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Articoli di riviste sul tema "Dual lifetime referencing"

Autore: Grafiati
Pubblicato: 22 febbraio 2025

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1

Huber, Christian, Ingo Klimant, Christian Krause e Otto S. Wolfbeis. "Dual Lifetime Referencing as Applied to a Chloride Optical Sensor". Analytical Chemistry 73, n. 9 (maggio 2001): 2097–103. http://dx.doi.org/10.1021/ac9914364.

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2

Chen, Wan-Har, Evelyn Armstrong, Peter W. Dillingham, Stephen C. Moratti, Courtney Ennis e Christina M. McGraw. "Dual-Lifetime Referencing (t-DLR) Optical Fiber Fluorescent pH Sensor for Microenvironments". Sensors 23, n. 21 (31 ottobre 2023): 8865. http://dx.doi.org/10.3390/s23218865.

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Abstract (sommario):
The pH behavior in the μm to cm thick diffusion boundary layer (DBL) surrounding many aquatic species is dependent on light-controlled metabolic activities. This DBL microenvironment exhibits different pH behavior to bulk seawater, which can reduce the exposure of calcifying species to ocean acidification conditions. A low-cost time-domain dual-lifetime referencing (t-DLR) interrogation system and an optical fiber fluorescent pH sensor were developed for pH measurements in the DBL interface. The pH sensor utilized dual-layer sol-gel coatings of pH-sensitive iminocoumarin and pH-insensitive Ru(dpp)3-PAN. The sensor has a dynamic range of 7.41 (±0.20) to 9.42 ± 0.23 pH units (95% CI, T = 20 °C, S = 35), a response time (t90) of 29 to 100 s, and minimal salinity dependency. The pH sensor has a precision of approximately 0.02 pHT units, which meets the Global Ocean Acidification Observing Network (GOA-ON) “weather” measurement quality guideline. The suitability of the t-DLR optical fiber pH sensor was demonstrated through real-time measurements in the DBL of green seaweed Ulva sp. This research highlights the practicability of optical fiber pH sensors by demonstrating real-time pH measurements of metabolic-induced pH changes.
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3

Liebsch, Gregor, Ingo Klimant, Christian Krause e Otto S. Wolfbeis. "Fluorescent Imaging of pH with Optical Sensors Using Time Domain Dual Lifetime Referencing". Analytical Chemistry 73, n. 17 (settembre 2001): 4354–63. http://dx.doi.org/10.1021/ac0100852.

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4

Borisov, Sergey M., Gerhard Neurauter, Claudia Schroeder, Ingo Klimant e Otto S. Wolfbeis. "Modified Dual Lifetime Referencing Method for Simultaneous Optical Determination and Sensing of Two Analytes". Applied Spectroscopy 60, n. 10 (ottobre 2006): 1167–73. http://dx.doi.org/10.1366/000370206778664590.

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5

Begemann, Jens, e Antje C. Spiess. "Dual lifetime referencing enables pH-control for oxidoreductions in hydrogel-stabilized biphasic reaction systems". Biotechnology Journal 10, n. 11 (settembre 2015): 1822–29. http://dx.doi.org/10.1002/biot.201500198.

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6

Maierhofer, Maximilian, Sergey M. Borisov e Torsten Mayr. "Optical Ammonia Sensor for Continuous Bioprocess Monitoring". Proceedings 2, n. 13 (19 novembre 2018): 1041. http://dx.doi.org/10.3390/proceedings2131041.

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Abstract (sommario):
We present an optical ammonia sensor suitable for bioprocess monitoring. A fluorescent dye is physically entrapped in a polyurethane hydrogel forming an emulsion system with vinylterminated polydimethylsiloxane (PDMS). The sensing layer is covered by a hydrophobic porous membrane which excludes hydrophilic substances. Ammonia, diffuses through this barrier and PDMS to the protonated dye, whereby it deprotonates the dye and switches off its emission. Readout is performed with a miniaturized phase fluorimeter combined with optical fibers. Dual-lifetime referencing (DLR) acts as detection method and Egyptian blue as reference material.
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7

Lin, Xuyan, Wenting Qiu, Gianmarco Domenico Suarez e Stefan Nagl. "Extracellular pH Monitoring of Live Single Cells in Microdroplets Using Dual-Labelled Fluorinated Silica Nanoparticles and Time-Domain Dual Lifetime Referencing". Chemosensors 10, n. 10 (21 settembre 2022): 379. http://dx.doi.org/10.3390/chemosensors10100379.

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Fluorinated silica nanoparticles doped with Ruthenium-tris-1,10-phenanthroline dichloride on the inside and covalently conjugated with perfluorooctyltriethoxysilane and fluorescein isothiocyanate on the outside were developed and served several functions; the fluorination of the particles served to stabilize droplets in a microfluidic system at their interface to the continuous phase for single-cell experiments, and the two dyes provided for intrinsically referenced pH readout according to the time-domain dual lifetime referencing scheme. Apart from eliminating the droplet-to-droplet transport, these nanoparticles at the interface of the droplets generated rigid substrates that were suitable for the proliferation of adherent cells in the droplets without additional matrices. Cancer and non-cancer cell lines with culture media were allowed to proliferate in the droplets and the extracellular pH was monitored. These nanoparticles used in a microdroplet system could measure the pH of the extracellular microenvironment of single cells and provide support for the growth of cells in droplets of around 50 µm diameter. The pHe showed 6.84 ± 0.04 and 6.81 ± 0.04 for cancer cells (MCF-7 and A549, respectively) and 7.36 ± 0.03 for healthy cells (HUVEC), after a 10-h incubation, which can be potentially applied in distinguishing tumor from non-tumor cells. Capable of assisting cell culture and pH sensing in droplet microfluidic systems, the dye-conjugated fluorinated nanoparticles described in this work offer possibilities in a variety of biochemical or environmental analytical applications.
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8

Rowe, H. M., Sing Po Chan, J. N. Demas e B. A. DeGraff. "Self-Referencing Intensity Measurements Based on Square-Wave Gated Phase-Modulation Fluorimetry". Applied Spectroscopy 57, n. 5 (maggio 2003): 532–37. http://dx.doi.org/10.1366/000370203321666551.

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Abstract (sommario):
An adaptation of square-wave gated phase-modulation (GPM) fluorimetry allows for self-referenced intensity measurements without the complexity of dual excitation or dual emission wavelengths. This AC technique utilizes square-wave excitation, gated detection, a reference emitter, and a sensor molecule. The theory and experimental data demonstrating the effectiveness and advantages of the adapted GPM scheme are presented. One component must have an extremely short lifetime relative to the other. Both components are affected identically by changes in intensity of the excitation source, but the sensor intensity also depends on the concentration of the analyte. The fluctuations of the excitation source and any optical transmission changes are eliminated by ratioing the sensor emission to the reference emission. As the concentration of the analyte changes, the corresponding sensor intensity changes can be quantified through several schemes including digitization of the signal and digital integration or AC methods. To measure pH, digital methods are used with Na3[Tb(dpa)3] (dpa = 2,6-pyridinedicarboxylic acid) as the long-lived reference molecule and fluorescein as the short-lived sensor molecule. Measurements from the adapted GPM scheme are directly compared to conventional ratiometric measurements. Good agreement between the data collection methods is demonstrated through the apparent pKa. For the adapted GPM measurements, conventional measurements, and a global fit the apparent pKa values agree within less than 2%. A key element of the adapted GPM method is its insensitivity to fluctuations in the source intensity. For a roughly 8-fold change in the excitation intensity, the signal ratio changes by less than 3%.
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9

Boniello, Caterina, Torsten Mayr, Juan M. Bolivar e Bernd Nidetzky. "Dual-lifetime referencing (DLR): a powerful method for on-line measurement of internal pH in carrier-bound immobilized biocatalysts". BMC Biotechnology 12, n. 1 (2012): 11. http://dx.doi.org/10.1186/1472-6750-12-11.

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10

Poehler, Elisabeth, Christin Herzog, Madeleine Suendermann, Simon A. Pfeiffer e Stefan Nagl. "Development of microscopic time-domain dual lifetime referencing luminescence detection for pH monitoring in microfluidic free-flow isoelectric focusing". Engineering in Life Sciences 15, n. 3 (5 marzo 2015): 276–85. http://dx.doi.org/10.1002/elsc.201400081.

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11

Maierhofer, Maximilian, Veronika Rieger e Torsten Mayr. "Optical ammonia sensors based on fluorescent aza-BODIPY dyes— a flexible toolbox". Analytical and Bioanalytical Chemistry 412, n. 27 (24 agosto 2020): 7559–67. http://dx.doi.org/10.1007/s00216-020-02891-3.

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Abstract (sommario):
Abstract We present three types of optical ammonia sensors suitable for environmental, bioprocess, and reaction monitoring. A respective fluorescent BF2-chelated tetraarylazadipyrromethene dye (aza-BODIPYs) is physically entrapped in a polyurethane hydrogel (HydroMed D4) forming an emulsion system with vinyl-terminated polydimethylsiloxane (PDMS). The analyte-sensitive layer is covered by a hydrophobic membrane which excludes hydrophilic substances. Three different protection layers are tested, whereby the Teflon and the hydrophobic PES layers outperform a PDMS/TiO2 layer. Response times within their dynamic range of 15 s can be achieved, whereas the PDMS/TiO2-covered sensor requires at least 390 s. The three sensors entail the following concentration areas: first sensor 3 μg L−1–3 mg L−1 (LOD 0.23 μg L−1), second sensor 0.1–30 mg L−1 (LOD 28 μg L−1), and third sensor 3 mg L−1–1 g L−1 (LOD 0.51 mg L−1). Readout is performed with a commercially available phase fluorimeter combined with optical fibers. Dual-lifetime referencing (DLR) is used as referencing method and Egyptian blue acts as an inert reference material. No cross-sensitivity to pH changes can be detected.
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12

Ding, Longjiang, Ying Lian, Zhenzhen Lin, Zeyu Zhang e Xu-dong Wang. "Long-Term Quantitatively Imaging Intracellular Chloride Concentration Using a Core-/Shell-Structured Nanosensor and Time-Domain Dual-Lifetime Referencing Method". ACS Sensors 5, n. 12 (30 novembre 2020): 3971–78. http://dx.doi.org/10.1021/acssensors.0c01671.

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13

Grengg, Cyrill, Bernhard Mueller, Florian Mittermayr, Torsten Mayr, Sergey Borisov e Martin Dietzel. "Optical pH imaging of concrete exposed to chemically corrosive environments". MATEC Web of Conferences 199 (2018): 02007. http://dx.doi.org/10.1051/matecconf/201819902007.

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Major types of chemical concrete degradation such as carbonation, leaching and acid attacks are strongly associated with decreasing internal or external pH. Thus a precise determination of the latter is crucial for the assessment regarding the degree of corrosion and corresponding development of prevention strategies. Conventional pH measure methods for concrete, such as phenolphthalein indicator, pore solution extractions and flat surface electrodes have proven to contain significant limitations and inadequacies. This contribution presents the application of sensor foils based on luminescent pH sensitive dyes entrapped in a polymeric hydrogel matrix, to quantify and image the distribution of surface pH of concrete based construction materials. An imaging technique called time-domain dual lifetime referencing (t-DLR) was used, which suppresses artifacts from scattering of the background and other light inhomogeneities. Using this methodology high-resolution pH profiles of concrete samples exposed to carbonation and biogenic acid corrosion were recorded.
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14

Tufan, Tuna B., e Ulkuhan Guler. "A Transcutaneous Carbon Dioxide Monitor Based on Time-Domain Dual Lifetime Referencing". IEEE Transactions on Biomedical Circuits and Systems, 2023, 1–12. http://dx.doi.org/10.1109/tbcas.2023.3277398.

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15

Clarke, Jennifer S., Matthew P. Humphreys, Eithne Tynan, Vassilis Kitidis, Ian Brown, Matthew Mowlem e Eric P. Achterberg. "Characterization of a Time-Domain Dual Lifetime Referencing pCO2 Optode and Deployment as a High-Resolution Underway Sensor across the High Latitude North Atlantic Ocean". Frontiers in Marine Science 4 (15 dicembre 2017). http://dx.doi.org/10.3389/fmars.2017.00396.

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