testo completoThe objectives were to evaluate the effect of GM1 ganglioside, hyperbaric oxygen, and both in combination, in the treatment of experimental spinal cord lesions in rats. Thirty-two Wistar rats with spinal cord lesions were divided into four groups: one group received GM1 ganglioside, one was submitted to hyperbaric oxygen therapy, the third received both treatments, and the fourth received no treatment (control). There were no significant differences between the groups in the histological analysis, for any of the variables (necrosis, hemorrhage, hyperemia, cystic degeneration, p > 0.06). Neither were there any significant differences in the comparison of left and right sides in the functional tests (p > 0.06 for all). No significant differences were found in the locomotor ratings, in the comparison of groups at 2 days, 7 days, 21 days and 28 days after the surgical procedure. However, in the evaluation on day 14, Group 3, which received the combined therapy, showed a significantly higher BBB score than the other groups (p = 0.015). In the evaluation on day 28, there was a trend to Group 1 (GM1) and 3 (combined therapy) showed a higher BBB score than the group 4 (control), but with no significance (p=0,057). In conclusion, the is a benefit in the use of GM1 ganglioside, but with no significance and the therapeutic effect of GM1 in locomotor evaluation of rats submitted to spinal cord lesion is anticipated by hyperbaric oxygen therapy.
Nakutis, Fernanda Serafim. "Avaliação do estresse oxidativo em modelo experimental da doença de Crohn submetido ao tratamento de oxigênio hiperbárico". Universidade de São Paulo, 2015. http://www.teses.usp.br/teses/disponiveis/5/5168/tde-27102015-123155/.
Testo completoIntroduction: The Knowledge about the physiopathogenesis of inflammatory bowel disease (IBD) has evolved over the last decades. However, although therapies have improved, 2/3 of the cases still need alternative drugs and support therapy. The constant search for alternative treatments and more effective modalities has brought to light some promising strategies, as the use of hyperbaric oxygen (HBO). The use of such therapy surged rapidly in the 90´s showing good results and few side effects being, later on, \"forgotten\" due to the efficacy shown by the use of biological therapies. Objective: This study aimed to evaluate the effects of HBO treatment in mice with chemically induced colitis, using 2,4,6 trinitrobenzene sulfonic acid 2,5% (TNBS) over the evaluation of the animals, histological analysis, inflammatory profile through cytokines IL-4, IL-10, IL-12, IL-13, IL-17, TNF- alfa and interferon y, and also the activity of the antioxidant enzymes superoxide dismutase (SOD), gluthatione peroxidase (GPx) and gluthatione reductase (GR) in intestine of mice. Methodology: Male mice were divided into 6 groups, in group 1, colitis was induced by TNBS 2,5%+ Ethanol 35%, named as TNBS, group 2 also received TNBS 2,5%+ Ethanol 35% + HBO, named as TNBS+HBO, group 3 received only Ethanol 35%, named as ALCOHOL, group 4 received Ethanol 35% associated with HBO, named as ALCOHOL+HBO, group 5 received Saline (NaCl 0,9%), named as SALINE and group 6 received Saline combined with HBO, named as SALINE+HBO. During the treatment the animals were evaluated daily. The treatment with HBO was performed for 4 days and at the end, the samples of the final portion of the bowel were removed and stored for histological, antioxidant enzymes and cytokines analysis. Results: This study has shown that the HBO promoted a significant improve on these animals clinical status. The group which received TNBS showed a 12,71% body weight loss after 24 hours, and by the end of the experimental period the average weight loss was 14,63%. On the other hand, the animals treated with HBO showed only 7,52% weight loss during the first 24 hours, having recovered the weight lost in 5,58% by the end of the experimental period. The histological evaluation of the TNBS+HBO group presented a significant improvement when compared with TNBS group. The treatment with HBO increased the activity of the antioxidant enzymes SOD and GPx in all groups, being only significant among the groups TNBS vs TNBS+HBO, difference in the activity of GR was not observed among the groups. Regarding the inflammatory profile, it was observed that the treatment with HBO promoted the decrease of pro-inflammatory cytokines INFy, IL-12, IL-17 and TNFalfa, as well as the increase of anti-inflammatory cytokines IL-4 and IL-10, while IL-13 was not affected. These data represents, in experimental model, the potential anti-inflammatory effect and the increase of the enzymatic antioxidant defenses promoted by the HBO
Caviness, James A. "Stress biomarkers in a rat model of decompression sickness /". Download the thesis in PDF, 2005. http://www.lrc.usuhs.mil/dissertations/pdf/Caviness2005.pdf/.
Testo completoPirone, Christy Joan. "The hyperbaric incident monitoring study (HIMS) : an international study of incidents occuring in hyperbaric medicine units". Thesis, 2000. http://hdl.handle.net/2440/110129.
Testo completoThesis (M.Clin.Sc.) -- University of Adelaide, Dept. of Clinical Nursing, 2001.
Wilkinson, David Cameron. "Hyperbaric oxygen and insulin sensitivity". Thesis, 2020. http://hdl.handle.net/2440/129616.
Testo completoThesis (Ph.D.) -- University of Adelaide, Adelaide Medical School, 2020
Lai, Chih-Hsun, e 賴志勛. "Effect of Hyperbaric Oxygenation after Sepsis in Diabetic Individuals". Thesis, 2013. http://ndltd.ncl.edu.tw/handle/41289840804674347528.
Testo completo慈濟大學
生理暨解剖醫學碩士班
101
Diabetes is a chronic disease that occurs either when the pancreas does not produce enough insulin or when the body cannot effectively use the insulin it produces. Hyperglycemia is a common effect of uncontrolled diabetes and over time leads to serious damage to many of the body's systems, especially the nerves, blood vessels and immunity system. If occurs of infection in diabetic individuals, it is possible to get sepsis. Sepsis is a potentially serious medical condition that is characterized by a whole-body inflammatory state. It is a serious disease. In Taiwan, every year about three thousand eight hundred people die with sepsis. Hyperbaric oxygen (HBO) therapy is a well-established therapeutic approach increasing oxygen concentration in all tissues; improving blood flow to compromised organs; stimulating angiogenesis; increasing antioxidant enzyme expression; and aiding in the suppression of infections by enhancing white blood cell action. Our previous data showed hyperbaric oxygenation pretreatment could attenuate cardiovascular neural dysfunction in diabetic rats with sepsis, so we tried to study the effect of hyperbaric oxygenation in diabetic individuals with sepsis which is relevant to the clinical situation. In conclusion, given hyperbaric oxygen in diabetic individuals after sepsis could improve the survival rate and attenuate the decline of heart rate variability and blood pressure variability. Keyworld: hyperbaric oxygen, autonomic neuropathy, diabetic mellitus, sepsis, heart rate variability, blood pressure variability
Hodge, Rachel E. "Coping during hyperbaric oxygen therapy : predictors and intervention : a thesis submitted in partial fulfilment of the requirements for the degree of Master of Science in Psychology at the University of Canterbury /". 2008. http://hdl.handle.net/10092/2167.
Testo completoTypescript (photocopy). "Supervised by Associate Professor Neville Blampied, Dr Lois Surgenor, and Dr Mike Davis." Includes bibliographical references (leaves 125-138). Also available via the World Wide Web.
MAO, SHIH-PENG, e 毛士鵬. "The effects and possible mechanisms of hyperbaric oxygenation on central nervous system". Thesis, 1999. http://ndltd.ncl.edu.tw/handle/80329212769222850997.
Testo completo國防醫學院
海底醫學研究所
87
Hyperbaric oxygenation (HBO2) has been used in clinical treatment of a number of medical conditions such as decompression sickness, CO intoxication, burn injury and so forth. The use of HBO2 is limited by its toxicity, in which free radicals formation, nitric oxide and glutamate systems seem to be involved. In this study, using both in vivo and in vitro approaches, we investigated whether free radical formation and neuronal injury are induced following single exposure to HBO2. First, SD rats, CD-1 and C57BL/6 mice were used, and the experimental group was exposed to 6ATA 100% O2, while control group to 3.5% O2 mixed air 6ATA for 20 to 30 minutes. In the HBO2-induced seizure experiment, the SD rats were pretreated with several doses of the presumable NMDA antagonist dextromethorphan prior to HBO2 exposure and recorded the seizure latency of each animal. In the in vitro study, neonatal SD rat primary cortical cultures were divided into several groups to test the possible mechanisms of HBO2-induced CNS toxicity. Our data showed that HBO2 exposure did not alter the content of dopamine in the striatum of three species of animals, with significant decrease in DOPAC of striatum of CD-1 mice. HBO2 increased the formation of 2,3-DHBA, a marker for hydroxyl radical, in the cortex of SD rats, and in the striatum and hippocampus of CD-1 mice, but not in any above-mentioned areas of C57BL/6 mice. The HBO2 exposure also decreased the formation of nitric oxide in the striatum of CD-1 mice. We also found that rats pretreated with dextromethorphan shortened the seizure latency during HBO2 exposure at higher doses. In vitro study demonstrated that HBO2 exposure resulted in lactate dehydrogenase (LDH) release and methylthiazol tetrazolium (MTT) reduction. Pretreatment with MK-801, L-NAME and DMTU protected the cells against the HBO2-induced CNS toxicity, while the SNP exacerbated the toxicity. In summary, in vivo data suggest that HBO2 can induce the formation of hydroxyl radical in specific brain areas. Additionally, there exists a species difference in the vulnerability to HBO2 exposure. In vitro studies suggest that the glutamate, nitric oxide and hydroxyl radical systems are involved in the HBO2-induced CNS toxicity. However, the exact mechanism of the effect of dextromethorphan remains to be elucidated. It is speculated that this agent may first inhibit NMDA receptor that in turn inhibits GABA interneurons and leads to the lowering of seizure threshold, or increase the cerebral blood flow that results in the increased formation of free radicals during HBO2 exposure.
Cavaco, Tânia Cristina Castro Santos. "Effects of hyperbaric oxygenation on histological healing of surgically repaired rotator cuff tears in rabbits". Master's thesis, 2016. https://repositorio-aberto.up.pt/handle/10216/89339.
Testo completoCavaco, Tânia Cristina Castro Santos. "Effects of hyperbaric oxygenation on histological healing of surgically repaired rotator cuff tears in rabbits". Dissertação, 2016. https://repositorio-aberto.up.pt/handle/10216/89339.
Testo completoAl-Hallaq, Hania A. "Measurement of changes in tumor oxygenation by high spectral and spatial resolution MRI /". 2000. http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&res_dat=xri:pqdiss&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&rft_dat=xri:pqdiss:9977996.
Testo completoLanzinger, Marcella Josephine [Verfasser]. "Cerebral blood flow and cerebrovascular response to intermittent hypercapnia during hyperbaric oxygenation treatment / Marcella Josephine Lanzinger". 2004. http://d-nb.info/972198504/34.
Testo completoLi, Yu-hung, e 李昱宏. "Developing an Experimental Model of Osteoradionecrosis in Rats and Elucidating the Therapeutic Role of Hyperbaric Oxygenation". Thesis, 2012. http://ndltd.ncl.edu.tw/handle/60475417770690621352.
Testo completo慈濟大學
生理暨解剖醫學碩士班
100
The purpose of this study is to develop a model of osteoradionecrosis (ORN) in rats and to clarify the therapeutic role of hyperbaric oxygenation. Osteoradionecrosis in male Sprague–Dawley (SD) rats were induced with synchronous mandibular fractures and irradiation on the left mandible at a dose of 20 Gy. Hyperbaric oxygen (HBO) treatment in two doses of 3.0 or 2.5 atmosphere absolute pressure (ATA) for 60 min was given once daily for 20 days. Computerized tomography (CT) was used to evaluate the morphologic change of mandibles. Fresh mandibular length and weight were measured immediately after sacrifice. H&E and immunohistochemical staining of bone morphogenetic protein-2 (BMP-2) and transforming growth factor-β1 (TGF-β1) were used to demonstrate microscopic derangement and the functional outcome. Body weight loss and hair loss in the mandibular area were noticed weeks after irradiation. Atrophy of the ipsilateral incisor was remarkable in individuals underwent synchronous mandibular fractures and irradiation. CT scan also demonstrated nonunion. Significant changes of mandibular length were found. Fibrosis of bone marrow cavity with empty lacunae was observed in animals with ORN. The progression of incisor atrophy was inhibited by HBO treatment. HBO treatment also demonstrated therapeutic role in microscopic deficits. Immunohistochemical staining showed a reduction of BMP-2 and increased TGF-β1 expression in individuals with ORN. HBO therapy was able to attenuate this functional impairment of ORN. Synchronous mandibular fractures and irradiation at a dose of 20 Gy successfully induces ORN in SD rats. HBO therapy of 3.0 ATA, 60 minutes, 20 dives demonstrates therapeutic benefits in macroscopic, microscopic, and functional protein production perspectives.
Chang, Cheng-Fu, e 張成富. "Neuroprotective Effects of Glial Cell line-Derived Neurotrophic Factor、Bone Morphogenetic Proteins and Hyperbaric Oxygenation against Stroke". Thesis, 2001. http://ndltd.ncl.edu.tw/handle/51295079253067494703.
Testo completo國防醫學院
醫學科學研究所
90
This dissertation research focused on focal cerebral ischemia, using the middle cerebral artery occlusion model in rats and mice. Studies were conducted primarily to examine influences of TGF-β family trophic factors on the sequalae of pathophysiological events. One study examined changes in the receptor for GDNF to test the hypothesis of an endogenous GDNF neuroprotective system.(Time Course Study of GFRa-1 Expression in an Animal Model of Stroke. Sarabi A, Chang CF, Wang Y, Hoffer BJ, and Morales M. Exp Neurol. 2001;170:283-289.) Previous studies have shown that intracerebral administration of glial cell line-derived neurotrophic factor (GDNF) reduces ischemia-mediated cerebral infarction. The biological effects of GDNF are mediated by GDNF-family receptor alpha-1 (GFRa-1) and c-Ret. In this study, we examined the levels of expression of GFRa-1 and c-Ret in a rat model of stroke. Adult Sprague-Dawley rats were anesthetized with chloral hydrate. The right middle cerebral artery was ligated at its distal branch for 90 min. Animals were sacrificed at 0, 6, 12, and 24 h after reperfusion and levels of expression of GFRa-1 and c-Ret mRNA were determined by in situ hybridization histochemistry. We found that GFRa-1 mRNA was up-regulated in CA3, dentate gyrus (DG), cortex, and striatum. The peak of up-regulation in DG was 6 h after reperfusion. GFRa-1 mRNA levels in CA3 were gradually up-regulated over the 24-h reperfusion period. In cortex, GFRa-1 mRNA was up-regulated at all time points; however, the peak of up-regulation was observed at 0 and 24 h after reperfusion. In striatum, an initial up-regulation of GFRa-1 was found at 0 h after ischemia. In striatum, up-regulation of c-Ret mRNA was detected as early as 0 h after reperfusion. A gradual increase was found at 6, 12, and 24 h after reperfusion. In conclusion, our results indicate that there are both regional and temporal differences in up-regulation of GFRa-1 and c-Ret after ischemia. Since GDNF is neuroprotective, up-regulation of GFRa-1 and c-Ret could enhance the responsiveness to GDNF and reduce neuronal damage. The selective up-regulation of GFRa-1 and c-Ret in different brain areas suggests that there may be regional differences in GDNF-induced neuroprotection in stroke. These changes in receptors for GDNF supports the hypothesis of an endogenous GDNF neuroprotective mechanism and extends earlier data showing a parallel upregulation of GDNF itself in stroke. A second study investigated another member of the TGF-β family, termed Bone Morphogenic Protein-6 (BMP-6) in this same rat model of focal ischemia. (Bone Morphogenetic Protein-6 Reduces Ischemia-Induced Brain Damage in Rats. Wang Y, Chang CF, Morales M, Chou J, Chen H-L, Chiang Y-H, Lin S-Z, Cadet J L, Deng X, Wang, J-Y, Chen, S-Y , Kaplan PL, and Hoffer,BJ. Stroke. 2001;32:2170-2178.) Bone morphogenetic protein-6 (BMP-6) and its receptors are expressed in adult and fetal brain.Receptors for BMP-6 are upregulated in adult brain after injury, leading to the suggestion that BMP-6 is involved in the physiological response to neuronal injury. The purpose of my study was to determine whether there was a neuroprotective effect of BMP-6 in vivo and in vitro. Lactate dehydrogenase and microtubule-associated protein-2 (MAP-2) activities were used to determine the protective effect of BMP-6 against H2O2 in primary cortical cultures. The neuroprotective effects of BMP-6 were also studied in chloral hydrate—anesthetized rats. BMP-6 or vehicle was injected into right cerebral cortex before transient right middle cerebral artery (MCA) ligation. Animals were killed for triphenyl-tetrazolium chloride staining, caspase-3 immunoreactivity and enzymatic assays, and TUNEL assay. A subgroup of animals were used for locomotor behavioral assays. Application of H2O2 increased lactate dehydrogenase activity and decreased the density of MAP-2 neurons in culture. Both responses were attenuated by BMP-6 pretreatment. Complementary in vivo studies showed that pretreatment with BMP-6 increased motor performance and generated less cerebral infarction induced by MCA ligation/reperfusion in rats. Pretreatment with BMP-6 did not alter cerebral blood flow or physiological parameters. There was decreased ischemia-induced caspase-3 immunoreactivity, caspase-3 enzymatic activity, and density of TUNEL-positive cells in ischemic cortex in BMP6-treated animals. BMP-6 thus reduces ischemia/reperfusion injury, perhaps by attenuating molecular events underlying apoptosis. Because GDNF and BMP-6 utilize entirely different receptors and intracellular second messengers, these experiments also suggest a “cocktail” of trophic factors may provide optimal neuroprotection in stroke. A third study examined another approach to provide neuroprotection for cerebral ischemia, utilizing hyperbaric oxygen. (Hyperbaric oxygen therapy for treatment of postischemic stroke in adult rats. Chang CF, Niu KC, Hoffer BJ, Wang Y, and Borlongan CV. Exp Neurol. 2000;166:298-306.) The hypothesized efficacy of hyperbaric oxygen (HBO) therapy for treatment of stroke was tested in this study. Adult rats were subjected to occlusion of the middle cerebral artery and subsequently exposed to HBO (3 atm, 2 x 90 min at a 24-h intervals. Animals terminated shortly after the second HBO treatment) or hyperbaric pressure (HBP; 3 atm, 2 x 90 min at a 24-h interval; animals terminated shortly after the second treatment) immediately after the ischemia or after a 60-min delay generally displayed recovery from motor deficits at 2.5 and 24 h of reperfusion. There was also a reduction in cerebral infarction at 24 h of reperfusion compared to ischemic animals subjected to normal atmospheric pressure. While both HBO and HBP treatments promoted beneficial effects, HBO produced more consistent protection than HBP. Treatment with HBO immediately or 60 min after reperfusion produced equally significant attenuations of both cerebral infarction and motor deficits. In contrast, protective effects of HBP treatment against ischemia were noted only when administered immediately after ischemia; there was a significantly reduced infarction volume, but only a trend toward decreased behavioral deficits. The present results demonstrate that HBO and, to some extent HBP, reduce ischemic brain damage and behavioral dysfunctions. Thus, we have validated the use of HBO in clinical situations after acute stroke. The last study in this thesis examined the mechanisms of methamphetamine facilitation of ischemic cerebral injury. (Methamphetamine potentiates ischemia/reperfusion insults after transient middle cerebral artery ligation.Wang Y, Hayashi T, Chang CF, Chiang YH, Tsao LI, Su TP, Borlongan C, and Lin SZ. Stroke. 2001;32:775-82.) Previous studies have indicated that both methamphetamine (MA) and ischemia/reperfusion injuries involve reactive oxygen species formation and activation of apoptotic mechanisms. That MA could have a synergistic or additive effect with stroke-induced brain damage is possible. The purpose of the present study was to investigate whether administration of MA in vivo would potentiate ischemic brain injury. Adult CD-1 mice were pretreated with MA or saline. Each animal was later anesthetized with chloral hydrate and placed in a stereotaxic frame. A subset of animals received intracerebral administration of GDNF. The right middle cerebral artery and bilateral carotids were transiently occluded for 45 minutes. Regional cerebral blood flow was measured by laser Doppler. Animals were sacrificed for triphenyltetrazolium chloride staining and p53 mRNA Northern blot assay after 24 hours of reperfusion. Cortical and striatal GDNF levels were assayed by ELISA. We found that pretreatment with MA increased ischemia-induced cerebral infarction. Ischemia or MA alone enhanced p53 (a pro-apoptotic molecule) mRNA expression. Moreover, MA potentiated expression of p53 mRNA in the ischemic mouse brain. MA pretreatment decreased GDNF levels in ischemic striatum. Intracerebral administration of GDNF before ischemia reduced MA-facilitated infarction. Our data indicate that MA exacerbates ischemic insults in brain, perhaps through the inhibition of GDNF-mediated pathways and suggest that MA may antagonize endogenous neuroprotective pathways as part of its mechanism of action. In addition, MA may also upregulate pro-apoptotic mechanisms, contributing to the ultimate extent of infarction. Taken together, these studies strengthen the hypothesis that apoptotic mechanisms are important in determining the extent of infarction after focal cerebral ischemia. They further provide a preclinical basis for exploring new potential therapies based on trophic proteins, particularly in the TGF-β family.
Fen, Li Guie, e 李桂芬. "The effect of hyperbaric oxygenation in spinal fusion - Using the model of posterolateral intertransverse fusion in rabbits". Thesis, 2000. http://ndltd.ncl.edu.tw/handle/60598469713935718840.
Testo completo長庚大學
機械工程研究所
88
Since hyperbaric oxygen therapy (HBO) has been proved to promote osteogenesis in rabbits’ tibial lengthening , the purpose of this study was to determine the facilitating effect of hbo on bone graft healingin lumbar vertebra. Materials and methods: twenty-four male new zealand rabbits ,weighted 3.5 kg , respectively received a 2 cm2 autogeneous bone graft between the fifth and sixth lumbar transverse processes . 24 rabbits were evenly assigned into two groups, hbo group( experimental group )and normal air group ( control group). Each was then subdivided into 4 -week and 8-week groups containing 6 rabbits separately. From the third day after operation ,the rabbits in experimental groups were exposed to 4-week and 8-week hbo ,2.5 atm ,2 hours a day . While those in the control groups were set in normal air.all the rabbits underwent bone mineral density ( BMD) testing every second week and radiograqphy every fourth week. In the end of the Fourth and eighth week, they were sacrificed for l5 and l6 vertebral bodies and transverse processes ,which were subjects of biomechanical torsion testing. Results: in the hbo group, the average value of bmd and bmc increased most in the sixth week, while ceased from increasing in the eighth week. Radiographic study presented the union rate of bone graft:10/12 in 8-week HBO group, 7/12 in 8-week normal air group ,7/12 in 4-week hbo group, and 3/12 in the 4-week normal air group.Consequently, hbo promoted the union rate of lumbar bone graft.In biomechanical testing, the average values of maximum torsion from 8-week HBO group and normal air group were 3079.8 n-mm and 2661.6 n-mm respectively. A statistical difference existed between them(P=0.042). In the aspect of biomechanical rational analysis, 8-week hbo group had the greatest shearing force modulus( g ), 8-week normal air group owned the second one, 4-week HBO group followed, and 4-week normal air had the mininum g value. Conclusion: the conclusion drawn from three tests and biomechanical rationale analysis supports the hypothesis that HBO will promote the union rate of bone graft in rabbits’ lumbar vertebra . The bone graft after hbo therapy possess stronger torsion.
Yu, Shi-Yau, e 虞希堯. "Integrative Vascular Medicine:(1)Far-Infrared Light Therapy Increasing Tissue Perfusion;(2)Hyperbaric Oxygenation Increasing Hepatic Ischemic Tolerance". Thesis, 2006. http://ndltd.ncl.edu.tw/handle/94312049912493895071.
Testo completo國立清華大學
分子與細胞生物研究所
94
More than 50% of the populations have used complementary and alternative medicine (CAM) or integrative medicine (IM) at least once. From a functional standpoint, CAM/IM may be defined as interventions neither taught widely in medical schools nor generally available in hospitals. Well-designed medical researches and clinical trials are urgently needed to test the safety and efficacy of CAM/IM. Far-infrared (FIR) light therapy and hyperbaric oxygen (HBO) therapy are two emerging areas of CAM, and get much advances and utilization recently. Skin microcirculation plays an important role in chronic wound healing and hepatic ischemia-reperfusion (I/R) injury is a lethal complication met in transplantation and resection surgeries. The purpose of this study was to validate the role of FIR and HBO in skin microcirculation and hepatic I/R injury. Sixty rats were used in the FIR study. A WSTM TY301 far-infrared emitter was placed 20cm over the rats. Skin temperature and blood flow were continuously measured. Under laboratory control, the abdominal skin temperature steadily increased to 38 to 39°C, and was kept at constant temperature. The results showed that there was no significant change of skin blood flow during FIR treatment. Skin blood flow increased significantly soon after the removal of FIR emitter. The stimulating effect of skin blood flow was more significant in the rats treated with FIR for 45 min and could be sustained as long as 60 min. These findings suggested a non-thermic biological effect of FIR on skin microcirculation. The promotive effect of FIR on increasing skin blood flow was not influenced by pretreatment of APP (atropine, propranolol, phentolamine), but was suppressed by L-NAME (an eNOS inhibitor) pretreatment. In conclusion, FIR therapy exerts a NO-related biological effect to increase skin microcirculation in rats. This might bring into perspective the clinical application of FIR to treat ischemic disease by augmenting L-arginine/NO pathway. Daily treatment with one dose-HBO (90 minutes, 2.5ATA) was brought about for male Spraque-Dawley rats for one to three days before an I/R injury of liver. Hepatic expression of heat-shock protein 70 (Hsp70), total concentration of glutathione (GSH), activity of catalase, superoxide dismutase (SOD) and serum AST and ALT were estimated before and after HBO, as well as after I/R injury. The results showed that activity of hepatic catalase was decreased by one dose, but not three doses, of HBO as compared with baseline data. However, hepatic Hsp70 expression fluctuated insignificantly. AST and ALT increase less in rats preconditioned with one dose-HBO as compared with those without HBO or with 3 doses-HBO. These results showed preconditioning by one dose-HBO has protective on rat liver against subsequent ischemia-reperfusion injury. The present study is a scientific validation of CAM/IM in vascular medicine. Integrating FIR therapy and HBO therapy into conventional vascular therapies is beneficial to the patients with insufficient microcirculation and hepatic I/R injury.
Chen, Hong-Ming, e 陳宏銘. "Effects of Hyperbaric Oxygenation Therapy on Lipopolysaccharide-Induced Systemic Inflammatory Response Syndrome Model in Cytokines Change and Protection of Hepatic Failure". Thesis, 2006. http://ndltd.ncl.edu.tw/handle/85119273685136910530.
Testo completo國立臺灣大學
臨床醫學研究所
94
The present study is designed to reveal the possible mechanism and the therapeutic potential for hyperbaric oxygen therapy (HBO) in the treatment of sepsis , also protection effect on liver during sepsis. Immediate HBO treatment ( 2 ATA , 75 min ) was applied to assess the survival rate of septic BALB/c mice induced by LPS within 84 hours period. The changes of plasma TNF-α and IL-10 after LPS challenge were measured. The pathological change of liver at 24 hours after LPS challenge treated with/without HBO treatment were also studied. The experimental results demonstrates that: (1) immediate HBO exposure after LPS challenge increases the survival rate of mice from 25% to 75%, (2) the plasma concentration of TNF-α after LPS challenge is reduced by immediate HBO treatment, (3) HBO treatment may protect liver from septic injury. In conclusion , HBO treatment exerts beneficial effects on sepsis induced by LPS in BALB/c mouse. The possible mechanism may be due to immunomodulation effect of HBO on immune system.