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1

Cutarelli, Anna, Francesca Carella, Francesca De Falco, Bianca Cuccaro, Fabio Di Nocera, Donatella Nava, Gionata De Vico e Sante Roperto. "Detection and Quantification of Nocardia crassostreae, an Emerging Pathogen, in Mytilus galloprovincialis in the Mediterranean Sea Using Droplet Digital PCR". Pathogens 12, n. 8 (28 luglio 2023): 994. http://dx.doi.org/10.3390/pathogens12080994.

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Abstract (sommario):
Nocardia crassostreae is a novel pathogen responsible for infections in oysters (Crassostrea gigas) and mussels (Mytilus galloprovincialis). N. crassostreae is also responsible for nocardiosis both in immunocompetent and immunocompromised patients. We investigated N. crassostreae DNA in mussels grown in marine sites of the Mediterranean Sea in the Campania Region. We examined 185 mussel pooled samples by droplet digital PCR (ddPCR) and real-time quantitative PCR (qPCR), each pool composed of 10 mussels and 149 individual mussels. ddPCR detected N. crassostreae DNA in 48 mussel pooled samples and in 23 individual mussel samples. qPCR detected N. crassostreae DNA in six pooled samples and six individual mussel samples. The two molecular assays for the detection of N. crassostreae DNA showed significant differences both in the pooled and in individual samples. Our study demonstrated that ddPCR outperformed real-time qPCR for N. crassostreae DNA detection, thus confirming that ddPCR technology can identify the pathogens in many infectious diseases with high sensitivity and specificity. Furthermore, in individual mussels showing histological lesions due to N. crassostreae, the lowest copy number/microliter detected by ddPCR of this pathogen was 0.3, which suggests that this dose could be enough to cause infections of N. crassostreae in mussels.
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2

Elston, Ralph A. "Infectious diseases of the Pacific oyster, Crassostrea gigas". Annual Review of Fish Diseases 3 (gennaio 1993): 259–76. http://dx.doi.org/10.1016/0959-8030(93)90038-d.

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3

Schlenk, Daniel, e Donald R. Buhler. "Xenobiotic biotransformation in the pacific oyster (Crassostrea gigas)". Comparative Biochemistry and Physiology Part C: Comparative Pharmacology 94, n. 2 (gennaio 1989): 469–75. http://dx.doi.org/10.1016/0742-8413(89)90100-x.

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4

Lacoste, Arnaud, Shelagh K. Malham, Florence Gélébart, Anne Cueff e Serge A. Poulet. "Stress-induced immune changes in the oyster Crassostrea gigas". Developmental & Comparative Immunology 26, n. 1 (gennaio 2002): 1–9. http://dx.doi.org/10.1016/s0145-305x(01)00067-2.

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5

Sillanpää, J. Kirsikka, Henrik Sundh e Kristina S. Sundell. "Calcium transfer across the outer mantle epithelium in the Pacific oyster, Crassostrea gigas". Proceedings of the Royal Society B: Biological Sciences 285, n. 1891 (14 novembre 2018): 20181676. http://dx.doi.org/10.1098/rspb.2018.1676.

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Abstract (sommario):
Calcium transport is essential for bivalves to be able to build and maintain their shells. Ionized calcium (Ca 2+ ) is taken up from the environment and eventually transported through the outer mantle epithelium (OME) to the shell growth area. However, the mechanisms behind this process are poorly understood. The objective of the present study was to characterize the Ca 2+ transfer performed by the OME of the Pacific oyster, Crassostrea gigas, as well as to develop an Ussing chamber technique for the functional assessment of transport activities in epithelia of marine bivalves. Kinetic studies revealed that the Ca 2+ transfer across the OME consists of one saturable and one linear component, of which the saturable component fits best to Michaelis–Menten kinetics and is characterized by a K m of 6.2 mM and a V max of 3.3 nM min −1 . The transcellular transfer of Ca 2+ accounts for approximately 60% of the total Ca 2+ transfer across the OME of C. giga s at environmental Ca 2+ concentrations. The use of the pharmacological inhibitors: verapamil, ouabain and caloxin 1a1 revealed that voltage-gated Ca 2+ -channels, plasma-membrane Ca 2+ -ATPase and Na + /Ca 2+ -exchanger all participate in the transcellular Ca 2+ transfer across the OME and a model for this Ca 2+ transfer is presented and discussed.
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6

Potts, Robert W. A., Alejandro P. Gutierrez, Yennifer Cortés-Araya, Ross D. Houston e Tim P. Bean. "Developments in marine invertebrate primary culture reveal novel cell morphologies in the model bivalve Crassostrea gigas". PeerJ 8 (1 giugno 2020): e9180. http://dx.doi.org/10.7717/peerj.9180.

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Abstract (sommario):
Cell culture provides useful model systems used in a wide range of biological applications, but its utility in marine invertebrates is limited due to the lack of immortalised cell lines. Primary cell and tissue cultures are typically used but remain poorly characterised for oysters, which can cause issues with experimental consistency and reproducibility. Improvements to methods of repeatable isolation, culture, and characterisation of oyster cells and tissues are required to help address these issues. In the current study, systematic improvements have been developed to facilitate the culture of primary cells from adult Pacific oyster tissues and identify novel cell morphologies that have not been reported previously. Cultures analysed by light microscopy, qPCR, and live cell imaging demonstrated maintenance of live, metabolically active Pacific oyster cells for several weeks post-explant. Interestingly, whole hearts dissected from adult oysters were found to continue contracting rhythmically up to 8 weeks after being transferred to a tissue culture system. Mantle tissue explants were also actively moving in the culture system. These improvements in primary cell culture of bivalves may be beneficial for research in ecotoxicology, virology, immunology, and genetic resistance to disease.
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7

Bachère, E., D. Hervio, E. Mialhe e H. Grizel. "Evidence of neutralizing activity against T3 coliphage in oyster Crassostrea gigas hemolymph". Developmental & Comparative Immunology 14, n. 3 (giugno 1990): 261–68. http://dx.doi.org/10.1016/0145-305x(90)90017-9.

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8

Tirapé, Ana, Coralie Bacque, Raphaël Brizard, Franck Vandenbulcke e Viviane Boulo. "Expression of immune-related genes in the oyster Crassostrea gigas during ontogenesis". Developmental & Comparative Immunology 31, n. 9 (2007): 859–73. http://dx.doi.org/10.1016/j.dci.2007.01.005.

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9

Wang, Weilin, e Linsheng Song. "The diverse functions of DM9CPs in immune response of oyster Crassostrea gigas". Developmental & Comparative Immunology 148 (novembre 2023): 105006. http://dx.doi.org/10.1016/j.dci.2023.105006.

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10

Waechter, Magali, Frédérique Le Roux, Jean-Louis Nicolas, Éric Marissal e Franck Berthe. "Caractérisation de bactéries pathogènes de naissain d’huître creuse Crassostrea gigas". Comptes Rendus Biologies 325, n. 3 (marzo 2002): 231–38. http://dx.doi.org/10.1016/s1631-0691(02)01428-2.

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11

Song, Ying, Xiaorui Song, Dan Zhang, Ying Yang, Lingling Wang e Linsheng Song. "An HECT domain ubiquitin ligase CgWWP1 regulates granulocytes proliferation in oyster Crassostrea gigas". Developmental & Comparative Immunology 123 (ottobre 2021): 104148. http://dx.doi.org/10.1016/j.dci.2021.104148.

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12

Qiao, Xue, Yanan Zong, Zhaoqun Liu, Yuanmei Li, Jihan Wang, Lingling Wang e Linsheng Song. "A novel CgIFNLP receptor involved in regulating ISG expression in oyster Crassostrea gigas". Developmental & Comparative Immunology 124 (novembre 2021): 104206. http://dx.doi.org/10.1016/j.dci.2021.104206.

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13

Bachère, Evelyne, Dominique Chagot e Henri Grizel. "Separation of Crassostrea gigas hemocytes by density gradient centrifugation and counterflow centrifugal elutriation". Developmental & Comparative Immunology 12, n. 3 (giugno 1988): 549–59. http://dx.doi.org/10.1016/0145-305x(88)90071-7.

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14

Jia, Yunke, Bin Yang, Wenjing Dong, Zhaoqun Liu, Zhao Lv, Zhihao Jia, Limei Qiu, Lingling Wang e Linsheng Song. "A serotonin receptor (Cg5-HTR-1) mediating immune response in oyster Crassostrea gigas". Developmental & Comparative Immunology 82 (maggio 2018): 83–93. http://dx.doi.org/10.1016/j.dci.2017.12.029.

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15

Jin, Yuhao, Xue Qiao, Xiaojing Lv, Weilin Wang, Sicong Wang, Yuqian Gao, Lingling Wang e Linsheng Song. "A conserved RNAi molecule Ago2 involved in antiviral immunity of oyster Crassostrea gigas". Developmental & Comparative Immunology 142 (maggio 2023): 104668. http://dx.doi.org/10.1016/j.dci.2023.104668.

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16

Terahara, Kazutaka, Keisuke G. Takahashi e Katsuyoshi Mori. "Apoptosis by RGD-containing peptides observed in hemocytes of the Pacific oyster, Crassostrea gigas". Developmental & Comparative Immunology 27, n. 6-7 (giugno 2003): 521–28. http://dx.doi.org/10.1016/s0145-305x(02)00159-3.

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17

Barreau-Roumiguière, Catherine, Caroline Montagnani e Jean-Michel Escoubas. "Characterization of a Tal/SCL-like transcription factor in the pacific oyster Crassostrea gigas". Developmental & Comparative Immunology 27, n. 9 (ottobre 2003): 793–800. http://dx.doi.org/10.1016/s0145-305x(03)00075-2.

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18

Hervio, D., E. Bachere, E. Mialhe e H. Grizel. "10.3 Chemiluminescent responses of Ostrea edulis and Crassostrea gigas hemocytes to Bonamia ostreae (Ascetospora)". Developmental & Comparative Immunology 13, n. 4 (settembre 1989): 449. http://dx.doi.org/10.1016/0145-305x(89)90176-6.

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19

Olafsen, Jan A., Thelma C. Fletcher e Patrick T. Grant. "Agglutinin activity in pacific oyster (Crassostrea gigas) hemolymph following in vivo Vibrio anguillarum challenge". Developmental & Comparative Immunology 16, n. 2-3 (marzo 1992): 123–38. http://dx.doi.org/10.1016/0145-305x(92)90013-3.

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20

Thomas-Guyon, Hélène, Béatrice Gagnaire, Anne Bado-Nilles, Karine Bouilly, Sylvie Lapègue e Tristan Renault. "Detection of phenoloxidase activity in early stages of the Pacific oyster Crassostrea gigas (Thunberg)". Developmental & Comparative Immunology 33, n. 5 (maggio 2009): 653–59. http://dx.doi.org/10.1016/j.dci.2008.11.011.

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21

Sun, Ying, Zhi Zhou, Lingling Wang, Chuanyan Yang, Shuai Jianga e Linsheng Song. "The immunomodulation of a novel tumor necrosis factor (CgTNF-1) in oyster Crassostrea gigas". Developmental & Comparative Immunology 45, n. 2 (agosto 2014): 291–99. http://dx.doi.org/10.1016/j.dci.2014.03.007.

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22

Li, Yiqun, Shuai Jiang, Meijia Li, Lusheng Xin, Lingling Wang, Hao Wang, Limei Qiu e Linsheng Song. "A cytokine-like factor astakine accelerates the hemocyte production in Pacific oyster Crassostrea gigas". Developmental & Comparative Immunology 55 (febbraio 2016): 179–87. http://dx.doi.org/10.1016/j.dci.2015.10.025.

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23

Song, Kai, Yingxiang Li, Baoyu Huang, Li Li e Guofan Zhang. "Genetic and evolutionary patterns of innate immune genes in the Pacific oyster Crassostrea gigas". Developmental & Comparative Immunology 77 (dicembre 2017): 17–22. http://dx.doi.org/10.1016/j.dci.2017.07.012.

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24

Yang, Yuehong, Xue Qiao, Simiao Yu, Xinyu Zhao, Yuhao Jin, Rui Liu, Jie Li, Lingling Wang e Linsheng Song. "A trace amine associated receptor mediates antimicrobial immune response in the oyster Crassostrea gigas". Developmental & Comparative Immunology 156 (luglio 2024): 105171. http://dx.doi.org/10.1016/j.dci.2024.105171.

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25

Leung, P. S. C., e K. H. Chu. "cDNA cloning and molecular identification of the major oyster allergen from the Pacific oyster Crassostrea gigas". Clinical & Experimental Allergy 31, n. 8 (agosto 2001): 1287–94. http://dx.doi.org/10.1046/j.1365-2222.2001.01165.x.

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26

Seo, Jung-Kil, Min Jeong Lee, Hye-Jin Go, Gun Do Kim, Hyun Do Jeong, Bo-Hye Nam e Nam Gyu Park. "Purification and antimicrobial function of ubiquitin isolated from the gill of Pacific oyster, Crassostrea gigas". Molecular Immunology 53, n. 1-2 (gennaio 2013): 88–98. http://dx.doi.org/10.1016/j.molimm.2012.07.003.

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27

Bachere, E., D. Hervio, E. Mialhe e H. Grizel. "4.6 Evidence for a neutralizing activity against T3 coliphage in the Crassostrea gigas oyster hemolymph". Developmental & Comparative Immunology 13, n. 4 (settembre 1989): 384. http://dx.doi.org/10.1016/0145-305x(89)90096-7.

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28

Roberts, Steven, Yannick Gueguen, Julien de Lorgeril e Frederick Goetz. "Rapid accumulation of an interleukin 17 homolog transcript in Crassostrea gigas hemocytes following bacterial exposure". Developmental & Comparative Immunology 32, n. 9 (gennaio 2008): 1099–104. http://dx.doi.org/10.1016/j.dci.2008.02.006.

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29

Itoh, Naoki, Ryo Kamitaka, Keisuke G. Takahashi e Makoto Osada. "Identification and characterization of multiple β-glucan binding proteins in the Pacific oyster, Crassostrea gigas". Developmental & Comparative Immunology 34, n. 4 (aprile 2010): 445–54. http://dx.doi.org/10.1016/j.dci.2009.12.003.

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30

Liu, Zhaoqun, Zhi Zhou, Lingling Wang, Shuai Jiang, Weilin Wang, Ran Zhang e Linsheng Song. "The immunomodulation mediated by a delta-opioid receptor for [Met5]-enkephalin in oyster Crassostrea gigas". Developmental & Comparative Immunology 49, n. 2 (aprile 2015): 217–24. http://dx.doi.org/10.1016/j.dci.2014.11.017.

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31

Xu, Jiachao, Shuai Jiang, Yiqun Li, Meijia Li, Qi Cheng, Depeng Zhao, Bin Yang, Zhihao Jia, Lingling Wang e Linsheng Song. "Caspase-3 serves as an intracellular immune receptor specific for lipopolysaccharide in oyster Crassostrea gigas". Developmental & Comparative Immunology 61 (agosto 2016): 1–12. http://dx.doi.org/10.1016/j.dci.2016.03.015.

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32

Lu, Guangxia, Zichao Yu, Mengmeng Lu, Dongyang Liu, Feifei Wang, Yichen Wu, Yu Liu, Chao Liu, Lingling Wang e Linsheng Song. "The self-activation and LPS binding activity of executioner caspase-1 in oyster Crassostrea gigas". Developmental & Comparative Immunology 77 (dicembre 2017): 330–39. http://dx.doi.org/10.1016/j.dci.2017.09.002.

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33

Liu, Zhaoqun, Lingling Wang, Yunchen Yan, Yan Zheng, Wenjing Ge, Meijia Li, Weilin Wang, Xiaorui Song e Linsheng Song. "D1 dopamine receptor is involved in shell formation in larvae of Pacific oyster Crassostrea gigas". Developmental & Comparative Immunology 84 (luglio 2018): 337–42. http://dx.doi.org/10.1016/j.dci.2018.03.009.

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34

Lv, Zhao, Limei Qiu, Zhaoqun Liu, Weilin Wang, Hao Chen, Yunke Jia, Zhihao Jia, Shuai Jiang, Lingling Wang e Linsheng Song. "Molecular characterization of a cathepsin L1 highly expressed in phagocytes of pacific oyster Crassostrea gigas". Developmental & Comparative Immunology 89 (dicembre 2018): 152–62. http://dx.doi.org/10.1016/j.dci.2018.08.014.

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35

Wang, Weilin, Xiaojing Lv, Zhaoqun Liu, Xiaorui Song, Qilin Yi, Lingling Wang e Linsheng Song. "The sensing pattern and antitoxic response of Crassostrea gigas against extracellular products of Vibrio splendidus". Developmental & Comparative Immunology 102 (gennaio 2020): 103467. http://dx.doi.org/10.1016/j.dci.2019.103467.

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36

Li, Meijia, Miren Dong, Weilin Wang, Huan Li, Zhaoqun Liu, Lingling Wang, Kejian Wang e Linsheng Song. "A membrane-bound dopamine β-hydroxylase highly expressed in granulocyte of Pacific oyster Crassostrea gigas". Developmental & Comparative Immunology 104 (marzo 2020): 103563. http://dx.doi.org/10.1016/j.dci.2019.103563.

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37

Lv, Xiaojing, Weilin Wang, Qi Zhao, Xue Qiao, Liyan Wang, Yunchen Yan, Shuo Han, Zhaoqun Liu, Lingling Wang e Linsheng Song. "A truncated intracellular Dicer-like molecule involves in antiviral immune recognition of oyster Crassostrea gigas". Developmental & Comparative Immunology 116 (marzo 2021): 103931. http://dx.doi.org/10.1016/j.dci.2020.103931.

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38

Liu, Yu, Weilin Wang, Qi Zhao, Pei Yuan, Jiaxin Li, Xiaorui Song, Zhaoqun Liu, Dewen Ding, Lingling Wang e Linsheng Song. "A DM9-containing protein from oyster Crassostrea gigas (CgDM9CP-3) mediating immune recognition and encapsulation". Developmental & Comparative Immunology 116 (marzo 2021): 103937. http://dx.doi.org/10.1016/j.dci.2020.103937.

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39

Terahara, Kazutaka, Keisuke G. Takahashi, Akifumi Nakamura, Makoto Osada, Masaki Yoda, Takachika Hiroi, Masatomo Hirasawa e Katsuyoshi Mori. "Differences in integrin-dependent phagocytosis among three hemocyte subpopulations of the Pacific oyster “Crassostrea gigas”". Developmental & Comparative Immunology 30, n. 8 (gennaio 2006): 667–83. http://dx.doi.org/10.1016/j.dci.2005.09.009.

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40

Wang, Huihui, Hong Yu, Qi Li e Shikai Liu. "Transcription Analysis for Core Networks of lncRNAs–mRNAs: Implication for Potential Role in Sterility of Crassostrea gigas". Biology 11, n. 3 (27 febbraio 2022): 378. http://dx.doi.org/10.3390/biology11030378.

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Abstract (sommario):
Long noncoding RNA (lncRNA), a type of non-protein-coding transcript, is emerging as a crucial regulator of gene expression. However, few roles of lncRNA in the reproductive process of the Pacific oyster (Crassostrea gigas) have been defined, especially in the regulatory mechanism of sterile triploids gametogenesis. To uncover the potential role of lncRNA, the gonads of diploids, sterile triploids, and partially sterile triploids underwent RNA sequencing. A total of 9618 reliable lncRNAs were identified. The target relationship between lncRNA and mRNA was predicted based on cis, trans, and antisense regulation with bioinformatic software. We chose differentially expressed lncRNAs and mRNAs when sterile triploids were compared to partially sterile triploids and diploids for subsequent functional enrichment analysis. Findings revealed that trans-regulatory lncRNAs might play a significant role in the gametogenesis of C. gigas. Combining pathway results, we constructed core networks of lncRNAs–mRNAs for triploid sterile females and hermaphrodites. Fifty-four genes related to cell division, germline-cell maintenance, and glycogen metabolism were found to be associated with sterility. A total of 28 candidate lncRNAs were predicted to trans-regulate these genes. We speculated that MSTRG.79882.3 and MSTRG.79882.4 for triploid sterile females and MSTRG.33704.1, MSTRG.63844.1, and MSTRG.5675.1 for hermaphrodites were highly important as they were predicted to regulate more sterility-specific genes than others. Our work collectively identified sterility-related lncRNAs and implicated the potential mechanism of lncRNA-mediated regulation in the gametogenesis of sterile triploid oysters.
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Bachere, Evelyne, Yannick Gueguen, Marcelo Gonzalez, Julien de Lorgeril, Julien Garnier e Bernard Romestand. "Insights into the anti-microbial defense of marine invertebrates: the penaeid shrimps and the oyster Crassostrea gigas". Immunological Reviews 198, n. 1 (aprile 2004): 149–68. http://dx.doi.org/10.1111/j.0105-2896.2004.00115.x.

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42

Li, Jiaxin, Weilin Wang, Qi Zhao, Siqi Fan, Yan Li, Pei Yuan, Lingling Wang e Linsheng Song. "A haemocyte-expressed Methyltransf_FA domain containing protein (MFCP) exhibiting microbe binding activity in oyster Crassostrea gigas". Developmental & Comparative Immunology 122 (settembre 2021): 104137. http://dx.doi.org/10.1016/j.dci.2021.104137.

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43

Zhang, Dan, Miren Dong, Xiaorui Song, Xue Qiao, Ying Yang, Simiao Yu, Wending Sun, Lingling Wang e Linsheng Song. "ROS function as an inducer of autophagy to promote granulocyte proliferation in Pacific oyster Crassostrea gigas". Developmental & Comparative Immunology 135 (ottobre 2022): 104479. http://dx.doi.org/10.1016/j.dci.2022.104479.

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44

Sun, Wending, Xiaorui Song, Miren Dong, Zhuyun Liu, Ying Song, Lingling Wang e Linsheng Song. "DNA binding protein CgIkaros-like regulates the proliferation of agranulocytes and granulocytes in oyster (Crassostrea gigas)". Developmental & Comparative Immunology 124 (novembre 2021): 104201. http://dx.doi.org/10.1016/j.dci.2021.104201.

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Lacoste, A., S. K. Malham, A. Cueff e S. A. Poulet. "Noradrenaline modulates hemocyte reactive oxygen species production via β-adrenergic receptors in the oyster Crassostrea gigas". Developmental & Comparative Immunology 25, n. 4 (maggio 2001): 285–89. http://dx.doi.org/10.1016/s0145-305x(00)00067-7.

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46

Liu, Dongyang, Qilin Yi, Yichen Wu, Guangxia Lu, Changhao Gong, Xiaorui Song, Jiejie Sun et al. "A hypervariable immunoglobulin superfamily member from Crassostrea gigas functions as pattern recognition receptor with opsonic activity". Developmental & Comparative Immunology 86 (settembre 2018): 96–108. http://dx.doi.org/10.1016/j.dci.2018.05.007.

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Chen, Honglin, Xiaohui Cai, Jing Fang, Ruixue Li, Caifeng Zhang, Hangna Qiu e Xinzhong Wu. "TLR mediated innate immunity was essential in defense against Vibrio alginolyticus for Crassostrea gigas digestive gland". Developmental & Comparative Immunology 148 (novembre 2023): 105027. http://dx.doi.org/10.1016/j.dci.2023.105027.

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48

Durland, Evan, Pierre De Wit e Chris Langdon. "Temporally balanced selection during development of larval Pacific oysters ( Crassostrea gigas ) inherently preserves genetic diversity within offspring". Proceedings of the Royal Society B: Biological Sciences 288, n. 1958 (settembre 2021): 20203223. http://dx.doi.org/10.1098/rspb.2020.3223.

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Abstract (sommario):
Balancing selection is one of the mechanisms which has been proposed to explain the maintenance of genetic diversity in species across generations. For species with large populations and complex life histories, however, heterogeneous selection pressures may create a scenario in which the net effects of selection are balanced across developmental stages. With replicated cultures and a pooled sequencing approach, we show that genotype-dependent mortality in larvae of the Pacific oyster ( Crassostrea gigas ) is largely temporally dynamic and inconsistently in favour of a single genotype or allelic variant at each locus. Overall, the patterns of genetic change we observe to be taking place are more complex than what would be expected under classical examples of additive or dominant genetic interactions. They are also not easily explained by our current understanding of the effects of genetic load. Collectively, temporally heterogeneous selection pressures across different larval developmental stages may act to maintain genetic diversity, while also inherently sheltering genetic load within oyster populations.
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49

Tran, Damien, Mickael Perrigault, Pierre Ciret e Laura Payton. "Bivalve mollusc circadian clock genes can run at tidal frequency". Proceedings of the Royal Society B: Biological Sciences 287, n. 1918 (8 gennaio 2020): 20192440. http://dx.doi.org/10.1098/rspb.2019.2440.

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Abstract (sommario):
Marine coastal habitats are complex cyclic environments as a result of sun and moon interactions. In contrast with the well-known circadian orchestration of the terrestrial animal rhythmicity (approx. 24 h), the mechanism responsible for the circatidal rhythm (approx. 12.4 h) remains largely elusive in marine organisms. We revealed in subtidal field conditions that the oyster Crassostrea gigas exhibits tidal rhythmicity of circadian clock genes and clock-associated genes. A free-running (FR) experiment showed an endogenous circatidal rhythm. In parallel, we showed in the field that oysters' valve behaviour exhibited a strong tidal rhythm combined with a daily rhythm. In the FR experiment, all behavioural rhythms were circatidal, and half of them were also circadian. Our results fuel the debate on endogenous circatidal mechanisms. In contrast with the current hypothesis on the existence of an independent tidal clock, we suggest that a single ‘circadian/circatidal’ clock in bivalves is sufficient to entrain behavioural patterns at tidal and daily frequencies.
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Xin, Lusheng, Chang Liu, Huan Zhang, Limei Qiu, Lingling Wang e Linsheng Song. "The characterization of an interleukin-12 p35 homolog involved in the immune modulation of oyster Crassostrea gigas". Developmental & Comparative Immunology 123 (ottobre 2021): 104145. http://dx.doi.org/10.1016/j.dci.2021.104145.

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