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Letteratura scientifica selezionata sul tema "Confocal microscopy"

Autore: Grafiati

Pubblicato: 4 giugno 2021

Ultima modifica: 30 luglio 2024

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Articoli di riviste sul tema "Confocal microscopy"

J. H., Youngblom, Wilkinson J. e Youngblom J.J. "Telepresence Confocal Microscopy". Microscopy and Microanalysis 6, S2 (agosto 2000): 1164–65. http://dx.doi.org/10.1017/s1431927600038319.

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The advent of the Internet has allowed the development of remote access capabilities to a growing variety of microscopy systems. The Materials MicroCharacterization Collaboratory, for example, has developed an impressive facility that provides remote access to a number of highly sophisticated microscopy and microanalysis instruments. While certain types of microscopes, such as scanning electron microscopes, transmission electron microscopes, scanning probe microscopes, and others have already been established for telepresence microscopy, no one has yet reported on the development of similar capabilities for the confocal laser scanning microscope.At California State University-Stanislaus, home of the CSUPERB (California State University Program for Education and Research in Biotechnology) Confocal Microscope Core Facility, we have established a remote access confocal laser scanning microscope facility that allows users with virtually any type of computer platform to connect to our system. Our Leica TCS NT confocal system, with an interchangeable upright (DMRXE) and inverted microscope (DMIRBE) set up,

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Youngblom, J. H., J. Wilkinson e J. J. Youngblom. "Telepresence Confocal Microscopy". Microscopy Today 8, n. 10 (dicembre 2000): 20–21. http://dx.doi.org/10.1017/s1551929500054146.

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Jester, J. V., H. D. Cavanagh e M. A. Lemp. "In vivo confocal imaging of the eye using tandem scanning confocal microscopy (TSCM)". Proceedings, annual meeting, Electron Microscopy Society of America 46 (1988): 56–57. http://dx.doi.org/10.1017/s0424820100102365.

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New developments in optical microscopy involving confocal imaging are now becoming available which dramatically increase resolution, contrast and depth of focus by optically sectioning through structures. The transparency of the anterior ocular structures, cornea and lens, make microscopic visualization and optical sectioning of the living intact eye an interesting possibility. Of the confocal microscopes available, the Tandem Scanning Reflected Light Microscope (referred to here as the Tandem Scanning Confocal Microscope), developed by Professors Petran and Hadravsky at Charles University in Pilzen, Czechoslovakia, permits real-time image acquisition and analysis facilitating in vivo studies of ocular structures.Currently, TSCM imaging is most successful for the cornea. The corneal epithelium, stroma, and endothelium have been studied in vivo and photographed in situ. Confocal scanning images of the superficial epithelium, similar to those obtained by scanning electron microscopy, show both light and dark surface epithelial cells.

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Youngblom, J. H., J. Wilkinson e J. J. Youngblom. "Confocal Laser Scanning Microscopy By Remote Access". Microscopy Today 7, n. 7 (settembre 1999): 32–33. http://dx.doi.org/10.1017/s1551929500064798.

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In recent years there have been a growing number of facilities interested in developing remote access capabilities to a variety of microscopy systems. While certain types of microscopes, such as electron microscopes and scanning probe microscopes have been well established for telepresence microscopy, no one has yet reported on the development of similar capabilities for the confocal microscope.At California State University, home to the CSUPERB (California State University Program for Education and Research in Biotechnology) Confocal Microscope Core Facility, we have established a remote access confocal laser scanning microscope facility that allows users with virtually any type of computer platform to connect to our system.

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Stefani, Caroline, Adam Lacy-Hulbert e Thomas Skillman. "ConfocalVR: Immersive Visualization for Confocal Microscopy". Journal of Molecular Biology 430, n. 21 (ottobre 2018): 4028–35. http://dx.doi.org/10.1016/j.jmb.2018.06.035.

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Jason Kirk. "Beyond the Hype - Is 2-Photon Microscopy Right for You?" Microscopy Today 11, n. 2 (aprile 2003): 26–29. http://dx.doi.org/10.1017/s1551929500052469.

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Confocal microscopes have come a long way in the past decade. Not only are they more stable and easier to use than ever before, but their cost has dropped enough that multi-user facilities are finding competition from individual labs using the new breed of "personal" confocals. In fact it has, in some cases, become the de facto standard for fluorescence imaging regardless of whether the user actually has requirements for it or not.But, researchers always have an ear out for something better. Enter 2-photon microscopy (2PLSM). The “bigger & badder” cousin of the confocal microscope has become a new weapon in the arsenal of a microscopy industry that caters to researchers who can't wait to fill their labs with the latest and greatest imaging systems. Advertised by the industry and researchers alike as a technique that seems to solve most of the problems that plague confocal, “2-photon” has become the new buzzword in the vocabulary of researchers eager to enhance their fluorescence applications.

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Chapman, George B., e T. Wilson. "Confocal Microscopy". Transactions of the American Microscopical Society 110, n. 2 (aprile 1991): 194. http://dx.doi.org/10.2307/3226760.

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Wilson, T., e Barry R. Masters. "Confocal microscopy". Applied Optics 33, n. 4 (1 febbraio 1994): 565. http://dx.doi.org/10.1364/ao.33.000565.

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Beuerman, Roger W. "Confocal Microscopy". Cornea 14, n. 1 (gennaio 1995): 1???2. http://dx.doi.org/10.1097/00003226-199501000-00001.

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Lichtman, Jeff W. "Confocal Microscopy". Scientific American 271, n. 2 (agosto 1994): 40–45. http://dx.doi.org/10.1038/scientificamerican0894-40.

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Tesi sul tema "Confocal microscopy"

Naredi-Rainer, Nikolaus. "Advanced confocal microscopy". Diss., Ludwig-Maximilians-Universität München, 2014. http://nbn-resolving.de/urn:nbn:de:bvb:19-168349.

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Confocal microscopy is known for its capability to produce exceptional 3D images, even in living tissue. At the same time, it is a powerful spectroscopic tool, facilitating fluores- cence methods such as Fluorescence Correlation Spectroscopy (FCS) or single-molecule Förster Resonance Energy Transfer (FRET). It is heavily used to investigate a wide range of biological problems. This holds true especially for protein properties such as ligand binding, complex formation, conformational changes, or the intracellular distribution of the species in question. In this work, I will describe the assembly of two instruments: The first is a multi- parameter fluorescence detection (MFD) setup. It is a purely spectroscopic tool that offers the capability to characterize a fluorescent molecule, delivering information like fluorescence lifetime, anisotropy or the speed of its diffusion in free solution. When the molecule of interest is labelled with two fluorophores, additional information, like the energy transfer in-between them, becomes accessible and the correct distance between these two fluorophores can be calculated. If the two fluorophores are attached to different molecules, the MFD setup can detect interactions of these molecules in the range from pM up to μM with the help of Fluorescence Cross-Correlation Spectroscopy (FCCS). The second instrument, a stimulated emission depletion setup, combines some of the mentioned techniques, like FCS, with the superior image capability of a confocal micro- scope. One particular problem of fluorescent microscopes, though, is that image resolution is always restricted to the diffraction limit of the wavelength of the laser light. The STED setup utilizes the effect of stimulated emission in order to circumvent the diffraction bar- rier and allows images with a three-fold resolution increase, down to 75nm. These two setups will be used for several applications: The first will be centered around the molecular conformation of proteins, which are sensitive to the nature of the aqueous environment. In particular, the presence of ions can stabilize or destabilize (denature) protein secondary structure. The underlying mechanisms of these actions are still not fully understood. I will apply single-pair FRET to a small 29 amino acid long model peptide to investigate unfolding mechanisms of different unfolding reagents from the Hofmeister series, like sodium perchlorate or guanidinium chloride. The results show that certain salts, which are commonly summarized as denaturing agents, achieve the unfolding by either collapsing the molecule to a compressed state or swelling it to a denatured state. 7 The second application of the MFD setup is the investigation of the enhanced green fluorescent protein (EGFP). Although highly used in biochemistry and biophysics, for example to read out the expression level of genes, it is still not fully known what percentage of EGFP is fluorescent. This lack of knowledge makes it nearly impossible to make quantitative statements. With the help of FCCS, it is shown that the folding efficiencies range from 40 − 90%, depending on the environment of the fluorescent protein and which particular mutant is used. In the third application, the focus will be shifted to nucleation- and polymerization- behavior of actin. The actin cytoskeleton is a central mediator of cellular morphogenesis, and rapid actin reorganization drives essential processes such as cell migration and cell di- vision. In order to compare results of confocal spectroscopy methods with well-established bulk essays, we successfully ported the standard bulk essay to the confocal microscope, allowing for the first time to follow the decrease of monomer concentration and appear- ance of small filaments. Also, the formation of dimers or other small oligomers below the critical concentration is proven for the first time, using FCCS. The last application will utilize the STED setup in order to carry out the first steps towards the investigation of the nucleation and branching behavior of actin in cooperation with the actin related protein 2/3 (ARP2/3). This protein complex preferentially attaches to actin filaments that are located at the leading edge of a cell and forms branched filamentous structures. The exact conditions under which this process occurs are not well characterized. This part of the work will deal with the steps that are necessary to follow the polymerization process on the STED setup.

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Pacheco, Shaun, e Shaun Pacheco. "Array Confocal Microscopy". Diss., The University of Arizona, 2017. http://hdl.handle.net/10150/623252.

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Confocal microscopes utilize point illumination and pinhole detection to reject out-of-focus light. Because of the point illumination and detection pinhole, confocal microscopes typically utilize point scanning for imaging, which limits the overall acquisition speed. Due to the excellent optical sectioning capabilities of confocal microscopes, they are excellent tools for the study of three-dimensional objects at the microscopic scale. Fluorescence confocal microscopy is especially useful in biomedical imaging due to its high sensitivity and specificity. However, all designs for confocal microscopes must balance tradeoffs between the numerical aperture (NA), field of view (FOV), acquisition speed, and cost during the design process. In this dissertation, two different designs for an array confocal microscope are proposed to significantly increase the acquisition speed of confocal microscopes. An array confocal microscope scans an array of beams in the object plane to parallelize the confocal microscope to significantly reduce the acquisition time. If N beams are used in the array confocal microscope, the acquisition time is reduced by a factor of N. The first design scans an array of miniature objectives over the object plane to overcome the trade-off between FOV and NA. The array of objectives is laterally translated and each objective scans a small portion of the total FOV. Therefore, the number of objectives used in the array limits the FOV, and the FOV is increased without sacrificing NA. The second design utilizes a single objective with a high NA, large FOV, and large working distance designed specifically for whole brain imaging. This array confocal microscope is designed to speed up the acquisition time required for whole brain imaging. Utilizing an objective with a large FOV and scanning using multiple beams in the array significantly reduces the time required to image large three-dimensional volumes. Both array confocal microscope designs use beam-splitting gratings to efficiently split one laser beam into a number of equal energy outgoing beams, so this dissertation explores design methods and analyses of beam-splitting gratings to fabrication errors. In this dissertation, an optimization method to design single layer beam-splitting gratings with reduced sensitivity to fabrication errors is proposed. Beam-spitting gratings are typically only designed for a single wavelength, so achromatic beam-splitting grating doublets are also analyzed for possible use in array confocal microscopes with multiple excitation wavelengths. An analysis of the lateral shift between grating layers in the achromatic grating doublet proves grating profiles with constant first spatial derivatives are significantly less sensitive than continuous phase profiles. These achromatic grating doublets have designed performance at two wavelengths, but the diffraction angles at the two wavelengths differ. To overcome that limitation, scale-invariant achromatic gratings are designed, which not only provide designed performance at two wavelengths, but also equal diffraction angles at two wavelengths.

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Ye, Peng. "Compressive confocal microscopy". Access to citation, abstract and download form provided by ProQuest Information and Learning Company; downloadable PDF file, 50 p, 2009. http://proquest.umi.com/pqdweb?did=1889084501&sid=3&Fmt=2&clientId=8331&RQT=309&VName=PQD.

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Owen, Gabrielle M. "Coherence gated confocal microscopy". Thesis, Massachusetts Institute of Technology, 1993. http://hdl.handle.net/1721.1/12434.

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Thesis (B.S.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 1993.
Includes bibliographical references (leaves 33-34).
by Gabrielle M. Owen.
B.S.

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Sfalcin, Ravana Angelini 1985. "Avaliação de propriedades físico-químicas de infiltrantes experimentais com adição de partículas de vidro bioativas = Evaluation of the physical-chemical properties of experimental infiltrants incorporated with bioactive glass particles". [s.n.], 2015. http://repositorio.unicamp.br/jspui/handle/REPOSIP/288423.

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Orientador: Americo Bortolazzo Correr
Tese (doutorado) - Universidade Estadual de Campinas, Faculdade de Odontologia de Piracicaba
Made available in DSpace on 2018-08-27T03:22:53Z (GMT). No. of bitstreams: 1 Sfalcin_RavanaAngelini_D.pdf: 1513616 bytes, checksum: bd6dc4a4843283522343b56a58ef8ec7 (MD5) Previous issue date: 2015
Resumo: O objetivo neste trabalho foi avaliar as propriedades físico-químicas de infiltrantes resinosos com adição de partículas bioativas, bem como sua capacidade de penetração e dureza da profundidade em lesões subsuperficiais de esmalte. Uma blenda contendo TEGDMA (75% em peso) e BisEMA (25% em peso) foi manipulada e a partir dela foram incorporados 5 tipos de partículas bioativas (10% em peso): hidroxiapatita (HAp), fosfato de cálcio amorfo (ACP), vidro bioativo policarboxilato de zinco (BAG Zn), vidro bioativo 45S5 (BAG 45S5), cimento de silicato de cálcio modificado por ?-TCP (HCAT-?). Um material comercial foi utilizado (ICON®) como controle. Dez espécimes foram confeccionados para cada grupo de cada teste: rugosidade superficial (Ra) antes e após a escovação; Resistência à flexão por 3 pontos (RF) e módulo de elasticidade (ME); resistência coesiva à tração (RC); dureza Knoop (KHN); densidade de ligação cruzada (DLC); grau de conversão (GC); sorção (S) e solubilidade (SL) em água; e micro-dureza (KHN). Os dados foram submetidos a ANOVA e teste Tukey (?=0.05). A penetração dos infiltrantes resinosos no esmalte humano desmineralizado foi qualitativamente avaliada em Microscopia Confocal de Varredura a Laser (n=5). Os resultados mostraram que os menores valores de rugosidade (antes e após a escovação foram apresentados pelo ACP. Com relação à resistência a flexão e módulo de elasticidade, T+B apresentou o maior valor e ICON® mostrou o menor valor. ICON® também mostrou o menor valor de resistência coesiva à tração; não houve diferença significativa entre os grupos T+B, HAp, ACP, BAG Zn, BAG 45S5 e HCAT-?. Para o teste de dureza Knoop, ICON® obteve o menor valor e BAG Zn mostrou o maior valor. Para densidade de ligação cruzada, ICON® apresentou maior quantidade de ligação cruzada e HAp, menor quantidade de ligação cruzada. ICON® apresentou grau de conversão significantemente menor que os infiltrantes experimentais, que não diferiram entre eles. ICON® apresentou a maior sorção de água e HAp a menor. Não houve diferença significativa entre os demais grupos. Para solubilidade, ICON® apresentou os maiores valores, mas sem diferença de ACP. BAG 45S5 apresentou a menor solubilidade. Com relação a micro-dureza, não houve diferença estatisticamente significante entre as profundidades avaliadas (50 µm, 200 µm, 350 µm e 500 µm). BAG 45S5, BAG Zn e HCAT-? não mostraram diferença estatística entre eles. Entretanto, HCAT-? e BAG Zn foram similares ao ICON® e ACP. O grupo cariado mostrou menor valor quando comparado a todos os grupos testados. A análise em microscopia confocal mostrou que todos os materiais apresentaram boa capacidade de penetração nas lesões iniciais, exceto para FCA. Pôde ser concluído que adição de partículas bioativas em um infiltrante experimental melhorou as propriedades mecânicas e não afetou a capacidade de penetração dos infiltrantes. O infiltrante resinoso contendo fosfato de cálcio amorfo foi o que apresentou o melhor desempenho no teste de rugosidade de superfície antes e após a escovação
Abstract: The aim of this study was to evaluate the physical-chemical properties of the experimental infiltrants with the addition of bioactive particles as well as their capability of penetration and depth Knoop hardness into caries-like lesions. A control blend was made with TEGDMA (75 wt%) and BisEMA (25 wt%). Five bioactive fillers were added in the control blend (10 wt%): Hydroxyapatite (Hap), amorphous calcium phosphate (ACP), Zinc-polycarboxylated bioactive glass (BAG-Zn), bioactive glass 45S5 (BAG 45S5), and ?-TCP modified calcium silicate cements (HCAT-?). An available commercially material was used (ICON®). Ten specimens were comprised by each group for the following tests: Surface roughness (Ra) before and after brushing abrasion; flexural strength (FS) and elastic modulus (E-Modulus); tensile cohesive strength (TCS); Knoop hardness (KHN); softnening ratio (SR); degree of conversion (DC); water sorption (WS) and solubility (SL); and micro-hardness (micro-KHN). Data were subjected to ANOVA and Tukey¿s test (?=0.05). Confocal Scanning Laser Microscopy was used to evaluate qualitatively the penetration capability of resin infiltrants into demineralized human enamel. Results showed that ACP had the lowest Ra before and after brushing abrasion. Regarding to the FS and E-modulus, T+B showed the higher value and ICON® showed the lower value. Also, ICON® showed the lower value of TCS, but there was no significant statistically difference among the groups T+B, HAp, ACP, BAG Zn, BAG 45S5 e HCAT-?. To the KHN, ICON® obtained the lower value and BAG Zn showed the higher value. According to the SR, ICON® showed lower SR and HAp, the higher SR. ICON showed DC significantly lower than experimental resin infiltrants. Regarding to the WS, ICON® presented the highest water sorption and HAp the lowest one. There was no significant statistically difference among the other groups. ICON showed the highest SL results; however, the results were similar to ACP. The lowest SL was found for BAG 45S5. Regarding to the micro-KHN, there was no statistically difference among the analyzed depths (50 µm, 200 µm, 350 µm and 500 µm). BAG 45S5, BAG Zn and HCAT- ? did not show statistical difference among them. However, HCAT- ? and BAG Zn were similar to ICON® and ACP. Carious group showed lower value when compared to all the tested groups. Confocal microscopy analysis showed good capability of penetration into the initial lesions for all materials, except for ACP. It could be concluded that the addition of bioactive particles into an experimental infiltrant improved the mechanical properties and did not affect the capability of penetration into the experimental infiltrants. The resin infiltrant with amorphous calcium phosphate presented the best performance to the roughness surface before and after brushing abrasion
Doutorado
Materiais Dentarios
Doutora em Materiais Dentários

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Booth, Martin J. "Adaptive optics for confocal microscopy". Thesis, University of Oxford, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.393566.

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Carlini, A. R. "Imaging modes of confocal scanning microscopy". Thesis, University of Oxford, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.233485.

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Alawadhi, Fahimah. "Statistical image analysis and confocal microscopy". Thesis, University of Bath, 2001. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.341639.

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Lewin, Erland. "Approaches to Optimizing High Content Confocal Microscopy". Licentiate thesis, KTH, Applied Physics, 2009. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-10691.

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This thesis presents two methods for improving high contentconfocal microscopy.

The author's part in the first, "Toward a confocal subcellular atlasof the human proteome" was automating image capture of foursimultaneously tagged structures in cells in 96 well plates. In total,thousands of images of hundreds of proteins in cells. The authorwas also part of deciding which imaging methods should be used tomaximize image information content and quality, given the limitedtime available per well in order to scan large numbers of wells.

The second project, "Improved water permeability measurementsbased on fluorescence normalization" involves increasing the sensitivityof measurements of protein function by normalizing with anestimate of the amount of protein in the cell - the fluorescentsignal of a co-transfected protein. This could lead to achievingsufficient confidence in measurements with fewer experiments(thus increasing the information content in each experiment). Asurprisingly high level of noise in the relationship between thefluorescent signal and the protein function was measured.

Denna avhandling presenterar två projekt för att förbättrametoder för experiment med stora informationsmängderbaserade på konfokalmikroskopi.

Författarens del i det första projektet, "Toward a ConfocalSubcellular Atlas of the Human Proteome" (Mot en konfokal,subcellulär atlas av det mänskliga proteomet) var att automatiserabildinsamlingen av fyra samtidigt inmärkta strukturer i celler iplattor med 96 brunnar. Sammanlagt togs tusentals bilder avhundratals proteiner i celler. Författaren var även del i att fastställavilka bildinsamlingsmetoder som skulle användas för att maximeramängd och kvalitet på bild-informationen givet den begränsade tidper brunn som var tillgänglig för att kunna avbilda många brunnar.

Den andra studien, "Improved water permeability measurementsbased on fluorescence normalization" (Förbättrade vattenpermeabilitetsmätningargenom normalisering av fluorescens) syftade till att ökakänsligheten hos mätningar av proteiners funktion genom attnormalisera mätningarna med signalen från fluorescensen från ettkotransfekterat protein. Det skulle kunna leda till att nå tillräckligtillförlitlighet i mätresultaten med färre experiment (därmed ökainformationsinnehållet i varje experiment). En förvånansvärt högbrusnivå i förhållandet mellan fluorescenssignalen ochproteinfunktionen uppmättes

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Apeldoorn, Aart Alexander van. "Confocal Raman microscopy applications in tissue engineering /". Enschede : University of Twente [Host], 2005. http://doc.utwente.nl/50895.

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Più fonti

Libri sul tema "Confocal microscopy"

Paddock, Stephen W. Confocal Microscopy. New Jersey: Humana Press, 1998. http://dx.doi.org/10.1385/159259722x.

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Brzostowski, Joseph, e Haewon Sohn, a cura di. Confocal Microscopy. New York, NY: Springer US, 2021. http://dx.doi.org/10.1007/978-1-0716-1402-0.

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Paddock, Stephen W., a cura di. Confocal Microscopy. New York, NY: Springer New York, 2014. http://dx.doi.org/10.1007/978-1-60761-847-8.

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Tony, Wilson, a cura di. Confocal microscopy. London: Academic Press, 1990.

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Michael, Conn P., a cura di. Confocal microscopy. San Diego: Academic Press, 1999.

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Brian, Matsumoto, e American Society for Cell Biology., a cura di. Cell biological applications of confocal microscopy. 2^a ed. Amsterdam: Academic Press, 2002.

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Price, Robert L., e W. Gray (Jay) Jerome, a cura di. Basic Confocal Microscopy. New York, NY: Springer New York, 2011. http://dx.doi.org/10.1007/978-0-387-78175-4.

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Jerome, W. Gray, e Robert L. Price, a cura di. Basic Confocal Microscopy. Cham: Springer International Publishing, 2018. http://dx.doi.org/10.1007/978-3-319-97454-5.

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Toporski, Jan, Thomas Dieing e Olaf Hollricher, a cura di. Confocal Raman Microscopy. Cham: Springer International Publishing, 2018. http://dx.doi.org/10.1007/978-3-319-75380-5.

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Dieing, Thomas, Olaf Hollricher e Jan Toporski, a cura di. Confocal Raman Microscopy. Berlin, Heidelberg: Springer Berlin Heidelberg, 2011. http://dx.doi.org/10.1007/978-3-642-12522-5.

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Più fonti

Capitoli di libri sul tema "Confocal microscopy"

Bühren, Jens. "Confocal Microscopy". In Encyclopedia of Ophthalmology, 1–3. Berlin, Heidelberg: Springer Berlin Heidelberg, 2016. http://dx.doi.org/10.1007/978-3-642-35951-4_429-4.

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Cox, Guy. "Confocal Microscopy". In Springer Protocols Handbooks, 1009–25. Totowa, NJ: Humana Press, 2008. http://dx.doi.org/10.1007/978-1-60327-375-6_55.

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Sheppard, Colin J. R., e Shakil Rehman. "Confocal Microscopy". In Biomedical Optical Imaging Technologies, 213–31. Berlin, Heidelberg: Springer Berlin Heidelberg, 2012. http://dx.doi.org/10.1007/978-3-642-28391-8_6.

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Borlinghaus, Rolf Theodor. "Confocal Microscopy". In The White Confocal, 47–66. Cham: Springer International Publishing, 2017. http://dx.doi.org/10.1007/978-3-319-55562-1_3.

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Wiora, Georg, Mark Weber e Sirichanok Chanbai. "Confocal Microscopy". In Encyclopedia of Tribology, 426–34. Boston, MA: Springer US, 2013. http://dx.doi.org/10.1007/978-0-387-92897-5_314.

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Naredi-Rainer, Nikolaus, Jens Prescher, Achim Hartschuh e Don C. Lamb. "Confocal Microscopy". In Fluorescence Microscopy, 165–202. Weinheim, Germany: Wiley-VCH Verlag GmbH & Co. KGaA, 2017. http://dx.doi.org/10.1002/9783527687732.ch5.

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Naredi-Rainer, Nikolaus, Jens Prescher, Achim Hartschuh e Don C. Lamb. "Confocal Microscopy". In Fluorescence Microscopy, 175–213. Weinheim, Germany: Wiley-VCH Verlag GmbH & Co. KGaA, 2013. http://dx.doi.org/10.1002/9783527671595.ch5.

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Bühren, Jens. "Confocal Microscopy". In Encyclopedia of Ophthalmology, 475–76. Berlin, Heidelberg: Springer Berlin Heidelberg, 2018. http://dx.doi.org/10.1007/978-3-540-69000-9_429.

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Sanderson, Jeremy. "Confocal Microscopy". In Principles of Light Microscopy: From Basic to Advanced, 105–38. Cham: Springer International Publishing, 2022. http://dx.doi.org/10.1007/978-3-031-04477-9_5.

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Debarbieux, Sébastien, Amélie Boespflug, Bruno Labeille e Luc Thomas. "Confocal Microscopy". In Baran & Dawber's Diseases of the Nails and their Management, 204–11. Chichester, UK: John Wiley & Sons, Ltd, 2018. http://dx.doi.org/10.1002/9781119323396.ch8.

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Atti di convegni sul tema "Confocal microscopy"

Dixon, A. E. "Confocal microscopy". In OSA Annual Meeting. Washington, D.C.: Optica Publishing Group, 1993. http://dx.doi.org/10.1364/oam.1993.tue.1.

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Masters, Barry R., e Andreas A. Thaer. "Confocal Microscopy of the Human In Vivo Cornea". In Ophthalmic and Visual Optics. Washington, D.C.: Optica Publishing Group, 1993. http://dx.doi.org/10.1364/ovo.1993.osab.2.

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The in-vivo observation of the living cornea by the technique of confocal microscopy provides en face images of high contrast and resolution1-4 . In contrast to Nipkow disk pinhole confocal microscopes,1-4 slit based confocal systems collect more light form the eye.5-6 The development of the wide-field specular microscope by Koester was limited by the low numerical aperture of the applanating cone objective7,8. Recent developments of a high numerical aperture for the wide-field specular microscope has resulted in a confocal microscope for the eye.9,10 We describe a new flying slit confocal microscope, illuminated with a halogen lamp, which has unique imaging characteristics for in vivo human confocal microscopy.

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Webb, Robert H. "Confocal microscopy". In OSA Annual Meeting. Washington, D.C.: Optica Publishing Group, 1990. http://dx.doi.org/10.1364/oam.1990.ms1.

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Confocal microscopy is an exciting new development in imaging. The same techniques that work for compact-disk recorders make it possible to increase resolution in microscopes and to discriminate so well against scattered light that out-of-focus planes do not spoil the contrast of the focal plane. That, in turn, permits optical sectioning in the transparent objects of biology and optical profiling in metallurgy.

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Lindek, Steffen, e Ernst H. Stelzer. "Confocal theta microscopy and 4Pi-confocal theta microscopy". In IS&T/SPIE 1994 International Symposium on Electronic Imaging: Science and Technology, a cura di Carol J. Cogswell e Kjell Carlsson. SPIE, 1994. http://dx.doi.org/10.1117/12.172093.

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Zhao, Bingying, Minoru Koyama e Jerome Mertz. "High-resolution multi-z confocal microscopy". In Imaging Systems and Applications. Washington, D.C.: Optica Publishing Group, 2023. http://dx.doi.org/10.1364/isa.2023.itu3e.4.

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Multi-z confocal microscopy provides simultaneously optically-sectioned multi-plane imaging but has limited resolution. Here, we describe a novel multi-z microscope by introducing a diffractive optical element that recovers the full resolution of a conventional confocal microscope.

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Ye, P., J. L. Paredes, G. R. Arce, Y. Wu, C. Chen e D. W. Prather. "Compressive confocal microscopy". In ICASSP 2009 - 2009 IEEE International Conference on Acoustics, Speech and Signal Processing. IEEE, 2009. http://dx.doi.org/10.1109/icassp.2009.4959612.

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Chmelik, Radim. "Holographic confocal microscopy". In 12th Czech-Slovak-Polish Optical Conference on Wave and Quantum Aspects of Contemporary Optics, a cura di Jan Perina, Sr., Miroslav Hrabovsky e Jaromir Krepelka. SPIE, 2001. http://dx.doi.org/10.1117/12.417815.

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Hanna, Philip M., Brian D. Rigling e Edmund G. Zelnio. "Virtual confocal microscopy". In Electronic Imaging 2006, a cura di Brian D. Corner, Peng Li e Matthew Tocheri. SPIE, 2006. http://dx.doi.org/10.1117/12.650778.

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Sheppard, Colin J., Douglas K. Hamilton e Hubert J. Matthews. "Confocal Interference Microscopy". In 1988 International Congress on Optical Science and Engineering. SPIE, 1989. http://dx.doi.org/10.1117/12.950317.

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Sheppard, Colin J. R., e Hao Zhou. "Confocal interference microscopy". In BiOS '97, Part of Photonics West, a cura di Carol J. Cogswell, Jose-Angel Conchello e Tony Wilson. SPIE, 1997. http://dx.doi.org/10.1117/12.271254.

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Rapporti di organizzazioni sul tema "Confocal microscopy"

Hoffmeyer, Michaela. In Vivo Fluorescence Confocal Microscopy to Investigate the Role of RhoC in Inflammatory Breast Cancer. Fort Belvoir, VA: Defense Technical Information Center, aprile 2005. http://dx.doi.org/10.21236/ada435616.

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Darrow, C., T. Huser, C. Campos, M. Yan, S. Lane e R. Balhorn. Single Fluorescent Molecule Confocal Microscopy: A New Tool for Molecular Biology Research and Biosensor Development. Office of Scientific and Technical Information (OSTI), marzo 2000. http://dx.doi.org/10.2172/792442.

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Wickramaratne, Chathuri, Emily Sappington e Hanadi Rifai. Confocal Laser Fluorescence Microscopy to Measure Oil Concentration in Produced Water: Analyzing Accuracy as a Function of Optical Settings. Journal of Young Investigators, giugno 2018. http://dx.doi.org/10.22186/jyi.34.6.39-47.

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YANG, JUNYA, e NAN ZHANG ZHANG. Changes of the corneal nerve in painful diabetic neuropathy compared to painless diabetic neuropathy under corneal confocal microscopy: a systematic review and meta-analysis. INPLASY - International Platform of Registered Systematic Review and Meta-analysis Protocols, marzo 2023. http://dx.doi.org/10.37766/inplasy2023.3.0023.

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Tan, Li, Qiong Liu, Yun Chen, Ya-Qiong Zhao, Jie Zhao, Marie Aimee Dusenge, Yao Feng et al. Efficacy of sonic activation techniques on tubular dentin sealer penetration：A systematic review and meta-analysis. INPLASY - International Platform of Registered Systematic Review and Meta-analysis Protocols, luglio 2022. http://dx.doi.org/10.37766/inplasy2022.7.0116.

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Review question / Objective: Is sonic activation techniques more effective than conventional needle irrigation for the tubular dentin sealer penetration. The included study was a randomized controlled trial. Eligibility criteria: A comprehensive search was conducted for all published studies evaluating efficacy of percentage and maximum depth of sealer penetration, following the use of SI and standardized irrigants (NaOCl and EDTA). Because this can hardly be measured clinically, only confocal laser scanning microscopy (CLSM) studies were selected owing to wide use of this methodology for evaluating tubular dentin sealer penetration. The studies using previously filled roots or animal teeth, artificial debris, and plastic blocks, and studies measuring the penetration of tubular dentin sealers in lateral root canals, isthmus, or artificial grooves were excluded to maintain the standardized sample selecting and measuring (Virdee et al. 2018). The search was limited to articles published between January 2000 and June 2022 to ensure conclusions were drawn from contemporary data. There are no language restrictions on filtering articles to ensure the integrity of included data.

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George, J. S., D. M. Rector, D. M. Ranken, B. Peterson e J. Kesteron. Virtual pinhole confocal microscope. Office of Scientific and Technical Information (OSTI), giugno 1999. http://dx.doi.org/10.2172/353183.

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Or, Dani, Shmulik Friedman e Jeanette Norton. Physical processes affecting microbial habitats and activity in unsaturated agricultural soils. United States Department of Agriculture, ottobre 2002. http://dx.doi.org/10.32747/2002.7587239.bard.

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experimental methods for quantifying effects of water content and other dynamic environmental factors on bacterial growth in partially-saturated soils. Towards this end we reviewed critically the relevant scientific literature and performed theoretical and experimental studies of bacterial growth and activity in modeled, idealized and real unsaturated soils. The natural wetting-drying cycles common to agricultural soils affect water content and liquid organization resulting in fragmentation of aquatic habitats and limit hydraulic connections. Consequently, substrate diffusion pathways to soil microbial communities become limiting and reduce nutrient fluxes, microbial growth, and mobility. Key elements that govern the extent and manifestation of such ubiquitous interactions include characteristics of diffusion pathways and pore space, the timing, duration, and extent of environmental perturbations, the nature of microbiological adjustments (short-term and longterm), and spatial distribution and properties of EPS clusters (microcolonies). Of these key elements we have chosen to focus on a manageable subset namely on modeling microbial growth and coexistence on simple rough surfaces, and experiments on bacterial growth in variably saturated sand samples and columns. Our extensive review paper providing a definitive “snap-shot” of present scientific understanding of microbial behavior in unsaturated soils revealed a lack of modeling tools that are essential for enhanced predictability of microbial processes in soils. We therefore embarked on two pronged approach of development of simple microbial growth models based on diffusion-reaction principles to incorporate key controls for microbial activity in soils such as diffusion coefficients and temporal variations in soil water content (and related substrate diffusion rates), and development of new methodologies in support of experiments on microbial growth in simple and observable porous media under controlled water status conditions. Experimental efforts led to a series of microbial growth experiments in granular media under variable saturation and ambient conditions, and introduction of atomic force microscopy (AFM) and confocal scanning laser microscopy (CSLM) to study cell size, morphology and multi-cell arrangement at a high resolution from growth experiments in various porous media. The modeling efforts elucidated important links between unsaturated conditions and microbial coexistence which is believed to support the unparallel diversity found in soils. We examined the role of spatial and temporal variation in hydration conditions (such as exist in agricultural soils) on local growth rates and on interactions between two competing microbial species. Interestingly, the complexity of soil spaces and aquatic niches are necessary for supporting a rich microbial diversity and the wide array of microbial functions in unsaturated soils. This project supported collaboration between soil physicists and soil microbiologist that is absolutely essential for making progress in both disciplines. It provided a few basic tools (models, parameterization) for guiding future experiments and for gathering key information necessary for prediction of biological processes in agricultural soils. The project sparked a series of ongoing studies (at DTU and EPFL and in the ARO) into effects of soil hydration dynamics on microbial survival strategy under short term and prolonged desiccation (important for general scientific and agricultural applications).

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Martinez-Rodriguez, M. J. Laser confocal microscope for analysis of 3013 inner container closure weld region. Office of Scientific and Technical Information (OSTI), ottobre 2017. http://dx.doi.org/10.2172/1406122.

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Galbraith, David. Final Report: Confocal Laser Scanning Microscope, April 15, 1995 - April 14, 1997. Office of Scientific and Technical Information (OSTI), aprile 2000. http://dx.doi.org/10.2172/765740.

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Wendelberger, James. Template size and proper overlap detection in Laser Confocal Microscope (LCM) images. Office of Scientific and Technical Information (OSTI), agosto 2021. http://dx.doi.org/10.2172/1812643.

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