Bibliografie tematiche / Bifidobacterium

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Letteratura scientifica selezionata sul tema "Bifidobacterium"

Autore: Grafiati
Pubblicato: 4 giugno 2021
Ultima modifica: 14 settembre 2024

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Articoli di riviste sul tema "Bifidobacterium"

1

Turroni, Francesca, Elena Foroni, Paola Pizzetti, Vanessa Giubellini, Angela Ribbera, Paolo Merusi, Patrizio Cagnasso et al. "Exploring the Diversity of the Bifidobacterial Population in the Human Intestinal Tract". Applied and Environmental Microbiology 75, n. 6 (23 gennaio 2009): 1534–45. http://dx.doi.org/10.1128/aem.02216-08.

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ABSTRACT Although the health-promoting roles of bifidobacteria are widely accepted, the diversity of bifidobacteria among the human intestinal microbiota is still poorly understood. We performed a census of bifidobacterial populations from human intestinal mucosal and fecal samples by plating them on selective medium, coupled with molecular analysis of selected rRNA gene sequences (16S rRNA gene and internally transcribed spacer [ITS] 16S-23S spacer sequences) of isolated colonies. A total of 900 isolates were collected, of which 704 were shown to belong to bifidobacteria. Analyses showed that the culturable bifidobacterial population from intestinal and fecal samples include six main phylogenetic taxa, i.e., Bifidobacterium longum, Bifidobacterium pseudocatenulatum, Bifidobacterium adolescentis, Bifidobacterium pseudolongum, Bifidobacterium breve, and Bifidobacterium bifidum, and two species mostly detected in fecal samples, i.e., Bifidobacterium dentium and Bifidobacterium animalis subp. lactis. Analysis of bifidobacterial distribution based on age of the subject revealed that certain identified bifidobacterial species were exclusively present in the adult human gut microbiota whereas others were found to be widely distributed. We encountered significant intersubject variability and composition differences between fecal and mucosa-adherent bifidobacterial communities. In contrast, a modest diversification of bifidobacterial populations was noticed between different intestinal regions within the same individual (intrasubject variability). Notably, a small number of bifidobacterial isolates were shown to display a wide ecological distribution, thus suggesting that they possess a broad colonization capacity.
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2

Dinoto, Achmad, Tatiana M. Marques, Kanta Sakamoto, Satoru Fukiya, Jun Watanabe, Susumu Ito e Atsushi Yokota. "Population Dynamics of Bifidobacterium Species in Human Feces during Raffinose Administration Monitored by Fluorescence In Situ Hybridization-Flow Cytometry". Applied and Environmental Microbiology 72, n. 12 (20 ottobre 2006): 7739–47. http://dx.doi.org/10.1128/aem.01777-06.

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ABSTRACT The population dynamics of bifidobacteria in human feces during raffinose administration were investigated at the species level by using fluorescence in situ hybridization (FISH) coupled with flow cytometry (FCM) analysis. Although double-staining FISH-FCM using both fluorescein isothiocyanate (FITC) and indodicarbocyanine (Cy5) as labeling dyes for fecal samples has been reported, the analysis was interfered with by strong autofluorescence at the FITC fluorescence region because of the presence of autofluorescence particles/debris in the fecal samples. We circumvented this problem by using only Cy5 fluorescent dye in the FISH-FCM analysis. Thirteen subjects received 2 g of raffinose twice a day for 4 weeks. Fecal samples were collected, and the bifidobacterial populations were monitored using the established FISH-FCM method. The results showed an increase in bifidobacteria from about 12.5% of total bacteria in the prefeeding period to about 28.7 and 37.2% after the 2-week and 4-week feeding periods, respectively. Bifidobacterium adolescentis, the Bifidobacterium catenulatum group, and Bifidobacterium longum were the major species, in that order, at the prefeeding period, and these bacteria were found to increase nearly in parallel during the raffinose administration. During the feeding periods, indigenous bifidobacterial populations became more diverse, such that minor species in human adults, such as Bifidobacterium breve, Bifidobacterium bifidum, Bifidobacterium dentium, and Bifidobacterium angulatum, proliferated. Four weeks after raffinose administration was stopped, the proportion of each major bifidobacterial species, as well as that of total bifidobacteria, returned to approximately the original values for the prefeeding period, whereas that of each minor species appeared to differ considerably from its original value. To the best of our knowledge, these results provide the first clear demonstration of the population dynamics of indigenous bifidobacteria at the species level in response to raffinose administration.
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3

Requena, Teresa, Jeremy Burton, Takahiro Matsuki, Karen Munro, Mary Alice Simon, Ryuichiro Tanaka, Koichi Watanabe e Gerald W. Tannock. "Identification, Detection, and Enumeration of Human Bifidobacterium Species by PCR Targeting the Transaldolase Gene". Applied and Environmental Microbiology 68, n. 5 (maggio 2002): 2420–27. http://dx.doi.org/10.1128/aem.68.5.2420-2427.2002.

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ABSTRACT Methods that enabled the identification, detection, and enumeration of Bifidobacterium species by PCR targeting the transaldolase gene were tested. Bifidobacterial species isolated from the feces of human adults and babies were identified by PCR amplification of a 301-bp transaldolase gene sequence and comparison of the relative migrations of the DNA fragments in denaturing gradient gel electrophoresis (DGGE). Two subtypes of Bifidobacterium longum, five subtypes of Bifidobacterium adolescentis, and two subtypes of Bifidobacterium pseudocatenulatum could be differentiated using PCR-DGGE. Bifidobacterium angulatum and B. catenulatum type cultures could not be differentiated from each other. Bifidobacterial species were also detected directly in fecal samples by this combination of PCR and DGGE. The number of species detected was less than that detected by PCR using species-specific primers targeting 16S ribosomal DNA (rDNA). Real-time quantitative PCR targeting a 110-bp transaldolase gene sequence was used to enumerate bifidobacteria in fecal samples. Real-time quantitative PCR measurements of bifidobacteria in fecal samples from adults correlated well with results obtained by culture when either a 16S rDNA sequence or the transaldolase gene sequence was targeted. In the case of samples from infants, 16S rDNA-targeted PCR was superior to PCR targeting the transaldolase gene for the quantification of bifidobacterial populations.
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4

Sakurai, Takuma, Toshitaka Odamaki e Jin-zhong Xiao. "Production of Indole-3-Lactic Acid by Bifidobacterium Strains Isolated fromHuman Infants". Microorganisms 7, n. 9 (11 settembre 2019): 340. http://dx.doi.org/10.3390/microorganisms7090340.

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Recent studies have shown that metabolites produced by microbes can be considered as mediators of host-microbial interactions. In this study, we examined the production of tryptophan metabolites by Bifidobacterium strains found in the gastrointestinal tracts of humans and other animals. Indole-3-lactic acid (ILA) was the only tryptophan metabolite produced in bifidobacteria culture supernatants. No others, including indole-3-propionic acid, indole-3-acetic acid, and indole-3-aldehyde, were produced. Strains of bifidobacterial species commonly isolated from the intestines of human infants, such as Bifidobacterium longum subsp. longum, Bifidobacterium longum subsp. infantis, Bifidobacterium breve, and Bifidobacterium bifidum, produced higher levels of ILA than did strains of other species. These results imply that infant-type bifidobacteria might play a specific role in host–microbial cross-talk by producing ILA in human infants.
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5

Mikkelsen, Lene Lind, Christian Bendixen, Mogens Jakobsen e Bent Borg Jensen. "Enumeration of Bifidobacteria in Gastrointestinal Samples from Piglets". Applied and Environmental Microbiology 69, n. 1 (gennaio 2003): 654–58. http://dx.doi.org/10.1128/aem.69.1.654-658.2003.

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Abstract (sommario):
ABSTRACT The population of Bifidobacterium spp. in fecal samples from suckling piglets was investigated, and Beerens, raffinose-bifidobacterium (RB), and modified Wilkins-Chalgren (MW) agar media were evaluated with regard to the enumeration of bifidobacteria in porcine intestinal samples. The results demonstrated that the population of bifidobacteria in the feces of suckling piglets is numerically low, and a phylogenetic analysis of the 16S rRNA gene from bifidobacterial isolates suggested that a possibly new Bifidobacterium species was isolated. Beerens, RB, and MW agar media were not selective for bifidobacteria in the fecal samples. The highest recovery and diversity of bifidobacteria were obtained for MW agar. Nonbifidobacterial isolates from the three agar media were identified and may contribute to the future formulation of improved selective media for the enumeration of bifidobacteria.
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6

Matsuki, Takahiro, Koichi Watanabe, Junji Fujimoto, Yukiko Kado, Toshihiko Takada, Kazumasa Matsumoto e Ryuichiro Tanaka. "Quantitative PCR with 16S rRNA-Gene-Targeted Species-Specific Primers for Analysis of Human Intestinal Bifidobacteria". Applied and Environmental Microbiology 70, n. 1 (gennaio 2004): 167–73. http://dx.doi.org/10.1128/aem.70.1.167-173.2004.

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ABSTRACT A highly sensitive quantitative PCR detection method has been developed and applied to the distribution analysis of human intestinal bifidobacteria by combining real-time PCR with Bifidobacterium genus- and species-specific primers. Real-time PCR detection of serially diluted DNA extracted from cultured bifidobacteria was linear for cell counts ranging from 106 to 10 cells per PCR assay. It was also found that the method was applicable to the detection of Bifidobacterium in feces when it was present at concentrations of >106 cells per g of feces. Concerning the distribution of Bifidobacterium species in intestinal flora, the Bifidobacterium adolescentis group, the Bifidobacterium catenulatum group, and Bifidobacterium longum were found to be the three predominant species by examination of DNA extracted from the feces of 46 healthy adults. We also examined changes in the population and composition of Bifidobacterium species in human intestinal flora of six healthy adults over an 8-month period. The results showed that the composition of bifidobacterial flora was basically stable throughout the test period.
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7

Fushinobu, Shinya, e Maher Abou Hachem. "Structure and evolution of the bifidobacterial carbohydrate metabolism proteins and enzymes". Biochemical Society Transactions 49, n. 2 (5 marzo 2021): 563–78. http://dx.doi.org/10.1042/bst20200163.

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Abstract (sommario):
Bifidobacteria have attracted significant attention because they provide health-promoting effects in the human gut. In this review, we present a current overview of the three-dimensional structures of bifidobacterial proteins involved in carbohydrate uptake, degradation, and metabolism. As predominant early colonizers of the infant's gut, distinct bifidobacterial species are equipped with a panel of transporters and enzymes specific for human milk oligosaccharides (HMOs). Interestingly, Bifidobacterium bifidum and Bifidobacterium longum possess lacto-N-biosidases with unrelated structural folds to release the disaccharide lacto-N-biose from HMOs, suggesting the convergent evolution of this activity from different ancestral proteins. The crystal structures of enzymes that confer the degradation of glycans from the mucin glycoprotein layer provide a structural basis for the utilization of this sustainable nutrient in the gastrointestinal tract. The utilization of several plant dietary oligosaccharides has been studied in detail, and the prime importance of oligosaccharide-specific ATP-binding cassette (ABC) transporters in glycan utilisations by bifidobacteria has been revealed. The structural elements underpinning the high selectivity and roles of ABC transporter binding proteins in establishing competitive growth on preferred oligosaccharides are discussed. Distinct ABC transporters are conserved across several bifidobacterial species, e.g. those targeting arabinoxylooligosaccharide and α-1,6-galactosides/glucosides. Less prevalent transporters, e.g. targeting β-mannooligosaccharides, may contribute to the metabolic specialisation within Bifidobacterium. Some bifidobacterial species have established symbiotic relationships with humans. Structural studies of carbohydrate-utilizing systems in Bifidobacterium have revealed the interesting history of molecular coevolution with the host, as highlighted by the early selection of bifidobacteria by mucin and breast milk glycans.
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8

Saturio, Silvia, Alicja M. Nogacka, Guadalupe M. Alvarado-Jasso, Nuria Salazar, Clara G. de los Reyes-Gavilán, Miguel Gueimonde e Silvia Arboleya. "Role of Bifidobacteria on Infant Health". Microorganisms 9, n. 12 (23 novembre 2021): 2415. http://dx.doi.org/10.3390/microorganisms9122415.

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Abstract (sommario):
Bifidobacteria are among the predominant microorganisms during infancy, being a dominant microbial group in the healthy breastfed infant and playing a crucial role in newborns and infant development. Not only the levels of the Bifidobacterium genus but also the profile and quantity of the different bifidobacterial species have been demonstrated to be of relevance to infant health. Although no definitive proof is available on the causal association, reduced levels of bifidobacteria are perhaps the most frequently observed alteration of the intestinal microbiota in infant diseases. Moreover, Bifidobacterium strains have been extensively studied by their probiotic attributes. This review compiles the available information about bifidobacterial composition and function since the beginning of life, describing different perinatal factors affecting them, and their implications on different health alterations in infancy. In addition, this review gathers exhaustive information about pre-clinical and clinical studies with Bifidobacterium strains as probiotics in neonates.
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9

Peirotén, A., J. L. Arqués, M. Medina e E. Rodríguez-Mínguez. "Bifidobacterial strains shared by mother and child as source of probiotics". Beneficial Microbes 9, n. 2 (27 febbraio 2018): 231–38. http://dx.doi.org/10.3920/bm2017.0133.

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Abstract (sommario):
Importance of bifidobacteria as part of the infant intestinal microbiota has been highlighted. Their acquisition is influenced by the mode of birth and the feed regime afterwards, with a special role of the maternal microbiota. The presence of the same shared bifidobacterial strains between breast milk and infant faeces in 14 mother-infant pairs was assessed by means of pulsed-field gel electrophoresis (PFGE) genotyping. Four shared strains of Bifidobacterium breve (2), Bifidobacterium longum subsp. infantis and B. longum subsp. longum were found in breast milk-infant faeces pairs. Two years later, a second survey yielded four shared strains of the species Bifidobacterium adolescentis, Bifidobacterium bifidum, B. longum subsp. longum and Bifidobacterium pseudocatenulatum. Moreover, a B. bifidum strain was found to be shared by the infant faeces of the first study and the mother faeces tested two years later, pointing out a long term persistence. Some of the selected bifidobacterial strains showed probiotic potential due to their survival to gastrointestinal conditions and their ability to form biofilms.
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10

ROY, DENIS, e PIERRE WARD. "Rapid Detection of Bifidobacterium dentium by Enzymatic Hydrolysis of β-Glucuronide Substrates". Journal of Food Protection 55, n. 4 (1 aprile 1992): 291–95. http://dx.doi.org/10.4315/0362-028x-55.4.291.

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Abstract (sommario):
Enzyme profiles and fermentation patterns of bifidobacteria were studied to determine phenotypic characteristics that allow the rapid detection of Bifidobacterium dentium and its differentiation from Bifidobacterium adolescentis, Bifidobacterium angulatum, Bifidobacterium catenulatum, and Bifidobacterium pseudocatenulatum. Among 43 bifidobacterial strains tested, the production of β-glucuronidase was limited to six strains of B. dentium. The presence of B. dentium on a selective medium may be rapidly confirmed by the detection of β-glucuronidase activity. Columbia agar containing propionic acid was chosen to enumerate bifidobacteria previously cultivated in MRS medium. After 48 h of incubation, β-glucuronidase activity was determined by using a plate staining procedure. B. dentium strains gave positive results for β-glucuronidase activity after application of the overlay solution of β-glucuronide substrate. The β-glucuronidase assay is a rapid screening method for B. dentium. This method might be useful for selection of nonpathogenic strains or detection of fecal contamination from human origin.
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Più fonti

Tesi sul tema "Bifidobacterium"

1

Васильєва, К. О., e І. М. Волошина. "Біотехнологічні аспекти Bifidobacterium". Thesis, Київський національний університет технологій та дизайну, 2020. https://er.knutd.edu.ua/handle/123456789/15599.

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2

Bahaka, Driss. "Analyse phenotypique et genotypique des souches du genre bifidobacterium appartenant ou apparentees aux especes b. Breve, b. Infantis et b. Longum". Lille 2, 1993. http://www.theses.fr/1993LIL2P254.

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3

Grill, Jean-Pierre. "Étude du potentiel probiotique de bactéries du genre Bifidobacterium : purification et caractérisation de la fructose 6 phosphate phosphocetolase de Bifidobacterium longum et Bifidobacterium animalis". Nancy 1, 1995. http://www.theses.fr/1995NAN10050.

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Abstract (sommario):
Cette étude a permis de montrer les effets de certaines souches de bactéries appartenant au genre Bifidobacterium sur la flore intestinale, les nitrites, les nitrosamines et les sels biliaires. L’enzyme impliquée dans la déconjugaison des sels biliaires a été purifiée et caractérisée pour la souche de Bifidobacterium longum BB536. La fructose 6 phosphate phosphocétolase a été purifiée et caractérisée chez différentes souches de bifidobactéries. Des séquences en acides aminés de cette protéine ont ainsi été obtenues pour Bifidobacterium longum et Bifidobacterium animalis
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4

Oberg, Taylor S. "Characterization of the Hydrogen Peroxide Stress Responses of Bifidobacterium longum and Bifidobacterium animalis subsp. Lactis". DigitalCommons@USU, 2013. https://digitalcommons.usu.edu/etd/2037.

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Abstract (sommario):
Probiotics are living organisms which exert a beneficial health effect when consumed in sufficient numbers. Consumer interest in probiotics has increased dramatically in recent years prompting an increase in production and development of functional foods. One major problem is the decreased viability of probiotic bacteria during functional food production and storage and subsequent digestion due to environmental stresses. The most common probiotic strains belong to the genus Lactobacillus or Bifidobacterium. Due to the anaerobic nature of these bacteria, they lack the required defense mechanisms for oxidative stress inherent in aerobic microorganisms. This study examined the oxidative stress responses of six strains of Bifidobacterium, which are commonly used as probiotics in functional foods.The first phase of the study investigated the innate and inducible hydrogen peroxide (H2O2) stress response of Bifidobacterium longum strains NCC2705 and D2957, Bifidobacterium longum ssp. infantis ATCC 15697, and Bifidobacterium animalis ssp. lactis strains BL-04, DSM10140 and RH-1. Strains were screened for survival at increasing concentrations of H2O2 and lethal and sublethal concentrations were determined for each. In the second phase, B. animalis ssp. lactis strains BL-04 and DSM10140 and B. longum strains NCC2705 and D2957 were treated with a sublethal H2O2 concentration and RNA samples were collected for transcriptome analysis after 5 min and either 20 or 60 min. Statistical analysis was performed to identify genes that increased or decreased in expression during H2O2 treatment compared to control cells.Results showed that survival was species and strain dependent and that strains which naturally survived higher H2O2 concentrations had a larger number of differentially expressed genes early on during H2O2 exposure. Some of the protective genetic systems that were activated during H2O2 stress are mechanisms which perform basic cellular functions under normal conditions such as deoxuynucleotide synthesis. Under stress conditions, these systems can be used to detoxify oxidative free radicals. Also a number of genes involved in sugar transport and energy production for the cell showed increased expression, which reveals the increased energy needs of the cells during oxidative stress.During testing, it was found that two B. animalis ssp. lactis strains, BL-04 and DSM10140, had differing levels of survival and gene expression during H2O2 exposure despite having almost identical genome sequences. It was determined that one possible cause of the differences was a genetic deletion in a gene that allows the cell to incorporate extracellular fatty acids into the cell membrane instead of synthesizing them.Results from this project have increased the understanding of oxidative stress responses in bifidobacteria and highlighted possible methods to increase bacterial survival during food manufacture, storage, and human digestion.
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5

Nunoura, Naoki. "Studies on Bifidobacterium breve β-Glucosidase". Kyoto University, 1997. http://hdl.handle.net/2433/202382.

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Kyoto University (京都大学)
0048
新制・課程博士
博士(農学)
甲第6899号
農博第917号
新制||農||739(附属図書館)
学位論文||H9||N3023(農学部図書室)
16016
UT51-97-H283
京都大学大学院農学研究科食品工学専攻
(主査)教授 熊谷 英彦, 教授 木村 光, 教授 清水 昌
学位規則第4条第1項該当
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6

Hassi, Chafiq. "Contribution a l'etude d'utilisation de la n-acetyl-beta-d-glucosamine par b. Bifidum atcc 15696 et b. Animalis atcc 25527 : preparation, purification et caracterisation de leur n-acetyl-beta-d-glucosaminidase". Lille 2, 1994. http://www.theses.fr/1994LIL2P253.

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7

Mizerovská, Lucie. "Selektivní izolace bakterií rodu Bifidobacterium z potravin". Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2012. http://www.nusl.cz/ntk/nusl-216880.

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Probiotic lactic acid bacteria (LAB) are very often used in food procesing industry, such as milk products, cheese and fermentsd salami production in nova days. In diploma thesis were tested symbiotic food supplements from different producers. Bacterial DNA was isolated from crude cell lysates of six food suplements by magnetic particles P(HEMA-co-GMA). PCR-ready DNAs were isolated. from all products The detection of Bifidobacterium bacteria identified by PCR was in agreement with those declared by the manufacturers. Magnetic particles with immobilized antibodies against Bifidobacterium were used in the next part of thesis. These particles were used for the isolation of target cells from two products with cell identification by genus specific PCR.
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8

Orrhage, Kerstin. "Impact of Bifidobacterium longum and Lactobacillus acidophilus on the intestinal microflora and bioavailability of some food mutagens /". Stockholm, 1999. http://diss.kib.ki.se/1999/91-628-3687-0/.

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9

Appourchaux, Laurence. "Purification et propriétés des béta-D-galactosidases des N-acétyl-béta-D-glucosaminidases de Bifidobacterieum bifidum souche AA 2/2". Lille 1, 1989. http://www.theses.fr/1989LIL10164.

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Quatre activités exoglycosidasiques de bifidobacterium bifidum souche AA/22 ont été étudiées. Il s'agit des activités: béta-galactosidase, n-acétyl-beta-hexosaminidase, alpha-neuraminidase et alpha-fucosidase. Ces activités sont endo-cellulaires, leur extraction est réalisée par ultra-sons. Le schéma de purification suivi nous a permis d'obtenir six fractions. Trois fractions ne contenant qu'une seule activité : une alpha-neuraminidase et deux beta-galactosidases. Trois fractions possèdent chacune deux activités : une béta-galactosidase et une alpha-fucosidase ; une alpha-neuraminidase et une n-acétyl-béta-hexosaminidase ; une béta-galactosidase et une n-acétyl-béta-hexosaminidase. Toutes ces fractions ne révèlent qu'une seule bande en électrophorèse non dénaturante. Nous avons vérifié que celles-ci correspondaient bien aux activités suivies. Les deux activités béta-galactosidase purifiées séparément sont homogènes en électrophorèse dénaturante. Toutes ces activités ont des températures optimales comprises entre 40 et 48°C. Les pH optimaux sont compris entre 5 et 6. 5 et les masses moléculaires relatives déterminées en gel filtration sur Superose 6 varient de 97000 à 420000. Les pHi sont acides (4 a 5. 1). Seule une activité béta-galactosidase est sensible à l'EDTA et à l'EGTA, de plus Elle est activée par le calcium et le magnésium. Les paramètres enzymatiques des béta-galactosidases ont été réalisés sur le pNP-Gal et sur le lactose, ils mettent en évidence trois groupes de réactivité différente. Tous ces paramètres nous permettent de conclure que Bifido bacterium bifidum souche AA/22 possède au moins trois béta-galactosidases différentes. Nous n'avons pas prouvé que dans les fractions contenant deux activités, nous étions en présence d'un complexe enzymatique. Mais nous avons montré que la présence d'une activité influençait la réactivité de l'autre activité présente.
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Boutry, Etienne. "Exoglycosidases de Bifidobacterium bifidum souche AA 2/2". Lille 1, 1989. http://www.theses.fr/1989LIL10163.

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Abstract (sommario):
La production de beta-d-galactosidase (EC. 3. 2. 1. 23), de n-acetyl-beta-d-glucosaminidase (EC. 3. 2. 1. 30), de neuraminidase (EC. 3. 2. 1. 18) et d'alpha-l-fucosidase (EC. 3. 2. 1. 51) a été optimisée par fermentation. La bactérie anaérobie Bifidobacterium bifidum souche AA/22 a été cultivée en milieu liquide renfermant par litre : 37 g d'un extrait sec de cerveau-cœur, 10 g de glucose et des facteurs bifidigènes (1 g de gynolactose ou 66 ml de lait de femme). La fermentation est conduite sous N2/CO2 à 37°C et à ph 6,8. L'extraction des systèmes enzymatiques par une technique aux ultra-sons a été optimisée en tampon phosphate de sodium 20 mm ph 6,8 renfermant de l'EDTA 20 mm et 0,1 p. 100 de nonidet P 40. Après centrifugation d'une heure à 100 000 g la solution enzymatique obtenue renferme: 7 U de beta-d-galactosidase, 1,6 U de n-acetyl-beta-d-glucosaminidase, 90 mU de neuraminidase et 400 mU de fucosidase par mg de protéine. Une neuraminidase a été purifiée jusqu'à homogénéité en électrophorèse dénaturante (SDS). La masse moléculaire de l'enzyme native est de 75 kDa, elle est constituée par deux sous-unités identiques (35 kDa). Le phi, la température et le ph optimum d'action sont respectivement de 4,2, 50°C et 5,0. Cette neuraminidase est sensible à l'action de la température, elle n'est stable qu'en dessous de 48°C. L'activité est indépendante des métaux. Elle permet l'hydrolyse des liaisons alpha 2,3 plus rapidement que les alpha 2,6 et les alpha 2,8. L'enzyme agit aussi bien sur les glycopeptides que sur les glycoprotéines natives.
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Più fonti

Libri sul tema "Bifidobacterium"

1

Bezkorovainy, Anatoly. Biochemistry and physiology of bifidobacteria. Boca Raton, Fla: CRC Press, 1989.

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2

Maeil Yuŏp Chusik Hoesa. Chungang Yŏnʼguso., a cura di. Hanʼgugin e chŏkhaphan saengni hwalsŏng Bipʻidŏsŭ kyunju ŭi kaebal mit chepʻumhwa =: Development of probiotic Bifidobacteria culture originated from Korean natives and its applications. [Seoul]: Kwahak Kisulbu, 1998.

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3

Satokari, Reetta. Molecular identification and characterisation of bifidobacteria and lactobacilli in the human gastrointestinal tract. Espoo [Finland]: Technical Research Centre of Finland, 2001.

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4

Lifshitz, Ran. Development of DNA probe(s) for the detection of Bifidobacterium spp. in water: Final report. Toronto: Ministry of Environment and Energy, Environmental Research Program, 1996.

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van Sinderen, Douwe, e Marco Ventura, a cura di. Bifidobacteria. New York, NY: Springer US, 2021. http://dx.doi.org/10.1007/978-1-0716-1274-3.

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6

Brandi, G. Bifidobacteria: Microbiological aspects and probiotic potentialities. Milano: Springer-Verlag, 1998.

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7

Koo, Malcolm M. O. Action of bifidobacteria on nitrogen metabolism and colonic carcinogenesis in mice. Ottawa: National Library of Canada, 1990.

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8

Mayo, Baltasar, e Douwe van Sinderen. Bifidobacteria: Genomics and Molecular Aspects. Caister Academic Press, 2010.

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9

Bezkorovainy, Anatoly. Biochemistry and Physiology of Bifidobacteria. Taylor & Francis Group, 2020.

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Bezkorovainy, Anatoly. Biochemistry and Physiology of Bifidobacteria. Taylor & Francis Group, 2020.

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Capitoli di libri sul tema "Bifidobacterium"

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Hoedt, Emily C., Roger S. Bongers, Francesca Bottacini, Jan Knol, John MacSharry e Douwe van Sinderen. "Bifidobacterium Transformation". In Methods in Molecular Biology, 13–19. New York, NY: Springer US, 2021. http://dx.doi.org/10.1007/978-1-0716-1274-3_2.

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2

Sgorbati, B., B. Biavati e D. Palenzona. "The genus Bifidobacterium". In The Genera of Lactic Acid Bacteria, 279–306. Boston, MA: Springer US, 1995. http://dx.doi.org/10.1007/978-1-4615-5817-0_8.

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Lee, J., A. Ametani, Y. Sato e S. Kaminogawa. "Immunopotentiator from Bifidobacterium Adolescentis". In Animal Cell Technology: Basic & Applied Aspects, 155–64. Dordrecht: Springer Netherlands, 1993. http://dx.doi.org/10.1007/978-94-011-2044-9_22.

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Lau, Amy Sie-Yik, Jin-Zhong Xiao e Min-Tze Liong. "Bifidobacterium for Infants: Essence and Efficacy". In Microbiology Monographs, 39–72. Cham: Springer International Publishing, 2015. http://dx.doi.org/10.1007/978-3-319-23213-3_3.

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Obanla, Temitayo O., Saeed A. Hayek, Rabin Gyawali e Salam A. Ibrahim. "Interaction Between Bifidobacterium and Medical Drugs". In Proceedings of the 2013 National Conference on Advances in Environmental Science and Technology, 171–78. Cham: Springer International Publishing, 2016. http://dx.doi.org/10.1007/978-3-319-19923-8_17.

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Ruiz, Lorena, e Douwe van Sinderen. "Implementation of Transposon Mutagenesis in Bifidobacterium". In Microbial Transposon Mutagenesis, 51–62. New York, NY: Springer New York, 2019. http://dx.doi.org/10.1007/978-1-4939-9570-7_5.

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Mattarelli, Paola, e Bruno Biavati. "The genera Bifidobacterium, Parascardovia and Scardovia". In Lactic Acid Bacteria, 509–41. Chichester, UK: John Wiley & Sons, Ltd, 2014. http://dx.doi.org/10.1002/9781118655252.ch29.

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James, Kieran, e Douwe van Sinderen. "Site-Directed Mutagenesis of Bifidobacterium Strains". In Methods in Molecular Biology, 45–60. New York, NY: Springer US, 2021. http://dx.doi.org/10.1007/978-1-0716-1274-3_5.

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Jiménez, Esther, Marta Gómez e Laura Moles. "Pulsed Field Gel Electrophoresis for Bifidobacterium". In Methods in Molecular Biology, 253–63. New York, NY: Springer New York, 2015. http://dx.doi.org/10.1007/978-1-4939-2599-5_20.

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Radhamanalan, Guhanraj, e Dhanasekaran Dharumadurai. "Preparation of Postbiotics from Bifidobacterium sp." In Methods and Protocols in Food Science, 51–54. New York, NY: Springer US, 2023. http://dx.doi.org/10.1007/978-1-0716-3421-9_6.

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Atti di convegni sul tema "Bifidobacterium"

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Zoruk, P. Yu, D. Y. Boldireva, E. I. Olekhnovich e K. M. Klimina. "PHARMACOKINETIC STUDY OF LACTOBACILLI AND BIFIDOBACTERIA STRAINS IN MICE". In OpenBio-2023. ИПЦ НГУ, 2023. http://dx.doi.org/10.25205/978-5-4437-1526-1-18.

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Abstract (sommario):
Immunological approaches have demonstrated high efficiency in the fight against various types of malignant tumors. However, some patients do not form the expected positive response to immunotherapy. Among other factors, this effect is also associated with the composition of intestinal microbiota [1–3]. Based on previously analyzed data, taxonomic groups of bacteria associated with a positive response to cancer immunotherapy were identified, in particular bacteria of genus Bifidobacterium adolescentis. In a pilot experiment on laboratory mice (females, С57Bl/6) we studied the pharmacokinetics of excretion of lactobacilli and bifidobacteria strains in mice and biological diversity in fecal samples.
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He, Chen, Hu Man, Shu Guowei, Ma Qi e Qin Tao. "Effect of prebiotics on growth of bifidobacterium bifidum". In 2011 International Conference on Human Health and Biomedical Engineering (HHBE). IEEE, 2011. http://dx.doi.org/10.1109/hhbe.2011.6028988.

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Holt, Scott M., Chris Skory e Greg Cote. "Growth Assessment of Bifidobacterium on Glucansucrase-Derived Oligosaccharides". In 1st International Electronic Conference on Microbiology. Basel, Switzerland: MDPI, 2020. http://dx.doi.org/10.3390/ecm2020-07135.

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Milentyeva, Irina, Anastasiya Fedorova e Yu A. Erofeeva. "RESEARCH AND DEVELOPMENT OF PROBIOTIC SUPPLEMENTS BASED ON METABOLITES OF BIFIDOBACTERIUM AND LACTOBACILLUS BACTERIA". In I International Congress “The Latest Achievements of Medicine, Healthcare, and Health-Saving Technologies”. Kemerovo State University, 2023. http://dx.doi.org/10.21603/-i-ic-89.

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Villanueva-Gudiño, F., E. Borrayo e C. Gómez-Márquez. "Unraveling GABA-Related Metabolic Pathways in Diverse Bifidobacterium Species". In 2023 IEEE EMBS R9 Conference. IEEE, 2023. http://dx.doi.org/10.1109/ieeeconf60929.2023.10525637.

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Silva, Luana Sabrine, Ana Cláudia Morais Souza, Marcelo Morais Maria, Annatachi Botelho Jardim, Washington Azevedo da Silva, Christiano Vieira Pires, Marcelo Resende Souza e Andréia Marçal da Silva. "Estudo da Viabilidade de Bifidobacterium Longum em Bebidas Lácteas Probióticas". In XII Latin American Congress on Food Microbiology and Hygiene. São Paulo: Editora Edgard Blücher, 2014. http://dx.doi.org/10.5151/foodsci-microal-155.

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Silva, Luana Sabrine, Ana Cláudia Morais Souza, Marcelo Morais Maria, Cláudia Freire de Andrade Morais Penna, Washington Azevedo da Silva, Christiano Vieira Pires, Marcelo Resende Souza e Andréia Marçal da Silva. "Avaliação Microbiológica e Físico-Química de Queijos Probióticos Contendo Bifidobacterium Longum". In XII Latin American Congress on Food Microbiology and Hygiene. São Paulo: Editora Edgard Blücher, 2014. http://dx.doi.org/10.5151/foodsci-microal-157.

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Bessell, Catherine Ami. "Abstract B060: Commensal bacteria Bifidobacterium stimulates an antitumor response via cross-reactivity". In Abstracts: Fourth CRI-CIMT-EATI-AACR International Cancer Immunotherapy Conference: Translating Science into Survival; September 30 - October 3, 2018; New York, NY. American Association for Cancer Research, 2019. http://dx.doi.org/10.1158/2326-6074.cricimteatiaacr18-b060.

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Dorta, Claudia, Anna Claudia Sahade Brunatti, Flávia Maria Vasques Farinazzi-Machado, Vanessa Pachelle Simão e Ariele Cristina Viana dos Santos. "Sorvete Sabor Morango com Adição do Probiótico Bifidobacterium Bifidum ou Lactobacillus Casei". In XII Latin American Congress on Food Microbiology and Hygiene. São Paulo: Editora Edgard Blücher, 2014. http://dx.doi.org/10.5151/foodsci-microal-001.

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Carvalho, Jorge Luis, Ana Karolina Sá, Aurileia Britto, Munique Ferreira, Elen Anatriello, Alexandre Keller e Flavio Aimbire. "Bifidobacterium breve significantly reduces cigarette smoke-induced COPD in C57Bl/6 mice". In ERS International Congress 2018 abstracts. European Respiratory Society, 2018. http://dx.doi.org/10.1183/13993003.congress-2018.pa4244.

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Rapporti di organizzazioni sul tema "Bifidobacterium"

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Irkin, Reyhan. The Survival of Bifidobacterium bifidum NRRL B41410 in Traditional Beverage: Shalgam. "Prof. Marin Drinov" Publishing House of Bulgarian Academy of Sciences, settembre 2018. http://dx.doi.org/10.7546/crabs.2018.09.18.

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Stifeev, A. I., V. I. Lazarev e O. V. Nikitina. NEW APPROACHES TO THE DEVELOPMENT OF A BIOLOGICALLY ACTIVE SUPPLEMENT BASED ON BIFIDOBACTERIUM BIFIDUM METABOLITES. FGBOU VO Kursk State Agricultural Academy, Journal Bulletin of the Kursk State Agricultural Academy., 2020. http://dx.doi.org/10.18411/issn1997-0749.2020-05-18.

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XU, QIANWEN, e QIKUN HUANG. Meta-analysis of the clinical efficacy of Bifidobacterium trivium in the treatment of asthma in children. INPLASY - International Platform of Registered Systematic Review and Meta-analysis Protocols, maggio 2022. http://dx.doi.org/10.37766/inplasy2022.5.0022.

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Li, Shuyao, Wenshuang Zhang e Tong Guan. Effect of Bifidobacterium Bifidum for Chronic Obstructive Pulmonary Disease in China: A Systematic Review and Meta-Analysis. INPLASY - International Platform of Registered Systematic Review and Meta-analysis Protocols, giugno 2022. http://dx.doi.org/10.37766/inplasy2022.6.0023.

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Yang, Yi, Lubo Shi, Xiaoduo Liu e Anni Zhou. Effects of Bifidobacterium on patients with irritable bowel syndrome: a systematic review and meta‑analysis of randomized clinical trials. INPLASY - International Platform of Registered Systematic Review and Meta-analysis Protocols, settembre 2023. http://dx.doi.org/10.37766/inplasy2023.9.0096.

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Multi-laboratory study and interlaboratory study on the enumeration of bifidobacteria for in milk products. International Dairy Federation (IDF) AISBL, 2024. http://dx.doi.org/10.56169/tdtn3840.

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