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1
Kara, Abdulvahap. "Türkistan ateşi Mustafa Çokay'ın hayatı ve mücadelesi /". Harbiye, İstanbul : Da Yayıncılık, 2002. http://catalog.hathitrust.org/api/volumes/oclc/52135762.html.
Testo completo
2
Gürdal, Muhterem Nur Oğurlu İdris. "Türkiye'nin yaban hayatı koruma ve geliştirme sahaları üzerine araştırmalar /". Isparta : SDÜ Fen Bilimleri Enstitüsü, 2008. http://tez.sdu.edu.tr/Tezler/TF01159.pdf.
Testo completo
3
Hayati, Ashtiani Karim. "Les relations littéraires entre la France et la Perse de 1829 à 1897". Lyon 2, 2004. http://theses.univ-lyon2.fr/sdx/theses/lyon2/2004/hayati-ashtiani_k.
Testo completoAbstract (sommario):
Pour analyser au mieux l'influence de la littérature persane sur la littérature française, après une introduction historique, nous avons examiné un certain nombre de points qui nous paraissaient significatifs : Chap. I, les caractères de la traduction d'Omar Xayyâm par J. B. Nicolas (1867), Chap. II, la présence de la littérature persane dans l'oeuvre poétique de Victor Hugo, Chap. III, le cas Jean Lahor, Chap. IV, le cas Judith Gautier et un aperçu de l'influence de Medjnoun et Lei͏̈la sur l'oeuvre de Gobineau et Leconte de Lisle, Chap. V, André Gide et la Perse. Dans les deux appendices qui ont suivi : 1. Nous avons fourni au lecteur une série d'indications sur les auteurs persans qui ont été portés plus ou moins à la connaissance du public français (traductions de leurs oeuvres ; indications sur leurs caractéristiques). Nous trouvons également un résumé de différents genres de la poésie persane. 2. Nous avons établi deux bibliographies développées des relations entre la Perse et la France de 1829 à 1897.
4
Hayati, Ashtiani Karim Pich Edgard. "Les relations littéraires entre la France et la Perse de 1829 à 1897". Lyon : Université Lumière Lyon 2, 2004. http://theses.univ-lyon2.fr/sdx/theses/lyon2/2004/hayati-ashtiani_k.
Testo completo
5
Islam, Abu Hayat Md Saiful [Verfasser]. "Impact of Technological Innovation on the Poor / Abu Hayat Md. Saiful Islam". Frankfurt : Peter Lang GmbH, Internationaler Verlag der Wissenschaften, 2016. http://d-nb.info/1090772890/34.
Testo completo
6
Nan, Chia-ho, e 南珈合. "Embryology in Cinnamomum kanehirae Hayata". Thesis, 2009. http://ndltd.ncl.edu.tw/handle/48688780994752651928.
Testo completoAbstract (sommario):
碩士臺灣大學
森林環境暨資源學研究所
98
Cinnamomum kanehirae Hayata is an endemic species to Taiwan. It is a large evergreen tree. Hermaphrodite flowers are produced from January to March, and drupe mature during September to October. Inflorescence primordia occur in Mid-December. Anther primordia appear by late December. Anthers are 4-celled and open by uplifting valves. The anther walls of valves are five (to six) layers consisting of an epidermis, an endothecium, two (to three) middle layers, and a tapetum. The tapetum is of amoeboid type. Tapetal cells have up to four nuclei. Microspore mother cells undergo meiosis through successive cytokinesis from mid to late January and give rise to tetrads of microspores, which are mostly isobilaterally arranged and seldom tetrahedrally or in “T” shap. Anthers are dehiscent in early February. Mature pollen grains are 2-celled in early February, and they are spheroidal and inaperturate. Carpel primordia appear in early January. Ovule primordia appear in mid- January. Ovules are anatropous, bitegmic, crassinucellate and with 2~5 cell-layered nucellar cap while embryo sac is mature. From mid to late January, the outer integument is of 3~5 layers of cells in thickness, while the inner integument is composed of 2~3 layers of cells. The micropyle is formed by inner integument. A megaspore mother cell undergoes meiosis, resulting in a linear tetrad of megaspores. The megaspore at chalazal end is functional. The development of embryo sac is of the Polygonum type. Three antipodal cells degenerate soon before fertilization. Developing endosperm is of the nucellar type. The embryogeny is of the Onagrad type. Mature seed exalbumious; cotyledons straight, auricled and massive.
7
WANG, JIAN-ZHEN, e 王健珍. "Immunomodulators from paris formosana hayata (Liliaceae)". Thesis, 1987. http://ndltd.ncl.edu.tw/handle/80897727091390782880.
Testo completo
8
Wen, Mei-Chuan, e 溫美娟. "Population Genetics of Rhododendron rubropilosum Hayata var. taiwanalpinum and Allelopathy of Rhododendron pseudochrysanthum Hayata at Mt. Hohuan". Thesis, 2009. http://ndltd.ncl.edu.tw/handle/94514933178097920573.
Testo completoAbstract (sommario):
碩士中國醫藥大學
生態暨演化生物學研究所
97
Rhododendron rubropilosum Hayata var. taiwanalpinum, one of the endemic Rhododendron species, distributes in high mountain area of Taiwan. According to the literature review, little information on genetic structure and its relationship with climate changes was reported. In this study, the genetic structure of R. rubropilosum taiwanalpinum in relation to climate changes was examined based on microsatellite loci. The result indicated that the isolated eight microsatellite loci were significantly deviated from Hardy–Weinberg expectations due to the heterozygote deficiency which showed the loss of genetic polymorphisms. The FST of R. rubropilosum taiwanalpinum was 0.0532 on average, showing lower differentiation. The analysis of the relationship between genetic diversity and population size revealed that the vulnerability of population was correlated to the population size and low diversity in the higher altitude of this area. The findings suggested that the genetic diversity of R. rubropilosum taiwanalpinum was positively correlated to population size as affected by temperature, and also correlated to the altitude of its distribution. Rhododendron pseudochrysanthum Hayata, a dominant vegetation, is almost lacking of understory species along the trail of East Peak of Mt. Hohuan. Based on our observation, we thus hypothesized that the unique phenomenon might be due to the allelopathy. To test the hypothesis, bioassays of the 1% to 5% aqueous extracts of leaves of R. pseudochrysanthum showed significant inhibition on radicle growth of Brassica chinensis, Lactuca sativa, Amaranthus gangeticus and Bidens pilosa. Because plant residues of the Rhododendron may release allelopathic compounds to suppress the growth of understory species during the decomposing plant residues in soil, we thus conducted a serious of experiment by using 50 g leaves soaked in 950 ml distilled water and allowed the leaves to be decomposed in different time intervals, 1, 2, 4, 8 weeks. The aqueous extracts were obtained at the elapsed intervals of decomposition, and were bioassayed. The bioassay results of the aqueous extracts showed significant inhibition on radicle growth of B. chinensis, L. sativa, A. gangeticus and B. pilosa. We also found the most inhibition was shown in the first and second aqueous extracts, but the phytotoxicity decreased in the subsequent extracts. Furthermore, phytotoxic phenolic acids, such as o-hydroxyphenylacetic, vanillic acid, p-coumaric acid, syringic acid, p-hydroxybenzoic acid, 3,4,5-trimethoxybenzoic acid, veratric acid, gentisic acid, protocatechuic acid, ferulic acid, o-coumaric acid and caffeic acid were tentatively identified as compared with authentic compounds by means of paper chromatography. The amount of these compounds was decreasing with time interval of decomposition, indicating that the phytotoxicity decrease was correlated to the amount of phytotoxic phenolics present in the extracts. Regardless of the adaptation of Rhododendron plants to the climate and environment, the existence of allelochemicals might be responsible for the exclusion of plant species underneath Rhododendron stands, resulting in the low plant diversity of understory species.
9
Yu, Ting-Feng, e 余鼎峯. "Study of Behavior of Devil Tanoak Tree ( Lithocarpus lepidocarus (Hayata) Hayata ) Shoot, Flower and Fruit Growth and Development". Thesis, 2013. http://ndltd.ncl.edu.tw/handle/15538801359124817086.
Testo completoAbstract (sommario):
碩士國立臺灣大學
園藝學研究所
102
This article is divided into three sections: the shoot development , fruit ecology and development, and preliminary breeding of the devil tanoak (Lithocarpus lepidocrpus (Hayata) Hayata). The first section is the development of the devil tanoak shoot. Devil tanoak of high altitude observation point is distributed about an altitude of 1500 meters and located at road sides of Lixing industrial road in Nantou County, with the total count of 15 observation trees. Devail tanoak of low altitude observation point is growing at about an altitude of 70 meters in Wufeng, Taichung, and total observation trees are five 7-9 year old trees. The sprouting period of the devil tanoak is usually in March to April. After the shoots are mature, inflorescence and anthesis form a number of top buds appears in August to September. Therefore, the devil tanoak of high altitude grows vegetative shoots and inflorescence one time in a year. The devil tanoak of low altitude is sprouting in March and July. Each shoots is almost inflorescence after mature. May and October are more obvious flowering and the fruit also mature at December to next year’s January and next year''s September. The second section is the fruit ecology and development of devil tanoak. The devil tanoak of high altitude usually completely finishes anthesis in October. And pistillate flower will be little fruit stage to pass winter and into the period of fruit stagnation. Until next year''s new shoots inflorescence, the fruit will significantly grow. The devil tanoak of low altitude has two anthesis period. Spring fruit is the fruit from the shoot first sprouted in March and finished anthesis, and the curve of growth from fruit diameter, height, weight are fitted S curve. The fruit will be mature in December to next year’s January and natural drop from shoots. Autumn fruit is the fruit from the second sprouted in July. The curve of growth from fruit diameter, height, weight is fitted double S curve. Autumn fruit should start growing until next year''s Spring flower anthesis, and the fruit will be began to be mature in October. Therefore, the fruit from Spring or Autumn flower grow in significant difference. The third section is the survey of calibration tanoak fruits and seed traits and preliminary breeding. The experiment method is picking up 30 to 40 fruits per plant, and removing the bad fruit, for example, bitten by pests, and then randomly selected 20 fruits per plant. Record the fruit and seed traits. Due to the cupule of natural drop fruit integrity are greatly diverse, so we removed the cupule of fruit and measured the content which only contains shell''s nut weight, nut height, nut diameter, and the edible seed weight without shell. After analysis, we found seed weight is significantly correlated with nut weight, nut height, and nut diameter. And we will found nut weight is the most important factor. Also, high edible rate is an important factor that we need. Therefore, we can also perform preliminary breeding based on the tree''s trait of nutweigt:seedweight''s ratio. Among the selection of the current investigated plants, LC201 and LC197 both shows high rate and high edible weight, and thus could be the best strains.
10
Cheng, Yu-Pin, e 鄭育斌. "The phylogeographical study of Castanopsis carlesii Hayata". Thesis, 2006. http://ndltd.ncl.edu.tw/handle/60349140968989555863.
Testo completoAbstract (sommario):
博士國立臺灣大學
植物科學研究所
94
The noncoding region sequence of cpDNA and the allozyme marker were used to conduct the phylogeographical study of Castanopsis carlesii, a subtropical and temperate tree species in Taiwan. C. carlesii, belonging to the Fagaceae, is one of the most common and dominant tree species which adapt to the subtropical and tempetate climate in Taiwan. In this study, we examined spatial patterns of chloroplast DNA (cpDNA) variation in a total of 30 populations of C. carlesii, including 201 individuals sampled throughout Taiwan. By sequencing two cpDNA fragments using universal primers (the trnL intron and the trnV-trnM intergenic spacer), we found a total of 1663 bp and 21 polymorphic sites. These gave rise to a total of 28 cpDNA haplotypes. The level of differentiation among the populations studied was relatively high (GST = 0.723). Two ancestral haplotypes are widely distributed. The Central Mountain Ridge (CMR) of Taiwan represents an insurmountable barrier to the east-west gene flow of C. carlesii. Among the populations studied, three separated populations, at Lienhuachih, Fushan and Lichia, have high nucleotide diversity. Estimates of NST - GST for populations on both sides of the CMR indicate that no phylogeographical structure exists. According to the genealogical tree, number of rare haplotype and population genetic divergence, this study suggests that two potential refugia existed during the last glaciation: the first refugium was located in a region to the north of Hsuehshan Range (HR) and west of the CMR; the second refugium was located in south, especially southeastern Taiwan. In fact, the second refugium happens to be the same as that reported for Cyclobalanopsis glauca. A “Star-like” genealogy is characteristic when all haplotypes rapidly coalesce and is a general outcome of population expansion. The neutrality test and mismatch distribution also suggest demographic expansion. Using genetic parameters to determine the heterozygosity and the mean FST value of each population, we inferred the evolutionary history of Castanopsis carlesii in Taiwan. In this study, 22 populations of C. carlesii throughout the island were sampled covering an elevational range of 50~2300 m. Starch gel electrophoresis was used to assay allozyme variations, and 11 loci from nine enzyme systems were detected. Average values of genetic parameters describing the within-population variation, the average number of alleles per locus (A = 2.5), the effective number of alleles per locus (Ae = 1.38), the allelic richness (Ar = 2.38), the percentage of polymorphic loci (P = 69%), and the expected heterozygosity (He = 0.270) were estimated. High levels of genetic diversity were found for C. carlesii compared with other local plant species. From the data of expected heterozygosity, one major diversity center was situated in central Taiwan corroborating previous reports for other plant species. According to the mean FST value of each population against the remaining populations, the most-divergent populations were situated in two places. One includes the populations of Anmashan, Fushan, and Tungao, and is located in north-central Taiwan between 24.80°N and 24.20°N. The other is located in southeastern Taiwan between 22.40°N and 23.10°N, and includes the populations of Hsinkangshan, Tona, and Tawu. These two regions are approximately convergent with the most divergent locations determined for several other plant species using chloroplastic DNA markers. In conclusion, the result of genetic differentiation study obtained from isozymes agrees well with that from chloroplastic DNA markers.
11
Jui-Lin, Chang, e 張瑞麟. "The Genetic Diversity of Taiwania cryptomerioides Hayata". Thesis, 2005. http://ndltd.ncl.edu.tw/handle/13267387275940089680.
Testo completoAbstract (sommario):
碩士國立中山大學
生物科學系研究所
93
The aim of this study was to obtain the molecular marker of Taiwania cryptomerioides Hayata based on DNA sequence data of PCR- sequencing and inter-simple sequence repeat (ISSR), and to evaluate the genetic diversity of populations of Taiwania cryptomerioides Hayata and molecular phylogeny of T. cryptomerioides and Taiwania flousiana Gaussen. The sequence data based on the internal transcribed spacer (ITS) of a total of 108 samples of T. cryptomerioides were determined. Eight different populations of T. cryptomerioides and 12 samples of T. flousiana from Yunnan, China were analyzed. The finding of the study showed that heterogeneity of ITS region within individuals of T. cryptomerioides was high by showing high nucleotide diversity among ITS sequences both in T. cryptomerioides ( π = 0.18153) and T. flousiana ( π = 0.19751). The findings fit in Tajima’s D test of neutrality based on DNA sequence variation in the ITS region of T. cryptomerioides and T. flousiana. It is not obvious to incorporate into different population through clustering analysis based on data of the ITS region of T. cryptomerioides and T. flousiana. However, slightly genetic differentiation between T. cryptomerioides and T. flousiana was found, which figured of Fst (Fst = 0.0441~ 0.0856, an average value = 0.0611). On the other hand, the samples were studied by using ISSR markers. Of the 100 primers screened, 4 produced highly reproducible ISSR bands, and 24 discernible DNA fragments were generated with 17 being polymorphic. Based on cluster analysis of molecular data, the cluster is not clear among populations of T. cryptomerioides and T. flousiana. The analysis of AMOVA revealed that the variance component between species of T. cryptomerioides and T. flousiana was 38.54％ (P < 0.001); however, the variance component within species is 61.46 (P < 0.001). The variation within population of T. cryptomerioides was 84.74％ (P < 0.001) and the variance between populations is 15.26％ (P < 0.001), indicating that the genetic diversity of individuals within population was high. The aforementioned data suggest that gene flow among different populations of T. cryptomerioides was high, indicating that the genetic diversity was high among individuals of T. cryptomerioides but was low between populations. Furthermore, it is concluded both species are genetically closer and could be grouped into the same species.
12
Huang, Wei-Jie, e 黃偉傑. "Sexual System of Daphne arisanensis Hayata (Thymelaeaceae)". Thesis, 2017. http://ndltd.ncl.edu.tw/handle/4rbsqx.
Testo completoAbstract (sommario):
碩士國立臺灣大學
生態學與演化生物學研究所
105
Angiosperms exhibit great diversity of sexual systems, as the majority of extant species are hermaphrodite, the evolution of unisexuality has drawn attention since Darwin’s age. The reproductive biology of Daphne arisanensis Hayata (Thymelaeaceae), an endemic shrub in Taiwan, had rarely been studied before. Field observations implied that populations of D. arisanensis comprise morphologically bisexual and male-sterile individuals, hence this species may be gynodioecious. However, no unequivocal evidence had been presented yet. To clarify the true sexual system of D. arisanensis, I investigated the spatial and temporal expression of sexuality, and tested the reproductive potential of each floral morph. In this study, phenology surveys were conducted in Meifeng, Yuanfeng, Hehuanshan, Dayuling and Guanyuan during 2014-2017. Pollination experiments were operated in Guanyuan and the Hehuanshan-nearby alpine populations respectively. No individual altered the flowering type in this three-year observation. Male-sterile flowers had the ability to set fruits either with pollination or without fertilization (i.e. apomixis). The gynoecia of morphologically bisexual flowers displayed receptivity to pollens from either itself or other plants, but they hardly set fruits among all pollination treatments. In addition, unusual flowers with different degrees of male-sterility were occasionally found on morphologically bisexual plants. The extremes were quite similar to the typical “female” flowers in male-sterile plants and indeed set fruit under open pollination. The seeds from all kinds of treatments showed germination potential. Great variations in morph ratio, floral traits and floral phenology were found among populations. Compared to the Guanyuan population, individuals in alpine areas showed larger flower size, longer floral lifespan and lower fruit-set rates under both open pollination and apomixis. Various nonspecific nocturnal moths were observed pollinating in both of the populations. To provide materials for inferring the possible evolutionary scenario of the gender divergence in D. arisanensis, I also conducted bagging experiments to another congeneric hermaphroditic species, D. kiusiana Miq. var. atrocaulis (Rehder) Maekawa, in Yangmingshan. The results show that 49% of the bagged flowers had set fruits via autonomous selfing, suggesting that self-pollination may be common in genus Daphne. These findings indicate that the sexual system of D. arisanensis is cryptic and leaky dioecy. The flowering phenology and floral traits varied among populations, and the evolution toward dioecism would probably be correlated with the self-pollination pressure in genus Daphne.
13
Wang, Shu-Chun, e 王淑君. "Studies on Antioxidative Enzymes in Anoectochilus formosanus Hayata". Thesis, 2000. http://ndltd.ncl.edu.tw/handle/63391426696380558072.
Testo completoAbstract (sommario):
碩士國立臺灣大學
藥學研究所
88
Abstract： Superoxide dismutase（SOD），a family of metallo-enzymes, catalyze the dismutation of harmful superoxide free radical（O2·-）to molecular oxygen and hydrogen peroxide（H2O2）, and is considered a major enzymatic defense against O2·-. SOD could be classified into three types according to their metal cofactor：CuZnSOD, MnSOD, and FeSOD. The biological activities of the plant, Anoectochilus formosanus in the use of cardiovascular disease and hepatitis may be related to the antioxidants which have never been reported from A. formosanus. In this study, the SOD activity was assayed by combining polyacrylamide gel electrophoresis and densitometry. It was found that the optimal SOD activity of the plant was pH 6.8-7.2 and optimal temperature at 40 °C. Total antioxidant activity（TAA）was determined by HRP＊/ ABST＊ / H2O2 method, and it was found 146.5-176.9 mg ascorbic acid / fresh weight in the plant. In general, the SOD activity and TAA of old leaves of the plant were reduced as compared with young leaves（10﹪for the SOD activity and 15﹪for the TAA）. Based on the sensitivity to H2O2 and KCN, 1 MnSOD and 1-4 CuZnSODs were identified in various plant strains. Therefore, SOD patterns could be used as markers and provide a rapid and easy tool for identification of A. formosanus strains. On the other hand, CuZnSODI was found to be a fast move rate enzyme which has never been found in other plants in our laboratory before. It is considered suitable for purification and determination of amino acid sequence and was purified by ammonium sulfate fractionation, DEAE＊anion-exchange chromatography and FPLC＊ gel-filtration chromatography. The molecular weight of CuZnSODI was found approximately 20.1 KDa as indicated by SDS-PAGE＊ and silver staining. The N-terminal amino acid sequence analysis of CuZnSODI gave 3KAVAVLKGNS12 and identical with the CuZnSOD in Lycopersicon esculentum, Medicago sativa and Marchantia paleacea. ＊ HRP: horseradish peroxidase; ABTS: [2, 2''-azino-bis（3-ethylbenzothiazoline-6-sulphonic acid）]; DEAE: diethylaminoethyl; FPLC: fast performance liquid chromatography; SDS-PAGE: sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Key words： Anoectochilus formosanus, antioxidative enzymes, superoxide dismutase
14
Chen, Yeng-Yi, e 陳穎宜. "Embryology in Machilus japonica var. kusanoi (Hayata)Liao". Thesis, 2003. http://ndltd.ncl.edu.tw/handle/28862142895137221472.
Testo completoAbstract (sommario):
碩士國立臺灣大學
森林學研究所
91
Summary Machilus japonica var. kusanoi is an endemic species to Taiwan﹒ The large evergreen trees are monoecious﹒Bisexual flowers are produced from January to March﹒ Fruit maturing June to July﹒ Inflorescence primordia are emerged from the apical meristem in early- January. Anther primordia are composed of the protoderm and the central cell mass in mid- January. The protoderm will differentiate into the epidermis later. As pollen mother cells are formed, the wall layer is consisting of, from inner to ourter, a tapetum cells, two middle layers, an endothecium, and the epidermis. Isobilateral tetrads of microspores are mostly formed by successive cytokinesis. Mature pollen grains are 30~45μm ×30~45μ in size, 2-celled. Anther dehiscening with valves. Ovule primordia are formed on the enrolled carpel margins in mid- January. The megaspore mother cell is located at the 5rd to 7th layer of cells in the nucellus. It divides transversally to form a linear megaspore tetrad, and the functioning megaspore at chalazal end. The embryo sac is monosporic and follows to the 8-nucleate Polygonum type. The egg cell and two synergids are located at the micropylar pole of the embryo sac. Two polar nuclei fuse before fertilization. Three antipodal cells are ephemeral. The endosperm consists of free nuclei during development. The embryogeny is identified to the Asterad type. Each seed contains 2 massive cotyledons.
15
Weng, Li-Chien, e 翁儷倩. "Genetic variance among tankan ( Citrus tankan Hayata ) accessions". Thesis, 2001. http://ndltd.ncl.edu.tw/handle/27740606983199600243.
Testo completoAbstract (sommario):
碩士國立臺灣大學
園藝學研究所
89
The tankan accessions witch selected by fruit size and brix scale were collected from Hsinchu, Taichung and Nantou. For further analysis, they were grafted on ‘sunki’ mandarin and planted at Horticultural Station. The objective of this study was to identify the genetic variance among the tankan accessions and the genetic relationships among tankan and other citrus. The unstability fruit characters of several accessions suggusted that they might still be in juvenile phase. Therefore, only vegetative characters and RAPD markers were employed in the analysis. The vegetative characters investigated included shoot length, node number, leaf length, leaf width, leaf length/leaf width, wing length, wing width, wing length/wing width, leaf length/wing length, leaf thickness, leaf margin length, margin serration number, margin serration number/cm, oil gland number/cm, oil gland number/cm2, and leaf area. Through cluster analysis of these 16 characters, the tankan accessions diverted into two main groups, big and small fruit group respectively. Within each group, the accessions displayed diversity as well. The grouping patterns of the accessions showed no relevance to geographical origin. The ordination pattern of the principal component analysis was similar to the grouping pattern of the cluster analysis, and the first three principal components derived from the principal component analysis explained 76.28% of the observed variation. According to principal component analysis, leaf length/leaf width, wing length, margin serration number/cm, oil gland number/cm and oil gland number/cm2 were the major discriminating traits. The dendrogram generated by cluster analysis based on the 5 major discriminating traits was resemblance to that based on 16 vegetative characters. Therefore, our result indicated that the 5 major discriminating traits could be useful for tankan genetic variance analysis and for early selection of fruit size. 293 10-mer random primers were screened in the RAPD analysis, 28 of which produced no amplification, and 250 of which amplified monomorphic products, and 15 of which produced polymorphism. The monomorphic bands produced by the 15 primers were reproducible; however, the polymorphic bands were not reproducible. The 265 primers generated total 1517 reproducible monomorphic bands revealed high genetic similarity among the tankan accessions. The non-reproducibility of the polymorphic bands might suggest the complexity of the genetic nature of tankan. Whereas, the PCR condition caused these perplexed result could not be ruled out. Principal component analysis of 16 vegetative characters was also carried out to establish the relationship of tankan and other citrus. The first three principal components accounted for 84.69% of the total variance. The ordination of tankan and other citrus after principal component analysis was similar to the grouping patterns generated by cluster analysis. Mandarins and tankan were clustered in a group, and sweet oranges, grapefruits and minneola tangelo were clustered in another. 19 chosen primers from 250 primers screened in the previous work produced 189 stable bands. Among the 189 bands, 153 were polymorphic. The dendrogram generated by cluster analysis based on 153 polymorphic bands was resemblance to that based on 16 vegetative characters. These results indicated that tankan was closer to mandarins than to sweet oranges, grapefruits and minneola tangelo and didn’t support that tankan is one of the subsection Sinensioides. Besides, the highly similarity among haili and tankan supported the possibility that haili may derived from tankan.
16
Chen, Ting-Ling, e 陳亭伶. "Antioxidation and anticogulant activity of Pinus morrisonicola Hayata". Thesis, 2016. http://ndltd.ncl.edu.tw/handle/19618047722933634186.
Testo completoAbstract (sommario):
碩士國立屏東科技大學
食品科學系所
104
Many literatures reported that phenolic compounds is known to exhibit antioxidant and antithrombogenic effects and reduce the risk of cardiovascular diseases. Key point of the development of cardiovascular disease is according to vascular smooth muscle cell proliferation. The present study was to investigate the regulation of blood pressure by Pinus morrisonicola Hayata. Primarily, test samples were prepared by extraction in water, ethanol and ethyl acetate solvents or fermented by various sample/sugar ratio during 12 weeks periods. Determination of antioxidant activity, including reducing power, DPPH radical scavenging activity and ABTS+ radical cation scavenging assays were conducted. Furthermore, total phenolic contents, flavonoid content and condensed tannins content were investigated. The results demonstrated that different treatment of DPPH radical scavenging capacity was between in 60-80%, ABTS+ radical cation scavenging rate was about 60% and reducing power reached up to 0.85 times of BHA equivalent. It showed good efficacy of inhibitory rate. The condensed tannins content suggest a time-dependent relationship. Total phenolic content of Pinus morrisonicola Hayata is 500-800 μg GAE / ml at 12th week. And flavonoids contents is 10-18 μg quercetin /ml at 12th week fermentation. The phenolic compounds and flavonoids in extracts are higher than those in ferments. Both of extraction and fermentation, illustrated that active ingredients analyzed by HPLC, abietic acid and β-sitosterol, are 91-243 ppm and 66-760 ppm observed respectively. Conclusively, our data showed the optimal fermentation period was three to four weeks. The concentration and antioxidant capacity of sample extract was positively correlated. Both extracts and ferments of Pinus morrisonicola Hayata could inhibit smooth muscle cell growth about 20-30%, and can effectively extend the activated partial thromboplastin time (APTT) more than 30 seconds, showing anticoagulant activity. Obviously, the development of health drink from Pinus morrisonicola Hayata is expected in the near future.
17
Wang, Shao-Ning, e 王紹嬣. "Studies on Harvest Maturity and Storability of Tankan (Citrus tankan Hayata) Fruits.Studies on Harvest Maturity and Storability of Tankan (Citrus tankan Hayata) Fruits.Studies on Harvest Maturity and Storability of Tankan (Citrus tankan Hayata) Fruits". Thesis, 2008. http://ndltd.ncl.edu.tw/handle/66799917717200082330.
Testo completoAbstract (sommario):
碩士國立宜蘭大學
園藝學系碩士班
96
The goal of this experiment is to investigate the most suitable harvest maturity, increase storability and discuss the possibility of delaying harvest and the influence of different pre- and post -harvest processing method to the tankan quality and storability. Increase fruit appearance and fruit storage quality to create higher output value. The fruit of different harvest date, with the harvest maturity increase, the peel L value lower, the peel getting darker. The peel color a value rise, peel color from green to red with the harvest date delay. With the harvest maturity increase, the peel b value decrease, peel color from yellow to orange. The excessively late harvest one can increase the fruit peel thickness; the TSS and TSS/TA can increase, but titrate acid getting lower. Fruit after harvest which spray by 3% calcium chloride and dips in 53 ℃ hot water for 3 minutes, then separate stores it at 5 ℃ and 15 ℃, the 15 ℃ group have highest decay rates. 12 weeks later, both heat treatment and calcium chloride processing group are completely rotten. But after 12 weeks, 5 ℃ group’s heat treatment have lowest decay rate, the calcium chloride is next, untreated fruit have highest decay rate. There’s no difference in weight loss rate, the peel color, fruit pulp degree of hardness and the fruit juice percentage when stores in 15 ℃ no matter what treatment have been done. But fruit of every treatment after storage in 5 and 15 ℃, the TSS and TSS/TA are both getting higher with the time pass, but the TA is going opposite way. Compare 1.5 and 3% pre-harvest calcium chloride treatment,1.5% treatment group has brightest peel color and highest fruit juice percentage. All three treatment have no affect in fruit size, peel thickness, the TSS, TA, and TSS/TA. No matter when doing the pre-harvest bagging, always have higher peel color L, a and b value then unbagging fruit. It means bagging fruit are brighter and more orange. Early bagging can raise fresh weight, but late bagging shows otherwise. The juice percentage between different bags are between 45.6-50.5％, no specific difference between them. The peel thickness is higher after bagging compare to control group, there’s obvious differences. Generally bagging will lower the TSS of the fruit, the earlier the TA lower, and earlier the TSS/TA higher.
18
高淑芳. "Study on the chemical constituents of Rosa transmorrisonensis Hayata". Thesis, 1992. http://ndltd.ncl.edu.tw/handle/63060494901728419656.
Testo completo
19
Kuo, Fu-Lin, e 郭福麟. "tudy on population genetic structure of Hypericum nagasawae Hayata". Thesis, 2009. http://ndltd.ncl.edu.tw/handle/68skt2.
Testo completoAbstract (sommario):
碩士國立臺灣師範大學
生命科學研究所
97
Hypericum nagasawae Hayata and H. nokoense Ohwi are both endemic species in Taiwan. It is difficult to determine these two species since the closely morphological similarity between them. In this study, 4 chloroplast DNA non-coding regions, accD – psaI intergenic spacer, trnE – trnT IGS, rpl16 intron, and trnV – trnM (trnV intron included) IGS, were used to analyze their relationship. The results of molecular analysis are consistent with the taxonomic treatment of Robson (1996). Geographically, H. nagasawae is widely distributed in mountainous regions above 2,300 m in Taiwan, while H. nokoense is restrictively distributed in limestone regions in Shiu-Lin Township, Hualien. In order to uncover the population genetic structure of H. nagasawae, a total of 150 samples were collected from 10 populations, and two chloroplast DNA intergenic spacers, accD – psaI and trnE – trnT, were analyzed. 10 haplotypes were detected with 4 main haplotypes limitedly distributed in specific regions. Genetic differentiation among populations was generally high (mean FST = 0.744, Nm = 0.09). Both haplotypes in Mt Peitawu are endemic with the farthest genetic distance from other haplotypes. It suggested that the populations between Mt Peitawu and other regions have a long-term geographical isolation. The genetical distance and geographical distance (Isolation by distance test) have a moderate positive correlation when the population of Mt Peitawu was excluded. According to the result of Nested Clade Analysis, past fragmentation followed by range expansion had occurred. By integrating the present result and previous studies for other alpine plants, Northern Central Ridge was the genetic diversity center of alpine herbs of Taiwan. Glaciation might have played an important role contributed to the distribution pattern of haplotypes. According to neutral theory test (Tajima’s D, Fu & Li’s test) and BEAST software, however, last Glacial Age did not significantly influence population size.
20
Liu, Chin-Cheng, e 劉金城. "Studies on the Bioactive Lignans of Schisandra arisanensis Hayata". Thesis, 2008. http://ndltd.ncl.edu.tw/handle/75936919739614013887.
Testo completoAbstract (sommario):
碩士國立臺灣大學
藥學研究所
96
This research focuses on lignans from Taiwanese medicinal herb Schisandra arisanensis Hayata. Their CH2Cl2 and MeOH extracts were investigated by using chromatography and twenty lignans were isolated. In the course of our study on lignans of S. arisanensis, five new compounds, arisanschinin A (1), arisanschinin B (2), arisanschinin C (3), arisanschinin D (4), arisanschinin E (5) together with fifteen known compounds, binankasurin A (6), kadsurarin (7), schisantherin B (8), schisantherin A (9), meso-dihydroguaiaretic acid (10), kadsumarin (11), kadsulignan K (12), kadsulignan D (13), kadsurin (14), angeloyl-binankadsurin A (15), acetyl-binankadsurarin A (16), schizanrin F (17), schisantherin D (18), kadsuphilol A (19) and kadsuphilol C (20) were isolated by using liquid chromatography. The new compounds 1-4 possess a C18 dibenzocyclooctadiene skeleton and compound 5 has an open ring system, biphenyldimethylbutane. All the structures of above compounds were elucidated by 1D, 2D-NMR spectroscopic analysis and physical methods including IR, mass, UV, optical rotation and NMR, and also by comparisions with the published data. The absolute stereochemistry of compounds 1-5 was determined by circular dichroism and NOESY. According to the spectra of CD, the configuration of compounds 1-2 were R form and compounds 3-4 were S form. The radical-scavenging activity of these compounds were tested and evaluated by reation with DPPH (1, 1-diphenyl-2- picryhydrazyl) free radical.
21
Wu, Wan Yu, e 吳宛諭. "Studies on the Chemical Constituents of Dendrobium sanseiense HAYATA". Thesis, 2004. http://ndltd.ncl.edu.tw/handle/23718859938387066173.
Testo completoAbstract (sommario):
碩士中國醫藥大學
中國藥學研究所
92
This study was based on the investigations and literature survey of phytochemistry, polysaccharide, fungus of Dendrobium genus. This research provided some informations for chemical studies of Shu-hu . In the course of our studies on the constituents of Taiwanese Orchidaceae plants, this study carried out the chemical analysis of Dendrobium sanseiense HAYATA. Five compounds were isolated from the the stems of Dendrobium sanseiense HAYATA. On the basis of spectral analysis, they were identified as 2,3,4,7-tetramethoxyphenanthrene, phytosterol, stigmast-4-en-3-one, heptacosane, and hexacosanol. From GC, GC/MS analysis, long chain alkanes, alkenes, fatty acids were also detected in the fractionated hexane extract, respectively.
22
Fang, Hong Ming, e 方宏銘. "Studies on the active constituents of Heterostemma brownii hayata". Thesis, 1995. http://ndltd.ncl.edu.tw/handle/67456588393834838604.
Testo completo
23
Jhan, Yun-Lian, e 詹昀璉. "Chemical constitutes of Eurya glaberrima Hayata and theirbiological activities". Thesis, 2011. http://ndltd.ncl.edu.tw/handle/32133852965463786892.
Testo completoAbstract (sommario):
碩士中國醫藥大學
生態暨演化生物學研究所碩士班
99
In previous studies, some phytochemicals isolated from species of Eurya, a member of Theaceae family, showed various bioactivities. For example, chrysoeriol from methanolic extract of leaves of Eurya ciliata stimulated proliferation and differentiation of osteoblastic MC3T3-E1 cells. E. emarginata is also used as a traditional folk medicine in Korea to treat ulcers and diuretic. E. glaberrima Hayata is one of the endemic species distributed in broad-leaved and mixed forest at elevations between 1500 to 3300 m above sea level in Taiwan. In order to clarify the medical and ecological roles of E. glaberrima, the natural products of E. glaberrima with anti-hepatocarcinoma cell, allelopathic activity were investigated. In this study, we used the methanolic extract of twigs and leaves of E. glaberrima to test the cytotoxicity and isolated natural compounds. On the other hand, we used the aqueous extract of E. glaberrima leaves to test the allelopathic potential. Five known compounds, namely, rengyolone (1), betulinic acid (2), taxifolin 3‒O‒β‒D‒xylopyranoside (3), quercitrin (4) and hyperin (5), were isolated from methanolic extract of E. glaberrima and one acetylated product 3-O-acetyl betulinic (2a) were identified by NMR and ESI-MS-MS data analyses. Rengylone revealed 50 % inhibitory effect at 40 μg/ml on HepG2 2.2.15. 3-O-acetyl betulinic had better cytotoxic effect than betulinic acid. All of them didn’t show the anti-HBV effect. In addition, in order to test the allelopathic potential of E. glaberrima, the aqueous extracts (1~5%) of leaves of E. glaberrima were bioassayed by using six tested plants, namely, Brassica chinensis, Lactuca sativa, Setaria italica, Ocimum basilicum, Cucumis sativus and Bidens pilosa. The results exhibited that the aqueous extracts of E. glaberrima could inhibit the radicle growth but not seed germination of the six tested plants. The leachate of E. glaberrima leaves could significantly inhibit the radicle elongation of B. chinensis and S. italica. In our study, betulinic acid and hyperin didn’t show the allelopathic effect of the tested plants. In conclusion, E. glaberrima maybe has relative low potential for developing as a medicinal plant. The aqueous extracts and leachate of E. glaberrima have the phytotoxic potential, exhibiting suppressive effect on some species underneath the Eurya vegetation in the field.
24
Yeh, Hung-Chun, e 葉宏淳. "Chemical Constituents and Biological Activities of Cinnamomum reticulatum Hayata". Thesis, 2009. http://ndltd.ncl.edu.tw/handle/53526053757679072386.
Testo completoAbstract (sommario):
碩士輔英科技大學
醫事技術系碩士班
98
Cinnamomum reticulatum Hayata （Lauraceae） is an evergreen trees, an arbor indigenous to Taiwan. We have collected the methanolic extracts of the leaves and stems of C. reticulatum Hayata for evaluation of their in vitro antiproliferative effects and antioxidant capacity. Therefore, we investigated the chemical constituent of the leaves and stems of C. reticulatem. Totally 12 compounds including two sesquiterpenoids：tenuifolin (1) and reticuol (2); one normonoterpenoid: reticuone (3); one steroid ： β-sitostenone (4); one benzenoid ： p-hydroxybenzoic acid (5); one monoterpene ： 2,6-dimethyl-1,7- octadiene-3,6-diol (6); one lignan：isoanwulignan (7); one quinone: α-tocopheryl quinone (8); two flavonoids：kaempferol-3-O-(2″,4″-di-E- p-coumaroyl)-α-L-rhamnopyranoside (9) and kaempferol-3-O-(3″,4″- di-E-p-coumaroyl)-α-L-rhamnopyranoside (10); two chlorophylls ： pheophorbide a (11) and aristophyll C (12). Among them, tenuifolin (1), reticuol (2) and reticuone (3) are new compounds. On antiproliferative effects, 10 showed inhibition of A549、 NCI-H460、MCF-7 and MDA-MB231. On antioxidant capacity, 9 showed scavenging effect of DPPH, and 9 and 10 effect of reducing power.
25
Chang, Chao-Lin, e 張肇麟. "The Induction of Somatic Embryo of Picea morrisonicola Hayata". Thesis, 1998. http://ndltd.ncl.edu.tw/handle/26814486277611972748.
Testo completoAbstract (sommario):
碩士國立臺灣大學
森林學系
86
Results of mature embryo culture and somatic embryo induction of Picea morrisonicola Hayata were as follows: mature seeds were first treated with running water for 48 hours, then soaked in 70% ethanol solution for 2 minutes. Soaked in 4% NaOCl (supplemented 1% (v/v) Tween 20) and treated with ultrasonic shaker for 10 minutes. Mature embryos were them isolated from the seed and 10 mature embryos were transferred into 50 ml conical flask containing 20 ml MS solid culture medium. Callus with different phenotype were induced after they were culture under light and dark conditions respectively. Under light conditions, green and compact callus were formed, but yellow and soft callus were formed under dark. After yellow and soft callus were transferred to medium containing 2 ppm 2,4-D and 1 ppm BA, and cultured under dark conditions for 4 weeks, white and sticky embrygenic callus were induced. These callus contained precotyledonary phase somatic embryos with embryonal head and suspensor . Cotyledonary phase somatic embryos with green and short axis were induced after these callus were transferred onto MS solid medium supplemented with 1 ppm 2,4-D,1 ppm NAA and 2 ppm ABA and cultured at light conditions for 2 months.
26
Li, Chan-Hao, e 賴建豪. "The Constituents of the Stem of Cissus pteroclada Hayata". Thesis, 2009. http://ndltd.ncl.edu.tw/handle/27558946830059166600.
Testo completoAbstract (sommario):
碩士南台科技大學
生物科技系
97
Fifty-one compounds were isolated from the fresh stem of Cissus pteroclada Hayata. Among these isolates, forty-one compounds were elucidated as β-sitostenone and stigmasta-4,22-dien-3-one mixtures, β-sitosterol and stigmasterol mixtures, 6α-hydroxy-β-sitostenone, 6β-hydroxy-β-sitostenone, palmitoyl glucosyl sitosterol and palmitoyl glucosyl stigmasterol mixtures, oleanolic acid and ursolic acid mixtures, 23-hydroxyursolic acid and hederagenin mixtures, taraxerone, taraxerol, 11α,12α-epoxy-14-taraxeren-3-one, betulinic acid, lupenone, phytal and dihydrophytal mixtures, methyl vanillate, p-hydroxybenzoic acid, p-hydroxybenzaldehyde, methyl-4-hydroxybenzoate, 4-hydroxy-3-methoxyacetophenone, vanillin, ω-hydroxypropioguaiacone, methyl gallate, syringaresinol, 4-ketopinoresinol, methyl pheophorbide-a, methyl pheophorbide-b, methyl-21-hydroxy-(21S)-pheophorbide-a and methyl-21-hydroxy-(21R)-pheophorbide-a mixtures, methyl-21-hydroxy-(21S)-pheophorbide-b and methyl-21-hydroxy-(21R)-pheophorbide-b mixtures, 3,3’-di-O-methylellagic acid, 3,3’,4-tri-O-methylellagic acid, 3,3’,4,4’-tetra-O-methylellagic acid, ellagic acid, bergenin and methyl ester fatty acid.
27
Huang, Tai-Jie, e 黃泰傑. "Robust Design of Light Sources for Cinnamomum kanehira Hayata". Thesis, 2017. http://ndltd.ncl.edu.tw/handle/25902734262310240802.
Testo completoAbstract (sommario):
碩士遠東科技大學
機械工程研究所
105
Cinnamomum kanehirae is an important species of economic tree in Taiwan, but recently, the number of nature population was decreasing gradually. To save those plants, we used tissue planting instead of growing Cinnamomum kanehirae on this endangered tree. However, if we only lit the plants by fluorescent lamps in the progress of tissue culture, the cultivation process is very cumbersome and long cultivation period. The thesis used a dimmable lighting system for R-LEDs, G-LEDs and B-LEDs which provided light source for flask plantlet. The goals were first to find the optimal light combination to obtain the lowest period of cultivation, and second to verify the experiment with big data. Meanwhile, we took advantage of Orthogonal Array- in order to decrease the times of experiments- as a mathematical tool to work the studies. This research will focus on R-LEDs, G-LEDs and B-LEDs, and the on/off periods of these artificial lights. The results showed that the cultivation period of tissue culture plantlets was reduced by 80 days and 43%.
28
SHI, SHU-KAI, e 許舒凱. "Selection and breeding of polyploids in Anoectochilus formosanus Hayata". Thesis, 2016. http://ndltd.ncl.edu.tw/handle/11912885293095689586.
Testo completoAbstract (sommario):
碩士國立高雄大學
生命科學系碩士班
104
The aims of this thesis are selection and breeding of polyploids in Taiwan Jewel orchid, Anoectochilus formosanus Hayata. Plantlets derived from buds of stem nodes that treated with colchicine were used as the materials in this study. Firstly, putative polyploids were obtained through sequentially selections on the morphological characteristics including leaf morphology and plantlet diameter. Subsequently, stomata characteristics of these putative polyploids were analyzed. The parameters were size and frequency of stomata as well as the number of chloroplasts in guard cells. Finally, the ploidy level and chromosome numbers of 2x plants and putative polyploids were determined using flow cytometer. This thesis successfully selected autotetraploids of Taiwan Jewel orchid, which showed significantly higher plant height and diameter with a different leaf shape cordate when compared with leaf shape of 2x plants with ovate. In addition, these autotetraploids had significantly larger stomata size, lower stomata frequency, and higher numbers of chloroplast within guard cells when compared with the 2x plants. The selected polyploids could be used as materials for breeding, medicinal compositions analysis, mass propagation and practical production in the future.
29
Huang, Jian-Tan, e 黃健覃. "Colchicine on polyploidy induction of in vitroAnoectochilus formosanus Hayata". Thesis, 2007. http://ndltd.ncl.edu.tw/handle/66871936593157102615.
Testo completoAbstract (sommario):
碩士中興大學
農藝學系所
95
The objectives of this study were to investigate the effects of colchicine treatments on the polyploidy induction of in vitro nodal and rhizome explants of Anoectochilus formosanus Hayata. First, three anti-microtubule chemicals colchicines, oryzalin, trifluralin at concentration of 25, 100 and 400 μM were added on proliferation medium to treat nodal explants for 2 weeks. Explants were transfered to the acclimatization medium after treatment and data of shoot proliftration and shoot length were collected 4 and 8 weeks after subculturing. The results indicated that all three chemicals inhibited shoot proliferation and growth and among them colchicines was the most effective one. The best polyploidy was induced from 400 μM of colchicines with 67% tetraploids and 33% octoploid, respectively. Various concentrations of colchicine in 0, 0.625, 1.25, 2.5 and 6.25 mM were added into proliferation medium. Nodal explants were treated with colchicine for 3 days before subculturing into an acclimatization medium and the data was collected ten weeks after treatment. The results showed that shoot proliferation, shoot length as well as survival rate of explants decreased along with increasing of colchichine concentrations. The highest percentage of tetraploid was obtained from 2.5 mM colchicine treatment with 80%. Various duration of 0, 3, 6 and 9 days for treatment with 2.5 mM colchicine in proliferation medium was compared. Shoot proliferation rate, shoot length and survival rate of nodal explants were all decrease as the increasing of treatment duration. The highest percentage of tetraploid(50%) was found in the treatment duration of 3 days. Rhizome explants age at 10, 11 and 14 weeks were used for polyploidy induction. Colchicine concentrations of 0, 0.025, 0.25 and 2.5 mM were added into medium for 3 days culturing. After colchine treatment, explants were subculture into a fresh medium for eight-weeks acclimation. Among three age explants, it was found that 10-week-rhizome had the hightst tetraploid induction rates from 10-50% in various concentration of colchicine. Moreover, The survival rates of rhizome explants were found decreasing as colchine concentration increased. Age of 13-week-rhizome explants were cultured in a medium containing with 0, 0.025, 0.25 and 2.5 mM colchicines for one week before subculturing into a solid fresh culture medium for eight weeks acclimation. The results showed that as concentration increasing to 0.25 and 2.5 mM, survival rates were decrease to 32.7 and 27.2%, respetively. However, a higher percentage of tetraploids, 30 and 20% was obtained from the 0.25 and 2.5 mM colchicine treatment. Colchicine of 0, 0.025, 0.25 and 2.5 mM were added into a solid or liquid culture medium for polyploidy induction using 12-week-rhizome explants. Treatment duration was last two weeks before subculturing into a fresh solid culture medium without colchicines for eight weeks acclimation. The results showed higher survival rates (21-35%) and percentages of tetraploids (5-30%) in liquid medium than that of in solid medium. Various treatment duration of 16, 24, 48 and 72 hr was compared using 10-week-rhizome culturing in a liquid medium containing 2.5 mM colchicine. The survival rates of explants decreased as the treatment duration increased. Treatment duration as short as 16 hr was found capable to induce tetraploid plants with 10% induction rate. Measurements of stoma size and its distribution density in leaves of polyploidy plants induced from anti-microtubule chemical treatment were conducted. A positive correlation between stoma size and ploidy level was obtained. Furthermore, the decreasing in stoma distribution density along with higher polyploidy level was also found. In conclusion the polyploidy induction experiments of in vitro A. formosanus Hayata in this study, using nodal explants culturing in a medium containing 2.5 mM colchicine for 3 days had the best result. The polyploidy level of regenerated plantlets was determined use young leaves by flow cytometry. Leaf stoma parameters observed by microscopy could also use for assistance polyploidy level detection.
30
chün, wei shih, e 魏士竣. "Molecular assay System for Authentication Study for Anoectochilusformosanus Hayata". Thesis, 2007. http://ndltd.ncl.edu.tw/handle/87412884846387378501.
Testo completoAbstract (sommario):
碩士亞洲大學
生物科技學系碩士班
95
Taiwan jewel orchid (Anoectochilus formosanus Hayata) is an indigenous Orchidaceae found in Taiwan. Extract of the whole plant had been applied for liver protection, anti-inflammation, pain relief, blood sugar reduction and blood pressure reduction. Taiwan jewel orchid had been confused and possibly be replaced by Ludisia discolor, A. koshunensis or Goodyera matsumurana, and Indonesian species in Taiwanese herbal markets. Because the whole plant morphology and outlooks of the mentioned herbs are not easily distinguished. In this study, molecular identification system for authentication of Taiwan jewel orchid had been established using 5.8S rDNA-ITS sequential difference analysis with PCR-RFLP based analysis, multiplex PCR techniques and bio-chip, ISSR (inter simple sequence repeats) and RAPD (Random amplified polymorphic DNA) molecular targeting. The results from 5.8S rDNA-ITS sequential difference analysis showed that the similarity between Taiwan jewel orchid and the “flaws” were between 90-99 %. However, Taiwan jewel orchid and the flaw species could also be distinguished with PCR-RFLP analysis by ApaL I、BssS I、BstB I and Ssp I restriction enzymes. By using specific designed primers and probes and comparing the differences of 5.8S rDNA-ITS between Taiwan jewel orchid and the flaws species with multiplex PCR and bio-chip. The result of ISSR primers and RAPD, 2 of them showed polymorphous bands patterns that were useful for distinguishing Taiwan jewel orchid from other “flaw” species after PCR amplification. This result indicated that the methods are more rapid, accurate and applicable in identification of Taiwan jewel orchid at the molecular level.
31
Huang, Jon-Kway, e 黃榮貴. "Anti-Fatigue Activity of Extracts of Polygonatum altelobatum Hayata". Thesis, 2012. http://ndltd.ncl.edu.tw/handle/72222084197384774185.
Testo completoAbstract (sommario):
碩士大仁科技大學
製藥科技研究所
100
Polygonatum altelobatum Hayata plants of the genus Polygonatum Liliaceae, is a Taiwan''s endemic plant, strengthen lung function, lowering blood sugar, strong bones and muscles, antifatigue and delay many efficacy of human aging. The present study is to investigate the effect of aqueous extracts from root of Polygonatum altelobatum Hayata (TPAE) on amelioration of exercise-induced fatigue and oxidative stress in rats. Sprague-Dawley (SD) rats were divided into four groups including control (fed with drinking water) and three groups of different doses of TPAE (75, 150 and 375 mg/kg.bw). An exhaustive exercise test on a treadmill and the measurement of biochemical parameters related to fatigue were carried out after eight weeks. The results revealed that TPAE could extend significantly the endurance time to exhaustion, as well as decreasing the blood lactate and blood urea nitrogen (BUN) contents. Malondialdehyde (MDA) level of rats in PAHE treated groups were significantly decreased compared with that in the control group, while superoxide dismutase (SOD) and catalase (CAT) were significantly increased with comparison to that in the control group. The overall results indicated that PAHE had anti-fatigue activity with effective prevention of oxidative stress after exhaustive exercise and could elevate the exercise tolerance.
32
Su, HSIANG-LING, e 蘇香伶. "Evaluation of the Antioxidative Characteristics from Cinnamomum insularimontanum Hayata". Thesis, 2018. http://ndltd.ncl.edu.tw/handle/7t9pdc.
Testo completoAbstract (sommario):
碩士輔英科技大學
保健營養系碩士班
106
Cinnamomum insularimontanum Hayata is a medium-sized evergreen tree, which grows in Taiwan, Indonesia and neighboring regions. Since ancient times, cinnamons were known for their antipyretic and anti-inflammatory effects. With aromatic properties, cinnamons were widely used as spices, and also considered as economically important plants. Recent studies indicated that Lauraceae plants not only have antibacterial and antioxidative properties, but inhibition of cancer cell proliferation and diabetes glucose control, etc. However, rare studies on the chemical com-positions and antioxidative properties of C. insularimontanum Hayata have been explored. In this study, the antioxidant activities of the ex-tracts by different solvents from the leaves, branches, barks and stems of C. insularimontanum were studied. Various analytic assays, including DPPH radical scavenging capacity, reducing power, ferrous ions chelating assay, total phenols and flavonoids contents were determined. Results indicated that ethanolic extracts from branch and bark of C. insularimontanum had better DPPH radical scavenging capacity 89.11% and 87.94% clearance rate than others (p <0.05). In addition, aqueous ex-tracts from branch and bark of C. insularimontanum had better reducing power 1266.47 μg/mL AAE, ferrous ions chelating capability 95.32%, and 93.02% chelation rate than others (p <0.05). Extracts from bark of C. insularimontanum had highest total phenol contents 652.63 and 655.16μg/ mL GAE than others. On the other hand, extracts from leaves of C. insu-larimontanum had highest flavonoid contents 330.58 and 223.61μg/mL QE than others (p<0.05). In conclusion, antioxidant effects of extracts from branch and bark of C. insularimontanum were much better than others (p <0.05). The results of volatile analysis and antioxidant analysis showed that the GC/SM analysis of C. insularimontanum essential oil had the highest ratio of Eucalyptol among monoterpene compounds with the DPPH free radical scavenging ability of 79% clearance rate, and reducing power of 1266.47 μg/mL AAE (p<0.05). Essential oil had higher total phenol contents of 338.36 μg/mL GAE and higher flavonoid contents of 155.27 μg/mL QE (p<0.05). C. insularimontanum extracts could be de-veloped as more potentially economical applications in agricultural, cos-metic and food products in the future.
33
Chiang, Nien-Ting, e 江念庭. "Cloning and Characterization of Pinoresinol-LariciresinolReductase from Taiwania cryptomerioides Hayata". Thesis, 2016. http://ndltd.ncl.edu.tw/handle/15376934980480272082.
Testo completoAbstract (sommario):
碩士國立臺灣大學
森林環境暨資源學研究所
105
Lignans, which are synthesized by dimerization of two C6-C3 units, exist abundantly in the wood of Taiwania cryptomerioides Hayata. Lignans in Taiwania, such as dimethylmatairesinol, taiwanin A and taiwanin E, were reported to have siginificant cytotoxic activity against tumor cells. As the key enzyme of lignan biosynthesis, pinoresinol-lariciresinol reductase (PLR) catalyzes the two-step reduction of pinoresinol to form lariciresinol and then to secoisolariciresinol. Secoisolariciresinol would next be oxidized by secoisolariciresinol dehydrogenase (SDH), and the product matairesinol is suggested to be the precursor for other downstream ignans. In this study, three PLR and six SDH candidate genes of T. cryptomerioides were identified by analysing the transcriptome of wood cambium, sapwood, transition zone and heartwood. The complete coding sequences (CDS) of each gene were obtained by rapid amplification of cDNA ends (RACE). Using Escherichia coli as the host, the heterologously expressed TcPLRs exhibited PLR activity, but the TcSDHs did not exhibited SDH activity. Among the three TcPLRs, TcPLR1 reduced pinoresinol to lariciresinol, while TcPLR2.2 and TcPLR3 reduced both pinoresinol and lariciresinol. The results of RT-PCR revealed that TcPLR1 most highly expressed in the wood cambium, and next in the heartwood. TcPLR2.2 and TcPLR3 both expressed in the wood cambium and the sapwood, while TcPLR2.2 showed slightly higher expression level in the cambium, and TcPLR3 mostly expressed in the sapwood. This study characterizes three PLRs of T. cryptomerioides, which involve in the biosynthesis of upstream lignans, and the expression pattern of TcPLR genes may provide information for identifying downstream lignan biosynthetic genes in the future.
34
CAI, CHUN-YING, e 蔡淳瑩. "An investigation of yield components in tankan (citrus tankan hayata)". Thesis, 1993. http://ndltd.ncl.edu.tw/handle/32711748769337786468.
Testo completo
35
HUANG, JING-HUI, e 黃菁蕙. "Antihypertensive mechanisms of phellodendron wilsonii hayata et kanehira in rats". Thesis, 1993. http://ndltd.ncl.edu.tw/handle/61561189347577214330.
Testo completo
36
Li, Chun-Yi, e 李俊毅. "Optimal Research of Artificial-Lighting System for Anoectochilus Formosanus Hayata". Thesis, 2010. http://ndltd.ncl.edu.tw/handle/82981559263405568443.
Testo completoAbstract (sommario):
碩士和春技術學院
電機工程研究所
99
Abstract The fluorescent lamp is the most frequently used artificial light source for plant cultivation. However, the spectrum of fluorescent lamps is different from solar spectrum significantly. The fluorescent lamp combined with blue LED and red LED are used to carry on Anoectochilus Formosanus Hayata experiments in this thesis. By means of the three light with different wavelength or illumination for experiments, we observe and record the live history of Anoectochilus Formosanus Hayata to find the best growing condition for high productivity. In the experiments, we set the fluorescent lamp under 1200 Lux, 1600 Lux and 2000 Lux. The blue LED is operated separately in 4W, 7W and 10W; and the red LED operates in the same conditions. We find Anoectochilus Formosanus Hayata grows best under the fluorescent lamp with 1600 Lux, blue LED with 10W and red LED with 7W. The optimum optical source combination decreases the growing time from six months to three months under resulting in the same compositions.
37
Lin, Chien-Hung, e 林建宏. "The constituents and their related activities of Hygrophila pogonocalyx Hayata". Thesis, 2009. http://ndltd.ncl.edu.tw/handle/26722249445468393158.
Testo completoAbstract (sommario):
碩士臺北醫學大學
生藥學研究所
97
Hygrophila pogonocalyx Hayata is an endemic species in Taiwan. It was found along the seashore in Taichung country and was endangered according to the Red List Categories of the International Union for Conservation of Nature and Natural Resources (IUCN). Our previous results showed that the 95％ ethanol extract of Hygrophila pogonocalyx exhibited free radical scavenging activities (HO•, O2•‾ and ABTS•‾, IC50 = 0.78, 12.75 and 7.94 ?慊/mL). Therefore, the active constituents of H.pogonocalyx from tissue cultures (providing by Dr. Chin-Wen Ho, Tatung University, Taipei) are isolated in the present study. The structures of isolated compounds were identified by various physical and spectroscopic characterizations (eg. ESI-MS, UV, IR and NMR etc.). Thirteen compounds from Hygrophila pogonocalyx Hayata were identified in this study, including two flavones, luteolin 7-O-β-D-glucopyranoside (1) and luteolin 7- O-β-D-glucuronide (2); four flavonols, quercetin (3), isoquercitrin (4), rutin (5) and myricetrin (6); three phenylethanoid glycosides, acteoside (7), isoacteoside (8) and β-ethoxyl-acteoside (9); two alkylated glycosides, 3-O-[β-D- apiofuranosyl-(1→6)-β-D-glucopyranosyl]oct-1-en-3-ol (10) and 3-O- [β-L-xylo- pyranosyl-(1→6)-β-D-glucopyranosyl]oct-1-en-3-ol (11) and two steroid, β-sitosterol (12) and stigmasterol (13). Those 9 compounds were further evaluated the neurocytoprotective activity in NGF-differentiated PC12 cells, and compound 1 shows the most potent activity. Those 11 compounds were also evaluated the inhibition of cellular tyrosinase activities in human epidermal melanocytes（HEMn）. Cell viabilities of compounds 1 ~ 11 were higher than 85％, the compound 7 was the most potent in inhibiting tyrosinase activity.
38
Huang, Yu-Ting, e 黃鈺婷. "Genetic diversity of Pinus morrisonicola Hayata based on DNA markers". Thesis, 2003. http://ndltd.ncl.edu.tw/handle/96803435185733034132.
Testo completo
39
Tseng, Sue-Chen, e 曾素貞. "Antibacterial effect and mutagenic assessment of Taiwan Pinus morrisonicola hayata". Thesis, 2006. http://ndltd.ncl.edu.tw/handle/14592410301809787750.
Testo completoAbstract (sommario):
碩士國立屏東科技大學
食品科學系
94
The current study aims at extracting three different parts of Taiwan Pinus morrisonicola hayata with three kinds of solvent. The extract of needle, bark and cone was collected for analyzing product profile, antibacterial and prebiotic effect, and assessing toxicity and mutagenesis. The sample was extracted from Taiwan Pinus morrisonicola hayata by methanol, ethyl acetate, and distilled water, and the methanol showed the best yield of extraction. For cone and needle, the multiple substances were extracted by methanol, while the highest quantity was obtained in bark when water was employed as an extraction solvent. The antibacterial and prebiotic result showed that the methanol cone extraction of Taiwan Pinus morrisonicola hayata with the concentration 40 mg/ ml moderately suppresses the C. perfringens when incubating at 37℃ for 5 hours. For Salmonella enterica, Staphylococcus aureaus, the water cone extraction with the concentration 20 mg/ ml indicated that no significant inhibitory effect were observed comparing with the control, and the repressing ability limited increase even extend the incubation time. In related to prebiotic study, the water bark extraction with the concentration 40 mg/ml suggested that little increase on the growth of Lactobacillus acidophilus was observed. Furthermore, the water cone extract with the concentration 20 mg/ml illustrated that no obvious difference has been found if Bifidobacterium bifidum was employed as a subject. In the Ames test, regarding Salmonella typhimurium TA98 and TA100, limited toxicity and mutagenesis were found in the range of 0.5-5.0 mg/plate. Conclusively, the selected extract of Taiwan Pinus morrisonicola hayata, at least, has antibacterial effect on C. perfringens, and no toxicity and mutation were detected under specified conditions. However, the result of the present work can provide useful information for health food development.
40
Tsai, Yi-Chi, e 蔡依錡. "Genetic Variation of Juniperus formosana Hayata Based on ISSR Markers". Thesis, 2004. http://ndltd.ncl.edu.tw/handle/47189512558068349296.
Testo completoAbstract (sommario):
碩士國立中興大學
生命科學系
92
Juniperus formosana is generally distributed in mountain grasslands and rocky habitats above 2,500m in altitude in Taiwan. Few populations also occur in lower altitude on the east side of the island. It is a representative first or second successive tree species of the high mountain plant community. In the present study, 117 individuals from 8 populations were studied for ISSR variation. One hundred and twenty-six bands were obtained using 10 primers. Eighty bands (63.49%) were polymorphic bands. AMOVA analysis revealed that the perceontages of variance components attributable to variation among regions, among population within regions, and among individuals within populations were 5.26% (P<0.001), 18.96% (P<0.001), and 75.78% (P<0.001), respectively. POPGENE analysis revealed that the total gene diversity (Ht) of the species was 0.3536 and the gene diversity of populations ranged from 0.1593 to 0.2458. The population genetic diversity index (Gst) was 0.2177 while the gene flow index (Nm) was 1.797. The result indicated that the population differentiation was higher than the average of conifer species. There is a significant genetic difference between Liu-Shue population of Hualien County and the rest population. Sian-Yang population is significantly different from Yushan and Guanshan population of the same southern region. Shieshan population is significantly different from Dabagienshan population of the same moutain range.
41
Hung, Chien-Cheng, e 洪建成. "Designing a Dimmable Artificial Lighting Source for Anoectochilus Formosanus Hayata". Thesis, 2012. http://ndltd.ncl.edu.tw/handle/99284139309807720277.
Testo completoAbstract (sommario):
碩士和春技術學院
電機工程研究所
100
Some particular wavelengthsof light, such as the red part and the blue, are the most essential part for photosynthesis.There is a kind of substance, riboflavin, can turn solar energy into nutrition which is needed by plants.The thesiswill focus on developing high efficiency and dimmable controlled backlight driving systems for cold cathode fluorescent lamps (CCFLs) and light emitting diodes (LEDs). By the way, this combined light source could increase the growth in each stage of plants: seedlingsin flasks, juvenile, and large seedlings. This thesisproposes a novel indoor plant-lighting scheme in which red LEDs, blue LEDs and CCFLs are combined to work as effective light sources. The combinations examined in this thesishave been proven effective in improving the growth uniformity of Anoectochilus Formosanus Hayata.Finally, the driver is implemented to drive a light source combined by CCFL, R-LED andB-LED. Experimental results show that the proposed circuit can be obtained the highest efficiency is 86.3%.
42
Liu, Shu-Ju, e 劉淑如. "Establishment of multiplication protocols on themedicinal plant Glycine tomentella Hayata". Thesis, 2019. http://ndltd.ncl.edu.tw/cgi-bin/gs32/gsweb.cgi/login?o=dnclcdr&s=id=%22107NCHU5105045%22.&searchmode=basic.
Testo completoAbstract (sommario):
碩士國立中興大學
生命科學院碩士在職專班
107
In this study, the proliferation system of the medicinal plant of Glycine tomentella Hayata (one root of kinmen) was established by the micropropagation methods, including seed germination methods, establishments of sterile seedlings, inductions of shoots and adventitious roots from explants, comparisons of B5 and MS media for shoot multiplications, and acclimation and transplanting of seedlings in soils. The seed germination test results showed that the germination rate of peat soil sown in the B5 solution with half-amount of macroelements and 1.5% sucrose was 52.2%, whereas the germination rate in the same media without sucrose was 45.6%, suggesting additions of sucrose may enhance seed germination rates of Glycine tomentella Hayata. Both MS and B5 media were tested for shoot multiplications. Results showed that when explants grown on MS media in comparison to explants grown on B5 media for 12 weeks, plant heights were shorter, numbers of cluster buds from explants were fewer, leaves were light green, thin, and large. Additionally, when explants grown on B5 media with 0.4 ppm BA and 0.04 ppm NAA compared to explants grown on MS media with same concentration of BA/NAA for 12 weeks, plant grew more vigorously, cluster bud numbers from explants were more, leaves were greener and smaller, indicating B5 media with BA and NAA were more suitable for shoot multiplications. Additions of plant growth regulators and locations of buds were examined to determine their effects on shoot multiplications of the Glycine tomentella Hayata explants. Results showed that when explants grown on B5 media with 0.1/0.01 ppm BA/NAA and 0.2/0.02 ppm BA/NAA had higher numbers of shoots than other tested media. The average number of shoots per explant was 8.3 when explants grown on B5 media with BA and NAA for 12 weeks. The rooting rate of the Glycine tomentella Hayata explants was 97.8% when grown on B5 media with 0.2-0.8 ppm IBA for 12 weeks. When grown on B5 media with 0.4 and 0.8 ppm IBA for 12 weeks, root numbers per explant were 13.8 and 12.4 which were higher than other tested media. Rooting rate of explants was 95.6% when grown on B5 media with 0.4 and 0.6 ppm NAA after 12 weeks. Root numbers per explant were 19.4 and 18.6 on these two media that were higher than other tested media. The bud was inverted to examine its effect on rooting of the Glycine tomentella Hayata explants. Results showed no roots were obtained when explants grown on B5 media iii with or without plant growth regulators. The Glycine tomentella Hayata seedlings were transplanted in soil for 30 days, survival rates of seedlings were 100%, suggesting optimal tissue culture protocols for the Glycine tomentella Hayata sterile seedling proliferations were successful established in this study.
43
陳冠丞. "The Antioxidative and Bioactive Componentsof Root Extract from Glycine tomentella Hayata". Thesis, 2002. http://ndltd.ncl.edu.tw/handle/56669096501901119633.
Testo completoAbstract (sommario):
碩士國立海洋大學
食品科學系
90
I-Tiao-Gung, root of Glycine tomentella Hayata, used in this study was about 12.0 ~ 14.3 cm in length. The polyphenol content expressed as gallic acid equivalent extracted from I-Tiao-Gung using water, 50 % ethanol and 95 % ethanol as solvent were 34.82, 103.72 and 88.54 mg/g respectively. Extraction with 95 % ethanol gave the highest yield (12.0 %). Inhibition of hemoglobin-induced linoleic acid (C18:2) oxidation by root extracts of I-Tiao-Gung was indicated by the IC50 being 4.63, 9.06, 28.16 μg/ml, for the 95 % ethanol, 50 % ethanol, water extract, decreased with increased polarity of the extraction solvent. All three crude extracts had free radical-scavenging activity determined with DPPH. The capacity was similar with inhibition of lipid autoxidation in an order of 95 % ethanol extract > 50 % ethanol > water. Inhibition on the soybean lipoxygenase catalyzed arachidonate oxidation was the highest with 95 % ethanol extract, and followed the same order in reducing 15-hydroperoxy eicosatetraenoic acid (15-HpETE) formation shown by HPLC, and chemiluminescence intensity using luminol. All three crude extracts chelated iron ion, while the lipoxygenase active site has Fe3+. Blood thinning effects were observed in vitro by mixing the extract of I-Tiao-Gung with human red blood cells. The rheological data fitted the power law model, σ = K γ n, where σ =shear stress, γ = shear rate, K = fluid consistency, n =flow behavior index, with a correlation coefficient of R2 = 0.99 at a constant hematocrit of 44 %. The addition of extract resulted in a reduced K from 0.13 to 0.08, and an increased n from 0.65 to 0.71 indicative of a change in flow behavior closer to Newtonian type and a thinning consistency. Separation of the crude 95 % ethanol extract of I-Tiao-Gung using polyamide column eluted by water, 10 % ethanol 30 % ethanol, 50 % ethanol, 70 % ethanol, 95 % ethanol, methanol-chloroform (1:1), and methanol as mobil phase, obtained one fraction eluted by 50 % ethanol showing a purity of 88.6 % as detected with A254nm. The fraction had color reactions and UV-Vis spectrum similar to those of epicatechin. It inhibited linolate autoxidation, and lipoxygenase activity, as well as DPPH free radical scavenging, but did not chelate iron.
44
Lin, Wen-Juain, e 林玟娟. "The population genetic structure of Impatiens uniflora Hayata (Balsaminaceae) in Taiwan". Thesis, 1995. http://ndltd.ncl.edu.tw/handle/51911523456113800553.
Testo completo
45
Lin, Wen Juan, e 林玟娟. "The population genetic structure of impatiens uniflora hayata (balsaminaceae) in Taiwan". Thesis, 1995. http://ndltd.ncl.edu.tw/handle/40420946447658750761.
Testo completo
46
Chen, Xien Tein, e 陳心恬. "Chemical Investigations of the Methanolic Extract of Cinnamomum kanehirae Hayata Leaves". Thesis, 2015. http://ndltd.ncl.edu.tw/handle/v27xww.
Testo completo
47
Lee, Wu-Lin, e 李武林. "Genetic Diversity in Mahonia oiwakensis Hayata (Berberidaceae) Investigated by ISSR Markers". Thesis, 2007. http://ndltd.ncl.edu.tw/handle/98603205088228772262.
Testo completoAbstract (sommario):
碩士國立嘉義大學
森林暨自然資源研究所
95
Mahonia oiwakensis Hayata is an endemic species in Taiwan belonging to Berberidaceae. The genetic diversity and geographical relationship of 122 individuals sampled from 11 nature populations in Taiwan were examined using ISSR fingerprinting. There were 12 ISSR primers used in PCR and amplified 119 bands, 61 of them were polymorphism bands（51.26％）. AMOVA revealed that the component of variance among populations was 28.81%（p＜0.001）and attributed to the population differentiation. The component of variance within population was 71.19%, that shown high and significant（p＜0.001）. Based on Mantel test, correlation coefficients among genetic variation and the geographical distribution and elevation of 11 nature populations were not significant. Analysis of Popgene demonstrated that there are remarkable genetic diversity（genetic diversity = 0.3710, Gst = 0.3942）and low level of gene flow（Nm = 0.7684）. The results of this analysis were probably due to the influence from the different environment that has restricted the gene flow and promoted a higher genetic differentiation. Cluster analysis and Principal Coordinate analysis（PCOA）similarly revealed that the genetic differentiation may be caused by different environments while habitat influences and space order are not be corresponding to the geographical relationships.
48
Ruan, Chang-Ting, e 阮昌庭. "Molecular characteristics of the water-soluble polysaccharides from Anoectochilus formosanus Hayata". Thesis, 2005. http://ndltd.ncl.edu.tw/handle/61724964623257140978.
Testo completoAbstract (sommario):
碩士國立臺灣大學
食品科技研究所
93
Cytokine stimulating activity of Anoectochilus formosanus on human peripheral blood nuclear cells has been associated with its water-soluble polysaccharides. To study the molecular characteristics of these polysaccharides, the polysaccharide enriched fractions were prepared from hot-water extracts of whole plant through protease and amylase digestion and collected with ethanol precipitation. The yield of water-soluble polysaccharide fraction (AFP) accounted for 3.03% of dried mass. The AFP was further separated into neutral (AFPN) and acidic (AFPA) fraction by DEAE-anion-exchange chromatography with yields of 47.6% and 30.9% of AFP, respectively. The AFPN was composed of two linear homopolysaccharides, a galactan and a mannan, which can be separated by selecting precipitation of Fehling’s solution through copper (II) ion and mannan complex formation. The glycosidic linkage of both polysaccharide were found to be β-(1→4) revealed by 13C-NMR analyses. The apparent molecular weight of the galactan and the mannan were 21.2 and 52.7 kDa, respectively, according size-exclusion chromatographic analyses. The acidic polysaccharide fraction (AFPA) was found to be a complicate polysaccharide mixture. The major components of acidic fraction were pectic polysaccharides which were compose of 78.6% of galacturonic acid and showed characteristic of rhamnogalactan II and (1→3, 1→6)-β-D-galactan. One proteoglycan was collected in acidic fraction from low anionic strength eluting portion of DEAE-chromatography. The proteoglycan contents 58.4% protein and 41.6% glycan with molecular weight of 51.5 kDa. The sugar compositions of the glycan were arabinose, galactose, glucose and mannose in a molar ratio of 29.8: 35.1: 10.3: 23.8.
49
Tzeng, Shih-Han, e 曾詩涵. "Tissue culture of Anoectochilus formaosanus Hayata and quantitative analysis of gastrodin". Thesis, 2005. http://ndltd.ncl.edu.tw/handle/30828717705707840904.
Testo completoAbstract (sommario):
碩士國立中興大學
生命科學系
93
Anoectochilus formosanus Hayata (Orchidaceae) belongs to a group of terrestrial orchids commonly known as “Taiwan Jewel Orchids”, because of their attractive foliage. A. formosanus, one of the nourishing and health drugs, is a popular folk medicine that possesses antioxidant, anti-inflammatory and liver-protective effects and has been used to cure hepatitis, hypertension and cancer in Taiwan. The goal of this study was to test the influence of various strength of MS basal salts and organic additives such as banana pulp and mashed potato on the in vitro shoot growth of A. formosanus. The experimental results indicated that medium containing 1/2 MS basal salts and a mixture of mashed potato (30 g/l) and banana pulp (50 g/l) was found to be the best combination for the growth of lateral buds and plantlets of A. formosanus. The growth of the shoots reduced at each subculture and the generations C3 and C4 resulted in 0.8-33% albino plants. Variation in the survival rate was also observed in these tissue-cultured plants transferred to peat moss and cultured in greenhouse for eight months. Greenhouse-grown plants at earlier growth stage contain higher amounts of micronutrients. After 8 months of transplantation, the contents were found to be close to those of wild type. Heavy metal concentrations in plants grown in greenhouse were also determined and showed lower than those of wild type. Gastrodin, an active phenolic ingredient of A. formosanus have been quantitatively estimated using High Performance Liquid Chromatography (HPLC). Maximum production was observed in one-month-old greenhouse-grown plants. After eight months, the amount of gastrodin in greenhouse grown plants was found to be similar to that of wild type.
50
Hsieh, Yaw-Lung, e 謝耀隆. "Study on the Constituents of the Heartwood of Pseudotsuga wilsoniana Hayata". Thesis, 1996. http://ndltd.ncl.edu.tw/handle/44374484749443878453.
Testo completo