Tesi sul tema "Hebe – new zealand – identification"

The evolution and maintenance of gender dimorphism was investigated in the New Zealand genus Hebe (Scrophulariaceae). Hebe originated within New Zealand and gender dimorphism is postulated to have evolved separately four times within the genus. Gender was found to be controlled at least partially by nuclear genes for one species of Hebe. Most species are self-compatible, and H. subalpina was shown to exhibit inbreeding depression. The frequency of females was found to be negatively correlated with the amount of fruit set on the polleniferous (or male) morpho Dimorphism is correlated with altitude, and it is hypothesized, based on the pollinators present at different altitudes and degree to which they self-fertilize flowers, that separate sexes evolved in response to the increased level of self pollination that occurs at the higher altitudes. Female frequency varied greatly among the populations of H. strictissima, and was found to be negatively correlated with the relative seed fitness of the males. Fruit set on the males varied greatly in response to plant vigour within plants, among plants within populations, and among populations. Fruit set on females was not correlated with plant vigour, and varied less between populations. Female plants were more prevalent in relatively poor sites where male seed fitness was low. Gender estimates for H. subalpina, based on fruit set, sex ratio, seed production, inbreeding depression, and the rate of self-fertilization, showed that males received less than four per cent of their fitness through ovules. H. subalpina is, therefore, near the dioecy end of gynodioecy. Fruit set on males varies much more than that on females, and is dependent on both the status of individual shoots and the genotype of the plant. Resource allocation patterns differ according to sex, and reflect the way in which the sexes receive their fitness.

In the last decade, Radio Frequency Identification (RFID) technology has been widely used in industries for controlling and monitoring purposes but has limited application in supply chain management. Passive tags are used in commercial offices for access control, while a more active and powerful tag is deployed in highways for electronic road toll collections. Other non-contact, close range methods are popular in subways and other public transport systems. The use of RFID in supply chain management is an emerging technological trend that has attracted a lot of attention in the U.S., Europe, and Asia. This is largely driven by the potential benefits that RFID technology is perceived to deliver in the supply chain, particularly, supply chain visibility. Major retailers and leading corporations around the globe are already testing and implementing RFID, and claiming to have achieved competitive advantages and return on their investments. It is, therefore, important to understand the adoption of RFID in New Zealand supply chains, so that appropriate actions can be taken to ensure that New Zealand companies are not lagging behind. This research study explores and explains the adoption of RFID in supply chains using exploratory survey and case study. The research questions are: 1. What specific benefits can be achieved in organisations by using RFID? 2. What are the barriers to realising these benefits? 3. How and why do organisations adopt or not adopt RFID in supply chains? Three factors were found to be important in the adoption of RFID in New Zealand supply chains. They are the compatibility of RFID with existing organisation's systems and values, the availability of supports to facilitate RFID adoption, and the readiness of the internal and external organisation's environment. It was also found that complexity of the technology and the relative advantage of using RFID were to a certain extent influencing users' perception of RFID compatibility. Dependency on trading partners was found to have some effects on RFID adoption. A theoretical framework of RFID adoption in supply chains is proposed. This framework helps to bring out the important factors in the adoption of RFID in supply chains. While most IS research is focused on individual technology adoption or on intra-organisational technology adoption, this research is focused on technology adoption that involves or has impacts on trading partners, that is, at supply chain level. It provides a three-dimensional evaluation framework which includes technological, organisational, and environmental aspects of inter-organisational technology adoption.
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Leaky buildings are those that show elevated moisture contents of the framing timber, which can subsequently lead to the establishment of fungal and bacterial decay. Prior to this study, the causative agents of the decay in these leaky buildings were unknown, though it was suspected to be one or more species of decay fungi. Therefore, the overall goal of this multi-disciplinary PhD thesis research was to determine the causative agents of decay in leaky buildings of New Zealand in an effort to develop solutions for both their remediation and future prevention. Use of molecular biology methodology and classical mycological techniques based on morphology enabled identification of decay fungi from framing timber and air samples of leaky New Zealand buildings and provided insight into relative importance based on isolation frequency. In most cases, fungi colonising Pinus radiata D. Don were isolated to produce pure cultures. Mycelia from these cultures on agar media were collected to extract DNA. To identify the fungi to the species level, polymerase chain reaction (PCR) with fungal specific DNA primer pairs were performed followed by DNA sequencing of the internal transcribed spacer (ITS) region. Identification was by BLAST (Basic Local Alignment Search Tool) search on sequences in known GenBanks. In total, 421 samples from leaky buildings were processed, predominately untreated P. radiata decayed framing timber and also fibre cement boards and building paper. From these, sixty-eight fungal identifications were made. The only taxa that were isolated with significant frequency were identified as 4 basidiomycete species, as follows, along with the number of times they were isolated from the 421 samples: • Gloeophyllum sepiarium (Wulf.: Fr.) Karst. 13x • Oligoporus placenta (Fries 1865) Gilb. In Ryv.1985 11x • Antrodia sinuosa (Fr.) Karst. 8x • Gloeophyllum trabeum (Fr.) Murr. 4x Although these species were identified repeatedly, in total they represent less than 10% of the total samples and, therefore, it is concluded that the leaky building decay samples represent high fungal biodiversity. An aerial spore study of internal air, wall cavity air and exterior air of leaky buildings was carried out using a Merck MAS-100 instrument which collects spores directly onto selective media plates. Viable fungal aerial spores were detected at every sampling location tested at the leaky buildings, by the criteria of culturing, with a highest mean of 3714 colony-forming units (CFU) per cubic metre found in the cavities of water-damaged walls. This aerial spore study in conjunction with isolation from decayed wood samples from the same leaky buildings enabled identification of G. sepiarium and A. sinuosa at the same test site. The use of carboxymethylcellulose medium further demonstrated the presence of potential cellulose-degrading fungi within and around the location. Overall, the combination of direct sampling of timber and air sampling proved useful for detection of fungal species variability at a multi-unit building. Four decay fungi isolated from New Zealand leaky buildings and two standard control decay fungi (Coniophora puteana and Serpula lacrymans) were submitted to laboratory wood block testing to determine the effectiveness of currently used wood framing preservatives under laboratory conditions before and after a standard leaching regime. P. radiata blocks were treated with water based boron copper azole and solvent based IPBC propiconazole plus tebuconazole (1:1) preservatives and exposed to the basidiomycetes for 12 weeks. Mass loss for the fungal decay-infected samples was recorded of up to 55% for preservative-treated samples, up to 62% mass loss for leached samples and up to 58% mass loss for un-preservative treated samples. Additionally, well defined dosage responses and approximate toxic thresholds were obtained for all preservatives tested. Results suggested that the minimum IPBC retention specified by Hazard Class 1.2 of NZS3640:2003 (0.025% m/m) is on the low side, and demonstrated after the 2 week leaching regime complete loss of efficacy of boron at 0.4% m/m boric acid equivalent (BAE). This PhD research gave a first overview of fungi occurring in New Zealand leaky buildings, and it demonstrated the following key aspects of wood preservation: 1. The isolated test fungus Antrodia sinuosa was more difficult to control with propiconazole plus tebuconazole at retention 0.007% m/m than the known tolerant fungus Oligoporus placenta; 2. Boron at Hazard Class 1.2 retention of 0.4% m/m BAE was not toxic to Oligoporus placenta; 3. Serpula lacrymans exhibited tolerance to the highest retention of 0.06 %m/m tebuconazole plus propiconazole; and 4. Gloeophyllum species appeared susceptible to all wood preservatives. In order to correlate fungal colonisation and wood decay, colonised wood blocks were studied using light microscopy (LM) and field- emission scanning electron microscopy (FE-SEM). Microscopic observations of P. radiata wood blocks following a standard wood decay test of twelve weeks of fungal colonisation by Serpula lacrymans, Antrodia sinuosa, Oligoporus placenta and Gloeophyllum sepiarium revealed that the two microscopic techniques employed were complementary by allowing features such as pit membranes, chlamydospores or S3/S2 compound middle lamella interface to be photographed in greater detail, allowing for more precise analyses and interpretation of key findings, as follows: 1. Brown rot fungi directly target their apical growth towards degraded pit apetures; 2. Reliance on light microscopy and observed birefringence as a tool to record changes in cell wall crystallinity associated with brown rot decay alone could be misleading; 3. Presence of fine (≤ 1 m) to wide (≥ 3.5 m) bore-hole and hyphal size ranges, and nearly unchanged cell wall thickness of all wood/test fungal combinations, confirmed active decay at moderate to late stages; 4. Some ray parenchyma cells for Antrodia sinuosa, Oligoporus placenta and Gloeophyllum sepiarium colonised blocks were intact throughout late stages of decay, outlining that they were not preferentially degraded early in the brown rot decay process, and 5. Presence of bore-holes, clamp and medallion clamp formation and resting spores (chlamydospores and arthrospores) are fungal specific, can aid in their differentiation and identification, and should be recorded during wood decay studies, as especially resting spores are an important factor when planning remediation strategies. In summary, this PhD thesis research provided the first comprehensive investigation into the biodiversity of fungi from leaky New Zealand buildings, identified the dominant species and presented details about their micromorphology and their decay patterns. It also demonstrated substantial differences in efficacy of preservative formulations currently (December 2008) approved for framing treatments in New Zealand and possible deficiencies where framing may be subjected to severe leaching. This study also provided the first comparative analyses of viable fungal aerial spores between leaky wall cavities and the surrounding air environment. Subsequently, this research added to the knowledge of the decay fungal species diversity in and around New Zealand leaky buildings, outlined their capabilities to degrade treated and un-treated P. radiata framing timber and illustrated the efficacy of New Zealand approved wood preservatives for their potential as remedial treatment and future prevention.

Little is known about factors that influence dialect perception and the cues listeners rely on in telling apart two accents. This thesis will shed light on how accurate New Zealanders and Australians are at identifying each other's accents and what vowels they tune in to when doing the task. The differences between New Zealand and Australian English mainly hail from the differing production of the short front vowels, some of which have reached the status of being stereotyped in the two countries. With the help of speech synthesis, an experiment was designed to test the perception of vowels produced in a typically New Zealand and a typically Australian fashion. Forty New Zealanders and sixty Australians took part in the study. Participants were asked to rate words on a scale from 1 (definitely NZ) to 6 (definitely Australian). The words contained one of eight different vowels. Frequency and stereotypicality effects as well as nasality were also investigated. The results demonstrate that dialect identification is a complex process that requires taking into account many different interacting factors of speech perception, social and regional variation of vowels and issues of clear speech versus conversational speech. Although overall performing quite accurately on the task, New Zealanders and Australians seem to perceive each other's speech inherently differently. I argue that this is due to different default configurations of their vowel spaces. Furthermore, a perceptual asymmetry between New Zealanders and Australians concerning the type of vowel has been observed. Reinforcing exemplar models of speech perception, it has also been shown that frequency of a word influences a listener's accuracy in identifying an accent. Moreover, nasality seems to function as an intensifier of stereotypes.

During every summer Olympic Games, the sport of gymnastics rises briefly to the world’s attention as the public admire the incredible skills and feats performed by fit muscular bodies on a range of apparatus. The gymnastics they watch consists of performances in which bodies assemble with apparatus. This thesis utilises an Actor Network Theory (ANT) perspective to follow this assembling of gymnastics in the five codes of competitive gymnastics competed in New Zealand: women’s artistic gymnastics, men’s artistic gymnastics, rhythmic gymnastics, trampolining and competitive aerobics. This thesis is a descriptive ethnography of the world of high performance gymnastics. It begins by examining some of the controversies that have operated to both criticise and rework the sport. Next, the gymnasts are followed through the selection processes that lead them to become members of national squads and teams. It then moves to the training gymnasium and examines the variety of non-human actants that work in the gymnasium to assemble gymnastics. The next two chapters examine how gymnasts are found to enrol and assemble with video technologies and sports science professionals in their efforts to improve performance. Following this, gymnasts are observed to produce a routine at a competition which is translated into a score and ranking through the highly complicated and laborious process of judging. Finally, the thesis concludes with the story of Angela McMillan, New Zealand’s most successful athlete within the gymnastic codes. Throughout are a range of accounts from participants, together with observations, describing attempts to secure the stabilisation of gymnastics as an actor-network that produces internationally successful athletes. All the networks followed involve a continual process of enrolling, un-enrolling, translating and mediating, with power constantly shifting and being shared between various heterogeneous actants including coaches, parents, the national federation and the international federation. At times these networks stabilise with particular actants, such as sports scientists or technologies, being enrolled, while at other times the paths of the networks come to an end as particular assemblages or actants, such as physical ability tests, are no longer enrolled. In contrast to a perception that successful high performance sports include key actors and resources, this thesis shows how the networks that produce high performance gymnasts are highly unpredictable and messy, with humans and non-humans both equally influential in affecting every branch of the networks. Processes such as talent identification, training and judging are found to be complicated and unstable.

The Haast Pass highway has had a long history of instability since it was constructed in 1960. Steep slopes and deeply incised river create an actively changing geomorphic environment making maintaining the highway corridor hazardous and difficult. This thesis study provides the first comprehensive investigation of the highway corridor between the Summit and Thunder Creek Falls using LiDAR and detailed air-photo analysis to provide the basis for geomorphic mapping. Management of slope hazards to date has been based on a reactive approach treating the immediate unstable areas around landslides after they occur. This study presents the first large-scale geomorphological assessment of the highway corridor identifying surface unit type, slope processes and slope hazards in order to facilitate the development of a long-term highway management strategy. Because dense vegetation covers nearly all the slopes above the highway in the study area as as such, it has not been possible to adequately investigate slope geomorphology until the availability of LiDAR. This study is the first to use Light direction and ranging[LiDAR] for corridor hazard mapping beneath dense vegetation in New Zealand. The LiDAR survey was flown by New Zealand Aerial Mapping in January 2014 for the New Zealand Transport Agency and was provided for use in this study. The LiDAR surface model created serves as the basis for mapping surface units and landslide features, enabling the identification of slope processes and landslide hazards. Aerial photos were also used to identify surface unit type and slope processes where vegetation was absent and enabled the activity of slopes to be evaluated. Interpretations made using LiDAR were validated using aerial photography and targeted ground truthing with all ground truthing sites confirming the interpretations made. Large scale geomorphology mapping was undertaken on slopes above the highway on the western side of the valley and showed that there were distinct differences between the southern and northern parts of the highway corridor. Between The Haast Pass Summit and Pipson Creek the slopes are dominated by schist bedrock with regolith confined to small deposits next to the highway and larger deposits in tributary valleys. The slope hazards affecting the highway in this zone are confined to debris sliding and rockfall from regolith deposits and bedrock cliffs next to the highway between Robinson and Pipson Creeks. The slopes between Pipson Creek and the Gates of Haast, in contrast, consist of deep regolith deposits and regolith veneered slopes. Evidence of active and recently active slope processes on the slopes facing the highway confirm the instability is associated with slope hazards including debris flows, debris slides, rock fall and highway collapse. Small-scale detailed evaluations were undertaken at Diana Falls, Ford Creek, The Hinge and the Gates of Haast with the sites selected based on their history of instability and/or their particu- larly hazardous appearance during the large-scale geomorphology and hazard identification. Using the LiDAR surface model surface units and landslide features were identified and mapped with small-scale engineering geomorphology maps. This information was then used to interpret the subsurface geometry and the failure mode/slope processes acting on the slope enabling an assessment of the current stability and future slope development to be made. Diana Falls was found to have scarps and tension cracks running across the regolith covered slope indicating that future landslides from this site will be an ongoing problem. At Ford Creek the landslide was identified as a rock compound slide, but assessments of its current stability and future development were unable to be made. Detailed investigations at The Hinge revealed evidence of a large creeping debris slide and the existence smaller debris slides below the highway through the entire investigation area; the debris slides identified show signs of activity and continued debris sliding will continue to affect the highway in the future. The investigation of the Gates of Haast revealed that the slope instability is not as extensive as it has been in the past, however, recent rock slides and debris flows have continued affect the highway and will continue to pose a hazard in the future. This thesis provides fundamental information required to develop a comprehensive management plan for the Haast Pass highway corridor between the Haast Pass summit and the Gates of Haast. A new landslide management plan has been developed outlining immediate, short-term and long- term options and programmes that should be implemented. Immediate options are steps that can be taken to quickly increase the safety of road users and include moving of highway closure gates and installation of warning signage. Short-term options aim to mitigate landslide hazards where feasible including the installation of rockfall barriers and debris flow attenuators, and lay the groundwork for future avoidance of hazards by undertaking investigations of highway realignment and developing highway closure rainfall thresholds. Long-term options are recommended where landslides will continue to impact the same section of the highway repeatedly and focus on hazard avoidance by building landslide shelters or major highway realignments. The adoption of a management plan ensures security of the highway, protects against loss of life and provides the most cost effective long-term solution to manage the landsliding hazards.

Species are one of the fundamental components of biology and the accurate delimitation of species is important in evolutionary, systematic and ecological studies, yet there is still confusion over how species can be recognised. Examining different characters allows multiple lines of evidence for successful and accurate species delimitation and identification. In this thesis, morphological, genetic and behavioural variation is investigated within an endemic species of ground weta, Hemiandrus pallitarsis, in the North Island, New Zealand. Twelve morphological characters were measured, and mitochondrial cytochrome oxidase I DNA sequences were analysed from populations across the distributional range of H. pallitarsis. Both methods provide no evidence of a species complex within H. pallitarsis. Instead, the morphometric results suggest females are significantly larger than males, and ground weta in Palmerston North are significantly smaller than weta further north. Additionally, genetic analyses found substantial population structuring, large genetic distances, and an historical south to north pattern of movement in the North Island. The pattern of vibratory drumming behaviour followed that predicted by morphology and geographic proximity – drumming signals were more similar between geographically close populations and did not match the patterns of genetic isolation. Overall, this thesis was able to show that H. pallitarsis is morphologically, genetically and behaviourally variable across the North Island.

Thesis (MCIS - Computer and Information Sciences)--AUT University, 2007.
Includes bibliographical references. Also held in print (vii, 94 leaves ; 30 cm.) in the Archive at the City Campus (T 658.514 ZHA).

Each year, the world’s top orange producers output millions of oranges for human consumption. This production is projected to grow by as much as 64 million in 2010 and so the demand for fast, low-cost and precise automated orange fruit grading systems is only deemed to become more increasingly important. There is however an underlying limit to most orange blemish detection algorithms. Most existing statistical-based, structural-based, model-based and transform-based orange blemish detection algorithms are plagued by the following problem: any pixels in an image of an orange having about the same magnitudes for the red, green and blue channels will almost always be classified as belonging to the same category (either a blemish or not). This however presents a big problem as the RGB components of the pixels corresponding to blemishes are very similar to pixels near the boundary of an orange. In light of this problem, this research utilizes a priori knowledge of the local intensity variations observed on rounded convex objects to classify the ambiguous pixels correctly. The algorithm has the effect of peeling-off layers of the orange skin according to gradations of the intensity. Therefore, any abrupt discontinuities detected along successive layers would significantly help identifying skin blemishes more accurately. A commercial-grade fruit inspection and distribution system was used to collect 170 navel orange images. Of these images, 100 were manually classified as good oranges by human inspection and the rest are blemished ones. We demonstrate the efficacy of the algorithm using these images as the benchmarking test set. Our results show that the system garnered 96% correctly classified good oranges and 97% correctly classified blemished oranges. The proposed system is easily customizable as it does not require any training. The fruit quality bands can be adjusted to meet the requirements set by the market standards by specifying an agreeable percentage of blemishes for each band.

Dothistromin (DOTH) is a secondary metabolite produced by the fungal peanut pathogen Passalora arachidicola and pine needle pathogen Dothistroma septosporum. The chemical structure of DOTH is similar to a precursor of aflatoxin (AF) and sterigmatocystin (ST), which are secondary metabolites produced by Aspergillus species. A size fractionated genomic library was made and 11 putative DOTH genes were identified in P. arachidicola. The DOTH genes in P. arachidicola were compared to DOTH genes in D. septosporum as well as to AF and ST genes in Aspergillus species. The DOTH gene products in P. arachidicola showed 73 - 96% amino acid identity to DOTH genes in D. septosporum and 50 - 69% amino acid identity to AF/ST genes in Aspergillus. The DOTH biosynthesis genes in P. arachidicola had similar gene organization and direction of transcription to DOTH biosynthesis genes in D. septosporum and is similar in that 11 putative DOTH genes are separated into three mini-clusters. This differs from the AF/ST clusters in which 25 AF/ST genes are tightly clustered in a 70 kb region. Identification of transcription factor binding sites upstream of DOTH genes in P. arachidicola and D. septosporum suggested similar co-regulation of DOTH gene expression in P. arachidicola and D. septosporum. Tandem and inverted repeat sequences were identified in intergenic regions in the P. arachidicola DOTH gene cluster, but the distribution of those repeats appears to be random. This suggests that the fragmentation of the DOTH biosynthesis gene cluster is not due to retrotransposon activity or recombination between repeat sequences. The DOTH biosynthesis gene clusters in P. arachidicola and D. septosporum could be ancestral to AF/ST biosynthesis clusters in Aspergillus species.

The fungal endophyte Epichloë festucae forms a largely mutualistic association with the ryegrass species Lolium perenne. E. festucae produces a range of bio-protective alkaloids that protect the host grass from herbivory by both mammals and insects. One such alkaloid, Lolitrem B, is a potent mycotoxin and the causative agent of ryegrass staggers in livestock. Ten genes required for biosynthesis of lolitrem B are encoded in the ltm gene cluster. The ltm genes are expressed in a plant-specific manner, with high levels of expression in planta and very low levels of expression in culture. The mechanism regulating ltm gene expression is unknown but it is predicted to involve signalling from the host plant. The ltmM gene was chosen for use in the investigation of ltm gene regulation because the flanking regions do not contain retrotransposon sequence, which surrounds much of the ltm gene cluster. To identify fungal genes involved in the plant-induced expression of ltmM, a mutagenesis and screening system was developed using a PltmM-gusA ‘knock-in’ construct to detect expression from the ltmM promoter. Agrobacterium tumefaciens-mediated T-DNA mutagenesis was used to create a set of mutants with random insertions in the genome. Mutants were then screened for altered PltmM-gusA expression, both in culture and in planta. Three mutants were identified with increased PltmM-gusA expression in culture, however, no mutants were identified with loss of PltmM-gusA expression in planta. This indicates that a mechanism of repression is involved in the plant-induced expression of ltmM, either directly or indirectly. TM mutants of interest were also observed for altered symbiosis phenotypes. Mutants were identified with reduced colonisation rates and altered hyphal growth in planta. Integration sites were identified for two colonisation mutants and the disrupted genes are predicted to be the CTP:cholinephosphate cytidylyltransferase (CCT) gene PCT1 and the mitogen-activated protein kinsase kinase (MAPKK) gene mkk2.

The potential for a gastroenteritis outbreak in a post-earthquake environment may increase because of compromised infrastructure services, contaminated liquefaction (lateral spreading and surface ejecta), and the presence of gastroenteritis agents in the drinking water network. A population in a post-earthquake environment might be seriously affected by gastroenteritis because it has a short incubation period (about 10 hours). The potential for a gastroenteritis outbreak in a post-earthquake environment may increase because of compromised infrastructure services, contaminated liquefaction (lateral spreading and surface ejecta), and the presence of gastroenteritis agents in the drinking water network. A population in a post-earthquake environment might be seriously affected by gastroenteritis because it has a short incubation period (about 10 hours). The aim of this multidisciplinary research was to retrospectively analyse the gastroenteritis prevalence following the February 22, 2011 earthquake in Christchurch. The first focus was to assess whether earthquake-induced infrastructure damage, liquefaction, and gastroenteritis agents spatially explained the recorded gastroenteritis cases over the period of 35 days following the February 22, 2011 earthquake in Christchurch. The gastroenteritis agents considered in this study were Escherichia coli found in the drinking water supply (MPN/100mL) and Non-Compliant Free Associated Chlorine (FAC-NC) (less than <0.02mg/L). The second focus was the protocols that averted a gastroenteritis outbreak at three Emergency Centres (ECs): Burnside High School Emergency Centre (BEC); Cowles Stadium Emergency Centre (CEC); and Linwood High School Emergency Centre (LEC). Using a mixed-method approach, gastroenteritis point prevalence and the considered factors were quantitatively analysed. The qualitative analysis involved interviewing 30 EC staff members. The data was evaluated by adopting the Grounded Theory (GT) approach. Spatial analysis of considered factors showed that highly damaged CAUs were statistically clustered as demonstrated by Moran’s I statistic and hot spot analysis. Further modelling showed that gastroenteritis point prevalence clustering could not be fully explained by infrastructure damage alone, and other factors influenced the recorded gastroenteritis point prevalence. However, the results of this research suggest that there was a tenuous, indirect relationship between recorded gastroenteritis point prevalence and the considered factors: earthquake-induced infrastructure damage, liquefaction and FAC-NC. Two ECs were opened as part of the post-earthquake response in areas with severe infrastructure damage and liquefaction (BEC and CEC). The third EC (CEC) provided important lessons that were learnt from the previous September 4, 2010 earthquake, and implemented after the February 22, 2011 earthquake. Two types of interwoven themes identified: direct and indirect. The direct themes were preventive protocols and indirect themes included type of EC building (school or a sports stadium), and EC staff. The main limitations of the research were Modifiable Areal Units (MAUP), data detection, and memory loss. This research provides a practical method that can be adapted to assess gastroenteritis risk in a post-earthquake environment. Thus, this mixed method approach can be used in other disaster contexts to study gastroenteritis prevalence, and can serve as an appendage to the existing framework for assessing infectious diseases. Furthermore, the lessons learnt from qualitative analysis can inform the current infectious disease management plans, designed for a post-disaster response in New Zealand and internationally Using a mixed-method approach, gastroenteritis point prevalence and the considered factors were quantitatively analysed. A damage profile was created by amalgamating different types of damage for the considered factors for each Census Area Unit (CAU) in Christchurch. The damage profile enabled the application of a variety of statistical methods which included Moran’s I , Hot Spot (HS) analysis, Spearman’s Rho, and Besag–York–Mollié Model using a range of software. The qualitative analysis involved interviewing 30 EC staff members. The data was evaluated by adopting the Grounded Theory (GT) approach. Spatial analysis of considered factors showed that highly damaged CAUs were statistically clustered as demonstrated by Moran’s I statistic and hot spot analysis. Further modelling showed that gastroenteritis point prevalence clustering could not be fully explained by infrastructure damage alone, and other factors influenced the recorded gastroenteritis point prevalence. However, the results of this research suggest that there was a tenuous, indirect relationship between recorded gastroenteritis point prevalence and the considered factors: earthquake-induced infrastructure damage, liquefaction and FAC-NC. Two ECs were opened as part of the post-earthquake response in areas with severe infrastructure damage and liquefaction (BEC and CEC). The third EC (CEC) provided important lessons that were learnt from the previous September 4, 2010 earthquake, and implemented after the February 22, 2011 earthquake. The ECs were selected to represent the Christchurch area, and were situated where potential for gastroenteritis was high. BEC represented the western side of Christchurch; whilst, CEC and LEC represented the eastern side, where the potential for gastroenteritis was high according to the outputs of the quantitative spatial modelling. Qualitative analysis from the interviews at the ECs revealed that evacuees were arriving at the ECs with gastroenteritis-like symptoms. Participants believed that those symptoms did not originate at the ECs. Two types of interwoven themes identified: direct and indirect. The direct themes were preventive protocols that included prolific use of hand sanitisers; surveillance; and the services offered. Indirect themes included the EC layout, type of EC building (school or a sports stadium), and EC staff. Indirect themes governed the quality and sustainability of the direct themes implemented, which in turn averted gastroenteritis outbreaks at the ECs. The main limitations of the research were Modifiable Areal Units (MAUP), data detection, and memory loss. It was concluded that gastroenteritis point prevalence following the February 22, 2011 earthquake could not be solely explained by earthquake-induced infrastructure damage, liquefaction, and gastroenteritis causative agents alone. However, this research provides a practical method that can be adapted to assess gastroenteritis risk in a post-earthquake environment. Creating a damage profile for each CAU and using spatial data analysis can isolate vulnerable areas, and qualitative data analysis provides localised information. Thus, this mixed method approach can be used in other disaster contexts to study gastroenteritis prevalence, and can serve as an appendage to the existing framework for assessing infectious diseases. Furthermore, the lessons learnt from qualitative analysis can inform the current infectious disease management plans, designed for a post-disaster response in New Zealand and internationally.

碩士
長庚大學
醫學生物技術研究所
95
New Zealand Black (NZB) mice spontaneously develop autoimmune hemolytic anemia (AIHA). The major target of the pathogenic red blood cell (RBC) autoantibodies is the anion channel protein Band 3, and CD4 T cells from NZB mice also respond to Band 3. Previous studies from our laboratories showed that Band 3 peptide 861-874 contains a dominant autoreactive helper T-cell epitope with the ability in vivo to modulate the course of AIHA in NZB mice. We have recently found that after inhaling this peptide, the proportion of CD4+CD25+ cells, which are recognized as regulatory T cells were promoted. Adoptive transfer of BLAB/c CD4+CD25+ cells can significantly reduce their RBC bound autoantibody levels. Therefore, in this study, I aim to characterize the CD4+CD25+ regulatory T cells in NZB AIHA development and generate the gene-engineered Foxp3-expressing regulatory cells in vitro. First, we have demonstrated that much lower frequency of regulatory T cells was found in NZB mice as compared to the age-matched BALB/c animals. Second, the expression of Foxp3, the potential marker of T regulatory cells, was reduced in these NZB CD4+CD25+ cells. Most importantly, these CD4+CD25+ cells in NZB mice seemed to lose the suppressive effects on cell proliferation and cytokine production, whereas the CD4+CD25+ cells isolated from BALB/c mice were able to abolish the cell proliferation in response to CD3 stimulation. Finally, the Foxp3-high expressing vector has been constructed and transfected into primary mouse T cells by nucleofection. After transfection with Foxp3, the Foxp3 gene-engineered cells were anergic to anti-CD3/CD28 in vitro. Further studies will test if these gene-engineered regulatory T cells are able to suppress autoimmune diseases.

Organisations face massive changes as they adapt to mergers, takeovers, downsizing or simply adding .com to their name, and all of these changes challenge the organisation's identity. Despite this understanding, little research has been done in the area of how employees react to a change in their organisation's identity, and how such a change may impact upon their own identification. The New Zealand environment represented a unique opportunity to address this gap in the literature. Local expressions of worldwide economic reform have been swift and extensive, with the commercialisation of the tertiary sector presenting a rare opportunity for exploring how change impacts upon the organisation's identity and employees' identification. From this base, the following research question was developed: How do polytechnic tutors interpret the changes to their organisation's identity and their own identification, following education reform in the New Zealand polytechnic sector? A constructivist approach was used" utilising three means of inquiry. Firstly, three separate institutes were selected, with the business school being the site of inquiry for each Case. At each site, repertory grid interviews with long-term employees were conducted to generate data at the level of the individual, and at the level of the organisation. And finally, semi-structured interviews were undertaken with senior management to provide background information on strategic direction and vision. A content analysis of both the repertory grid interviews and the semi-structured interviews provided a rigorous process of analysis. Results from this research make several contributions to our knowledge of identity and identification. The data revealed that the organisation's identity consists of a plurality of identities, with one overarching Meta Identity. Whilst other aspects of identity may change to adapt to the demands of the external environment, the Meta Identity is far more resilient, or 'sticky.' In addition to revealing the hierarchical nature of organisational identity, the data also demonstrate evidence of two paths for employees 'becoming' identified, specifically emulation and affiliation. The findings also indicate that whilst change may not create a reduction in the strength of identification, it may create a change in the need it is serving for the employee. Such changes can be detrimental to both the employee and the organisation.

This thesis presents the analysis of a series of experimental measurements from a selection of springs, geysers and wells at Te Aroha, Rotorua and Orakeikorako in New Zealand aimed at the identification of the frequencies of any characteristic variations with a period of 25 hours or less. The measurements taken from these sources collectively recorded water level, conductivity, temperature, rainfall and barometric pressure. The measurements involve the collection of data with high accuracy instrumentation and high sampling rates of 5 minutes to 1 hour over periods exceeding 1400 hours in both exploited and unexploited hydrothermal systems. The measurements are filtered using both an adaptive wavelet based filter and Fourier analysis to extract the influence of gravity tides, rainfall, and barometric pressure with the residual dominant frequencies being attributed to sub-surface thermodynamic processes. A review of the development of techniques for the determination of the pressure response of geothermal reservoirs to barometric pressure, gravity tides, and rainfall is presented. The fundamentals for the mathematical development of Fourier and wavelet analysis are discussed along with sources of error for both Fourier and wavelet analysis. A sensitivity study of wavelet and Fourier analysis is presented based on the analysis of a series of artificial signals. A system dynamics model of a spring at Orakeikorako is formulated to demonstrate fundamental heat and mass transfer processes and the effect of periodic forcing functions on spring response. This study is of importance in that it provides an understanding of periodic forcing functions derived from high accuracy measurements on geothermal features at high sampling rates over periods exceeding 1400 hours. Once identified, the amplitude and frequency of the forcing functions can be monitored to assist in identifying and quantifying any changes in reservoir characteristics.

Lack of a brief empirically-verified screener for problematic and potentially problematic cannabis use has hampered implementation of a proactive opportunistic cannabis screening and early intervention (SEI) initiative in New Zealand. Addressing this instrumentation need was the primary aim of this thesis. This thesis also investigated the nature, prevalence, severity, and natural history of cannabis-related problems among a heterogeneous sample of 212 ‘at risk’ adolescent and adult users recruited from the community. In a 3-phase developmental design the CUPIT question candidates were first generated employing an Expert Panels methodology. The CUPIT structure, crosssectional, and longitudinal psychometric properties were then systematically tested, incorporating measures of cannabis-related pathology and DSM-IV/ICD-10 diagnoses of cannabis use disorders as criterion standard. High levels of cannabis consumption and related health and psychosocial problems reported portrayed a highly-disordered sample, most marked among adolescents. DSMIV/ICD-10 diagnoses were almost universal with no significant adolescent/adult differences in dependence symptoms count or severity. The two CUPIT subscales (Impaired Control, Problems) derived from principal components analyses exhibited good test-retest and internal consistency reliability and highly significant ability to discriminate diagnostic subgroups along the severity continuum (nonproblematic, risky, problematic use). At the 12-month follow-up, 194 adolescents and adults reported significantly increased cannabis consumption (adolescents), symptoms, and dependence severity. Baseline CUPIT subscale scores demonstrated highly significant longitudinal predictive utility for respondents’ diagnostic group membership, health and psychosocial problems, and significantly improved prediction of other measured outcomes in conjunction with age and gender. ROC analyses identified a CUPIT score of 12 to be the optimal cut-point for maximum sensitivity for both currently diagnosable cannabis use disorder and those ‘at risk’ in this sample. The empirical findings of this thesis research provide a compelling rationale for systematic implementation of opportunistic SEI among consumers of publicly-funded health and social services in New Zealand. Data confirmed that the vast majority of those needing help are unaware, or do not perceive, they need help. This thesis argues that, facilitated by the CUPIT, reliable proactive detection and appropriate intervention for early-stage cannabis use problems has potential for enormous cumulative impact on public health gains and the individual’s quality of life.