Articoli di riviste: "DNA microarrays"

1

Weitzman, Jonathan B. "DNA/DNA microarrays". Genome Biology 2 (2001): spotlight—20010813–03. http://dx.doi.org/10.1186/gb-spotlight-20010813-03.

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Hofman, Paul. "DNA Microarrays". Nephron Physiology 99, n. 3 (24 febbraio 2005): p85—p89. http://dx.doi.org/10.1159/000083764.

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Cook, Stuart A., e Anthony Rosenzweig. "DNA Microarrays". Circulation Research 91, n. 7 (4 ottobre 2002): 559–64. http://dx.doi.org/10.1161/01.res.0000036019.55901.62.

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Biesen, R., e T. Häupl. "DNA-Microarrays". Zeitschrift für Rheumatologie 70, n. 9 (30 settembre 2011): 803–8. http://dx.doi.org/10.1007/s00393-011-0869-4.

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Niemeyer, Christof M., e Dietmar Blohm. "DNA Microarrays". Angewandte Chemie International Edition 38, n. 19 (4 ottobre 1999): 2865–69. http://dx.doi.org/10.1002/(sici)1521-3773(19991004)38:19<2865::aid-anie2865>3.0.co;2-f.

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Aparna, G. M., e Kishore K. R. Tetala. "Recent Progress in Development and Application of DNA, Protein, Peptide, Glycan, Antibody, and Aptamer Microarrays". Biomolecules 13, n. 4 (27 marzo 2023): 602. http://dx.doi.org/10.3390/biom13040602.

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Abstract (sommario):

Microarrays are one of the trailblazing technologies of the last two decades and have displayed their importance in all the associated fields of biology. They are widely explored to screen, identify, and gain insights on the characteristics traits of biomolecules (individually or in complex solutions). A wide variety of biomolecule-based microarrays (DNA microarrays, protein microarrays, glycan microarrays, antibody microarrays, peptide microarrays, and aptamer microarrays) are either commercially available or fabricated in-house by researchers to explore diverse substrates, surface coating, immobilization techniques, and detection strategies. The aim of this review is to explore the development of biomolecule-based microarray applications since 2018 onwards. Here, we have covered a different array of printing strategies, substrate surface modification, biomolecule immobilization strategies, detection techniques, and biomolecule-based microarray applications. The period of 2018–2022 focused on using biomolecule-based microarrays for the identification of biomarkers, detection of viruses, differentiation of multiple pathogens, etc. A few potential future applications of microarrays could be for personalized medicine, vaccine candidate screening, toxin screening, pathogen identification, and posttranslational modifications.

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Whipple, Mark Eliot, e Winston Patrick Kuo. "DNA Microarrays in Otolaryngology-Head and Neck Surgery". Otolaryngology–Head and Neck Surgery 127, n. 3 (settembre 2002): 196–204. http://dx.doi.org/10.1067/mhn.2002.127383.

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OBJECTIVES: Our goal was to review the technologies underlying DNA microarrays and to explore their use in otolaryngology-head and neck surgery. STUDY DESIGN: The current literature relating to microarray technology and methodology is reviewed, specifically the use of DNA microarrays to characterize gene expression. Bioinformatics involves computational and statistical methods to extract, organize, and analyze the huge amounts of data produced by microarray experiments. The means by which these techniques are being applied to otolaryngology-head and neck surgery are outlined. RESULTS: Microarray technologies are having a substantial impact on biomedical research, including many areas relevant to otolaryngology-head and neck surgery. CONCLUSIONS: DNA microarrays allow for the simultaneous investigationof thousands of individual genes in a single experiment. In the coming years, the application of these technologies to clinical medicine should allow for unprecedented methods ofdiagnosis and treatment. SIGNIFICANCE: These highly parallel experimental techniques promise to revolutionize gene discovery, disease characterization, and drug development.

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Paredes, Carlos J., Ryan S. Senger, Iwona S. Spath, Jacob R. Borden, Ryan Sillers e Eleftherios T. Papoutsakis. "A General Framework for Designing and Validating Oligomer-Based DNA Microarrays and Its Application to Clostridium acetobutylicum". Applied and Environmental Microbiology 73, n. 14 (25 maggio 2007): 4631–38. http://dx.doi.org/10.1128/aem.00144-07.

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ABSTRACT While DNA microarray analysis is widely accepted as an essential tool for modern biology, its use still eludes many researchers for several reasons, especially when microarrays are not commercially available. In that case, the design, construction, and use of microarrays for a sequenced organism constitute substantial, time-consuming, and expensive tasks. Recently, it has become possible to construct custom microarrays using industrial manufacturing processes, which offer several advantages, including speed of manufacturing, quality control, no up-front setup costs, and need-based microarray ordering. Here, we describe a strategy for designing and validating DNA microarrays manufactured using a commercial process. The 22K microarrays for the solvent producer Clostridium acetobutylicum ATCC 824 are based on in situ-synthesized 60-mers employing the Agilent technology. The strategy involves designing a large library of possible oligomer probes for each target (i.e., gene or DNA sequence) and experimentally testing and selecting the best probes for each target. The degenerate C. acetobutylicum strain M5 lacking the pSOL1 megaplasmid (with 178 annotated open reading frames [genes]) was used to estimate the level of probe cross-hybridization in the new microarrays and to establish the minimum intensity for a gene to be considered expressed. Results obtained using this microarray design were consistent with previously reported results from spotted cDNA-based microarrays. The proposed strategy is applicable to any sequenced organism.

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Fesseha, Haben, e Hiwot Tilahun. "Principles and Applications of Deoxyribonucleic Acid Microarray: A Review". Pathology and Laboratory Medicine – Open Journal 3, n. 1 (30 marzo 2021): 1–9. http://dx.doi.org/10.17140/plmoj-3-109.

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Deoxyribonucleic acid (DNA) microarrays are collections of DNA probes arranged on a base pair and the latest commercialized molecular diagnostic technologies that offer high throughput results, more sensitive and require less time. It is the most reliable and widely accepted tool facilitating the simultaneous identification of thousands of genetic elements even a single gene. Microarrays are powerful new tools for the investigation of global changes in gene expression profiles in cells and tissues. The different types of DNA microarray or DNA chip devices and systems are described along with their methods of fabrication and their use. The DNA microarrays assembly process is automatized and further miniaturized. DNA microarrays are used in the search of various specific genes or in gene polymorphism and expression analysis. They will be widely used to investigate the expression of various genes connected with various diseases in order to find the causes of these diseases and to enable their accurate treatment. Generally, microarray analysis is not only applied for gene expression studies, but also used in immunology, genotyping, diagnostics and sequence analysis. Additionally, microarray technology being developed and applied to new areas of proteomics, cancer research, and cellular analysis.

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Peiffer, Daniel, Ken Cho e Yongchol Shin. "Xenopus DNA Microarrays". Current Genomics 4, n. 8 (1 novembre 2003): 665–72. http://dx.doi.org/10.2174/1389202033490097.

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Schofield, W. C. E., J. McGettrick, T. J. Bradley, J. P. S. Badyal e S. Przyborski. "Rewritable DNA Microarrays". Journal of the American Chemical Society 128, n. 7 (febbraio 2006): 2280–85. http://dx.doi.org/10.1021/ja056367r.

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HENRY, CELIA M. "STANDARDIZING DNA MICROARRAYS". Chemical & Engineering News 82, n. 31 (2 agosto 2004): 36–39. http://dx.doi.org/10.1021/cen-v082n031.p036.

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13

Jack, Philippa, e David Boyle. "DNA microarrays for pathogen detection and characterisation". Microbiology Australia 27, n. 2 (2006): 68. http://dx.doi.org/10.1071/ma06068.

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DNA microarrays have three main potential diagnostic uses in clinical microbiology: detection of known pathogens, pathogen typing and novel pathogen discovery. Although DNA microarray platforms offer the ability to screen for a large number of agents in parallel, sensitivity is dependent on the ability to obtain adequate amounts of pathogen nucleic acids from collected samples. In general, high levels of sensitivity require a PCR amplification step using specific primer sets, subsequently reducing the overall scope of the microarray assay. At present, relatively high costs, restricted sample throughput capabilities and validation difficulties are also major factors limiting the implementation of DNA microarray assays in diagnostic microbiology laboratories.

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Chagovetz, Alexander, e Steve Blair. "Real-time DNA microarrays: reality check". Biochemical Society Transactions 37, n. 2 (20 marzo 2009): 471–75. http://dx.doi.org/10.1042/bst0370471.

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DNA microarrays are plagued with inconsistent quantifications and false-positive results. Using established mechanisms of surface reactions, we argue that these problems are inherent to the current technology. In particular, the problem of multiplex non-equilibrium reactions cannot be resolved within the framework of the existing paradigm. We discuss the advantages and limitations of changing the paradigm to real-time data acquisition similar to real-time PCR methodology. Our analysis suggests that the fundamental problem of multiplex reactions is not resolved by the real-time approach itself. However, by introducing new detection chemistries and analysis approaches, it is possible to extract target-specific quantitative information from real-time microarray data. The possible scope of applications for real-time microarrays is discussed.

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Chiodi, Elisa, Allison M. Marn, Matthew T. Geib e M. Selim Ünlü. "The Role of Surface Chemistry in the Efficacy of Protein and DNA Microarrays for Label-Free Detection: An Overview". Polymers 13, n. 7 (26 marzo 2021): 1026. http://dx.doi.org/10.3390/polym13071026.

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The importance of microarrays in diagnostics and medicine has drastically increased in the last few years. Nevertheless, the efficiency of a microarray-based assay intrinsically depends on the density and functionality of the biorecognition elements immobilized onto each sensor spot. Recently, researchers have put effort into developing new functionalization strategies and technologies which provide efficient immobilization and stability of any sort of molecule. Here, we present an overview of the most widely used methods of surface functionalization of microarray substrates, as well as the most recent advances in the field, and compare their performance in terms of optimal immobilization of the bioreceptor molecules. We focus on label-free microarrays and, in particular, we aim to describe the impact of surface chemistry on two types of microarray-based sensors: microarrays for single particle imaging and for label-free measurements of binding kinetics. Both protein and DNA microarrays are taken into consideration, and the effect of different polymeric coatings on the molecules’ functionalities is critically analyzed.

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Call, Douglas R., Marlene K. Bakko, Melissa J. Krug e Marilyn C. Roberts. "Identifying Antimicrobial Resistance Genes with DNA Microarrays". Antimicrobial Agents and Chemotherapy 47, n. 10 (ottobre 2003): 3290–95. http://dx.doi.org/10.1128/aac.47.10.3290-3295.2003.

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ABSTRACT We developed and tested a glass-based microarray suitable for detecting multiple tetracycline (tet) resistance genes. Microarray probes for 17 tet genes, the β-lactamase bla TEM-1 gene, and a 16S ribosomal DNA gene (Escherichia coli) were generated from known controls by PCR. The resulting products (ca. 550 bp) were applied as spots onto epoxy-silane-derivatized, Teflon-masked slides by using a robotic spotter. DNA was extracted from test strains, biotinylated, hybridized overnight to individual microarrays at 65°C, and detected with Tyramide Signal Amplification, Alexa Fluor 546, and a microarray scanner. Using a detection threshold of 3× the standard deviation, we correctly identified tet genes carried by 39 test strains. Nine additional strains were not known to harbor any genes represented on the microarray, and these strains were negative for all 17 tet probes as expected. We verified that R741a, which was originally thought to carry a novel tet gene, tet(I), actually harbored a tet(G) gene. Microarray technology has the potential for screening a large number of different antibiotic resistance genes by the relatively low-cost methods outlined in this paper.

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Stoevesandt, O., M. He e M. J. Taussig. "Repeatable printing of protein microarrays from DNA microarrays". New Biotechnology 25 (settembre 2009): S360. http://dx.doi.org/10.1016/j.nbt.2009.06.961.

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18

Kostrzynska, M., e A. Bachand. "Application of DNA microarray technology for detection, identification, and characterization of food-borne pathogens". Canadian Journal of Microbiology 52, n. 1 (1 gennaio 2006): 1–8. http://dx.doi.org/10.1139/w05-105.

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DNA microarrays represent the latest advance in molecular technology. In combination with bioinformatics, they provide unparalleled opportunities for simultaneous detection of thousands of genes or target DNA sequences and offer tremendous potential for studying food-borne microorganisms. This review provides an up-to-date look at the application of DNA microarray technology to detect food-borne pathogenic bacteria, viruses, and parasites. In addition, it covers the advantages of using microarray technology to further characterize microorganisms by providing information for specific identification of isolates, to understand the pathogenesis based on the presence of virulence genes, and to indicate how new pathogenic strains evolved epidemiologically and phylogenetically.Key words: DNA microarrays, food-borne pathogens, detection.

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Herrera, Henry J., e Marlon Gancino. "DNA microarrays: Recent Advances". Bionatura 2, n. 3 (15 agosto 2017): 404–6. http://dx.doi.org/10.21931/rb/2017.02.03.13.

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Dai, Wei, Mona A. Sheikh, Olgica Milenkovic e Richard G. Baraniuk. "Compressive Sensing DNA Microarrays". EURASIP Journal on Bioinformatics and Systems Biology 2009 (2009): 1–12. http://dx.doi.org/10.1155/2009/162824.

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Meloni, R. "DNA microarrays and pharmacogenomics". Pharmacological Research 49, n. 4 (aprile 2004): 303–8. http://dx.doi.org/10.1016/j.phrs.2003.06.001.

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Sassolas, Audrey, Béatrice D. Leca-Bouvier e Loïc J. Blum. "DNA Biosensors and Microarrays". Chemical Reviews 108, n. 1 (gennaio 2008): 109–39. http://dx.doi.org/10.1021/cr0684467.

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Rathod, Pradipsinh K., Karthikeyan Ganesan, Rhian E. Hayward, Zbynek Bozdech e Joseph L. DeRisi. "DNA microarrays for malaria". Trends in Parasitology 18, n. 1 (gennaio 2002): 39–45. http://dx.doi.org/10.1016/s1471-4922(01)02153-5.

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Marcotte, Eric R., Lalit K. Srivastava e Rémi Quirion. "DNA microarrays in neuropsychopharmacology". Trends in Pharmacological Sciences 22, n. 8 (agosto 2001): 426–36. http://dx.doi.org/10.1016/s0165-6147(00)01741-7.

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Smith, L. "DNA microarrays and development". Human Molecular Genetics 12, n. 90001 (2 aprile 2003): 1R—8. http://dx.doi.org/10.1093/hmg/ddg053.

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Trost, Brett, Catherine A. Moir, Zoe E. Gillespie, Anthony Kusalik, Jennifer A. Mitchell e Christopher H. Eskiw. "Concordance between RNA-sequencing data and DNA microarray data in transcriptome analysis of proliferative and quiescent fibroblasts". Royal Society Open Science 2, n. 9 (settembre 2015): 150402. http://dx.doi.org/10.1098/rsos.150402.

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DNA microarrays and RNA sequencing (RNA-seq) are major technologies for performing high-throughput analysis of transcript abundance. Recently, concerns have been raised regarding the concordance of data derived from the two techniques. Using cDNA libraries derived from normal human foreskin fibroblasts, we measured changes in transcript abundance as cells transitioned from proliferative growth to quiescence using both DNA microarrays and RNA-seq. The internal reproducibility of the RNA-seq data was greater than that of the microarray data. Correlations between the RNA-seq data and the individual microarrays were low, but correlations between the RNA-seq values and the geometric mean of the microarray values were moderate. The two technologies had good agreement when considering probes with the largest (both positive and negative) fold change (FC) values. An independent technique, quantitative reverse-transcription PCR (qRT-PCR), was used to measure the FC of 76 genes between proliferative and quiescent samples, and a higher correlation was observed between the qRT-PCR data and the RNA-seq data than between the qRT-PCR data and the microarray data.

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I. A., Zaloilo. "APPLYING OF DNA- MICROARRAYS IN A MODERN FISH-FARMING". Biotechnologia Acta 8, n. 4 (agosto 2015): 9–20. http://dx.doi.org/10.15407/biotech8.04.009.

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Lacroix, M., N. Zammatteo, J. Remacle e G. Leclercq. "A Low-Density DNA Microarray for Analysis of Markers in Breast Cancer". International Journal of Biological Markers 17, n. 1 (gennaio 2002): 5–23. http://dx.doi.org/10.1177/172460080201700102.

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Breast cancer remains a major cause of death in women from Western countries. In the near future, advances in both nucleic acids technology and tumor biology should be widely exploited to improve the diagnosis, prognosis, and outcome prediction of this disease. The DNA microarray, also called biochip, is a promising tool for performing massive, simultaneous, fast, and standardized analyses of multiple molecular markers in tumor samples. However, most currently available microarrays are expensive, which is mainly due to the amount (several thousands) of different DNA capture sequences that they carry. While these high-density microarrays are best suited for basic studies, their introduction into the clinical routine remains hypothetical. We describe here the principles of a low-density microarray, carrying only a few hundreds of capture sequences specific to markers whose importance in breast cancer is generally recognized or suggested by the current medical literature. We provide a list of about 250 of these markers. We also examine some potential difficulties (homologies between marker and/or variant sequences, size of sequences, etc.) associated with the production of such a low-cost microarray.

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&NA;. "DNA microarrays in clinical practice". Inpharma Weekly &NA;, n. 1306 (settembre 2001): 3. http://dx.doi.org/10.2165/00128413-200113060-00004.

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Akin, H. E., D. A. O. Karabay, J. R. Kyle, A. P. Mills, C. S. Ozkan e M. Ozkan. "Electronic Microarrays in DNA Computing". Journal of Nanoscience and Nanotechnology 11, n. 3 (1 marzo 2011): 1859–65. http://dx.doi.org/10.1166/jnn.2011.3422.

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Akin, H. E., D. A. O. Karabay, J. R. Kyle, A. P. Mills, Jr., C. Ozkan e M. Ozkan. "Electronic Microarrays in DNA Computing". Journal of Nanoscience and Nanotechnology 11, n. 6 (1 giugno 2011): 4717–23. http://dx.doi.org/10.1166/jnn.2011.38844717.

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Booth, David E. "Cancer Diagnostics With DNA Microarrays". Technometrics 49, n. 4 (novembre 2007): 492–93. http://dx.doi.org/10.1198/tech.2007.s686.

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Chow, B. Y., C. J. Emig e J. M. Jacobson. "Photoelectrochemical synthesis of DNA microarrays". Proceedings of the National Academy of Sciences 106, n. 36 (21 agosto 2009): 15219–24. http://dx.doi.org/10.1073/pnas.0813011106.

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Russell, S. "DNA Microarrays: Gene Expression Applications". Heredity 89, n. 5 (28 ottobre 2002): 402. http://dx.doi.org/10.1038/sj.hdy.6800150.

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BURLEIGH, B. A. "ProbingTrypanosoma cruzibiology with DNA microarrays". Parasitology 128, S1 (ottobre 2004): S3—S10. http://dx.doi.org/10.1017/s0031182004006559.

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The application of genome-scale approaches to studyTrypanosoma cruzi–host interactions at different stages of the infective process is becoming possible with sequencing and assembly of theT. cruzigenome nearing completion and sequence information available for both human and mouse genomes. Investigators have recently begun to exploit DNA microarray technology to analyze host transcriptional responses toT. cruziinfection and dissect developmental processes in the complexT. cruzilife-cycle. Collectively, information generated from these and future studies will provide valuable insights into the molecular requirements for establishment ofT. cruziinfection in the host and highlight the molecular events coinciding with disease progression. While the field is in its infancy, the availability of genomic information and increased accessibility to relatively high-throughput technologies represents a significant advancement toward identification of novel drug targets and vaccine candidates for the treatment and prevention of Chagas' disease.

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Hughes, Timothy R., e Daniel D. Shoemaker. "DNA microarrays for expression profiling". Current Opinion in Chemical Biology 5, n. 1 (febbraio 2001): 21–25. http://dx.doi.org/10.1016/s1367-5931(00)00163-0.

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van Berkum, Nynke L., e Frank CP Holstege. "DNA microarrays: raising the profile". Current Opinion in Biotechnology 12, n. 1 (febbraio 2001): 48–52. http://dx.doi.org/10.1016/s0958-1669(00)00173-7.

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Conzone, Samuel D., e Carlo G. Pantano. "Glass slides to DNA microarrays". Materials Today 7, n. 3 (marzo 2004): 20–26. http://dx.doi.org/10.1016/s1369-7021(04)00122-1.

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Wooster, Richard. "Cancer classification with DNA microarrays". Trends in Genetics 16, n. 8 (agosto 2000): 327–29. http://dx.doi.org/10.1016/s0168-9525(00)02064-3.

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Shoemaker, Daniel D., e Peter S. Linsley. "Recent developments in DNA microarrays". Current Opinion in Microbiology 5, n. 3 (giugno 2002): 334–37. http://dx.doi.org/10.1016/s1369-5274(02)00327-2.

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Reymond, Philippe. "DNA microarrays and plant defence". Plant Physiology and Biochemistry 39, n. 3-4 (marzo 2001): 313–21. http://dx.doi.org/10.1016/s0981-9428(00)01235-3.

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Henry, Celia M. "Focus: DNA microarrays in toxicology." Analytical Chemistry 71, n. 13 (luglio 1999): 462A—464A. http://dx.doi.org/10.1021/ac990494m.

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Yasokawa, Daisuke, e Hitoshi Iwahashi. "Toxicogenomics using yeast DNA microarrays". Journal of Bioscience and Bioengineering 110, n. 5 (novembre 2010): 511–22. http://dx.doi.org/10.1016/j.jbiosc.2010.06.003.

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P, Deepthi, e C. H. Renumadhavi. "DNA Microarrays and Smart Pooling." IOSR Journal of Pharmacy and Biological Sciences 9, n. 1 (2014): 61–64. http://dx.doi.org/10.9790/3008-09136164.

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Kim, C., M. Li, A. Lowe, N. Venkataramaiah, K. Richmond, J. Kaysen e F. Cerrina. "DNA microarrays: An imaging study". Journal of Vacuum Science & Technology B: Microelectronics and Nanometer Structures 21, n. 6 (2003): 2946. http://dx.doi.org/10.1116/1.1627802.

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Ramaswamy, Sridhar, e Todd R. Golub. "DNA Microarrays in Clinical Oncology". Journal of Clinical Oncology 20, n. 7 (1 aprile 2002): 1932–41. http://dx.doi.org/10.1200/jco.2002.20.7.1932.

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ABSTRACT: Aberrant gene expression is critical for tumor initiation and progression. However, we lack a comprehensive understanding of all genes that are aberrantly expressed in human cancer. Recently, DNA microarrays have been used to obtain global views of human cancer gene expression and to identify genetic markers that might be important for diagnosis and therapy. We review clinical applications of these novel tools, discuss some important recent studies, identify promising avenues of research in this emerging field of study, and discuss the likely impact that expression profiling will have on clinical oncology.

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Kochzius, M., M. Nölte, H. Weber, N. Silkenbeumer, S. Hjörleifsdottir, G. O. Hreggvidsson, V. Marteinsson et al. "DNA Microarrays for Identifying Fishes". Marine Biotechnology 10, n. 2 (13 febbraio 2008): 207–17. http://dx.doi.org/10.1007/s10126-007-9068-3.

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Ho, Shuk-Mei, e Kin-Mang Lau. "DNA microarrays in prostate cancer". Current Urology Reports 3, n. 1 (febbraio 2002): 53–60. http://dx.doi.org/10.1007/s11934-002-0011-x.

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Horváth, Szatmár, Zoltán Janka e Károly Mirnics. "Analyzing Schizophrenia by DNA Microarrays". Biological Psychiatry 69, n. 2 (gennaio 2011): 157–62. http://dx.doi.org/10.1016/j.biopsych.2010.07.017.

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Patarakul, K. "DNA microarrays in infectious diseases". Chulalongkorn Medical Journal 52, n. 3 (maggio 2008): 147–53. http://dx.doi.org/10.58837/chula.cmj.52.3.1.

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Articoli di riviste sul tema "DNA microarrays"

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