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Letteratura scientifica selezionata sul tema "Human cerebral organoides"

Autore: Grafiati

Pubblicato: 16 novembre 2024

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Articoli di riviste sul tema "Human cerebral organoides"

Logan, Sarah, Thiago Arzua, Yasheng Yan, Congshan Jiang, Xiaojie Liu, Lai-Kang Yu, Qing-Song Liu e Xiaowen Bai. "Dynamic Characterization of Structural, Molecular, and Electrophysiological Phenotypes of Human-Induced Pluripotent Stem Cell-Derived Cerebral Organoids, and Comparison with Fetal and Adult Gene Profiles". Cells 9, n. 5 (23 maggio 2020): 1301. http://dx.doi.org/10.3390/cells9051301.

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Background: The development of 3D cerebral organoid technology using human-induced pluripotent stem cells (iPSCs) provides a promising platform to study how brain diseases are appropriately modeled and treated. So far, understanding of the characteristics of organoids is still in its infancy. The current study profiled, for the first time, the electrophysiological properties of organoids at molecular and cellular levels and dissected the potential age equivalency of 2-month-old organoids to human ones by a comparison of gene expression profiles among cerebral organoids, human fetal and adult brains. Results: Cerebral organoids exhibit heterogeneous gene and protein markers of various brain cells, such as neurons, astrocytes, and vascular cells (endothelial cells and smooth muscle cells) at 2 months, and increases in neural, glial, vascular, and channel-related gene expression over a 2-month differentiation course. Two-month organoids exhibited action potentials, multiple channel activities, and functional electrophysiological responses to the anesthetic agent propofol. A bioinformatics analysis of 20,723 gene expression profiles showed the similar distance of gene profiles in cerebral organoids to fetal and adult brain tissues. The subsequent Ingenuity Pathway Analysis (IPA) of select canonical pathways related to neural development, network formation, and electrophysiological signaling, revealed that only calcium signaling, cyclic adenosine monophosphate (cAMP) response element-binding protein (CREB) signaling in neurons, glutamate receptor signaling, and synaptogenesis signaling were predicted to be downregulated in cerebral organoids relative to fetal samples. Nearly all cerebral organoid and fetal pathway phenotypes were predicted to be downregulated compared with adult tissue. Conclusions: This novel study highlights dynamic development, cellular heterogeneity and electrophysiological activity. In particular, for the first time, electrophysiological drug response recapitulates what occurs in vivo, and neural characteristics are predicted to be highly similar to the human brain, further supporting the promising application of the cerebral organoid system for the modeling of the human brain in health and disease. Additionally, the studies from these characterizations of cerebral organoids in multiple levels and the findings from gene comparisons between cerebral organoids and humans (fetuses and adults) help us better understand this cerebral organoid-based cutting-edge platform and its wide uses in modeling human brain in terms of health and disease, development, and testing drug efficacy and toxicity.

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Estridge, R. Chris, Jennifer E. O’Neill e Albert J. Keung. "Matrigel Tunes H9 Stem Cell-Derived Human Cerebral Organoid Development". Organoids 2, n. 4 (5 ottobre 2023): 165–76. http://dx.doi.org/10.3390/organoids2040013.

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Human cerebral organoids are readily generated from human embryonic stem cells and human induced pluripotent stem cells and are useful in studying human neurodevelopment. Recent work with human cerebral organoids have explored the creation of different brain regions and the impacts of soluble and mechanical cues. Matrigel is a gelatinous, heterogenous mixture of extracellular matrix proteins, morphogens, and growth factors secreted by Engelbreth-Holm-Swarm mouse sarcoma cells. It is a core component of almost all cerebral organoid protocols, generally supporting neuroepithelial budding and tissue polarization; yet, its roles and effects beyond its general requirement in organoid protocols are not well understood, and its mode of delivery is variable, including the embedding of organoids within it or its delivery in soluble form. Given its widespread usage, we asked how H9 stem cell-derived hCO development and composition are affected by Matrigel dosage and delivery method. We found Matrigel exposure influences organoid size, morphology, and cell type composition. We also showed that greater amounts of Matrigel promote an increase in the number of choroid plexus (ChP) cells, and this increase is regulated by the BMP4 pathway. These results illuminate the effects of Matrigel on human cerebral organoid development and the importance of delivery mode and amount on organoid phenotype and composition.

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He, Zhisong, Ashley Maynard, Akanksha Jain, Tobias Gerber, Rebecca Petri, Hsiu-Chuan Lin, Malgorzata Santel et al. "Lineage recording in human cerebral organoids". Nature Methods 19, n. 1 (30 dicembre 2021): 90–99. http://dx.doi.org/10.1038/s41592-021-01344-8.

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AbstractInduced pluripotent stem cell (iPSC)-derived organoids provide models to study human organ development. Single-cell transcriptomics enable highly resolved descriptions of cell states within these systems; however, approaches are needed to directly measure lineage relationships. Here we establish iTracer, a lineage recorder that combines reporter barcodes with inducible CRISPR–Cas9 scarring and is compatible with single-cell and spatial transcriptomics. We apply iTracer to explore clonality and lineage dynamics during cerebral organoid development and identify a time window of fate restriction as well as variation in neurogenic dynamics between progenitor neuron families. We also establish long-term four-dimensional light-sheet microscopy for spatial lineage recording in cerebral organoids and confirm regional clonality in the developing neuroepithelium. We incorporate gene perturbation (iTracer-perturb) and assess the effect of mosaic TSC2 mutations on cerebral organoid development. Our data shed light on how lineages and fates are established during cerebral organoid formation. More broadly, our techniques can be adapted in any iPSC-derived culture system to dissect lineage alterations during normal or perturbed development.

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Gomez-Jones, Tashaé, e Robert M. Kao. "Ethical Dimensions of Human Organoids Research". American Biology Teacher 83, n. 9 (novembre 2021): 575–78. http://dx.doi.org/10.1525/abt.2021.83.9.575.

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Over the past decade, the development of three-dimensional mammalian cell organization—called human organoids—from stem cells has provided a framework for future clinical therapies. As human organoid research progresses, we also need to keep in mind the cross-cultural and ethical dimensions of human organoids research. Our review article aims to examine the ethical dimensions of cerebral human organoids and provide an ethical framework guide within human organoids research.

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Bao, Zhongyuan, Kaiheng Fang, Zong Miao, Chong Li, Chaojuan Yang, Qiang Yu, Chen Zhang, Zengli Miao, Yan Liu e Jing Ji. "Human Cerebral Organoid Implantation Alleviated the Neurological Deficits of Traumatic Brain Injury in Mice". Oxidative Medicine and Cellular Longevity 2021 (22 novembre 2021): 1–16. http://dx.doi.org/10.1155/2021/6338722.

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Traumatic brain injury (TBI) causes a high rate of mortality and disability, and its treatment is still limited. Loss of neurons in damaged area is hardly rescued by relative molecular therapies. Based on its disease characteristics, we transplanted human embryonic stem cell- (hESC-) derived cerebral organoids in the brain lesions of controlled cortical impact- (CCI-) modeled severe combined immunodeficient (SCID) mice. Grafted organoids survived and differentiated in CCI-induced lesion pools in mouse cortical tissue. Implanted cerebral organoids differentiated into various types of neuronal cells, extended long projections, and showed spontaneous action, as indicated by electromyographic activity in the grafts. Induced vascularization and reduced glial scar were also found after organoid implantation, suggesting grafting could improve local situation and promote neural repair. More importantly, the CCI mice’s spatial learning and memory improved after organoid grafting. These findings suggest that cerebral organoid implanted in lesion sites differentiates into cortical neurons, forms long projections, and reverses deficits in spatial learning and memory, a potential therapeutic avenue for TBI.

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Camp, J. Gray, Farhath Badsha, Marta Florio, Sabina Kanton, Tobias Gerber, Michaela Wilsch-Bräuninger, Eric Lewitus et al. "Human cerebral organoids recapitulate gene expression programs of fetal neocortex development". Proceedings of the National Academy of Sciences 112, n. 51 (7 dicembre 2015): 15672–77. http://dx.doi.org/10.1073/pnas.1520760112.

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Cerebral organoids—3D cultures of human cerebral tissue derived from pluripotent stem cells—have emerged as models of human cortical development. However, the extent to which in vitro organoid systems recapitulate neural progenitor cell proliferation and neuronal differentiation programs observed in vivo remains unclear. Here we use single-cell RNA sequencing (scRNA-seq) to dissect and compare cell composition and progenitor-to-neuron lineage relationships in human cerebral organoids and fetal neocortex. Covariation network analysis using the fetal neocortex data reveals known and previously unidentified interactions among genes central to neural progenitor proliferation and neuronal differentiation. In the organoid, we detect diverse progenitors and differentiated cell types of neuronal and mesenchymal lineages and identify cells that derived from regions resembling the fetal neocortex. We find that these organoid cortical cells use gene expression programs remarkably similar to those of the fetal tissue to organize into cerebral cortex-like regions. Our comparison of in vivo and in vitro cortical single-cell transcriptomes illuminates the genetic features underlying human cortical development that can be studied in organoid cultures.

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Yakoub, Abraam M., e Mark Sadek. "Development and Characterization of Human Cerebral Organoids". Cell Transplantation 27, n. 3 (marzo 2018): 393–406. http://dx.doi.org/10.1177/0963689717752946.

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Studies of human neurodevelopmental disorders and stem cell–based regenerative transplants have been hampered by the lack of a model of the developing human brain. Stem cell–derived neurons suffer major limitations, including the ability to recapitulate the 3-dimensional architecture of a brain tissue and the representation of multiple layers and cell types that contribute to the overall brain functions in vivo. Recently, cerebral organoid technology was introduced; however, such technology is still in its infancy, and its low reproducibility and limitations significantly reduce the reliability of such a model as it currently exists, especially considering the complexity of cerebral-organoid protocols. Here we have tested and compared multiple protocols and conditions for growth of organoids, and we describe an optimized methodology, and define the necessary and sufficient factors that support the development of optimal organoids. Our optimization criteria included organoids’ overall growth and size, stratification and representation of the various cell types, inter-batch variability, analysis of neuronal maturation, and even the cost of the procedure. Importantly, this protocol encompasses a plethora of technical tips that allow researchers to easily reproduce it and obtain reliable organoids with the least variability, and showcases a robust array of approaches to characterize successful organoids. This optimized protocol provides a reliable system for genetic or pharmacological (drug development) screens and may enhance understanding and therapy of human neurodevelopmental disorders, including harnessing the therapeutic potential of stem cell–derived transplants.

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Berdenis van Berlekom, Amber, Raphael Kübler, Jeske W. Hoogeboom, Daniëlle Vonk, Jacqueline A. Sluijs, R. Jeroen Pasterkamp, Jinte Middeldorp et al. "Exposure to the Amino Acids Histidine, Lysine, and Threonine Reduces mTOR Activity and Affects Neurodevelopment in a Human Cerebral Organoid Model". Nutrients 14, n. 10 (23 maggio 2022): 2175. http://dx.doi.org/10.3390/nu14102175.

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Evidence of the impact of nutrition on human brain development is compelling. Previous in vitro and in vivo results show that three specific amino acids, histidine, lysine, and threonine, synergistically inhibit mTOR activity and behavior. Therefore, the prenatal availability of these amino acids could be important for human neurodevelopment. However, methods to study the underlying mechanisms in a human model of neurodevelopment are limited. Here, we pioneer the use of human cerebral organoids to investigate the impact of amino acid supplementation on neurodevelopment. In this study, cerebral organoids were exposed to 10 mM and 50 mM of the amino acids threonine, histidine, and lysine. The impact was determined by measuring mTOR activity using Western blots, general cerebral organoid size, and gene expression by RNA sequencing. Exposure to threonine, histidine, and lysine led to decreased mTOR activity and markedly reduced organoid size, supporting findings in rodent studies. RNA sequencing identified comprehensive changes in gene expression, with enrichment in genes related to specific biological processes (among which are mTOR signaling and immune function) and to specific cell types, including proliferative precursor cells, microglia, and astrocytes. Altogether, cerebral organoids are responsive to nutritional exposure by increasing specific amino acid concentrations and reflect findings from previous rodent studies. Threonine, histidine, and lysine exposure impacts the early development of human cerebral organoids, illustrated by the inhibition of mTOR activity, reduced size, and altered gene expression.

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Shnaider, T. A. "Cerebral organoids: a promising model in cellular technologies". Vavilov Journal of Genetics and Breeding 22, n. 2 (8 aprile 2018): 168–78. http://dx.doi.org/10.18699/vj18.344.

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The development of the human brain is a complex multi-stage process including the formation of various types of neural cells and their interactions. Many fundamental mechanisms of neurogenesis have been established due to the studying of model animals. However, significant differences in the brain structure compared to other animals do not allow considering all aspects of the human brain formation, which could play the main role in the development of unique cognitive abilities for human. Four years ago, Lancaster’s group elaborated human pluripotent stem cell-derived three-dimensional cerebral organoid technology, which opened a unique opportunity for researchers to model early stages of human neurogenesis in vitro. Cerebral organoids closely remodel many endogenous brain regions with specific cell composition like ventricular zone with radial glia, choroid plexus, and cortical plate with upper and deeper-layer neurons. Moreover, human brain development includes interactions between different brain regions. Generation of hybrid three-dimensional cerebral organoids with different brain region identity allows remodeling some of them, including long-distance neuronal migration or formation of major axonal tracts. In this review, we consider the technology of obtaining human pluripotent stem cell-derived three-dimensional cerebral organoids with different modifications and with different brain region identity. In addition, we discuss successful implementation of this technology in fundamental and applied research like modeling of different neurodevelopmental disorders and drug screening. Finally, we regard existing problems and prospects for development of human pluripotent stem cell-derived threedimensional cerebral organoid technology.

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Peng, Xiyao, Lei Wu, Qiushi Li, Yuqing Ge, Tiegang Xu e Jianlong Zhao. "An Easy-to-Use Arrayed Brain–Heart Chip". Biosensors 14, n. 11 (22 ottobre 2024): 517. http://dx.doi.org/10.3390/bios14110517.

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Multi-organ chips are effective at emulating human tissue and organ functions and at replicating the interactions among tissues and organs. An arrayed brain–heart chip was introduced whose configuration comprises open culture chambers and closed biomimetic vascular channels distributed in a horizontal pattern, separated from each other by an endothelial barrier based on fibrin matrix. A 300 μm-high and 13.2 mm-long endothelial barrier surrounded each organoid culture chamber, thereby satisfying the material transport requirements. Numerical simulations were used to analyze the construction process of fibrin barriers in order to optimize the structural design and experimental manipulation, which exhibited a high degree of correlation with experiment results. In each interconnective unit, a cerebral organoid, a cardiac organoid, and endothelial cells were co-cultured stably for a minimum of one week. The permeability of the endothelial barrier and recirculating perfusion enabled cross talk between cerebral organoids and cardiac organoids, as well as between organoids and endothelial cells. This was corroborated by the presence of cardiac troponin I (cTnI) in the cerebral organoid culture chamber and the observation of cerebral organoid and endothelial cells invading the fibrin matrix after one week of co-culture. The arrayed chip was simple to manipulate, clearly visible under a microscope, and compatible with automated pipetting devices, and therefore had significant potential for application.

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Più fonti

Tesi sul tema "Human cerebral organoides"

Wimmer, Ryszard. "Migration of neural stem cells during human neocortical development". Electronic Thesis or Diss., Université Paris sciences et lettres, 2024. http://www.theses.fr/2024UPSLS016.

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Chez les espèces gyrencéphaliques, et en particulier chez l'homme, la forte augmentation de la taille du néocortex est largement soutenue par une niche neurogénique élargie, la zone sous-ventriculaire externe (oSVZ). Cela est dû en grande partie à l'amplification d'une population de cellules souches neurales, les cellules gliales radiales basales (bRG, également appelées oRG). Les cellules bRG colonisent la zone sous-ventriculaire externe grâce à un mouvement dépendant de l'acto-myosine appelé translocation somale mitotique (MST). Le mécanisme moléculaire exact de la MST, la question de savoir si le cytosquelette des microtubules contrôle également d'autres étapes de la translocation des cellules bRG et la contribution de ces mouvements à la dissémination des cellules bRG dans le néocortex humain en développement sont toutefois inconnus. Ici, en utilisant l'imagerie en direct du tissu fœtal humain de la semaine 14-21 et des organoïdes cérébraux, nous identifions un mode de translocation en deux étapes pour les cellules bRG. En plus de la TMS, les cellules bRG subissent un mouvement dépendant des microtubules pendant l'interphase, que nous appelons translocation somale interphasique (TSI). L'IST est plus lente que la TMS et contrôlée par le complexe LINC qui recrute le moteur moléculaire dynéine et son activateur LIS1 vers l'enveloppe nucléaire pour le transport. Par conséquent, le TSI est affecté dans les organoïdes dérivés de patients LIS1. Nous montrons en outre que la TMS se produit pendant la prométaphase et qu'il s'agit donc d'un événement de translocation du fuseau mitotique. Le TSI et le TMS sont tous deux bidirectionnels, avec un mouvement basal net de 0,57 mm par mois de gestation du fœtus humain.Nous montrons que 85% de ce mouvement dépend de l'IST, qui est à la fois plus polarisé et plus processif que le MST.Enfin, nous démontrons que l'IST et le MST sont conservés dans les cellules de glioblastome liées à bRG et qu'ils interviennent par les mêmes voies moléculaires. Dans l'ensemble, notre travail identifie comment les cellules bRG colonisent le cortex fœtal humain et comment ces mécanismes peuvent être liés à des conditions pathologiques
In gyrencephalic species, and in particular in humans, the strong size increase of the neocortex is largely supported by an expanded neurogenic niche, the outer subventricular zone (oSVZ). This is largely due to the amplification of a neural stem cell population, the basal radial glial cells (bRGs, also known as oRGs). bRG cells colonize the oSVZ through an acto-myosin dependent movement called mitotic somal translocation (MST). The exact molecular mechanism of MST, whether the microtubule cytoskeleton also controls other steps of bRG cell translocation, and the contribution of these movements to bRG cell dissemination into the human developing neocortex are however unknown. Here, using live imaging of gestational week 14-21 human fetal tissue and cerebral organoids, we identify a two-step mode of translocation for bRG cells. On top MST, bRG cells undergo a microtubule-dependent movement during interphase, that we call interphasic somal translocation (IST). IST is slower than MST and controlled by the LINC complex that recruits the dynein molecular motor and its activator LIS1 to the nuclear envelope for transport. Consequently, IST is affected in LIS1 patient derived organoids. We furthermore show that MST occurs during prometaphase and is therefore a mitotic spindle translocation event. MST is controlled by the mitotic cell rounding molecular pathway, that increases the cell cortex stiffness to drive translocation. Both IST and MST are bidirectional with a net basal movement of 0,57 mm per month of human fetal gestation. We show that 85% of this movement is dependent on IST, that is both more polarized and more processive than MST. Finally, we demonstrate that IST and MST are conserved in bRG-related glioblastoma cells and occur through the same molecular pathways. Overall, our work identifies how bRG cells colonize the human fetal cortex, and how these mechanisms can be linked to pathological conditions

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Kitahara, Takahiro. "Axonal Extensions along Corticospinal Tracts from Transplanted Human Cerebral Organoids". Kyoto University, 2021. http://hdl.handle.net/2433/261613.

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SKAROS, ADRIANOS. "CEREBRAL CORTICAL GENERIC CIRCUITS SELECTED IN ANATOMICALLY MODERN HUMAN EVOLUTION: A DISSECTION VIA ORTHOGONAL CRISPR PERTURBATIONS". Doctoral thesis, Università degli Studi di Milano, 2022. https://hdl.handle.net/2434/945932.

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Anatomically modern humans (AMHs) have evolved neural features that differ significantly from those of archaic hominins and underlie their cognitive-behavioural specificities; their evolution, however, has thus far been predominantly inferred from the fossil record and from the comparison of modern and archaic genomes. In doing so, a vast number of genes have been found to harbour changes within protein-coding regions, rendering these different between the AMH genome and Neanderthal and Denisovan genomes. To investigate the functional importance of amino acid changes in the modern human cortex, we prioritise and curate evolutionary- and neurodevelopmentally-relevant genes (15) in order to create a dependency network by combining two orthogonal Cas9 proteins from Streptococcus pyogenes and Staphylococcus aureus, to carry out a dual screen in which one gene is activated whilst a second gene is deleted in the same cell; understanding the direction of information flow is vital for characterising how genetic networks affect phenotypes, but also to fully understand single gene functions. Specifically, we integrate hiPSC-based cortical organoid developmental modelling at single cell resolution, multiplex gene editing and gene network reconstruction to enable the first empirically tested, systems-level definition of the molecular logic underlying our recent cortical evolution. We find that five target genes are central to the functional network of perturbed genes, suggesting that the changes in specific genes between AMHs and archaic hominin indeed play a role in cortical evolution and can help understand neurodevelopment.

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Buchsbaum, Isabel Yasmin [Verfasser], e Silvia [Akademischer Betreuer] Cappello. "Discovering novel mechanisms of human cortical development & disease using in vivo mouse model and in vitro human-derived cerebral organoids / Isabel Yasmin Buchsbaum ; Betreuer: Silvia Cappello". München : Universitätsbibliothek der Ludwig-Maximilians-Universität, 2019. http://d-nb.info/1215499760/34.

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Krefft, Olivia [Verfasser], e Philipp [Akademischer Betreuer] Koch. "Unraveling the pathology of different disease severities in human cerebral organoid models of LIS1-lissencephaly / Olivia Krefft ; Betreuer: Philipp Koch". Heidelberg : Universitätsbibliothek Heidelberg, 2020. http://d-nb.info/1223028062/34.

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Kanton, Sabina. "Dissecting human cortical development evolution and malformation using organoids and single-cell transcriptomics". 2019. https://ul.qucosa.de/id/qucosa%3A71686.

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During the last years, important progress has been made in modeling early brain development using 3-dimensional in vitro systems, so-called cerebral organoids. These can be grown from pluripotent stem cells of different species such as our closest living relatives, the chimpanzees and from patients carrying disease mutations that affect brain development. This offers the possibility to study uniquely human features of brain development as well as to identify gene networks altered in neurological diseases. Profiling the transcriptional landscape of cells provides insights into how gene expression programs have changed during evolution and are affected by disease. Previously, studies of this kind were realized using bulk RNA-sequencing, essentially measuring ensemble signals of genes across potentially heterogeneous populations and thus obscured subtle changes with respect to transient cell states or cellular subtypes. However, remarkable advances during the last years have enabled researchers to profile the transcriptomes of single cells in high throughput. This thesis demonstrates how single-cell transcriptomics can be used to dissect human-specific features of the developing and adult brain as well as cellular subpopulations dysregulated in a malformation of the cortex.

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Capitoli di libri sul tema "Human cerebral organoides"

Daoutsali, Elena, e Ronald A. M. Buijsen. "Establishment of In Vitro Brain Models for AON Delivery". In Methods in Molecular Biology, 257–64. New York, NY: Springer US, 2022. http://dx.doi.org/10.1007/978-1-0716-2010-6_17.

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AbstractProgress in stem cell biology has made it possible to generate human-induced pluripotent stem cells (hiPSC) that can be differentiated into complex, three-dimensional structures, where the cells are spatially organized. To study brain development, Lancaster and colleagues developed an hiPSC-derived three-dimensional organoid culture system, termed cerebral organoids, that develop various discrete, although interdependent, brain regions. Here we describe in detail the generation of cerebral organoids using a modified version of the culture protocol.

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Lavazza, Andrea. "Human Cerebral Organoids: Evolving Entities and Their Moral Status". In Advances in Neuroethics, 65–95. Cham: Springer International Publishing, 2022. http://dx.doi.org/10.1007/978-3-030-97641-5_4.

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Hester, Mark E., e Alexis B. Hood. "Generation of Cerebral Organoids Derived from Human Pluripotent Stem Cells". In Neuromethods, 123–34. New York, NY: Springer New York, 2017. http://dx.doi.org/10.1007/978-1-4939-7024-7_8.

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Gabriel, Markus. "Could a Robot Be Conscious? Some Lessons from Philosophy". In Robotics, AI, and Humanity, 57–68. Cham: Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-030-54173-6_5.

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AbstractIn this chapter, the question whether robots could be conscious is evaluated from a philosophical perspective. The position taken is that the human being is the indispensable locus of ethical discovery. Questions concerning what we ought to do as morally equipped agents subject to normative guidance largely depend on our synchronically and diachronically varying answers to the question of “who we are.” It is argued here, that robots are not conscious and could not be conscious, where consciousness is understood as a systemic feature of the animal-environment relationship. It is suggested, that ethical reflection yields the result that we ought not to produce cerebral organoids implanted in a robotic “body.”

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De Paola, Massimiliano. "TLR4-Mediated Neuroinflammation in Human Induced Pluripotent Stem Cells and Cerebral Organoids". In The Role of Toll-Like Receptor 4 in Infectious and Non Infectious Inflammation, 119–27. Cham: Springer International Publishing, 2020. http://dx.doi.org/10.1007/978-3-030-56319-6_8.

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Suzuki, Ikuro. "Toxicological Assessment of Drugs Based on Electrical Activities of Human iPSC-Derived Cortical Neurons, Sensory Neurons and Cerebral Organoids". In Current Human Cell Research and Applications, 57–91. Singapore: Springer Nature Singapore, 2024. http://dx.doi.org/10.1007/978-981-97-4256-1_4.

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Kanton, Sabina, Barbara Treutlein e J. Gray Camp. "Single-cell genomic analysis of human cerebral organoids". In Methods in Cell Biology, 229–56. Elsevier, 2020. http://dx.doi.org/10.1016/bs.mcb.2020.03.013.

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Yan, Yasheng, Thiago Arzua, Sarah Logan e Xiaowen Bai. "Isolation and Culture of Human-Induced Pluripotent Stem Cell-Derived Cerebral Organoid Cells". In Methods in Molecular Biology. New York, NY: Springer US, 2020. http://dx.doi.org/10.1007/7651_2020_328.

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Lachman, Herbert M. "Use of cerebral organoids to model environmental and gene x environment interactions in the developing fetus and neurodegenerative disorders". In Phenotyping of Human iPSC-derived Neurons, 173–200. Elsevier, 2023. http://dx.doi.org/10.1016/b978-0-12-822277-5.00006-7.

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Atti di convegni sul tema "Human cerebral organoides"

Yildirim, Murat, Chloe Delepine, Danielle Feldman, Vincent Pham, Stephanie Chou, Jacque Pak Kan Ip, Alexi Nott et al. "Label-free three-photon imaging of intact human cerebral organoids for tracking early events in brain development". In Optics and the Brain. Washington, D.C.: Optica Publishing Group, 2023. http://dx.doi.org/10.1364/brain.2023.bth1b.3.

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We demonstrate label-free three-photon imaging of intact organoids (~2 mm depth) derived from Rett syndrome patients. Long-term imaging of live organoids shows that mutant neurons have shorter migration distances, slower migration speeds and tortuous trajectories.

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Munyeshyaka, Maxime, Sanjay Singh, Joy Gumin, Jing Yang, Daniel Ledbetter e Frederick Lang. "Abstract 3907: Invasive properties of GSCs with knownIDH1status into human cerebral organoids". In Proceedings: AACR Annual Meeting 2020; April 27-28, 2020 and June 22-24, 2020; Philadelphia, PA. American Association for Cancer Research, 2020. http://dx.doi.org/10.1158/1538-7445.am2020-3907.

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Zhang, Jinqiu, Jolene Ooi, Sarah R. Langley, Obed Akwasi Aning, Magdalena Renner, Chit Fang Cheok, Enrico Petretto, Juergen A. Knoblich e Mahmoud A. Pouladi. "A48 Expanded HTT cag repeats disrupt the balance between neural progenitor expansion and differentiation in isogenic human cerebral organoids". In EHDN 2018 Plenary Meeting, Vienna, Austria, Programme and Abstracts. BMJ Publishing Group Ltd, 2018. http://dx.doi.org/10.1136/jnnp-2018-ehdn.46.

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