Tesi sul tema "East Indians"
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Lin, Yan. ""Cricket is in the blood" (Re)producing Indianness: Families negotiating diasporic identity through cricket in Singapore". Thesis, University of Canterbury. Sociology and Anthropology, 2006. http://hdl.handle.net/10092/996.
Testo completoKulanjiyil, Thomaskutty I. "Culture and psychology understanding Indian culture and its implications for counseling Asian Indian immigrants in the United States /". Online full text .pdf document, available to Fuller patrons only, 2003. http://www.tren.com.
Testo completoHunjan, Sandeep. "East Indians in Canada, changing conceptions of love". Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp01/MQ30946.pdf.
Testo completoMatthew, Mulamootil Ronnie Bolls Paul David. "Model ethnicity and product class involvement white Americans' attitude toward advertisements featuring Asian-Indian models /". Diss., Columbia, Mo. : University of Missouri--Columbia, 2007. http://hdl.handle.net/10355/4958.
Testo completoGreenan, Michele Anne. "Three early-middle Woodland mortuary sites in East Central Indiana : a study in paleopathology". Virtual Press, 1999. http://liblink.bsu.edu/uhtbin/catkey/1137663.
Testo completoDepartment of Anthropology
Edwin, Shanthi S. "Effective ways to evangelize Asian Indians in the United States". Theological Research Exchange Network (TREN) Access this title online, 2005. http://www.tren.com.
Testo completoPoynting, Robert Jeremy. "Literature and cultural pluralism : East Indians in the Caribbean". Thesis, University of Leeds, 1985. http://etheses.whiterose.ac.uk/821/.
Testo completoBush, Ratimaya Sinha. "Festivals, rituals and ethnicity among East Indians in Trinidad /". The Ohio State University, 1997. http://rave.ohiolink.edu/etdc/view?acc_num=osu1487944660929446.
Testo completoOcita, James. "Diasporic imaginaries : memory and negotiation of belonging in East African and South African Indian narratives". Thesis, Stellenbosch : Stellenbosch University, 2013. http://hdl.handle.net/10019.1/80354.
Testo completoENGLISH ABSTRACT: This dissertation explores selected Indian narratives that emerge in South Africa and East Africa between 1960 and 2010, focusing on representations of migrations from the late 19th century, with the entrenchment of mercantile capitalism, to the early 21st century entry of immigrants into the metropolises of Europe, the US and Canada as part of the post-1960s upsurge in global migrations. The (post-)colonial and imperial sites that these narratives straddle re-echo Vijay Mishra‘s reading of Indian diasporic narratives as two autonomous archives designated by the terms, "old" and "new" diasporas. The study underscores the role of memory both in quests for legitimation and in making sense of Indian marginality in diasporic sites across the continent and in the global north, drawing together South Asia, Africa and the global north as continuous fields of analysis. Categorising the narratives from the two locations in their order of emergence, I explore how Ansuyah R. Singh‘s Behold the Earth Mourns (1960) and Bahadur Tejani‘s Day After Tomorrow (1971), as the first novels in English to be published by a South African and an East African writer of Indian descent, respectively, grapple with questions of citizenship and legitimation. I categorise subsequent narratives from South Africa into those that emerge during apartheid, namely, Ahmed Essop‘s The Hajji and Other Stories (1978), Agnes Sam‘s Jesus is Indian and Other Stories (1989) and K. Goonam‘s Coolie Doctor: An Autobiography by Dr Goonam (1991); and in the post-apartheid period, including here Imraan Coovadia‘s The Wedding (2001) and Aziz Hassim‘s The Lotus People (2002) and Ronnie Govender‘s Song of the Atman (2006). I explore how narratives under the former category represent tensions between apartheid state – that aimed to reveal and entrench internal divisions within its borders as part of its technology of rule – and the resultant anti-apartheid nationalism that coheres around a unifying ―black‖ identity, drawing attention to how the texts complicate both apartheid and anti-apartheid strategies by simultaneously suggesting and bridging differences or divisions. Post-apartheid narratives, in contrast to the homogenisation of "blackness", celebrate ethnic self-assertion, foregrounding cultural authentication in response to the post-apartheid "rainbow-nation" project. Similarly, I explore subsequent East African narratives under two categories. In the first category I include Peter Nazareth‘s In a Brown Mantle (1972) and M.G. Vassanji‘s The Gunny Sack (1989) as two novels that imagine Asians‘ colonial experience and their entry into the post-independence dispensation, focusing on how this transition complicates notions of home and national belonging. In the second category, I explore Jameela Siddiqi‘s The Feast of the Nine Virgins (1995), Yasmin Alibhai-Brown‘s No Place Like Home (1996) and Shailja Patel‘s Migritude (2010) as post-1990 narratives that grapple with political backlashes that engender migrations and relocations of Asian subjects from East Africa to imperial metropolises. As part of the recognition of the totalising and oppressive capacities of culture, the three authors, writing from both within and without Indianness, invite the diaspora to take stock of its role in the fermentation of political backlashes against its presence in East Africa.
AFRIKAANSE OPSOMMING: Hierdie studie fokus op geselekteerde narratiewe deur skrywers van Indiër-oorsprong wat tussen 1960 en 2010 in Suid-Afrika en Oos-Afrika ontstaan om uitbeeldings van migrerings en verskuiwings vanaf die einde van die 19e eeu, ná die vestiging van handelskapitalisme, immigrasie in die vroeë 21e eeu na die groot stede van Europa, die VS en Kanada, te ondersoek, met die oog op navorsing na die toename in globale migrasies. Die (post-)koloniale en imperial liggings wat in hierdie narratiewe oorvleuel, beam Vijay Mishra se lesing van diasporiese Indiese narratiewe as twee outonome argiewe wat deur die terme "ou" en "nuwe" diasporas aangedui word. Hierdie proefskrif bestudeer die manier waarop herinneringe benut word, nie alleen in die soeke na legitimisering en burgerskap nie, maar ook om tot 'n beter begrip te kom van die omstandighede wat Asiërs na die imperiale wêreldstede loods. Ek kategoriseer die twee narratiewe volgens die twee lokale en in die volgorde waarin hulle verskyn het en bestudeer Ansuyah R Singh se Behold the Earth Mourns (1960) en Bahadur Tejani se Day After Tomorrow (1971) as die eerste roman wat deur 'n Suid-Afrikaanse en 'n Oos-Afrikaanse skrywe van Indiese herkoms in Engels gepubliseer is, en die wyse waarop hulle onderskeidelik die kwessies van burgerskap en legitimisasie benader. In daaropvolgende verhale van Suid-Afrika, onderskei ek tussen narratiewe at hul onstaan in die apartheidsjare gehad het, naamlik The Hajji and Other Stories deur Ahmed Essop, Jesus is Indian and Other Stories (1989) deur Agnes Sam en Coolie Doctor: An Autobiography by Dr. Goonam deur K. Goonam; uit die post-apartheid era kom The Wedding (2001) deur Imraan Covadia en The Lotus People (2002) deur Aziz Hassim, asook Song of the Atman (2006) deur Ronnie Govender. Ek kyk hoe die verhale in die eerste kategorie spanning beskryf tussen die apartheidstaat — en die gevolglike anti-apartheidnasionalisme in 'n eenheidskeppende "swart" identiteit — om die aandag te vestig op die wyse waarop die tekste sowel apartheid- as anti-apartheid strategieë kompliseer deur tegelykertyd versoeningsmoontlikhede en verdeelheid uit te beeld. Post-apartheid verhale, daarenteen, loof eerder etniese selfbemagtiging met die klem op kulturele outentisiteit in reaksie op die post-apartheid bevordering van 'n "reënboognasie", as om 'n homogene "swartheid" voor te staan. Op dieselfde manier bestudeer ek die daaropvolgende Oos-Afrikaanse verhale onder twee kategorieë. In die eerste kategorie sluit ek In an Brown Mantle (1972) deur Peter Nazareth en The Gunny Sack (1989) deur M.G. Vassanjiin, as twee romans wat Asiërs se koloniale geskiedenis en hul toetrede tot die post-onafhanklikheid bedeling uitbeeld (verbeeld) (imagine), met die klem op die wyse waarop hierdie oorgang begrippe van samehorigheid kompliseer. In die tweede kategorie kyk ek na The Feast of the Nine Virgins (1995) deur Jameela Siddiqi, No Place Like Home (1996) deur Yasmin Alibhai en Migritude (2010) deur Shaila Patel as voorbeelde van post-1990 verhale wat probleme met die politieke teenreaksies en verskuiwings van Asiër-onderdane vanuit Oos-Afrika na wêreldstede aanspreek. As deel van die erkenning van die totaliserende en onderdrukkende kapasiteit van kultuur, vra die drie skrywers – as Indiërs en as wêreldburgers – die diaspora om sy rol in die opstook van politieke teenreaksie teen sy teenwoordigheid in Oos-Afrika onder oënskou te neem.
Sick, Rebecca Faye. "Nonmetric trait analysis of four East Central Indiana skeletal populations". Virtual Press, 2000. http://liblink.bsu.edu/uhtbin/catkey/1164848.
Testo completoDepartment of Anthropology
Siri, Raktida Kennon Lisa R. "Indian tourists' motivation, perception, and satisfaction of Bangkok, Thailand". [Denton, Tex.] : University of North Texas, 2009. http://digital.library.unt.edu/permalink/meta-dc-9827.
Testo completoNayar, Shoba. "The theory of navigating cultural spaces". Click here to access this resource online, 2009. http://hdl.handle.net/10292/733.
Testo completoMiller, Kevin Christopher. "A community of sentiment Indo-Fijian music and identity discourse in Fiji and its diaspora /". Diss., Restricted to subscribing institutions, 2008. http://proquest.umi.com/pqdweb?did=1582037101&sid=1&Fmt=2&clientId=1564&RQT=309&VName=PQD.
Testo completoBrar, Navdeep K. "Acculturation and mate selection preferences among Asian-Indians in the United States". Virtual Press, 1998. http://liblink.bsu.edu/uhtbin/catkey/1074529.
Testo completoDepartment of Psychological Science
Kunvar, Yogita. "Reconceptualising notions of South African Indianess : a personal narrative". Thesis, Rhodes University, 2015. http://hdl.handle.net/10962/d1017767.
Testo completoDawood, Zohra Bibi. "Making a community : Indians in Cape Town, circa 1900-1980s". Master's thesis, University of Cape Town, 1993. http://hdl.handle.net/11427/14957.
Testo completoThe contention underlying this dissertation is that ethnic identity and notions of community in South Africa are the result of several intertwined processes, which include historic interventions by the state to create 'groups' and 'nations' as building blocks for apartheid structures. These processes also encompass initiatives by those oppressed to constitute 'oppositional' communities. Both sets of activities have occurred in specific historical and material circumstances. By focusing specifically on a 'group' descended largely from merchant forebears, this study of Cape Indians examines the significance over time of the class, caste and religious cleavages within a constructed 'community'. Moreover, this dissertation discusses the effects of political currents on Indians in the Western Cape whose relatively more privileged position in relation to Indians in Natal and the Transvaal has been instrumental in isolating them from most of the events in the other provinces. It is hoped that this dissertation will contribute not only to the history of the Western Cape but, in a broader sense, also to the history of Indians in South Africa.
Gill, Kuldip. "Health strategies of Indo-Fijian women in the context of Fiji". Thesis, University of British Columbia, 1988. http://hdl.handle.net/2429/28790.
Testo completoArts, Faculty of
Anthropology, Department of
Graduate
Altanero, de la Santísima Metáfora Ti5mothy John Tarek. "Power indexation in language choice in a South African Indian community /". Digital version accessible at:, 1999. http://wwwlib.umi.com/cr/utexas/main.
Testo completoMcRaith, Michael J. "The University of Wisconsin's mental health services and perceptions of Asian-Indian students". Online version, 2009. http://www.uwstout.edu/lib/thesis/2009/2009mcraithm.pdf.
Testo completoVoigt-Graf, Carmen 1970. "The construction of transnational spaces : travelling between India, Fiji and Australia / Carmen Voigt-Graf". Thesis, The University of Sydney, 2002. https://hdl.handle.net/2123/27931.
Testo completoCarlisle, Jeffrey D. "Spanish Relations with the Apache Nations East of the Río Grande". Thesis, University of North Texas, 2001. https://digital.library.unt.edu/ark:/67531/metadc2816/.
Testo completoSwan, Daniel C. "West moon - east moon : an ethnohistory of the peyote religion among the Osage Indians, 1898-1930 /". Full-text version available from OU Domain via ProQuest Digital Dissertations, 1990.
Cerca il testo completoMadhavan, Susan M. "The job satisfaction level of Chinese- and Indian-born engineering faculty at a research I university". Morgantown, W. Va. : [West Virginia University Libraries], 2001. http://etd.wvu.edu/templates/showETD.cfm?recnum=1913.
Testo completoTitle from document title page. Document formatted into pages; contains viii, 225 p. : ill. (some col.). Vita. Includes abstract. Includes bibliographical references (p. 213-216).
Pereira, Peter. "Building bridges with Christ's love to the Asian Indian Hindu community in Chicago". Online full text .pdf document, available to Fuller patrons only, 2002. http://www.tren.com.
Testo completoDullabh, Neela. "An examination of the factors influencing the spatial distribution of the Indian communities in Grahamstown, King William's town, Queenstown and Uitenhage from 1880 to 1991". Thesis, Rhodes University, 1995. http://hdl.handle.net/10962/d1005506.
Testo completoSwiatek, William J. "The residential patterns of immigrants in Greater Philadelphia a comparative study of the Indians, Koreans and Vietnamese, 1980-2000 /". Access to citation, abstract and download form provided by ProQuest Information and Learning Company; downloadable PDF file, 232 p, 2008. http://proquest.umi.com/pqdweb?did=1459905431&sid=3&Fmt=2&clientId=8331&RQT=309&VName=PQD.
Testo completoElixhauser, Sophie. "Nammeq : personal autonomy and everyday communication in the Ammassalik Region, East Greenland". Thesis, University of Aberdeen, 2011. http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=166166.
Testo completoKaladeen, Maria del Pilar. "With eyes of wonder : colonial writing on indentured East Indians in British Guiana 1838-1917". Thesis, Royal Holloway, University of London, 2013. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.591076.
Testo completoEverett, Arthur R. "Developing a model for reaching Native Americans through other tribal peoples the effect of a short-term ministry trip by a tribal team from East Malaysia on the acceptance of outsiders by Pueblo Native Americans in New Mexico /". Online full text .pdf document, available to Fuller patrons only, 2000. http://www.tren.com.
Testo completoSapre, Manasi. "Memories of Motherland: Gender, Diaspora and National Identity in 1990s Indian Popular Culture". Thesis, University of North Texas, 2002. https://digital.library.unt.edu/ark:/67531/metadc3076/.
Testo completoSidhu, Kamaljit Kaur. "Acculturative stress, self esteem and ethnic identity among 2nd generation Sikh adolescents". Thesis, University of British Columbia, 1990. http://hdl.handle.net/2429/31520.
Testo completoEducation, Faculty of
Educational and Counselling Psychology, and Special Education (ECPS), Department of
Graduate
Varghese, Suja. "Nutrition related knowledge, attitudes, practices and needs of Indian immigrants and family members in Newfoundland /". Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape9/PQDD_0018/MQ54913.pdf.
Testo completoCharlton-Stevens, Uther E. "Decolonising Anglo-Indians : strategies for a mixed-race community in late colonial India during the first half of the 20th century". Thesis, University of Oxford, 2012. http://ora.ox.ac.uk/objects/uuid:254b43ad-a0d6-4416-b451-c1ebff58ecce.
Testo completoTruncer, James. "Steatite vessel manufacture in Eastern North America /". Oxford, England : Archaeopress, 2004. http://books.google.com/books?id=NSVmAAAAMAAJ.
Testo completoSumadraji, Sambomurthie. "An assessment of the role of narrative preaching in selected Indian churches in South Africa". Theological Research Exchange Network (TREN), 2002. http://www.tren.com.
Testo completoWagle, Jaya. "Homeland/Split". Thesis, University of North Texas, 2018. https://digital.library.unt.edu/ark:/67531/metadc1404588/.
Testo completoEsat, Fazila. "The social construction of "sexual knowledge" : exploring the narratives of southern African youth of Indian descent in the context of HIV/AIDS". Thesis, Rhodes University, 2003. http://eprints.ru.ac.za/226/.
Testo completoRerceretnam, Marc. "Black Europeans, the Indian coolies and empire : colonialisation and christianized Indians in colonial Malaya & Singapore, c. 1870s - c. 1950s". Phd thesis, Faculty of Economics and Business, 2002. http://hdl.handle.net/2123/7626.
Testo completoNeill, Dawn B. "The effect of urbanization on parental investment decisions among Indo-Fijians /". Thesis, Connect to this title online; UW restricted, 2007. http://hdl.handle.net/1773/6449.
Testo completoHo, Man Yee Portia. "Some aspects of the phonological features of English spoken by school-age Indians in Hong Kong". HKBU Institutional Repository, 1995. http://repository.hkbu.edu.hk/etd_ra/89.
Testo completoJhappan, Carol R. (Carol Radha). "The language of empowerment : symbolic politics and Indian political discourse in Canada". Thesis, University of British Columbia, 1990. http://hdl.handle.net/2429/30656.
Testo completoArts, Faculty of
Political Science, Department of
Graduate
Vadakkan, Mary F. "SURVIVAL: CULTURE-SPECIFIC RESOURCES FOR ASIAN INDIAN ELDERS IN THE COMMUNITY". Connect to this document online, 2005. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=miami1115836553.
Testo completoTitle from first page of PDF document. Document formatted into pages; contains [1], iii, 43 p. Includes bibliographical references (p. 36-37).
Schulein, Stefanie. "The relationship between social capital and income generation amongst Indians in South Africa : an exploratory and comparative study in post-Apartheid South Africa". Thesis, Stellenbosch : Stellenbosch University, 2005. http://hdl.handle.net/10019.1/50515.
Testo completoENGLISH ABSTRACT: Despite the abolition of Apartheid in 1994, the entrenched effects of discriminatory policies remain prevalent in terms of socio-economic inequalities between racial groups in South Africa. Nevertheless, throughout the Apartheid era the Indian population of South Africa seems to have maintained a distinct economic advantage when compared to Africans and Coloureds. This dynamic is indeed puzzling as these three racial groups were all subject to discriminatory Apartheid legislation. In an attempt to find an appropriate explanatory variable for this trend, I tum to the notion of social capital (social networks and the norms of reciprocity and trustworthiness that arise from them). Arriving in South Africa in 1860 as indentured labourers on Natal's sugar plantations, the Indian community in South Africa indeed continues to display distinct dynamics with regards to social organization in the post- Apartheid era. In light of these dynamics, this study aims to assess the relationship between levels of social capital and income generation amongst the African, Coloured and Indian communities in South Africa. It is hypothesized that a distinct set of associational networks within the Indian community, shaped by a specific historical trajectory, are directly related to the heightened income generation capacity of this racial group. The vanous dimensions of social capital assessed in this study include: membership of voluntary organizations, informal social ties, participation in religious organizations and trust. Findings indicate that it is particularly within the realm of informal social ties that Indians derive a distinct economic advantage. The effect remains once the impact of education is taken into consideration. This exploratory study therefore makes a valuable contribution towards the analysis of social capital within South Africa's different race groups, allowing for more valid indicators to be developed in the future. Future studies will need to identify the seeds which need to be planted if social capital is to grow organically, not only within, but more importantly between race groups. This will no doubt make a lasting contribution towards addressing the widespread socio-economic challenges currently faced by South Africa's emerging democracy.
AFRIKAANSE OPSOMMING: Ten spyte van die afskaffing van Apartheid in 1994, is die gevolge van diskriminerende wetgewing in terme van sosio-ekonomiese ongelykhede tussen bevolkingsgroepe in Suid Afrika nog steeds sigbaar. Nietemin het die Indiër bevolking van Suid Afrika, in vergelyking met Kleurlinge en Swartes, gedurende Apartheid 'n duidelike ekonomiese voorsprong behou. Hierdie dinamika is inderdaad verwarrend aangesien al drie hierdie bevolkingsgroepe aan diskriminerende Apartheidswetgewing onderworpe was. In 'n poging om 'n toepaslike verklarende veranderlike vir hierdie tendens te vind, ondersoek hierdie studie sosiale kapitaal (sosiale netwerke en norme van wederkerigheid en vertroue). Die Indiër bevolking, wat in 1860 as kontrakarbeiders op Natal se suikerplantasies in die land aangekom het, toon inderdaad selfs na die afskaffing van Apartheid nog spesifieke tendense met betrekkeng tot hulle onderlinge sosiale bande. Teen die agtergrond van hierdie dinamika het hierdie studie ten doel om die verband tussen vlakke van sosiale kapitaal en inkomstegenerering onder Swartes, Kleurlinge en Indiërs te ontleed. Die hipotese word gestel dat 'n duidelike stel gemeenskaplike netwerke onder Indiërs, gevorm deur spesifieke historiese gebeure, direk verband hou met hierdie bevolkingsgroep se verhoogde kapasiteit vir inkomstegenerering. Die verskillende dimensies van sosiale kapitaal wat in hierdie studie ontleed word, sluit in: lidmaatskap van vrywillige organisasies, informele sosiale bande, deelname aan Godsdienstige aktiwiteite en vertoue. Die studie bevind dat veral informele sosiale bande aan Indiërs 'n duidelike ekonomiese voorsprong bied. Hierdie bevinding bly onveranderd selfs nadat die invloed van opvoeding in ag geneem word. Hierdie verkennende studie lewer dus 'n waardevolle bydrae tot die ontleding van sosiale kapitaal tussen verskillende bevolkingsgroepe in Suid Afrika en baan sodoende die weg vir die ontwikkeling van meer geldige aanwysers in die toekoms. Sulke studies sal die saad moet identifiseer wat geplant moet word om die organiese groei van sosiale kapitaal te stimuleer, nie net binne nie, maar meer belangrik tussen bevolkingsgroepe. Dit sal sonder twyfel 'n blywende bydrae lewer om die sosio-ekonomiese uitdagings wat Suid Afrika se ontwikkelende demokrasie tans ondervind, volledig aan te spreek.
Boshoff, Priscilla. "Diasporic consciousness and Bollywood : South African Indian youth and the meanings they make of Indian film". Thesis, Rhodes University, 2006. http://hdl.handle.net/10962/d1006249.
Testo completoSiri, Raktida. "Indian tourists' motivation, perception, and satisfaction of Bangkok,Thailand". Thesis, University of North Texas, 2009. https://digital.library.unt.edu/ark:/67531/metadc9827/.
Testo completoCourt, Erin. "How transnational actors change inter-state power asymmetries : the role of the Indian diaspora in Indo-Canadian relations on migration". Thesis, University of Oxford, 2011. https://ora.ox.ac.uk/objects/uuid:8501d594-e5c1-47e0-9a08-24b7645f29f2.
Testo completoHughes, Delain. "Complementary Dualities: The Significance of East/West Architectural Difference in Paquimé". Thesis, University of North Texas, 2005. https://digital.library.unt.edu/ark:/67531/metadc4871/.
Testo completoRich, Lisa D. "Feminism in developing countries : the question of the South African Indian". Virtual Press, 1996. http://liblink.bsu.edu/uhtbin/catkey/1014822.
Testo completoDepartment of Sociology
Singh, Karmjit. "Post-positivist study exploring the resettlement experience of professional Asian Indian women". CSUSB ScholarWorks, 1997. https://scholarworks.lib.csusb.edu/etd-project/1329.
Testo completoHallendorff, Michelle-Angelique. "Ironing out haemochromatosis : a study of an Indian family". Thesis, Stellenbosch : Stellenbosch University, 2008. http://hdl.handle.net/10019.1/21458.
Testo completoENGLISH ABSTRACT: Iron metabolism disorders comprise the most common disorders in humans. Hereditary haemochromatosis (HH) is a common condition resulting from inappropriate iron absorption. The most common form of the disease (Type 1) is associated with mutations in the HFE gene. The C282Y homozygous genotype accounts for approximately 80% of all reported cases of HH within the Caucasian population. A second HFE mutation, H63D, is associated with less severe disease expression. The C282Y mutation is extremely rare in Asian and African populations. The H63D mutation is more prevalent and has been observed in almost all populations. Iron overload resulting from haemochromatosis is predicted to be rare in Asian Indian populations and is not associated with common HFE mutations that are responsible for HH in the Caucasian population. The aberrant genes associated with HH in India have not yet been identified. The present study attempted to identify variants in six iron regulatory genes that were resulting in the Type 1 HH phenotype observed in two Asian Indian probands from a highly consanguineous family. The promoter and coding regions of the HMOX1, HFE, HAMP, SLC40A1, CYBRD1 and HJV genes were subjected to mutation analysis. Gene fragments were amplified employing the polymerase chain reaction (PCR) and subsequently subjected to heteroduplex single-strand conformational polymorphism (HEX-SSCP) analysis. Samples displaying aberrations were then analysed using bi-directional semi-automated DNA sequencing analysis to identify any known or novel variants within the six genes. Variants disrupting restriction enzyme recognition sites were genotyped employing restriction fragment length polymorphism (RFLP) analysis. Mutation analysis of the six genes revealed 24 previously identified variants, five novel variants (HFE: 5’UTR-840T→G, CYBRD1: 5’UTR-1813C→T, 5’UTR-1452T→C, 5’UTR- 1272T→C; HJV: 5’UTR-534G→T, 5’UTR-530G→T), one previously described microsatellite and two novel repeats. Variants identified within the SLC40A1, CYBRD1 and HJV genes do not seem to be associated with the iron overload phenotype. A previously described HAMP variant (5’UTR-335G→T) was observed in the homozygous state in both probands. This variant seems to be the genetic aberration responsible for iron overload in this Indian family. The severe juvenile haemochromatosis phenotype usually associated with HAMP mutations, was not exhibited by the two Indian probands. Their symptoms resembled those observed in classic Type 1 HH. It is suggested that variants identified in the HMOX1 and HFE genes are modifying the effect of the HAMP variant and resulting in the less severe disease phenotype. Although this variant has only been identified in one Indian family, it could shed some light in the hunt for the iron-loading gene in India.
AFRIKAANSE OPSOMMING: Oorerflike hemochromatose (OH) is ‘n algemene siektetoestand wat ontstaan as gevolg van oneffektiewe opname van yster in die liggaam. Die mees algemene vorm van die siekte (Tipe 1) word geassosieer met mutasies in die HFE-geen. Die C282Y homosigotiese genotipe is verantwoordelik vir ongeveer 80% van alle gerapporteerde gevalle van OH binne die Kaukasiese bevolking. ‘n Tweede HFE mutasie, H63D, word geassosieer met minder ernstige siekte simptome. Die C282Y mutasie is besonder skaars in Asiese en Afrika bevolkings. Daar word bespiegel dat oorerflike ysteroorlading as gevolg van hemochromatose skaars is in Asiese Indiër bevolkings en word nie geassosieer met algemene HFE mutasies wat verantwoordelik is vir OH in Kaukasiese bevolkings nie. Die abnormale gene wat wél geassosieer word met OH in Indië is tot dusver nog nie identifiseer nie. Die doel van hierdie studie was om die variante in ses yster-regulerende gene te identifiseer wat die Tipe 1 OH fenotipe in hierdie familie veroorsaak. Hierdie fenotipe is waargeneem in twee Asies Indiese familielede afkomstig van ‘n bloedverwante familie. Die promotor en koderingsareas van die HMOX1, HFE, HAMP, SLC40A1, CYBRD1 en HJV gene is gesif vir mutasies. Geen fragmente is geamplifiseer met behulp van die polimerase kettingsreaksie (PKR) en daarna aan heterodupleks enkelstring konformasie polimorfisme (HEX-SSCP) analise blootgestel. PKR produkte wat variasies getoon het, is daarna geanaliseer deur tweerigting semi-geoutomatiseerde DNS volgorde-bepalingsanalise om enige bekende of nuwe variante binne die ses gene te identifiseer. Variante waar restriksie ensiem herkenningsetels teenwoordig is, is verder analiseer met behulp van die restriksie fragment lengte polimorfisme (RFLP) analise sisteem. Mutasie analise van die ses gene het 24 bekende variante, vyf nuwe variante (HFE: 5’UTR- 840T→G, CYBRD1: 5’UTR-1813C→T, 5’UTR-1452T→C, 5’UTR-1272T→C, HJV: 5’UTR-534G→T, 5’UTR-530G→T), een bekende herhaling en twee nuwe herhalings gewys. Variante wat binne die SLC4041, CYBRD1 en HJV gene geïdentifiseer is, blyk nie om by te dra tot die ysteroorladings-fenotipe nie. Die bekende HAMP variant (5’UTR-335G→T) is waargeneem in die homosigotiese toestand in beide van die aangetaste individue. Hierdie variant blyk om die genetiese fout te wees wat verantwoordelik is vir die ysteroorlading in die betrokke Indiese familie. Die erge juvenielehemochromatose fenotipe wat meestal geassosieer word met HAMP-mutasies, is nie waargeneem in hierdie familie nie. Hul simptome kom ooreen met die simptome van die klassieke Tipe 1 OH. Dit blyk moontlik te wees dat die variante identifiseer in die HMOX1 en HFE gene die impak van die HAMP variant modifiseer en die matiger siekte-fenotipe tot gevolg het. Alhoewel hierdie variant slegs in een Indiese familie geïdentifiseer is, kan dit lig werp op die soektog na die veroorsakende ysterladingsgeen in Indië.