Letteratura scientifica selezionata sul tema "Allergen microarrays"

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Articoli di riviste sul tema "Allergen microarrays"

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Sokolov, Pavel, Irina Evsegneeva, Alexander Karaulov, Alyona Sukhanova, and Igor Nabiev. "Allergen Microarrays and New Physical Approaches to More Sensitive and Specific Detection of Allergen-Specific Antibodies." Biosensors 14, no. 7 (2024): 353. http://dx.doi.org/10.3390/bios14070353.

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Abstract (sommario):
The prevalence of allergic diseases has increased tremendously in recent decades, which can be attributed to growing exposure to environmental triggers, changes in dietary habits, comorbidity, and the increased use of medications. In this context, the multiplexed diagnosis of sensitization to various allergens and the monitoring of the effectiveness of treatments for allergic diseases become particularly urgent issues. The detection of allergen-specific antibodies, in particular, sIgE and sIgG, is a modern alternative to skin tests due to the safety and efficiency of this method. The use of al
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Gipson, Stephen A. Y., Jacqueline B. Nesbit, Lauren T. Swientoniewski, et al. "Purification and Epitope Mapping of Jug r 4, a Major Walnut Allergen." Allergies 5, no. 1 (2025): 8. https://doi.org/10.3390/allergies5010008.

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Abstract (sommario):
Background: Tree nut allergy affects approximately 1% of the U.S. population and the prevalence is increasing. Walnut allergy is the most commonly reported tree nut allergy in the United States. This study aimed to investigate the IgE cross-reactivity between walnut allergen Jug r 4 and peanut allergen Ara h 3 in individuals with dual walnut and peanut allergies. Methods: Jug r 4 was purified from whole walnut extract and analyzed via western blot using anti-Ara h 3 antibodies alongside serum IgE from walnut allergic patients. Sera from individuals allergic to both peanuts and walnuts were uti
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Klimek, L., D. Vetter, L. von Bernus, and C. Thorn. "Allergen-Microarrays für die molekulare Komponentendiagnostik von Typ-I-Allergien." HNO 59, no. 10 (2010): 988–93. http://dx.doi.org/10.1007/s00106-010-2224-5.

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Romantowski, Jan, Aleksandra Górska, Grażyna Moszkowska, et al. "Atopy and Multisensitizations in Specific IgE Microarrays and Their Impact on Severe Asthma." Life 12, no. 10 (2022): 1520. http://dx.doi.org/10.3390/life12101520.

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Abstract (sommario):
(1) Asthma is a chronic inflammatory airway disease. Around 3–10% of patients experience severe refractory asthma. These patients with high symptom intensity and frequent exacerbations present a challenge for allergologists. Their allergic vs. non-allergic profile might be different from the standard asthmatic group and this difference is vital in qualifying for anti-IgE biologicals. The aim of the study was to analyze multiple sensitizations in patients with severe asthma and assess their impact on the course of the disease. (2) Forty-two patients with severe asthma according to GINA were enr
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Cretich, Marina, Daniela Breda, Francesco Damin, et al. "Allergen microarrays on high-sensitivity silicon slides." Analytical and Bioanalytical Chemistry 398, no. 4 (2010): 1723–33. http://dx.doi.org/10.1007/s00216-010-4077-x.

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Jeon, Hyunjin, Joo Hyun Jung, Yoonji Kim, B.S., Youngeun Kwon, and Seon Tae Kim. "Allergen Microarrays forIn VitroDiagnostics of Allergies: Comparison with ImmunoCAP and AdvanSure." Annals of Laboratory Medicine 38, no. 4 (2018): 338–47. http://dx.doi.org/10.3343/alm.2018.38.4.338.

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Szameit, Sandra, Elisabeth Weber, and Christa Noehammer. "DNA microarrays provide new options for allergen testing." Expert Review of Molecular Diagnostics 9, no. 8 (2009): 843–50. http://dx.doi.org/10.1586/erm.09.63.

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Baumgart, Karl. "The whether, whither or wither of allergen microarrays." Pathology 56 (February 2024): S31. http://dx.doi.org/10.1016/j.pathol.2023.12.114.

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Romantowski, Jan, Aleksandra Górska, Marek Niedoszytko, et al. "A Challenge for Allergologist: Application of Allergy Diagnostic Methods in Mast Cell Disorders." International Journal of Molecular Sciences 22, no. 3 (2021): 1454. http://dx.doi.org/10.3390/ijms22031454.

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Abstract (sommario):
Primary and secondary mast cell activation syndromes (MCAS) can occur in patients with mastocytosis. During the past few years our knowledge about the pathogenesis and disease-triggering mechanisms in MCAS and mastocytosis have increased substantially. Whereas mastocytosis is characterized by an accumulation of neoplastic (clonal) mast cells (MC) in various organ systems, MCAS is defined by a massive and systemic activation of these cells. Mast cells are crucial effector cells in allergic diseases, thus their elevated number and activation can cause severe anaphylactic reactions and MCAS in pa
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Chinnasamy, Thiruppathiraja, Loes I. Segerink, Mats Nystrand, Jesper Gantelius, and Helene Andersson Svahn. "Point-of-Care Vertical Flow Allergen Microarray Assay: Proof of Concept." Clinical Chemistry 60, no. 9 (2014): 1209–16. http://dx.doi.org/10.1373/clinchem.2014.223230.

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Abstract BACKGROUND Sophisticated equipment, lengthy protocols, and skilled operators are required to perform protein microarray-based affinity assays. Consequently, novel tools are needed to bring biomarkers and biomarker panels into clinical use in different settings. Here, we describe a novel paper-based vertical flow microarray (VFM) system with a multiplexing capacity of at least 1480 microspot binding sites, colorimetric readout, high sensitivity, and assay time of <10 min before imaging and data analysis. METHOD Affinity binders were deposited on nitrocellulose membranes by conve
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Tesi sul tema "Allergen microarrays"

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Martinroche, Guillaume. "Quantification et caractérisation des maladies auto-immunes et allergiques à l'aide de méthodes d'apprentissage profond." Electronic Thesis or Diss., Bordeaux, 2024. http://www.theses.fr/2024BORD0154.

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Abstract (sommario):
Les outils d’aide au diagnostic utilisant l’intelligence artificielle (IA) aideront très prochainement les praticiens à proposer une médecine plus personnalisée et de précision pour les patients. Les maladies auto-immunes et allergiques (MAIA) sont le parfait exemple de maladies au diagnostic complexe pouvant bénéficier de ces outils. Les anticorps antinucléaires (ANA) sur les cellules épithéliales humaines (HEp-2) sont la référence pour le dépistage et le diagnostic des maladies auto-immunes (MAI). Pour une harmonisation des pratiques de laboratoires et cliniques, une lecture et une classific
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Ardizzoni, Andrea. "Allergome microarray for the detection of total repertoire of allergen-specific IgE human serum." Thesis, Imperial College London, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.427750.

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Hamed, Aljali. "Development of IgE microarray assays for classification of allergic individuals." Thesis, University of Nottingham, 2016. http://eprints.nottingham.ac.uk/37431/.

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Abstract (sommario):
Allergic reactions are pathological immune reactions against common, harmless environmental proteins, termed “allergens”. Type I hypersensitivity reactions are mediated by IgE antibodies; these reactions are the most prevalent, affecting all ages and both genders to almost the same extent. This form of allergic reaction is distributed widely affecting more than 25% of the population in the industrial world. The increased incidence of IgE-mediated allergy is thought to be a combination of environmental and genetic factors, each account for about 50%. The effects associated with this disease may
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Erkers, Julia. "Towards automatic smartphone analysis for point-of-care microarray assays." Thesis, Uppsala universitet, Institutionen för biologisk grundutbildning, 2016. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-280663.

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Poverty and long distances are two reasons why some people in the third world countries hasdifficulties seeking medical help. A solution to the long distances could be if the medical carewas more mobile and diagnostically tests could be performed on site in villages. A new pointof-care test based on a small blood shows promising results both in run time and mobility.However, the method still needs more advanced equipment for analysis of the resultingmicroarray. This study has investigated the potential to perform the analysis within asmartphone application, performing all steps from image capt
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Bosco, Anthony. "Identification of novel genes associated with allergen-driven T cell activation in human atopics." University of Western Australia. School of Paediatrics and Child Health, 2007. http://theses.library.uwa.edu.au/adt-WU2007.0023.

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Abstract (sommario):
[ Truncated abstract ] Atopic diseases such as asthma are thought to be driven to a significant extent by T helper memory cells which are programmed to respond in a harmful way to environmental allergens (e.g. house dust mite). Previous studies in humans and in animal models have established that activation of TH2 cytokine genes in T memory responses to allergens is central to the disease process. However, only a subset of atopics harbouring a TH2-memory response phenotype manifests clinical symptoms of disease. Moreover, clinical trials with TH2 antagonists in atopic patients have proven dis
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Wang, Xiaowei. "Development of a detection system towards a basophil-microarray for the diagnosis of allergies." Thesis, University of Nottingham, 2014. http://eprints.nottingham.ac.uk/14187/.

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Abstract (sommario):
Allergic responses are mainly mediated by immunoglobulin E (IgE) and mast cells or basophils expressing the high-affinity IgE receptor FcεRI. Cross-linking of FcεRI and IgE complexes with allergen induces basophil degranulation and release of inflammatory chemical mediators, leading to clinical symptoms. Common allergy diagnostic tests such as ImmunoCAP, focused on the measurement of specific IgE in patients, commonly lead to misdiagnosis. The allergen-specific IgE in patients’ sera might not always lead to FcεRI cross-linking on mast cells or basophils, resulting in no related clinical sympto
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Mallmann, Stefanie [Verfasser], and Ralf S. [Akademischer Betreuer] Müller. "Untersuchung der Testreaktionen in Allergen-Microarray und Intradermaltest von Hunden mit atopischer Dermatitis unter Berücksichtigung der allergisch bedingten Juckreizsymptomatik / Stefanie Mallmann ; Betreuer: Ralf S. Müller." München : Universitätsbibliothek der Ludwig-Maximilians-Universität, 2017. http://d-nb.info/1216827230/34.

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Pascal, i. Capdevila Mariona. "Allergenic protein and epitope recognition in food allergy: a new perspective for the molecular and clinical characterization of shellfish and lipid transfer protein allergy / Reconeixement de proteïnes i epítops al•lergènics en al•lèrgia alimentaria: una nova perspectiva per a la caracterització clínica i molecular de l’al•lèrgia al marisc i a les proteïnes de transferència de lípids." Doctoral thesis, Universitat de Barcelona, 2012. http://hdl.handle.net/10803/84070.

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Abstract (sommario):
Currently food allergy diagnostic tests are not able to predict clinical reactivity in sensitized patients (those with specific IgE against a particular allergen). Traditionally, allergy diagnostic tests used complete extracts of allergenic sources containing multiple molecules, some allergic and others not. This greatly limits the accuracy in the diagnosis and identification of possible allergic reactions to different foods by the existence of cross reactivity. Through the last decades, thanks to advances in the characterization of allergens at molecular level, the concept of Component-Based
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ARASI, STEFANIA. "Reliable mite-specific microarray for testing IgE antibodies in nasal secretions." Doctoral thesis, 2017. http://hdl.handle.net/11570/3121575.

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Methods to measure allergen-specific immunoglobulin E (sIgE) at nasal level in patients with allergic rhinitis are currently of great interest for many reasons, such as the evidence for local production of IgE in the nasal mucosa. Nasal secretions are easily collected with various non-invasive procedures and suitable for IgE measurements. Nevertheless, IgE “classic” assays, based on immunoenzymatic methods and allergen extracts, require large volumes and are inefficient in detecting the low amounts of IgE in NasSec. In this study, IgE antibodies to 15 allergen molecules of Dermatophagoides pte
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Pereira, Cátia Liliana Morais. "Characterization of allergens from several tree nuts and their role in plant food allergy." Master's thesis, 2013. http://hdl.handle.net/10451/11516.

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Tese de mestrado, Controlo da Qualidade e Toxicologia dos Alimentos, Universidade de Lisboa, Faculdade de Farmácia, 2014<br>Food allergies are a common issue in western countries. In the last decade, these diseases has increased significantly, and nowadays it is estimated that affects 2-8% of the population. Within the food allergies, plant food is the most frequent in adult population and the most part of the plant food allergens belong to protein families with defense or storage functions. Among plant food allergies there is a special interest in tree nut allergy. In the course of history,
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Libri sul tema "Allergen microarrays"

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DNA microarrays for biomedical research: Methods and protocols. Humana Press, 2009.

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Jin, Saihong. Specific IgE profiling of 52 allergens in sensitized individuals using a new protein microarray technology. 2005.

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Capitoli di libri sul tema "Allergen microarrays"

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Bacarese-Hamilton, Tito, Julian Gray, Andrea Ardizzoni, and Andrea Crisanti. "Allergen Microarrays." In Microarrays in Clinical Diagnostics. Humana Press, 2005. http://dx.doi.org/10.1385/1-59259-923-0:195.

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Kalli, Marina, Marcos J. C. Alcocer, Andrew J. Blok, and Franco H. Falcone. "Use of Humanized Fluorescent Reporter Cell Line RBL NFAT-DsRed for the Detection of Allergen-Specific IgE in Patient Sera Using Allergen Microarrays." In Basophils and Mast Cells. Springer US, 2020. http://dx.doi.org/10.1007/978-1-0716-0696-4_12.

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Lin, Jing, Ludmilla Bardina, and Wayne G. Shreffler. "Microarrayed Allergen Molecules for Diagnostics of Allergy." In Epitope Mapping Protocols. Humana Press, 2009. http://dx.doi.org/10.1007/978-1-59745-450-6_19.

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Harwanegg, Christian, Sabine Hutter, and Reinhard Hiller. "Allergen Microarrays for the Diagnosis of Specific IgE Against Components of Cow Milk and Hen Egg in a Multiplex Biochip-Based Immunoassay." In Methods in Molecular Biology. Humana Press, 2007. http://dx.doi.org/10.1007/978-1-59745-426-1_11.

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De Chan, Xin, and Chuping Mu. "Sensitisation Profiles of House Dust Mite-Allergic Subjects Using an Allergen Microarray Platform." In Proceedings of the 9th IRC Conference on Science, Engineering, and Technology. Springer Nature Singapore, 2023. http://dx.doi.org/10.1007/978-981-99-8369-8_29.

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Jambari, Nuzul N., XiaoWei Wang, and Marcos Alcocer. "Protein Microarray-Based IgE Immunoassay for Allergy Diagnosis." In Methods in Molecular Biology. Springer New York, 2017. http://dx.doi.org/10.1007/978-1-4939-6925-8_10.

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Osin, Nikolay S., and Vera G. Pomelova. "Microarray Immunophosphorescence Technology for the Detection of Infectious Pathogens." In National Institute of Allergy and Infectious Diseases, NIH. Humana Press, 2008. http://dx.doi.org/10.1007/978-1-59745-569-5_25.

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Martínez-Botas, Javier, and Belén de la Hoz. "IgE and IgG4 Epitope Mapping of Food Allergens with a Peptide Microarray Immunoassay." In Methods in Molecular Biology. Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-3037-1_18.

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Martínez-Botas, Javier, Carlos Fernández-Lozano, Aida Vaquero-Rey, and Belén de la Hoz. "IgE and IgG4 Epitope Mapping of Food Allergens with a Peptide Microarray Immunoassay." In Methods in Molecular Biology. Springer US, 2022. http://dx.doi.org/10.1007/978-1-0716-2732-7_16.

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Tortajada-Genaro, Luis Antonio. "Building Reversed-Phase Microarrays on Low-Cost Biosensing Chips: Application to Multiplexed Detection of Food Allergies." In Methods in Molecular Biology. Springer US, 2025. https://doi.org/10.1007/978-1-0716-4595-6_2.

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Atti di convegni sul tema "Allergen microarrays"

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Vikalo, Haris, and Babak Hassibi. "Limits of performance of real-time DNA microarrays." In 2009 47th Annual Allerton Conference on Communication, Control, and Computing (Allerton). IEEE, 2009. http://dx.doi.org/10.1109/allerton.2009.5394924.

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Chou, Shan-Ying, Jia-Han Wu, Shang-Ta Chou, et al. "An integrated microfluidic system for automating multiplex allergy microarrays." In 2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems (NEMS). IEEE, 2017. http://dx.doi.org/10.1109/nems.2017.8017059.

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Ji, Xia, Weizhong Zhang, Shao-dan Jia, et al. "A Microarray Gene Expression Profile in Peripheral Blood Mononuclear Cells for Allergic Asthma in Chinese." In Biomedical Engineering. ACTAPRESS, 2012. http://dx.doi.org/10.2316/p.2012.764-007.

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