Bibliografie tematiche / Fluorescence

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Letteratura scientifica selezionata sul tema "Fluorescence"

Autore: Grafiati
Pubblicato: 4 giugno 2021
Ultima modifica: 12 ottobre 2024

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Articoli di riviste sul tema "Fluorescence"

1

MATSUMOTO, TAKURO, ATSUSHI SUETSUGU, KOSUKE HASEGAWA, MIKI NAKAMURA, YUHEI SHIBATA, HITOMI AOKI, TAKAHIRO KUNISADA et al. "A Mouse Model of Fluorescent Protein-expressing Disseminated Peritoneal Lymphoma for Fluorescence-guided Surgery". Anticancer Research 36, n. 9 (9 settembre 2016): 4483–88. http://dx.doi.org/10.21873/anticanres.10993.

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2

Pawley, James B. "Fluorescence Microscopy and Fluorescence Probes". Microscopy and Microanalysis 4, n. 2 (aprile 1998): 164–65. http://dx.doi.org/10.1017/s1431927698000166.

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Fluorescence Microscopy and Fluorescence Probes, edited by Jan Slavik, 1996. Plenum Press, New York and London. 306 pages. (hardback, $95)This volume is a compilation of abstracts of papers presented at the Fluorescence Microscopy and Fluorescence Probes Conference held in Prague in June 1995. This conference was one of the first tangible results of the end of the Cold War in the field of microscopy. As such, it attracted a great many of those who constitute the vanguard of progress in the field of fluorescence microscopy, on both sides of the old East-West divide. About one-third of the 127 contributors were from the West.
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Sessions, S. K. "Fluorescence Microscopy; Quantitative Fluorescence Microscopy". Systematic Biology 42, n. 2 (1 giugno 1993): 224–25. http://dx.doi.org/10.1093/sysbio/42.2.224.

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4

Ravinson, Daniel Sylvinson Muthiah, e Mark E. Thompson. "Thermally assisted delayed fluorescence (TADF): fluorescence delayed is fluorescence denied". Materials Horizons 7, n. 5 (2020): 1210–17. http://dx.doi.org/10.1039/d0mh00276c.

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Thermally assisted delayed fluorescence (TADF) allows for efficient collection of both singlet and triplet excitons with both emitting through the singlet channel. TADF opens the door to photo- and electroluminescence efficiencies close to 100%.
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Heldt, Józef, Janina R. Heldt e Jerzy Kamiński. "Steady-state and Time-resolved Spectroscopic Studies of Benzanilides". Zeitschrift für Naturforschung A 54, n. 8-9 (1 settembre 1999): 495–502. http://dx.doi.org/10.1515/zna-1999-8-909.

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Steady-state and time-resolved spectroscopic studies of benzanilide (I) and jV-methylbenzanilide (II)were performed at 298 and 77 K in various solvents. The results indicate that benzanilide fluorescencein non-polar solvents at room temperature involves three independent modes of emission: F1 (LE) normalfluorescence from the initially excited state S1 (LE) with λmax = 320 nm, F2´(PT) fluorescence from the proton transfer tautomer with λmax = 468 nm, F2″CT) fluorescence from the species where intramolecular charge transfer appears, with λmax = 510 nm. At 77 K in MCH a new fluorescence band, Fag, appears at λmax=415 nm instead of the F2(PT) and F2″CT) fluorescence. This new emission originates from benzanilide dipolar aggregates or cis-imidol dimers. The decay times of these emission modes aredifferent.N-methylbenzanilide, dissolved in nonpopular and weakly polar solvents at room temperature and at77 K, shows only two fluorescence modes, i.e., the normal and the charge-transfer emissions at 320 nmand 520 nm, respectively. The fluorescence is deactivated with two decay times, 30 ps and 2.05 ns, inMCH solution.
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Ping Tang, Ping Tang, e Luping Lyu and Yujin Li Luping Lyu and Yujin Li. "Fluorescence and Theoretical Calculation of Phenylhydrazone Derivatives and Fluorine Boron Complex: Synthesis and Fluorescence Characteristics". Journal of the chemical society of pakistan 42, n. 1 (2020): 10. http://dx.doi.org/10.52568/000623.

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A sereis of phenylhydrazone-based derivatives and their corresponding BF2 complexes were synthesized efficiently by a three-step reaction. Photophysical performance was investigated in different organic solvents and in the solid state. Although these compounds exhibited feeble fluorescent intensity in solution-state, BF2 complexes showed weaker fluorescence in solid state compared to precursors 2, which were caused by slight geometry relaxation of upon photo excitation. Density Functional Theory calculations was carried out to confirm above inference.
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Ping Tang, Ping Tang, e Luping Lyu and Yujin Li Luping Lyu and Yujin Li. "Fluorescence and Theoretical Calculation of Phenylhydrazone Derivatives and Fluorine Boron Complex: Synthesis and Fluorescence Characteristics". Journal of the chemical society of pakistan 42, n. 1 (2020): 10. http://dx.doi.org/10.52568/000623/jcsp/42.01.2020.

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A sereis of phenylhydrazone-based derivatives and their corresponding BF2 complexes were synthesized efficiently by a three-step reaction. Photophysical performance was investigated in different organic solvents and in the solid state. Although these compounds exhibited feeble fluorescent intensity in solution-state, BF2 complexes showed weaker fluorescence in solid state compared to precursors 2, which were caused by slight geometry relaxation of upon photo excitation. Density Functional Theory calculations was carried out to confirm above inference.
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BEARDER, E. ARTHUR. "Fluorescence". Journal of the Society of Dyers and Colourists 27, n. 12 (22 ottobre 2008): 270–79. http://dx.doi.org/10.1111/j.1478-4408.1911.tb00530.x.

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9

Inoue, S., O. Shimomura, M. Goda, M. Shribak e P. T. Tran. "Fluorescence polarization of green fluorescence protein". Proceedings of the National Academy of Sciences 99, n. 7 (2 aprile 2002): 4272–77. http://dx.doi.org/10.1073/pnas.062065199.

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Gardner, Craig M., Steven L. Jacques e Ashley J. Welch. "Fluorescence spectroscopy of tissue: recovery of intrinsic fluorescence from measured fluorescence". Applied Optics 35, n. 10 (1 aprile 1996): 1780. http://dx.doi.org/10.1364/ao.35.001780.

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Tesi sul tema "Fluorescence"

1

Nagl, Stefan. "Fluorescent multiple chemical sensing using time-domain fluorescence lifetime imaging". kostenfrei, 2008. http://www.opus-bayern.de/uni-regensburg/volltexte/2008/996/.

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Remy, Charlotte. "Synthèse et étude de récepteurs moléculaires fluorescents pour la détection de molécules neutres". Thesis, Université Paris-Saclay (ComUE), 2016. http://www.theses.fr/2016SACLN070/document.

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La détection de molécules toxiques pour l’Homme et son environnement est d’une importance cruciale et fait partie des préoccupations majeures de la société actuelle. Les résidus de pesticides tels que l’atrazine ainsi que la mélamine font notamment partie de ces molécules dangereuses pour la santé humaine. Ces deux molécules sont principalement dosées par des techniques lourdes et coûteuses comme la spectrométrie de masse, la chromatographie ou l’électrochimie. De même, la détection des amines biogéniques représente un intérêt sociétal. Elles sont produites par des bactéries durant la décarboxylation des acides aminés dans les cellules. Leur détection permet ainsi d’évaluer la contamination microbiologique et la dégradation potentielle d’un aliment. Elles sont aujourd’hui dosées par chromatographie en phase liquide ou en en phase gaz, par électrochromatographie capillaire et par spectroscopie UV-visible. Quelques exemples de détection par fluorescence ont déjà été décrits dans la littérature mais la nécessité de développer de nouveaux récepteurs fluorescents efficaces est bien réelle.La fluorescence est une technique qui offre de multiples avantages tels que la sensibilité, la sélectivité et un faible coût. De nombreuses sondes fluorescentes capables de détecter des métaux lourds ont été développées au laboratoire PPSM. Cependant, la détection de molécules neutres par fluorescence représente un défi supplémentaire en raison de la nature plus faible de l’interaction, comparée à celle entre espèces chargées.La première étape de cette thèse a été de concevoir et de synthétiser un ensemble de sondes moléculaires fluorescentes, aussi bien pour la détection de l’atrazine, de ses produits de dégradation et des dérivés de la mélamine que pour la détection des amines biogéniques. Des fluorophores dérivés de la molécule de maléimide, de naphthalimide et de l’acide barbiturique ont ainsi été développés pour sonder les dérivés de triazine en exploitant leur système de trois liaisons hydrogène pour la reconnaissance moléculaire. De même, un calix[6]arène fluorescent a été conçu pour déceler la présence des amines biogéniques qui provoqueront une réponse fluorescente par encapsulation dans le calixarène.La deuxième étape a consisté à étudier les propriétés photophysiques de ces sondes. La sonde Naphth-AlcyneOMe possède un rendement quantique élevé, s’est révélée fortement solvatochrome. Elle est de plus sensible à la déprotonation de sa fonction imide. Des études RMN et de modélisation moléculaire ont également été menées afin de caractériser les sondes de manière plus approfondie et de comprendre plus précisément leur réactivité. La spectroscopie RMN a confirmé l’interaction par liaison hydrogène entre les sondes maléimide et naphthalimides et la molécule d’atrazine. Elle a aussi mis en évidence l’encapsulation de l’heptylamine dans le calix[6]arène. Pour sa part, la modélisation moléculaire nous a permis de mieux comprendre la photophysique de la sonde Naphth-TriazoleOMe.Enfin, la capacité des sondes à détecter les divers analytes cibles par fluorescence a été évaluée lors de la dernière étape de ce projet. La sonde TPA-BARB a présenté une forte exaltation de fluorescence en présence des dérivés de mélamine alors que le calix[6]arène-quinoléine Calix-Quino est capable de détecter les amines aliphatiques par fluorescence
The detection of molecules toxic for man and his environment is one of the major concerns of our society. Melamine and the pesticide residues such as atrazine are some of these dangerous molecules. These two molecules are usually measured with time-consuming and costly techniques like mass-spectrometry, chromatography or electrochemistry. In the same way, the detection of biogenic amines is of the greatest importance. They are produced by some bacteria during the decarboxylation of amino acids in the cells. So their detection allows to assess the microbiologic contamination and the potential degradation of a food. Today they are measured by chromatography in the liquid or gas phase, capillary electrochromatography and UV-visible spectroscopy. Some examples of detection by fluorescence have been described in scientific literature, but it is really necessary to develop some new efficient fluorescent receptors.Fluorescence is a technique which offers many advantages such as sensitivity, selectivity and a low cost. A lot of fluorescent probes able to detect heavy metals have been developed in PPSM laboratory. However the detection of neutral molecules by fluorescence represents an additional challenge as the interaction is weaker than with charged species.The first step of this thesis was to design and synthesize a set of fluorescent molecular probes designed to detect atrazine, the products of its degradation and melamine derivatives as well as biogenic amines. Some fluorophores based on maleimide, naphtalimide and barbituric acid moieties have been developed for the detection of the triazines derivatives by exploiting their three hydrogen bonds for molecular recognition. In order to detect the presence of biogenic amines, a fluorescent calix[6]arene which lead to a fluorescent change upon encapsulation in the calixarene cavity has been designed.The second step consisted in studying the photophysical properties of these probes. Naphth-AlcyneOMe probe which has a high quantum yield turned out to be highly solvatochromic. Moreover it is sensitive to the deprotonation of its imide function. NMR studies and molecular modeling were conducted in order to deepen the characteristics of the probes and better understand their reactivity. NMR spectroscopy confirmed the interaction through hydrogen bonding between maleimide and naphtalimide probes and the atrazine molecule.It highlighted the encapsulation of heptylamine in the calix[6]arene. Molecular modeling enabled us to better understand the photophysics of Naphth-TriazoleOMe probe.Finally the capacity of probes to detect the various analytes by fluorescence was assessed in our last part. TPA-BARB probe presented a high exaltation of fluorescence in presence of melamine derivatives whereas the calix[6]arène-quinoleine Calix-Quino is able to detect aliphatic amines by fluorescence
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Dennis, Allison Marie. "Quantum dot-fluorescent protein pairs as fluorescence resonance energy transfer pairs". Diss., Georgia Institute of Technology, 2009. http://hdl.handle.net/1853/37079.

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Fluorescence resonance energy transfer (FRET)-based biosensors have been designed to fluorometrically detect everything from proteolytic activity to receptor-ligand interactions and structural changes in proteins. While a wide variety of fluorophores have demonstrated effectiveness in FRET probes, several potential sensor components are particularly notable. Semiconductor quantum dots (QDs) are attractive FRET donors because they are rather bright, exhibit high quantum yields, and their nanoparticulate structure enables the attachment of multiple acceptor molecules. Fluorescent proteins (FPs) are also of particular interest for fluorescent biosensors because design elements necessary for signal transduction, probe assembly, and device delivery and localization for intracellular applications can all be genetically incorporated into the FP polypeptide. The studies described in this thesis elucidate the important parameters for concerted QD-FP FRET probe design. Experimental results clarify issues of FRET pair selection, probe assembly, and donor-acceptor distance for the multivalent systems. Various analysis approaches are compared and guidelines asserted based on the results. To demonstrate the effectiveness of the QD-FP FRET probe platform, a ratiometric pH sensor is presented. The sensor, which uses the intrinsic pH-sensitivity of the FP mOrange to modulate the FP/QD emission ratio, exhibits a 20-fold change in its ratiometric measurement over a physiologically interesting pH range, making it a prime candidate for intracellular imaging applications.
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M'Baye, Gora Duportail Guy. "Sondes fluorescentes ratiométriques dérivées de la 3- Hydroxyflavone Etude spectroscopique de nouveaux dérivés et applications en biophysique membranaire /". Strasbourg : Université Louis Pasteur, 2007. http://eprints-scd-ulp.u-strasbg.fr:8080/755/01/MBAYE2007.pdf.

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Henderson, Julius Nathan. "Crystallographic and spectroscopic studies of photoswitching in fluorescent proteins /". view abstract or download file of text, 2007. http://proquest.umi.com/pqdweb?did=1417810431&sid=5&Fmt=2&clientId=11238&RQT=309&VName=PQD.

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Thesis (Ph. D.)--University of Oregon, 2007.
Typescript. Includes vita and abstract. Includes bibliographical references (leaves 143-151). Also available for download via the World Wide Web; free to University of Oregon users.
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Boujut, Margot. "Ligands Photo-Actifs pour l'imagerie de fluorescence du VEGFr". Thesis, Normandie, 2020. http://www.theses.fr/2020NORMR063.

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Les VEGFr (Vascular Endothelium Growth Factor receptors, récepteurs des facteurs de croissance de l’endothélium vasculaire) sont des protéines responsables de l’angiogenèse, c’està-dire, la croissance des vaisseaux sanguins. De fait, ils sont impliqués dans les maladies liées à une vascularisation néfaste comme dans la croissance des tumeurs cancéreuses ou de néovascularisation rétinienne. Traiter ces vaisseaux sanguins sans atteindre les tissus sains est un enjeu qui nécessite de les imager spécifiquement et précisément, ces deux critères pouvant être atteints par imagerie de fluorescence. La spécificité en imagerie de fluorescence repose sur l’emploi d’une sonde, c’est-à-dire un fluorophore sélectif du phénomène à imager. Pour synthétiser des sondes sélectives, nous nous sommes inspirés d’un ligand connu du VEGFr : l’axitinib. La structure chimique de l’axitinib comporte un hétérocycle indazole avec deux rôles essentiels : (i) dans la fluorescence de l’axitinib, (ii) dans sa sélectivité pour le VEGFr. Cette structure a été modifiée de façon à introduire des substituants rendant la molécule plus fluorescente tout en conservant au mieux le squelette responsable de l’activité biologique. Une librairie d’une vingtaine de fluorophores a été synthétisée et étudiée pour des applications en imagerie de fluorescence
VEGFr (Vascular Endothelium Growth Factor receptors) are proteins responsible for the angiogenesis, meaning the growth of blood vessels. Consequently, they are involved in diseases due to harmful vascularization such as tumor growth or retinal neovascularization. Treating those blood vessels without harming healthy tissues is an issue. It requires specific and precise images of the blood vessels, both criteria being achievable thanks to fluorescent imaging. The specificity of fluorescent imaging relies on the use of a probe, meaning a selective fluorophore. To synthetize selective probes, we were inspired by a known ligand of the VEGFr: the axitinib. The chemical structure of the axitinib has an indazole heterocycle with two key roles: (i) in the fluorescence of the axitinib, (ii) in its selectivity for the VEGFr. Substituents were introduced to increase the overall fluorescence of the molecule while preserving the backbone responsible for the biological activity to the best of our ability. A library of about twenty fluorophores was synthetized and studied for applications in fluorescent imaging
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Goulas, Yves. "Teledetection de la fluorescence des couverts vegetaux : temps de vie de la fluorescence chlorophyllienne et fluorescence bleue". Paris 11, 1992. http://www.theses.fr/1992PA112264.

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La mesure de la fluorescence de la chlorophylle in vivo est une methode d'investigation largement utilisee pour le suivi de l'activite photosynthetique, sur algues, chlorophastes ou feuilles entieres. Sur un couvert vegetal, la mesure a distance de la duree de vie de fluorescence permet de determiner directement le rendement quantique et peut ainsi etre envisagee comme methode de teledetection de l'etat physiologique de la vegetation. Dans cette perspective, nous avons etudie les possibilites qu'offre la fluorescence resolue en temps. Nous avons etudie l'effet du quenching energetique sur les composantes du declin de fluorescence et nous interpretons ces variations en termes de taille d'antenne et de limitation de la diffusion des excitons. Nous avons examine la faisabilite des mesures de duree de vie sur un couvert vegetal et defini les techniques instrumentales a mettre en uvre. Nous avons egalement developpe une methode specifique d'analyse qui permet de calculer la valeur de la duree de vie moyenne de fluorescence a partir des signaux emis par la canopee apres une impulsion lumineuse. A cote de l'emission rouge de la chlorophylle, les feuilles des vegetaux superieurs presentent une emission de fluorescence dans la region bleue du spectre apres excitation par un rayonnement ultraviolet. Cette emission a ete remarquee pour sa sensibilite au stress, mais son origine reste encore inconnue. Nous avons etabli les spectres d'excitation et d'emission des differents composes qui en sont responsables chez l'epinard, ce qui nous permet de proposer un certain nombre d'especes chimiques pour l'origine de cette emission. Nous avons etudie la faisabilite de sa detection a grande distance, simultanement avec l'emission chlorophyllienne, par la technique des lignes de fraunhofer et nous evaluons les performances attendues dans ce cas
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Cheng, Hok Yan. "Near infrared fluorescence probes : towards applications in fluorescence guided surgery". Thesis, University of Hull, 2017. http://hydra.hull.ac.uk/resources/hull:16529.

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Surgery has been a popular method for the treatment of cancers, in particular solid tumours; but the surgical margins for cancerous tissues are often indistinct and in most cases, the poor identification of residual cancer tissues can result in re-excision. Therefore, near infrared (NIR) fluorescence-guided surgery (FGS) is being developed as a real time intra-operative imaging technique to assist surgeons by improving the accuracy and precision of the removal of tumours. However, current FDA approved fluorophores suffer from poor chemical stability, limited water-solubility, and lack selectivity toward neoplastic tissue, limiting their clinical application. These current challenges have led to the development of new and improved fluorophores capable of absorbing and emitting light at NIR wavelengths, negating autofluorescence and improving deeper light transmission. Throughout this project, a series of BODIPYs, aza-BODIPYs and bacteriochlorins were synthesised and developed for bioimaging applications. Despite many of them showing interesting fluorescence properties, the investigation suggested aza-BODIPYs were the most promising red / NIR fluorophores (λem 600-700 nm) due to their excellent photostability. Methods have been developed to incorporate functionalities suitable for bioconjugation. Different bioconjugation strategies have been explored to covalently conjugate the NIR fluorophores to a clinically relevant protein, peptide and antibody under mild conditions. The viability of aza-BODIPY conjugates against biological targets were investigated and a range of other novel targeted NIR fluorophores were successfully developed. In vitro fluorescence imaging was subsequently carried out to demonstrate the enhanced selectivity of the targeting NIR fluorophores toward overexpressed receptors on various cancer cells lines. This project has demonstrated the potential of aza-BODIPY in biological imaging and developed targeted NIR fluorophores. Further biological evaluation is progressing with the eventual aim of developing a pre-clinical model for NIR FGS in oncology.
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Zhou, Xiaobo. "Design, synthesis and sensing properties of chiral amine-based fluorescent probes". HKBU Institutional Repository, 2012. https://repository.hkbu.edu.hk/etd_ra/1442.

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Elder, A. D. "Quantitative fluorescence microscopy". Thesis, University of Cambridge, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.598801.

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The work presented here improves the level of quantification achievable with fluorescence microscopy by integrating novel technologies and developing new experimental and theoretical methodologies. Initial work focused on the use of fluorescence microscopy for the quantification of molecular interactions in living cells. This resulted in the development of an analysis routine for the quantification of Förster resonance energy transfer (FRET) by intensity-based sensitised acceptor emission measurements. The developed technique enabled quantification of the strength of interaction as well as the relative stoichiometry of free and bound fluorophores. The work culminated in the dynamic measurement of the cyclin – cyclin dependent kinase interaction through the course of the cell cycle. To improve the flexibility of microscopy techniques, a confocal microscopy system was designed and built which used novel fibre-based supercontinuum illumination technique and a prism-based spectrometer to provide wavelength resolved measurements. The multiparametric imaging approach which this system enabled was shown to aid in the quantification of complex systems. The remainder of this thesis considers the development of new frequency-domain fluorescence lifetime imaging microscopy (FD-FLIM) techniques. The advantages of lifetime imaging techniques were illustrated through their application to quantitative chemical analysis in microfluidic devices. Novel illumination technology was integrated into FD-FLIM systems; both in the form of inexpensive light emitting diodes and fibre-based supercontinuum technology. An in-depth theoretical analysis permitted the development of systems with much improved photon economy. Using extensions of the AB analysis technique, multicomponent lifetime data could be accurately quantified. finally, a new experimental technique was implemented, termed ø2FLIM, which enabled the rapid acquisition of alias-free fluorescence lifetime data.
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Libri sul tema "Fluorescence"

1

Slavík, Jan, a cura di. Fluorescence Microscopy and Fluorescent Probes. Boston, MA: Springer US, 1996. http://dx.doi.org/10.1007/978-1-4899-1866-6.

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D, Slavík Jan Ph, e Conference on Fluorescence Microscopy and Fluorescent Probes (1995 : Prague, Czech Republic), a cura di. Fluorescence microscopy and fluorescent probes. New York: Plenum Press, 1996.

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Deatrick, Elizabeth. Fluorescence. [Chevy Chase, MD]: The author, 2017.

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B, Thompson Richard, a cura di. Fluorescence sensors and biosensors. Boca Raton, FL: Taylor&Francis, 2005.

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Kubitscheck, Ulrich, a cura di. Fluorescence Microscopy. Weinheim, Germany: Wiley-VCH Verlag GmbH & Co. KGaA, 2017. http://dx.doi.org/10.1002/9783527687732.

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Kubitscheck, Ulrich, a cura di. Fluorescence Microscopy. Weinheim, Germany: Wiley-VCH Verlag GmbH & Co. KGaA, 2013. http://dx.doi.org/10.1002/9783527671595.

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Wolfbeis, Otto S., a cura di. Fluorescence Spectroscopy. Berlin, Heidelberg: Springer Berlin Heidelberg, 1993. http://dx.doi.org/10.1007/978-3-642-77372-3.

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Valeur, Bernard, e Mário Nuno Berberan-Santos. Molecular Fluorescence. Weinheim, Germany: Wiley-VCH Verlag GmbH & Co. KGaA, 2012. http://dx.doi.org/10.1002/9783527650002.

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Herman, B. Fluorescence microscopy. 2a ed. Singapore: BIOS, 1998.

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Dick, Jennifer K. Fluorescence: Poems. Athens: University of Georgia Press, 2004.

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Capitoli di libri sul tema "Fluorescence"

1

Gerritsen, Hans C. "Confocal Fluorescence Lifetime Imaging". In Fluorescence Microscopy and Fluorescent Probes, 35–46. Boston, MA: Springer US, 1996. http://dx.doi.org/10.1007/978-1-4899-1866-6_3.

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Cimprich, Petr, e Jan Slavík. "Artifacts in Fluorescence Ratio Imaging". In Fluorescence Microscopy and Fluorescent Probes, 119–23. Boston, MA: Springer US, 1996. http://dx.doi.org/10.1007/978-1-4899-1866-6_16.

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O’Connor, José-Enrique. "Flow Cytometry versus Fluorescence Microscopy". In Fluorescence Microscopy and Fluorescent Probes, 61–66. Boston, MA: Springer US, 1996. http://dx.doi.org/10.1007/978-1-4899-1866-6_6.

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Gundlach, Heinz. "Multichannel Fluorescence Microscopy and Digital Imaging - On the Exciting Developments in Fluorescence Microscopy". In Fluorescence Microscopy and Fluorescent Probes, 67–70. Boston, MA: Springer US, 1996. http://dx.doi.org/10.1007/978-1-4899-1866-6_7.

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Herman, Brian. "Fluorescence Microscopy: State of the Art". In Fluorescence Microscopy and Fluorescent Probes, 1–14. Boston, MA: Springer US, 1996. http://dx.doi.org/10.1007/978-1-4899-1866-6_1.

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Tengholm, Anders, e Eva Grapengiesser. "Disappearance of Cytoplasmic Ca2+ Oscillations is a Sensitive Indicator of Photodamage in Pancreatic β-Cells". In Fluorescence Microscopy and Fluorescent Probes, 85–89. Boston, MA: Springer US, 1996. http://dx.doi.org/10.1007/978-1-4899-1866-6_10.

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7

Cimprich, Petr, e Jan Slavík. "Distribution of Individual Cytoplasmic pH Values in a Cell Suspension". In Fluorescence Microscopy and Fluorescent Probes, 91–93. Boston, MA: Springer US, 1996. http://dx.doi.org/10.1007/978-1-4899-1866-6_11.

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8

Gregor, Martin, Jan Tachezy e Jan Slavík. "The Effect of Lysosomal pH on Lactoferrin-Dependent Iron Uptake in Tritrichomonas foetus". In Fluorescence Microscopy and Fluorescent Probes, 95–99. Boston, MA: Springer US, 1996. http://dx.doi.org/10.1007/978-1-4899-1866-6_12.

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9

Opitz, N., T. Porwol, E. Merten e H. Acker. "On the Protein-Error of the Calcium-Sensitive Fluorescent Indicator Fura-Red". In Fluorescence Microscopy and Fluorescent Probes, 101–6. Boston, MA: Springer US, 1996. http://dx.doi.org/10.1007/978-1-4899-1866-6_13.

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10

Opitz, N., T. Porwol, E. Merten e H. Acker. "Cytoplasmic Ion Imaging: Evidence for Intracellular Calibration Heterogeneities of Ion-Sensitive Fluoroprobes". In Fluorescence Microscopy and Fluorescent Probes, 107–12. Boston, MA: Springer US, 1996. http://dx.doi.org/10.1007/978-1-4899-1866-6_14.

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Atti di convegni sul tema "Fluorescence"

1

Abbey, Emma, Oren Katz, Travis Ferguson, Sarah-Johanna Klose, Chris Pruefert e Hans-Peter Loock. "Applications of Hadamard-Multiplexed Fluorescence Imaging using a Hyperspectral Camera". In CLEO: Applications and Technology, AM4A.2. Washington, D.C.: Optica Publishing Group, 2024. http://dx.doi.org/10.1364/cleo_at.2024.am4a.2.

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Abstract (sommario):
We present a hyperspectral fluorescence imaging system based on a Hadamard-multiplexed light source for monitoring spatially inhomogeneous reactions through time and correctly identifying fluorescent components in complex mixtures.
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2

Armstrong, R. L., J. G. Xie, T. E. Ruskgauer e R. G. Pinnick. "Energy transfer lasing from dye-doped microdroplets seeded with fluorescent sol". In OSA Annual Meeting. Washington, D.C.: Optica Publishing Group, 1992. http://dx.doi.org/10.1364/oam.1992.tug2.

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We report observations of lasing and fluorescence emission from liquid microdroplets of fluorescein 548 dye in ethanol, seeded with submicrometer sized fluorescent sol. An incident pump laser generates droplet fluo­rescein lasing or fluorescene, which in turn excites either fluorescene or lasing in the sol. All emissions are at wavelengths corresponding to morphology dependent reso­nances (MDR's) of the droplet. Studies of the dependence of these emissions on pump laser intensity and sol concentration suggest they are driven by enhanced radiative energy transfer that occurs when fluorescein lasing or fluorescence emission couples to MDR's of the droplet microcavity. Other noteworthy findings include the absence of sol emission for larger sol and the presence of sol emission, even without any observable fluorescein emission, for smaller sol.
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Maruoka, Shoji, Yohei Mitsui, Shinpei Okawa, Yoko Hoshi e Yukio Yamada. "Measurement of Fluorescence Properties in Light Scattering Medium". In ASME/JSME 2011 8th Thermal Engineering Joint Conference. ASMEDC, 2011. http://dx.doi.org/10.1115/ajtec2011-44458.

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The purpose of this study is to measure the fluorescence properties of Indocyaninegreen (ICG) which is a fluorescence dye to be used as a fluorescence probe for the use of fluorescence imaging in biomedical applications. The fluorescence molecular imaging is expected to solve the issues in preclinical studies which require a lot of time, labors and sacrificed animals. Information of living body can be obtained by measuring the fluorescent properties of the probe in biological media. The absorption and emission spectra and the lifetime of ICG in non-scattering and scattering media were measured in this study. ICG was dissolved in water, in plasma, in Intralipid, and in a mixture of plasma and Intralipid to simulate the environment in living tissues. The absorption and emission spectra were measured using a fluorescence spectrophotometer. The fluorescence lifetimes were measured using a time-resolved measurement method. Results suggest that the fluorescent properties are affected by the reaction between ICG and biological tissues.
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Deliolanis, Nikolaos C., Thomas Wurdinger, Bakhos Tannous e Vasilis Ntziachristos. "Fluorescence Tomography of Red-shifted Fluorescent Proteins". In Biomedical Optics. Washington, D.C.: OSA, 2010. http://dx.doi.org/10.1364/biomed.2010.btud4.

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5

Kawata, Satoshi, e Rieko Arimoto. "Laser-scan fluorescence microscope with annular excitation optics". In OSA Annual Meeting. Washington, D.C.: Optica Publishing Group, 1990. http://dx.doi.org/10.1364/oam.1990.mpp1.

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We discuss the three-dimensional imaging characteristics of a laser-scan fluorescence microscope with an annular pupil in the excitation optics. As is well known, the use of an annular pupil in a conventional incoherent imaging system increases the depth of focus and the lateral resolution as compared with the use of a circular pupil.1,2 However, an annular pupil has not been practically used for fluorescence microscopy because it stops a large amount of the fluorescent light arriving at the objective lens. In the case of a laser-scan fluorescence microscope, we may place an annular pupil in the excitation optics rather than in the imaging optics. In this case, we do not waste fluorescent light because the objective-lens pupil is fully open.
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Aborode, Abdullahi. "Light Sheet Fluorescence Microscopy". In Virtual 12th Light Sheet Fluorescence Microscopy Conference 2020. Royal Microscopical Society, 2020. http://dx.doi.org/10.22443/rms.lsfm2020.8.

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Wang, Yao-Nan, Jik Chang Leong, Chin-Lung Chang, Chang-Hsien Tai, Chien-Hsiung Tsai, Lung-Ming Fu e Jr-Ming Miao. "On-Chip Particle Differentiation Utilizing Forward Scattered Light and Fluorescence Light With Passive Focusing". In ASME 2009 Second International Conference on Micro/Nanoscale Heat and Mass Transfer. ASMEDC, 2009. http://dx.doi.org/10.1115/mnhmt2009-18298.

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In this paper, a microflow cytometer for particles/cells passive focusing, fluorescence detection, and sizing is demonstrated with a convergent geometry of 50 μm detection gate channel and a pair of external optic fibers on poly-dimethylsiloxane (PDMS) microchip. A laser (635 nm) is used for fluorescent excitation. The amplitudes of the backward fluorescence signal and forward scattered light signal indicate the corresponding fluorescent intensity and size of the detected fluorescent particles and labeled white blood cells, respectively. In the experiment, the uniform fluorescent light intensity was measured by focusing the fluorescent particles in a 200×50×30μm detection gate channel. However, the emitted fluorescence and forward scattered light intensity of different size particles/cells are also measured simultaneously by external optic fibers.
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Paithankar, D. Y., e E. M. Sevick-Muraca. "Fluorescence lifetime imaging with frequency-domain photon migration measurement". In Biomedical Optical Spectroscopy and Diagnostics. Washington, D.C.: Optica Publishing Group, 2006. http://dx.doi.org/10.1364/bosd.1996.fg3.

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The feasibility of employing fluorescent contrast agents to perform optical imaging in tissues and other scattering media has been examined through computational studies. Fluorescence lifetime and yield can give crucial information about local metabolite concentration or environmental conditions within tissues. This information can be employed towards disease detection, diagnosis, and treatment if non- invasively quantitated from re-emitted optical signals. However, the problem of inverse image reconstruction of fluorescence yield and lifetime is complicated due to the highly scattering nature of the tissue. In this work, a light propagation model employing the diffusion equation is used to account for the scattering of both the excitation and fluorescent light. Simulated measurements of frequency-domain parameters of fluorescent modulated AC intensity and phase-lag are used as inputs to an inverse image reconstruction algorithm which employs the diffusion model to relate frequency-domain measurements resulting from a modulated input at the phantom periphery. In the inverse image reconstruction algorithm, we employ a Newton-Raphson technique combined with Marquardt algorithm to converge upon the fluorescent properties within the medium. The successful reconstruction of both the fluorescence yield and lifetime in the case of heterogeneous fluorophore distribution within a scattering medium has been demonstrated without a priori information or without the necessity of obtaining "absence" images.
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Maier, John S., Albert E. Cerussi, Sergio Fantini, Maria Angela Franceschini e Enrico Gratton. "Quantitative Fluorescence in Tissue-Like Media". In Biomedical Optical Spectroscopy and Diagnostics. Washington, D.C.: Optica Publishing Group, 2006. http://dx.doi.org/10.1364/bosd.1996.fg6.

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Abstract (sommario):
We present a study focused on quantitative fluorescence in tissues at near-infrared wavelengths. We demonstrate the quantitative description of fluorescence in tissue-like media provided by a model derived from diffusion theory. Based on this model we conclude that the spatial distribution of the fluorescent light does not depend on the lifetime of the probe. We also show that lifetime and quantum yield or probe concentration can be determined in a highly scattering medium using frequency-domain techniques, without the use of a reference probe.
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Thompson, Richard B., e Lydia M. Vallarino. "Novel Fluorescent Label For Time-Resolved Fluorescence Immunoassay". In 1988 Los Angeles Symposium--O-E/LASE '88, a cura di Joseph R. Lakowicz. SPIE, 1988. http://dx.doi.org/10.1117/12.945423.

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Rapporti di organizzazioni sul tema "Fluorescence"

1

Hargis, P. J. Jr, B. L. Preppernau e B. P. Aragon. Ultraviolet fluorescence monitor. Office of Scientific and Technical Information (OSTI), maggio 1997. http://dx.doi.org/10.2172/481480.

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2

Allison, S. W. Fluorescence Rise Time Measurements for High Temperature Fluorescence-Based Thermometry. Office of Scientific and Technical Information (OSTI), marzo 2005. http://dx.doi.org/10.2172/885970.

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3

DeRose, Paul C. Standard guide to fluorescence :. Gaithersburg, MD: National Institute of Standards and Technology, 2007. http://dx.doi.org/10.6028/nist.ir.7458.

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Haugland, R. P. Fluorescence-detected DNA sequencing. Office of Scientific and Technical Information (OSTI), gennaio 1990. http://dx.doi.org/10.2172/5619036.

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5

Deng, Chun, Zhenyu Zhang, Zhi Guo, Hengduo Qi, Yang Liu, Haimin Xiao e Xiaojun Li. Assessment of intraoperative use of indocyanine green fluorescence imaging on the number of lymph node dissection during minimally invasive gastrectomy: a systematic review and meta-analysis. INPLASY - International Platform of Registered Systematic Review and Meta-analysis Protocols, novembre 2021. http://dx.doi.org/10.37766/inplasy2021.11.0062.

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Abstract (sommario):
Review question / Objective: Whether is indocyanine green fluorescence imaging-guided lymphadenectomy feasible to improve the number of lymph node dissections during radical gastrectomy in patients with gastric cancer undergoing curative resection? Condition being studied: Gastric cancer was the sixth most common malignant tumor and the fourth leading cause of cancer-related death in the world. Radical lymphadenectomy was a standard procedure in radical gastrectomy for gastric cancer. The retrieval of more lymph nodes was beneficial for improving the accuracy of tumor staging and the long-term survival of patients with gastric cancer. Indocyanine green(ICG) near-infrared fluorescent imaging has been found to provide surgeons with effective visualization of the lymphatic anatomy. As a new surgical navigation technique, ICG near-infrared fluorescent imaging was a hot spot and had already demonstrated promising results in the localization of lymph nodes during surgery in patients with breast cancer, non–small cell lung cancer, and gastric cancer. In addition, ICG had increasingly been reported in the localization of tumor, lymph node dissection, and the evaluation of anastomotic blood supply during radical gastrectomy for gastric cancer. However, it remained unclear whether ICG fluorescence imaging would assist surgeons in performing safe and sufficient lymphadenectomy.
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Mathies, R. A., e A. N. Glazer. Ultrasensitive fluorescence detection of DNA. Office of Scientific and Technical Information (OSTI), gennaio 1992. http://dx.doi.org/10.2172/7176600.

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Mathies, R. A., e A. N. Glazer. Ultrasensitive fluorescence detection of DNA. Office of Scientific and Technical Information (OSTI), gennaio 1991. http://dx.doi.org/10.2172/5827344.

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8

Guo, Yixing. Fluorescence Detection of Biological Thiols. Portland State University Library, gennaio 2000. http://dx.doi.org/10.15760/etd.586.

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9

Scalettar, Bethe A. Fluorescence spectroscopic studies of DNA dynamics. Office of Scientific and Technical Information (OSTI), aprile 1987. http://dx.doi.org/10.2172/10150990.

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Grudberg, Peter Matthew. HIgh Rate X-ray Fluorescence Detector. Office of Scientific and Technical Information (OSTI), aprile 2013. http://dx.doi.org/10.2172/1079831.

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