Tesi sul tema "Effect of nitrogen on"
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Farr, C. R. "Nitrogen Stabilizer Effect on Nitrate Nitrogen Management in Soils". College of Agriculture, University of Arizona (Tucson, AZ), 1987. http://hdl.handle.net/10150/204454.
Testo completoZhou, Maoqian 1961. "Nitrogen fixation by alfalfa as affected by salt stress and nitrogen levels". Thesis, The University of Arizona, 1989. http://hdl.handle.net/10150/277231.
Testo completoHaig, Paul Andrew. "Effect of dietary nitrogen solubility on nitrogen losses from lactating dairy cows". Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp01/MQ43169.pdf.
Testo completoBhogal, Anne. "Effect of long-term nitrogen applications on nitrogen cycling under continuous wheat". Thesis, University of Nottingham, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.294731.
Testo completoSaunders, Eleanor Margaret. "The effect of mineral nitrogen on ectomycorrhizas with special reference to nitrogen deposition". Thesis, University of Sheffield, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.299547.
Testo completoCepeda, Jose de los Angeles 1955. "Nitrogen fixation by alfalfa as affected by osmotic potentials and measured by nitrogen-15 techniques". Thesis, The University of Arizona, 1987. http://hdl.handle.net/10150/276591.
Testo completoIppersiel, Denis. "The effect of foliar nitrogen fertilization on nitrogen distribution, yield and protein quality of forage corn /". Thesis, McGill University, 1986. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=63798.
Testo completoPapadopoulos, Anastasios K. "Nitrogen and moisture distributions under subirrigated soybeans". Thesis, McGill University, 1994. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=55520.
Testo completoYields were measured in terms of number of beans per plant, number of pods per plant, number of beans per pod, and seed protein content at harvest.
Soil samples collected at depths of 30 and 70 cm from the soil surface were analyzed for moisture content and NO$ sb3 sp-$-N and NH$ sb4 sp+$-N concentrations.
The experimental results showed that controlled watertable management increased the yield and decreased soil NO$ sb3 sp-$-N levels. The best results from the watertables tested were found to be at 60 and 80 cm. This is suggested as the range of watertable depths that should be maintained for optimum soybean production.
Denton, Bethany L. "Effect of Orange Peels on Nitrogen Efficiency in Ruminants". The Ohio State University, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=osu1471877758.
Testo completoChoudhury, D. "The effect of honeydew on leaf-litter decomposition, soil non-symbiotic nitrogen fixation and nitrogen mineralization". Thesis, University of Oxford, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.376903.
Testo completoVan, Wijk Kim. "The effect of organic carbon and nitrogen additions on inorganic nitrogen uptake by phytoplankton and bacteria". Master's thesis, University of Cape Town, 1991. http://hdl.handle.net/11427/18342.
Testo completoThis study examines the effects of enrichment with organic carbon and nitrogen on inorganic nitrogen partitioning between phyto- and bacterio- plankton. Strongly preferential uptake of ammonium over nitrate was observed by both the phytoplanktonic and bacterial fractions, with RPINH4 values typically between 1 and 5. The bacterial fraction ( <0.8μm) was found to be responsible for as much as 48-75% of community uptake of ammonium; while the netplanktonic fraction was observed to take up approximately 50% of intact community uptake of nitrate. The addition of amino acids appeared to mediate bacterial competition for ammonium, indicating their preference for DON as a nitrogen source and allowing increased ammonium uptake by the nanoplanktonic fraction. The effect of glucose enrichment was complicated by the presence of protozoans, which appeared to be indirectly responsible for decreased ammonium uptake due to depletion of this substrate in the presence of added glucose. The nanoplankton appeared to be responsible for the least nitrogen uptake with respect to biomass, indicating that they may have been subject to competition pressure from both the bacterial and netplanktonic fractions.
Jackson, Brian A. "Nitrogen mineralization of incorporated bahiagrass sod and its effect on nitrogen uptake and yield of maize". [Gainesville, Fla.] : University of Florida, 2003. http://purl.fcla.edu/fcla/etd/UFE0001441.
Testo completoGreen, Emma Rachel. "The effect of nitrogen deposition on Lowland heath ecosystem". Thesis, Imperial College London, 2006. http://hdl.handle.net/10044/1/11421.
Testo completoForoutan-Pour, Kayhan. "Aspects of barley post-anthesis nitrogen physiology". Thesis, McGill University, 1994. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=22730.
Testo completoTOKOROYAMA, Takayuki, e Noritsugu UMEHARA. "Effect of nitrogen atoms included in CNx coatings on friction sliding against Si3N4 ball in nitrogen gas". IEEE, 2007. http://hdl.handle.net/2237/9456.
Testo completoOttman, Michael J., Duarte E. Diaz, Michael D. Sheedy e Richard W. Ward. "Nitrogen Fertilizer Rate Effect on Forage Sorghum Yield, Quality, and Tissue Nitrogen Concentrations at Maricopa, AZ, 2015". College of Agriculture, University of Arizona (Tucson, AZ), 2017. http://hdl.handle.net/10150/625439.
Testo completoA nitrogen fertilizer study was conducted in order to determine the effect of N rates on forage sorghum yield and quality and to develop tissue testing guidelines for fertilizer application to forage sorghum. The study was conducted at the University of Arizona Maricopa Agricultural center on sandy clay loam soil irrigated using the flood method. Forage sorghum was fertilized with six N rates varying from 0 to 250 lb N/acre in 50 lb N/acre increments. The whole plant, lower stem, and most recently expanded leaf were sampled five times during the growing season and analyzed for N content in order to establish tissue N guidelines for fertilizer application. The plant part that was most sensitive to N fertilizer application and plant N status was lower stem. Leaf and plant N levels were not affected by fertilizer application. The stem nitrate and stem N tests were able to identify N deficient plants very early in the season, long before plant growth was affected by the N deficiency, unlike leaf and plant N. Forage yield at final harvest fitted to a quadratic function was maximized at the 250 lb N/acre N rate. However, the yield increase with any amount of fertilizer did not pay for the cost of the fertilizer and the most economical N rate for yield was no N fertilizer applied at all. In terms of milk per acre, the maximum was achieved at 150 lb N/acre, and the economic optimum in terms of milk was slightly less than this amount of fertilizer.
Morton, Lincoln William. "The role of dietary phenolic compounds in the detoxification of reactive nitrogen species". University of Western Australia. Dept. of Medicine, 2003. http://theses.library.uwa.edu.au/adt-WU2003.0026.
Testo completoAriapour, Azita. "Effect of nitrogen on Fe-Mn-based shape memory alloys". Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape11/PQDD_0002/NQ41396.pdf.
Testo completoKhamees, H. S. "Effect of some ecological factors on nitrogen fixation by cyanobacteria". Thesis, Swansea University, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.637784.
Testo completoReece, William Hugh Hathorn. "The effect of reactive nitrogen intermediates on Leishmania mexicana mexicana". Thesis, University of Glasgow, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.363164.
Testo completoDiallo, Sory. "Effect of genotypes and nitrogen on grain quality of sorghum". Thesis, Kansas State University, 2012. http://hdl.handle.net/2097/14628.
Testo completoDepartment of Agronomy
P.V. Vara Prasad
Sorghum (Sorghum bicolor L. Moench) is cultivated as an important food grain in the semi-arid regions of Africa. Processed grain sorghum is traditionally consumed as porridge, couscous, traditional tô or beer. The quality of such foods is highly dependent upon grain characteristics. Sorghum grain quality traits mainly include kernel hardness, kernel weight, kernel size, protein content and kernel color. Grain quality traits are often influenced by environment, genotypes, fertilizer management and their interaction. The objective of this study was to determine the impact of different levels of nitrogen application (0, 45, and 90 kg ha[superscript]-1) on grain quality of selected sorghum genotypes. The field experiment was conducted at three locations in 2010 (Manhattan, Ottawa, and Hays) and at two locations in 2011 (Manhattan and Ottawa). The experiment was laid in split plot randomized complete bloc design and replicated four times. The main plots were assigned to three N regimes: control (0 kg N ha[superscript]-1), half recommended rate (45 kg N ha[superscript]-1) and recommended rate (90 kg N ha[superscript]-1). The subplots were assigned to twelve genotypes (six hybrids and six inbred lines). Plot size was 6.1 m x 3.0 m with a row spacing of 0.75 m. After harvest, grain quality traits (hardness, weight, diameter and protein content) were evaluated using standard procedures and the data subjected to statistical design using SAS. There were significant effects of genotype for most grain quality traits across both locations in Manhattan. Inbred lines SC35 and SC599 had maximum hardness at all locations while hybrid 95207, had the lowest hardness for all locations. Also, Inbred lines SC35 and Tx340 had maximum protein content at all the locations. While hybrids 95207, 26056, 23012 had the lowest protein content. Genotypes Tx430, SC35, had higher hardness and with higher protein content were classified as high quality. We conclude that application of N (45 or 90 kg ha[superscript]-1) significantly improved grain protein, but not other quality traits. There are opportunities to improve grain protein through fertilizer management and plant breeding.
Gentili, Matilde. "Effect of nitrogen nutrition on yeast ecology and alcoholic fermentation". Master's thesis, ISA-UL, 2016. http://hdl.handle.net/10400.5/12216.
Testo completoWine is an alcoholic beverage obtained from the fermentation of grape juice. Alcoholic fermentation is the main process to obtain the final product but of course other reactions take place during the transformation of grape juice into wine. Yeasts are one of the fundamental microorganisms to realize the alcoholic fermentation, that leads sugars to be transformed in ethanol and to development of other compounds. Yeasts as known are living organisms so they need nutrients for their reproduction and often the lack of some important nutrients can lead to a stuck or slug in their metabolic activity. One of the most important nutrient for yeasts growth is Nitrogen, main component of aminoacids and proteins, that constitute the basic structures of the microorganism.In winemaking, both in the cellar and in the vineyards, the addition of nitrogen under different circumstances is a common practice. Nitrogen seems to influence not only fermentation rate and yeast growth but many of the fermentation product such as ethanol, acetic acid, glycerol, and of course residual sugars and the production of some off flavours,such as hydrogen sulphide. Although each yeast, species and strains,showed different behaviour both in the fermentation rates, growth and production of different metabolites. Not only the quantity and kind of Nitrogen source but also the timing of the addition seem to influence all these aspects in slightly different way between different strain. The nitrogen compounds especially apparently ammino acids showed also a particular relationship with the production of esters of higher alcohols and other compounds but the pattern of ester production is still uncertain. In this study there will be considered the main aspects of nitrogen nutrition, how it affects the yeasts ecology and the different conditions on which it is used and also different strains and species requirements
Horner, Emily Renee. "THE EFFECT OF NITROGEN APPLICATION TIMING ON PLANT AVAILABLE PHOSPHORUS". The Ohio State University, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=osu1211932436.
Testo completoPayne, Kathryn Marie. "ENHANCED EFFICIENCY NITROGEN FORMULATION EFFECT ON GRASS-LEGUME PASTURE PRODUCTIVITY". UKnowledge, 2019. https://uknowledge.uky.edu/pss_etds/113.
Testo completoAl-Rawahy, Salim Ali. "Nitrogen uptake, growth rate and yield of tomatoes under saline conditions". Diss., The University of Arizona, 1989. http://hdl.handle.net/10150/184894.
Testo completoNilsson, Hultén Leo. "Nitrogen Without Oxygen : The effect of ferroalloys added after vacuum treatment on cleanliness of nitrogen-alloyed tool steel". Thesis, KTH, Materialvetenskap, 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-298093.
Testo completoKvävelegerat verktygsstål tillverkas hos Uddeholms AB genom tillsats av kväverika ferrolegeringar efter vakumavgasning, och orenheter som introduceras i detta steg är svåra att avskilja. I den har uppsatsen jämförs två typer av kväverikt ferrokrom varav en i form av stycken och en i form av tråd med pulverkärna. De undersöks i tvärsnitt och totalt 16 prov från fyra charger undersöks med PDA (pulsfördelningsanalys) och systematisk mikroskopering i tvärsnitt. Resultaten från PDA missade mindre inneslutningar av spinell vilka tidigare har visat sig problematiska. Trådformen verkar lovande men fler charger behöver undersökas för att kunna dra en definitiv slutsats.
Tshuma, Takula. "The effect of blood urea nitrogen on reproductive performance of beef heifers on different levels of nitrogen supplementation". Diss., University of Pretoria, 2013. http://hdl.handle.net/2263/36790.
Testo completoDissertation (MMedVet)--University of Pretoria, 2013.
gm2014
Production Animal Studies
Unrestricted
Thaung, Tint Lwin. "Effect of nitrogen fertiliser additions on nitrogen fluxes and plantation productivity in young eucalyptus cloeziana (F. Muell) plantations /". [St. Lucia, Qld.], 2002. http://www.library.uq.edu.au/pdfserve.php?image=thesisabs/absthe16847.pdf.
Testo completoPandeya, Hemant Raj. "Carbon and nitrogen flux dynamics in highly weathered tropical mango soils: Effect of leaf litter and nitrogen fertiliser". Thesis, Queensland University of Technology, 2022. https://eprints.qut.edu.au/232828/1/Hemant%20Raj_Pandeya_Thesis.pdf.
Testo completoMooleki, Siyambango Patrick. "Synchronization of nitrogen availability and plant nitrogen demand, nitrogen and non-nitrogen effects of lentil to subsequent wheat crops". Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2000. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape2/PQDD_0029/NQ63902.pdf.
Testo completoAntunes, Cristiane Leite [UNESP]. "Fertirrigação nitrogenada por gotejamento e época de aplicação foliar de ácido giberélico 'GA IND.3' em alface americana (Lactuca sativa L.)". Universidade Estadual Paulista (UNESP), 2001. http://hdl.handle.net/11449/93828.
Testo completoCom o objetivo de avaliar os efeitos do nitrogênio aplicado ao solo, via fertirrigação por gotejamento, e a aplicação foliar do ácido giberélico (GA3), na nutrição e nos componentes de produção da alface tipo americana, cv. 'Lucy Brown', em cultivo protegido, foi efetuado o presente trabalho. O delineamento experimental utilizado foi o de blocos casualizados em esquema de parcelas subdivididas: quatro doses de nitrogênio (60, 120, 180 e 240 kg de N.ha-1), na parcela, e quatro épocas de aplicação de GA3 (0, 40, 55 e 70 DAS - dias após a semeadura), na subparcela. O ácido giberélico foi aplicado com pulverizador costal de CO2, numa concentração única de 20 mg do ingrediente ativo.L-1 de solução, nas subparcelas nas épocas determinadas. Foram realizadas quatro coletas para avaliação dos parâmetros vegetativos e análise de nutrientes, com intervalos de 15 dias, iniciando-se aos 51 DAS (23 DAT- dias após o transplantio) até 96 DAS (64 DAT). Os trabalhos foram conduzidos na área experimental da Fazenda São Manuel, no município de São Manuel - SP. Não foi observada interação entre a aplicação de nitrogênio e o ácido giberélico nos parâmetros vegetativos avaliados, bem como no teor e acúmulo dos macronutrientes. Contudo, houve influência dos fatores de variação de forma independente nos parâmetros ao longo do ciclo. O nitrogênio é extremamente importante no crescimento e acúmulo de matéria fresca na planta de alface tipo americana e pode ser usado em doses maiores quando há disponibilidade de cálcio e potássio. Tendo sido os melhores resultados alcançados com a dose de 180 kg de N. ha-1. O uso do ácido giberélico (GA3), aos 70 DAS (dias após a semeadura) proporcionou os melhores resultados nos parâmetros relativos ao crescimento. Enquanto a época de aplicação aos 40 DAS, promoveu o desenvolvimento intenso do caule, levando a efeitos... .
With the goal of estimating the nitrogen effects applied on the soil, through fertigation by dripping, and the foliar application of gibberellic acid (GA3), in the nourishment and in the components of Crisphead Lettuce production, cv. 'Lucy Brown', in protected cultivation, this paper was done. The used experimental drawing was made of casual blocks in subdivided portions: four doses de nitrogen (60, 120 180 and 240 kg de N.ha-1), in the portion, and four application times of GA3 (0, 40, 55 e 70 DAS - days after sowing) in the subportion. The gibberellic acid was applied with a costal pulverize machine of CO2, in a unique dose of 20 mg.L-1 of the active ingredient, in the subportions in the specific times. Four collects were done for the evaluation of the vegetative parameters and nutrient analysis, with a 15-days recess, beninning on 51 DAS (23DAT) to 96 DAS (64 DAT). The works were managed in the experimental area of São Manuel Farm, in São Manuel County - SP. It wasn't observed interaction between nitrogen application and the gibberellic acid in the evaluated vegetative characteristics, as well as in the tenor and accumulation of macro-nutrients. However, there was na influence of changing factors in na independent way in the parameters along the cycle. The nitrogen is extremely important in the growth and accumulation of fresh material in the Crisphead lettuce vegetable and it can be used in bigger doses when there is availability of calcium and potassium. These have been the best results got with the dose 180 kg of N.ha-1. The usage of gibberellic acid (GA3), on 70 DAS (days after the sowing) provided the best results in the characteristics related to the growth. As long as the application time on 40 DAS, provided the intense satlk growth, conducing to the depreciatory effects, as the plants arranged in layers, a bigger number of external leaves, and a samller accumulation of fresh... (Complete abstract, click electronic address below).
Heidari, Sharif Abad Hossein. "Variation in the sensitivity of nodulation and nitrogen fixation to nitrate in annual "Medicago" species". Title page, table of contents and abstract only, 1994. http://web4.library.adelaide.edu.au/theses/09PH/09phh465.pdf.
Testo completoYu, Shaobing. "Winter wheat nitrogen management in south coastal British Columbia". Thesis, University of British Columbia, 1990. http://hdl.handle.net/2429/29888.
Testo completoLand and Food Systems, Faculty of
Graduate
Caglia, Stefania. "Nitrogen Removal in the Pilot Plant ITEST (Increased Technology in Sewage Treatment)". Thesis, KTH, Mark- och vattenteknik (flyttat 20130630), 2013. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-171846.
Testo completoHanson, Carter Curtis. "Temporal effect on nitrogen removal in a subsurface flow constructed wetland". Virtual Press, 1996. http://liblink.bsu.edu/uhtbin/catkey/1027117.
Testo completoDepartment of Natural Resources and Environmental Management
Thanapornpoonpong, Sa-nguansak. "Effect of nitrogen fertilizer on nitrogen assimilation and seed quality of amaranth (Amaranthus spp.) and quinoa (Chenopodium quinoa Willd)". Doctoral thesis, [S.l.] : [s.n.], 2004. http://hdl.handle.net/11858/00-1735-0000-0006-AB4D-1.
Testo completoAyub, Najma. "The effect of VAM inoculation on interplant ¹⁵N transfer". Thesis, University of Aberdeen, 1991. http://digitool.abdn.ac.uk/R?func=search-advanced-go&find_code1=WSN&request1=AAIU546184.
Testo completoYarber, Elizabeth Lee. "Assessing the Effect of Nitrogen Sources, Rates and Time of applications on Yield and Quality of Stockpiled Fescue and Tall Fescue Pastures". Thesis, Virginia Tech, 2008. http://hdl.handle.net/10919/33692.
Testo completoMaster of Science
Sivapathasundram, Sudhersha. "The effect of nitrogen starvation on PS2 in the cyanobacterium synechococcus". Thesis, Liverpool John Moores University, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.275823.
Testo completoYoung, Sarah Louise. "Effect of nitrogen fertiliser on bacterial community dynamics in arable soils". Thesis, University of Essex, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.423712.
Testo completoClark, Lee J., e Bryant R. Gardner. "Effect of Nitrogen Fertilizer Application on Cotton Yields, Safford Agricultural Center". College of Agriculture, University of Arizona (Tucson, AZ), 1986. http://hdl.handle.net/10150/219792.
Testo completoA soil depleted of nitrogen by cropping with Sudan grass and removing all the residues was planted to Upland (DP 90) and Pima (S-6) cotton. Nitrogen was added in the form of urea at three different stages of plant growth, applying a total of 0, 50 or 100 pounds of nitrogen. The yield of lint from DP 90 was increased where nitrogen was added; however, there were no differences in yield with the timing nor total amount of nitrogen added. In the case of S-6, fertilizer nitrogen did not increase yield.
Doerge, Thomas A., T. C. Tucker e T. W. McCreary. "Effect of Nitrification Inhibitors on Nitrogen Utilization Efficiency in Sweet Corn". College of Agriculture, University of Arizona (Tucson, AZ), 1991. http://hdl.handle.net/10150/221451.
Testo completoDarwiche, Amal Omar 1964. "Effect of cytokinin, gibberellin, and nitrogen applications on the growth of eldarica pine seedlings". Thesis, The University of Arizona, 1989. http://hdl.handle.net/10150/276979.
Testo completoCampbell, Richard John. "Effect of nitrogen fertilization on the physiological damage and subsequent recovery of mite-infested apple leaves /". This resource online, 1988. http://scholar.lib.vt.edu/theses/available/etd-04122010-083649/.
Testo completoBeltran, Casellas Gemma. "Effect of low temperature fermentation and nitrogen content on wine yeast metabolism". Doctoral thesis, Universitat Rovira i Virgili, 2005. http://hdl.handle.net/10803/8651.
Testo completoPer intentar millorar el procés fermentatiu i la qualitat del producte final es van fixar els següents objectius:
- L'estudi del metabolisme del llevat a baixes temperatures de fermentació (13ºC), i la seva influència en aspectes com la cinètica fermentativa, el creixement dels llevats; el metabolisme lipídic; la producció dels aromes, i la expressió gènica global del llevat.
- El coneixement del metabolisme nitrogenat del llevat en la fermentació alcohòlica, així com en les addicions de nitrogen realitzades a diferents moments de la fermentació.
Els resultats obtinguts mostren que les baixes temperatures, a part d'augmentar la durada de fermentació, augmenten la viabilitat dels llevats al llarg del procés, provoquen canvis en la composició lipídica, augmentant la fluïdesa de la membrana, i milloren la composició aromàtica del vi, incrementant la producció d'aromes beneficiosos i disminuint-ne la de compostos perjudicials pel la qualitat vi final.
Amb l'objectiu d'identificar els mecanismes moleculars que causen aquests canvis metabòlics a baixes temperatures, vam utilitzar la tècnica de "chips de DNA" o "microarrays" per comparar l'expressió global dels gens del llevat fermentant a 13ºC i fermentant a 25ºC. En general, aquest anàlisis de l'expressió global del llevat dut a terme per primer cop en condicions industrials rebel·la importants canvis en la expressió d'alguns gens tant al llarg de la fermentació com entre les dos temperatures. La fermentació a 13ºC presenta l'avantatge d'induir una ràpida resposta al estrès que podria aportar més resistència al llevat al llarg de la fermentació, i per això augmentar-ne la seva viabilitat.
En l'estudi del metabolisme nitrogenat del llevat al llarg de la fermentació, vam observar que en la fermentació alcohòlica les cèl·lules evolucionen d'una situació de repressió per nitrogen al començament de la fermentació, quan hi ha compostos nitrogenats en el medi, a una situació de de-repressió quan el nitrogen ha estat consumit pel llevat. Aquestes situacions de repressió/de-repressió determinen el perfil de consum de l'amoni i dels aminoàcids, els quals determinen a la vegada la producció d'alguns compostos aromàtics. La repressió dels gens de les permeases GAP1 i MEP2, la baixa activitat arginasa o la inhibició en la captació de l'arginina, poden ser considerats bons marcadors de Repressió Catabòlica per Nitrogen (NCR).
L'addició de nitrogen és una pràctica habitual en bodega per evitar problemes fermentatius. Els nostres estudis demostren que el moment de dur a terme aquesta addició condiciona no només la cinètica fermentativa i el creixement del llevat, sinó també el perfil de consum d'amoni i aminoàcids, i la producció de compostos secundaris.
L'assimilació de nitrogen per part dels llevats també depèn de la temperatura de fermentació, la qual determina tant la qualitat com la quantitat dels requeriments nitrogenats dels llevats. A baixa temperatura de fermentació, l'amoni i la glutamina són menys consumits, mentre que els aminoàcids regulats per NCR ho són més.
Aquesta tesi és una aproximació global al comportament del llevat a baixes temperatures i al metabolisme nitrogenat, i ens obra moltes possibilitats d'estudi, punts on s'hauria d'aprofundir per un millor coneixement i millora d'aquestes fermentacions.
Wines produced at low temperatures (10-15ºC) are known to develop certain characteristics of taste and aroma, not only related to primary aroma retention. However, low temperature fermentations have also some disadvantages that comprise an increase of the duration of the process and a higher risk of stuck and sluggish fermentation.
In order to improve the fermentation performance and the quality of wine, we established the following objectives:
- The study of wine yeast metabolism at low temperature fermentation (13ºC), and its influence in aspects as the fermentation kinetic, the yeast growth, the yeast lipid metabolism, the production of aromatic compounds, and the global yeast gene expression.
- The study of nitrogen metabolism of yeast in alcoholic fermentation, as well as the study of nitrogen supplementations at different points of the fermentation.
Our results showed that low temperatures increased the length of fermentation, the yeast viability along the process, but also modified the lipid composition of yeast cells, increasing the membrane fluidity, and improved the aromatic composition of the wine, increasing the flavour-active compounds and decreasing the unpleasant ones such as acetic acid and fusel alcohols.
To identify the molecular mechanism that causes these changes in aroma profiles and to verify that 13°C-fermentation does not hinder other cellular properties, we compared the expression programs during wine fermentation at 13ºC and 25°C (using Microarrays technology), and tentatively correlated the differential genes expression with changes in intracellular lipid content, and in the production of flavour-active metabolites
This genome-wide analysis carried out for the first time with a commercial yeast strain under true industrial conditions revealed many major differential genes expression both during the course of the wine fermentation and between two fermentation temperatures. With respect to industrial output, wine fermentation conducted at 13°C presents the advantage to induce an early cold stress response that apparently does not penalize the wine fermentation process, further than the longest fermentation length.
In the study of the nitrogen metabolism of yeast along the fermentation we observed that in wine fermentations the cells evolve from a nitrogen-repressed situation at the beginning of the process to a nitrogen-derepressed situation as the nitrogen is consumed. These nitrogen-repressed/derepressed conditions determined the different patterns of ammonium and amino acid consumption. Arginine and alanine were hardly used under the repressed conditions, while the uptake of branched-chain and aromatic amino acids increased. The repression of GAP1 and MEP2 genes in the cells, low arginase activity or inhibition of arginine uptake could be considered as a good Nitrogen Catabolite Repression markers.
Winemakers systematically supplement grape musts with diammonium phosphate to prevent nitrogen-related fermentation problems. The timing of the nitrogen additions influenced the biomass yield, the fermentation performance, the patterns of ammonium and amino acid consumption, and the production of secondary metabolites. These nitrogen additions induced a nitrogen-repressed situation in the cells, and this situation determined which nitrogen sources were selected.
Nitrogen assimilation also depends on fermentation temperature. Fermentation temperature is an important factor determining utilization of nitrogen sources during fermentation of grape juice, and influences the quantity and the quality of nitrogen requirement. Ammonium and glutamine, the preferred source for biomass production, are less consumed at low temperature. Likewise amino acids that are only taken up under derepressed conditions (arginine, alanine, asparagine, etc.) are more consumed at low temperature.
The information provided by this thesis represents a starting point for deciphering the regulatory circuits during wine fermentation, overall at low temperature, and should help us to understand the properties of wine yeasts. Our results open up a lot of interesting perspectives that will further our knowledge of wine yeast metabolism during wine fermentations.
Lotfollahi, Mohammad. "The effect of subsoil mineral nitrogen on grain protein concentration of wheat". Title page, table of contents and summary only, 1996. http://web4.library.adelaide.edu.au/theses/09PH/09phl882.pdf.
Testo completoRoy, Vincent. "Effect of exhaust gas recirculation on fuel consumption and nitrogen oxides emissions". Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2001. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp05/MQ63554.pdf.
Testo completoStrange, Malinda Range. "The effect of pathology on the stable isotopes of carbon & nitrogen". Diss., Online access via UMI:, 2006.
Cerca il testo completoVenkataraman, Prakash. "Effect of nitrogen filling on tire rolling resistance and vehicle fuel economy". Connect to this title online, 2007. http://etd.lib.clemson.edu/documents/1202498590/.
Testo completoSmith, Jennifer M. "The effect of nitrogen on competitive interactions in a weed-crop community". Thesis, University of East Anglia, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.357959.
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