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Letteratura scientifica selezionata sul tema "Bacteriophages Genetics"

Autore: Grafiati

Pubblicato: 4 giugno 2021

Ultima modifica: 28 gennaio 2023

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Articoli di riviste sul tema "Bacteriophages Genetics"

1

Auslander, Noam, Ayal B. Gussow, Sean Benler, Yuri I. Wolf e Eugene V. Koonin. "Seeker: alignment-free identification of bacteriophage genomes by deep learning". Nucleic Acids Research 48, n. 21 (12 ottobre 2020): e121-e121. http://dx.doi.org/10.1093/nar/gkaa856.

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Abstract Recent advances in metagenomic sequencing have enabled discovery of diverse, distinct microbes and viruses. Bacteriophages, the most abundant biological entity on Earth, evolve rapidly, and therefore, detection of unknown bacteriophages in sequence datasets is a challenge. Most of the existing detection methods rely on sequence similarity to known bacteriophage sequences, impeding the identification and characterization of distinct, highly divergent bacteriophage families. Here we present Seeker, a deep-learning tool for alignment-free identification of phage sequences. Seeker allows rapid detection of phages in sequence datasets and differentiation of phage sequences from bacterial ones, even when those phages exhibit little sequence similarity to established phage families. We comprehensively validate Seeker's ability to identify previously unidentified phages, and employ this method to detect unknown phages, some of which are highly divergent from the known phage families. We provide a web portal (seeker.pythonanywhere.com) and a user-friendly Python package (github.com/gussow/seeker) allowing researchers to easily apply Seeker in metagenomic studies, for the detection of diverse unknown bacteriophages.

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2

Timmons, Michael S., M. Lieb e Richard C. Deonier. "RECOMBINATION BETWEEN IS5 ELEMENTS: REQUIREMENT FOR HOMOLOGY AND RECOMBINATION FUNCTIONS". Genetics 113, n. 4 (1 agosto 1986): 797–810. http://dx.doi.org/10.1093/genetics/113.4.797.

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ABSTRACT Intermolecular recombination between two IS5 elements was measured, using bacteriophage lambda recombination vectors, and was compared to recombination between two copies of an SV40 segment cloned into the same vectors. Experiments were conducted in the presence and in the absence of RecA and Red functions, and with the recombining inserts in the same or in reversed orientation. Under all conditions, IS5 elements recombined in a manner similar to the SV40 inserts, indicating that IS-encoded functions did not confer measurable additional intermolecular recombination ability to IS5 in E. coli K-12. Bacteriophages containing reversed IS5 inserts, for which the 16 base pair (bp) termini are identical in 15 positions and which display 12 bp of uninterrupted homology, recombined at approximately the same low frequency under Rec+ and Rec- conditions, indicating that these short homologies were not good substrates for the Rec system. Bacteriophages having reversed inserts recombined better under Red+ than under Red- conditions, but the crossovers were located in nonhomologous regions flanking the element termini. This suggests that 12-bp homologies are not good substrates for the Red system.

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3

Węgrzyn, Grzegorz. "Should Bacteriophages Be Classified as Parasites or Predators?" Polish Journal of Microbiology 71, n. 1 (23 febbraio 2022): 3–9. http://dx.doi.org/10.33073/pjm-2022-005.

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Abstract Bacteriophages are viruses infecting bacteria and propagating in bacterial cells. They were discovered over 100 years ago, and for decades they played crucial roles as models in genetics and molecular biology and as tools in genetic engineering and biotechnology. Now we also recognize their huge role in natural environment and their importance in human health and disease. Despite our understanding of bacteriophage mechanisms of development, these viruses are described as parasites or predators in the literature. From the biological point of view, there are fundamental differences between parasites and predators. Therefore, in this article, I asked whether bacteriophages should be classified as former or latter biological entities. Analysis of the literature and biological definitions led me to conclude that bacteriophages are parasites rather than predators and should be classified and described as such. If even more precise ecological classification is needed, bacteriophages can perhaps be included in the group of parasitoids. It might be the most appropriate formal classification of these viruses, especially if strictly virulent phages are considered, contrary to phages which lysogenize host cells and those which develop according to the permanent infection mode (or chronic cycle, like filamentous phages) revealing features of classical parasites.

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4

Schrader, Holly S., John O. Schrader, Jeremy J. Walker, Thomas A. Wolf, Kenneth W. Nickerson e Tyler A. Kokjohn. "Bacteriophage infection and multiplication occur inPseudomonas aeruginosastarved for 5 years". Canadian Journal of Microbiology 43, n. 12 (1 dicembre 1997): 1157–63. http://dx.doi.org/10.1139/m97-164.

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Bacteriophages specific for Pseudomonas aeruginosa and Escherichia coli were examined for their ability to multiply in stationary phase hosts. Four out of five bacteriophages tested, including E. coli bacteriophage T7M, were able to multiply in stationary phase hosts. The bacteriophage ACQ had a mean burst size of approximately 1000 in exponential phase P. aeruginosa hosts and 102 in starved hosts, with corresponding latent periods that increased from 65 to 210 min. The bacteriophage UT1 had a mean burst size of approximately 211 in exponential phase P. aeruginosa hosts and 11 in starved hosts, with latent periods that increased from a mean of 90 min in exponential phase hosts to 165 min in starved hosts. Bacteriophage multiplication occurred whether or not the hosts had entered stationary phase, either because the cultures had been incubated for 24 h or were starved. Significantly, bacteriophage multiplication occurred in P. aeruginosa, which had been starved for periods of 24 h, several weeks, or 5 years. Only one P. aeruginosa virus, BLB, was found to be incapable of multiplication in stationary phase hosts. These results reveal that starvation does not offer bacterial hosts refuge from bacteriophage infection and suggest that bacteriophages will be responsible for significant bacterial mortality in most natural ecosystems.Key words: bacteriophage multiplication, stationary phase, starvation.

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5

Klein, Gracjana, e Costa Georgopoulos. "Identification of Important Amino Acid Residues That Modulate Binding of Escherichia coli GroEL to Its Various Cochaperones". Genetics 158, n. 2 (1 giugno 2001): 507–17. http://dx.doi.org/10.1093/genetics/158.2.507.

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Abstract Genetic experiments have shown that the GroEL/GroES chaperone machine of Escherichia coli is absolutely essential, not only for bacterial growth but also for the propagation of many bacteriophages including λ. The virulent bacteriophages T4 and RB49 are independent of the host GroES function, because they encode their own cochaperone proteins, Gp31 and CocO, respectively. E. coli groEL44 mutant bacteria do not form colonies above 42° nor do they propagate bacteriophages λ, T4, or RB49. We found that the vast majority (40/46) of spontaneous groEL44 temperature-resistant colonies at 43° were due to the presence of an intragenic suppressor mutation. These suppressors define 21 different amino acid substitutions in GroEL, each affecting one of 13 different amino acid residues. All of these amino acid residues are located at or near the hinge, which regulates the large en bloc movements of the GroEL apical domain. All of these intragenic suppressors support bacteriophages λ, T4, and RB49 growth to various extents in the presence of the groEL44 allele. Since it is known that the GroEL44 mutant protein does not interact effectively with Gp31, the suppressor mutations should enhance cochaperone binding. Analogous intragenic suppressor studies were conducted with the groEL673 temperature-sensitive allele.

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6

Brüggemann, Holger, e Rolf Lood. "Bacteriophages InfectingPropionibacterium acnes". BioMed Research International 2013 (2013): 1–10. http://dx.doi.org/10.1155/2013/705741.

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Viruses specifically infecting bacteria, or bacteriophages, are the most common biological entity in the biosphere. As such, they greatly influence bacteria, both in terms of enhancing their virulence and in terms of killing them. Since the first identification of bacteriophages in the beginning of the 20th century, researchers have been fascinated by these microorganisms and their ability to eradicate bacteria. In this review, we will cover the history of thePropionibacterium acnesbacteriophage research and point out how bacteriophage research has been an important part of the research onP. acnesitself. We will further discuss recent findings from phage genome sequencing and the identification of phage sequence signatures in clustered regularly interspaced short palindromic repeats (CRISPRs). Finally, the potential to useP. acnesbacteriophages as a therapeutic strategy to combatP. acnes-associated diseases will be discussed.

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7

Benedi, Vicente J., Miguel Regué, Sebastián Albertí, Silvia Camprubí e Juan M. Tomás. "Influence of environmental conditions on infection of Klebsiella pneumoniae by two different types of bacteriophages". Canadian Journal of Microbiology 37, n. 4 (1 aprile 1991): 270–75. http://dx.doi.org/10.1139/m91-042.

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The adsorption and efficiency of plating of bacteriophages FC3-1 and FC3-9 on Klebsiella pneumoniae C3 (serotype O1:K66) cells grown at different pHs and temperatures were quantitated. Bacteriophage FC3-1, with lipopolysaccharide as its bacterial receptor, showed a large decrease in efficiency of plating on bacteria grown at low pH or low temperature. Under the same conditions, no significant decrease in efficiency of plating was found for bacteriophage FC3-9, a phage requiring capsule and lipopolysaccharide for its adsorption and carrying capsule-depolymerizing activity. We demonstrate that K. pneumoniae C3 cells grown at low pH or low temperature have less lipopolysaccharide exposed on their surface. We conclude that this is why lipopolysaccharide-specific phage FC3-1 less efficiently infects bacterial cells grown under those conditions. We propose that bacteriophage FC3-9 efficiently infects bacterial cells grown at low pH or low temperature because its enzymatic activity on the capsule makes lipopolysaccharide available to this phage. Key words: Klebsiella pneumoniae, bacteriophages, isolation, environmental conditions.

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8

Verbeken, Gilbert, Isabelle Huys, Jean-Paul Pirnay, Serge Jennes, Nina Chanishvili, Jacques Scheres, Andrzej Górski, Daniel De Vos e Carl Ceulemans. "Taking Bacteriophage Therapy Seriously: A Moral Argument". BioMed Research International 2014 (2014): 1–8. http://dx.doi.org/10.1155/2014/621316.

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The excessive and improper use of antibiotics has led to an increasing incidence of bacterial resistance. In Europe the yearly number of infections caused by multidrug resistant bacteria is more than 400.000, each year resulting in 25.000 attributable deaths. Few new antibiotics are in the pipeline of the pharmaceutical industry. Early in the 20th century, bacteriophages were described as entities that can control bacterial populations. Although bacteriophage therapy was developed and practiced in Europe and the former Soviet republics, the use of bacteriophages in clinical setting was neglected in Western Europe since the introduction of traditional antibiotics. Given the worldwide antibiotic crisis there is now a growing interest in making bacteriophage therapy available for use in modern western medicine. Despite the growing interest, access to bacteriophage therapy remains highly problematic. In this paper, we argue that the current state of affairs is morally unacceptable and that all stakeholders (pharmaceutical industry, competent authorities, lawmakers, regulators, and politicians) have the moral duty and the shared responsibility towards making bacteriophage therapy urgently available for all patients in need.

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9

Gong, Chao, Spencer Heringa, Randhir Singh, Jinkyung Kim e Xiuping Jiang. "Isolation and characterization of bacteriophages specific to hydrogen-sulfide-producing bacteria". Canadian Journal of Microbiology 59, n. 1 (gennaio 2013): 39–45. http://dx.doi.org/10.1139/cjm-2012-0245.

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The objectives of this study were to isolate and characterize bacteriophages specific to hydrogen-sulfide-producing bacteria (SPB) from raw animal materials, and to develop a SPB-specific bacteriophage cocktail for rendering application. Meat, chicken offal, and feather samples collected from local supermarkets and rendering processing plants were used to isolate SPB (n = 142). Bacteriophages (n = 52) specific to SPB were isolated and purified from the above samples using 18 of those isolated SPB strains as hosts. The host ranges of bacteriophages against 5 selected SPB strains (Escherichia coli, Citrobacter freundii, and Hafnia alvei) were determined. Electron microscopy observation of 9 phages selected for the phage cocktail revealed that 6 phages belonged to the family of Siphoviridae and 3 belonged to the Myoviridae family. Restriction enzyme digestion analysis with endonuclease DraI detected 6 distinguished patterns among the 9 phages. Phage treatment prevented the growth of SPB for up to 10 h with multiplicity of infection ratios of 1, 10, 100, and 1000 in tryptic soy broth at 30 °C, and extended the lag phase of SPB growth for 2 h at 22 °C with multiplicities of infection of 10, 100, and 1000. These results suggest that the selected bacteriophage cocktail has a high potential for phage application to control SPB in raw animal materials destined for the rendering process.

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Jończyk-Matysiak, Ewa, Marlena Kłak, Beata Weber-Dąbrowska, Jan Borysowski e Andrzej Górski. "Possible Use of Bacteriophages Active againstBacillus anthracisand OtherB. cereusGroup Members in the Face of a Bioterrorism Threat". BioMed Research International 2014 (2014): 1–14. http://dx.doi.org/10.1155/2014/735413.

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Anthrax is an infectious fatal disease with epidemic potential. Nowadays, bioterrorism usingBacillus anthracisis a real possibility, and thus society needs an effective weapon to neutralize this threat. The pathogen may be easily transmitted to human populations. It is easy to store, transport, and disseminate and may survive for many decades. Recent data strongly support the effectiveness of bacteriophage in treating bacterial diseases. Moreover, it is clear that bacteriophages should be considered a potential incapacitative agent against bioterrorism using bacteria belonging toB. cereusgroup, especiallyB. anthracis. Therefore, we have reviewed the possibility of using bacteriophages active againstBacillus anthracisand other species of theB. cereusgroup in the face of a bioterrorism threat.

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Tesi sul tema "Bacteriophages Genetics"

1

Dibbens, Justin Andrew. "Studies on the control of late gene transcription in coliphage 186 /". Title page, contents and summary only, 1990. http://web4.library.adelaide.edu.au/theses/09PH/09phd543.pdf.

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2

Brathwaite, Kelly Janelle. "Interactions between Campylobacters and their bacteriophages". Thesis, University of Nottingham, 2015. http://eprints.nottingham.ac.uk/28422/.

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Campylobacter jejuni is a leading cause of human bacterial enteritis worldwide. Consumption of contaminated poultry meat is considered a major source of infection. The use of virulent bacteriophages as a form of biocontrol to specifically reduce this pathogen in poultry (phage therapy) is a promising intervention that does not rely on antimicrobials and therefore circumvents the emergence of antibiotic-resistant Campylobacter strains. In order to achieve this, a better understanding of the mechanisms involved in phage-host interactions at the molecular level would assist in the development of the strategy and the selection of bacteriophages. The main objective of this study was to therefore examine such interactions between Campylobacter and its virulent phages. To achieve this, the transcriptional response of C. jejuni to phage infection was investigated, along with the role of a Type II restriction-modification system during phage infection of Campylobacter. These studies were conducted using the highly phage-sensitive Campylobacter strain, C. jejuni PT14, in conjunction with a number of group II and III bacteriophages (Eucampyvirinae). Transcriptome studies (RNA-Seq) revealed a phage-induced host response that included a demand for iron and oxygen. This was highlighted by the up-regulation of several siderophore-based iron acquisition genes and down-regulation of genes associated with a number of anaerobic electron transport pathways that utilise alternative electron acceptors to oxygen. In addition, the pattern of gene regulation also suggested apo-Fur regulation of the iron-responsive and flagellar biogenesis genes. This host response has been proposed to occur as a consequence of the reduction of ribonucleotides to form deoxyribonucleotides during phage DNA replication. This process is catalysed by the enzyme ribonucleotide reductase and requires iron and oxygen during the formation of a reactive di-iron centre within the β-subunit of the enzyme. Unusually knock-out mutants of a Type II restriction-modification system had a negative impact on phage replication. The A911_00150 mutant displayed pleiotropic changes in motility, cell based invasion and the ability to colonise chickens. Transcriptome analysis highlighted down-regulation of the genes required for the synthesis of the bacterial flagellum.

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3

Mmolawa, Princess Tlou. "Molecular analysis of temperate phages in Salmonella enterica serovar Typhimurium DT 64 isolated in Australia". Title page, contents and summary only, 2001. http://web4.library.adelaide.edu.au/theses/09PH/09phm6855.pdf.

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Files on accompanying CD-ROM: Appendix III Phages ST64T and ST64B sequences, are in rtf format. Bibliography: leaves 279-324. System requirements for accompanying CD-ROM: IBM or compatible ; Microsoft Word or compatible to read rtf files.

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GARVEY, KEVIN JAMES. "DNA SEQUENCE ANALYSIS OF BACILLUS PHAGE PHI29 RIGHT EARLY REGION AND LATE GENES 14, 15 AND 16 (LYSOZYME)". Diss., The University of Arizona, 1986. http://hdl.handle.net/10150/183839.

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The sequence of the rightmost 4,626 bp of the Bacillus phage φ29 genome is presented and analyzed. Nine large open reading frames (ORF's) have been found. Three of these ORF's are correlated with the late genes 14, 15 and 16. The remaining six ORF's are in the right early region. One of these early ORF's has been identified as gene 17 (g17), the only early gene to have been genetically mapped in this region. The remaining ORF's (16.5, 16.6, 16.7, 16.8 and 16.9) were previously unknown. The biological efficacies of some of these putative early ORF's were demonstrated using an in vitro E. coli transcription-translation system. The primary amino acid sequences, molecular weights, translational initiation sequences and genetic organization of these nine genes are presented and discussed. Gene product 15 (gp15) was found to have strong homology with Salmonella phage P22 gp19, a lysozyme. gp15 also has a lesser but possibly significant homology with T4 gene product e (gpe), also a lysozyme. Using a clone containing φ29 g15 it was shown that gp15 can complement T4 gene e (ge) mutant infections, leading to the conclusion that φ29 g15 encodes a lysozyme. Three transcriptional initiation sites (P(E)3, P(EC)3 and B2) were previously mapped in this region. The sequences of the putative P(EC)3 and B2 promoter sites are presented and shown to have homology with the Bacillus σ⁵⁵ concensus sequence. Sequences having homology to a minor Bacillus sigma factor recognition site, σ³², are also presented and discussed. The region between the last late gene (g16) and the last early gene (ORF-16.5) consists of only 30 bp. Analysis of potential secondary structures of transcripts across this region suggests that the same sequences may be involved in the termination of both late and early transcription.

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Goh, Shan. "Phenotypic and genotypic characterisation of bacteriophages of Clostridium difficile". University of Western Australia. Microbiology Discipline Group, 2003. http://theses.library.uwa.edu.au/adt-WU2004.0018.

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Clostridium difficile is an important hospital-acquired pathogen causing C. difficile-associated diarrhoea (CDAD) in patients exposed to antibiotics. The lack of information on bacteriophages of C. difficile, and the potential of phages as therapeutic agents for the treatment of CDAD, prompted the isolation and characterisation of phages active against clinical isolates of C. difficile in order to determine the prevalence and significance of phages of this anaerobe. Three (5.4 %) of 56 clinical C. difficile isolates induced by mitomycin C yielded dsDNA phages C2, C5, C6 and C8. The four phages differed from previously described C. difficile phages in particle morphology, burst size and host range. C2, C5 and C8 particles were members of the family Myoviridae, while C6 belonged to Siphoviridae. The burst sizes were 5 for C2, 7 for C5, 19 for C6 and 33 for C8. C8 had the broadest host range, lysing 27 out of 56 (48 %) C. difficile isolates, followed by C6 (43 %), C5 (20 %) and C2 (20 %). Superinfection experiments, restriction enzyme analysis and Southern hybridisation showed C2 and C5 to be closely related with C8 somewhat related to them, however, C6 was distantly related to the other three phages. C2 was further characterised as a representative phage. Its genome did not possess cohesive ends, and was shown to integrate chromosomally via an attP site identified within a 1.9 kb HindIII fragment. However, an integrase gene, which is typically close to the attP region, was not located. Nine of 16 HindIII fragments of C2, including the 1.9 kb fragment, were cloned into pUC18. Approximately 9 kb of the estimated 43 kb genome of C2 was sequenced and analysed. Seven of the nine translated sequences were homologous to phage structural proteins, two sequences were not homologous to any relevant protein in the Genbank and EMBL databases, and one was homologous to proteins of Clostridium species. Nucleotide homology between the C2 sequences and the recently sequenced C. difficile strain CD630 was found in three regions within CD630 genome. Seven of the nine sequences, including the 1.9 kb fragment, were clustered in one region. These data suggest that the genes constitute a phage structural gene module. The presence of C2-like sequences in CD630, and Southern hybridisation of C. difficile strains using phage probes, suggested related prophage sequences may be commonly present in this bacterial species. An investigation was carried out to determine the presence of toxin genes tcdA and tcdB, and PaLoc-associated gene tcdE, in phage DNA. In addition, the effect of phage infection on toxin production of toxigenic C. difficile strains was studied. Of the three genes, tcdE only was detected in phages C2, C5 and C8, but not in C6. Strains that maintained phages in a stable manner (lysogens) were isolated and used in toxin studies. The amount of toxin B produced was measured by cytotoxic assays using Vero cells, and toxin A production was measured by ELISA. Although phages did not encode toxin A or B genes, there was a significant increase in toxin B production in some lysogens. There was no increase in toxin A production. Transcriptional analyses of tcdA and tcdB in lysogens and parental strains was performed by real-time RT-PCR and Northern hybridisation to determine whether phage was affecting regulation of toxin transcription. Phage did not appear to affect toxin gene transcription, although results from real-time RT-PCR and Northern hybridisation were conflicting. A phage induced from the highly toxigenic reference strain VPI 10463 was also briefly characterised and investigated for its effect on toxin production in VPI 10463. The phage, ΦCV, had similar particle morphology to C2, C5 and C8, and had some HindIII bands in common with C2 and C5. Two cured variant strains produced significantly less toxin B compared to VPI 10463. In conclusion, several important properties of C. difficile phages were characterised. It appears these temperate phages may play a role in toxin production making them unsuitable as therapeutic agents for the treatment of CDAD. However, C2 phage may have potential as the basis for an integrative vector that will add to the genetic tools available for clostridia.

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Harrison, Sharon Jane. "Targeted transgenesis and the 186 site-specific recombination system /". Title page, summary and contents only, 1999. http://web4.library.adelaide.edu.au/theses/09PH/09phh322.pdf.

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7

Huen, Shing-yan Michael, e 禤承恩. "A mechanistic study of lambdaphage-mediated recombination in E. coli". Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2006. http://hub.hku.hk/bib/B35321854.

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Swanson, Rhett. "Cloning and expression of the genes encoding bacteriophage T7 & SP6 RNA polymerase /". Title page, table of contents and summary only, 1990. http://web4.library.adelaide.edu.au/theses/09PH/09phs9722.pdf.

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Chang, Jenny Ren-Jye. "Scaffolding-mediated capsid size determination in bacteriophages". Birmingham, Ala. : University of Alabama at Birmingham, 2009. https://www.mhsl.uab.edu/dt/2009p/changj.pdf.

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Thesis (Ph. D.)--University of Alabama at Birmingham, 2009.
Title from PDF title page (viewed Jan. 26, 2010). Additional advisors: Asim K. Bej, Gail E. Christie, Peter E. Prevelige, Jr., R. Douglas Watson. Includes bibliographical references.

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Brumby, Anthony Mansfield. "The control of prophage induction in coliphage 186 /". Title page, contents and summary only, 1994. http://web4.library.adelaide.edu.au/theses/09PH/09phb893.pdf.

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Libri sul tema "Bacteriophages Genetics"

1

Toompuu, O. G. Geneticheskai͡a︡ rekombinat͡s︡ii͡a︡ u bakteriofagov. Tallin: "Valgus", 1985.

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2

Bross, Peter. Untersuchungen zu Struktur und Funktion der Gen 3 Proteine der evolutionär verwandten filamentösen Bakteriophagen IKe und fd. Konstanz: Hartung-Gorre, 1987.

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3

Wendy, Champness, a cura di. Molecular genetics of bacteria. Washington, D.C: ASM Press, 1997.

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4

Bacterial and bacteriophage genetics. 4^a ed. New York: Springer, 2000.

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Bacterial and bacteriophage genetics. 3^a ed. New York: Springer-Verlag, 1994.

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6

Molecular genetics of Escherichia coli. New York, NY: Guilford Press, 1989.

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Bacterial and bacteriophage genetics: An introduction. 2^a ed. New York: Springer-Verlag, 1988.

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T, Adams Horace, a cura di. Contemporary trends in bacteriophage research. New York: Nova Science Publishers, 2009.

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9

C, Summers William, a cura di. Reconceiving the gene: Seymour Benzer's adventures in phage genetics. New Haven: Yale University Press, 2006.

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10

Rosander, Anna. Novel applications of shotgun phage display. Uppsala: Swedish University of Agricultural Sciences, 2004.

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Capitoli di libri sul tema "Bacteriophages Genetics"

1

Klaenhammer, T. R., e G. F. Fitzgerald. "Bacteriophages and bacteriophage resistance". In Genetics and Biotechnology of Lactic Acid Bacteria, 106–68. Dordrecht: Springer Netherlands, 1994. http://dx.doi.org/10.1007/978-94-011-1340-3_3.

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2

Casey, Aidan, Aidan Coffey e Olivia McAuliffe. "Genetics and Genomics of Bacteriophages". In Bacteriophages, 1–26. Cham: Springer International Publishing, 2017. http://dx.doi.org/10.1007/978-3-319-40598-8_5-1.

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3

Casey, Aidan, Aidan Coffey e Olivia McAuliffe. "Genetics and Genomics of Bacteriophages". In Bacteriophages, 193–218. Cham: Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-319-41986-2_5.

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4

Bainbridge, Brian W. "Genetic Analysis of Bacteriophages". In Genetics of Microbes, 69–90. Boston, MA: Springer US, 1987. http://dx.doi.org/10.1007/978-1-4615-7093-6_4.

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Bainbridge, Brian W. "Genetic Analysis of Bacteriophages". In Genetics of Microbes, 69–90. Boston, MA: Springer US, 1987. http://dx.doi.org/10.1007/978-1-4615-7096-7_4.

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6

Birge, Edward A. "Genetics of Temperate Bacteriophages". In Bacterial and Bacteriophage Genetics, 154–81. New York, NY: Springer New York, 1988. http://dx.doi.org/10.1007/978-1-4757-1995-6_6.

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7

Birge, Edward A. "Genetics of Temperate Bacteriophages". In Bacterial and Bacteriophage Genetics, 206–39. New York, NY: Springer New York, 1994. http://dx.doi.org/10.1007/978-1-4757-2328-1_8.

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8

Birge, Edward A. "Genetics of Temperate Bacteriophages". In Bacterial and Bacteriophage Genetics, 253–92. New York, NY: Springer New York, 2000. http://dx.doi.org/10.1007/978-1-4757-3258-0_8.

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9

Birge, Edward A. "Genetics of Other Intemperate Bacteriophages". In Bacterial and Bacteriophage Genetics, 124–53. New York, NY: Springer New York, 1988. http://dx.doi.org/10.1007/978-1-4757-1995-6_5.

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10

Birge, Edward A. "Genetics of Other Intemperate Bacteriophages". In Bacterial and Bacteriophage Genetics, 174–205. New York, NY: Springer New York, 1994. http://dx.doi.org/10.1007/978-1-4757-2328-1_7.

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Atti di convegni sul tema "Bacteriophages Genetics"

1

Самойлова, Анна. "Бактериофаги Pseudomonas syringae pv. syringae перспективные в подавлении развития бактериального рака плодовых". In VIIth International Scientific Conference “Genetics, Physiology and Plant Breeding”. Institute of Genetics, Physiology and Plant Protection, Republic of Moldova, 2021. http://dx.doi.org/10.53040/gppb7.2021.88.

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Abstract (sommario):

Five Pseudomonas syringae pv. syringae bacteriophages were isolated from the quince, apple and pear. After a detailed study, the isolated bacteriophages could be used for biocontrol of the bacterial canker patho-gen. One of the isolated phages was active against the causative agents of bacterial canker and fire blight.

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2

"Plant VLP production system based on bacteriophage MS2 coat protein". In Plant Genetics, Genomics, Bioinformatics, and Biotechnology. Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, 2019. http://dx.doi.org/10.18699/plantgen2019-021.

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3

Orlovskaya, P. I., T. A. Pilipchuk, N. I. Girilovich, M. N. Mandrik-Litvinkovich e E. I. Kalamiyets. "Investigation of genetic heterogeneity of phages from phytopathogenic bacteria Xanthomonas phaseoli". In 2nd International Scientific Conference "Plants and Microbes: the Future of Biotechnology". PLAMIC2020 Organizing committee, 2020. http://dx.doi.org/10.28983/plamic2020.188.

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4

Li, J. K., C. Sun, S. Li, Y. S. Cui, Y. L. Wei e X. L. Ji. "Genetic Diversity of Bacteriophage Communities in Napahai Wetland". In AASRI International Conference on Industrial Electronics and Applications (IEA 2015). Paris, France: Atlantis Press, 2015. http://dx.doi.org/10.2991/iea-15.2015.169.

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5

Ataee, Shabnam, Oscar Rodriguez, Xavier Brochet e Carlos Andres Pena. "Towards BacterioPhage Genetic Edition: Deep Learning Prediction of Phage-Bacterium Interactions". In 2020 IEEE International Conference on Bioinformatics and Biomedicine (BIBM). IEEE, 2020. http://dx.doi.org/10.1109/bibm49941.2020.9313487.

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6

Hirsh, Andrew D., Todd D. Lillian e N. C. Perkins. "A Model for Highly Strained DNA in a Cavity". In ASME 2011 International Design Engineering Technical Conferences and Computers and Information in Engineering Conference. ASMEDC, 2011. http://dx.doi.org/10.1115/detc2011-48711.

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Abstract (sommario):

A single DNA molecule is a long and flexible biopolymer that contains the genetic code. Building upon the discovery of the iconic double helix over 50 years ago, subsequent studies have emphasized how its biological function is related to the mechanical properties of the molecule. A remarkable system which high-lights the role of DNA bending and twisting is the packing and ejection of DNA into and from viral capsids. A recent 3D reconstruction of bacteriophage φ29 reveals a novel toroidal structure thought to be 30–40 bp of highly bent/twisted DNA contained in a small cavity below the capsid. Here, we extend an elastic rod model for DNA to enable simulation of the toroid as it is compacted and subsequently ejected from a small volume. We compute biologically-realistic forces required to form the toroid and predict ejection times of several nanoseconds.

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Rapporti di organizzazioni sul tema "Bacteriophages Genetics"

1

Obringer, John W. Bacteriophage T4D Gene 42 Mutants Exhibit a Defective Genetic Exclusion Phenotype. Fort Belvoir, VA: Defense Technical Information Center, febbraio 1991. http://dx.doi.org/10.21236/ada235836.

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2

Hodges, Thomas K., e David Gidoni. Regulated Expression of Yeast FLP Recombinase in Plant Cells. United States Department of Agriculture, settembre 2000. http://dx.doi.org/10.32747/2000.7574341.bard.

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Research activities in both our laboratories were directed toward development of control of the FLP/frt recombination system for plants. As described in the text of the research proposal, the US lab has been engaged in developing regulatory strategies such as tissue-specific promoters and the steroid-inducible activation of the FLP enzyme while the main research activities in Israel have been directed toward the development and testing of a copper-regulated expression of flp recombinase in tobacco (this is an example of a promoter activation by metal ions). The Israeli lab hat additionally completed experiments of previous studies regarding factors affecting the efficiency of recombinase activity using both a gain-of-function assay (excisional-activation of a gusA marker) and loss of function assay (excision of a rolC marker) in tobacco. Site-specific recombinase systems, in particular the FLP/frt and R/RS systems of yeast and the Cre/lox system of bacteriophage P1, have become an essential component of targeted genetic transformation procedures both in animal and plant organisms. To provide more flexibility in transgene excisions by the recombinase systems as well as gene targeting, and to widen possible applications, the development of controlled or regulated recombination systems is highly desirable and was therefore the subject of this research proposal. There are a few possible mechanisms to regulate expression of a recombinase system. They include: 1) control of the recombination system by having the target sites (e.g. frt) in one plant and the flp recombinase gene in another, and bringing the two together by cross fertilization. 2) regulation of promoter activities by external stimuli such as temperature, chemicals, metal ions, etc. 3) regulation of promoter activities by internal signals, i.e. cell- or tissue-specific, or developmental regulation. 4) regulation of enzyme activity by providing cofactors essential for biochemical reactions to take place such as steroid molecules in conjunction with a steroid ligand-binding protein (domains). During the course of this research our major emphasis have been focused toward studying the feasibility of hybrid seed production in Arabidopsis, using FLP/frt. Male-sterility was induced using the antisence of a pollen- and tapetum-specific gene, bcp1, isolated from Arabidopsis. The sterility inducing gene was flanked by frt sites. Upon cross pollination of flowers of male-sterile plants with pollen from FLP-containing plants, viable seeds were produced, and the progeny hybrid plants developed normally. The major achievement from this work is the first demonstration of using a site-specific recombinase to restore fertility in male-sterile plants (see attached paper, Luo et al., Plant J 2000; 23:423-430). The implication from this finding is that site-specific recombination systems can be applied in crop plants as a useful alternative method for hybrid seed production.

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