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Tesi sul tema "Cloning"

Autore: Grafiati

Pubblicato: 4 giugno 2021

Ultima modifica: 29 luglio 2024

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1

Lishchyna, V., Нiна Володимирiвна Мальована, Нина Владимировна Малеванная e Nina Volodymyrivna Malovana. "Therapeutic cloning". Thesis, Sumy State University, 2020. https://essuir.sumdu.edu.ua/handle/123456789/78011.

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The review is devoted to the current biomedical direction in cell-replacement therapy - therapeutic cloning, which is the most universal approach for obtaining patient-specific embryonic stem cell lines (hESC) with tremendous potential in maintaining and restoring human health. Now the main sources of stem cells directly for biomedical work are stem cells from umbilical cord blood and adult stem cells. Both sources have serious limitations: umbilical cord blood stem cells are autogenous only to the newly born, and receiving stem cells from the patient himself is unsafe for him. In addition, in general opinion, the potential for differentiation in these cells is lower than in ESCs. Obviously, the most versatile and reliable source of human stem cell (SC) production is through cloning technology.

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2

Koehler, Daniela. "Cloning in Cattle". Diss., lmu, 2009. http://nbn-resolving.de/urn:nbn:de:bvb:19-99159.

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3

Riley, D. J. S. "More than semantic differences obstructing a cloning ban at the United Nations /". Theological Research Exchange Network (TREN) Access this title online, 2004. http://www.tren.com.

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4

Källstrand, Björn. "Optimal, universal quantum cloning". Thesis, Karlstads universitet, Institutionen för ingenjörsvetenskap och fysik, 2013. http://urn.kb.se/resolve?urn=urn:nbn:se:kau:diva-28341.

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The no-cloning theorem is one of the fundamental concepts of quantum information theory. It tells us that no general quantum state can be perfectly replicated. In this thesis we introduce the notion of imperfect cloning, and define the properties of the universal cloning machine. Furthermore, we construct an ansatz of how our universal cloning machine should perform as to produce two imperfect clones from one input qubit. We find an optimal fidelity of 5/6 for our universal cloning machine. We then reevaluate our ansatz and construct a class of unitary transformations such that an optimal fidelity is always achieved. Lastly, we present an overview of some applications of imperfect quantum cloning in the field of quantum information.

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5

Rajagopal, Manoj Kumar. "Cloning with gesture expressivity". Phd thesis, Institut National des Télécommunications, 2012. http://tel.archives-ouvertes.fr/tel-00719301.

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Virtual environments allow human beings to be represented by virtual humans or avatars. Users can share a sense of virtual presence is the avatar looks like the real human it represents. This classically involves turning the avatar into a clone with the real human's appearance and voice. However, the possibility of cloning the gesture expressivity of a real person has received little attention so far. Gesture expressivity combines the style and mood of a person. Expressivity parameters have been defined in earlier works for animating embodied conversational agents.In this work, we focus on expressivity in wrist motion. First, we propose algorithms to estimate three expressivity parameters from captured wrist 3D trajectories: repetition, spatial extent and temporal extent. Then, we conducted perceptual study through a user survey the relevance of expressivity for recognizing individual human. We have animated a virtual agent using the expressivity estimated from individual humans, and users have been asked whether they can recognize the individual human behind each animation. We found that, in case gestures are repeated in the animation, this is perceived by users as a discriminative feature to recognize humans, while the absence of repetition would be matched with any human, regardless whether they repeat gesture or not. More importantly, we found that 75 % or more of users could recognize the real human (out of two proposed) from an animated virtual avatar based only on the spatial and temporal extents. Consequently, gesture expressivity is a relevant clue for cloning. It can be used as another element in the development of a virtual clone that represents a person

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6

Fisher, Adam B. "ex vivo DNA cloning". VCU Scholars Compass, 2015. http://scholarscompass.vcu.edu/etd/3962.

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Genetic engineering of microbes has developed rapidly along with our ability to synthesize DNA de novo. Yet, even with decreasing DNA synthesis costs there remains a need for inexpensive, rapid and reliable methods for assembling synthetic DNA into larger constructs or combinatorial libraries. While technological advances have resulted in powerful techniques for in vitro and in vivo assembly of DNA, each suffers inherent disadvantages. Here, an ex vivo DNA cloning suite using crude cellular lysates derived from E. coli is demonstrated to amplify and assemble DNA containing small sequence homologies. Further, the advantages of an ex vivo approach are leveraged to rapidly optimize several parameters of the ex vivo DNA assembly methodology testing lysates from different engineered strains of E. coli, with various buffer components and using titrations of purified cloning enzymes. Finally, in order to complete the cloning suite, a vector expressing the Pyrococcus furiosis (Pfu) DNA polymerase was designed, constructed and expressed in E. coli to create a ‘functionalized lysate’ capable of ex vivo PCR. Not only do we demonstrate ex vivo cloning methodology as a complete cloning package capable of replacing the expensive cloning reagents currently required by synthetic biologists, but also establish ex vivo as an overarching approach for conducting molecular biology.

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7

McCarrey, Sariah Cottrell. "Personhood and Cloning: Modern Applications and Ethics of Stem Cell and Cloning Technology". BYU ScholarsArchive, 2013. https://scholarsarchive.byu.edu/etd/4170.

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Within many communities and religions, including the LDS community, there is some controversy surrounding the use of stem cells – particularly embryonic stem cells (ESC). Much of this controversy arises from confusion and misconceptions about what stem cells actually are, where they come from , and when life begins. The theology of the Church of Jesus Christ of Latter-day Saints has interesting implications for the last of these considerations, and it becomes less a question of “when does life begin” and more an exploration of “when does personhood begin” or “when does the spirit enter the body.” With no official Church stance, statements from Church leaders vary on this topic, and this first section of the thesis explores the philosophical and practical meaning of personhood with a biological background intended for those not familiar with the origin or uses of stem cells.The second portion of the thesis explores possible cloning technologies. Recent events and advances address the possibility of cloning endangered and extinct species. The ethics of these types of cloning have considerations uniquely different from the type of cloning commonly practiced. Cloning of cheetahs (and other endangered or vulnerable species) may be ethically appropriate, given certain constraints. However, the ethics of cloning extinct species varies; for example, cloning mammoths and Neanderthals is more ethically problematic than conservation cloning, and requires more attention. Cloning Neanderthals in particular is likely unethical and such a project should not be undertaken. It is important to discuss and plan for the constraints necessary to mitigate the harms of conservation and extinct cloning, and it is imperative that scientific and public discourse enlighten and guide actions in the sphere of cloning.

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8

Bates, Nancy Carol. "Characterization of cbg : a cloned gene encoding an extracellular [beta]-glucosidase from Cellulomonas fimi". Thesis, University of British Columbia, 1987. http://hdl.handle.net/2429/26163.

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A group of Escherichia coli clones harbouring recombinant pBR322 plasmid, containing Cellulomonas fimi DNA inserts, that reacted with antiserum to C.fimi culture supernatant, was screened for their ability to hydrolyze carboxymethyl cellulose (CMC) and 4-methylumbeliferyll-β-D-cellobioside (MUC). A clone, pEC62, hydrolyzed MUC but did not hydrolyze CMC. The recombinant enzyme encoded by pEC62 was shown to be a β-glucosidase (cellobiase). C.fimii itself was shown to encode an extracellular β-glucosidase in C.fimi. This is the first report of an extracellular β-glucosidase from a bacterium. Deletion analysis localized the cloned gene (cbg)to the tet promoter proximal region of the 7.0 kilobase insert of pEC62. Further analysis and sequence data showed a highly active derivative of pEC62 contained a translational gene fusion between lacZ of pUC13 and cbg. From this data, a location for the cbg start site was proposed.
Science, Faculty of
Microbiology and Immunology, Department of
Graduate

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9

Pelser, Adam C. "Made in the image of man the value of Christian theology for public moral discourse on human cloning /". Electronic thesis, 2007. http://dspace.zsr.wfu.edu/jspui/handle/10339/187.

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10

Lee, Rivera Irene. "Cloning and characterization of mTom40". Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp03/MQ50814.pdf.

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11

Lee, Rivera Irene. "Cloning and characterization of mTom40". Thesis, McGill University, 1998. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=21588.

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The majority of mitochondrial proteins are synthesized on cytoplasmic polysomes and subsequently imported to a specific subcompartment within the organelle. In order to translocate cytosolic proteins into mitochondria a protein import system is located in the outer and inner mitochondrial membranes. The genetic composition of this complex has been well characterized in mitochondria of S. cerevisiae and N. crassa. However, little is known about the components of the import machinery in higher eukaryotes. Until today only homologues of Tom20 and Tom17 as well as two new proteins Metaxin and hTom34 have been identified. Nevertheless, none of the proteins forming the outer membrane translocation pore have been identified so far.
We have cloned a 35 kDa protein from a mouse cDNA library with a 25% overall aminoacid identity to yTom40 and 27% identity to nTom40. mTom40 contains two possible start codons 36 amino acids apart from each other. Interestingly, both the long and the short version of mTom40 can be imported in vitro into mouse mitochondria. This data suggests that the first 36 amino acids are not important for the import process of the protein.
The identified protein was shown to be deeply embedded into the outer membrane of mitochondria, although it is partially exposed to the intermembrane space. It possesses a sequence with very high homology to a similar region on both previously described homologues of N. crassa and S. cerevisiae. It is proposed that this region may be of physiological importance. To further characterize mTom40 we generated a yeast strain with a deletion of yTom40, and tried to rescue the lethal phenotype with the mammalian homologue. Finally we describe a bacterial overexpression system for mTom40 and a purification protocol for the fusion protein overproduced.

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12

Dent, C. L. "cDNA cloning and the retina". Thesis, University of Liverpool, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.384395.

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13

Walsh, Maura Stephanie. "Cloning fungal polyketide synthase genes". Thesis, University of Bristol, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.333957.

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14

Weinstein, Earl G. 1974. "MicroRNA cloning and bioinformatic analysis". Thesis, Massachusetts Institute of Technology, 2002. http://hdl.handle.net/1721.1/8390.

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Thesis (Ph.D.)--Massachusetts Institute of Technology, Dept. of Biology, 2002.
Includes bibliographical references.
Part I. Two gene-regulatory noncoding RNAs (ncRNAs), let-7 RNA and lin-4 RNA, were previously discovered in the C. elegans genome. The let-7 gene is conserved across a wide range of genomes, suggesting that these ncRNAs represent a wider class of gene-regulatory RNAs. Both lin-4 and let-7 RNAs are generated from stem-loop precursor RNAs, and share a common biochemical signature, namely 5'-terminal phosphate and 3'-terminal hydroxyl groups. We refer to ncRNAs that share the characteristic size, biochemical signature, and precursor structures of let-7 and lin-4 as microRNAs (miRNAs). The size of this class of genes, and its prevalence in other genomes, are unknown. Therefore, we developed an experimental and bioinformatics strategy to identify novel miRNA genes. We discovered a total of 75 miRNA genes in the C. elegans genome, and orthologues for a majority of these were computationally identified in the C. briggsae, D. melanogaster or H. sapiens genomes. Northern analysis was used to confirm and analyze the expression of these miRNAs. The data set has implications for understanding miRNA gene regulation, miRNA processing, and regulation of miRNA genes. Part II. Directed molecular evolution has previously been applied to generate RNAs with novel structures and functions. This method works because nucleic acids can be selected, randomized, amplified and characterized using polymerase chain reaction (PCR)-based methods. Here we present a novel method for extending directed molecular evolution to the realm of peptide selections by linking a peptide to its encoding mRNA.
(cont.) A proof of principle selection for two different peptides indicates that this tRNA should prove useful in discovering more complex protein molecules using directed molecular evolution.
by Earl G. Weinstein.
Ph.D.

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15

Подолкова, Світлана Віталіївна, Светлана Витальевна Подолкова, Svitlana Vitaliivna Podolkova e M. V. Ruban. "Reasons for against human cloning". Thesis, Видавництво СумДУ, 2010. http://essuir.sumdu.edu.ua/handle/123456789/18316.

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16

Gustavsson, Alma. "Cloning of an aldolase mutant". Thesis, Uppsala universitet, Institutionen för kemi - BMC, 2019. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-397493.

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17

Seto, Nina Oi Ling. "The copper-zinc superoxide dismutase gene from Drosophila melanogaster : attempts to clone the gene using two mixed sequence oligonucleotide probes". Thesis, University of British Columbia, 1987. http://hdl.handle.net/2429/26534.

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Superoxide dismutase is an enzyme which scavenges superoxide radicals and is thought to be a longevity determinant, as there exists a positive correlation between superoxide dismutase concentration and maximum life span potential. The cytosolic CuZn superoxide dismutase in D. melanogaster has been purified and sequenced, but the gene has not been cloned. However, when it is available the CuZn SOD gene may be reintroduced into the Drosophila genome via the P-element transformation system so its effects on the life span potential of Drosophila may be studied. This study describes attempts to clone the CuZn SOD gene from D. melanogaster using two mixed sequence oligonucleotide probes. The SI probe corresponds to amino acids 43-48 of the protein sequence and contains 128 different oligonucleotide sequences representing all possible codon combinations predicted from the amino acid sequence. The GT3 probe is targeted to amino acids 90-95 of the protein. In this probe, deoxyguanosine was placed in positions where all four nucleotides may occur to decrease probe heterogeneity. The probes were used to screen D. melanogaster Canton-S and Oregon-R genomic lambda libraries. Three positive clones isolated from the Canton-S library had identical nucleotide sequence in the GT3 probe binding region, and sequencing of the probe binding site revealed that one member of the GT3 probe had formed a 15 bp duplex with the phage DNA. Screening of the Oregon-R library produced four clones which hybridized with both GT3 and S1 probes. When these phage DNA were hybridized to polytene chromosomes by in situ hybridization, none mapped to 68AB on the third chromosome, the location of the CuZn SOD gene. These results suggest that modification of the classical strategy used in this study is necessary, and implications on probe design are discussed.
Medicine, Faculty of
Biochemistry and Molecular Biology, Department of
Graduate

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18

Woodruff, Wendy Anne. "Cloning and characterization of the oprF gene for protein F from Pseudomonas aeruginosa". Thesis, University of British Columbia, 1988. http://hdl.handle.net/2429/29218.

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The oprF gene encoding porin protein F from Pseudomonas aeruginosa was cloned onto a cosmid vector into Escherichia coli. Protein F was expressed in large amounts in E. coli and retained its heat- and reduction-modifiable and immunological characteristics. The cloned oprF gene product was purified from E. coli and characterized with respect to pore-forming ability in black lipid bilayers. Small channels, with an average single channel conductance of approximately 0.4 nS, were observed. A similar small channel size was observed for native protein F. The oprF sequences were used as a DNA-DNA hybridization probe with chromosomal DNA from the 17 IATS (International Antigen Typing Scheme) strains of P. aeruginosa, 52 clinical isolates and the non-aeruginosa Pseudomonads. Conservation of oprF sequences was observed among all the P. aeruginosa strains and to a lesser extent among the non-aeruginosa strains of the P. fluorescens rRNA homology group. Insertion mutations in the oprF gene were created in vivo by Tn1mutagenesis of the cloned gene in E. coli and in vitro by insertion of the streptomycin-encoding Ω fragment into the cloned gene, followed by transfer of the mutated protein F gene back into P. aeruginosa and homologous recombination with the chromosome. The oprF mutants were characterized by gel electrophoresis and immunoblotting, and it was shown that the mutants had lost protein F. The P. aeruginosa oprF mutants were characterized with respect to growth rates, antibiotic permeability and cell surface hydrophobicity. The results of these studies indicated that major alterations in the cell surface had occurred and that the cells were unable to grow in a non-defined liquid medium without added electrolytes. Marginal differences were observed in MICs (minimum inhibitory concentrations) of hydrophilic antibiotics for the oprF mutants compared with their protein F-sufficient parents. The putative roles of protein F in antibiotic permeability and general outer membrane permeability are discussed. Evidence for extensive homologies between protein F, the OmpA protein of E. coli and PHIII of Neisseria gonorrhoeae are presented. A role for protein F in prophylactic anti-Pseudomonas therapy, as a target for vaccine development, is proposed.
Science, Faculty of
Microbiology and Immunology, Department of
Graduate

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19

Boyes, Barry Edward. "Molecular cloning of the human Substantia innominata : characterization of a brain large mRNA". Thesis, University of British Columbia, 1990. http://hdl.handle.net/2429/30960.

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Brain tissue samples were collected from individuals with histologically and biochemically confirmed Alzheimer's Disease (AD), as well as from a group of individuals without any signs of neurological disease (NNC). Ribonucleic acid (RNA) was extracted from these tissues, characterized by several chemical methods, and the yields were compared between AD and NNC groups. High molecular weight RNA could be effectively extracted from frozen postmortem human brain. In comparison to the NNC group, tissue RNA levels were reduced in the AD hippocampus, but not in the temporal cortex or substantia innominata (SI). No difference in the physical integrity of the RNA was apparent between AD and NNC groups. A high complexity complementary deoxyribonucleic acid (cDNA) library was prepared using RNA extracted from the NNC SI. Differential hybridization screening using a variety of cDNA probes was employed to identify mRNAs expressed differentially between AD and NNC tissue, and between SI and other human tissues. Many selected mRNAs were examined for specificity of expression in brain tissue and brain regions. The cDNA clone pSI3a-24 identified an mRNA, which, on Northern blot hybridization, was expressed in brain tissue but not in the other human tissues examined. The identified mRNA was unusually large, with a chain length estimated at 15,500 bases. Quantification of the brain tissue levels of this mRNA was carried out using a ribonuclease protection assay. Tissue levels were higher in the SI (40 pg/μg RNA) than in the temporal cortex (28.6 pg/μg), and were lowest in the cerebellum (11.2 pg/μ9). Levels of the mRNA in temporal cortex samples were increased 29% in the AD group, relative to NNC. No significant difference in the SI tissue levels was observed between AD and NNC groups. Hybridization analysis of human genomic DNA indicated that the mRNA was encoded by a single copy gene. Sequence analysis of the full 3 kilobases of cloned cDNA was completed. Computer database searches failed to identify any known nucleic acid sequence with homology to the cDNA. Examination of the cDNA sequence for potential polypeptide coding regions suggested that the corresponding mRNA has a 3' untranslated region of at least 3 kilobases.
Medicine, Faculty of
Graduate

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20

Wakarchuk, Warren William. "The molecular cloning and characterization of a Beta-glucosidase gene from an Agrobacterium". Thesis, University of British Columbia, 1987. http://hdl.handle.net/2429/27559.

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The β-glucosidase (Abg) from ATCC 21400, an Agrobacterium species, was purified to homogeneity. The protein was cleaved with cyanogen bromide and the peptides were purified by reversed phase high pressure liquid chromatography. The partial amino-acid sequences for three CNBr peptides, CNBr1, CNBr2 and CNBr3, were determined by automated Edman degradation. A sequence from CNBr2 was used to synthesize a mixture of oligonucleotides which was used as a hybridization probe to identify a recombinant DNA clone carrying the gene for β-glucosidase. A single clone was isolated which expressed an enzymatic activity that hydrolyzed several β-glucosides. The enzymatic activity produced by this clone could be adsorbed by rabbit antiserum raised against the Agrobacterium enzyme. The direction of transcription of the β-glucosidase gene was determined by verifying the DNA sequence 3' to the oligonucleotide probe binding site. After subcloning the gene a high level of expression was obtained in the plasmid vector pUC18 using the lacZ gene promoter. The nucleotide sequence of the 1599 bp insert in pABG5 was determined using the chain terminator method. The start of the protein coding region was determined by aligning the amino terminal sequence of the protein with the predicted amino acid sequence of the cloned gene. The open reading frame was 1387 nucleotides and contained 458 codons. The molecular weight calculated from the deduced amino acid sequence agreed with that observed from both the native and recombinant enzymes. The predicted amino acid composition from the open reading frame matched with that determined for the native β-glucosidase. The stop codon of this coding region was followed by a potential stem loop structure which may be the transcriptional terminator. There was a region of the deduced Abg sequence which had homology to a region from two other β-glucosidase sequences. This region of homology contained a putative active site by analogy with the active site of hen egg white lysozyme.
Science, Faculty of
Microbiology and Immunology, Department of
Graduate

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21

Chang, Li-Ching. "Cloning, purification and crystallization of selenophosphate synthetase cloning, purification and crystallization of ERp44 from Mus musculus /". [S.l.] : [s.n.], 2006. http://deposit.ddb.de/cgi-bin/dokserv?idn=980297311.

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22

Österdahl, Fredrik, e Ilias Hendo. "Themes in Totara : Creating and cloning". Thesis, Karlstads universitet, Institutionen för matematik och datavetenskap, 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:kau:diva-47678.

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The purpose of this paper is to give the reader an insight into the graphical interface of a Learning Management system, the theme, how it functions and its importance. The main point is how the creation of new themes can be done, and two different approaches are examined and compared.The use of learning management systems have been growing rapidly over the past years. Modern learning, be it basic school, higher education or professional training, is almost always supported by some learning management system where both instructors and participators share learning material, assignments, discussion, among other things.The theme of a learning management system is of great importance and can have a substantial impact on the efficiency of the actual learning process. Themes might also differ in their purpose, thus depending on the target audience, creating a specific theme might be desirable.The work done in this paper examines the structure and function of themes in the web based Totara learning management system, and looks at two different approaches to creating new themes. These approaches involve creating something from scratch, and cloning an already existing theme.The conclusion drawn from the work done in this paper is that the most efficient way of creating a new theme, is through cloning an already existing theme. Only when time and experience is plentiful, and a full control over the theme structure is desirable, is creating a theme from scratch a viable option.

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23

Lush, Michael John. "Molecular cloning of neuropathy target esterase". Thesis, University of Leicester, 1998. http://hdl.handle.net/2381/29627.

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A single ingestion of certain organophosphorus esters (OPs) can cause a syndrome known as Organophosphate Induced Delayed Polyneuropathy (OPIDP), a paralysing neuropathy with degeneration of long axons, developing after a latent period of approximately one to three weeks. The primary target of these OPs has been shown to be a 155kDa neural protein designated Neuropathy Target Esterase (NTE), and the toxic effects apparently due to the covalent inhibition and subsequent secondary modification of this protein. Recently NTE has been purified to apparent homogeneity using a novel biotinylated OP and sufficient pure protein was produced for limited problem sequencing. The aim of this project was to clone NTE cDNA using peptide sequence data. Initially, these sequences were used to design degenerate oligonucleotide primers for amplifying sections of brain cDNA by polymerase chain reaction (PCR). These approaches were unsuccessful. Subsequently, a database searching with the peptide sequences identified a number of Expressed Sequence Tags (EST)s; these could be aligned to form an initial contig of 2.2kbp which encoded the 3' end of NTE cDNA. The 5' end of NTE cDNA, comprising a further 2.2kbp, was obtained by PCR-based technique. The final 4.4kbp contig encoded a 1327 residue polypeptide predicted to have a molecular mass of 146kDa and at least one transmembrane domain. A novel serine esterase domain of approximately 200 residues was present near the C-terminus. NTE is unrelated to any known serine hydrolases but homologous proteins are predicted to be present in diverse prokaryotic and eukaryotic organisms. The homologue in Drosophila is associated with the swisscheese phenotype, an age-dependent neurodegeneration of the brain. NTE was also mapped to chromosome 19p 13.3 between markers D19216 and the D19S413 (using the UniGene database) and an OMIM search reveals that this is near the locus of cerebellar ataxia (Cayman type).

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24

Isaac, Andrew Paul Computer Science &amp Engineering Faculty of Engineering UNSW. "Behavioural cloning robust goal directed control". Awarded By:University of New South Wales. Computer Science & Engineering, 2009. http://handle.unsw.edu.au/1959.4/43367.

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Behavioural cloning is a simple and effective technique for automatically and non-intrusively producing comprehensible and implementable models of human control skill. Behavioural cloning applies machine learning techniques to behavioural trace data, in a transparent manner, and has been very successful in a wide range of domains. The limitations of early behavioural cloning work are: that the clones lack goal-structure, are not robust to variation, are sensitive to the nature of the training data and often produce complicated models of the control skill. Recent behavioural cloning work has sought to address these limitations by adopting goal-structured task decompositions and combining control engineering representations with more sophisticated machine learning algorithms. These approaches have had some success but by compromising either transparency or robustness. This thesis addresses these limitations by investigating: new behavioural cloning representations, control structures, data processing techniques, machine learning algorithms, and performance estimation and testing techniques. First a novel hierarchical decomposition of control is developed, where goal settings and the control skill to achieve them are learnt. This decomposition allows feedback control mechanisms to be combined with modular goal-achievement. Data processing limitations are addressed by developing data-driven, correlative and sampling techniques, that also inform the development of the learning algorithm. The behavioural cloning process is developed by performing experiments on simulated aircraft piloting tasks, and then the generality of the process is tested by performing experiments on simulated gantry-crane control tasks. The performance of the behavioural cloning process was compared to existing techniques, and demonstrated a marked improvement over these methods. The system is capable of handling novel goal-settings and task structure, under high noise conditions. The ability to produce successful controllers was greatly improved by using the developed control representation, data processing and learning techniques. The models produced are compact but tend to abstract the originating control behaviour. In conclusion, the control representation and cloning process address current limitations of behavioural cloning, and produce reliable, reusable and readable clones.

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25

Jeromin, Andreas. "Frequenin in crustaceans, cloning and immunolocalization". Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp01/MQ33955.pdf.

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26

Lundberg, Mathias. "Cloning and characterization of human glutaredoxins /". Stockholm, 2002. http://diss.kib.ki.se/2002/91-7349-261-2.

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27

Tsoi, Carrie. "Cloning and characterization of canine sulfotransferases /". Stockholm, 2003. http://diss.kib.ki.se/2003/91-7349-679-0/.

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28

Long, Graham Stanley. "Molecular cloning of bacteriophage K1E endosialidase". Thesis, University of Cambridge, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.339539.

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29

Sakuntabhai, Anavaj. "Positional cloning of Darier's disease gene". Thesis, University of Oxford, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.302392.

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30

Dunn, Alison M. "Cloning of human DNA repair genes". Thesis, University of Oxford, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.301385.

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31

McNair, Alan Thomas. "Molecular cloning of Fasciola hepatica antigens". Thesis, Queen's University Belfast, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.335604.

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32

Reza, Faisal 1980. "Human cloning : science, ethics, policy, society". Thesis, Massachusetts Institute of Technology, 2003. http://hdl.handle.net/1721.1/29582.

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Thesis (B.S.)--Massachusetts Institute of Technology, Program in Science, Technology, and Society, 2003.
Includes bibliographical references (leaves 73-74).
The interplay of science, ethics, policy and society contribute to our understanding of and relation with human cloning. Genetic science and technology at the end of the twentieth century has permitted successful cloning of mammals and other animals. Such advancement has raised key ethical issues regarding the prospect of cloning human beings. Evaluation of these issues has led to policies aimed at regulating this novel technology. In tum, these policies strive to prepare our society for the scientific possibilities and ethical implications of human cloning.
by Faisal Reza.
B.S.

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33

Martínez-García, Juan Carlos. "Expression cloning of insecticidal toxin receptors". Thesis, University of Cambridge, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.621840.

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34

Hinson, Gary. "Cloning of an Escherichia coli adhesin". Thesis, University of Leicester, 1987. http://hdl.handle.net/2381/34424.

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Pathogenic bacteria colonise their host animals by means of a complex set of interactions. The host defensive mechanisms attack foreign microorganisms and attempt to rid the animal of the invaders, while the bacteria express a variety of functions to ensure their survival under adverse conditions, some of which damage the host and cause the clinical symptoms of disease. Adhesins are the bacterial structures which mediate adherence to specific host tissues and therefore permit the colonisation of areas from which the bacteria would normally be removed. T4. I have genetically cloned and analysed an adhesin from a pathogenic strain of Escherichia coli isolated from a child with enteritis. The genetic information was transferred to laboratory strains of E. coli and was expressed under similar conditions as in the parent strain, generating material with the same adherence and antigenic properties. Thus, the cloned genes enabled laboratory strains to adhere to human colon, but not to duodenum, in the same manner as the parent. This probably accounts in large part for the tissue specificity of the pathogen which caused dysentery-like symptoms consistent with colonisation of, and damage to, the colon. The cloned genes encoded the synthesis of the adhesin as fine fibrils ('fimbriae') on the bacterial surface, approximately 2 nm in diameter. The 14,000 dalton protein subunits were assembled into very high molecular weight aggregates and were purified by size fractionation. The genetic determinant occupied about 6,000 basepairs of DNA, indicating a system of genes for the synthesis, export and assembly of functional adhesin. The genetic map was very similar to those of adhesins from another enteritis isolate and a urinary tract pathogen, suggesting an evolutionary relationship between these E. coli strains. However, the protein subunits of the three adhesins appear to differ, indicating a degree of divergence.

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35

Yamada, Yuichiro. "Cloning of a somatostatin receptor family". Kyoto University, 1994. http://hdl.handle.net/2433/160717.

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本文データは平成22年度国立国会図書館の学位論文(博士)のデジタル化実施により作成された画像ファイルを基にpdf変換したものである
Kyoto University (京都大学)
0048
新制・課程博士
博士(医学)
甲第5815号
医博第1594号
新制||医||592(附属図書館)
UT51-94-X197
京都大学大学院医学研究科内科系専攻
(主査)教授中尾一和, 教授成宮周, 教授森徹
学位規則第4条第1項該当

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36

Brihn, Lesil E. "POSITIONAL CLONING OF THE DISORGANIZATION MUTATION". Case Western Reserve University School of Graduate Studies / OhioLINK, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=case1189146887.

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37

Choi, Wai To. "Molecular cloning of ribosome-inactivating proteins". HKBU Institutional Repository, 1996. http://repository.hkbu.edu.hk/etd_ra/63.

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38

Pratap, Amrit Abu-Mostafa Yaser S. Abu-Mostafa Yaser S. "Adaptive learning algorithms and data cloning /". Diss., Pasadena, Calif. : Caltech, 2008. http://resolver.caltech.edu/CaltechETD:etd-05292008-231048.

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39

Moser, Bernhard. "Molecular cloning, characterization and expression of the endoglucanase C gene of Cellulomonas fimi and properties of the native and recombinant gene products". Thesis, University of British Columbia, 1988. http://hdl.handle.net/2429/29036.

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In addition to substrate-associated cellulases, Cellulomonas fimi secretes endoglucanases ( endo-1, 4-β-D-glucan glucanohydrolases, EC 3.2.1.4. ) which are recovered from the cellulose-free culture supernatant of cells grown on microcrystalline cellulose. Two such enzymes, C3.1 and C3.2 with Mrs of 130'000 and 120'000, respectively, were purified to homogeneity. The two endoglucanases were shown to share the same N-terminal amino acid sequence and to hydrolyze carboxymethylcellulose ( CMC ) with similar efficiencies ( 236u/mg protein for C3.1 and 367u/mg protein for C3.2 ). The recombinant lambda vector L47.1-169 was identified from a C.fimi DNA-lambda library on the basis of hybridization with C3.1/2-specific oligonucleotide probes. The subclone pTZ18R-8 only moderately expressed CMCase activity. The 5'-terminus of cenC ( the gene coding for C3.1/2 ) was localized in the insert by Southern transfer experiments and nucleotide sequence analysis. Results from total C.fimi RNA-DNA hybrid protection analyses defined the boundaries of cenC in pTZ18R-8 and led to the tentative identification of -10 and -35 promoter sequences. To improve the expression of cenC, its entire coding sequence, except for the start codon GTG, was fused in frame to the ATG codon of a synthetic ribosomal binding site ( PTIS ) and placed under the transcriptional control of the lac p/o system. Induction of the resulting clone ( JM101[pTZP-cenC] ) led to impaired growth in liquid cultures because overproduction of CenC inhibited cell division'" and eventually led to cell death. Analysis of cell fractions by SDS-PAGE revealed a dominant ( >10% of total cell extract proteins ), clone-specific protein with a Mr of approximately 140'000 which was found exclusively in the cytoplasmic fraction. Conversely, 60% of the total CMC-hydrolyzing activity was localized in the periplasmic fraction indicating that the export of CenC is required for maximal expression of endoglucanase activity. Isolation of the cellulolytic activities from an osmotic shockate led to the purification to homogeneity of two recombinant cellulases, CenC1 and CenC2, with Mr of 130'000 and 120'000, respectively. Both enzymes hydrolyzed CMC with similar efficiencies ( 278u/mg protein for CenC1 and 390u/mg protein for CenC2 ). In addition, amino acid sequence analyses showed the two enzymes to have the same N-termini as the native enzymes and proved furthermore that the CenC leader peptide was functional in Escherichia coli.
Science, Faculty of
Microbiology and Immunology, Department of
Graduate

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40

Doucette, Stephanie A. "Cloning of Bovine Placental Lactogen and Production in Vitro". Fogler Library, University of Maine, 2003. http://www.library.umaine.edu/theses/pdf/DoucetteSA2003.pdf.

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41

Tsie, Marlene S. "Cloning and Immunolocalization of G Proteins in the Spionid Polychaete Dipolydora quadrilobata". Fogler Library, University of Maine, 2006. http://www.library.umaine.edu/theses/pdf/TsieMS2006.pdf.

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42

Zhang, Ling 1962. "Molecular cloning and characterization of the chicken ornithine decarboxylase gene". Thesis, McGill University, 1994. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=22831.

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Ornithine decarboxylase (ODC) is the rate determining enzyme in the biosynthesis of polyamines which are essential for cell growth. In chickens, significantly higher bioactivity is reported in broiler than in egg layer strains of chickens (Bulfield et al., 1988). To characterize the genetic differences in growth rates and ODC levels in chickens, an ODC cDNA and genomic gene were cloned and sequenced. Sequencing of ODC cDNA revealed that this clone (pODZ3: 2,052 bp) was not a full length of ODC cDNA and contained 2 putative introns. The open reading frame (introns deleted) coded for a protein of 404 amino acids which had about 85% amino acid identity with human ODC. Sequencing of genomic ODC clone (pODG2-8: 5098 bp) represented the 3$ prime$ end of ODC gene from downstream of intron 7. Northern blotting of chicken RNA probed with the insert of pODZ3 revealed 2 hybridizable messages of 1.6 and 2.1 kb, respectively. In addition, analysis of MspI restriction fragment length polymorphism (RFLP) using the 3$ prime$ end of ODC gene as a probe suggested that two MspI RFLPs present in the lean line of broiler chickens was related to selection of high lean body mass.

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43

Suen, Ki-Ling. "Cloning of protein kinase genes from a carrot cDNA library". Diss., Georgia Institute of Technology, 1991. http://hdl.handle.net/1853/25431.

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44

Cammas, Florence Marie. "Characterisation of the mouse ACTH receptor promoter". Thesis, Queen Mary, University of London, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.244208.

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45

Barton, Jenny Loretta. "IL-1L1 : a novel gene in the human interleukin-1 cluster". Thesis, University of Sheffield, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.366223.

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46

Wain, Hester Mary. "Targeted mapping of the chicken genome". Thesis, University of Hertfordshire, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.338594.

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47

Godwin, Rosamond Mary. "Cloning and characterisation of genes encoding phosphate and sulphate transporters from rice /". St. Lucia, Qld, 2002. http://www.library.uq.edu.au/pdfserve.php?image=thesisabs/absthe16400.pdf.

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48

Freeman, John Douglas. "The cloning of polyhomeotic, a complex Drosophila locus required for segment determination and cuticular differentiation". Thesis, University of British Columbia, 1987. http://hdl.handle.net/2429/26256.

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The polyhomeotic (ph) locus of Drosophila melanogaster has been characterized genetically. Early studies showed that ph is a member of the Polycomb (Pc) group. These genes have similar phenotypes and are required for normal segment determination. Recent analyses of amorphic ph mutations show that the ph locus is complex, has a strong maternal effect and plays a role in cuticular development. To test the function of ph at the molecular level, the cloning of the ph locus was undertaken. One strain had been shown to contain a P element insertion near ph. A genomic library was prepared from this strain and a recombinant phage containing this P element insertion was isolated by transposon tagging. The DNA flanking the insertion was used as a starting point for a chromosomal walk. A series of overlapping phage spanning 170 kilobases was isolated. Southern blot analysis was used to determine the locations of important deficiency breakpoints within the region covered by the walk. A distance of approximately 35 kb was shown to separate the two deficiency breakpoints which include ph. This interval was found to contain rearrangements in four of the seven ph alleles which were examined by Southern blot analysis. The interval also contains a repeated sequence. The relationship between the genetic and molecular structure of ph is discussed.
Science, Faculty of
Zoology, Department of
Graduate

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49

Mirhosseini, Negin. "Cloning and expression of equine NF-kB2". [College Station, Tex. : Texas A&M University, 2008. http://hdl.handle.net/1969.1/ETD-TAMU-2838.

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50

Skeggs, Patricia Ann. "Cloning of aminoglycoside-resistance determinants in Streptomyces". Thesis, University of Leicester, 1986. http://hdl.handle.net/2381/35152.

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