Bibliografie tematiche / 823/.8

Letteratura scientifica selezionata sul tema "823/.8"

Autore: Grafiati
Pubblicato: 25 luglio 2024
Ultima modifica: 31 luglio 2024

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Articoli di riviste sul tema "823/.8"

1

Jeon, Jae Yeol, Ryo Matsumoto e Hiroshi Utsunomiya. "Feasibility Study on Die Quenching of AA2024 Aluminum Alloy Billet Using Servo Press". Advanced Materials Research 922 (maggio 2014): 286–91. http://dx.doi.org/10.4028/www.scientific.net/amr.922.286.

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Die quenching of an AA2024 aluminum alloy billet was carried out using a servo press with ram motion control of WC-20mass%Co dies directly after solution heat treatment (SHT). The cylindrical billets were heated in an electric furnace and transferred to the press, then subjected to uniaxial compression with a reduction in height (Δh/h0) of 2%, 5% or 10% after SHT at 773 K or 823 K. The die quenching was successfully carried out without the precipitation hardening only on the billet after SHT at 823 K by sandwiching for 8 s. The subsequent artificial aging behavior at 463 K in an oil bath was investigated. It is found that the precipitation kinetics is accelerated in the die-quenched billets after SHT at 823 K. The peak hardness of the billet processed with 5% after SHT at 823 K is as high as that of the water-quenched billet, HV 149.0.
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2

Yang, Ruichuang, Jianxia Wen, Tao Yang, Chunmei Dai e Yanling Zhao. "Evodiamine Inhibits Gastric Cancer Cell Proliferation via PTEN-Mediated EGF/PI3K Signaling Pathway". Evidence-Based Complementary and Alternative Medicine 2021 (16 novembre 2021): 1–15. http://dx.doi.org/10.1155/2021/5570831.

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Aims. In this study, the pharmacological effects and potential molecular mechanisms of evodiamine in treating gastric cancer (GC) were investigated. Methods. GC cells lines of AGS and BGC-823 were treated with evodiamine at various concentrations for different times (24, 48, and 72 h). Inhibition of the proliferation of AGS and BGC-823 cells was assessed using a CCK-8 assay. The morphology of gastric cancer cells was detected by high-content screening (HCS). The apoptosis-inducing effect of evodiamine on AGS and BGC-823 cells was detected by flow cytometric analysis. Cell migration and invasion were detected by Transwell assay. The relative mRNA and protein expression levels of PTEN-mediated EGF/PI3K signaling pathways were investigated via RT-qPCR or western blotting, respectively. Results. Evodiamine substantially inhibited AGS and BGC-823 cells proliferation in a dose- and time-dependent manner. Flow cytometric analysis revealed that evodiamine could induce apoptosis of AGS and BGC-823 cells in a dose-dependent manner. In addition, evodiamine inhibited AGS and BGC-823 cell migration and invasion. Mechanistically, the results demonstrated that evodiamine promoted the relative mRNA and protein expression of PTEN and decreased expression of EGF, EGFR, PI3K, AKT, p-AKT, and mTOR. Most importantly, evodiamine could effectively increase the mRNA and protein expression of PTEN and decrease the protein expression of EGF/PI3K pathway, indicating that evodiamine downregulated EGF/PI3K through the activation of PTEN pathway. Conclusion. Evodiamine inhibited the directional migration and invasion of GC cells by inhibiting PTEN-mediated EGF/PI3K signaling pathway. These findings revealed that evodiamine might serve as a potential candidate for the treatment or prevention of GC.
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3

Li, Shaoying, Xiaojun Xi, Yiwa Luo, Mingtao Mao, Xiao Shi, Jing Guo e Hanjie Guo. "Carbide Precipitation during Tempering and Its Effect on the Wear Loss of a High-Carbon 8 Mass% Cr Tool Steel". Materials 11, n. 12 (7 dicembre 2018): 2491. http://dx.doi.org/10.3390/ma11122491.

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In this paper, the precipitation of carbide and wear loss of high-carbon 8 mass% Cr tool steel at two tempering conditions (i.e., 773–803 K and 823–853 K) were studied by INCA Steel, EPMA-1720H, XRD, and ML-10 tester. The results show that the particles of test steels include the carbides (Cr7C3 and Cr23C6) and carbides nucleated on Al2O3. When carbides are of the same size, the number of carbides in test steel at a tempering temperature of 773–803 K is greater than that at a tempering temperature of 823–853 K, especially when the size of carbides is less than 5 μm. Compared with the test steel tempered at 823–853 K, the distance between adjacent actual particles reduced by 80.6 μm and the maximum amount of reduction was 9.4% for single wear loss at the tempering temperature of 773–803 K. It can be concluded from thermodynamics results that Al2O3 inclusions began to precipitate in liquid, and the precipitation of carbides was at the solid–liquid region. Al2O3 can be used as the nucleation interface of carbide, thus promoting the formation of carbides. During the cooling of molten steel, a lower temperature can increase the difference of actual solubility product bigger than equilibrium solubility product, thus promoting the carbide formation.
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4

Chen, Zhou, Dan Hong, Siting Li e Yingmin Jia. "Novel Property Cytotoxicity and Mechanism of Food Preservative Brevilaterins against Human Gastric Cancer Cells". Foods 12, n. 8 (21 aprile 2023): 1732. http://dx.doi.org/10.3390/foods12081732.

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Brevilaterins, antimicrobial peptides produced by Brevibacillus laterosporus, are regarded as excellent food preservatives and are popular as antimicrobial applications. Recent research has uncovered their potent cytotoxic effects against diverse cancer cells, thereby underscoring the pressing need for more extensive and intensive investigations into this use. In this study, we explored their novel function in inducing cytotoxicity to cancer cells and systematically investigated the mechanism of action of Brevilaterin B/C (BB/BC) in vivo. Proliferation, membrane permeability, and apoptotic rate were evaluated using CCK-8 assay, LDH assay, and Annexin V-FITC/PI kits. ROS levels and mitochondrial membrane potential were detected using the fluorescent probe DCFH-DA and JC-1. Our results demonstrated that both BB and BC at concentrations of 4–6 µg/mL significantly inhibited the proliferation and migration of gastric cancer cells BGC-823. Treatment with 4 µg/mL of BB/BC rapidly increased LDH levels in the supernatant of BGC-823 cells, leading to further investigation of the mechanism of apoptosis. We found that the apoptotic rate of BGC-823 cells significantly increased upon treatment with BB/BC, demonstrating their potent induction of apoptosis. BB/BC-induced ROS production in BGC-823 cells impaired their growth and induced apoptosis, indicating a close association between apoptosis and ROS elevation. Additionally, JC-1 aggregates rapidly accumulated after treatment with 4 µg/mL of BB/BC, suggesting changes in mitochondrial membrane potential and early apoptosis. Taken together, our findings revealed that BB and BC exhibit significant anticancer effects against gastric cancer cells, highlighting the promising potential of Brevilaterins as anticancer agents.
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Xu, Jian, Guang-Zhi Zeng, Ke-Li Chen, Yi-Mei Liu, Zhang-Hua Sun, Ning-Hua Tan e Yu-Mei Zhang. "A New Lignan Glycoside from Chamaecyparis obtusa var. breviramea f. crippsii". Natural Product Communications 9, n. 2 (febbraio 2014): 1934578X1400900. http://dx.doi.org/10.1177/1934578x1400900220.

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A new lignan glycoside, (-)-(8 S, 8′ R)-thujastandin-4- O-β-D-glucopyranoside (1), together with fourteen known lignanoids (2–15) and one coumarin (16) were isolated from the branches and leaves of Chamaecyparis obtusa var. breviramea f. crippsii. Their structures were elucidated by extensive spectroscopic and spectrometric analysis. Compound 16 exhibited cytotoxicity against A549, BGC-823 and Hela cell lines with IC50 values of 25.9, 20.9 and 18.5 μM, respectively.
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Liu, Huan, Yong-Ming Yan, Li Liao, Shao-Xiang Wang, Yi Zhang e Yong-Xian Cheng. "Cicadamides A and B, N-Acetyldopamine Dimers From the Insect Periostracum cicadae". Natural Product Communications 14, n. 6 (giugno 2019): 1934578X1985001. http://dx.doi.org/10.1177/1934578x19850019.

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Two new N-acetyldopamine dimers, cicadamides A (1) and B (2), and 8 known compounds were isolated from the medicinal insect Periostracum cicadae. Their structures including absolute configurations were assigned by spectroscopic and computational methods. Biological activities of compounds 1, (+)-2, and (–)-2 toward human cancer cells BGC-823, HepG2, and A549 were evaluated.
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7

Yakufu, Mirensha, Renaguli Hailiwu, Yuanyuan Cong, Ayijiang Habaike, Xiaomei Wang e Palida Abulizi. "Antitumor activity of flavonoids from Alpinia officinarum hance on gastric cancer". European Journal of Inflammation 19 (gennaio 2021): 205873922110511. http://dx.doi.org/10.1177/20587392211051119.

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Introduction: To investigate antitumor activity and mechanism of flavonoids from Alpinia officinarum Hance against gastric cancer. Methods: Transplanted mouse fore-stomach carcinoma (MFC) tumor mice were divided into six groups: control group, model group, low dose (20 mg/kg), middle dose (40 mg/kg), and high dose (80 mg/kg) groups of TFAO and 5-Fu group. Mice were treated with TFAO or 5-Fu for 14 days, except those of control and model group. Tumor inhibitory rate, spleen, and thymus index were calculated. Contents of proliferating cell nuclear antigen, MMP-9, vascular endothelial growth factor, IL-1β, IL-6, and IL-17 in serum were detected. Effect of galangin on BGC-823 cell growth was detected. Cell apoptosis and cell cycle distribution were measured. Enzyme activity of Caspase-3, Caspase-8, and Caspase-9 was detected. Western blot was used to detect STAT3, Bcl-2, Bax, Caspase-3, Caspase-8, Caspase-9, CyclinB1, and CyclinD1 protein expression in BGC-823 cell. Results: Compared with model group, tumor weight of mice decreased significantly ( p < .01) in 5-Fu group, low dose, middle dose, and high dose group of TFAO; thymus index of mice decreased significantly ( p < .05) in 5-Fu group; and spleen index decreased significantly ( p < .05) in low dose and middle dose groups of TFAO. Compared with model group, levels of PCNA, MMP-9, IL-1β, and IL-6 in serum of mice decreased obviously ( p < .01) in all administration groups; levels of VEGF in serum of mice decreased obviously ( p < .01) in low dose and high dose group of TFAO and 5-Fu group; and levels of IL-17 in serum of mice decreased significantly ( p < .01) in low-dose and middle-dose groups of TFAO and 5-Fu group. Galangin could inhibit BGC-823 cell growth; accelerate apoptosis; block cell cycle; increase cell Caspase-3, Caspase-8, and Caspase-9 enzyme activity; upregulate expression of Caspase-3, Caspase-8, Caspase-9, and Bax; and downregulate expression of STAT3, CyclinB1, CyclinD1, and Bcl-2 protein. Conclusion: Flavonoids from A. officinarum showed antitumor activity in gastric cancer. Mechanisms may be associated with inhibition of tumor angiogenesis, tumor cell proliferation, and cancer-associated inflammation.
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8

Fernández Ochoa, Carmen. "Reseña de: Hidalgo Prieto, Rafael (Coord.): Las Villas Romanas de la Bética". Espacio Tiempo y Forma. Serie I, Prehistoria y Arqueología, n. 11 (11 dicembre 2018): 149. http://dx.doi.org/10.5944/etfi.11.2018.23119.

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Reseña de Hidalgo Prieto, Rafael (Coord.): Las Villas Romanas de la Bética, vol. I y II, Ed. Universidad de Granada (ISBN: 978-84-338-6107-8), Universidad de Córdoba (ISBN: 978-84-9927-325-9), Universidad Pablo de Olavide (ISBN: 978-84-617-7532-3), Universidad de Sevilla (ISBN: 978-84-472-1861-5), Universidad de Málaga (ISBN: 978-84-9747-8298), Sevilla, 2016, 823 pgs.
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Zhou, Qiang, Jing Hou, Huamin Li, Li Cui, Han Jia, Bing Gong e Lan He. "Synthesis and Antineoplastic Activity of Quinoline Derivatives". Australian Journal of Chemistry 61, n. 7 (2008): 531. http://dx.doi.org/10.1071/ch07419.

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Designed as a new series of molecules that contain the quinoline substructure, several 11H-indolo[3.2-c]quinoline derivatives were synthesized and subjected to biological evaluation. Several compounds were found to exhibit cytotoxic activity against the growth of colon (HTC-8), liver (BEL-7402), gastric (BCG-823), pulmonary gland (A549), and ovary (A2780) cancer cell lines. The structure–activity relationship of these compounds is discussed.
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Karpova, Darja, Michael P. Rettig, Linda Eissenberg, Julie Ritchey, Matthew Holt, Richard Heier, Stacy Arnett et al. "Combined Targeting of CXCR2 and VLA4 Results in Rapid and Synergistic Mobilization of Hematopoietic Stem and Progenitor Cells in Mice". Blood 128, n. 22 (2 dicembre 2016): 659. http://dx.doi.org/10.1182/blood.v128.22.659.659.

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Abstract Introduction : Since the first description of hematopoietic stem and progenitor cell (HSPC) mobilization over forty years ago, it has become the standard of care for both autologous and allogeneic transplantation. A five-day course of G-CSF represents the most commonly used mobilization regimen today. The CXCR4 inhibitor, plerixafor, is a more rapid but weak mobilizer when used as a single agent, thus emphasizing the need for faster acting agents with more predictable mobilization responses and fewer side effects. Methods : Given the critical role of VLA4/VCAM1 signaling for migration and retention of HSPC, we were seeking to identify small molecule antagonists of VLA4 with improved potency and bioavailability. Relative to previously described comparators Bio5192 (▢4▢1-specific) and firategrast (▢4▢1 and ▢4▢7 dual-specific), our lead candidate, CWHM-823, exhibited increased aqueous solubility and ~10-100 fold better activity in blocking VLA4 and mobilizing HSPC in mice. CWHM-823 pharmacokinetics and mobilization were assessed in BALB/c and DBA/2 mice at different doses (3 to 15 mg/kg) and time points (15 to 240 min) when administered alone or in combination with the truncated isoform of the CXCR2 agonist Gro-beta (tGro-β, 2.5 mg/kg, generously provided by GlaxoSmithKline). HSPC mobilization was monitored using flow cytometry and clonogenic in vitro assays. "True" stem cells were measured in a serial competitive transplantation assay. The combination of tGro-β and VLA4 antagonist was further tested in diabetic mice in comparison to G-CSF (9 x 100μg/kg, q12h). RNA profiling of flow-sorted HSPC was performed via microarray analysis. Results : The combination of tGro-β with each VLA4 antagonist resulted in a dramatic synergistic increase in circulating HSPC numbers when compared to steady state (50-70-fold) or treatment with single agents (3-10 fold) including tGroβ. Mobilization with tGro-β + CWHM-823 was rapid, peaking at 15-30 minutes after injection. In a model of streptozotocin-induced diabetes, the mobilopathy (reduction in stem cell mobilization compared to wild type mice) was considerably less pronounced with the combination tGro-β + CWHM-823 (~1.5-fold lower CFU mobilization in diabetic mice) versus the 5-day course of G-CSF (~3-fold reduction). Despite the superior progenitor cell mobilization achieved with G-CSF (~2-fold more CFU and LSK/ml), the concentration of serially repopulating units (RU) was equally high in the tGro-β + CWHM-823 and G-CSF mobilized grafts suggesting a higher HSC frequency (1 RU out of 200 vs. 1 RU out of 400 LSK/CFU) in the tGro-β + CWHM-823 mobilized grafts (Figure 1). RNA profiling demonstrated close similarity between the expression profile of tGro-β + CWHM-823 mobilized, BM resident, and G-CSF mobilized LSK, with less than 0.5% of genes found to be significantly up- or downregulated. CXCR2 chemokine receptor stimulation was critical for the observed synergistic response, as pretreatment ("priming") or simultaneous treatment with tGro-β resulted in subsequent enhanced mobilization using VLA4 inhibitors, whereas reversed administration (VLA4 antagonist followed by tGro-β) had no effect on potency of either agent. Lack of surface CXCR2 expression on HSPC suggested that a rapidly acting effector molecule released from tGro-β-stimulated mature myeloid cells may subsequently influence VLA4-mediated HSPC adhesion/retention. Consistent with this theory, we observed increased protease MMP-9 in plasma within minutes after treatment with tGro-β + CWHM-823. Conclusions: We describe a novel strategy for rapid, reliable, and potent mobilization of HSPC in mice using a combination of VLA4 blockade (via novel and potent ▢4▢1 inhibitors) and CXCR2 activation (via tGro-β). The combination of tGro-β + VLA4 inhibitors or tGro-β followed by VLA4 inhibitors results in synergistic and rapid HSPC mobilization with quantity and quality of repopulating units similar to optimal mobilization with G-CSF. These data suggest further development of tGro-β + VLA4 inhibitor combinations for clinical testing is warranted. Figure 1. Mobilization of repopulating units (RU) (n=8-10 recipients per group, mean±SEM) Figure 1. Mobilization of repopulating units (RU) (n=8-10 recipients per group, mean±SEM) Disclosures No relevant conflicts of interest to declare.
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Tesi sul tema "823/.8"

1

Carlsson, Peter. "Nuclear receptors studied by molecular dynamics computer simulations /". Stockholm, 2004. http://diss.kib.ki.se/2004/91-7349-823-8.

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2

Faizer, Rizwi Shuhadha 1946. "Ibn Isḥâq and al-Wâqidî revisited : a case study of Muḥammad and the Jews in biographical literature". Thesis, McGill University, 1995. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=28738.

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Si ra-maghazi, which tells of the life of the Prophet and the early Islamic community, is not a historical genre. A literary mode which has its origins in an oral transmission, it is essentially hagiographic in spirit. The literature carries some unique characteristics. Constituted of numerous individual traditions juxtaposed one next to the other, it is--other than for those key events that have become mythologized--essentially dependent on the compiler and his purpose for its layout.
This dissertation explores the genre through a comparative case study of Muhammad and the Jews as narrated in the Kitab si rat rasul Allah of lbn Ishaq and the Kitab al-maghazi of al-Waqidi. Appreciating the interpretation of the individual compiler concerned, it compares, in terms of method, structure, sources, chronology, and style, their different approaches to the subject of the early establishment of Islam. The differences reinforce the argument for appreciating si ra-maghazi as literary rather than a historical genre. More importantly, they bring into focus the tendentious nature of si ra-maghazi to understand why neither one of these texts may be used to substantiate the information in the other.
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3

Modigh, Johan. "5,56 x 45 mm FMJ i Afghanistan : amerikanska och brittiska stridserfarenheter av kaliberns effekt". Thesis, Swedish National Defence College, Swedish National Defence College, 2010. http://urn.kb.se/resolve?urn=urn:nbn:se:fhs:diva-823.

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Uppsatsens huvudsyfte är att undersöka om amerikanska och brittiska stridserfarenheter av kalibern 5,56 i Afghanistan kan bidra till att utveckla den svenska Försvarsmaktens taktiska förmåga. Anledningen till varför jag valde att utgå från dessa länder är därför att de har fler öppna källor samt har mer erfarenhet än många andra. Studien genomfördes genom en kvalitativ textanalys av bland annat reglementen, rapporter och artiklar. Uppsatsen visar att Försvarsmaktens kaliber till sin automatkarbin, 5,56, var potent för stridstaktikanpassad för invasionsförsvar. Detta delvis på grund av kaliberns egna logistiska fördelar men även därför att de prioriterade vapensystemen för infanteriförbanden, lätta pansarvärnsvapnen, innebar relativt korta stridsavstånd. Studien visar därefter att idag, för amerikanska och brittiska styrkor i dagens konflikt i Afghanistan, är 5,56 otillräcklig. Stridsavstånden är generellt för långa för att kalibern skall avge sin optimala verkan i målet. Studien belyser vad dessa nationer vidtagit för åtgärder, samt vilka åtgärder som planeras eller finns som förslag för att förbättra sin stridstaktiska förmåga. Den mest optimala förbättringen anser många är att byta ut kalibern 5,56 mot en grövre mellankaliber i spannet 5,56-7,62. Förslag som förändrad skjututbildning, optikutveckling och nya vapeninköp nämns också. I diskussionen diskuteras om eller hur den svenska Försvarsmakten kan dra nytta av de andra nationernas erfarenheter. Resonemanget ger det generella slutsatsen att Försvarsmakten kan utveckla sin stridstaktiska förmåga genom att studera andra nationer. Ett snabbt konkret exempel vore att implementera skarpskyttevapnet ak4 med förstorande optiskt hjälpmedel i svenska Försvarsmaktens skytteförband.


The main purpose with this study is to investigate whether U.S. and British combat experience of the caliber 5.56 in Afghanistan is helping to develop the Armed Forces' tactical ability. The reason why I chose to rely on these countries is because they have more unclassified sources and has more combat experience than many others. This study was conducted through a qualitative text analysis of for example regulations, reports and articles. This essay shows that the Swedish Armed Forces standard caliber that is being used in today’s assault rifle (ak5c), were potent in battle tactics designed for the defence of Sweden. This is partly becauseof 5,56 own logistical advantages and also because light anti-tank weapons had priority over small arms weapons. This resulted in relatively short shooting distances. The study shows thereafter that the U.S. and British troops in today's conflict in Afghanistan, thinks that 5.56 is insufficient. Combat distances are generally too long for the caliber to deliver its optimum effect in the target. The study highlights what kind of actions these nations have taken, and what measures are planned to improve its combat tactical ability. The study reveals that the most optimal improvement, are to replace the 5.56 with a bigger medium sized caliber. Proposals to change the marksmanship training, optics development and to purchase new weapons are also mentioned. In the end of this essay there is a discussion on, or how the Swedish Armed Forces can implement U.S and British experiences. The reasoning in the final chapter gives the general answer that the Swedish Armed Forces indeed can develop their tactical combat skills by studying other nations. An example would be to implement the older ak4 with magnifying optics that could improve the longer range capabilities of the infantry units.

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Libri sul tema "823/.8"

1

Heather, Glen, a cura di. Jane Eyre. New York: St. Martin's Press, 1997.

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2

Gustav, Klaus H., a cura di. The Rise of socialist fiction 1880-1914. Brighton: Harvester, 1987.

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3

Alexander, Thorsten. Die strafrechtliche Verantwortlichkeit für die Wahrung der Verkehrssicherungspflichten in Unternehmen. Herbolzheim: Centaurus Verlag & Media, 2005. http://dx.doi.org/10.1007/978-3-86226-823-8.

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4

1936-, Kramer Dale, e Marck Nancy, a cura di. Critical essays on Thomas Hardy: The novels. Boston, Mass: G.K. Hall, 1990.

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5

Oliphant, Margaret. Selected short stories of the supernatural. Edinburgh: Scottish Academic Press, 1985.

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6

Eliot, George. George Eliot's Daniel Deronda notebooks. New York: Cambridge University Press, 1996.

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7

1946-, Mallett Phillip, a cura di. Palgrave advances in Thomas Hardy studies. New York: Palgrave Macmillan, 2004.

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8

Cleghorn, Gaskell Elizabeth. The life of Charlotte Brontë. New York: Oxford University Press, 1996.

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9

Jamieson, Ridenhour, a cura di. Carmilla. Kansas City, Mo: Valancourt Books, 2009.

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10

Cleghorn, Gaskell Elizabeth. The life of Charlotte Brontë. London: Penguin Books, 1997.

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Capitoli di libri sul tema "823/.8"

1

Staake, Marco. "§ 8 Die Haftungnach § 823 I BGB". In Gesetzliche Schuldverhältnisse, 169–259. Berlin, Heidelberg: Springer Berlin Heidelberg, 2013. http://dx.doi.org/10.1007/978-3-642-30094-3_8.

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2

Fazeli, P. K. "8. Bone metabolism in anorexia nervosa". In Handbook of nutrition and diet in therapy of bone diseases, 191–208. The Netherlands: Wageningen Academic Publishers, 2016. http://dx.doi.org/10.3920/978-90-8686-823-0_8.

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3

Robinett, Rush D., e David G. Wilson. "Case Study #8: Wind Turbine Control Design". In Understanding Complex Systems, 273–79. London: Springer London, 2011. http://dx.doi.org/10.1007/978-0-85729-823-2_13.

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4

"Revised Session 823 Thur. 8 Apr. 1647". In The Minutes and Papers of the Westminster Assembly 1643–1652, Vol. 4: Minutes, Sessions 604–1163 (1646–1652), a cura di Chad Van Dixhoorn e David F. Wright, 494–95. Oxford University Press, 2012. http://dx.doi.org/10.1093/oseo/instance.00029505.

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5

Isaacs, Neil S. "Preface". In High Pressure Food Science, Bioscience and Chemistry, v. Elsevier, 1998. http://dx.doi.org/10.1016/b978-1-85573-823-2/50003-8.

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6

Grunst, Christian. "§ 8 Die deliktische Außenhaftung des Geschäftsleiters nach § 823 Abs. 2 BGB bei unmittelbarer Verletzung von Schutzgesetzen". In Der Geschäftsleiter im Deliktsrecht, 202–64. Verlag C.H.BECK oHG, 2016. http://dx.doi.org/10.5771/9783845283418-202.

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Atti di convegni sul tema "823/.8"

1

Pompermaier, Carolina, Cassio Fernando Paganini, Willian Ely Pin, Mateus Xavier Schenato e Tales Antunes Franzini. "TUBERCULOUS LYMPHADENITIS: A CASE REPORT". In XXIV Congresso Brasileiro de Mastologia. Mastology, 2022. http://dx.doi.org/10.29289/259453942022v32s1079.

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Tuberculous lymphadenitis is the infection of lymph nodes by Mycobacterium tuberculosis. In the USA, about 8.5% of the cases of tuberculosis (TB) were characterized by lymphadenitis. The peak occurs between 30 and 40 years of age, primarily in women. Extrapulmonary TB is usually diagnosed in immunocompromised patients. The diagnosis is given by positivity in the AFB (Alcohol-Acid Resistant Bacillus) in Ziehl-Neelsen staining by sample collected by fine-needle puncture or lymph node excision. Cyto and histological analysis demonstrate epithelial cells, caseous necrosis, and necrotic cells. Such findings, added to the presence of langerhan’s giant cells, favor the diagnosis of TB even in AFB and/or negative cultures. Mantoux test is usually positive. Culture is the definitive diagnosis. Surgical excision should be reserved for diagnostic in HIV-seronegative patients. The picture involves progressive and painless growth of the lymph node chain, which may reach 8–10 cm. One-sidedness occurs in most cases. Peripheral lymphadenopathy is common among breast pathologies. The case is unusual due to the suspicion of axillary lymphadenopathy being of neoplastic origin from compatible histopathological and immunohistochemical analysis of a core biopsy. However, after the excision of lymph node clusters, histopathology showed the absence of tumor and metastatic cells. The analysis of slides with palisaded epithelioid granulomas and caseous necrosis, however, is consistent with TB lymphadenopathy. However, some points made such a verdict difficult such as negative fungal and alcohol-acid-resistant bacilli (AFB) research, as well as the presence of lymphadenopathy in the contra lateral armpit and inguinal chains, the absence of cervical lymph node enlargement and any other suggestive symptoms of associated extra-pulmonary tuberculosis. The other possibilities include non-TB mycobacteria, Bartonella sp, fungi (Histoplasma) and parasites (Toxoplasma gondii), lymphomas, sarcomas, metastatic carcinomas, sarcoidosis, cat-scratch disease, and congenital lymphatic malformations. Treatment should be performed after the confirmation of diagnosis or when susceptibility to antimicrobials is suspected (empirical treatment). In the first 2 months of the treatment, Isoniazid, Rifampicin, Pyrazinamide, and Ethambutol were used; followed by 4 months of Isoniazid and Rifampicin. The guidelines recommend surgical excision only in unusual situations, such as therapeutic failure. Ulceration, fistulas, and abscesses are complications. A 26-year-old female, nursing mother, breastfeeding only through the left breast due to a history of clefts in the right breast and with a family history of breast cancer, was referred to the breast service due to the appearance of painless nodules in her right armpit with progressive growth. Previously, she had been treated with Amoxicillin and Azithromycin, with no change in her condition. On physical examination, a lymph node aggregate was found in the right axilla. She underwent ultrasound and mammography examinations, which showed lymph nodes, measuring 2.9×1.3, 2×1.4, and 1.3×0.8 cm in the right armpit, compatible with BI-RADS IVc classification. It was decided to suppress lactation with Cabergoline and proceed with core biopsy, which showed fibrofatty tissue with chronic inflammation and epithelioid granuloma in the anatomopathological examination, and immunohistochemistry showed the markers CKM (AE1/AE3/PCK26), GATA-3 (L50-823), and Mamoglobin A (304-1A5) all negative, compatible with metastasis of primary breast cancer. After discussion, it was decided to proceed with the removal of the fused lymph nodes at level I of the right axilla. The histopathological diagnosis showed epithelioid and palisade granulomas with caseous necrosis in the lymph nodes, with negative BAAR research. Also, laboratory examinations for syphilis, HIV, HCV, and HBV were all negative and a clean chest x-ray. This patient will start treatment for TB.
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Pannekok, H., A. J. Van Zonneveid, C. J. M. de vries, M. E. MacDonald, H. Veerman e F. Blasi. "FUNCTIONAL PROPERTIES OF DELETION-MUTANTS OF TISSUE-TYPE PLASMINOGEN ACTIVATOR". In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643724.

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Over the past twenty-five years, genetic methods have generated a wealth of information on the regulation and the structure-function relationship of bacterial genes.These methods are based on the introduction of random mutations in a gene to alter its function. Subsequently, genetic techniques cure applied to localize the mutation, while the nature of the impairedfunction could be determined using biochemical methods. Classic examples of this approach is now considered to be the elucidation of the structure and function of genes, constituting the Escherichia coli lactose (lac) and tryptophan (trp) operons,and the detailed establishment of the structure and function of the repressor (lacl) of the lac operon. Recombinant DNA techniques and the development of appropriate expression systems have provided the means both to study structure and functionof eukaryotic (glyco-) proteins and to create defined mutations with a predestinedposition. The rationale for the construction of mutant genes should preferentiallyrely on detailed knowledge of the three-dimensional structure of the gene product.Elegant examples are the application of in vitro mutagenesis techniques to substitute amino-acid residues near the catalytic centre of subtilisin, a serine proteasefrom Bacillus species and to substituteanamino acid in the reactive site (i.e. Pi residue; methionine) of α-antitrypsin, a serine protease inhibitor. Such substitutions have resulted into mutant proteins which are less susceptible to oxidation and, in some cases, into mutant proteins with a higher specific activity than the wild-type protein.If no data are available on the ternary structure of a protein, other strategies have to be developed to construct intelligent mutants to study the relation between the structure and the function of a eukaryotic protein. At least for a number of gene families, the gene structure is thought to be created by "exon shuffling", an evolutionary recombinational process to insert an exon or a set of exons which specify an additional structural and/or functional domain into a pre-existing gene. Both the structure of the tissue-type plasminogen activator protein(t-PA) and the t-PA gene suggest that this gene has evolved as a result of exon shuffling. As put forward by Gilbert (Science 228 (1985) 823), the "acid test"to prove the validity of the exon shuffling theory is either to delete, insert or to substitute exon(s) (i.e. in the corresponding cDNA) and toassay the properties of the mutant proteins to demonstrate that an exon or a set of adjacent exons encode (s) an autonomousfunction. Indeed, by the construction of specific deletions in full-length t-PA cDNA and expression of mutant proteins intissue-culture cells, we have shown by this approach that exon 2 of thet-PA gene encodes the function required forsecretion, exon 4 encodes the "finger" domain involved in fibrin binding(presumably on undegraded fibrin) and the set of exons 8 and 9 specifies kringle 2, containing a lysine-binding sit(LBS) which interacts with carboxy-terminal lysines, generated in fibrin after plasmic digestion. Exons 10 through 14 encode the carboxy-ter-minal light chain of t-PA and harbor the catalytic centre of the molecule and represents the predominant "target site" for the fast-acting endothelial plasminogen activator inhibitor (PAI-1).As a follow-up of this genetic approach to construct deletion mutants of t-PA, we also created substitution mutants of t-PA. Different mutants were constructed to substitute cDNA encoding thelight chain of t-PA by cDNA encoding the B-chain of urokinase (u-PA), in order to demonstrate that autonomous structural and functional domains of eitherone of the separate molecules are able toexert their intrinsic properties in a different context (C.J.M. de Vries et al., this volume). The possibilities and the limitations of this approach to study the structure and the function of t-PA and of other components of the fibrinolytic process will be outlined.
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