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Letteratura scientifica selezionata sul tema "DNA fingerprinting of plants"

Autore: Grafiati

Pubblicato: 4 giugno 2021

Ultima modifica: 30 luglio 2024

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Articoli di riviste sul tema "DNA fingerprinting of plants"

1

Larson, S. "Plant Genotyping: The DNA Fingerprinting of Plants". Heredity 88, n. 3 (marzo 2002): 220. http://dx.doi.org/10.1038/sj.hdy.6800054.

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2

Cerny, Teresa A., e Terri W. Starman. "Molecular Phylogeny and DNA Amplification Fingerprinting of Petunia". HortScience 30, n. 4 (luglio 1995): 777F—778. http://dx.doi.org/10.21273/hortsci.30.4.777f.

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Seed of five species of petunia and 10 cultivars of Petunia xhybrida were obtained from several sources and plants were fingerprinted using DNA amplification fingerprinting (DAF). Within some species, variable fingerprints were generated between individual plants from the same seed source and/or different sources. Consistencies were found among DAF profiles by bulking the leaf tissue from 10 different plants, but not five plants. Each of 10 octamer primers used during the study revealed polymorphic loci between the species and cultivars. Among the 201 bands produced, 146 (73%) loci were polymorphic and these could be used to distinguish between each of the species and cultivars. Scoring for presence and absence of the amplified bands was used to generate a phylogenetic tree and to calculate the pairwise distances between each of the taxa using parsimony (PAUP) analysis. The tree generated using DAF molecular markers separated P. axillaris from P. parodii (two white-flowered species), and distinguished between the violet-flowered species, P, inflata, P. integrifolia, and P. violacea.

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Cerny, Teresa A., e Terri W. Starman. "Molecular Phylogeny and DNA Amplification Fingerprinting of Petunia". HortScience 30, n. 4 (luglio 1995): 777F—778. http://dx.doi.org/10.21273/hortsci.30.4.777.

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Abstract (sommario):

Seed of five species of petunia and 10 cultivars of Petunia xhybrida were obtained from several sources and plants were fingerprinted using DNA amplification fingerprinting (DAF). Within some species, variable fingerprints were generated between individual plants from the same seed source and/or different sources. Consistencies were found among DAF profiles by bulking the leaf tissue from 10 different plants, but not five plants. Each of 10 octamer primers used during the study revealed polymorphic loci between the species and cultivars. Among the 201 bands produced, 146 (73%) loci were polymorphic and these could be used to distinguish between each of the species and cultivars. Scoring for presence and absence of the amplified bands was used to generate a phylogenetic tree and to calculate the pairwise distances between each of the taxa using parsimony (PAUP) analysis. The tree generated using DAF molecular markers separated P. axillaris from P. parodii (two white-flowered species), and distinguished between the violet-flowered species, P, inflata, P. integrifolia, and P. violacea.

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4

Ahmad, Waqar, Khushi Muhammad, Altaf Hussain, Habib Ahmad, Khalid Kahn, Iqbal Ahmed Qarshi, Kamran Iqbal Shinwari et al. "DNA Fingerprinting of Essential Commercialized Medicinal Plants from Pakistan". American Journal of Plant Sciences 08, n. 09 (2017): 2119–32. http://dx.doi.org/10.4236/ajps.2017.89142.

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5

Munthali, M., B. V. Ford-Lloyd e H. J. Newbury. "The random amplification of polymorphic DNA for fingerprinting plants." Genome Research 1, n. 4 (1 maggio 1992): 274–76. http://dx.doi.org/10.1101/gr.1.4.274.

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6

Anastassopoulos, Elias. "DNA Fingerprinting in Plants. Principles, Methods, and Applications, Second edition". Economic Botany 60, n. 1 (aprile 2006): 97. http://dx.doi.org/10.1663/0013-0001(2006)60[97:dfippm]2.0.co;2.

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7

Rice, L. J., G. D. Ascough, J. F. Finnie e J. Van Staden. "DNA fingerprinting of Plectranthus plants for protection of cultivar registration". South African Journal of Botany 76, n. 2 (aprile 2010): 401. http://dx.doi.org/10.1016/j.sajb.2010.02.040.

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8

Chiang, Yu-Chung, Chang-Hung Chou, Shong Huang e Tzen-Yuh Chiang. "Possible consequences of fungal contamination on the RAPD fingerprinting in Miscanthus (Poaceae)". Australian Journal of Botany 51, n. 2 (2003): 197. http://dx.doi.org/10.1071/bt02021.

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Fungal contamination has been frequently reported in higher plants. In Miscanthus species, a wide range of fungal flora has also been recorded previously, including an investigation based on nrITS amplification. In order to understand the effects of the fungal genomes on the random amplified polymorphic DNA (RAPD) fingerprinting, callus specimens were obtained from the tissue culture of shoot apices of Miscanthus. RAPD fingerprinting with 60 oligoprimers was conducted with genomic DNA extracted from leaf tissue collected in the field and from the greenhouse, as well as callus derived from the same individuals. Extra bands were detected in the RAPD fingerprints amplified with 44 primers (84.6%) from the genomic DNA of both the field and greenhouse leaf tissue of most Miscanthus taxa examined, except for M. sinensis var. condensatus. Positive PCR amplification of organelle DNA non-coding spacers with both leaf and callus DNA ruled out the possibility that such DNA fingerprinting discrepancies were due to loss of organelles in the callus after consecutive subcultures. Among the 44 primers, one yielded no amplified fragments from the callus DNA, indicating that the amplified DNA fragments from leaf-tissue DNA were likely to be derived from fungi. The contaminating fungal DNA not only caused the overestimation of genetic diversity in the host plants, but also interfered with the phylogenetic inference. Systematic inconsistency occurred between the UPGMA dendrograms of leaf and callus DNA fingerprints. The detection of contaminating fungal DNA suggested that precautions are required for PCR-based fingerprinting when field materials are used for DNA resources. A method for quick screening of the contaminating fungal DNA with universal primers for the nrITS (internal transcribed spacer) region is suggested.

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Zhang, Donglin, Michael A. Dirr e Robert A. Price. "Application of DNA Markers to the Identification of Horticultural Plants". HortScience 32, n. 3 (giugno 1997): 534B—534. http://dx.doi.org/10.21273/hortsci.32.3.534b.

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The correct identification of horticultural taxa becomes more and more important for intellectual property protection and economic reasons. Traditionally, morphological characteristics have been used to differentiate among the horticultural taxa. However, the morphological characteristics may vary with plant age, cultural conditions, and climate. Modern technologies, such as DNA markers, are now employed in the identification of horticultural taxa. Currently, technologies of DNA sequencing (gene sequences) and DNA fingerprinting (RAPD, RFLP, SSR, and AFLP) are available for distinguishing among horticultural taxa. The literature and our personal experience indicate that the application of each technique depends on the taxon and ultimate goal for the research. DNA sequencing of a variety of nuclear or chloroplast encoded genes or intergenic spacers (rbcL, ndhF, matK, ITS) can be applied to distinguish different species. All DNA fingerprinting technologies can be used to classify infraspecies taxa. AFLP (the most modern technique) is the better and more-reliable to identify taxa subordinate to the species, while RAPDs can be employed in clonal or individual identification. Techniques of RFLP and SSR lie between AFLP and RAPD in their effectiveness to delineate taxa. Mechanics, laboratory procedures, and inherent difficulties of each technique will be briefly discussed. Application of the above technologies to the classification of Cephalo taxus will be discussed in concert with the morphological and horticultural characteristics. Future classification and identification of horticultural taxa should combine DNA technology and standard morphological markers.

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Rao, R., G. Corrado, M. Bianchi e A. Di Mauro. "(GATA)4 DNA fingerprinting identifies morphologically characterized 'San Marzano' tomato plants". Plant Breeding 125, n. 2 (aprile 2006): 173–76. http://dx.doi.org/10.1111/j.1439-0523.2006.01183.x.

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Tesi sul tema "DNA fingerprinting of plants"

1

Ho, Siu-hong. "Isolation and characterization of Panax Ginseng repetitive DNA sequences for DNA fingerprinting /". Hong Kong : University of Hong Kong, 1998. http://sunzi.lib.hku.hk/hkuto/record.jsp?B19737816.

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何兆康 e Siu-hong Ho. "Isolation and characterization of Panax Ginseng repetitive DNA sequences for DNA fingerprinting". Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 1998. http://hub.hku.hk/bib/B31215282.

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Morin, Geneviève. "Metabolite fingerprinting tools to detect differences between transgenic and conventional crops". Thesis, McGill University, 2007. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=101629.

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A concern in transgenic crops is the potential risk posed by unintended effects which could result from genetic transformation. The objective of this work was to develop an untargeted approach that could characterize transgenic crops, as well as conventional crops, at the molecular level. An experimental approach was designed and used to compare conventional and transgenic soybean varieties. Varieties were compared using their metabolite fingerprints obtained by reverse-phase high performance liquid chromatography (HPLC) and both the analytical and biological variability were assessed. Multivariate and univariate statistical analyses were applied to the data to detect significant differences between the varieties. It was found that transgenic variety PS 46 RR was the most different variety analyzed and that it differed most from Mandarin (Ottawa) and AC Dundas. The statistical analyses also determined that PS 46 RR differed more from the conventional varieties tested than 2601R did.

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Cheung, Kin Lok. "Investigation of (3-mercaptopropyl) trimethoxysilane (MPTS)-modified surface and DNA microarray for genotyping of traditional Chinese medicinal plants /". View Abstract or Full-Text, 2003. http://library.ust.hk/cgi/db/thesis.pl?BIOL%202003%20CHEUNGK.

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Thesis (M. Phil.)--Hong Kong University of Science and Technology, 2003.
Includes bibliographical references (leaves 103-111). Also available in electronic version. Access restricted to campus users.

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Honing, Jennifer. "Evaluation and implementation of DNA-based diagnostic methodology to distinguish wheat genotypes". Thesis, Link to the online version, 2007. http://hdl.handle.net/10019/638.

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Micha, Caterina. "Establishment of phylogenetic relationships within the genus Phragmipedium using RAPD-PCR fingerprinting". Virtual Press, 1995. http://liblink.bsu.edu/uhtbin/catkey/958787.

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DNA fingerprinting was applied for the molecular elucidation of taxonomic relationships within a genus of orchids which have previously been based on morphological characteristics. Phragmipediwn consists of 15-20 species native to Central and South America. This research project included two studies. In the first study DNA was isolated from 11 samples (including two unidentified ones). These individuals, which were mostly hybrids, were found in the Wheeler Orchid Collection and Species Bank at Ball State University. In order to position Phragmipediwn within the orchid family fingerprinting was also performed on individuals in the sister taxa, Cypripedium and Paphiopedium, which are members of the same subfamily, and on a member of the outgroup taxon Vanda. The polymerase chain reaction (PCR) was employed to yield fingerprints resulting from the use of random primers. Fifty nine random amplified polymorphic DNA (RAPD) bands were obtained using 5 different primers to yield 107 polymorphic bands. As many as 75% of genetic loci were found to be shared between hybrids that resulted from a cross of more than one individual in the same section. However the percentage dropped to 35-65 % when only one parent was shared in the cross. Furthermore, the sister group taxa Cypripedium and Paphilopedium shared from 12 % -35 % of their polymorphic loci with the members of the genus Phragmipedium. The outgroup taxon Vanda shared 17% of its polymorphic loci with the rest of the samples.In a second study DNA was isolated from one member of each of the five sections of the genus Phragmipedium, and RAPD-PCR fingerprinting was used to compare their genetic similarities to that of the two sister taxa and the outgroup taxon. It was found that individuals in different genera shared 25% or less of their polymorphic bands. Between sections of the same genus 20-50% of genetic loci were shared. Two sections, Platypetalwn and Phragmipedium showed the highest degree of genetic relatedness (41-53%). Again the outgoup taxon shared less than 20% Phragmipediwn samples on the phenograms produced but the percentage was again insignificant. However, genetic analyses of the members of the section Lorifolia gave conflicting results: 46% genetic identity was observed in the first trial and 20% in the second.In conclusion, RAPD-PCR fingerprinting results appeared to be effective in the positioning of sections within a genus indicating the degree of similarity of closely related taxa. Also RAPD-PCR was able to place an unknown individual within a specific section of the genus. However, it could not be employed to determine the identity of unknown species due to the high degree of genetic diversity observed between even closely related individuals.
Department of Biology

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7

Zhang, Yanbo. "Molecular approach to the authentication of lycium barbarum and its related species". HKBU Institutional Repository, 2000. http://repository.hkbu.edu.hk/etd_ra/227.

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Saxon, Herbert. "The molecular biology of orchids : transformation by Agrobacterium Tumefaciens and DNA fingerprinting". Virtual Press, 1995. http://liblink.bsu.edu/uhtbin/catkey/941575.

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The work reported here was done at the Wheeler Orchid Collection and Species Bank and the Department of Biology at Ball State University. We have developed a research teaching program with two applied research goals: genetically transforming and DNA fingerprinting orchid tissue. As part of their molecular biology education, students have investigated the genetic transformation of orchids for mitigating viral symptoms and the identification of unknown orchids by DNA fingerprinting. In a second application of the technology, DNA fingerprinting has been used to determine evolutionary relationships and to quantify genetic diversity among orchids.This dissertation details the background and need for this project and the research that was done to start it. As the early work has, developed and students have added their contributions, the data have developed into two papers formatted for submission to scientific journals. They are included as results.The first is a project designed to insert exognenous DNA into orchid tissue. The soil microbe Agrobacterium tumefaciens causes crown-gall tumors to develop in its plant hosts by inserting DNA into their cells which then controls the biosynthesis of development-controlling hormones. A. tumefaciens which has been disarmed has been routinely used to bioengineer dicotyledonous plants but its use has been rare on monocotyledons. In this paper, we report that A. tumefaciens transformed embryonic orchid tissue and caused alteration in its normal developmental course.The second paper details the DNA fingerprinting of tissue from Aplectrum hymale, a terrestrial orchid native to this climate. Three populations of A. hymale have been sampled and DNA extracted from the tissue samples. RAPD primers were used to prime PCR amplifications of random sequences of the DNA and the amplified DNA was visualized by gel electrophoresis. Loci of the resulting bands were treated as potentially multiallelic gene loci and heterozygosity between and within subpopulations was calculated. We report that the three populations could be partially differentiated by this procedure and that the two populations located nearest to each other yielded the least between -ubpopulation heterozygosity. We report very high levels of genetic diversity between individuals within small subpopulations in spite of the fact that these subpopulations are considered to be primarily clonal in reproductive nature.
Department of Biology

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9

Nogueira, Lia. "Non-tariff barriers and technology trade and welfare implications /". Online access for everyone, 2008. http://www.dissertations.wsu.edu/Dissertations/Summer2008/l_nogueira_072308.pdf.

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Rinaldi, Catherine. "Authentication of the Panax genus plants used in Traditional Chinese Medicine (TCM) using Randomly Amplified Polymorphic DNA (RAPD) analysis". University of Western Australia. Centre for Forensic Science, 2007. http://theses.library.uwa.edu.au/adt-WU2008.0054.

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[Truncated abstract] Traditional medicines are used by millions of people throughout the world as their primary source of medical care. A range of materials are in used traditional medicines including plant and animal parts. Even though the traditional medicine trade is estimated to be worth sixty billion dollars annually the trade remains largely unregulated. Unscrupulous practices by vendors to increase their profit margins such as substituting and adulterating expensive material with cheaper varieties go unchecked. This can be dangerous to consumers because some substitutions involve poisonous material. Also, animal parts from endangered species can find their way into traditional medicines, therefore there needs to be a way to identify them in traditional medicines to prosecute poachers. The traditional techniques used for the identification of material used in Traditional Chinese Medicine (TCM) include, morphological, histological, chemical and immunological analysis. However, these techniques have their limitations. This makes applying multiple techniques essential to provide thorough authentication of the material. DNA profiling provides a technique well suited to analysing material used in TCM. DNA profiling is advantageous over other techniques used to authenticate material used in TCM because it requires only a small sample amount, can determine the cultivator, be used on all forms of TCM and potentially distinguish the components of mixtures. ... Therefore, profiles of different species/individual are different and species? can be distinguished. Commercially sold traditional medicines are processed which is likely to degrade the DNA of the sample making extraction and amplification difficult. Here an organic Phenol:Chloroform extraction technique extracted DNA from commercial dried root samples. The extracted DNA was amplifiable using RAPD primers. The RAPD primers used here produced enough polymorphic bands to distinguish different plant species. They were used to distinguish commercial samples that were sold as three different species within the Panax genus, Panax ginseng, Panax quinquefolium and Panax notoginseng and genetically unrelated plant material; Potato and Eleutherococcus senticosus. Liquid samples and mixtures were also profiled with the RAPD primers to determine whether the RAPD primers provide enough distinguishing ability to analyse these forms of TCM. DNA was extracted from the liquid samples, one a ginseng drink and the other an ginseng extractum. However, there was no reliability in the production of PCR products. The analysis of the mixture samples found that not enough polymorphic bands were produced by the RAPD primers used here to identify Panax species within mixtures of two Panax species. While when P. ginseng was mixed with a genetically unrelated sample there was enough polymorphism to differentiate the two samples in the mixture. The results of this research show that RAPD analysis provides a simple and inexpensive technique to begin analysis of materials used in TCM. Using RAPD analysis it is possible to distinguish Panax plant species from each other. However, the RAPD primers used here did not provide enough reproducibility or polymorphism to analyse liquid and mixtures of Panax species plants.

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Libri sul tema "DNA fingerprinting of plants"

1

Kurt, Weising, a cura di. DNA fingerprinting in plants and fungi. Boca Raton: CRC Press, 1995.

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2

Henry, R. J., a cura di. Plant genotyping: the DNA fingerprinting of plants. Wallingford: CABI, 2001. http://dx.doi.org/10.1079/9780851995151.0000.

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J, Henry Robert, a cura di. Plant genotyping: The DNA fingerprinting of plants. Wallingford, UK: CABI Pub., 2001.

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Kurt, Weising, a cura di. DNA fingerprinting in plants: Principles, methods, and applications. 2^a ed. Boca Raton, FL: Taylor & Francis Group, 2005.

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Lakshmikumaran, Malathi. DNA fingerprinting of medicinal plants: A means to preserve valuable genetic resources. [New Delhi]: Rajiv Gandhi Institute for Contemporary Studies, 1996.

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6

Research Co-ordination Meeting on the Use of Novel DNA Fingerprinting Techniques for the Detection and Characterization of Genetic Variation in Vegetatively Propagated Crops (3rd 1997 Mumbai, India). Use of novel DNA fingerprinting techniques for the detection and characterization of genetic variation in vegetatively propagated crops: Proceedings of a final Research Co-ordination Meeting organized by the Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture and held in Mumbai, India, 24-28 February 1997. Vienna, Austria: International Atomic Energy Agency, 1998.

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White, Linda J. Seeds of evidence. Nashville, Tenn: Abingdon Press Fiction, 2013.

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Boronnikova, S. V. Molekuli︠a︡rno-geneticheskai︠a︡ identifikat︠s︡ii︠a︡ i pasportizat︠s︡ii︠a︡ redkikh i nakhodi︠a︡shchikhsi︠a︡ pod ugrozoĭ ischeznovenii︠a︡ vidov rasteniĭ. Permʹ: Permskiĭ gos. universitet, 2009.

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Zhang, Chao Mei. Genotyping of Solanum tuberosum ssp. tuberosum L. Dublin: University College Dublin, 1998.

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Satō, Yōichirō. Yomigaeru midori no Shirukurōdo: Kankyō shigaku no susume. Tōkyō: Iwanami Shoten, 2006.

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Capitoli di libri sul tema "DNA fingerprinting of plants"

1

Cooke, David. "DNA Fingerprinting Techniques". In Fungal Plant Pathogens, 197–207. 2^a ed. GB: CABI, 2023. http://dx.doi.org/10.1079/9781800620575.0065.

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Stewart, C. Neal. "Rapid DNA Extraction from Plants". In Fingerprinting Methods Based on Arbitrarily Primed PCR, 25–28. Berlin, Heidelberg: Springer Berlin Heidelberg, 1997. http://dx.doi.org/10.1007/978-3-642-60441-6_4.

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Saumitou-Laprade, Pierre, Yves Piquot, Olivier Raspé, Jacqueline Bernard e Klaas Vrieling. "Plant DNA Fingerprinting and Profiling". In DNA Profiling and DNA Fingerprinting, 17–38. Basel: Birkhäuser Basel, 1999. http://dx.doi.org/10.1007/978-3-0348-7582-0_2.

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Sucher, Nikolaus J., James R. Hennell e Maria C. Carles. "DNA Fingerprinting, DNA Barcoding, and Next Generation Sequencing Technology in Plants". In Methods in Molecular Biology, 13–22. Totowa, NJ: Humana Press, 2012. http://dx.doi.org/10.1007/978-1-61779-609-8_2.

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Karihaloo, J. L. "DNA Fingerprinting Techniques for Plant Identification". In Plant Biology and Biotechnology, 205–21. New Delhi: Springer India, 2015. http://dx.doi.org/10.1007/978-81-322-2283-5_9.

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Nybom, H. "Applications of DNA fingerprinting in plant population studies". In DNA Fingerprinting: State of the Science, 293–309. Basel: Birkhäuser Basel, 1993. http://dx.doi.org/10.1007/978-3-0348-8583-6_27.

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Nybom, H. "Applications of DNA Fingerprinting in Plant Breeding". In Experientia Supplementum, 294–311. Basel: Birkhäuser Basel, 1991. http://dx.doi.org/10.1007/978-3-0348-7312-3_21.

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Nybom, Hilde. "DNA fingerprinting — A useful tool in fruit breeding". In Developments in Plant Breeding, 257–62. Dordrecht: Springer Netherlands, 1994. http://dx.doi.org/10.1007/978-94-011-0467-8_53.

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He, Qingyao, Jiayu Yao, Pingping Fang, Jianmin Qi e Liemei Zhang. "DUS Test and DNA Fingerprinting Construction of Jute Varieties". In Compendium of Plant Genomes, 65–79. Cham: Springer International Publishing, 2022. http://dx.doi.org/10.1007/978-3-030-91163-8_5.

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Wu, Chengcang, Shuku Sun, Mi-Kyung Lee, Zhanyou Xu, Chengwei Ren, Teofila S. Santos e Hong-Bin Zhang. "Whole-Genome Physical Mapping: An Overview on Methods for DNA Fingerprinting". In The Handbook of Plant Genome Mapping, 257–83. Weinheim, FRG: Wiley-VCH Verlag GmbH & Co. KGaA, 2005. http://dx.doi.org/10.1002/3527603514.ch11.

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Atti di convegni sul tema "DNA fingerprinting of plants"

1

Lee, Michael. "DNA Fingerprinting of Crop Germplasm". In Proceedings of the 1992 Crop Production and Protection Conference. Iowa State University, Digital Press, 1993. http://dx.doi.org/10.31274/icm-180809-450.

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Williams, McKay D., Sheldon A. Munns, Michael A. Temple e Michael J. Mendenhall. "RF-DNA Fingerprinting for Airport WiMax Communications Security". In 2010 4th International Conference on Network and System Security (NSS). IEEE, 2010. http://dx.doi.org/10.1109/nss.2010.21.

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Peggs, Cinque S., Tanner S. Jackson, Ashley N. Tittlebaugh, Taylor G. Olp, Joshua H. Tyler, Donald R. Reising e T. Daniel Loveless. "Preamble-based RF-DNA Fingerprinting Under Varying Temperatures". In 2023 12th Mediterranean Conference on Embedded Computing (MECO). IEEE, 2023. http://dx.doi.org/10.1109/meco58584.2023.10155035.

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Chen, C. H. Winston, Kai Tang, N. I. Taranenko, S. L. Allman e L. Y. Ch'ang. "Laser mass spectrometry for DNA fingerprinting for forensic applications". In SPIE's 1994 International Symposium on Optics, Imaging, and Instrumentation, a cura di Richard J. Mammone e J. David Murley, Jr. SPIE, 1994. http://dx.doi.org/10.1117/12.191883.

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Clouting, C., E. Liebana, Robert H. Davies, L. Garcia-Migura e S. Bedford. "DNA fingerprinting of S. typhimurium from a pig longitudinal study". In Fifth International Symposium on the Epidemiology and Control of Foodborn Pathogens in Pork. Iowa State University, Digital Press, 2003. http://dx.doi.org/10.31274/safepork-180809-522.

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Cobb, William E., Eric W. Garcia, Michael A. Temple, Rusty O. Baldwin e Yong C. Kim. "Physical layer identification of embedded devices using RF-DNA fingerprinting". In MILCOM 2010 - 2010 IEEE Military Communications Conference. IEEE, 2010. http://dx.doi.org/10.1109/milcom.2010.5680487.

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Chen, C. H. Winston, N. I. Taranenko, Y. F. Zhu, C. N. Chung e S. L. Allman. "Laser mass spectrometry for DNA sequencing, disease diagnosis, and fingerprinting". In BiOS '97, Part of Photonics West, a cura di Gerald E. Cohn e Steven A. Soper. SPIE, 1997. http://dx.doi.org/10.1117/12.274339.

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8

Wilson, Aaron J., Donald R. Reising e T. Daniel Loveless. "Integration of Matched Filtering within the RF-DNA Fingerprinting Process". In GLOBECOM 2019 - 2019 IEEE Global Communications Conference. IEEE, 2019. http://dx.doi.org/10.1109/globecom38437.2019.9014225.

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Williams, McKay D., Michael A. Temple e Donald R. Reising. "Augmenting Bit-Level Network Security Using Physical Layer RF-DNA Fingerprinting". In GLOBECOM 2010 - 2010 IEEE Global Communications Conference. IEEE, 2010. http://dx.doi.org/10.1109/glocom.2010.5683789.

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Reising, Donald R., Michael A. Temple e Mark E. Oxley. "Gabor-based RF-DNA fingerprinting for classifying 802.16e WiMAX Mobile Subscribers". In 2012 International Conference on Computing, Networking and Communications (ICNC). IEEE, 2012. http://dx.doi.org/10.1109/iccnc.2012.6167534.

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Rapporti di organizzazioni sul tema "DNA fingerprinting of plants"

1

Echt, Craig, e Sedley Josserand. DNA fingerprinting sets for four southern pines. Asheville, NC: U.S. Department of Agriculture, Forest Service, Southern Research Station, 2018. http://dx.doi.org/10.2737/srs-rn-24.

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2

Echt, Craig, e Sedley Josserand. DNA fingerprinting sets for four southern pines. Asheville, NC: U.S. Department of Agriculture, Forest Service, Southern Research Station, 2018. http://dx.doi.org/10.2737/srs-rn-24.

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3

Gupta, Shweta. DNA Fingerprinting: A Major Tool for Crime Investigation. Spring Library, aprile 2021. http://dx.doi.org/10.47496/nl.blog.24.

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Abstract (sommario):

DNA profiling has revolutionized the criminal justice system over the past decades. It has even enabled the law enforcement from exonerating people who have been convicted wrongfully of crimes which they did not commit.

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Bischof, Laura. DNA fingerprinting analysis of captive Asian elephants, Elephas maximas. Portland State University Library, gennaio 2000. http://dx.doi.org/10.15760/etd.5850.

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5

Winston Chen, C. H., N. I. Taranenko, Y. F. Zhu, C. N. Chung e S. L. Allman. Laser mass spectrometry for DNA sequencing, disease diagnosis, and fingerprinting. Office of Scientific and Technical Information (OSTI), marzo 1997. http://dx.doi.org/10.2172/446348.

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6

Weil, Clifford F., Anne B. Britt e Avraham Levy. Nonhomologous DNA End-Joining in Plants: Genes and Mechanisms. United States Department of Agriculture, luglio 2001. http://dx.doi.org/10.32747/2001.7585194.bard.

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Abstract (sommario):

Repair of DNA breaks is an essential function in plant cells as well as a crucial step in addition of modified DNA to plant cells. In addition, our inability to introduce modified DNA to its appropriate locus in the plant genome remains an important hurdle in genetically engineering crop species.We have taken a combined forward and reverse genetics approach to examining DNA double strand break repair in plants, focusing primarily on nonhomologous DNA end-joining. The forward approach utilizes a gamma-plantlet assay (miniature plants that are metabolically active but do not undergo cell division, due to cell cycle arrest) and has resulted in identification of five Arabidopsis mutants, including a new one defective in the homolog of the yeast RAD10 gene. The reverse genetics approach has identified knockouts of the Arabidopsis homologs for Ku80, DNA ligase 4 and Rad54 (one gene in what proves to be a gene family involved in DNA repair as well as chromatin remodeling and gene silencing)). All these mutants have phenotypic defects in DNA repair but are otherwise healthy and fertile. Additional PCR based screens are in progress to find knockouts of Ku70, Rad50, and Mre11, among others. Two DNA end-joining assays have been developed to further our screens and our ability to test candidate genes. One of these involves recovering linearized plasmids that have been added to and then rejoined in plant cells; plasmids are either recovered directly or transformed into E. coli and recovered. The products recovered from various mutant lines are then compared. The other assay involves using plant transposon excision to create DNA breaks in yeast cells and then uses the yeast cell as a system to examine those genes involved in the repair and to screen plant genes that might be involved as well. This award supported three graduate students, one in Israel and two in the U.S., as well as a technician in the U.S., and is ultimately expected to result directly in five publications and one Masters thesis.

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Mullet, J. E. Regulation of chloroplast number and DNA synthesis in higher plants. Final report. Office of Scientific and Technical Information (OSTI), novembre 1995. http://dx.doi.org/10.2172/132689.

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8

Mullet, J. E. Regulation of chloroplast number and DNA synthesis in higher plants. Final report. Office of Scientific and Technical Information (OSTI), novembre 1995. http://dx.doi.org/10.2172/134990.

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9

Wilson, Thomas E., Avraham A. Levy e Tzvi Tzfira. Controlling Early Stages of DNA Repair for Gene-targeting Enhancement in Plants. United States Department of Agriculture, marzo 2012. http://dx.doi.org/10.32747/2012.7697124.bard.

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Abstract (sommario):

Gene targeting (GT) is a much needed technology as a tool for plant research and for the precise engineering of crop species. Recent advances in this field have shown that the presence of a DNA double-strand break (DSB) in a genomic locus is critical for the integration of an exogenous DNA molecule introduced into this locus. This integration can occur via either non-homologous end joining (NHEJ) into the break or homologous recombination (HR) between the broken genomic DNA and the introduced vector. A bottleneck for DNA integration via HR is the machinery responsible for homology search and strand invasion. Important proteins in this pathway are Rad51, Rad52 and Rad54. We proposed to combine our respective expertise: on the US side, in the design of zincfinger nucleases (ZFNs) for the induction of DNA DSBs at any desired genomic locus and in the integration of DNA molecules via NHEJ; and on the Israeli side in the HR events, downstream of the DSB, that lead to homology search and strand invasion. We sought to test three major pathways of targeted DNA integration: (i) integration by NHEJ into DSBs induced at desired sites by specially designed ZFNs; (ii) integration into DSBs induced at desired sites combined with the use of Rad51, Rad52 and Rad54 proteins to maximize the chances for efficient and precise HR-mediated vector insertion; (iii) stimulation of HR by Rad51, Rad52 and Rad54 in the absence of DSB induction. We also proposed to study the formation of dsT-DNA molecules during the transformation of plant cells. dsT-DNA molecules are an important substrate for HR and NHEJ-mediatedGT, yet the mode of their formation from single stranded T-DNA molecules is still obscure. In addition we sought to develop a system for assembly of multi-transgene binary vectors by using ZFNs. The latter may facilitate the production of binary vectors that may be ready for genome editing in transgenic plants. ZFNs were proposed for the induction of DSBs in genomic targets, namely, the FtsH2 gene whose loss of function can easily be identified in somatic tissues as white sectors, and the Cruciferin locus whose targeting by a GFP or RFP reporter vectors can give rise to fluorescent seeds. ZFNs were also proposed for the induction of DSBs in artificial targets and for assembly of multi-gene vectors. We finally sought to address two important cell types in terms of relevance to plant transformation, namely GT of germinal (egg) cells by floral dipping, and GT in somatic cells by root and leave transformation. To be successful, we made use of novel optimized expression cassettes that enable coexpression of all of the genes of interest (ZFNs and Rad genes) in the right tissues (egg or root cells) at the right time, namely when the GT vector is delivered into the cells. Methods were proposed for investigating the complementation of T-strands to dsDNA molecules in living plant cells. During the course of this research, we (i) designed, assembled and tested, in vitro, a pair of new ZFNs capable of targeting the Cruciferin gene, (ii) produced transgenic plants which expresses for ZFN monomers for targeting of the FtsH2 gene. Expression of these enzymes is controlled by constitutive or heat shock induced promoters, (iii) produced a large population of transgenic Arabidopsis lines in which mutated mGUS gene was incorporated into different genomic locations, (iv) designed a system for egg-cell-specific expression of ZFNs and RAD genes and initiate GT experiments, (v) demonstrated that we can achieve NHEJ-mediated gene replacement in plant cells (vi) developed a system for ZFN and homing endonuclease-mediated assembly of multigene plant transformation vectors and (vii) explored the mechanism of dsTDNA formation in plant cells. This work has substantially advanced our understanding of the mechanisms of DNA integration into plants and furthered the development of important new tools for GT in plants.

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Mullet, J. E. Regulation of chloroplast number and DNA synthesis in higher plants. Final report, August 1995--August 1996. Office of Scientific and Technical Information (OSTI), giugno 1997. http://dx.doi.org/10.2172/548678.

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