Bibliografie tematiche / Equid herpesvirus

Letteratura scientifica selezionata sul tema "Equid herpesvirus"

Autore: Grafiati

Pubblicato: 22 giugno 2024

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Articoli di riviste sul tema "Equid herpesvirus":

Vengust, Modest, Xin Wen e Dorothee Bienzle. "Herpesvirus-Associated Neurological Disease in a Donkey". Journal of Veterinary Diagnostic Investigation 20, n. 6 (novembre 2008): 820–23. http://dx.doi.org/10.1177/104063870802000620.

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A 4-year-old donkey was evaluated for progressive neurological abnormalities consisting of depression, stupor, weakness, and recumbency. Diagnostic evaluation for viral involvement identified an asinine herpesvirus in DNA extracted from deep pharyngeal swabs. Specific primers were designed based on comparison with equine herpesviral DNA polymerase sequences and yielded an 875-base pair product from the donkey. This sequence had complete identity with short sequences of asinine herpesvirus previously identified in donkeys with interstitial pneumonia. Amino acid analysis of the entire sequence indicated high similarity with Equid herpesvirus 7 (91%), Zebra herpesvirus 1 (90%), and Equid herpesvirus 2 (89%). With supportive treatment and physical therapy, the donkey gradually recovered over 5 days of hospitalization and returned to normal function. The current case illustrates the potential of a novel asinine herpesvirus to induce neurological disease in donkeys and provides a large viral sequence allowing confident assignment of this virus to the subfamily Gammaherpesvirinae.

Vengust, Modest, John D. Baird, Tony van Dreumel, Cameron Ackerley e Dorothee Bienzle. "Equid Herpesvirus 2-Associated Oral and Esophageal Ulceration in a Foal". Journal of Veterinary Diagnostic Investigation 20, n. 6 (novembre 2008): 811–15. http://dx.doi.org/10.1177/104063870802000618.

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A case of a 1-month-old Thoroughbred foal with dysphagia, salivation, pyrexia, oral mucosal pustules, and esophageal ulceration is reported. Swabs from the ulcerated lesions yielded Equid herpesvirus 2 (EHV-2) in virus isolation assays, and histopathology of a biopsy from the esophageal lesion identified nuclear inclusions suggestive of herpesviruses. Immunohistochemical staining with antibodies specific for EHV-2 was positive for epithelial cells in the vicinity of the ulcer but not in more distant mucosa. Electron microscopic evaluation of the biopsy showed herpesviral particles in epithelial cells. The foal recovered over 5 days of supportive and gastroprotective therapy, and the esophageal ulcers healed. Serology and immunohistochemistry indicated that this foal likely had lesions associated with EHV-2 and not EHV-1, −4, or −5.

Stokol, Tracy, Wee Ming Yeo, Deborah Burnett, Nicole DeAngelis, Teng Huang, Nikolaus Osterrieder e James Catalfamo. "Equid Herpesvirus Type 1 Activates Platelets". PLOS ONE 10, n. 4 (23 aprile 2015): e0122640. http://dx.doi.org/10.1371/journal.pone.0122640.

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INAZU, Mamiko, Osamu TSUHA, Rikio KIRISAWA, Yoshimi KAWAKAMI e Hiroshi IWAI. "Equid Herpesvirus 1 Infection in Mice." Journal of Veterinary Medical Science 55, n. 1 (1993): 119–21. http://dx.doi.org/10.1292/jvms.55.119.

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Osińska, E., A. Golke, A. Słońska, J. Cymerys, M. W. Bańbura e T. Dzieciątkowski. "HybProbes-based real-time PCR assay for rapid detection of equine herpesvirus type 2 DNA". Polish Journal of Veterinary Sciences 15, n. 3 (1 ottobre 2012): 411–16. http://dx.doi.org/10.2478/v10181-012-0064-9.

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Abstract Equid herpesvirus type 2 (EHV-2) together with equid herpesvirus type 5 are members of Gammaherpesvirinae subfamily, genus Rhadinovirus. EHV-2 is one of major agents causing diseases of horses common worldwide. A possible role of EHV-2 in reactivating latent equid herpesvirus type-1 has been suggested, because reactivation of latent EHV-1 was always accompanied by EHV-2 replication. Variety techniques, including cell culture, PCR and its modifications, have been used to diagnose EHV-2 infections. The aim of this study was to develop, optimize and determine specificity of real-time PCR (qPCR) for EHV-2 DNA detection using HybProbesR chemistry and to evaluate clinical samples with this method. The analytical sensitivity of assay was tested using serial dilutions of viral DNA in range between 70 and 7x105 copies/ml. The limit of detection (LOD) was calculated using probit analysis and was determined as 56 copies/ml. In further studies 20 different clinical samples were tested for the presence of EHV-2. Described in-house qPCR method detected viral DNA in 5 of 20 specimens used. The results of this work show that developed HybProbes-based real-time PCR assay is very reliable and valuable for detection and quantification of equid herpesvirus type 2 DNA in different clinical samples. The high level of sensitivity, accuracy and rapidity provided by the LightCycler 2.0 instrument are favorable for the use of this system in the detection of EHV-2 DNA in veterinary virology.

Abdel-Rady, A., I. Abd El-Rahim, S. Gad El-Rab Abd El-Hameed e S. Malek. "Clinical and Molecular Epidemiological Study on Herpesviruses Infection among Equid Populations in Upper Egypt". Journal of the Hellenic Veterinary Medical Society 73, n. 4 (21 gennaio 2023): 4689–872. http://dx.doi.org/10.12681/jhvms.28144.

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The present study was carried out to record the clinical signs of equine herpesviruses (EHVs) infection and to detect the prevalence of EHVs infection among working equids in different provinces of Egypt. A total number of 115 working equids (92 horses and 23 donkeys) were clinically examined and sampled from November 2018 till November 2019 for this study. Two samples were collected from each animal (nasal swab and blood sample) and were subjected to multiplex-PCR to detect the prevalence of different EHVs infection among equids. In the current study, the overall prevalence of EHVs infection among equid populations in Egypt was 80% by using multiplex-PCR. Moreover, the most prevalent equine herpesvirus (EHV) among equids in Upper Egypt was EHV-2 (61.74%), followed by EHV-5 (43.48%), EHV-1 (20%), and EHV-4 (13.04%). The recorded clinical signs of the examined equids harbored EHVs (PCR-positive) can be summarized as follow: a higher percentage was detected among equids with a history of acute onset (59.78%), pyrexia (57.61%), and/or systemic illness (45.65%) with or without respiratory signs (56.52%) and ocular signs (35.87%). Furthermore, 4.35% and 1.09% of EHV-1 PCR-positive equids displayed neurological signs and abortion, respectively.

Stokol, Tracy, Wee Ming Yeo, Deborah Burnett, Nicole DeAngelis, Teng Huang, Nikolaus Osterrieder e James Catalfamo. "Correction: Equid Herpesvirus Type 1 Activates Platelets". PLOS ONE 15, n. 8 (19 agosto 2020): e0237679. http://dx.doi.org/10.1371/journal.pone.0237679.

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Marenzoni, Maria Luisa, Giacomo Coppola, Margherita Maranesi, Fabrizio Passamonti, Katia Cappelli, Stefano Capomaccio, Andrea Verini Supplizi, Etienne Thiry e Mauro Coletti. "Age-dependent prevalence of equid herpesvirus 5 infection". Veterinary Research Communications 34, n. 8 (15 settembre 2010): 703–8. http://dx.doi.org/10.1007/s11259-010-9443-9.

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Canelli, E., G. Manna, G. L. Autorino e P. Cordioli. "Analysis of Italian equid herpesvirus type 1 strains". Journal of Equine Veterinary Science 32, n. 10 (ottobre 2012): S65. http://dx.doi.org/10.1016/j.jevs.2012.08.140.

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Scheurer, Laura, Claudia Bachofen, Isabelle Hardmeier, Julia Lechmann e Angelika Schoster. "Prevalence of Nasal Shedding of Equid Gammaherpesviruses in Healthy Swiss Horses". Viruses 13, n. 9 (25 agosto 2021): 1686. http://dx.doi.org/10.3390/v13091686.

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Equid Gamma herpesvirus (eGHV) infections have been reported worldwide and may be correlated with clinical signs, e.g., affecting the respiratory tract in young horses. eGHV are shed by healthy horses as well as horses with respiratory tract disease. The prevalence in healthy Swiss horses is unknown to date but this data would provide valuable information for causal diagnosis in clinical cases and formulation of biosecurity recommendations. Nasal swabs from 68 healthy horses from 12 Swiss stables and 2 stables near the Swiss border region in Germany were analyzed by panherpes nested PCR. Positive samples were sequenced. A multivariable model was used to determine if sex, age, breed, canton, or stable had a significant effect on the shedding status of each detected eGHV. Overall, the eGHV prevalence was 59% (n = 68); the prevalence for equid herpesvirus-2 (EHV-2), equid herpesvirus-5 (EHV-5) and asinine herpesvirus-5 (AHV-5) was 38%, 12% and 9%, respectively. Co-infections with multiple eGHVs were observed in 25% of the positive samples. The odds of shedding EHV-2 decreased with age (p = 0.01) whereas the odds of shedding AHV-5 increased with age (p = 0.04). Breed, sex, canton, or stable had no significant association with eGHV shedding. As EHV-2 shedding was common in healthy horses a positive PCR result must be interpreted with caution regarding the formulation of biosecurity recommendations and causal diagnosis. As EHV-5 and AHV-5 shedding was less common than EHV-2, a positive test result is more likely to be of clinical relevance. Shedding of multiple eGHV complicates the interpretation of positive test results in a horse.

Più fonti

Tesi sul tema "Equid herpesvirus":

Iqbal, Javid. "Investigations into the regulation of latency of equid herpesvirus 1". Thesis, Royal Veterinary College (University of London), 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.265298.

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Tearle, Jason Paul. "Pathogenesis of equid herpesvirus-1 infection in the male horse". Thesis, Open University, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.363489.

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Sinclair, Robert. "Equid herpesvirus type-1 : antigenic analysis and diagnosis of infection using monoclonal antibodies". Thesis, Open University, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.292324.

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Brown, Lara Jean. "Failure to detect equid herpesvirus type 1 DNA in Thoroughbred placentae and healthy new-born foals". Diss., University of Pretoria, 2009. http://hdl.handle.net/2263/67946.

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Equid alphaherpesvirus 1 (EHV-1) is an economically important virus, associated with respiratory infection, late gestation abortion, neonatal death and myeloencephalopathy in horses. The aim of the present study was to test the hypothesis that EHV-1 is present in the nasopharynx and placentae of neonatal foals in the absence of clinical signs of infection. This would suggest that vertical transmission of virus occurs in inter-epizootic periods: such information could inform foaling management and the potential eradication of the virus by vaccination. Samples were collected from animals resident on a single farm in the Western Cape Province, South Africa, which had not experienced a clinical outbreak of EHV-1 recently. Sterile swab samples from 71 post-partum Thoroughbred mares, their healthy full-term foals and fetal membranes were obtained and assayed for EHV-1 and EHV-4 nucleic acid using a duplex quantitative polymerase chain reaction (qPCR). The null hypothesis for this study was that EHV-1 was not present in the nasopharynx and placentae of new-born, viable and healthy foals. As no EHV-1 or EHV-4 nucleic acid was detected on a duplex EHV-1/EHV-4 qPCR assay from the mare and foal nasal and fetal membrane swabs, the null hypothesis was accepted. It was therefore concluded that there was no detectable EHV-1 and -4 DNA in this population at the time of sampling. It was speculated that this may have been due to the cyclical nature of EHV-1 infections. The inclusion of additional breeding seasons on additional farms would be valuable for future studies.
Dissertation (MSc)--University of Pretoria, 2017.
Production Animal Studies
MSc

Torelli, Camila Souza. "Ocorrência de anticorpos contra o EHV dos tipos 1 e 4 em animais vacinados e não vacinados do Estado de São Paulo". Universidade de São Paulo, 2011. http://www.teses.usp.br/teses/disponiveis/10/10134/tde-04092012-164102/.

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Os herpesvírus equinos do tipo 1 (EHV-1) e do tipo 4 (EHV-4) são considerados os principais agentes infecciosos para a espécie equina. Dentre as doenças causadas por estes agentes, destacam-se a rinopneumonite em animais jovens, o abortamento em fêmeas no terço final da gestação, a mortalidade perinatal em potros e a mieloencefalopatia. Estudos anteriores relatam ampla disseminação do EHV-1 na população eqüina no Estado de São Paulo, entretanto a ocorrência de infecção pelo EHV-4 não possui registro. Devido à similaridade antigênica entre os dois tipos virais, a diferenciação pelos métodos de sorodiagnóstico tradicionais, como a Soroneutralização e a Reação de Fixação de Complemento, não é possível. Assim, este trabalho avaliou, pela primeira vez no Estado de São Paulo, através de um teste de ELISA indireto que emprega uma região da glicoproteína G para diferenciar o EHV-1 do EHV-4 (iELISAgG),a presença de anticorpos específicos para os dois tipos de herpesvírus equino em 512 animais de 20 municípios de 8 mesoregiões do Estado de São Paulo, dentre equinos, muares e asininos, de ambos os sexos, diferentes faixas etárias, vacinados e não vacinados. As mesmas amostras foram testadas para o EHV através do teste de soroneutralização, tradicionalmente empregado para a pesquisa de anticorpos contra o vírus. Os resultados obtidos com a soroneutralização revelam 205/512 (40,03%) animais soropositivos. Através do teste de ELISA obteve-se 3/512 (0,59%) animais positivos para o EHV-1, 347/512 (67,77%) animais positivos para o EHV-4 e 108/512 (21,09%) animais positivos para ambos. O grupo de animais não vacinados apresentou 127/352 (36,07%) soropositivos pelo teste de soroneutralização; enquanto 4/352 (1,14%) foram positivos para o EHV-1, 237/352 (67,33%) foram positivos para o EHV-4 e 69/352 (19,6%) foram positivos para ambos, pelo teste de ELISA. O grupo de animais vacinados apresentou 78/160 (48,75%) soropositivos pelo teste de soroneutralização; enquanto 1/160 (0,63%) foram positivos para o EHV-1, 112/160 (70%) foram positivos para o EHV-4 e 37/160 (23,13%) foram positivos para ambos, pelo teste de ELISA. Os resultados sugerem baixa circulação de EHV-1 e alta circulação de EHV-4, de acordo com os resultados encontrados nos animais não vacinados. A análise de correlação entre os dois testes empregados mostrou baixa concordância.
The equine herpesvirus type 1 (EHV-1) and type 4 (EHV-4) are considered the major infectious agents for the equine species. Among the diseases caused by these agents, we highlight the rinopneumonite in young animals, abortion in females in the final third of pregnancy, perinatal mortality in foals and encefalopathy. Previous studies have reported wide spread of EHV-1 equine population in the State of São Paulo, however the occurrence of infection with EHV-4 is not registered. Due to the antigenic similarity between the two virus types, the differential serodiagnosis by traditional methods such as neutralization and complement fixation reaction, it is not possible. Thus, this study evaluated the first time in São Paulo, through an indirect ELISA employing a region of glycoprotein G to differentiate EHV-1 EHV-4 (iELISAgG), the presence of specific antibodies to the two types of equine herpesvirus in 512 animals from 20 municipalities in 8 regions the State of São Paulo, among horses, mules and donkeys of both sexes, different age groups, vaccinated and unvaccinated. The same samples were tested for EHV through the neutralization test, traditionally used for the detection of antibodies against the virus. The results obtained with the neutralization revealed 205/512 (40.03%) seropositive animals. By ELISA we obtained 3/512 (0.59%) animals positive for EHV-1, 347/512 (67.77%) animals positive for EHV-4 and 108/512 (21.09% ) animals positive for both. The group of unvaccinated animals showed 127/352 (36.07%) HIV-positive by serum neutralization test, while 4/352 (1.14%) were positive for EHV-1, 237/352 (67.33%) were positive for EHV-4 and 69/352 (19.6%) were positive for both ELISA. The group of vaccinated animals showed 78/160 (48.75%) seropositive by neutralization test, while 1 / 160 (0.63%) were positive for EHV-1, 112/160 (70%) were positive for EHV-4 and 37/160 (23.13%) were positive for both ELISA. The results suggest low circulation of EHV-1 and high circulation of EHV-4 according to the results found in unvaccinated animals. The correlation analysis between the two tests employed showed poor agreement

Fritsche, Ann-Kathrin [Verfasser]. "Virological and molecular biological characterization of Equid Herpesvirus 1 (EHV-1) isolates from Germany / Ann-Kathrin Fritsche". Berlin : Freie Universität Berlin, 2015. http://d-nb.info/1078017506/34.

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Picard, J. A. "Respiratory pathogens in thoroughbred foals up to one year of age on a stud farm in South Africa". Diss., University of Pretoria, 2005. http://hdl.handle.net/2263/22867.

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The project was undertaken to monitor a group of 30 foals on a farm both clinically and microbiologically from birth until one year of age, to determine the aetiology of upper respiratory tract infections and to establish immune profiles of some of the known respiratory viral pathogens. One to two months prior to the birth of their foals, blood for serology was collected from the mares. The same specimens were collected from the foals just after birth, prior to suckling and a day after suckling. Thereafter the foals were examined monthly for the presence of respiratory disease and specimens taken. The following specimens were collected from each foal: three nasopharyngeal swabs, (one for virus isolation, one for bacteria and fungus isolation, and one for mycoplasma isolation) and blood that was allowed to clot. Blood was collected in heparin from sick foals with elevated rectal temperatures. Virus isolation was done on roller tube cultures of equine embryonic lung (EEL), Vero cells and rabbit kidney 13 (RK13) cells. The bacteria (including mycoplasmas) and fungi were cultured from the swabs and identified using a variety of traditional methods. The serum neutralization test (SNT) was used to detect antibodies to equid herpesvirus 1 (EHV-1), equid herpesvirus 4 (EHV-4), equine rhinovirus 1 (ERV-1), equine rhinovirus 2 (ERV-2) and equine adenovirus 1 (EAdV-1). The complement fixation test (CFT) was used to detect antibodies to EHV-1 and EHV-4 and the haemagglutination inhibition test (HIT) antibodies to equine influenzavirus (EIV). Only EHV-4 was cultured from the nasopharyngeal swabs of nine foals when they were 5 to 6 months of age and from one foal two months later. A wide variety of bacteria and fungi were cultured and it was established that coagulase-negative staphylococci, viridans streptococci, Moraxella spp. and Flavobacterium spp. predominated in most of the samples. Several potential bacterial pathogens were isolated but the most common were Streptococcus equi subsp. zooepidemicus, Actinobacillus equuland Staphylococcus aureus. Colostrum-derived antibodies were detected for all the viruses in all but two of the foals. It was found that the foals had similar or slightly higher titres than their mothers. The levels declined in direct proportion to what they initially were and were depleted by the time the foals were 2 to 7 months of age. Antibodies to natural infection was detected to EHV-4, ERV-2 and EAdV-1. A rise in antibody titres occurred when the foals were 5 to 6 months of age, two months later and when they were one year of age. Antibodies resulting from immunization was detected to EHV-1, EHV-4 and EIV. It was established that the most important virus causing upper respiratory tract disease of the foals from 5 to 12 months of age was EHV-1 with EAdV-1 playing a minor role. These viruses caused repeated bouts of infection with a two to five months interval. Streptococcus equi subsp. zooepidemicus was considered to be the most important secondary pathogen. Prior to this period most of the foals were healthy with only a few suffering from upper respiratory disease. The aetiology was not determined in these cases, but based on the bacteriology results, it was suspected that some of them were suffering from bacterial infections.
Dissertation (MSc (Veterinary Science))--University of Pretoria, 2005.
Veterinary Tropical Diseases
unrestricted

Carvalho, Rodrigo Franco. "Caracterização genomica de isolados brasileiros do herpesvirus equino do tipo 1". [s.n.], 2005. http://repositorio.unicamp.br/jspui/handle/REPOSIP/316620.

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Orientador: Clarice Weis Arns
Tese (doutorado) - Universidade Estadual de Campinas, Instituto de Biologia
Made available in DSpace on 2018-08-05T13:06:28Z (GMT). No. of bitstreams: 1 Carvalho_RodrigoFranco_D.pdf: 1262824 bytes, checksum: 9c044e92e7146ea5bb090e025e656061 (MD5) Previous issue date: 2005
Resumo: O herpesvírus eqüino do tipo 1 (EHV-1) é um membro da subfamília Alfaherpesvirinae, implicado no surgimento de distúrbios respiratórios, reprodutivos e nervosos em cavalos. A principal forma de contaminação dos animais é através do contato direto com secreções contaminadas pelo vírus. No eqüino, a disseminação do vírus ocorre pela transposição da infecção respiratória a outros órgãos e sistemas através da corrente sanguínea. Pouco se sabe sobre a ocorrência do EHV-1 no Brasil. Dessa forma, este estudo teve por objetivo o isolamento do EHV-1 a partir de material biológico e produção e análise de dados moleculares de isolados brasileiros de EHV-1. Durante este estudo, foi realizado o isolamento de uma amostra de EHV-1 a partir da inoculação de material clínico em células de derme eqüina (ED). Este isolado foi diagnosticado como EHV-1 através da reação em cadeia da polimerase (PCR) para o gene da timidina quinase (tk). Neste trabalho, foram também realizados os seqüenciamentos de fragmentos de PCR derivados do isolado aqui descrito, de uma outra amostra brasileira de EHV-1 e de duas amostras estrangeiras do vírus para análise filogenética. A análise comparativa entre seqüências permitiu inferências sobre o nível de divergência entre os vírus estudados, além da listagem de seqüências regulatórias para atividade gênica em um sítio do genoma localizado próximo ao gene tk. Na região genômica reportada foram contextualizadas ao menos três genes (ORF 38, ORF 37 e ORF 36). Os dados levantados com o seqüenciamento de amostras de EHV-1 de origens geográficas distintas (Brasil, Europa e América do Norte) não mostraram divergências, o que pode estar associado a um processo seletivo constritivo, que impediria a fixação de novas mutações naquela região. A ausência de divergências também pode estar associada à importância dessa região na regulação gênica do EHV-1. Também é um indicativo para a fidelidade dos mecanismos de replicação envolvidos na síntese do DNA viral, o que sugere a importância da região estudada na regulação da expressão gênica do EHV-1
Abstract: Equine Herpesvirus Type 1 (EHV-1) is a member of Alphaherpesvirinae subfamily implicated with abortions, respiratory and neurological disturbs in horses. The principal mode of viral transmission is through close contact virus-containing secretions of infected horses. Systemic pathogenesis in which this virus is implicated combines primary respiratory infection and spread of viral particles through the circulatory/lymphatic system. Until today, there are only few studies involving the isolation of this virus in Brazil. Thus, the main goal of this study was the isolation of EHV-1 from biological material and the production and analysis of molecular data derived from Brazilian EHV-1 isolates. Clinical samples were screened by inoculation into Equine Dermis (ED) cells monolayers, searching for the characteristic citopathic effect produced by EHV-1. Inoculation of one tissue sample has presented a suggestive citopathic effect. Re-inoculation of the original tissue homogenate in a second, independent experiment reproduced the same positive result. Following these observations, infection agent diagnostic was done by PCR for thymidine kinase (tk) gene. The results demonstrated that sample was EHV-1 positive. In this work, it was done either the sequencing of PCR fragments derived from two Brazilian and two foreign samples of EHV-1 for filogenetic and genomic analyses purposes. It was assigned at least three Open Reading Frames contexts (ORF 38, ORF 37, ORF 36). The data do not show genetic variation between sequences. The high level of genetic conservation for this region, despite the distinct geographic origins (Brazil, Europe and North America) of EHV-1 samples studied, indicates a strong selection process against the fixation of new mutations. It also highlights a high level of fidelity for DNA replication and strongly suggests the importance of the studied region for EHV-1 gene regulation
Doutorado
Microbiologia
Doutor em Genetica e Biologia Molecular

Seeber, Peter Andreas [Verfasser]. "Equid herpesvirus infections in zebras (Equus sp.): host physiology and non-invasive detection of virus shedding / Peter Andreas Seeber". Berlin : Freie Universität Berlin, 2019. http://d-nb.info/1177152622/34.

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Dunuwille, Saranajith Wangisa. "MODULATION OF INFLAMMATORY CYTOKINE, CHEMOKINE, AND TOLL-LIKE RECEPTOR GENES AND TRANSCRIPTOME ANALYSIS OF EQUINE ENDOTHELIAL CELLS FOLLOWING INFECTION WITH EQUID HERPESVIRUS-1, AND EQUINE ARTERITIS VIRUS". UKnowledge, 2019. https://uknowledge.uky.edu/gluck_etds/44.

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Abstract (sommario):

EHV-1 is a double-stranded DNA virus whereas EAV is a positive sense, single-stranded RNA virus. Therefore, genetically, they are very different from one another. However, both these viruses are endotheliotropic and thus, infect and replicates in equine endothelial cells resulting in vasculitis. Vasculitis is central to the pathogenesis of these two viruses. Thus, the main objective of this thesis was to investigate the inflammatory and innate immune responses of EECs that contribute towards the development of vasculitis following infection with EHV-1 and EAV in-vitro. Since proinflammatory cytokines and chemokines produced by endothelial cells play a significant role in the development of vasculitis, we investigated their gene expression as well as secretion. Results from this study showed that the proinflammatory response of EECs induced by EAV is relatively less when compared with the corresponding results from EHV-1 infected EECs. Furthermore, EAV elicits a lower type I interferon response in EECs when compared with EHV-1. Further investigations revealed an active role played by TLR 3 in inducing the proinflammatory response in EHV-1 infected EECs during the first 6 hours of infection but not in EAV infected EECs. Analyzing the whole transcriptome of EHV-1 and EAV infected EECs revealed a complex pattern of gene regulation and cellular pathways related to cellular immune, inflammatory and apoptotic responses. Finally, we investigated host genetic factors associated with EHV-1 induced myeloencephalopathy but found no evidence for a recessive allele influencing the development of EHM following EHV-1 infection for any genetic locus was identified. However, more complex host-pathogen interactions are possible.

Più fonti

Libri sul tema "Equid herpesvirus":

Board, Horserace Betting Levy, a cura di. Codes of practice on contagious equine metritis (CEM), klebsiella pneumoniae, pseudomonas aeruginosa, equine viral arteritis (EVA), equid herpesvirus-1 (EHV-1): Guidelines on strangles. London: Horserace Betting Levy Board, 1996.

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Board, Horserace Betting Levy, a cura di. Abbreviated codes of practice on contagious equine metritis (CEM), klebsiella pneumoniae, pseudomonas aeruginosa, equine viral arteritis (EVA), equid herpesvirus-1 (EHV-1): Guidelines on strangles. London: Horserace Betting Levy Board, 1999.

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Association, Irish Thoroughbred Breeders'. Common code of practice for control of contagious equine metritis and other equine bacterial venereal diseases, and equine viral arteritis for the 1993 covering season in France, Germany, Ireland, Italy and United Kingdom, and code of practice for equid herpesvirus 1 (EHV-1) infection. [s.l.]: Irish Thoroughbred Breeders' Association, 1992.

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Capitoli di libri sul tema "Equid herpesvirus":

Goehring, Lutz S. "Equid Herpesvirus-Associated Myeloencephalopathy". In Equine Neurology, 223–32. Hoboken, NJ: John Wiley & Sons, Inc, 2015. http://dx.doi.org/10.1002/9781118993712.ch18.

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Goehring, Lutz S. "Equid Herpesvirus–Associated Myeloencephalopathy". In Robinson's Current Therapy in Equine Medicine, 387–90. Elsevier, 2015. http://dx.doi.org/10.1016/b978-1-4557-4555-5.00090-x.

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