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1
Perrin, Adrien. "Biocicatrisation des structures en béton par injection et aspersion de milieux rhéofluidifiants inoculés". Electronic Thesis or Diss., Ecole nationale supérieure Mines-Télécom Lille Douai, 2022. http://www.theses.fr/2022MTLD0003.
Abstract (sommario):
Le béton, le matériau de construction le plus utilisé au monde, peut s’altérer au contact de son environnement, conduisant à générer des fissures et à réduire la durabilité des ouvrages. La méthode de réparation des fissures fines la plus utilisée actuellement est l’injection de résines (époxy, polyuréthanes, etc.). Cependant, il s’agit d’une technique polluante, de courte durée et parfois peu compatible avec le béton. L’objectif de cette thèse est de développer une méthode de réparation biologique applicable à des ouvrages réels en injectant une formulation inoculée pour colmater une fissure avec un composite formé de carbonate de calcium biosourcé et de biofilm. Deux éléments ont été développés : un inoculum microbien et un outil prédictif permettant la productionde formulations épaissies rhéofluidifiantes adaptées à la taille de la fissure à traiter.L’inoculum microbien est un consortium de micro-organismes indigènes adaptés aux matériaux cimentaires. Il a une croissance rapide et permet la production significative de calcite et de vatérite à partir de lactate de calcium dans les conditions environnementales d’un ouvrage en béton (20°C et pH de surface alcalin). L’outil prédictif a permis la production de formulations injectables et biocompatibles dans des fissures allant de 40 à 1000 µm. Une formulation sélectionnée, en association avec le consortium microbien, a été injectée dans des mortiers fissurés en conditions de laboratoire (20°C, 100% d’humidité relative). Une injection de la formulation épaissie inoculée a permis de réduire l’ouverture apparente de fissures fines (de l’ordre de 100 µm) de 84-90% et de fissures moyennes (250 et 450 µm) de 77-78%. Le système de biocicatrisation a été utilisé pour bioréparer deux fissures de l’ordre de 400 µm dans une dalle en béton fissurée (1 x 2 x 0,15 m) en conditions de laboratoire (20°C). Les résultats obtenus ont montré une réduction de la perméabilité de l’eau à 99% pour les deux fissures avec une confirmation que le matériau de colmatage est bien de nature biologique. Deux méthodes d’injection adaptées à des fissures sèches et humides ont été développées. Elles ont permis de tester le système de biocicatrisation sur des fissures d’ouvrages réels : la maquette VeRCoRs d’EDF (Electricité De France) ) Moret-Loing-et Orvane, France (fissures sèches), qui reproduit à l’échelle 1/3 une enceinte de réacteur nucléaire et un barrage de la centrale hydroélectrique d’Hydro-Sherbrooke à Weedon, CANADA (fissures humides). Ces deux essais ont montré des résultats prometteurs et constituent une preuve de concept de cette technique de biocicatrisation par injectionConcrete, the most widely used construction material, can deteriorate on contact with its environment, leading to cracks which may negatively impact its durability. The most commonly used method for repairing fine cracks is the injection of resins (epoxy, polyurethane, etc.). However, these potentially polluting materials can have a limited lifespan and are sometimes incompatible with concrete. The objective of this thesis is to develop a biological crack repair method for concrete structures. Crack sealing is achieved by injecting an inoculated thickened liquid, leading to the production of bio-based calcium carbonate and biofilm into the crack. The development has two main innovations : a specific microbial inoculum and a predictive model for the design and the production of shear thinning injection liquid specifically optimized for the opening of the crack to be repair.The microbial inoculum is a consortium of native microorganisms adapted to cementitious materials. The consortium grows rapidly and produces significant amount of calcite and vaterite from the metabolization of calcium lactate under typical environmental conditions found in a crack (humidity, 20°C and alkaline pH). The predictive model has demonstred its effectiveness in designing injectable and biocompatible formulations in cracks with opening ranging from 40 to 1000 µm. A selected formulation inoculated with the microbial consortium was injected into mortar samples with a range of cracks opening, and then stored under laboratory conditions (20°C, 100% relative humidity). For fine cracks (approximately 100 µn) injection of the inoculated thickened formulation reduced the apparent crack opening by 84-90%. For larger cracks (250 and 450 µm), the apparent crack opening was reduced by 77%. The same biocicatrisation system was used to bio-repair two 400 µm cracks in a concret slab (1 x 2 x 0,5) stored in laboratory conditions (20°C). Biotreatment of the cracks resulted in a 99% reduction in water permeability for both cracks, with the confirmation that some scaling materials formed in the cracks are the result of a biological process. Two injection methods suitable for dry and wet cracks have been developed for bio-treatement tests performed on larger scale concrete structures : for dry cracks, the EDF VeRCoRs mock-up, which is 1/3 scale nuclear reactor enclosure ; anf for wet cracks, a dam in the hydroelectric power plant of Weedon, Canada. These two on-site trials have produced promising results and are a significant contribution to the proof of concept of this new approach for biotreament of cracks
2
Maretto, Andrea <1994>. "Fiscalità dei consorzi tra imprese e delle società consortili". Master's Degree Thesis, Università Ca' Foscari Venezia, 2019. http://hdl.handle.net/10579/14323.
Abstract (sommario):
L’elaborato tratterà in primo luogo degli aspetti civilistici del contratto di consorzio e delle società consortili. L’attenzione si sposterà in seguito sul loro trattamento fiscale, in particolare verranno esaminati i seguenti temi: IVA, imposte sul reddito, agevolazioni fiscali.
3
Taole, Nthabiseng. "Evaluation of the Innopac Library system in selected consortia and libraries in the southern African region : implications for the Lesotho Library consortium". Thesis, University of Pretoria, 2008. http://hdl.handle.net/2263/23861.
Abstract (sommario):
Resource sharing is considered to be one of the most important pillars of library service, because no single library can meet all the needs of its users. Libraries have always cooperated to meet the increasing demands of users by sharing their resources. In the past few decades, the need to establish library consortia emerged more strongly as libraries began to take advantage of technology to improve access to information and service delivery. There has been a notable increase in the formation of library consortia on the African continent. South Africa has taken the lead both in the amount of established consortia and the number of member libraries within them. This development accompanied the implementation of common library systems in consortia, where a single system is adopted by all member libraries. In the Southern African region, the library system called INNOPAC/Millennium Pac has already been adopted by consortia and libraries in Botswana, Mozambique, Namibia, South Africa, and Zimbabwe. The recently-established Lesotho Library Consortium (LELICO) also recognized the need for a comprehensive investigation to identify a common system that will effectively meet the needs of its member libraries. The purpose of this study was to analyze the successes and limitations of the INNOPAC library system operating in consortia and libraries in the Southern African region, in order to assess its suitability for LELICO. The study focused on two South African consortia (The Gauteng and Environs Library Consortium – GAELIC, and The Free State Library and Information Consortium - FRELICO), two university libraries (Namibia and Zimbabwe) and one agricultural college library (Botswana) in the Southern African region that use the system. A special emphasis was the criteria of assessment that would apply to a small, multi-type consortium in a developing country like Lesotho. Data was collected through a literature search, questionnaires, interviews, site visits, and analysis of policy and institutional documents. The target groups of the study were the library managers, system managers, and library professionals of selected GAELIC and FRELICO libraries, and the system managers of the three selected libraries in the region. The study found that the INNOPAC library system is performing satisfactorily in the chosen consortia and libraries, and that it has a positive impact on them. It performed to a high standard in all the key areas, and this may be attributed to keeping abreast of the latest developments in the library world, and offering a range of services that meet the needs of libraries. The study found further that the INNOPAC library system contributed towards increased productivity, improved customer services, and better decision making in the two consortia. However, direct access to members’ holdings was restricted by a decentralized server model adopted by these consortia. This and other lessons shaped a proposal for the implementation and management of the INNOPAC library system in LELICO. A proposed model recommends a central server as a more cost-effective management solution. The model also explains the mode of operation by member libraries and the coordinated structures that would implement and manage the INNOPAC library system, adapted to the specific requirements of a small, multi-type consortium in a developing country like Lesotho. Given its successful performance in consortia and libraries across Southern African countries, the study recommends further research into the advantages and challenges of INNOPAC for wider regional library cooperation.Thesis (DPhil)--University of Pretoria, 2009.
Information Science
unrestricted
4
Taole, Nthabiseng. "Evaluation of the INNOPAC Library System in selected consortia and libraries in the Southern African region implications for the Lesotho Library Consortium /". Pretoria : [s.n.], 2009. http://upetd.up.ac.za/thesis/available/etd-04082009-204135/.
5
Arora, Jagdish, e Pawan Agrawal. "Indian Digital Library in Engineering Science and Technology (INDEST) Consortium: Consortia-Based Subscription to Electronic Resources for Technical Education System in India: A Government of India Initiative". Information and Library Network Centre, 2003. http://hdl.handle.net/10150/105608.
Abstract (sommario):
The Ministry of Human Resource Development (MHRD) has set-up a â Consortia-based Subscription to Electronic Resources for Technical Education System in Indiaâ on the recommendations made by the Expert Group appointed by the ministry. The consortium is named as the Indian National Digital Library in Science and Technology (INDEST) Consortium. The INDEST Consortium has commenced its operation since Dec., 2002 through its headquarters at the IIT Delhi. The Consortium subscribes to full-text electronic resources and bibliographic databases for 38 leading engineering and technological institutions in India including IITs (7), IISc (1), NITs / RECs (17), IIMs (6) and a few other institutions directly funded by the Ministry of Human Resource Development (MHRD). While the expenditure on electronic resources proposed for subscription under the consortium for these 38 institutions are being met from the funds made available by the MHRD, the consortium being an open-ended proposition, welcomes all other institutions to join it on their own for sharing benefits it offers in terms of highly discounted subscription rates and better terms of agreement with the publishers. Moreover, beneficiary institutions may also subscribe to additional electronic resources through the consortium that are not being funded by the MHRD. This article introduces the INDEST Consortium, its activities and services.
6
Chenut, Charles-Henry. "Le contrat de consortium". Paris 10, 2001. http://www.theses.fr/2001PA100110.
Abstract (sommario):
Le contrat de consortium est un accord de coopération, plus couramment dénommé, groupement momentané d'entreprises, conclu entre deux ou plusieurs personnes physiques et/ou morales qui s'engagent à exécuter, chacune en ce qui concerne, des prestations distinctes en vue d'une opération commune déterminée. Cette convention ne donne pas naissance à une société et ne confère pas au groupement qu'il crée la personnalité morale. D'un point de vue pratique, le consortium est largement, et depuis longtemps, usité. Or, sous l'angle strictement du droit, le consortium ne répond en France à la grande différence de nombreux pays étrangers- à aucun statut précis. D'aucuns considèrent alors qu'il s'agit d'un contrat innommé. Il ne convient pas pour autant d'abandonner le consortium au triste sort de droit commun des obligations, mais davantage de revendiquer son originalité conceptuelle et son unicité juridique. Nous pensons, en effet, que ce contrat a acquis, après une enfance tumultueuse, une maturité telle qu'il peut être élevé au rang des contrats spéciaux. Afin de poursuivre cet objectif, une approche analytique du consortium est proposée dans un premier temps. Celle-ci envisage, d'une part, de rechercher la nature de l'accord. Sa présentation-son appréhension dans l'univers juridique-précède sa qualification, étape liminaire mais fondamentale de la reconnaissance de la convention et de sa consécration. D'autre part, le consortium offre un régime de droit particulier. Ce contrat connaît en effet, de sa phase de conclusion jusqu'à celle de son extinction, un cortège de règles qui lui sont propres. Dans un second temps, une comparaison de l'accord de consortium avec d'autres formes de coopération met un terme à tout risque de confusion entre ces multiples contrats unissant divers partenaires, qu'il s'agisse de ceux, tout d'abord, qui réalisent directement le marché-tels les contrats de sous-traitance, de société en participation et de société créée de fait -ou de ceux, ensuite, qui sont en marge de ce dernier-comme l'association de la loi 1901 ou le groupement d'intérêt économique. L'approche comparative de cette convention est une étape utile à sa découverte et contribue in fine à sa connaissance parfaite. L'analyse de l'accord se compose autant de la maîtrise de sa notion que de sa compositionThe consortium contract is a particular partnership. Although there is no legal definition in French law, a consortium - which is a kind of contractual joint-venture - may be described as an agreement of cooperation between independent parties (generally of similar economic weight) who venture into a common objective and who negociate as equals. Contractual joint ventures generally do not include the main activities of the members of the consortium but are used either for specific operations or for ancillary activities where the joint venturers need assistance. Consortium contracts should be selected when a common entity structure is not justified because the common activities are to be carried out on a short term or experimental basis, do not require significant financing or for confidentiality reasons. A consortium is created by a direct contractual relationship between joint venturers through one or several contrats without formation of a entity such as a partnership or corporation. It is in fact a simple contrat of cooperation between two or several partners pursuing the same objectives. However, in addition to a statement on their objectives and the mean to be taken to achieve them, the parties must be define 'who does what' in a very precise manner as by definition there is no common entity through which the parties' joint endeavours will be channelled. This consitutes a fundamental différence with the other types of joint ventures which give rise to legal entities having a independent legal statuts. In spite of the absence of a separate legal entity, the members of the consortium have certain obligations, which, if not complied with pursuant of the joint venture contrats, will entail general penalties under the applicable contractual law and possibly specific penalties which may be stipulated in such contrats, enforceable through court proceedings or, more often, arbitration proceedings. Under french law, consortium contract is known as undesignated contrat as it is not specifically categorised in French civil code. Contractual joint ventures are diverse and numerous. However, an analysis of joint venture contracts reveals that they have common points, whaterver the object of the consortium may be. Then, a specific status of this particular cooperation appears. It is the aim of our work to demonstrate its existence
7
Tandon, Aditya. "Transferring technology from R&D consortia to participating shareholders, a study of Canadian consortia". Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp01/MQ32421.pdf.
8
Roper, Angela Jane. "Hotel consortia : strategies and structure : an analysis of the emergence of hotel consortia as transorganisational forms". Thesis, University of Huddersfield, 1992. http://eprints.hud.ac.uk/id/eprint/4847/.
9
Gaulin, Jean-Philippe. "Selective caffeine removal by microbial consortia". Thesis, McGill University, 2003. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=80272.
Abstract (sommario):
Coffee processing presents a considerable waste disposal problem, mainly because of the large volumes generated and the chemical composition of the by-products, particularly the caffeine levels. The use of Pseudomonas putida IF-3, a caffeine-degrading microorganism, in a microbial consortium for bioremediation of caffeine found in coffee wastes was investigated. Caffeine degradation was observed in fed-batch reactor experiments with caffeine as sole source of carbon and nitrogen. Metabolic regulation and caffeine removal potential by Pseudomonas putida IF-3 were investigated by supplementing with other nutrient sources. Diauxic growth was not observed. Nitrogen release from caffeine breakdown was found to be rate-limiting.Effects of caffeine on microbial consortia were studied using denaturing gradient gel electrophoresis (DGGE), providing a community-scale view of changes in microbial consortia upon caffeine addition. Surprisingly, caffeine removal was achieved indigenously by the microbial consortium. Principal component analysis was used to analyze differences in DGGE banding patterns between control and caffeine-exposed mixed cultures.
10
Poland, Mark W. "Factors associated with statutory consortium effectiveness : a case study of one Virginia consortium for continuing higher education". W&M ScholarWorks, 1986. https://scholarworks.wm.edu/etd/1539618555.
Abstract (sommario):
The purpose of this study was to determine if a statutory higher education consortium possesses the attributes generally associated with effective voluntary higher education consortia. Also, the research attempted to discover if there are other attributes which would contribute to the effectiveness of statutory higher education consortia.;A review of the literature on voluntary consortia revealed that voluntary higher education consortia regarded as effective generally (1) have clear, concise goals; (2) have an open, two-way communication system; (3) are supported by the presidents of the member institutions; (4) engage in incremental planning; (5) have an effective administrator/director; and (6) are perceived as useful by the members.;Using case study methodology, one Virginia Consortium for Continuing Higher Education was examined to determine if the effectiveness attributes of voluntary consortia were present in this statutory higher education consortium and to determine if other attributes might also be essential for statutory consortium effectiveness. Interviews were held with the key people associated with the consortium under study. Consortium documents located at each interview site were examined. The data were evaluated through triangulation techniques.;This statutory consortium did have a clear, concise mission and did have an open, two-way communication system. Presidential support was found to be limited and the consortium's usefulness to its members are restricted to secondary factors. The consortium leadership was viewed as effective although within a more narrow conception of leadership than that generally found in an effective voluntary consortium. Finally, evidence indicated that the consortium had no incremental planning process.;This research suggests that, to encourage the effectiveness of a statutory higher education consortium, the establishing agency should insure that several criteria are satisfied: (1) institutions must want to cooperate, (2) the reasons for cooperation must be clear, (3) incentives for cooperation must be provided, (4) all members must share equitably in the cooperative endeavor, (5) communication must be open and two-way, (6) the member institution presidents must support the consortium, (7) a planning process must be put in place, (8) the consortium administrator must be an effective leader, and (9) the external population the consortium plans to serve must be encouraged to use the consortium's services.
11
Amouric, Agnès. "Biodiversité d'un consortium microbien et études génétiques de SP2B, un actinomycète isolé du consortium, capable de dégrader l'hexane". Aix-Marseille 1, 2006. http://www.theses.fr/2006AIX11007.
Abstract (sommario):
Un consortium microbien capable de dégrader l'essence a été réadapté en culture liquide sur essence sans plomb/ méthyl tert-butyl éther. Les cinétiques de dégradation de l'hexane par ce consortium ont été étudiées en microcosme liquide et dans un biofiltre de paillasse. Sur les 28 derniers jours en biofiltre, le taux de production de CO2 et la capacité d'élimination du consortium se sont stabilisés. Un échantillon a été prélevé pour caractériser la biodiversité, et la comparer à celles du consortium initial réadapté sur essence, et de ce même consortium adapté sur l'hexane en microcosme liquide. Des différences significatives entre les populations ont été observées, indiquant une adaptation probable en fonction des conditions de cultures. Trois souches bactériennes dont deux Actinomycètes ont été isolées à partir du consortium "hexane liquide". L'une d'entre elle, SP2B, dégrade l'hexane et d'autres alcanes à courte chaîne contrairement à la souche type phylogénétiquement la plus proche (Rhodococcus ruber DSM 43338T). Le profil d'acides gras cellulaires sur différents alcanes et la croissance sur les intermédiaires du métabolisme de l'hexane similaires pour les deux souches n'ont pas permis d'expliquer la différence de phénotype. De plus, un gène identique alkB a été identifié dans les deux souches. Il code pour une alcane monooxygénase qui pourrait être impliquée dans la dégradation des alcanes à longues chaînes. La spécificité de dégradation de SP2B pourrait être liée à la présence d'une autre copie de alkB identifiée. Des études pour caractériser ce nouveau gène ainsi que la régulation de tous ceux impliqués dans la dégradation de l'hexane, sont en coursA gasoline-degrading consortium was readapted in liquid using gasoline/methyl tert-butyl ether and was tested for its hexane-degradation kinetics in liquid microcosm and in a bench-scale biofilter. On the 28 last days, Elimination Capacity and CO2 production rate in the biofilter remained constant. A sample was collected to characterize the biodiversity and compare it with that of the initial gasoline consortium and the same consortium adapted on hexane in liquid microcosm. Significant differences between the populations were observed, indicating a probable adaptation to the culture conditions. Three strains including two Actinomycetes were isolated from the consortium. One of them, SP2B, degrade hexane and other short-alkane compared with the type strain phylogenetically related (Rhodococcus ruber DSM 43338T). Cellular fatty-acid profiles on various alkanes, as well as the growth in the presence of hexane-metabolism intermediaries identical for both strains didn't allow to understand the phenotypic differences. Moreover, an identical gene, alkB, was found in both strains. It codes for an alkane monooxygenase, which product is probably involved in long-chain-alkane degradation. Another alkB gene, identified in SP2B, could therefore be involved in the degradation specificity of the strain. Studies are in progress in order to precisely characterize this new gene and to understand the regulations on the expression of all the elements involved in hexane degradation
12
TAKABE, Tetsuko. "Biotechnology of Crop Production and International Consortium". 名古屋大学農学国際教育協力研究センター, 2004. http://hdl.handle.net/2237/8933.
13
Ivorra, i. Castellà Núria. "Metal induced succession in benthic diatom consortia". [S.l. : Amsterdam : s.n.] ; Universiteit van Amsterdam [Host], 2000. http://dare.uva.nl/document/56237.
14
Vogt, Oliver. "Collaborative electronic purchasing within an SME consortium". Thesis, Durham University, 2008. http://etheses.dur.ac.uk/1328/.
Abstract (sommario):
The main function of purchasing is to assure the supply with required goods and services. Large organisations have both finances and knowledge to implement optimised purchasing resources, typically using information and communications technology (ICT) to improve efficiency. On the contrary, within individual small and medium sized enterprises electronic purchasing is conducted predominately through supplier's sales web sites.
15
Bidgood, Lee. "Grounds and Consorts". Digital Commons @ East Tennessee State University, 2014. https://dc.etsu.edu/etsu-works/1051.
Abstract (sommario):
The concert features an eclectic variety of blues, rags, fiddle tunes and consort music arranged for both historic and modern instruments. The program includes works by numerous composers, from Johann Groh and Thomas Lupo, who were born in the late 16th century, to George Linus Cobb and Doc Roberts of the early 20th century.
16
Glaeser, Jens. "In situ Metabolism and Biogeography of Phototrophic Consortia". Diss., lmu, 2004. http://nbn-resolving.de/urn:nbn:de:bvb:19-19707.
17
Mashaphu, Nthabiseng. "The microbial composition of a natural methanogenic consortium". Thesis, University of the Western Cape, 2005. http://etd.uwc.ac.za/index.php?module=etd&.
Abstract (sommario):
Wetlands account for approximately 20% of annual global methane emissions. Many wetlands receive inputs of organic matter, nutrients, metals and various toxic compounds from adjacent agricultural and industrial areas. The present study aimed to investigate the microbial composition of a natural methanogenic consortium. A consortium-based molecular approach to study diversity of methanogenic microbial communities in a natural wetland at the primary inflow was used. Key microorganisms of a nethane producing consortium were identified. Extracted high molecular mss DNA ws analysed by PCR combined with denaturing gradient gel electrophoresis and subsequent sequencing of 16S rDNA. This study was also aimed to identify syntrophic microorganisms in the wetland system. The data obtained suggest a well established syntrophic relationship within the wetland.
18
Merkx, Joost, e Philipp Gresse. "Purchasing Consortia of Transportation Services in Humanitarian Logistics". Thesis, Internationella Handelshögskolan, Högskolan i Jönköping, IHH, Företagsekonomi, 2012. http://urn.kb.se/resolve?urn=urn:nbn:se:hj:diva-18617.
Abstract (sommario):
Purpose - The thesis aims to provide a framework of measuring the performance of pur-chasing consortia humanitarian sector. The performance measurement seeks to identify the competitive advantages of being a member of purchasing consortia during purchasing activities of transportation ervices. Design/methodology/approach – The research is based on a qualitative study with de-ductive and explanatory approach. Semi-structured interviews with experts within the humanitarian sector have been conducted to gain necessary and credible data. Findings – It has been identified that purchasing consortia do not mutually operate physical purchasing activities. However, the consortia performance measurement framework, derived from the resource-based view, illustrates that being a member gen-erates the capabilities of decreased purchasing complexity, learning capabilities and ca-pacity sharing. These capabilities have a significant impact on the purchasing process for transportation services and lead to the competitive advantages of (1) having access to extensive knowledge, (2) reducing operational efforts, (3) reducing lead-time and (4) improving learning procedures. Research limitations/implications – As the literature on humanitarian logistics is still in its infancy, further applications from the commercial sector were considered to strengthen the results of the framework. Practical implications - The extent of collaboration of purchasing consortia indicates that managers of HOs are able to gain significant expertise and offers advantageous opportunities in handling purchasing activities in humanitarian logistics. The implications cover strategic as well as operational issues. Originality/value – The thesis gives an insight about practical purchasing operations of existing consortia and detects previously unknown aspects within the literature of humanitarian logistics.
19
Bensaid, Anouar. "Comportement social d'un consortium bactérien dans un chémostat". Doctoral thesis, Universite Libre de Bruxelles, 2000. http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/211762.
20
Bradbury, Seth R. "Bacterial and Fungal Consortia in Early Childhood Caries". The Ohio State University, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=osu1593627801051632.
21
Jiranuntipon, Suhuttaya. "Décoloration d’effluents de distillerie par un consortium microbien". Thesis, Toulouse, INPT, 2009. http://www.theses.fr/2009INPT009G/document.
Abstract (sommario):
Les effluents de distillerie de mélasse de canne à sucre génèrent une pollution environnementale due à, d’une part de grands volumes et d’autres part à la présence de composés de couleur brune foncée, connus sous le nom de mélanoïdines. Dans cette étude, un consortium bactérien CONS8 isolé dans des sédiments de chute d'eau a été choisi comme consortium apte à la décoloration de la mélasse. On a montré que le consortium CONS8 pouvait décolorer, trois eaux usées synthétiques différentes, élaborées respectivement à base de Viandox (13,48% v/v), d’eau usée de mélasse de betterave (41,5% v/v) ou d’eau usée de mélasse de canne à sucre (20% v/v). Les décolorations obtenues en 2 jours seulement, en fioles d’Erlenmeyer sont respectivement de 9,5, à 8,02 et à 17,5%. Quatre bactéries prédominantes ont été identifiées dans le consortium CONS8 par l'analyse de l'rADN 16S. Sur la base de cette identification, et afin de réaliser la décoloration la plus élevée, un consortium bactérien artificiel MMP1 a été reconstruit avec Klebsiella oxytoca, Serratia mercescens (T2) et la bactérie inconnue DQ817737 (T4). Dans des conditions optimisées (aération, pH) le consortium bactérien MMP1 a permis de décolorer l'eau usée synthétique contenant de la mélanoidine à 18,3% en 2 jours. La comparaison de la décoloration par le consortium MMP1 avec un milieu abiotique a démontré que la décoloration était principalement due à l'activité biotique des cellules bactériennes, sans aucun phénomène d'adsorption. Un complément en minéraux et vitamines B n'a pas amélioré la décoloration de mélanoïdines avec le consortium bactérien MMP1. Enfin, les performances d'un bioréacteur à membrane pour traiter les eaux résiduaires synthétiques contenant de la mélanoïdine ont été évaluées à l’échelle du laboratoire. L'ensemencement du réacteur a été réalisé avec un inoculum sur la base du consortium MMP1. Le réacteur a fonctionné sous plusieurs conditions de temps de séjour hydrauliques (HRT) de 15, 20, et 40 heures. Les performances ont été analysées en termes de DCO (demande chimique en oxygène), décoloration et croissance de biomasse. Les résultats ont indiqué qu’une efficacité accrue d’élimination de la DCO et de la couleur ont été obtenues avec le HRT le plus longDistillery effluent from sugarcane molasses leads to an environmental pollution due to its large volume and the presence of dark brown colored compounds, known as melanoidins. In this study, a bacterial consortium CONS8 isolated from waterfall sediments in Maehongsorn province was selected as a molasses-decolorizing consortium. Consortium CONS8 was able to decolorize, only within 2 days, in Erlenmeyer flasks, three different synthetic wastewaters containing either Viandox sauce (13.5% v/v), beet molasses wastewater (41.5% v/v) or sugarcane molasses wastewater (20% v/v) at 9.5, 8.0 and 17.5%, respectively. Four predominant bacteria present in the consortium CONS8 were identified by the 16S rDNA analysis. To achieve the highest decolorization, the artificial bacterial consortium MMP1 comprising Klebsiella oxytoca, Serratia mercescens (T2) and unknown bacterium DQ817737 (T4), was constructed. Under optimized conditions (aeration, pH), the bacterial consortium MMP1 was able to decolorize the synthetic melanoidins-containing wastewater at 18.3% within 2 days. The comparison of decolorization by the consortium MMP1 with abiotic control proved that the color removal for synthetic melanoidins-containing wastewater medium was mainly due to biotic activity of bacterial cells, without any adsorption phenomena. Supplement of nutrients and vitamin B did not promote melanoidins decolorization by bacterial consortium MMP1. Finally, the performance of a membrane bioreactor (MBR) for synthetic melanoidins-containing wastewater treatment was investigated at laboratory scale, with a mineral membrane. The reactor seeding was made with the MMP1 bacterial consortium inoculum. The reactor was performed with several hydraulic retention times (HRT) of 15, 20, and 40 hours. The performances were analyzed in terms of COD, color removal and biomass in the reactor. The results indicated that the higher COD and color removal efficiency were achieved with the longer HRT
22
Bedi, Shalu, e Kiran Sharma. "Library Consortia: A Step forward the Information Society". Twenty First Century Publications, Patiala, 2008. http://hdl.handle.net/10150/105994.
Abstract (sommario):
The increasing price of electronic journals, indexing and abstracting databases along with the traditional published print subscriptions has forced library community to explore alternative means of subscription. The emergence of library a very promising development in this direction. The Phenomenon of consortia or group of libraries maintaining information resources together has become very common these days, In India, during last few years we witnessed many consortia based subscription. This paper briefly discusses the concept, need, advantages and also the major consortia initiatives in India.
23
Bidgood, Lee. "Consort Cooperative Concert". Digital Commons @ East Tennessee State University, 2017. https://vimeo.com/239922609.
24
VOLPATO, SILVIA. "Controllo qualità dei microrganismi". Doctoral thesis, Università degli studi di Modena e Reggio Emilia, 2020. http://hdl.handle.net/11380/1201052.
Abstract (sommario):
L'agricoltura simbiotica è un nuovo processo di coltivazione che prevede l’utilizzo di microrganismi benefici, come funghi, batteri e lieviti che rendono la rizosfera (lo spazio da 1 a 3 millimetri che avvolge le radici delle piante), estremamente ricca di micro-organismi cosiddetti “buoni” o funzionali. Tali microorganismi costituiscono il biota microbico che oltre a fungere da supporto per il benessere delle piante che li ospitano può contribuire all’azione antagonista nei confronti di microrganismi potenzialmente patogeni.
Numerosi prodotti a base microbica sono disponibili sul mercato: essi sono commercializzati come biofertilizzanti, biostimolanti, biopesticidi, promotori della crescita, induttori di resistenza, ecc… Pertanto, l’obiettivo della ricerca è quello di stabilire delle regole, delle procedure e dei protocolli da usarsi durante la produzione industriale di tali bioformulati, in modo da ottenere una formulazione microbica la cui qualità sia controllata durante il processo produttivo. Solamente operando un controllo di qualità oggettivo e preciso è possibile ottenere inoculi microbici efficienti, efficaci e sostenibili, tali che possano essere una valida alternativa ai pesticidi chimici.
Partendo da un prodotto biostimolante commerciale molto noto, Micosat F UNO, costituito da un consorzio microbiologico, la ricerca è stata orientata allo sviluppato un "protocollo di qualità" considerando sia i singoli microrganismi presenti sia il consorzio nel suo insieme.
I singoli componenti sono stati selezionali, accuratamente identificati, studiati e caratterizzati considerando le interazioni microrganismo-microrganismo e pianta-microrganismo. Di un microrganismo, in particolare, è stato sequenziato l’intero genoma e studiate le possibili vie biosintetiche al fine di dare supporto scientifico al suo ruolo di microrganismo benefico o funzionale e facilitarne il suo brevetto e inquadramento normativo come sostanza attiva. Infine è stato analizzato il processo produttivo ed identificati i punti di controllo al fine di ottenere un prodotto commerciale qualitativamente valido.Symbiotic agriculture is a new cultivation process that involves the use of microbial inoculants, such as fungi, bacteria and yeasts that make the rhizosphere (the space from 1 to 3 millimeters that envelops the roots of plants), extremely rich in beneficial microorganisms. These microorganisms constitute the microbial biota which, in addition to acting as a support for the well-being of the plants that host them, can contribute to the antagonistic action against potentially pathogenic microorganisms. Several products based on microorganisms are available on the market: they are advertised as biofertilisers, biostimulants, biopesticides, growth enancers, resistance inducers, etc... Therefore, the aim of the research is to set up rules, procedures and protocols to be used during the industrial production of microbials, in order to obtain an efficient, effective and sustainable microbial formulation, whose quality is checked along the production chain. Only by performing quality control checks, microbials can be a valid alternative to chemical pesticides. Research started from a well known, commercial biosimulant, namedMicosat F UNO, consisting of a microbiological consortium. Research was oriented towards the development of a "quality protocol" considering both the individual microorganisms present in the product and the consortium as a whole. The individual components were selected, thoroughly identified, studied and characterized considering their microbe-microbe and plant-microbe interactions. Full genome sequence and biochemical pathways were done and characterised for one particular benficial organism, in order to scietifically support its role as beneficial organisms and as possible, patentable active substance. Additionally, the production process was analyzed and control points were identified in order to obtain a qualitatively valid commercial product.
25
Pfannes, Kristina. "Characterization of the symbiotic bacterial partners in phototrophic consortia". Diss., lmu, 2007. http://nbn-resolving.de/urn:nbn:de:bvb:19-77693.
26
Laramée, Louise. "Genetic characterization of a diclofop-methyl-degrading bacterial consortium". Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp04/mq29733.pdf.
27
Santos, Paulo Ricardo Alves dos. "Corn consortium with forage: attributes physical soil and productivity". Universidade Federal do CearÃ, 2016. http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=17324.
Abstract (sommario):
Conselho Nacional de Desenvolvimento CientÃfico e TecnolÃgicoThe intensification of agricultural production systems is increasing in the current agriculture. In this sense, the simultaneous planting forage to grain crops, constitutes an alternative in the intensification of the production system, which can increase or not the productivity of the main crop, and produce straw for mulching. In order to check the occurrence of changes in the physical properties of the soil and in corn yield and dry matter production due to the corn consortium / forage in two sowing dates, this study was conducted in the experimental area of the Department of Agricultural Engineering of the Federal University of CearÃ. The design was used in a randomized block design in a factorial scheme (3x2) + 1 with four replications, totaling 28 experimental units. The treatments consisted of three forages: Brachiaria, Panicum maximum cv. MombaÃa and Crotalaria spectabilis intercropped with maize in two of fodder sowing dates, between the lines of simultaneous sowing maize (season 1 - E1) and corn leading the V4 stage of corn (season 2 - E2), and the control. The results showed that intercropping maize / forage did not interfere in phytotechnical characteristics of corn, nor in productivity, however produced changes in soil physical properties when the day of sowing in season 1. Despite the changes that have occurred in the soil, they were not sufficient to enhance the productivity of maize, which could possibly be related assessments on only one crop cycle. But when the goal was the production of dry straw, it is concluded that fodder Brachiaria brizantha and Mombasa at the time 1 (E1) are recommended
A intensificaÃÃo dos sistemas de produÃÃo agrÃcola à cada vez maior na atual agricultura. Nesse sentido, o plantio simultÃneo de forrageiras com culturas produtoras de grÃos, constitui em uma alternativa na intensificaÃÃo do sistema de produÃÃo, que poderà incrementar ou nÃo a produtividade da cultura principal, alÃm de produzir palha para cobertura do solo. Com o objetivo de verificar a ocorrÃncia de mudanÃas nas propriedades fÃsicas do solo, bem como na produtividade do milho e produÃÃo de matÃria seca em funÃÃo do consÃrcio milho/forrageiras em duas Ãpocas de semeadura, o presente trabalho foi conduzido na Ãrea experimental do Departamento de Engenharia AgrÃcola da Universidade Federal do CearÃ. Foi utilizado o delineamento em blocos ao acaso, no esquema fatorial (3x2) + 1 com quatro repetiÃÃes, totalizando 28 unidades experimentais. Os tratamentos foram constituÃdos por trÃs forrageiras: Brachiaria brizantha, Panicum maximum cv. MombaÃa e CrotalÃria spectabilis consorciadas com o milho em duas Ãpocas de semeadura das forrageiras, na entrelinha do milho simultÃneo a semeadura (Ãpoca 1 â E1) e na entrelinha do milho no estÃdio V4 do milho (Ãpoca 2 â E2), alÃm da testemunha. Os resultados permitiram concluir que a consorciaÃÃo milho/forrageiras nÃo interferiram nas caracterÃsticas fitotÃcnicas do milho, nem tampouco, na produtividade, porÃm proporcionaram modificaÃÃes nas propriedades fÃsicas do solo quando da realizaÃÃo da semeadura na Ãpoca 1. Apesar das modificaÃÃes ocorridas no solo, as mesmas nÃo foram suficientes em incrementar a produtividade do milho, o que possivelmente pode estar relacionado as avaliaÃÃes em apenas um ciclo da cultura. Jà quando o objetivo foi a produÃÃo de matÃria seca de palha, conclui-se que as forrageiras Brachiaria Brizantha e MombaÃa na Ãpoca 1 (E1) sÃo recomendadas
28
Claverie, Bruno. "Le consortium international et la décomposition des processus productifs". Paris 1, 1989. http://www.theses.fr/1989PA010014.
Abstract (sommario):
Le consortium international decompose les processus productifs de ses firmes partenaires, afin de mettre en oeuvre un projet commun. La permanence de la decomposition des processus productifs au sein du consortium le singularise dans l'ensemble des organisations conationales. Les consortiums sont un facteur du developpement de la decomposition des processus productifs. La decomposition des processus productifs est un facteur de competitivite du consortium international.
29
Keeling, Tom. "Architecture and Human Event: a Theatre for the Consortium". Thesis, Virginia Tech, 1999. http://hdl.handle.net/10919/45206.
Abstract (sommario):
Architecture is an interaction, a marriage between human event, human need, human spirit and built form. Architects range along the line of this interaction from those that embrace the complexity of humanity and struggle with form to those that embrace only form and cause humanity to struggle with the results.
Is it possible to stand in the center of the creative tension of this struggle, embracing and recognizing the power of built form to support, shelter, enliven, confront, uplift and even bring transformation, healing and poetic transcendence to the human event and therefore to life while simultaneously embracing the rich complexity, contradiction and paradox of human event which informs and interacts with the place of happening; to recognize and wed the power of both.
This thesis is an exploration which questions the relationship of human event and the architectural response to that event. Perhaps it may serve to stimulate discussion of the vital bond between human beings and the places they design for themselves to inhabit.Master of Architecture
30
Loria, Mark Henry II. "Microalgal-Bacterial Consortia for Biofuel Production and Wastewater Treatment". Case Western Reserve University School of Graduate Studies / OhioLINK, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=case1510234025755737.
31
Majumder, Apurba Jyoti. "Role of Consortia on Library and Information Science Education". Allied Publisher, 2007. http://hdl.handle.net/10150/105227.
Abstract (sommario):
The way and pace at which information is generated, organized and used is
witnessed rapid strides in recent times. Hence, the discipline of LIS meant to
manage and provide information service may not be taught effectively and practiced
perfectly through a framed curriculum in the formal education steam alone. Driven
by the market demands and user needs, the discipline is embracing other disciplines
like computers, communication technology, cognition research etc. to continuously
monitor and augment their skills to arrive at â pinpointed information from the
delugeâ . Manual means of tackling information will not help the user/professional to
solve emerging problems in the actual research setup and also the present day
researchers expect a faster response to their information needs. Information
management and servicing in a highly matured and skill intensive activity and it
requires people with different educational backgrounds. Electronic access is
increasingly providing a large proportion of current information instead of print and
allowing access through a variety of platforms on a twenty-four hour basis. As the
traditional custodians of information, librarians need to be aware of the implications
of these changes and develop technological and managerial skills that will enable
them to make effective use of information to meet their organization and changing
needs. However, many librarians lack confidence to learn and master the skills
required in adopting the increasingly sophisticated technology. It is vital that they
must be kept in touch with modern developments and maintains a proactive
approach to work in an ever-changing information world. Professional profiles are
changing so rapidly and very radically these days impacting the librarianâ s portfolio,
since libraries are becoming knowledge management organizations with librarians
as their active agents. Perhaps the most important development of libraries during
the current decade has been the move from organizational self-sufficiency to a
collaborative survival mode as personified by the growth of library consortia.
Information technology is now a level of cooperation that is much broader and deeper
than ever before
32
Sipes, Susan Diane. "ONE CONSORTIUM, TWO POPULATIONS: WHAT IT TAKES TO SUCCEED". OpenSIUC, 2010. https://opensiuc.lib.siu.edu/theses/324.
Abstract (sommario):
AN ABSTRACT OF THESIS OF Susan D. Sipes, for the Masters of Science degree in Workforce Education and Development, presented on October 11, 2010, at Southern Illinois University Carbondale. TITLE: ONE CONSORTIUM, TWO POPULATIONS: WHAT IT TAKES TO SUCCEED MAJOR PROFESSORS: Dr. Marcia Anderson and Dr. Beth Winfrey-Freeburg University consortia have existed for over 50 years as a practical solution to increase regional access to higher education and control costs. The challenge has been to determine how to measure the effectiveness of consortia in order to predict their success. The goal was to apply a business case to UCLC, treating students as customers, and administrators as business owners. Throughout the literature, much has been written to define the critical operational characteristics of successful consortia, but little has been done to explore the needs of consortia's customers. This work presents a compilation of operational characteristics with services desired by students in order to predict the success of one newer consortium, the University Center of Lake County in IL (UCLC). The characteristics and services of existing successful consortia were gleaned from the literature and a written survey instrument was developed. Students and administrators at UCLC were surveyed; their responses were compared to data extracted from other research using descriptive statistics and a chi-squared test. Results showed that UCLC exhibits many of the most important operational characteristics to be successful. Practical recommendations are provided to UCLC to further explore how to improve student services. For example, UCLC needs to increase its marketing and advertising efforts. Other consortia will be able to repeat the surveys to learn more about their operations and service levels.
33
Terkildsen, Sheryl Ramona. "End of life nursing education consortium grant implementation project". CSUSB ScholarWorks, 2002. https://scholarworks.lib.csusb.edu/etd-project/2324.
Abstract (sommario):
This project addressed the continuing education needs of nursing staff and other health care professionals for delivering competent and compassionate palliative or end of life care. The scope of the project included, writing a grant application, training and certification by the end of life Nursing Education Consortium and implementing an education program for staff at the Loma Linda Veterans Affairs medical center.
34
Fargier, Nathalie. "Consortium de bibliothèques et acquisition de périodiques électroniques : l'exemple de la bibliothèque de l'Université de Yale et du North East Research Libraries Consortium /". Villeurbanne : ENSSIB, 2001. http://www.enssib.fr/bibliotheque/documents/dcb/fargier.pdf.
35
Rosin, Artemino Raimundo. "A indústria do consórcio : considerações a respeito da atuação dos bancos no setor". reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2006. http://hdl.handle.net/10183/8797.
Abstract (sommario):
Este estudo analisa a indústria de consórcio a partir da prestação desse serviço pelos bancos de varejo, buscando compreender as transformações mais relevantes que ocorreram a partir de então. Para tanto, analisa-se o produto consórcio: sua origem e evolução, seu funcionamento, sua particularidade como forma de aquisição de bens, seu atrativo. É feita também a comparação do consórcio com outras formas de aquisição. Como forma de justificar o interesse dos bancos de varejo em administrar e constituir grupos de consórcio fez-se uma revisão na história recente do Sistema Financeiro Nacional com destaque para a importância das receitas de prestação de serviços numa economia com preços estabilizados. Para a análise da indústria de consórcio buscou-se, com base na participação do mercado, em seus vários segmentos autorizados, explicação para as modificações e o comportamento do setor, a partir do ingresso dos bancos de varejo.This study analyzes the industry of consortium since banks started retailing it. It tries to understand the most relevant transformations that have taken place since that day. In order to do so, the following aspects of the product consortium are analyzed: its origin and evolution, the way it works, its peculiarities as a way to acquire goods and its attractiveness. The consortium is also compared to other modes of good acquisition. In an attempt to justify the interest of retail banks in managing and assembling consortia, the recent history of the National Financial System of Brazil is reviewed. This review highlights the importance of the income which is a result of services offered in a stabilized price-economy. The changes in behavior and the modifications in the consortium industry caused by the joining of the retail banks in this business are analyzed on the base of the participation of these institutions in this particular industry.
36
Douet, Marie. "Les Consortiums maritimes de lignes régulières /". Caen (13, Brd du Maréchal Juin, 14000) : Paradigme, 1985. http://catalogue.bnf.fr/ark:/12148/cb37169228v.
Abstract (sommario):
Texte abrégé de: Thèse--Droit--Paris I, 1984.Bibliogr. p. 187-188. Thèse soutenue sous le titre : Effets structurants des consortiums sur le marché des lignes régulières : l'exemple des consortiums à participation française"
37
Douet, Marie. "Effets structurants des consortiums maritimes sur le marché des lignes régulières l'exemple des consortiums à participation française". Lille 3 : ANRT, 1985. http://catalogue.bnf.fr/ark:/12148/cb37594168x.
38
Dennis, Philip Christopher. "Community analysis of an anaerobic tetrachloroethene, PCE, degrading bacterial consortium". Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2000. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape2/PQDD_0019/MQ53371.pdf.
39
Nesin, Taunya W. "The Role of Research Consortia through the Eyes of Principals". Thesis, The George Washington University, 2014. http://pqdtopen.proquest.com/#viewpdf?dispub=3634158.
Abstract (sommario):
The Role of Research Consortia through the Eyes of Principals No Child Left Behind legislation calls for educational leaders to use evidence to inform practice. Principals are charged with improving student achievement by adjusting resources and policies based on most recent evidence and research available to them. A research consortium is an institution that partners with a school district to engage in ongoing research for the purpose of improving practice.
The purpose of this study was to understand principals' perceptions of research consortia associated with their district and to discover how principals used evidence produced by consortia. The overarching question of the study was "How do principals learn about and utilize evidence produced by research consortia associated with their district."
To address my research question, I interviewed 22 principals in two school districts that work with research consortia. The participants included 10 Baltimore City Public School principals and 12 Chicago Public School principals. The Baltimore Education Research Consortium (BERC) is associated with Baltimore City Public Schools. The University of Chicago Consortium on Chicago School Research (CCSR) is associated with Chicago Public Schools.
Using an interpretivist interview design, four major findings emerged from this study: (1) principals, who are aware of the evidence produced by research consortia, use it to inform practice and decision-making, (2) principals do not have many direct interactions with researchers in the consortia and do not see a pathway to connect with researchers in the consortia, (3) principals who knew about the research consortium in their district have positive perceptions of it, however some principals are skeptical of survey data regardless of the source, and (4) principals in this study explained that consortia do address the evidence to practice gap, but principals did not see an opportunity to contribute to research design.
40
Tucci, Christopher L. (Christopher Luigi). "Market, technical, and social overlap in technology collaborations and consortia". Thesis, Massachusetts Institute of Technology, 1997. http://hdl.handle.net/1721.1/10358.
41
Ghachem, Lassaad. "Étude de faisabilité d'un consortium entre les PME touristiques tunisiennes /". Thèse, Trois-Rivières : Université du Québec à Trois-Rivières, 2000. http://www.uqtr.ca/biblio/notice/resume/03-2224943R.html.
42
Solomons, Daniel D. "The consortium approach to employee assistance programs : a case study /". View abstract, 1999. http://library.ctstateu.edu/ccsu%5Ftheses/1549.html.
43
Ghachem, Lassaad. "Étude de faisabilité d'un consortium entre les PME touristiques tunisiennes". Thèse, Université du Québec à Trois-Rivières, 2000. http://depot-e.uqtr.ca/3161/1/000678031.pdf.
44
Williams, A. Lynn, Brenda Louw, Ken Bleile, Marcia Keske-Soares, Inge Trindade e Nancy J. Scherer. "US-Brazil cross-linguistic Consortium in Speech and Hearing Sciences". Digital Commons @ East Tennessee State University, 2011. https://dc.etsu.edu/etsu-works/2066.
45
Ray, Sayani. "Elucidation of enzymatic consortia for structural chemotyping of dicot hemicelluloses". Nantes, 2014. https://archive.bu.univ-nantes.fr/pollux/show/show?id=f8241303-d93f-45e4-a109-321bfc9281ff.
Abstract (sommario):
Xyloglucanes (XyG), galactoglucomannanes (GgM) et glucuronoarabinoxylanes (GAX) sont des hémicelluloses clés pour la régulation des fonctions de la paroi chez les dictoylédones. Cependant, l’impact de leur composition variée et de leur distribution dans la paroi reste à élucider. Pour explorer leur structure fine, la dégradation par des enzymes commerciales des fractions natives de XyG, GgM et GAX acetylés a révélé des structures différentes entre différentes populations de chaque hémicellulose, avec en particulier la première mise en évidence de substitutions arabinose et/ou xylose sur les GAX. Des détails structuraux restent toutefois à élucider, ce qui nécessite de nouveaux outils enzymatiques dotés de nouvelles spécificités. A cette fin, nous avons utilisé une démarche d’analyse du génome couplée à un criblage moyen-débit pour sélectionner des bactéries de diverses origines produisant des hémicellulases. Ainsi, 4 souches aérobies et non-pathogènes (Pseudoalteromonas atlantica, Cellulophaga algicola, Caulobacter crescentus, Paenibacillus sp. ) ont été cultivées dans différentes conditions. P. Atlantica s’est avéré le plus efficace pour produire des hémicellulases. Son sécrétome a été fractionné par diverses méthodes chromatographiques, ce qui a permis d’isoler des β- glucanase, β-xylanase, β-xylosidase and α- arabinofuranosidase. L’analyse des hydrolysats d’hémicelluloses de pomme par ces enzymes a révélé des spécificités de substrats diverses et l’analyse structurale des oligosaccharides a fourni de nouvelles avancées sur la structure de ces polysaccharidesXyloglucan (XyG), galactoglucomannan (GgM) and glucuronoarabinoxylan (GAX) are key hemicelluloses regulating plant cell wall functions in dicots. However, the impact of their diverse sugar composition and distribution in planta remains unclear. To detail their fine structure, degradation by commercial enzymes of purified native acetylated XyG, GgM and GAX fractions from apple revealed structural variations between different XyG and GgM populations and demonstrated first evidence for arabinose and/or xylose substitution on GAX. However, more detailed fine structural aspects of these polysaccharides require new enzymatic tools with regard to cleaving site specificities. In this aim, we used a genome mining approach coupled with a medium- through put screening procedure to identify hemicellulose-degrading enzymes from marine and land bacteria. Based on the presence of potential hemicellulases in their annotated genome, 4 culturable aerobic and nonpathogenic bacterial strains from marine (Pseudoalteromonas atlantica, Cellulophaga algicola), oligotropic (Caulobacter crescentus) and terrestrial (Paenibacillus sp. ) environment were selected. Of the 4 strains, P. Altantica was chosen for its culture supernatant appreciable activity towards fruit hemicelluloses. The secretomes were fractionated by ion-exchange chromatography to isolate β-glucanase, β- xylanase, β-xylosidase and α-arabinofuranosidase. The analysis of hydrolyzates revealed different substrate specificities, which together with structural analyses of isolated oligosaccharides provided novel insights of apple hemicelluloses complexity
46
Dessemontet, Raphaël. "La consortium de construction et sa fin prématurée en droit suisse /". Lausanne : Centre de Droit de l'Entreprise de l'Univ. de Lausanne, 2006. http://www.gbv.de/dms/spk/sbb/recht/toc/514860839.pdf.
47
Alzahrany, Hashim. "Hydrocarbon remediation by microbial consortium : validation with molecular and biotechnological tools". Thesis, University of Aberdeen, 2012. http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=185862.
Abstract (sommario):
There is a need for sustainable approaches in the remediation of hydrocarbon (HC)-impacted environments. Bioremediation has gained prominence but to be effective this requires consideration of the physical, chemical and biological processes in an environmental matrix. In the first part of this study, physical constraints to effective bioremediation of drill cutting (DC) were quantified using slurry-phase treatments. The DC:water ratio and aeration of the DC slurries were optimised. Results indicated that physical parameters, unless effectively managed, could greatly influence the performance of bioremediation campaign, both in terms of end-point and duration. Once physical aspects have been addressed, it is important to understand both chemical and biological processes. There has been a great deal of work considering the significance of chemical processes such as bioavailability, pH performance range and nutrient optimisation. These were not investigated in this programme of research. Bioaugmentation (microbial seeding of contaminated soils) has been proposed as a significant method for bioremediating HC-contaminated matrices. However, the value of bioaugmentation has been the subject of considerable controversy. The performance of bioaugmentation was studied in this project both directly and indirectly. Compounds capable of encouraging the activity of HC degraders were added to media. This "conditioning" process was investigated and quantified in three major experiments. Firstly, the conditioning of a HC-degrading bacterial consortium using selective enrichment substrates was studied. Here, INT reduction was monitored over time using 96-well microplates containing mineral media supplemented with diesel, toluene, hexadecane and phenanthrene. The colour development resulting from conditioning on different substrates varied significantly (P≤ 0.05). Secondly, the performance of HC degraders (Pseudomonas putida F1 and it's bioluminescent derivative, P. putida TVA8) after conditioning on a range of catabolic inducer substrates was studied. The development of an optimised experimental procedure to study carbon transformation of toluene as a consequence of conditioning was reported. Cultures conditioned on toluene had significantly (P≤ 0.05) higher activity, biomass and toluene degradation rates than the other treatments. Finally, the effect of conditioning three HC-degrading bacteria (Pseudomonas putida NCIMB 9816, Pseudomonas sp. and Klebsiella sp.), individually and in combination, on degradative performance and relative species abundance was assessed. DGGE profiles indicated significant (P≤ 0.05) changes in relative species abundance but, generally, there were no significant differences in HC degradation or cumulative respiration. The results reported in this thesis, at the genotypic, phenotypic and functional levels reveal the complexity of HC remediation and the need to combine detailed analytical chemistry with focussed microbial measurements.
48
Jeanbille, Mathilde. "Réponse des consortia microbiens benthiques à une contamination chronique aux hydrocarbures". Thesis, Pau, 2015. http://www.theses.fr/2015PAUU3043/document.
Abstract (sommario):
Les communautés microbiennes procèdent au recyclage des nutriments et à la degradation de la matière organique, et sont ainsi essentielles aux cycles biogéochimiques dans le sédiment et plus largement dans les océans. La contamination chronique aux hydrocarbures représente près de 80% des déversements totaux dans les océans. Toutefois, en comparaison des marées noires, son impact sur les communautés microbiennes est encore mal compris. Dans cette étude, nous avons d’abord utilisé une approche de type méta-analyse pour élucider l’effet global de la contamination aux hydrocarbures dans différents habitats. La réponse des communautés bactériennes à la contamination s’est révélée être dépendante du type d’habitat, les sols étant plus impactés que d’autres habitats, comme par exemple les sédiments marins. Nous nous sommes ensuite intéressés aux communautés microbiennes des trois domaines du vivant de sédiments côtiers provenant des côtes méditerranéennes et atlantiques. La contamination chronique n’influençait que marginallement les communautés benthiques, et la diversité alpha n’était pas réduite dans les sédiments contaminés. Cedendant, la comparaison des réseaux de co-occurrence des échantillons contaminés et non-contaminés a montré que le réseau des communautés contaminées présentait une topologie différente, indiquant une vulnérabilité plus importante à d’éventuelles perturbations environnementales. Des indicateurs potentiels de la contamination identifiés avec la méta-analyse ont été ciblés pour étudier l’impact de la contamination chronique aux hydrocarbures sur les services écologiques qu’ils assurent (i.e. la dégradation de la matière organique et des hydrocarbures) en utillisant la technique de Micro-FISHWithin the sediment, microbial communities play a pivotal role by driving essential processes such as nutrient cycling and organic matter degradation. Chronic hydrocarbons contamination represents almost 80% of the total input in the oceans. However, as compared to oil spills, its impact on microbial communities remains poorly understood. In this study, we first used a meta-analysis approach to decipher the global effect of hydrocarbons contamination in different habitats. Bacterial community response to the contamination was found to be dependant of the habitat studied, with soils being more impacted than other habitats, like marine sediments. Because bacteria are in interactions with other important members of microbial communities such as Archaea and Eukaryotes, we focused on microbial communities from the three domains of life in coastal marine sediments from the Mediterrranean and the French Atlantic coasts. Independently of the domains of life, chronic hydrocarbons contamination appeared to be a poor driver of communities structuration, and alpha diversity was not reduced in contaminated sediments. However, the comparison of co-occurences networks of contaminated and non-contaminated samples showed that the network from the contaminated samples exhibited a different topology, which suggests a higher vulnerability to eventual environmental perturbations. Potential indicators species identified using the meta-analysis approach were targeted to study the impact of chronic contamination on the ecological services they provide (i.e. organic matter and hydrocarbons degradation) using the Micro-FISH method
49
Ortiz, Onofre. "Degradation studies of 2, 4, 6, trinitrotoluene by a microbial consortia". DigitalCommons@Robert W. Woodruff Library, Atlanta University Center, 1997. http://digitalcommons.auctr.edu/dissertations/3269.
Abstract (sommario):
Microbial mats are natural heterotrophic and autotrophic communities dominated by cyanobacteria (blue-green algae). These constructed mats are durable, tolerant to a variety of toxins and resilient under changing environmental conditions. This research demonstrates that microbial mats provide an effective remediation treatment for 2,4,6 Trinitrotoluene (TNT) in water and soil. It showed that TNT is removed to undetectable limits after 5 days of treatment under any of the following conditions: light/dark; total light; total dark. This work also shows that in the presence of an inorganic material (lead), mats were able to remove both contaminants efficiently, thus making the microbial mat a good choice for mixed waste remediation. Kinetic studies performed during the first five hours of microbial treatment showed a pseudo first order reaction indicating that TNT removal is initially proportional to the concentration of TNT. The major metabolites detected after 24 hour of treatment were 4-amino-2,6- dinitrotoluene, 2-amino-4,6-dinitrotoluene, and 2,4-diamine-6-nitrotoluene. These metabolites have a toxicity level similar to TNT. However, mat extracts and growth medium concentrates taken after 24 hours treatment of TNT showed little or no toxicity. The lack of toxicity demonstrated by treated mat extracts and media concentrates suggest that these metabolites are not the final metabolic products. The chemical nature of these metabolites suggests that the chemical mechanism of biotransformation involves reduction of the nitro groups at the ortho and para position of the TNT structure. Results obtained from light and dark experiment suggest that photooxidation or photodegradation is not an important mechanism for degradation of TNT by mats. Results show that live mats likely degrade TNT via a biotransformation process. In comparison, heat killed mats show a much slower removal of TNT than live mats. TNT was the only species found in the water column and extracts of heat killed mats, which indicates that TNT is removed by a passive absorption process, but no evidence of biodegradation was observed.
50
Yunita, Dewi. "The role of non-starter bacterial consortia in mould-ripened cheese". Thesis, University of Nottingham, 2016. http://eprints.nottingham.ac.uk/33010/.
Abstract (sommario):
Stichelton is a blue-veined raw milk cheese which is made following the Stilton cheese making process. In a previous study, a preliminary examination of its microflora during production was examined by traditional culture methods and initial PCR DGGE profiling. The aim of this study was to complete the profiling of Stichelton cheese and examine the contribution of its microbiota components to product characteristics. Stored samples of cheese production isolates were sequenced and in addition whole population DNA was extracted directly from a fully ripened Stichelton cheese (12 weeks) and from bulk cell suspensions collected on various media. The V3, V4V5 and V6V8 regions of 16S rDNA were amplified by PCR and separated by DGGE using 20 – 80 % urea formamide denaturing gradient. While Lactobacillus casei/paracasei, Staphylococcus equorum, Bacillus sp., Brevibacterium sp., Halomonas sp., Acinetobacter sp., Alkalibacterium sp. and Corynebacterium casei were only found by the molecular method, traditional culture detected a large number of potentially raw milk microbiota. Lactococcus lactis was detected in the raw milk sample and along the process by both methods. The L. lactis subsp. lactis which was detected in the core of matured Stichelton was shown by PFGE to be from the raw milk and not the starter culture used in Stichelton production. S. equorum was found in the crust of cheese pre-piercing and in all parts of the cheese post-piercing by the molecular approach only. This suggested this organism was introduced originally via handling. Five S. equorum isolated from Stilton, Danish Blue and Reblochon could grow up to 10% salt but did not tolerate low pH levels suggesting S. equorum in Stichelton must have been introduced by handling before or during ripening as if it was present in the early fermentation then it would die as fermentation progressed due to pH sensitivity. A model cheese system made with commercial UHT milk was used to examine the interaction between mixed Lc. lactis, P. roqueforti and S. equorum B2 isolated from the Stilton crust. S. equorum was either added 1.5 h after the addition of L. lactis or it was smeared on the surface of the cheese immediately after un-moulding. The viable counts and pH were analysed throughout the process, while texture, water activity and flavour volatiles using GCMS SPME were determined for one month ripened cheeses only. The results showed S. equorum survived in the cheese following either method of introduction and that in cheeses without P. roqueforti addition, the presence of incorporated and surface-spread S. equorum could inhibit the surface growth of a contaminant Penicillium. It also slowed the growth of starter P. roqueforti in cheeses made with this mould. A paler coloured crust, firmer textured cheese and a low amount of alcohols were shown in the model cheeses made with surface-smear S. equorum. Conversely, addition of S. equorum in the initial process made the cheese core softer and produced low amounts of acids. Ethanol, 3-methyl-1-butanol and 2-pentanone were the main flavour compounds in the model cheeses examined. The antifungal activity of the isolate was confirmed in laboratory media. Its ability to prevent Penicillium surface growth could be beneficial for white cheeses where this is an undesirable flaw. The results showed that the sporulation inhibitory effect on P. roqueforti was because of an antifungal agent produced by S. equorum, but it was not acid, bacteriocin or H2O2. Further study is needed to detect the antifungal agent. Overall, the study has expanded the understanding of the role non-starter bacteria may have in contributing to cheese ripening.