Tesi sul tema "Cryptosporidium"
Cita una fonte nei formati APA, MLA, Chicago, Harvard e in molti altri stili
Vedi i top-50 saggi (tesi di laurea o di dottorato) per l'attività di ricerca sul tema "Cryptosporidium".
Accanto a ogni fonte nell'elenco di riferimenti c'è un pulsante "Aggiungi alla bibliografia". Premilo e genereremo automaticamente la citazione bibliografica dell'opera scelta nello stile citazionale di cui hai bisogno: APA, MLA, Harvard, Chicago, Vancouver ecc.
Puoi anche scaricare il testo completo della pubblicazione scientifica nel formato .pdf e leggere online l'abstract (il sommario) dell'opera se è presente nei metadati.
Vedi le tesi di molte aree scientifiche e compila una bibliografia corretta.
Giles, Michaela. "Host specificity and molecular detection of Cryptosporidium hominis and Cryptosporidium parvum". Thesis, London School of Hygiene and Tropical Medicine (University of London), 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.433502.
Testo completoBenamrouz, Sadia. "Infection par Cryptosporidium spp. du modèle souris SCID traité à la dexaméthasone : caractérisation cellulaire et moléculaire du processus de cancérisation des épithéliums digestifs". Thesis, Lille 2, 2012. http://www.theses.fr/2012LIL2S040/document.
Testo completoCertad and col, showed recently that Cryptosporidium parvum is also capable of inducing gastrointestinal adenomas with intraepithelial neoplasia of low and high grade, and adenocarcinomas in situ in SCID mice (Severe Combined immunodeficiency), treated with dexamethasone (SCID-D). In addition, we also know that Cryptosporidium muris induces a chronic infection but no neoplastic lesions.This is why we decided first to determine the minimum dose of C. parvum (IOWA) which can infect and cause digestive neoplasia in this model. This work allowed us to conclude that one oocyst is able to induce in SCID-D mice not only a chronic infection but also the development of neoplastic lesions in both the antropyloric region of the stomach and the ileocecal region at 45 days post infection. We also followed up the progression of these lesions after infection with several doses of C. parvum strain IOWA (theoretically: 1, 10, 100 and 105 oocysts). This work was also performed after inoculation of another strain of different origin isolated from an immunosupressed patient suffering from a severe cryptosporidiosis after a near-drowning). To do this we have achieved: an extended follow-up of animals (over 84 days) and an histopathological analysis based on immunohistochemical detection of cytokeratin and alpha smooth actin. For the first time it was noted with all doses and for the two strains, in both the antropyloric and ileocecal region of animals, a patern characteristic of invasif adenocarcinoma: desmoplasia and buds of tumor cells invading the lamina propria. In addition to these histological features, a discontinuous basement membrane, the presence of epithelial cells in the stroma, an interruption of the muscularis mucosa and an invasion of the muscularis were also detected. In the case of the strain of C. parvum of human origin, the adenocarcinoma also invaded the serosa and epithelial cells were observed inside blood vessels (vascular tumor emboli). Lesion’s progression was so fast that after only 60 days post-infection we observed at least, the invasion of the submucosa at ileocecal region. Furthermore, the results of this study showed for the first time the ability of an isolate of C. parvum of human origin to cause chologiocarcinoma in an experimental model. Finally, using an immunohistochemical approach, we explored metabolic pathways involved in the development of C. parvum-induced neoplastic lesions at the ileocecal region. Four markers involved in major pathways altered in colorectal cancer were chosen: APC, beta-catenin, p53 and K-ras. The assesment of tumor marker expression in the ileocaecal area showed an abnormal localization of APC, beta-catenin and p53. Beta-catenin and p53 accumulated in the cytoplasm, while APC labelling decreased or even disappeared. Meanwhile, K-ras was still at membrane level as in normal cells. these results suggest the involvement of p53 and Wnt pathway in the phenomenon of carcinogenesis in our mouse model (SCID-D). Studies to search the implication of other markers and possible mutations of the genes encoding these proteins are underway. In conclusion,, these findings show that different strains of C. parvum including a strain of human origin induce digestif invasive adenocarcinomas whatever the inoculum size administered to SCID-D mice. These results confirm the role of C. parvum in the induction of digestive cancer in immunocompromised hosts. In addition, the pathways involved in the process of carcinogenesis in mice (SCID-D) appeared to be the same as those altered in humans. Moreover, the Wnt signaling pathway in which the actin polymerization and rearrangement of the cytoskeleton are involved is a major event during Cryptosporidium infection and appears to play a role in the carcinogenic process induced by the parasite
Siddiki, Amam Zonaed. "Proteome analysis of Cryptosporidium". Thesis, University of Liverpool, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.428228.
Testo completoArrowood, Michael James. "Cryptosporidium: Oocyst production and hybridoma generation for examining colostrum and monoclonal antibody roles in cryptosporidial infections". Diss., The University of Arizona, 1988. http://hdl.handle.net/10150/184335.
Testo completoPollok, Richard. "Cryptosporidium parvum : host-parasite interactions". Thesis, Queen Mary, University of London, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.402442.
Testo completoBuaprathoom, Somporn. "Photonics based cryptosporidium detection systems". Thesis, University of Surrey, 2012. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.580330.
Testo completoSilva, Deuvânia Carvalho da [UNESP]. "Avaliação física, epidemiológica e molecular da infecção por Cryptosporidium spp. em passeriformes". Universidade Estadual Paulista (UNESP), 2009. http://hdl.handle.net/11449/94711.
Testo completoFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Devido à carência de informações relacionadas à epidemiologia da infecção por Cryptosporidium spp. em passeriformes, neste trabalho objetivou-se determinar a periodicidade da eliminação fecal de oocistos de Cryptosporidium, os sinais clínicos, a presença de mortalidade, e a caracterização molecular desse coccídio. Foram colhidas 480 amostras de fezes, provenientes de 40 aves, sendo 372 amostras de 31 aves adultas e 108 amostras de nove filhotes até 12 semanas de vida, com periodicidade mensal, no período de setembro de 2007 a setembro de 2008, com exceção do mês de abril. As aves estavam alojadas em cinco criatórios, com criação de bicudo (Oryzoborus maximiliani), curió (Oryzoborus angolensis), azulão (Passerina brissonii) e coleira do brejo (Sporophila collaris). As amostras foram conservadas em bicromato de potássio 2,5%, a 4ºC, até o processamento. Os oocistos foram purificados por centrífugo-flutuação em solução de Sheather, seguindo-se a extração do DNA genômico dos oocistos e a classificação molecular, por meio da reação em cadeia de polimerase-nested, para amplificação de fragmentos da subunidade 18S do gene do RNA ribossômico. Eliminação fecal intermitente de Cryptosporidium spp. foi observada em 91 (24,5%) amostras de aves adultas, com maior ocorrência nos períodos que se aproximam dos períodos de muda de penas e de reprodução das aves e em 14 amostras (13%) de aves jovens. O sequenciamento dos fragmentos de DNA amplificados possibilitou a identificação de somente Cryptosporidium galli. Embora em todos os criatórios houvesse aves positivas para C. galli, a presença de morbidade ou mortalidade foi observada em aves de somente um criatório, e estava associada à infecção concomitante com Escherichia coli e Isospora spp..Este é o primeiro relato de infecção por C. galli em P. brissonii, O. maximiliani e S. collaris
Due to the lack of information related to the epidemiology of Cryptosporidium infection in passerine birds, this study aimed to determine the frequency of fecal shedding of Cryptosporidium spp. oocysts, after natural infection, the clinical signs and the presence of mortality, and accomplish its molecular characterization. Four hundred and eighty fecal samples were collected from 40 birds, 372 samples from 31 adult birds and 108 samples from young birds (up to 12 months old), housed in five herds, monthly, from September 2007 to September 2008, with the exception of the April. The birds were originated from flocks were the following species were herd: great-billed seed-finch (Oryzoborus maximiliani), lesser seed-finch (Oryzoborus angolensis), ultramarine grosbeak (Passerina brissonii) and rusty-collared seedeater (Sporophila collaris). The samples were preserved in 2.5% potassium dichromate 2.5% at 4°C, until processing. The oocysts were purified by centrifugal flotation in Sheather solution, followed by genomic DNA extraction from oocysts and molecular characterization using the nested polymerase chain reaction for amplification of fragments of the 18S subunit ribosomal RNA gene. Intermittent fecal shedding of Cryptosporidium spp. was observed in 91 (24.5%) samples from adult birds, with more frequent in periods approaching the periods of moulting and reproduction of birds and 14 samples (13%) of young birds.The sequencing of the amplified fragments allowed the identification of Cryptosporidium galli. Although all the aviaries had birds positive for C. galli, morbidity or mortality was observed in birds from only one aviary, and was associated to concomitant infection with Escherichia coli and Isospora sp. This is the first report of infection by C. galli in Oryzoborus maximiliani, Passerina brissonii, and Sporophila collaris
Silva, Deuvânia Carvalho da. "Avaliação física, epidemiológica e molecular da infecção por Cryptosporidium spp. em passeriformes /". Araçatuba : [s.n.], 2009. http://hdl.handle.net/11449/94711.
Testo completoBanca: Valéria Marçal Félix de Lima
Banca: Rodrigo Martins Soares
Resumo: Devido à carência de informações relacionadas à epidemiologia da infecção por Cryptosporidium spp. em passeriformes, neste trabalho objetivou-se determinar a periodicidade da eliminação fecal de oocistos de Cryptosporidium, os sinais clínicos, a presença de mortalidade, e a caracterização molecular desse coccídio. Foram colhidas 480 amostras de fezes, provenientes de 40 aves, sendo 372 amostras de 31 aves adultas e 108 amostras de nove filhotes até 12 semanas de vida, com periodicidade mensal, no período de setembro de 2007 a setembro de 2008, com exceção do mês de abril. As aves estavam alojadas em cinco criatórios, com criação de bicudo (Oryzoborus maximiliani), curió (Oryzoborus angolensis), azulão (Passerina brissonii) e coleira do brejo (Sporophila collaris). As amostras foram conservadas em bicromato de potássio 2,5%, a 4ºC, até o processamento. Os oocistos foram purificados por centrífugo-flutuação em solução de Sheather, seguindo-se a extração do DNA genômico dos oocistos e a classificação molecular, por meio da reação em cadeia de polimerase-nested, para amplificação de fragmentos da subunidade 18S do gene do RNA ribossômico. Eliminação fecal intermitente de Cryptosporidium spp. foi observada em 91 (24,5%) amostras de aves adultas, com maior ocorrência nos períodos que se aproximam dos períodos de muda de penas e de reprodução das aves e em 14 amostras (13%) de aves jovens. O sequenciamento dos fragmentos de DNA amplificados possibilitou a identificação de somente Cryptosporidium galli. Embora em todos os criatórios houvesse aves positivas para C. galli, a presença de morbidade ou mortalidade foi observada em aves de somente um criatório, e estava associada à infecção concomitante com Escherichia coli e Isospora spp..Este é o primeiro relato de infecção por C. galli em P. brissonii, O. maximiliani e S. collaris
Abstract: Due to the lack of information related to the epidemiology of Cryptosporidium infection in passerine birds, this study aimed to determine the frequency of fecal shedding of Cryptosporidium spp. oocysts, after natural infection, the clinical signs and the presence of mortality, and accomplish its molecular characterization. Four hundred and eighty fecal samples were collected from 40 birds, 372 samples from 31 adult birds and 108 samples from young birds (up to 12 months old), housed in five herds, monthly, from September 2007 to September 2008, with the exception of the April. The birds were originated from flocks were the following species were herd: great-billed seed-finch (Oryzoborus maximiliani), lesser seed-finch (Oryzoborus angolensis), ultramarine grosbeak (Passerina brissonii) and rusty-collared seedeater (Sporophila collaris). The samples were preserved in 2.5% potassium dichromate 2.5% at 4°C, until processing. The oocysts were purified by centrifugal flotation in Sheather solution, followed by genomic DNA extraction from oocysts and molecular characterization using the nested polymerase chain reaction for amplification of fragments of the 18S subunit ribosomal RNA gene. Intermittent fecal shedding of Cryptosporidium spp. was observed in 91 (24.5%) samples from adult birds, with more frequent in periods approaching the periods of moulting and reproduction of birds and 14 samples (13%) of young birds.The sequencing of the amplified fragments allowed the identification of Cryptosporidium galli. Although all the aviaries had birds positive for C. galli, morbidity or mortality was observed in birds from only one aviary, and was associated to concomitant infection with Escherichia coli and Isospora sp. This is the first report of infection by C. galli in Oryzoborus maximiliani, Passerina brissonii, and Sporophila collaris
Mestre
Miller, Woutrina Ann. "Cryptosporidium species in coastal California ecosystems /". For electronic version search Digital dissertations database. Restricted to UC campuses. Access is free to UC campus dissertations, 2004. http://uclibs.org/PID/11984.
Testo completoGhaffari, Salman. "A genotyping study of Cryptosporidium species". Thesis, University of Liverpool, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.425447.
Testo completoEdwards, Hanna. "The 100 Faces of Cryptosporidium parvum". Thesis, Edwards, Hanna (2012) The 100 Faces of Cryptosporidium parvum. PhD thesis, Murdoch University, 2012. https://researchrepository.murdoch.edu.au/id/eprint/10792/.
Testo completoSilva, Sheila Oliveira de Souza. "Padronização de uma reação em cadeia pela polimerase em nested (nested-PCR) para detecção e diferenciação das espécies de Cryptosporidium spp e caracterização molecular de Cryptosporidium isolados de roedores sinantrópicos". Universidade de São Paulo, 2011. http://www.teses.usp.br/teses/disponiveis/10/10134/tde-24042012-151514/.
Testo completoCryptosporidium spp are cosmopolitan protozoan that infect fish, reptiles, amphibians, birds and mammals. More than 20 species are recognized within this genus. Rodents are groups of abundant and ubiquitous organisms that have been considered reservoirs of Cryptosporidium for infection of humans and livestock. The coding sequences of the smallest unit ribosomal (18S rRNA) of Cryptosporidium spp are characterized by intercalations amongst polymorphic and conserved regions along its 1700 base pairs. The aim of this study was to design primers specific for the 18S rRNA gene, potentially capable of amplifying any species or genotype of Cryptosporidium spp., and evaluate the attributes of the nested-PCR diagnosis based on such probes. The design of primers was performed to amplify a smaller segment as possible to maximize the sensitivity of molecular testing and preserving the discriminatory potential of the sequences amplified. The nested-PCR standardized in this study (nPCR-SH) was compared with another similar assay that has been widely used for detection and identification of Cryptosporidium spp. worldwide (nPCR-XIAO). It also aimed to characterize molecularly Cryptosporidum spp. isolated from synanthropic rodents, using these probes and targeted molecular probes. Forty five rodents were captured in public areas of the urban area of Umuarama, Paraná. The samples were subjected to three molecular tests, two targeted to gene18S rRNA (nPCR-SH and nPCR-XIAO) and another targeted to the gene encoding actin. The nPCR-SH was tested with strains of Cryptosporidum parvum, Cryptosporidum andersoni, Cryptosporidum meleagridis, Cryptosporidum hominis Cryptosporidum canis, Cryptosporidum serpentis and all were positive. Sixteen samples of rodents were positive by nPCR-SH, six by nPCR-XIAO and five by nPCR targeted to the gene encoding actin. The sequencing of the amplified fragments allowed the identification of Cryptosporidum Muris in three samples of Rattus rattus, and two new genotypes of Cryptosporidium rat genotype II and III. It was found rat genotype II in a sample of Mus musculus and genotype III in twelve samples, five from Rattus rattus and seven from Mus Musculus.The results showed that the primers designed for detection of Cryptosporidium spp in fecal samples were more efficient in amplifying regions that allow the distinction amongst the parasite species than those used in the PCR-XIAO. Cryptosporidium species or genotypes transmitted to other species such as zoonotic were not found in the studied samples, which suggest that the importance of these animals in the zoonotic transmission of cryptosporidiosis is very limited.
Drozd, Céline. "Comportement de Cryptosporidium spp. Dans l'eau : conséquences au niveau de la microfiltration tangentielle". Nancy 1, 1996. http://docnum.univ-lorraine.fr/public/SCD_T_1996_0386_DROZD.pdf.
Testo completoCelis, Samanez Noemit Norma. "Criptosporidiasis en caninos críados en comunidades campesinas de tres distritos del departamento de Puno". Bachelor's thesis, Universidad Nacional Mayor de San Marcos, 2010. https://hdl.handle.net/20.500.12672/696.
Testo completo-- The objective of this study was to estimate the prevalence of Cryptosporidium sp. in dogs of rural communities, located in the districts of Ajoyani; province Carabaya; Palca and Santa Lucia; province Lampa, Puno. Were collected 123 fecal samples from dogs apparently healthy, of both sexes and different ages, which were between 1 month and 16 during the months of February and March 2009. Feces were transported to the Laboratory of INIA Quimsachata (Puno) where are the faecal frotices being fixed in methanol. Subsequently were transported to the Parasitology Laboratory of the FMV-Lima, for diagnosis; which was performed using the Ziehl-Neelsen modified. The overall prevalence of Cryptosporidium sp. was 26.8±7.8%, were found prevalences of 19.0, 28.6 and 28.4% in the districts of Ajoyani, Palca, and St. Lucia, respectively, males and females, showed prevalences of 28.3 and 17.6% respectively and according to age groups 0 -6,> 6-12,> 12-72 and > 72 months were 46.2, 31.3, 19.7, 29.4%, respectively. Was applied Chi-square test with a significance level of 0.05. Statistical analysis showed no significant association (p> 0.05) between this protozoan of domestic dogs with the district, sex and age. Keywords: Cryptosporidium sp, protozoa, zoonoses, prevalence, dogs.
Tesis
Li, Hanbin. "Chemical inactivation of Cryptosporidium parvum in water". Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2001. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp04/NQ60318.pdf.
Testo completoBouzid, Maha. "Postgenomics analyses of species-specific Cryptosporidium genes". Thesis, University of East Anglia, 2010. https://ueaeprints.uea.ac.uk/19907/.
Testo completoBarakat, Farah Mukhlis. "Protective innate immune responses against Cryptosporidium parvum". Thesis, Queen Mary, University of London, 2013. http://qmro.qmul.ac.uk/xmlui/handle/123456789/8733.
Testo completoMiller, Christopher. "Establishing Cryptosporidium parvum as a model organism". Thesis, University of Kent, 2017. https://kar.kent.ac.uk/66710/.
Testo completoLally, Nicola C. "Antigen encoding gene fragments of Cryptosporidium parvum". Thesis, University of Edinburgh, 1992. http://hdl.handle.net/1842/11026.
Testo completoPalermo, Cindy. "Cryptosporidium in fish: Morphological and molecular characterisation". Thesis, Palermo, Cindy (2016) Cryptosporidium in fish: Morphological and molecular characterisation. Honours thesis, Murdoch University, 2016. https://researchrepository.murdoch.edu.au/id/eprint/35248/.
Testo completoNg, Josephine Su Yin. "Molecular epidemiology and metabolomic characterisation of Cryptosporidium". Thesis, Ng, Josephine Su Yin (2017) Molecular epidemiology and metabolomic characterisation of Cryptosporidium. PhD thesis, Murdoch University, 2017. https://researchrepository.murdoch.edu.au/id/eprint/38641/.
Testo completoBolland, Samuel John. "Describing new species of Cryptosporidium in fish". Thesis, Bolland, Samuel John (2019) Describing new species of Cryptosporidium in fish. Honours thesis, Murdoch University, 2019. https://researchrepository.murdoch.edu.au/id/eprint/54961/.
Testo completoSargent, Keith. "Molecular characterization of Cryptosporidium from selected hosts". Thesis, Sargent, Keith (1997) Molecular characterization of Cryptosporidium from selected hosts. Honours thesis, Murdoch University, 1997. https://researchrepository.murdoch.edu.au/id/eprint/58326/.
Testo completoMychajlonka, Meisha Natasha. "Worldwide Distribution of Cryptosporidium Species in Bovines". Thesis, The University of Arizona, 2015. http://hdl.handle.net/10150/579293.
Testo completoCifrino, Andrew Charles 1955. "Isolation and concentration of Cryptosporidium from water". Thesis, The University of Arizona, 1986. http://hdl.handle.net/10150/191904.
Testo completoSturbaum, Gregory Dean. "Cryptosporidium parvum, molecular environmental detection and implications". Diss., The University of Arizona, 2003. http://hdl.handle.net/10150/280454.
Testo completoBaishanbo, Asiya. "La cryptosporidiose : modèles expérimentaux d'infection par Cryptosporidium hominis et Cryptosporidium parvum génotypes 2 et d'hypersensibilité viscérale post-infectieuse. Applications pharmacologiques". Rouen, 2005. http://www.theses.fr/2005ROUE01NR.
Testo completoCryptosporidium sp. Are emergent pathogenic protozoan parasites now recognised as one of the most common causes of human enteric infections. In man, the most prevalent genotypes are Cryptosporidium hominis (previously Cryptosporidium parvum type 1) and the bovine C. Parvum genotype 2. In spite of recent advances, the pathogenesis of cryptosporidiosis is presently poorly understood and a limited number of effective anticryptosporidial therapeutic agent are available. Our objective was to develop Cryptosporidium sp. Infection models in immunocompetent rats and immunosuppressed gerbil to assess infectivity and pathogenicity, and to screen anticryptosporidial agents. C. Hominis and C. Parvum genotype 2 oocyts were found sustainably infective for immunosuppressed Mongolian gerbils at doses ranging from 100 to 200 000 oocysts/ animal for both isolates. Moreover, this model was found suitable to study both ileal and biliary cryptosporodial agents. Although it did not achieve complete removal of Cryptosporidium sp. From the infected host, nitazoxanide was the only pharmacological agent effective againts gall bladder infection. The in vitro activity of 52 flavonoids was studied against the Apicomplexan pathogenic parasites Cryptosporidium parvum, Sarcocystis neurona and Neospora caninum. Twenty compounds were found able to significantlly and dose dependently inhibit in vitro parasite development and exhibited more than 95% inhibition against one or, for 8 agents, the three coccidian parasites. In the immunosuppressed gerbil model, 2 agents (RM 6427 and RM 6428) were found efficient not only in decreasing oocyst shedding but also in limiting C. Parvum intracellular developpment in the gut and in the biliary tract. These activities were significantly higher than those of nitazoxanide and paromomycin. In humans, another concern in the physiopathology of cryptosporidiosis is its possible role in the development of post infectious irritable bowel disease. In an immunocompetent suckling rat model, an increases sensitivity to jejunal distension at any distending volume compared to uninfected control rats was obserced 120 days after recovery of Cryptosporidium parvum infection. In accordance with clinicam studies in man, present results in rats prompt investigation of the long-term role of enteric Cryptospordium parvum infection in the pathogenesis of irritable bowel disease
Oliveira, Paula Vilma de. "Ocorrencia de cistos de Giardia spp. e oocistos de Cryptosporidium spp. no rio Atibaia, bacia do rio Piracicaba, Campinas, São Paulo". [s.n.], 2005. http://repositorio.unicamp.br/jspui/handle/REPOSIP/313872.
Testo completoDissertação (mestrado) - Universidade Estadual de Campinas. Instituto de Biologia
Made available in DSpace on 2018-08-04T03:37:16Z (GMT). No. of bitstreams: 1 Oliveira_PaulaVilmade_M.pdf: 3163607 bytes, checksum: cf552ae60ce22ed636ee5cbfa344ebd8 (MD5) Previous issue date: 2005
Resumo: Giardia spp. e Cryptosporidium spp. estão amplamente dispersos no ambiente aquático e foram responsáveis por diversos surtos de giardiose e criptosporidiose por veiculação hídrica em vários países. A presença destes patógenos em água utilizada para consumo humano é um fator de risco para aquisição destas parasitoses porque cistos e oocistos são resistentes aos processos de tratamento e às condições ambientais. Deste modo, a preocupação em relação à contaminação das fontes de água para uso humano por estes protozoários é crescente. No Brasil, há registros da ocorrência de ambos organismos em água superficial e subterrânea e em esgoto bruto e tratado. Atualmente, há uma recomendação oficial para a implantação da pesquisa de cistos e oocistos em água tratada. Assim, é necessário ter conhecimento sobre a freqüência e a distribuição destes parasitos nos mananciais. Os objetivos do presente estudo foram: i) verificar a ocorrência de cistos de Giardia e oocistos de Cryptosporidium em amostras de água bruta superficial do Rio Atibaia (Campinas, São Paulo) utilizando a técnica de filtração em membrana (47 mm diâmetro; 3 ?m de porosidade nominal); ii) avaliar a influência do desaguamento do Ribeirão Pinheiros na qualidade da água do Rio Atibaia mediante a comparação e a correlação entre parâmetros microbiológicos, físicos, pluviométricos e concentração de cistos e oocistos em dois pontos: à montante (A1) e à jusante (A2) do desaguamento do Ribeirão Pinheiros; e iii) verificar a ocorrência de variação sazonal destes protozoários na água superficial do Rio Atibaia. De março de 2002 a abril de 2003, 50 amostras de água foram analisadas. No ponto A1, cistos de Giardia foram detectados em 52,0% das amostras (concentração = 25,12 cistos/l), enquanto no ponto A2, a positividade foi de 76,0% (64,16 cistos/l). Oocistos de Cryptosporidium foram detectados somente no ponto A2, em 0,04% das amostras (0,8 oocistos/l). Houve diferença significativa na concentração de cistos entre os pontos A1 e A2 (p = 0,011) e correlação entre a pluviosidade e os parâmetros: concentração de cistos de Giardia (r = 0,611); coliformes totais (r = 0,467) e fecais (r = 0,467) no ponto A2. Em ambos os pontos, não foi encontrada correlação entre concentração de cistos de Giardia e as variáveis: coliformes totais (A1: r = -0,276; A2; r = -0,201), fecais (A1: r = -0,187; A2: r = -0,201) e turbidez (A1: r = -0,252; A2: r = -0,055). Dentre estes parâmetros, apenas a turbidez teve variação entre verão/inverno e verão/outono (p = 0,06). A técnica de filtração em membrana apresentou uma eficiência de recuperação de 72,2% para cistos de Giardia e de 65,1% para oocistos de Cryptosporidium. Os resultados obtidos neste estudo são relevantes porque o Rio Atibaia é o manancial que abastece a cidade de Campinas e outros municípios da região e o conhecimento da epidemiologia ambiental destes protozoários é importante do ponto de vista da Saúde Pública e também das companhias de tratamento de água
Abstract: Giardia spp. and Cryptosporidium spp. are widely dispersed in the aquatic environment and are responsible for several waterborne outbreaks of giardiosis and cryptosporidiosis in many countries. The occurrence of these pathogens in drinking water is a risk factor for human infection because of the resistance of cyst and oocyst to water treatment processes and to the environment. Nowadays, there is an increasing concern about drinking water contamination by these protozoans. In Brazil, the presence of both organisms was registered in groundwater and superficial water and in raw and treated sewage. Currently, there is an official recommendation for monitoring the presence of Giardia and Cryptosporidium in drinking water samples; thus a federal decree aims to establish researches for (oo)cysts detection in treated water. The knowledge about the frequency and distribution of these protozoan parasites in water sources is necessary to establish policies on public health. The aims of the present study are: i) to verify the occurrence of Giardia cysts and Cryptosporidium oocysts in Atibaia River¿s (Campinas City, São Paulo State) superficial raw water samples using a membrane filtration method (47 mm diameter; 3 ?m of nominal porosity; ii) to evaluate the influence of the discharge of Pinheiros Stream on the water quality of the Atibaia River by the correlationship among microbiological and physical parameters, rainfall and oocysts levels in two points: upstream (A1) and downstream (A2) of Pinheiros Stream; and iii) to verify the seasonal variation of these protozoa occurrence in the superficial water of Atibaia River. From March 2002 to April 2003, 50 water samples were collected. In point A1, Giardia cysts were detected in 52,0% of the samples (concentration = 25,12 cysts/l) while in point A2 the positivity for this parasite was 76,0% (concentration = 64,16 cysts/l). Cryptosporidium oocysts were detected only in A2 point, in 0,04% of the samples (concentration = 0,8 oocysts/l). There was a significant difference for cysts concentration between A1 and A2 points (p = 0,011) and among rainfall and the parameters: cyst concentration (r = 0,611); total (r = 0,467) and fecal coliforms (r = 0,467) in A2 point. In both points, it was not found a correlationship among cyst concentration and the following parameters: total coliforms (A1: r = -0,276; A2; r = -0,201), fecal coliforms (A1: r = -0,187; A2: r = -0,201) and turbidity (A1: r = -0,252; A2: r = -0,055). The turbidity showed a variation between summer and winter periods as well as summer and fall periods (p = 0,06). The membrane filtration technique attained recovery efficiency rates of 72,2% for Giardia cysts and 65,1% for Cryptosporidium oocysts. The data obtained in this study are relevant because the Atibaia River is the major water source found in Campinas and other cities in the region. Thus, the knowledge about the environmental epidemiology of these protozoa is of very important to policies on to the Public Health and to the drinking water industry
Mestrado
Parasitologia
Mestre em Parasitologia
França, Rita Borges. "Cryptosporidium spp., Giardia spp. e ovos de helmintos em esgoto hospitalar : destruição e analise de dano estrutural dos protozoarios apos o processo fotoeletroquimico". [s.n.], 2007. http://repositorio.unicamp.br/jspui/handle/REPOSIP/315635.
Testo completoDissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Biologia
Made available in DSpace on 2018-08-09T07:00:20Z (GMT). No. of bitstreams: 1 Franca_RitaBorges_M.pdf: 4952448 bytes, checksum: 49473f55f00ae94646a96bb8c6672aac (MD5) Previous issue date: 2007
Resumo: O efluente hospitalar apresenta, dentre seus componentes, organismos como vírus, bactérias, protozoários e helmintos, que ocasionam muitas doenças com implicações em saúde pública. Cryptosporidium spp. e Giardia spp. são protozoários parasitos com grande importância por sua veiculação hídrica e cujas formas infectantes são resistentes aos processos rotineiramente usados no tratamento de água e esgoto. A transmissão destes pode ocorrer com a ingestão dos oocistos e cistos eventualmente presentes na água e nos alimentos contaminados, por contato direto (pessoa a pessoa), por contato indireto (objetos contaminados), pelo contato sexual ou pode ser zoonótica. Os métodos mais utilizados para desinfecção em estações de tratamento são a aeração, cloração e irradiação por UV, mas a cloração, não é suficiente para eliminar oocistos de Cryptosporidium spp e cistos de Giardia spp. A tecnologia eletroquímica oferece um meio de tratamento eficiente para a oxidação. da carga orgânica e microbiológica degradando-as ou mineralizando-as. O presente trabalho teve por objetivos: (1) verificar a ocorrência natural de Cryptosporidium spp. e Giardia spp. em amostras de esgoto do Hospital de Clínicas de Campinas, utilizando o método, de centrífugo-concentração seguido de clarificação com éter e visualização por, imunofluorescência direta, durante o período de um ano; (2) verificar a presença de ovos e larvas de helmintos no esgoto hospitalar empregando a técnica da NOM (Norma Oficial Mexicana) e (3) avaliar a taxa de destruição e o dano estrutural causado em cistos e oocistos após o tratamento fotoeletroquímico. No esgoto hospitalar bruto 4,1 % e 58,3 % das amostras foram positivas para Cryptosporidium spp. e Giardia spp., respectivamente, sendo observada a concentração média de 2,7 x 103 oocistos/L e 3,8 x 105 cistos/L. Foi possível verificar a elevada presença de helmintos, com 90 % das amostras apresentando positividade e concentração de 5,8 x 104 ovos/L e 4,0 x 105 larvas/L. Os protozoários e helmintos presentes em altas concentrações no esgoto hospitalar representam uma séria ameaça à saúde humana. Para os ensaios com o tratamento fotoeletroquímico, amostras de 1 L de esgoto hospitalar foram artificialmente contaminadas com cistos e oocistos e, posteriormente, submetidas a esse tratamento em um reator de bancada, com tempos de exposição de 0,30, 60 e 90 minutos. Por meio das técnicas de imunofluorescência direta, microscopia de contraste de fase e microscopia eletrônica de varredura verificou-se o dano estrutural causado pela ação dos radicais hidroxila nesses protozoários patogênicos e a destruição dos mesmos. O tratamento fotoeletroquímico mostrou uma redução na concentração dos protozoários nos tempos de 30 e 60 minutos e após 90 minutos, nenhum cisto ou oocisto foi detectado. A presença do cloreto no efluente bruto (média de 45 mg/l) desencadeou uma potencializaçáo da ação de mecanismo do reator, gerando efeito associado com a eletrólise, dos radicais hidroxila com a formação de hipoclorito
Abstract: Hospital effluent presents organisms as virus, bacteria, protozoan and helminthes, that cause many iIInesses with implications in public health. Cryptosporidium spp. and Giardia spp. are parasites with waterborne importance and its cysts and oocysts are resistant to the routinely processes used in water treatment. Their transmission can occur by oocysts and cysts ingestion in the water and contaminated foods, by direct contact (person the person), by indirect contact (contaminated objects), by sexual contact or zoonotic. The methods used for disinfection and treatment of sewage are aeration, chlorination and irradiation of ultraviolet light, but the treatment by chlorination is not enough to inactivate Cryptosporidium spp. oocyst and Giardia spp. cysts. The electrochemical technology offers an efficient treatment for the oxidation of organic and microbiological load, degrading and mineralizing them. The present work had as objectives: (1) to verify the natural occurrence of Cryptosporidium spp. and Giardia spp. in samples of Clinical Hospital sewage from Unicamp using centrifugal concentration followed by clarification with ether method and visualization by immunoflourescence assay, during one year, (2) to verify the presence of eggs and larvae of helminthes in the hospital sewage by NOM (Mexican Official Norm) technique and (3) to evaluate the destruction rate and the structural damage caused in cysts and oocysts by photoelectrochemical treatment. In raw hospital sewage 4.1 % and 58.3% of the samples were positive for Cryptosporidium spp. and Giardia spp., respectively, with concentrations of 2.7 x 103 oocysts/L and 3.8 x 105 cysts/L. The high presence of helminthes, 90% positive, with 5.8 x 104 eggs/L and 4.0 x 105 larvae/L and protozoan in hospital sewage represent a serious threat to human being health. For the assays with the photoelectrochemical treatment, samples of 1 L of hospital sewage artificially contaminated with cysts and oocysts ! were submitted to this treatment in a bench reactor, with times of exposition of 0, 30, 60 and 90 minutes. By the techniques of immunofluorescence assays, microscopy of phase contrast and scanning electronic microscopy, the structural damage and destruction were observed, caused by hydroxyl radicals in these pathogenic protozoans. The photoelectrochemical treatment showed a concentration reduction of the protozoan in 30 and 60 minutes, and after 90 minutes no cyst or oocysts were detected. The chloride present in raw effluent (average of 45 rng/L) unchained a potential action of the reactor mechanism, generating an effect associated with electrolysis of the hydroxyl radicals with production of hypochlorite
Mestrado
Parasitologia
Mestre em Parasitologia
Coutinho, Tatiane Sueli. "Avaliação do efeito de microrganismos probióticos sobre Cryptosporidium parvum em camundongos C57BL/6 imunossuprimidos". Universidade de São Paulo, 2008. http://www.teses.usp.br/teses/disponiveis/97/97131/tde-20082013-164015/.
Testo completoThe probiotics are products or preparation containing viable microorganisms, in defined concentrations, capable of change the host\'s microbiota, exerting beneficial effects on the host\'s health. The most important microorganisms which present these properties belong to the group of lactic acid bacterium, mainly species of Lactobacillus. Even though technological advances in this area, few studies are found concerning the action probiotics on protozoan, as Cryptosporidium parvum, an important intestinal parasite which causes one of the main opportunist infection in immunodeficient individuals, such as AIDS and transplanted patients. The probiotics products can be an alternative to treatment against cryptosporidiosis, since, so far an effective treatment against this infection has not been found. Therefore, the present study aimed to evaluate the action of strains of Lactobacillus plantarum, L. acidophillus and L. delbruekii, as a pool, on Cryptosporidium parvum in immunosuppressed mice. The experiments were developed using 42 animals separated in six groups: Control - the mices were not immunosuppressed, no infected and no treated; Immuno - immunosuppressed, no infected and no treated; No treated - immunosuppressed, infected and no treated; NTZ - immunosuppressed, infected and treated with a Nitazoxanide antimicrobial (150mg/kg); PRO - immunosuppressed, infected and treated with a preparation probiotic (3x10¹²UFC/mL); and, Preventive - immunosuppressed, treated with the probiotic preparation and then infected. After 12 days of immunosuppression procedure the animals were infected with 1,6x106 oocysts and, the respective treatments were started after five days of the inoculation of the oocysts. The infection was analyzed by counting of purified oocysts by tecnic of formol ether sedimentation of the feces. The intestinal colonization by lactobacilli was analyzed by pour plate technique on MRS agar containing Rifampicin antibiotic. The nutritional performance of the animals was calculated based on the rate of feed conversion, which is a relation between the amount of food consumed and the body weight gained by each animal. The results showed that the animals that received a preventive treatment with the probiotic preparation presented an elimination of oocysts lower than (P<=0,05) the other infected groups. The treatment with the probiotic preparation, post-infection also showed to be effective, once all the animals eliminated the infection. These results can be explained by competitive exclusion due to the colonization of gastrointestinal tract of the animals or by production of antimicrobial substances produced by Lactobacillus, which presented somehow any action on oocysts. It was also observed that the treated animals with the probiotic preparation presented better nutritional performance. These results confirm the hypothesis that the microorganisms which present probiotic properties represent an alternative way and promising, in the preventing and treatment of cryptosporidiosis.
Gyürék, Lyndon Lester. "Ozone and chlorine inactivation of Cryptosporidium in water". Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp04/nq22990.pdf.
Testo completoEmelko, Monica Beata. "Removal of Cryptosporidium parvum by granular media filtration". Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2001. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp04/NQ60533.pdf.
Testo completoAli, Sayma. "The development of molecular methods for Cryptosporidium epidemiology". Thesis, Coventry University, 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.418355.
Testo completoNichols, Gordon L. "The biology, epidemiology and typing of Cryptosporidium spp". Thesis, University of Surrey, 1992. http://epubs.surrey.ac.uk/2235/.
Testo completoPugh, Hedley James. "Deposition and adhesion of cryptosporidium oocysts on surfaces". Thesis, University College London (University of London), 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.300120.
Testo completoStenger, Brianna Leigh Schneck. "Ecology of Cryptosporidium Parasites in Wild Rodent Populations". Diss., North Dakota State University, 2015. https://hdl.handle.net/10365/27278.
Testo completoUSDA (project number: 2008-35102-19260)
NIH (project numbers: 2P20 RR015566, 1R15A1067284-01A1)
Tabe, Ebot Sahidu. "Rhomboid Proteases and Surface Adhesins During Cryptosporidium Development". Diss., North Dakota State University, 2013. https://hdl.handle.net/10365/26889.
Testo completoKoh, Wan Hon. "The interaction of cryptosporidium with aquatic biofilm systems". Thesis, Koh, Wan Hon (2013) The interaction of cryptosporidium with aquatic biofilm systems. PhD thesis, Murdoch University, 2013. https://researchrepository.murdoch.edu.au/id/eprint/20253/.
Testo completoKorich, Dick Gary. "Cryptosporidium oocyst viability: Assessment and correlation with infectivity". Diss., The University of Arizona, 1993. http://hdl.handle.net/10150/186125.
Testo completoSullivan-Madore, Mary 1959. "Detection of Cryptosporidium and Giardia in environmental waters". Thesis, The University of Arizona, 1986. http://hdl.handle.net/10150/191916.
Testo completoHomem, Camila Guariz [UNESP]. "Avaliação da PCR em tempo real para detecção de Cryptosporidium parvum em amostras fecais de bezerros: Camila Guariz Homem. -". Universidade Estadual Paulista (UNESP), 2011. http://hdl.handle.net/11449/94592.
Testo completoFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
A infecção por Cryptosporidium parvum em bovinos se manifesta como enfermidade subclínica ou com presença de morbidade e mortalidade, particularmente quando há associação com outros agentes infecciosos. Cryptosporidium parvum apresenta ainda grande importância em saúde pública. Este trabalho teve como objetivo o desenvolvimento da reação em cadeia da polimerase em tempo real (qPCR) para detecção de C. parvum em amostras fecais de bezerros e sua comparação com a reação em cadeia da polimerase (nested PCR), rotineiramente utilizada para diagnóstico de Cryptosporidium spp.. Duzentas e nove amostras fecais de bezerros entre um dia e seis meses de idade foram examinadas pela qPCR para amplificação de fragmentos do gene da actina e pela nested PCR para o gene da subunidade 18S do rRNA. A qPCR apresentou positividade para C. parvum em 73,21% (153/209) das amostras, enquanto a nested PCR apresentou amplificação positiva para Cryptosporidium spp. em 56,5% (118/209) das amostras. A sensibilidade analítica da qPCR foi de aproximadamente um oocisto de C. parvum. Não se observou amplificação inespecífica de DNA Cryptosporidium bovis, Cryptosporidium andersoni, Cryptosporidium ryanae, Cryptosporidium canis, Cryptosporidium serpentis, Cryptosporidium galli, Cryptosporidium baileyi e Cryptosporidium genótipo II de aves. Desta forma, conclui-se que a qPCR para o gene da actina é uma técnica sensível e específica para detecção de C. parvum em amostras fecais de bezerros
The infection with Cryptosporidium parvum in cattle results in subclinical disease or in the presence of morbidity and mortality, particularly when associated with other infectious agents. This species is also an important public health problem. The aim of this research was to develop a real time polymerase chain reaction (qPCR) for detection of C. parvum DNA in fecal samples of calves, in comparison to a nested PCR routinely used for Cryptosporidium spp. diagnosis. Two hundred and nine fecal samples from calves aged between one day and six weeks were screened by qPCR specific for the actin gene of C. parvum and using nested PCR targeting the 18S rRNA gene of Cryptosporidium spp.. The qPCR showed positivity for C. parvum in 73,21% (153/209) of the samples, and nested PCR was positive for Cryptosporidium spp. in 56.5% (118/209) of the samples. The analytical sensitivity of qPCR foi de aproximadamente one oocyst C. parvum per reaction tube. Evaluation of analytical specificity did not reveal inespecific amplification for DNA of the following Cryptosporidium species and genotypes: C. bovis, C. andersoni, C. ryanae, C. canis, C. serpentis, C. galli, C. baileyi and avian genotype II. These results allowed the conclusion that qPCR for actin gene is a sensitive and specific technique for detection of C. parvum in fecal samples from calves
Santos, Samuel Ricardo dos 1980. "Fluorescência retardada em protozoários : Giardia intestinalis e Cryptosporidium parvum". [s.n.], 2014. http://repositorio.unicamp.br/jspui/handle/REPOSIP/257131.
Testo completoTese (doutorado) - Universidade Estadual de Campinas, Faculdade de Engenharia Agrícola
Made available in DSpace on 2018-08-25T21:52:58Z (GMT). No. of bitstreams: 1 Santos_SamuelRicardodos_D.pdf: 51294263 bytes, checksum: 55a8bb93513a62aa29e85fe9467412c6 (MD5) Previous issue date: 2014
Resumo: Giardia spp. e Cryptosporidium spp. são organismos desafiadores em monitoramento ambiental, podendo afetar os seres humanos e os animais com grandes impactos na saúde pública. Métodos para detectar esses organismos são descritos na literatura ¿ p.ex.: o método EPA 1623.1. No entanto, muitos não são capazes de detectar a viabilidade destes parasitos. Este trabalho avaliou o uso de marcadores fluorescentes combinados com a técnica de detecção de fluorescência retardada na detecção de viabilidade de Giardia intestinalis e Cryptosporidium parvum. Testes de incubação com 6-carboxifluorceina-succinimidil-diacetato-éster (CFDA-SE), C12-resazurina e SYTOX Green foram desenvolvidos com cistos de G. intestinalis e oocistos de C. parvum. Medidas de fluorescência retardada em câmara escura projetada e em dispositivo comercial foram aplicados em amostras purificadas e concentradas de G. intestinalis após incubação com CFDA-SE. Grupos contendo cistos vivos e infecciosos, mortos a 100° C, estressados com luz UV-C e envelhecidos foram analisados via fluorescência retardada e microscopia de epifluorescência em oito séries experimentais. Os resultados demonstram que (oo)cistos vivos e infecciosos não são marcados com os marcadores fluorescentes. Dupla marcação em (oo)cistos mortos é observada após 30 minutos de incubação com C12-resazurina 5,0 ?M e SYTOX Green 100 nM. (Oo)cistos mortos apresentam marcação verde após incubação de CFDA-SE 5,0 ?M. O envelhecimento da amostra foi acompanhado pelo aumento da taxa de marcação celular com cistos apresentando ~50% de marcação aos 30 dias de idade e ~100% aos 50 dias de idade. Testes com fluorescência retardada demonstram que cistos vivos e com idade inferior a 20 dias apresentam intensidades superiores aos cistos mortos e estressados após excitação com 365 nm. A excitação com 365 nm apresentou correlação R2 > 95% após análise de cinética de decaimento com modelo exponencial de segunda ordem. Os dados indicam que o decaimento da fluorescência retardada é acompanhado por duas componentes k1 e k2 onde k2 = 5?k1, estando estas conectadas com as condições fisiológicas da Giardia. O procedimento pode ser efetuado em 10 passos laboratoriais em aproximadamente 60 minutos de análise. A fluorescência retardada apresenta futuro promissor na análise de viabilidade de parasitos em amostras purificadas
Abstract: Giardia spp. and Cryptosporidium spp. are challenging and important organisms in modern environmental monitoring. These protozoa can affect humans and animals seriously, as reflection of sanitation problems in water quality control, with huge impact over economics and public health. Methods to detect such organisms are well described in literature - i.e. the EPA Method 1623.1 and AWWA 2012. But those ones are not able to detect infectivity. For that, the usual procedures include infection of animal model leading to at least one week for confirming infectivity. Some research with dye probes are being developed in order to provide useful, reliable and low cost procedures for detection of protozoa viability, i.e. enabling to distinguish dead samples cells from living ones. This work describes the screening tests for viability detection of protozoa samples - Giardia intestinalis and Cryptosporidium parvum - using carboxifluorcein-succinimidyl-diacetate-ester (CFDA-SE), C12-resazurin and SYTOX Green. Living, heat-killed and UV-C stressed (oo)cysts were analyzed using these chemical probes. G. intestinalis in concentrated samples and stained with CFDA-SE were analysed by fluorescence imaging as well as by delayed fluorescence (DF) after UV-A and white-light excitation. The weak DF profiles were detected in photon-counting setups, in 8 series of tests for intact, for heat-killed and for UV-C-stressed samples are shown. Results show that fresh, i.e. living and viable (oo)cysts cannot be stained by the mentioned neither with CFDA-SE nor C12-resazurin and SYTOX Green dyes. Double-marked (oo)cysts are observed when C12-resazurin and SYTOX Green are applied to old cysts as well to dead ones. Aged samples show increasing number of stained organisms: 30-day-old with ~50% while samples older than 50 days with almost 100% marked. Intact samples present stronger fluorescence and DF than the stressed ones, with good replication after UV-A excitation. After excitation @365nm samples present DF better fitted by double exponential decay kinetics, with the decay constant k2 five times higher than the k1 constant. The procedure can be easily reproduced in 10 steps, taking around 1h of laboratorial work with purified samples
Doutorado
Agua e Solo
Doutor em Engenharia Agrícola
Liyanage, Lalith R. J. "Chlorine dioxide inactivation of Cryptosporidium parvum oocysts in water". Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk2/tape17/PQDD_0007/NQ29064.pdf.
Testo completoDas, Rony. "Cryptosporidium detection through antibody immobilization on a solid surface". Thesis, University of British Columbia, 2010. http://hdl.handle.net/2429/28932.
Testo completoHardy, Scott Andrew. "Effectiveness of static mixers for disinfection of cryptosporidium oocysts". Thesis, Georgia Institute of Technology, 1999. http://hdl.handle.net/1853/20925.
Testo completoPedraza-Diaz, Susana. "Molecular characterisation of cryptosporidium species involved in human infection". Thesis, Imperial College London, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.248120.
Testo completoMtambo, Mkumbukwa Madundo Angelo. "Cryptosporidium infection in cats : epidemioloigy and cross transmission studies". Thesis, University of Glasgow, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.384860.
Testo completoMarshall, James Spencer. "Cryptosporidium parvum : detection and distribution in two Yorkshire rivers". Thesis, University of Leeds, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.390895.
Testo completoMoriarty, Elaine Maria. "The detection and persistance of Cryptosporidium during beef processing". Thesis, University of Ulster, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.288825.
Testo completoMead, Jan Renee. "Cryptosporidium: Isolate variation and humoral responses to sporozoite antigens". Diss., The University of Arizona, 1988. http://hdl.handle.net/10150/184391.
Testo completo