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Tesi sul tema "DNA fingerprinting of plants"

Autore: Grafiati

Pubblicato: 4 giugno 2021

Ultima modifica: 30 luglio 2024

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1

Ho, Siu-hong. "Isolation and characterization of Panax Ginseng repetitive DNA sequences for DNA fingerprinting /". Hong Kong : University of Hong Kong, 1998. http://sunzi.lib.hku.hk/hkuto/record.jsp?B19737816.

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2

何兆康 e Siu-hong Ho. "Isolation and characterization of Panax Ginseng repetitive DNA sequences for DNA fingerprinting". Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 1998. http://hub.hku.hk/bib/B31215282.

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3

Morin, Geneviève. "Metabolite fingerprinting tools to detect differences between transgenic and conventional crops". Thesis, McGill University, 2007. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=101629.

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A concern in transgenic crops is the potential risk posed by unintended effects which could result from genetic transformation. The objective of this work was to develop an untargeted approach that could characterize transgenic crops, as well as conventional crops, at the molecular level. An experimental approach was designed and used to compare conventional and transgenic soybean varieties. Varieties were compared using their metabolite fingerprints obtained by reverse-phase high performance liquid chromatography (HPLC) and both the analytical and biological variability were assessed. Multivariate and univariate statistical analyses were applied to the data to detect significant differences between the varieties. It was found that transgenic variety PS 46 RR was the most different variety analyzed and that it differed most from Mandarin (Ottawa) and AC Dundas. The statistical analyses also determined that PS 46 RR differed more from the conventional varieties tested than 2601R did.

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4

Cheung, Kin Lok. "Investigation of (3-mercaptopropyl) trimethoxysilane (MPTS)-modified surface and DNA microarray for genotyping of traditional Chinese medicinal plants /". View Abstract or Full-Text, 2003. http://library.ust.hk/cgi/db/thesis.pl?BIOL%202003%20CHEUNGK.

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Thesis (M. Phil.)--Hong Kong University of Science and Technology, 2003.
Includes bibliographical references (leaves 103-111). Also available in electronic version. Access restricted to campus users.

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5

Honing, Jennifer. "Evaluation and implementation of DNA-based diagnostic methodology to distinguish wheat genotypes". Thesis, Link to the online version, 2007. http://hdl.handle.net/10019/638.

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6

Micha, Caterina. "Establishment of phylogenetic relationships within the genus Phragmipedium using RAPD-PCR fingerprinting". Virtual Press, 1995. http://liblink.bsu.edu/uhtbin/catkey/958787.

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DNA fingerprinting was applied for the molecular elucidation of taxonomic relationships within a genus of orchids which have previously been based on morphological characteristics. Phragmipediwn consists of 15-20 species native to Central and South America. This research project included two studies. In the first study DNA was isolated from 11 samples (including two unidentified ones). These individuals, which were mostly hybrids, were found in the Wheeler Orchid Collection and Species Bank at Ball State University. In order to position Phragmipediwn within the orchid family fingerprinting was also performed on individuals in the sister taxa, Cypripedium and Paphiopedium, which are members of the same subfamily, and on a member of the outgroup taxon Vanda. The polymerase chain reaction (PCR) was employed to yield fingerprints resulting from the use of random primers. Fifty nine random amplified polymorphic DNA (RAPD) bands were obtained using 5 different primers to yield 107 polymorphic bands. As many as 75% of genetic loci were found to be shared between hybrids that resulted from a cross of more than one individual in the same section. However the percentage dropped to 35-65 % when only one parent was shared in the cross. Furthermore, the sister group taxa Cypripedium and Paphilopedium shared from 12 % -35 % of their polymorphic loci with the members of the genus Phragmipedium. The outgroup taxon Vanda shared 17% of its polymorphic loci with the rest of the samples.In a second study DNA was isolated from one member of each of the five sections of the genus Phragmipedium, and RAPD-PCR fingerprinting was used to compare their genetic similarities to that of the two sister taxa and the outgroup taxon. It was found that individuals in different genera shared 25% or less of their polymorphic bands. Between sections of the same genus 20-50% of genetic loci were shared. Two sections, Platypetalwn and Phragmipedium showed the highest degree of genetic relatedness (41-53%). Again the outgoup taxon shared less than 20% Phragmipediwn samples on the phenograms produced but the percentage was again insignificant. However, genetic analyses of the members of the section Lorifolia gave conflicting results: 46% genetic identity was observed in the first trial and 20% in the second.In conclusion, RAPD-PCR fingerprinting results appeared to be effective in the positioning of sections within a genus indicating the degree of similarity of closely related taxa. Also RAPD-PCR was able to place an unknown individual within a specific section of the genus. However, it could not be employed to determine the identity of unknown species due to the high degree of genetic diversity observed between even closely related individuals.
Department of Biology

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7

Zhang, Yanbo. "Molecular approach to the authentication of lycium barbarum and its related species". HKBU Institutional Repository, 2000. http://repository.hkbu.edu.hk/etd_ra/227.

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8

Saxon, Herbert. "The molecular biology of orchids : transformation by Agrobacterium Tumefaciens and DNA fingerprinting". Virtual Press, 1995. http://liblink.bsu.edu/uhtbin/catkey/941575.

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The work reported here was done at the Wheeler Orchid Collection and Species Bank and the Department of Biology at Ball State University. We have developed a research teaching program with two applied research goals: genetically transforming and DNA fingerprinting orchid tissue. As part of their molecular biology education, students have investigated the genetic transformation of orchids for mitigating viral symptoms and the identification of unknown orchids by DNA fingerprinting. In a second application of the technology, DNA fingerprinting has been used to determine evolutionary relationships and to quantify genetic diversity among orchids.This dissertation details the background and need for this project and the research that was done to start it. As the early work has, developed and students have added their contributions, the data have developed into two papers formatted for submission to scientific journals. They are included as results.The first is a project designed to insert exognenous DNA into orchid tissue. The soil microbe Agrobacterium tumefaciens causes crown-gall tumors to develop in its plant hosts by inserting DNA into their cells which then controls the biosynthesis of development-controlling hormones. A. tumefaciens which has been disarmed has been routinely used to bioengineer dicotyledonous plants but its use has been rare on monocotyledons. In this paper, we report that A. tumefaciens transformed embryonic orchid tissue and caused alteration in its normal developmental course.The second paper details the DNA fingerprinting of tissue from Aplectrum hymale, a terrestrial orchid native to this climate. Three populations of A. hymale have been sampled and DNA extracted from the tissue samples. RAPD primers were used to prime PCR amplifications of random sequences of the DNA and the amplified DNA was visualized by gel electrophoresis. Loci of the resulting bands were treated as potentially multiallelic gene loci and heterozygosity between and within subpopulations was calculated. We report that the three populations could be partially differentiated by this procedure and that the two populations located nearest to each other yielded the least between -ubpopulation heterozygosity. We report very high levels of genetic diversity between individuals within small subpopulations in spite of the fact that these subpopulations are considered to be primarily clonal in reproductive nature.
Department of Biology

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9

Nogueira, Lia. "Non-tariff barriers and technology trade and welfare implications /". Online access for everyone, 2008. http://www.dissertations.wsu.edu/Dissertations/Summer2008/l_nogueira_072308.pdf.

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10

Rinaldi, Catherine. "Authentication of the Panax genus plants used in Traditional Chinese Medicine (TCM) using Randomly Amplified Polymorphic DNA (RAPD) analysis". University of Western Australia. Centre for Forensic Science, 2007. http://theses.library.uwa.edu.au/adt-WU2008.0054.

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[Truncated abstract] Traditional medicines are used by millions of people throughout the world as their primary source of medical care. A range of materials are in used traditional medicines including plant and animal parts. Even though the traditional medicine trade is estimated to be worth sixty billion dollars annually the trade remains largely unregulated. Unscrupulous practices by vendors to increase their profit margins such as substituting and adulterating expensive material with cheaper varieties go unchecked. This can be dangerous to consumers because some substitutions involve poisonous material. Also, animal parts from endangered species can find their way into traditional medicines, therefore there needs to be a way to identify them in traditional medicines to prosecute poachers. The traditional techniques used for the identification of material used in Traditional Chinese Medicine (TCM) include, morphological, histological, chemical and immunological analysis. However, these techniques have their limitations. This makes applying multiple techniques essential to provide thorough authentication of the material. DNA profiling provides a technique well suited to analysing material used in TCM. DNA profiling is advantageous over other techniques used to authenticate material used in TCM because it requires only a small sample amount, can determine the cultivator, be used on all forms of TCM and potentially distinguish the components of mixtures. ... Therefore, profiles of different species/individual are different and species? can be distinguished. Commercially sold traditional medicines are processed which is likely to degrade the DNA of the sample making extraction and amplification difficult. Here an organic Phenol:Chloroform extraction technique extracted DNA from commercial dried root samples. The extracted DNA was amplifiable using RAPD primers. The RAPD primers used here produced enough polymorphic bands to distinguish different plant species. They were used to distinguish commercial samples that were sold as three different species within the Panax genus, Panax ginseng, Panax quinquefolium and Panax notoginseng and genetically unrelated plant material; Potato and Eleutherococcus senticosus. Liquid samples and mixtures were also profiled with the RAPD primers to determine whether the RAPD primers provide enough distinguishing ability to analyse these forms of TCM. DNA was extracted from the liquid samples, one a ginseng drink and the other an ginseng extractum. However, there was no reliability in the production of PCR products. The analysis of the mixture samples found that not enough polymorphic bands were produced by the RAPD primers used here to identify Panax species within mixtures of two Panax species. While when P. ginseng was mixed with a genetically unrelated sample there was enough polymorphism to differentiate the two samples in the mixture. The results of this research show that RAPD analysis provides a simple and inexpensive technique to begin analysis of materials used in TCM. Using RAPD analysis it is possible to distinguish Panax plant species from each other. However, the RAPD primers used here did not provide enough reproducibility or polymorphism to analyse liquid and mixtures of Panax species plants.

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11

Adeyemi, Samson Adebowale. "Evaluation of the possible application of cowpea genotypes in the farming systems of the Eastern Cape Province, South Africa". Thesis, University of Fort Hare, 2012. http://hdl.handle.net/10353/d1007539.

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Characterization studies on the genetic diversity among cultivated cowpea (Vigna unguiculata (L.) varieties are valuable tools to optimize the use of available genetic resources by farmers, local communities, researchers and breeders. Eight cowpea (Vigna unguiculata (L.) genotypes ( Vegetable cowpea, Ivory grey, Okhalweni, Fahari, Fahari dark, 97K-1069-8, IT93K-73h, and 129-3) were subjected to molecular, morphological and agronomical characterization. DNA amplification fingerprinting markers were used to evaluate the genetic diversity among the eight genotypes. Nine random arbitrary primers were used to screen the eight genotypes to assess their ability to reveal polymorphisms in cowpea, and seven of them were selected for use in characterizing the total sample. A total of 43 bands were generated which are all polymorphic. On the average, the primers generated a total of 6.1 polymorphic bands. The resulting data-matrix included 43 analysed bands with a total of 344 characters. Neighbour joining analysis was used to generate the dendrogram, clustering the genotypes into two groups at an agglomerate coefficient of 0.30 irrespective of their geographical origins. The results also showed the presence of significant differences in morphological and quality traits among the genotypes. Fahari yielded the highest concentration of crude protein (46.51 mg/mg dry leaf) while Vegetable cowpea yielded the lowest (24.41 mg/mg dry leaf). The influence of manure was also found to be effective by increasing the crude protein content of the genotypes as shown by Fahari dark with an average of 53.53 mg/mg dry leaf as opposed to 39.85 mg/mg dry leaf without manure application. Although some small clusters grouped accessions of the same growth habits, a general lack of agreement between clustering and morphological features was observed. It can therefore be concluded that the significant differences between the molecular genetic analysis using DAF-PCR markers, morphologic characters and yield traits can be important tools to identify and discriminates the different cowpea genotypes.

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12

Soleimani, Vahab D. "Genome dynamics in barley (Hordeum vulgare L) cultivars: Molecular diversity, evolution, and DNA fingerprinting". Thesis, University of Ottawa (Canada), 2005. http://hdl.handle.net/10393/29264.

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In the absence of whole genome sequencing, molecular markers are indispensable tools for the study of genome evolution, genetic diversity measurements, and genotype identification. We have used sequence-specific amplified polymorphism (S-SAP) markers that were derived from the BARE-1, an active retrotransposon of barley, to measure the contribution of this element to the evolution of barley genome among 103 cultivars that are commonly grown in Canada and the United States. The results were compared to the genome diversity measures obtained by the single nucleotide polymorphisms (SNP). The barley populations were divided into groups based on various agronomic traits such as end use, i.e., feed versus malting, and seed morphology, i.e., naked versus covered kernel. Analysis of the genetic structure in the population using analysis of molecular variance (AMOVA) for both S-SAP and SNP attributed the largest co-variance component (90%) to the genetic diversity among cultivars within groups. Co-variance component between groups was about 6% which indicated that there was no justification for population differentiation along the set based upon agronomic traits. Genetic relationships among cultivars was assessed by cluster analysis with UPGMA and found to vary substantially between S-SAP and SNP datasets. Quantitative analysis of BARE-1 retrotransposon with real-time PCR in a small group of cultivars showed significant differences in the copy number of the element among cultivars. Most of the BARE-1 elements were in the form of solo LTRs, indicating a high rate of homologous recombination between retrotransposon copies in the genome. Differences of up to 3000 BARE-1 copies per haploid genome were found among cultivars that have been developed and registered within the past three decades. Informative SNPs such as those with high polymorphic information content (PIC) values were used to generate identification keys to distinguish barley cultivars which were otherwise indistinguishable at the morphological and biochemical levels.

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13

Rodrigues, Marjory Xavier. "Molecular characterization of bacterial isolates and microbiome: study of mastitic milk, bulk tank milk, and cheese processing plants". Universidade de São Paulo, 2016. http://www.teses.usp.br/teses/disponiveis/11/11141/tde-30092016-185025/.

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The present study aimed to evaluate bacterial isolates and the microbiome of dairies. The specific aims were: to characterize Staphylococcus spp. isolated from mastitic milk, to evaluate the presence of Lactococcus in mastitic milk as a potential causative agent of mastitis, to evaluate the association between microbiome and milk quality parameters, and to characterize Staphylococcus spp. isolated from production lines of Minas Frescal cheese. The detection of genes encoding virulence factors (enterotoxins (sea, seb, sec, sed, see, seg, seh, sei, selj, selk, sell, selm, seln, selo, selp, seIq, ser, ses, set, selu, selv, and selx), hemolysins (hla, hlb, hld, hlg, and hlgv), exfoliative toxins (eta, etb, and etd), Panton-Valentine leukocidin (pvl), and toxic shock syndrome toxin (tst)), genes encoding antibiotic resistance (resistance to tetracycline (tetK, tetL, and tetM), erythromycin (ermA, ermB, and ermC), methicillin (mecA and mecC), and tobramycin (ant(4\')-Ia)), molecular typing (spa, SCCmec, and agr types), and phenotyping regarding antibiotic resistance were performed in staphylococci isolates from mastitic milk, and from cheese processing plant samples. Staphylococcus aureus was identified in the majority of isolates from both origins. Several virulence factor genes were detected. The distribution of genes encoding staphylococcal enterotoxins (85.0% - 85.7% of isolates were positive for one or more enterotoxin gene) was highlighted and the gene related to H toxin was the most prevalent. Methicillin-resistant Staphylococcus aureus were identified in isolates from mastitic milk (4.1%) and cheese processing (6.0%); the genotyping and phenotyping of these isolates were described. t605 had the highest frequency in the S. aureus population studied. In mastitic milk, Lactococcus was suggested as the causative agent of an outbreak of mastitis in a dairy farm. Using next generation sequencing, the abundance of Lactococcus was observed in microbiome samples. Bacterial isolation and DNA sequencing confirmed the presence of Lactococcus lactis and Lactococcus garvieae. The microbiome of environmental samples and bulk tank milk from the dairy farm showed the Lactococcus genus among the most common bacterial taxa, suggesting other sources of this genus. Regarding milk quality parameters, the microbiome of bulk tank milk from several dairy farms was associated with somatic cell count and bacterial count. The core microbiome was described and many genera of importance were identified. Among the associations performed between microbiome and milk quality parameters, the identification of Streptococcus in samples classified with high somatic cell count and high bacterial count was highlighted. Several bacterial taxa with relative abundance significantly higher in samples classified as high and low cell count and bacterial count were shown. Real-time polymerase chain reaction was also performed associated with bacterial diversity, bacterial taxa, and bacterial count. These findings highlight the need to control and prevent bacterial contamination in the dairy industry, from herd to consumers.
O presente estudo apresentou como objetivo avaliar isolados bacterianos e microbioma de lácteos. Os objetivos específicos foram: caracterizar Staphylococcus spp. isolados de leite de vacas com mastite, avaliar a presença de Lactococcus em leite de vacas com mastite como um potencial agente causador de mastite, avaliar a associação entre microbioma de leite de tanque e parâmetros da qualidade de leite, e caracterizar Staphylococcus spp. isolados de linhas de processamento de queijo Minas frescal. A detecção de genes codificadores de fatores de virulência (enterotoxinas (sea, seb, sec, sed, see, seg, seh, sei, selj, selk, sell, selm, seln, selo, selp, seIq, ser, ses, set, selu, selv, e selx), hemolisinas (hla, hlb, hld, hlg, e hlgv), toxinas exfoliativas (eta, etb e etd), leucocidina de Panton-Valentine (pvl), toxina da síndrome do choque tóxico (tst)), genes codificadores de resistência a antibióticos (resistência a tetraciclina (tetK, tetL e tetM), eritromicina (ermA, ermB e ermC), meticilina (mecA e mecC) e tobramicina (ant(4\')-Ia)), tipagem molecular (spa, SCCmec e agr types), e fenotipagem quanto à resistência a antibióticos foram realizadas em estafilococos isolados de leite de vacas com mastite e de amostras de planta de processamento de queijo. Staphylococcus aureus foi identificado na maioria dos isolados de ambas as origens. Diversos genes de fatores de virulência foram detectados, com destaque para a distribuição de genes codificadores de enterotoxinas estafilocócicas (85,0%-85,7% dos isolados foram positivos para um ou mais genes codificadores de enterotoxinas), sendo o gene relacionado com a toxina H o mais frequente. Staphylococcus aureus meticilina resistente foram identificados em isolados de leite de vacas com mastite (4.1%) e em processamento de queijo (6.0%); o perfil genotípico e fenotípico destes isolados foram descritos. t605 foi o mais freqüente na população de S. aureus estudada. Em leite de vacas com mastite, Lactococcus foi sugerido como o agente causador de um surto de mastite numa fazenda leiteira. Usando sequenciamento de nova geração, a abundância de Lactococcus foi observada no microbioma das amostras. O isolamento e sequenciamento de DNA confirmaram a presença de Lactococcus lactis e Lactococcus garvieae. O microbioma de amostras ambientais e de leite de tanque da fazenda mostrou o gênero Lactococcus entre os mais comuns, sugerindo outras fontes deste gênero. Contemplando parâmetros da qualidade de leite, o microbioma de leite de tanque de várias fazendas leiteiras foi relacionado com contagem de células somáticas e contagem bacteriana. O core microbiome foi descrito e muitos gêneros bacterianos de importância foram identificados. Dentre as análises realizadas associando microbioma com parâmetros da qualidade de leite, foi destacada a identificação de Streptococcus em amostras classificadas com alta contagem de células somáticas e alta contagem bacteriana. Diversos táxons bacterianos com abundância relativa significativamente maior em amostras classificadas com alta e baixa contagem de células somáticas e contagem bacteriana foram mostrados. Reação em cadeia da polimerase em tempo real também foi realizada e associada com diversidade bacteriana, táxons bacterianos e contagem bacteriana. Estes levantamentos confirmam a necessidade de controlar e prevenir a contaminação bacteriana na indústria de lácteos, do rebanho leiteiro até os consumidores.

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Roring, Solvig Mary Margaret. "DNA fingerprinting of Mycobacterium bovis". Thesis, Queen's University Belfast, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.287426.

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15

Zhu, Jiahui. "DNA fingerprinting in Oryza sativa L". Thesis, University of East Anglia, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.338095.

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16

Kennedy, Bobbie-Jo. "DNA fingerprinting of Native American skeletal remains". Virtual Press, 1995. http://liblink.bsu.edu/uhtbin/catkey/958779.

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The purpose of this project was to determine if the human skeletal remains of two distinct Native American cemeteries, found in close geographic proximity, represent the same population. These archaeological sites are similar in location and artifacts. Burial practices, however, vary between the sites. These differences may represent class distinction or a difference in the times the cemeteries were used. Radiocarbon techniques have given dates of AD 230±300 and AD 635±105 for these two sites. Several methods of DNA isolation were compared for their ability to yield PCR amplifiable DNA. DNA isolation using a combination of CTAB and phenol/chloroform/isoamyl alcohol (24:24:1) provided the best results and yielded amplifiable DNA form two individuals, Hn I (8F-410) and Hn 10 ( 27F-8-14 b). Purification of the DNA by extraction from low melting agarose gel was required prior to PCR, and PCR conditions were optimized to maximize the DNA yields. Regions of the mitochondrial DNA (mtDNA) genome of isolated DNA were amplified by PCR using primers which are specific for the HincII region of the mtDNA genome. Inability of restriction enzyme HincII to digest the amplified DNA of these two individuals suggested that they belong to the Native American mtDNA lineage C characterized by the loss of this restriction site.
Department of Anthropology

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17

Meng, Anming. "DNA fingerprinting and minisatellite variation of swans". Thesis, University of Nottingham, 1990. http://eprints.nottingham.ac.uk/13889/.

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Genetic variation in natural populations of four species of swans (Cygnus bewickii, Cygnus olor, Cygnus buccinator and Cygnus cygnus) has been investigated by examining minisatellite loci using human DNA fingerprinting probes pSPT19.6 and pSPT18.15. It has been found that swan minisatellites are highly variable. However, the degree of variation depends on the population structure and species. Bewick's Swans at Slimbridge have the highest degree of minisatellite variation, Whooper Swans at Caerlaverock come second, and then Mute Swans, and Trumpeter Swans in Montana. Comparative study of DNA fingerprints among populations and among species suggested that swan minisatellites are subject to specific as well as population differentiation, although the function of minisatellites remains an unsolved mystery. Hypervariable minisatellites of swans that are detected by DNA fingerprinting are stably inherited as codominant markers. DNA fingerprinting has been used to study mating behaviour of Mute and Whooper Swans in the wild The results showed that the Whooper swans were almost strictly monogamous and Mute Swans exhibited an adaptable reproductive system. A genomic library from Cygnus olor was constructed and dozens of minisatellites were isolated. Most of the cloned swan minisatellites were variable, some showed specific variation, and one (pcoMS6.1) detected RFLPs in PstI digests of Trumpeter Swans.

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Carter, Royston Edwin. "Development adaptations & applications of DNA fingerprinting". Thesis, University of Nottingham, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.336944.

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Derksen, Linda Anne. "Agency and structure in the history of DNA profiling : the stabilization and standardization of a new technology /". Diss., Connect to a 24 p. preview or request complete full text in PDF format. Access restricted to UC IP addresses, 2003. http://wwwlib.umi.com/cr/ucsd/fullcit?p3083460.

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20

Choy, Yan-tsun. "Statistical evaluation of mixed DNA stains". Click to view the E-thesis via HKUTO, 2009. http://sunzi.lib.hku.hk/hkuto/record/B42664287.

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21

Groft, Donald G. "DNA fingerprinting of Alberta bull trout (Salvelinus confluentus) populations". Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp01/MQ38445.pdf.

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Groft, Donald G., e University of Lethbridge Faculty of Arts and Science. "DNA fingerprinting of Alberta bull trout (Salvelinus confluentus) populations". Thesis, Lethbridge, Alta. : University of Lethbridge, Faculty of Arts and Science, 1997, 1997. http://hdl.handle.net/10133/80.

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Bull trout (Salvelinus confluentus) populations from Alberta river drainage systems were compared using molecular techniques. Restriction fragment length polymorphisms (RFLP's) within the NDI and ND5/6 regions of the mitochondrial genome were observed. In addition, randomly amplified polymorphic DNA profiles (RAPD's) from total genomic DNA extracts were compared. Interdrainage comparisons using mtDNA revealed significant population heterogeneity among Alberta bull trout. Percent sequence divergence in mtDNA ranged from 0.14% to 0.92%. Most fish in each population were composed of a small number of common haplotypes, and the remaining fish displayed rare or locally unique haplotypes. RAPD profiles were used to calculate genetic distance values for Alberta, Canada and Montana, U.S.A. populations. Both Nei and Cavalli-Sforza distance values were used to generate neighbor-joining, FITCH and KITSCH distance trees. Two genetically distinct groups of bull trout were revealed by the RAPD analysis and the possiblity that post-glacial bull trout populations are derived from two separate refugia is suggested.
xvii, 161 leaves : ill. ; 28 cm.

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Burr, Mark Daniel 1949. "An evaluation of DNA fingerprinting methods for subtyping Salmonella". Diss., The University of Arizona, 1996. http://hdl.handle.net/10150/290630.

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The use of DNA typing and fingerprinting methods to identify and discriminate strains of bacteria, including Salmonella, has increased dramatically in recent years. Traditional typing methods, including serotyping and phage typing, have often not adequately discriminated strains, nor have they always identified virulent or antibiotic resistant strains. In a literature review, DNA-based methods, including plasmid analysis, restriction fragment length polymorphism (RFLP) analysis, and polymerase chain reaction (PCR) fingerprinting methods were evaluated. Plasmid analysis, including plasmid profiles and plasmid fingerprints have been shown to be useful primarily in short-term investigations of disease outbreak. However, plasmid profiles or possession of individual plasmids have generally not been good indicators of cell phenotypes overall. RFLP fingerprinting of Salmonella utilizing probes from ribosomal DNA, insertion sequence IS200, or random sequences has been reported. Ribotypes detected with ribosomal probes have generally been shared among different serotypes, whereas IS200 profiles have tended to be more serotype-specific. AP PCR and rep-PCR primers have been shown to discriminate Salmonella isolates, but fingerprints have been more difficult to reproduce and interpret than RFLP fingerprints. Several authors have reported bands of varying intensities, and some faint bands have not been reproducible. Improved methods of resolving and detecting PCR products are necessary. In a laboratory study, 85 environmental Salmonella isolates belonging to 22 serotypes were fingerprinted by 16S RFLP ribotyping, by rep-PCR, using ERIC (enterobacterial repetitive intergenic consensus) primers, and by AP PCR. Ribotypes were shared by isolates from different serotypes. ERIC PCR and one AP PCR primer produced fingerprints that discriminated among the different isolates, but did not identify serotypes. Another AP PCR primer produced simple patterns that neither discriminated isolates, nor identified serotypes. In a second related laboratory study, computer-assisted matching of AP PCR fingerprints of several known isolates was evaluated. Aliquots of the PCR reaction were run in the same and different gels, and the fingerprints bands were scored by two technicians on a presence-absence basis, and matched by creating dendrograms. Although replicate fingerprints of an isolate appeared reproducible, they were not always scored identically. Thus, the computer was not always able to correctly match fingerprints.

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24

Bischof, Laura Louise. "DNA fingerprinting analysis of captive Asian elephants, Elephas maximas". PDXScholar, 1990. https://pdxscholar.library.pdx.edu/open_access_etds/3966.

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This thesis examined the effectiveness of DNA fingerprinting analysis for paternity ascertainment and the establishment of relatedness of captive Asian elephants (Elephas maximas). Eighteen Asian elephants from three North .American zoos were examined. Thirteen of these elephants were wild caught. Relationships between these elephants and the remaining elephants born in captivity were known.

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25

Van, Ijperen Antonia Cornelia. "Fingerprinting and characterisation of Escherichia coli isolates using DNA arrays". Thesis, University of Greenwich, 2005. http://gala.gre.ac.uk/6329/.

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Two commercially available DNA whole genome Escherichia coli K12 arrays were compared to identify a subset of markers for typing. The arrays were identical in probe composition but different in substrate (membrane and glass slide arrays) and probe preparation (radio- and fluorescent-labelled). Labelled genomic E. coli DNA from five strains of the E. coli reference (ECOR) collection (ATCT35320 - ATCX35324) and E. coli K12 were hybridised against these arrays. A group of 1240 putative markers was identified on the membrane arrays and 649 were found on the glass slide arrays. Only a small proportion of these sequences (8%) was found through both platforms. Variability in the hybridisation signals from duplicate experiments made it difficult to identify useful markers. In order to investigate whether this technology could be used for characterising or typing E. coli strains, an array for the detection of 29 pathogenicity markers in E. coli strains was produced. This array was used with eight reference strains, including different pathotypes, 72 strains from the ECOR collection, and 49 clinical isolates. A wide range of E. coli pathogenicity markers was detected. The pathogenicity markers that were most common include chuA and iucC, which are both involved in iron metabolism. Additionally, the clinical isolates were grouped into clusters different from groupings based on biochemical tests. This demonstrates that the use of pathogenicity array typing can complement diagnostic tests on clinical E. coli isolates. An extended, second-generation, pathogenicity marker array containing 75 probes was made. The extended array successfully distinguished between ten closely related isolates from an outbreak of urinary tract infections, while previous tests were unable to do so. This array has the potential for providing a rapid and novel means of characterising pathogenic isolates.

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26

Appuhamy, Sarah. "DNA fingerprinting of Haemophilus somnus, Histophilus ovis and Actinobacillus seminis". Thesis, University of Glasgow, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.266458.

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27

Stacey, Glyn Nigel. "A study of the molecular genetic stability and diversity of animal cells". Thesis, Open University, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.294828.

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28

Armour, John Anthony. "The isolation and characterization of human minisatellite loci". Thesis, University of Leicester, 1990. http://hdl.handle.net/2381/35423.

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Abstract (sommario):

Hypervariable minisatellite regions of human DNA are of considerable interest, not only as highly informative genetic systems, but also as intermediately sized regions of tandem repetition. Methods for the isolation of minisatellite loci from the human genome have been investigated, and 23 new hypervariable loci cloned from an ordered array Charomid library. This method not only allows very efficient isolation of human minisatellites, but can also be used to observe the degree of overlap between multi-locus DNA fingerprinting probes. The 23 new loci have a mean heterozygosity level of 71%, and have been characterized and mapped in the genome. The genomic disposition of human minisatellites has been analysed by investigation of cloned examples. The minisatellites studied show a strong tendency to cluster near the ends of chromosomes, and sequence analysis demonstrates a significant excess of dispersed repeat elements in the DNA flanking human minisatellites. Minisatellite variant repeat (MVR) mapping has also been used to investigate the internal structure of minisatellite alleles. Somatic allele length mutation events have been demonstrated in DNA from colorectal adenocarcinomas, and the mutations observed show many features of general similarity to germline mutation events. A series of human breast tumours has been screened for somatic change, using both multi-locus DNA fingerprinting probes and single-locus minisatellite probes. Somatic change in breast cancers is much less frequent than in colorectal tumours, but some allele losses and mutations have been defined, including a highly unusual mutation, which may be the result of a minisatellite transposition event. Finally, evolution at minisatellite loci has been studied, both by examination of allelic states in current human populations, as well as comparison with non-human primates.

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29

Dean, Kristina. "Degradability of both a physical latent fingerprint and its associated extracted DNA". [Cedar City, Utah] : Southern Utah University, 2009. http://unicorn.li.suu.edu/ScholarArchive/ForensicScience/DeanKristina.pdf.

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30

Choy, Yan-tsun, e 蔡恩浚. "Statistical evaluation of mixed DNA stains". Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2009. http://hub.hku.hk/bib/B42664287.

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31

Chan, May-ngor. "Isolation and characterization of repetitive DNA sequences and their use in DNA fingerprinting and the population genetics of Perna viridis (L.) (Bivalvia : Mytilidae) /". Hong Kong : University of Hong Kong, 1997. http://sunzi.lib.hku.hk/hkuto/record.jsp?B19470423.

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32

Choudhury, Arpita. "The use of microsatellite DNA fingerprinting for aquaculture and fisheries science /". View online ; access limited to URI, 2005. http://0-wwwlib.umi.com.helin.uri.edu/dissertations/dlnow/3186898.

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33

Goll, Melanie. "Nachweis und DNA-Fingerprinting von Escherichia-coli-O157-Stämmen bei Pferden". Wetteberg : VVB Laufersweiler, 2005. http://deposit.ddb.de/cgi-bin/dokserv?idn=976072742.

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34

Ashworth, David. "The application of DNA fingerprinting to the conservation of threatened species". Thesis, University of Nottingham, 1992. http://eprints.nottingham.ac.uk/29183/.

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Abstract (sommario):

The human polycore minisatellite probes 33.6 and 33.15 developed by Prof. Alec Jeffreys and colleagues have been shown to detect hypervariable minisatellites in many taxonomically dispersed species. The mRNA derivatives of these two probes, pSPT19.6 and pSPT18.15, have here been used to probe the genomes of four species currently maintained in captivity. The wild populations of these species, Rothschild's mynah, the Rodrigues fruit bat, the British Merlin and the New Zealand falcon, are threatened with extinction to varying degrees. By using the technique of DNA fingerprinting, it has been possible to assess the levels of minisatellite variation remaining in these stocks, to confirm or refute the parent/offspring allocations made within, and in the case of Rothschild's mynah, to demonstrate that at least two of the founders of the stock were closely related. In addition, it has been possible to show that there is a significant positive relationship between the similarity coefficient calculated between two adults and the inbreeding coefficient calculated for their offspring.

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35

Ramakrishna, W. "DNA fingerprinting and molecular characterization of microsatellites and minisatellites in rice". Thesis(Ph.D.), CSIR-National Chemical Laboratory, Pune, 1994. http://dspace.ncl.res.in:8080/xmlui/handle/20.500.12252/2817.

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36

Kremer, Stefanie Lee. "The use of miniSTRS and mitochondrial DNA to identify handlers of pipe bombs". Diss., Connect to online resource - MSU authorized users, 2008.

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37

Morrison, Donald. "Evaluation of DNA typing methods for Enterococcus faecium". Thesis, University of London, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.312043.

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38

Noyes, H. A. "PCR based approaches to the identification and classification of Leishmania". Thesis, University of Liverpool, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.309913.

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Abstract (sommario):

Random amplified polymorphic DNA (RAPD) was tested for the identification and classification of Leishmania. RAPD was found to be useful for the identification of species of L. (Leishmania) and L. (Yiannia) and for the classification of L. (Yiannia) species. The polymerase chain reaction (PCR) was tested for the identification of Leishmania from mammals and lizards, using both published primers and new primers which amplify kinetoplast minicircle DNA. The size of the PCR product was found to be useful for discriminating between some sympatric pairs of species such as L. braziliensis and L. mexicana. Isotopically labelled probes prepared from the variable region of the kinetoplast minicircle were tested for specificity for the identification of New and Old World species of Leishmania. The specificity was dependent on the concentration of target DNA and was manipulated to investigate relationships between Leishmania species. Restriction digests of kinetoplast DNA (schizodemes) prepared by PCR and by centrifugation through 20% sucrose were compared for the identification of strains of L. infantum and L. chagasi. Twenty three strains of L. chagasi from cases of visceral and cutaneous leishmaniasis in Honduras were examined by RAPD, schizodemes, differential display, isoenzyrnes, RFLPs and PFGE to discover whether genetic differences existed between parasites causing the two different pathologies. The parasites were found to be unusually homogeneous and no differences were found which correlated with pathology by any of these methods. Restriction digests of PCR amplified small subunit ribosomal DNA (SSU rDNA) (ribodemes) were tested to find markers specific for the genus Leishmania. A classification of the Leishmania based on the restriction fragments indicated that L. hertigi and L. herreri were more closely related to Endotrypanum than to Leishmania, and that the lizard Leishmania could not be placed in separate genus from the Leishmania. Ribodemes were used to identify two strains of parasites supplied by colleagues in Central America that could not be identified by existing methods for the identification of Leishmania. One of these strains appeared to be identical to a C. luciliae reference strain. The other strain produced a fingerprint unlike any of the available reference strains. A variable region of the SSU rRNA gene was identified that was suitable for classifying trypanosomatids and the sequence of this region was used to classify the strain that could not be identified by fingerprinting.

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39

陳美娥 e May-ngor Chan. "Isolation and characterization of repetitive DNA sequences and their use in DNA fingerprinting and the population genetics of Perna viridis(L.) (Bivalvia : Mytilidae)". Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 1997. http://hub.hku.hk/bib/B31214964.

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40

Soleimani, Abdulvahab D. "Genetic diversity estimates and DNA fingerprinting of Canadian cultivars of durum wheat". Thesis, University of Ottawa (Canada), 2001. http://hdl.handle.net/10393/6192.

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Abstract (sommario):

Estimates of genetic diversity present in gene pools have important implications for breeders and germplasm curators. They constitute the raw material for plant improvement and can provide protection against genetic vulnerability to biotic and abiotic stresses. The purpose of this thesis was to derive Pedigree and Amplified Restricted Fragment Polymorphism (AFLP)-based Genetic Diversity Estimates (GDEPED and GDEAFLP, respectively) among all currently registered 13 Canadian durum wheat cultivars in order to test the hypothesis that the actual level of genetic variation at the DNA level is lower than what is measured from pedigree data. Two objectives were set in this thesis. The first objective was to estimate the genetic diversity level among cultivars using two independent methods namely AFLP and pedigree. The second objective of this thesis was to use cultivar-specific AFLP markers for identification of cultivars and transform these markers into sequence tagged site (STS) markers for routine identification of durum wheat cultivars. (Abstract shortened by UMI.)

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41

Li, Suiyang. "Establishment of an inbreeding index in Holstein dairy cattle using DNA fingerprinting". Thesis, McGill University, 1993. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=69763.

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In order to establish a method of assessing the degree of inbreeding within herds of cattle, we constructed a calibration index relating kinship and the degree of DNA band sharing in DNA fingerprints. Firstly, chickens were used as a model system to test the possibility of using microsatellite DNA as a probe for DNA fingerprinting in inbreeding analysis. Six genetic groups of chickens with estimated coefficients of inbreeding ranging from 0.026 to $>$ 0.98 (pedigree analysis) were fingerprinted using the minisatellite probe derived from M13 and the microsatellite probe (CAC)$ sb5$. The degree of band sharing using either probe increased in concert with the known amount of inbreeding and was described by the equation Y = 0.56X ($ pm$0.06) + 0.42 ($ pm$0.03); r = 0.998. Since in-gel hybridization using the microsatellite probes was faster and less labour intensive than using the minisatellite probe, it was used in the subsequent studies. Pedigree analysis in Holstein dairy cattle allowed for the empirical calibration of the association of band sharing with the coefficient of relatedness, (r), defined as the expected proportion of genes in 2 individuals that are identical by descent (i.e. for monozygous twins r = 1; for first order relatives r = 0.5; for half sibs r = 0.25 etc.). The average band sharing between pairs (6 pairs at each r value) of individuals within each class formed the basis for calibration. DNA was digested using RsaI. The relationship between band sharing and relatedness was well represented by a linear approximation Y = 0.51X ($ pm$0.09) + 0.50 ($ pm$0.04); r = 0.992. Using this calibration curve, random samples of animals within herds can be tested to establish the herd variability and to minimize inbreeding.

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42

Basiran, Mohd Nazir. "DNA insertion mutagenesis in higher plants". Thesis, University of Leicester, 1988. http://hdl.handle.net/2381/35436.

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43

Woodburn, Mary Alice. "Random amplified polymorphic DNA (RAPD) analysis of Bacillus sphaericus". Thesis, This resource online, 1994. http://scholar.lib.vt.edu/theses/available/etd-07102009-040429/.

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44

Beatini, Salvatore J. "Using DNA fingerprinting to assess genetic structure of the vernal pool amphibian rana sylvatica". Link to electronic thesis, 2003. http://www.wpi.edu/Pubs/ETD/Available/etd-0428103-153026.

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Abstract (sommario):

Thesis (M.S.)--Worcester Polytechnic Institute.
Keywords: wood frog; vernal pool conservation; fragmented habitat; Rana sylvatica; DNA fingerprinting. Includes bibliographical references (p. 38-40).

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45

Williams, Timothy Roy. "Reeves' muntjac : a molecular genetic study of an invading species". Thesis, University of Kent, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.283343.

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46

Dawson, Robert J. G. "A molecular examination of some enigmatic birds". Thesis, University of Nottingham, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.335505.

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47

Ridley, Anne McAlpine. "Epidemiological typing of Listeria monocytogenes". Thesis, Open University, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.361592.

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48

Fernando, Raniero. "Brood parasitism and genetic parentage in Goldeneye ducks, an analysis using DNA fingerprinting". Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape11/PQDD_0027/MQ51602.pdf.

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49

Dawe, Yvonne M. "DNA fingerprinting : a tool for determining genetic variability and strain relationships in poultry". Thesis, McGill University, 1988. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=63909.

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50

Goll, Melanie [Verfasser]. "Nachweis und DNA-Fingerprinting von Escherichia-coli-O157-Stämmen bei Pferden / Melanie Goll". Wetteberg : VVB Laufersweiler, 2005. http://d-nb.info/976072742/34.

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