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Articles de revues sur le sujet « Zinc-based activator »

Auteur : Grafiati

Publié le 9 mars 2023

Mis à jour le 31 juillet 2025

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1

BRATSLAVETS, Bogdan. "RESTORATION OF PARTS OF AGRICULTURAL MACHINES WITH ZINC-BASED GALVANIC COATINGS." Herald of Khmelnytskyi National University. Technical sciences 309, no. 3 (2022): 82–84. http://dx.doi.org/10.31891/2307-5732-2022-309-3-82-84.

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Galvanizing is most often used to protect against corrosion. In repair production zinc coverings are used for protection against corrosion of fastening details and restoration of landing surfaces of lightly loaded details. Electroplating is the process of applying metal to the surface of parts by crystallizing it from an aqueous solution of the corresponding salts (electrolyte) when a direct current passes through them. An electrical circuit is closed by an electrolyte between two conductors called electrodes. The electrode to which the conductor of the external circuit with a plus is attached

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Mostoni, Milana, Credico, D’Arienzo, and Scotti. "Zinc-Based Curing Activators: New Trends for Reducing Zinc Content in Rubber Vulcanization Process." Catalysts 9, no. 8 (2019): 664. http://dx.doi.org/10.3390/catal9080664.

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The efficiency of sulfur vulcanization reaction in rubber industry is generally improved thanks to the combined use of accelerators (as sulphenamides), activators (inorganic oxides), and co-activators (fatty acids). The interaction among these species is responsible for the formation of intermediate metal complexes, which are able to increase the reactivity of sulfur towards the polymer and to promote the chemical cross-links between the rubber chains. The high number of species and reactions that are involved contemporarily in the process hinders the complete understanding of its mechanism de

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Heideman, Geert, Jacques W. M. Noordermeer, Rabin N. Datta, and Ben van Baarle. "Zinc Loaded Clay as Activator in Sulfur Vulcanization: A New Route for Zinc Oxide Reduction in Rubber Compounds." Rubber Chemistry and Technology 77, no. 2 (2004): 336–55. http://dx.doi.org/10.5254/1.3547827.

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Abstract Concern about the release of eco-toxic zinc species from rubbers into the environment leads to an increasing interest in potential substitutes. Although alternative metal oxides and zinc compounds as activators for sulfur vulcanization have been studied thoroughly, at present no viable alternative has been found to eliminate ZnO completely from rubber compounds, without significantly jeopardizing processing as well as performance characteristics. In this paper, the application of a new activator for sulfur vulcanization will be discussed. This activator is developed based on the assum

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Javadi, Seyyed Mohammad. "Applications of ZnO and MgO Nanoparticles in Reducing Zinc Pollution Level in Rubber Manufacturing Processes: A Review." Current Biochemical Engineering 6, no. 2 (2020): 103–7. http://dx.doi.org/10.2174/2212711906666200224105931.

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Background: Rubber vulcanization is a consolidated chemical process to enhance the mechanical properties of the polymeric material by sulfur crosslinking of the polymer chains, such as rubber. Vulcanization Activators are important rubber processing additives that activate sulfur cure and improve the efficiency of sulfur-based cure systems. The most common activator is zinc fatty acid ester that is often formed in-situ by the reaction of fatty acid with zinc oxide. Although zinc is one of the less harmful heavy metals, according to European Council Directive 2004/73/EC, the reduction of zinc l

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Guo, Lijie, Lei Zhang, Yue Zhao, et al. "Study On Mechanical Properties Of Cemented Paste Backfill Using Fly-Ash-Based Cementitious Materials." Journal of Asian Concrete Federation 10, no. 1 (2024): 1–7. http://dx.doi.org/10.18702/acf.2023.10.1.1.

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In Mongolia, the Ulan lead-zinc mine is now struggling with high backfill operating costs and poor Cemented Paste Backfill (CPB) mechanical performance after curing. To solve this problem, BGRIMM Technology Group has investigated an activator that could mix with fly ash from Choibalsan Power Plant in Mongolia as a supplementary cementitious material for backfill. In this study, the mechanical performance of CPB samples prepared with various fly ash content and activator types were studied to provide a basis for the industrial application of fly ash-based supplementary cementitious materials. T

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Setianto, Wahyu Bahari, Nasruddin Nasruddin, Astuti Astuti, et al. "The Influence of Palm Oil-Based Zinc Stearate Application on Vulcanized Rubber: Analyzing Hardness, Tensile Strength, and Elongation at Break for Hand Grip Formulation Development." Key Engineering Materials 1001 (December 18, 2024): 111–17. https://doi.org/10.4028/p-wc8yqp.

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Vulcanization of rubber compounds is a crucial step in the process of developing natural rubber end products. Selected components are then used to formulate a rubber compound, which is further treated to create vulcanized rubber. In this study, a palm oil-based zinc stearate compound (ZS) developed by the National Research and Innovation Agency (BRIN) was used as a replacement for ZnO and stearic acid, which were previously used as activator and co-activator in the process of vulcanization. The goal of this study was to create a compound made of rubber formulation for use in the hand grip. The

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Figueroa, Eric E., and Jerod S. Denton. "Zinc pyrithione activates the volume-regulated anion channel through an antioxidant-sensitive mechanism." American Journal of Physiology-Cell Physiology 320, no. 6 (2021): C1088—C1098. http://dx.doi.org/10.1152/ajpcell.00070.2021.

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Leucine-rich repeat-containing 8 (LRRC8) volume-regulated anion channels (VRACs) play important physiological roles in diverse cell types and may represent therapeutic targets for various diseases. To date, however, the pharmacological tools for evaluating the druggability of VRACs have been limited to inhibitors, as no activators of the channel have been reported. We therefore performed a fluorescence-based high-throughput screening (HTS) of 1,184 Food and Drug Administration-approved drugs for compounds that increase VRAC activity. The most potent VRAC potentiator identified was zinc pyrithi

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Sunkara, Jhansi R., and Sathish M. Botsa. "Facile Synthesis of 1,8-dioxooctahydro Xanthenes by Reusable Zinc Sulfide based Ternary Nanocomposite via Hydrothermal Route." Current Catalysis 9, no. 1 (2020): 72–79. http://dx.doi.org/10.2174/2211544708666191112120505.

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Background: Metal oxide or metal oxide composite nanoparticles are attaining tremendous importance due to their catalytic activities for various organic transformations. Objective: To report the one-pot synthesis of xanthene derivatives prepared by ZnS-Fe2O3-Ag composite under solvent-free conditions. Method: To prepare nanocomposite by a facile and simple hydrothermal approach. Results: The prepared composite is smaller (17.56 nm) in size and can be easily separable, recycled and reused six times without any significant loss of catalytic activity with excellent yields. In short reaction time,

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Ajekwene, Kingsley Kema, Johnson Oseghale Oboh, Ugonna Kingsley Ugo, and Simon Ikechukwu Ichetaonye. "Preparation of Multifunctional Additive [MFA] from Castor Oil (CO) and Study of its Effects on the Physical Properties of Natural Rubber Vulcanizates." International Journal of Research in Advent Technology 11, no. 3 (2023): 1–10. http://dx.doi.org/10.32622/ijrat.111202311.

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This experiment focuses on the synthesis of Multifunctional Additives (MFA) from Castor seed oil (CO) and pure Oleic acid (OA) respectively. These multifunctional additives (CO-MFA and OA-MFA) were investigated as potential substitutes for process oil, activator, co-activator, and accelerator in the formulation of a natural rubber vulcanizate. During processing, the castor seed oil was characterized for acid value (AV), free fatty acid (FFA) and saponification value (SV) and subsequently reacted with 1, 6-hexane diamine to obtain a mixture of salts termed castor oil-based multifunctional addit

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Xie, Zhigang, Wei Guan, Fangying Ji, Zhongrong Song, and Yanling Zhao. "Production of Biologically Activated Carbon from Orange Peel and Landfill Leachate Subsequent Treatment Technology." Journal of Chemistry 2014 (2014): 1–9. http://dx.doi.org/10.1155/2014/491912.

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In order to improve adsorption of macromolecular contaminants and promote the growth of microorganisms, active carbon for biological wastewater treatment or follow-up processing requires abundant mesopore and good biophile ability. In this experiment, biophile mesopore active carbon is produced in one-step activation with orange peel as raw material, and zinc chloride as activator, and the adsorption characteristics of orange peel active carbon is studied by static adsorption method. BET specific surface area and pore volume reached 1477 m2/g and 2.090 m3/g, respectively. The surface functiona

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Alam, Md Najib, Vineet Kumar, and Sang-Shin Park. "Advances in Rubber Compounds Using ZnO and MgO as Co-Cure Activators." Polymers 14, no. 23 (2022): 5289. http://dx.doi.org/10.3390/polym14235289.

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Zinc oxide performs as the best cure activator in sulfur-based vulcanization of rubber, but it is regarded as a highly toxic material for aquatic organisms. Hence, the toxic cure activator should be replaced by a non-toxic one. Still, there is no suitable alternative industrially. However, binary activators combining ZnO and another metal oxide such as MgO can largely reduce the level of ZnO with some improved benefits in the vulcanization of rubber as investigated in this research. Curing, mechanical, and thermal characteristics were investigated to find out the suitability of MgO in the vulc

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Troconis de Rincón, Oladis, Andrés Torres-Acosta, Alberto Sagüés, and Miguel Martinez-Madrid. "Galvanic Anodes for Reinforced Concrete Structures: A Review." Corrosion 74, no. 6 (2018): 715–23. http://dx.doi.org/10.5006/2613.

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In recent years, the use of sacrificial anodes for cathodic protection in reinforced concrete structures has increased, reflecting ease of installation, low-maintenance requirements, as well as desirability in prestressed concrete structures where the naturally controlled protection potential decreases the risk of hydrogen embrittlement. Zinc-based alloys have been among the most evaluated galvanic materials for concrete structures, especially in the United States, in many applications: thermal spray, superficial metal/mesh with and without hydrogel adhesive, embedded in concrete (point anodes

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Petrova A. V., Sidorova A. V., Sergeeva I. A., and Petrova G. P. "Evaluation of the influence of chromium and zinc ions on the collagenolysis process in solutions by the method of dynamic light scattering." Optics and Spectroscopy 130, no. 6 (2022): 663. http://dx.doi.org/10.21883/eos.2022.06.54701.23-22.

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Using the method of dynamic light scattering, it was found that the rate of collagen cleavage under the influence of bacterial collagenase decreases by almost 4 times when protein molecules are doped with Cr3+ ions, and when the enzyme activator of Zn2+ ions is added, it increases by 1.3 times. Based on the experimental data, the reaction rate constants k1 were calculated. It was found that when collagen molecules are tanned with a chromium salt, it becomes more resistant to degradation in solutions containing zinc ions than in solutions with the addition of calcium ions, as we described in ea

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Darmayanti, Lita, Suprihanto Notodarmojo, Enri Damanhuri, Grandprix T. M. Kadja, and Rino R Mukti. "Preparation of alkali-activated fly ash-based geopolymer and their application in the adsorption of copper (II) and zinc (II) ions." MATEC Web of Conferences 276 (2019): 06012. http://dx.doi.org/10.1051/matecconf/201927606012.

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Alkali activation of fly ash can a promising alternative of the system to improve adsorption capability of fly ash. In finding the best chemical composition of the activator solution, geopolymer has been synthesized using molar ratios of Na2O/SiO2 0.16, 0.3, and 0.5 (Gr1, Gr3, Gr5). The results indicated that the geopolymer synthesized with a ratio molar of Na2O/SiO2 0.3 (Gr3) improved the adsorption properties of fly ash substantially. Gr3 was characterized by BET, XRD, and FTIR. The batch experiment was conducted at the different duration and initial concentrations. The equilibrium sorption

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Xu, Wenlina. "Research progress of gene editing technology CRISPR/Cas9 system in animal gene editing." International Journal of Veterinary Science and Research 4, no. 1 (2018): 015–19. https://doi.org/10.17352/ijvsr.000030.

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Gene editing technology, from the beginning of RNA interference (RNAi) technology to efficient developed enzyme technology, has been widely used in recent years. These efficient enzyme technologies include zinc finger nuclease (ZFN) technology, transcriptional activation-like effector nuclease (TALENs) technology, and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) 9 system (CRISPR/Cas9) technology. The CRISPR/Cas (Cas) system is a gene editing tool for DNA modification regulated by a short RNA and is a new type of genome editing tool that is faster,

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Motorina, Daria M., Yuliya A. Galimova, Nadezhda V. Battulina, and Evgeniya S. Omelina. "Systems for Targeted Silencing of Gene Expression and Their Application in Plants and Animals." International Journal of Molecular Sciences 25, no. 10 (2024): 5231. http://dx.doi.org/10.3390/ijms25105231.

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At present, there are a variety of different approaches to the targeted regulation of gene expression. However, most approaches are devoted to the activation of gene transcription, and the methods for gene silencing are much fewer in number. In this review, we describe the main systems used for the targeted suppression of gene expression (including RNA interference (RNAi), chimeric transcription factors, chimeric zinc finger proteins, transcription activator-like effectors (TALEs)-based repressors, optogenetic tools, and CRISPR/Cas-based repressors) and their application in eukaryotes—plants a

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Borislav, N. Malinovic, Zoric Dusko, and Djuricic Tijana. "Corrosion coupon testing of commercial inhibitor in simulated cooling water." Technologica Acta 13, no. 1 (2020): 27–32. https://doi.org/10.5281/zenodo.4059952.

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In maintaining cooling systems, one of the biggest challenges is to control the corrosion process. Various corrosion inhibitors are often used for this purpose. Which type of corrosion inhibitor will be chosen depends on the material from which the plant has made. The main causes of corrosion in these systems are: pH, dissolved gas, ammonia, temperature and microbiology. In this paper it was studied the efficiency of two multicomponent commercial corrosion inhibitors based on phosphates and one of which containing zinc chloride. For the purposes of research, the pilot plant of open recirculati

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Cahill, Catherine M., Sanjan S. Sarang, Rachit Bakshi, Ning Xia, Debomoy K. Lahiri, and Jack T. Rogers. "Neuroprotective Strategies and Cell-Based Biomarkers for Manganese-Induced Toxicity in Human Neuroblastoma (SH-SY5Y) Cells." Biomolecules 14, no. 6 (2024): 647. http://dx.doi.org/10.3390/biom14060647.

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Manganese (Mn) is an essential heavy metal in the human body, while excess Mn leads to neurotoxicity, as observed in this study, where 100 µM of Mn was administered to the human neuroblastoma (SH-SY5Y) cell model of dopaminergic neurons in neurodegenerative diseases. We quantitated pathway and gene changes in homeostatic cell-based adaptations to Mn exposure. Utilizing the Gene Expression Omnibus, we accessed the GSE70845 dataset as a microarray of SH-SY5Y cells published by Gandhi et al. (2018) and applied statistical significance cutoffs at p < 0.05. We report 74 pathway and 10 gene chang

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Wallis, Christopher P., Louis H. Scott, Aleksandra Filipovska, and Oliver Rackham. "Manipulating and elucidating mitochondrial gene expression with engineered proteins." Philosophical Transactions of the Royal Society B: Biological Sciences 375, no. 1790 (2019): 20190185. http://dx.doi.org/10.1098/rstb.2019.0185.

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Many conventional, modern genome engineering tools cannot be used to study mitochondrial genetics due to the unusual structure and physiology of the mitochondrial genome. Here, we review a number of newly developed, synthetic biology-based approaches for altering levels of mutant mammalian mitochondrial DNA and mitochondrial RNAs, including transcription activator-like effector nucleases, zinc finger nucleases and engineered RNA-binding proteins. These approaches allow researchers to manipulate and visualize mitochondrial processes and may provide future therapeutics. This article is part of t

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Männistö, Riina H., A. Marika Grahn, Dennis H. Bamford, and Jaana K. H. Bamford. "Transcription of Bacteriophage PM2 Involves Phage-Encoded Regulators of Heterologous Origin." Journal of Bacteriology 185, no. 11 (2003): 3278–87. http://dx.doi.org/10.1128/jb.185.11.3278-3287.2003.

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ABSTRACT Bacteriophage PM2 is the only described member of the Corticoviridae family. It is an icosahedral dsDNA virus with a membrane residing underneath the protein coat. PM2 infects some gram-negative Pseudoalteromonas spp. In the present study, we mapped the viral promoters and showed that the PM2 genome consists of three operons. Four new virus genes were assigned based on their function in transcription. Proteins P15 and P16 are shown to repress early transcription, and proteins P13 and P14 are shown to activate late transcription events. The early regulatory region, containing genes for

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Xu, Tao, Yongchao Li, Joy D. Van Nostrand, Zhili He, and Jizhong Zhou. "Cas9-Based Tools for Targeted Genome Editing and Transcriptional Control." Applied and Environmental Microbiology 80, no. 5 (2014): 1544–52. http://dx.doi.org/10.1128/aem.03786-13.

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ABSTRACTDevelopment of tools for targeted genome editing and regulation of gene expression has significantly expanded our ability to elucidate the mechanisms of interesting biological phenomena and to engineer desirable biological systems. Recent rapid progress in the study of a clustered, regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated (Cas) protein system in bacteria has facilitated the development of newly facile and programmable platforms for genome editing and transcriptional control in a sequence-specific manner. The core RNA-guided Cas9 endonuclease in the type

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Pu, Mengjie, Daqi Ye, Jinquan Wan, Bentuo Xu, Wei Sun, and Wei Li. "Zinc-based metal–organic framework nanofibers membrane ZIF-65/PAN as efficient peroxymonosulfate activator to degrade aqueous ciprofloxacin." Separation and Purification Technology 299 (October 2022): 121716. http://dx.doi.org/10.1016/j.seppur.2022.121716.

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Петрова, А. В., А. В. Сидорова, И. А. Сергеева та Г. П. Петрова. "Оценка влияния ионов хрома и цинка на процесс коллагенолиза в растворах методом динамического рассеяния света". Оптика и спектроскопия 130, № 6 (2022): 838. http://dx.doi.org/10.21883/os.2022.06.52624.23-22.

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Using the method of dynamic light scattering, it was found that the rate of collagen cleavage under the influence of bacterial collagenase decreases by almost 4 times when protein molecules are doped with Cr3+ ions, and when the enzyme activator of Zn2+ ions is added, it increases by 1.3 times. Based on the experimental data, the reaction rate constants k1 were calculated. It was found that when collagen molecules are tanned with a chromium salt, it becomes more resistant to degradation in solutions containing zinc ions than in solutions with the addition of calcium ions, as we described in ea

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Sandalov, S. I., N. F. Ushmarin, N. A. Chernova, and N. I. Kol'tsov. "Investigating Zinc Methacrylate as An activator of the Peroxide vulcanisation of A Rubber Mix Based on Nitrile Butadiene Rubber." International Polymer Science and Technology 40, no. 11 (2013): T25—T27. http://dx.doi.org/10.1177/0307174x1304001110.

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Riggs, K. J., S. Saleque, K. K. Wong, et al. "Yin-yang 1 activates the c-myc promoter." Molecular and Cellular Biology 13, no. 12 (1993): 7487–95. http://dx.doi.org/10.1128/mcb.13.12.7487-7495.1993.

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Previous studies on the murine c-myc promoter demonstrated that a ubiquitously present protein, common factor 1 (CF1), bound at two sites located -260 and -390 bp from the P1 transcription start site. CF1 has been purified to near homogeneity and shown to be identical to the zinc finger protein Yin-yang 1 (YY1) as judged by similarity of molecular weight and other biochemical properties, immunological cross-reactivity, and the ability of recombinant YY1 to bind to CF1 sites. In cotransfection experiments, YY1 is a strong activator of transcription from c-myc promoter-based reporters. Furthermo

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Riggs, K. J., S. Saleque, K. K. Wong, et al. "Yin-yang 1 activates the c-myc promoter." Molecular and Cellular Biology 13, no. 12 (1993): 7487–95. http://dx.doi.org/10.1128/mcb.13.12.7487.

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Previous studies on the murine c-myc promoter demonstrated that a ubiquitously present protein, common factor 1 (CF1), bound at two sites located -260 and -390 bp from the P1 transcription start site. CF1 has been purified to near homogeneity and shown to be identical to the zinc finger protein Yin-yang 1 (YY1) as judged by similarity of molecular weight and other biochemical properties, immunological cross-reactivity, and the ability of recombinant YY1 to bind to CF1 sites. In cotransfection experiments, YY1 is a strong activator of transcription from c-myc promoter-based reporters. Furthermo

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Taghvaei-Ganjali, Saeed, Mercedeh Malekzadeh, Mona Farahani, Ali Abbasian, and Morteza khosravi. "Effect of surface-modified zinc oxide as cure activator on the properties of a rubber compound based on NR/SBR." Journal of Applied Polymer Science 122, no. 1 (2011): 249–56. http://dx.doi.org/10.1002/app.33919.

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Pang, Xie Guan, Tien Yew Eeu, Pau Ming Leong, Wan Nurulhuda Wan Shamsuri, and Rosli Hussin. "Structural and Luminescence Study of Rare Earth and Transition Metal Ions Doped Lead Zinc Borophosphate Glasses." Advanced Materials Research 895 (February 2014): 280–83. http://dx.doi.org/10.4028/www.scientific.net/amr.895.280.

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A series of glasses with composition of xPbO-(50-x)ZnO-yB2O3-(50-y)P2O5 with 0 x 50 mol% and 10 y 20 mol% were prepared by melt quenching technique, with 30 minutes pre-heating and 10 minutes for melting. The structural properties of prepared samples were studied using Fourier Transform-Infrared spectroscopy. The glasses were mainly based on PO2, BOP and BO3 unit. In order to obtain luminescence properties, another series of sample at composition 20PbO-30ZnO-10B2O3-40P2O5 doped with Fe2+, Ti2+, Y2+ and Nd2+ were prepared. These samples were investigated using Photoluminescence Spectroscopy wit

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Xu, Xiao Yan, Guo Tong Qian, Jian Zhou, Yao Yao, and Xu Chen. "Wettability of Sn-Zn Lead-Free Solder on Aluminum Substrate." Advanced Materials Research 815 (October 2013): 48–54. http://dx.doi.org/10.4028/www.scientific.net/amr.815.48.

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The effects of flux components and compositions of solder alloys on the wettability of the Sn-Zn alloys on aluminum surface was investigated. The results show that the wettability of the Sn-9Zn solder on aluminum substrate improved with flux of double solvents composed of diethanolamine and triethanolamine, which is better than single solvent. When flux is composed of 3% zinc fluoborate as activator and 30% triethanolamine plus 67% diethanolamine as double solvents, the spreading area of the Sn-9Zn solder reaches to 75%. Trace addition (0.002%-0.005%) of Al results in significant improvement o

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Kenzhalievich, Kenzhaliyev Bagdaulet, Koizhanova Aigul Kairgeldyevna, Magomedov David Rasimovich, et al. "A Study on the efficiency of organic activator application to process refractory hard-to-beneficiate raw materials." Jurnal Pendidikan Teknologi Kejuruan 7, no. 3 (2024): 185–203. http://dx.doi.org/10.24036/jptk.v7i3.38323.

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Polymetallic ores with complex mineralogical compositions pose significant challenges in flotation beneficiation, often resulting in suboptimal separation and recovery of valuable minerals. This study aims to analyse the effectiveness of alkyl benzene sulfonic acid (ABSA) as a frothing activator in improving the flotation performance and mineral recovery of polymetallic ore. It presents the results of flotation beneficiation experiments for polymetallic ore of complex mineralogical composition. Experiments were conducted on polymetallic ore samples using ABSA as the frothing activator in the f

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Konjevic, Gordana, and Sandra Stankovic. "Matrix metalloproteinases in the process of invasion and metastasis of breast cancer." Archive of Oncology 14, no. 3-4 (2006): 136–40. http://dx.doi.org/10.2298/aoo0604136k.

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Metastatic cascade in malignant tumors, including breast cancer, starts with localized invasion of the host tissue. This process, requiring that tumor cells separate from each other, includes loss of homotypic and heterotypic cell adhesion and cell-cell contact inhibition, acquisition of motility, exacerbated by "epithelial-to-mesenchymal transition", and production of proteolytic enzymes which degrade basal membrane and extracellular matrix. In this sense, aside from urokinase type plasminogen activator, increased expression and activity of matrix metalloproteinases (MMPs) is one of the earli

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Canver, Matthew C., та Stuart H. Orkin. "Customizing the genome as therapy for the β-hemoglobinopathies". Blood 127, № 21 (2016): 2536–45. http://dx.doi.org/10.1182/blood-2016-01-678128.

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Abstract Despite nearly complete understanding of the genetics of the β-hemoglobinopathies for several decades, definitive treatment options have lagged behind. Recent developments in technologies for facile manipulation of the genome (zinc finger nucleases, transcription activator-like effector nucleases, or clustered regularly interspaced short palindromic repeats–based nucleases) raise prospects for their clinical application. The use of genome-editing technologies in autologous CD34+ hematopoietic stem and progenitor cells represents a promising therapeutic avenue for the β-globin disorder

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Phan, Hong Thi Lam, Kyoungmi Kim, Ho Lee, and Je Kyung Seong. "Progress in and Prospects of Genome Editing Tools for Human Disease Model Development and Therapeutic Applications." Genes 14, no. 2 (2023): 483. http://dx.doi.org/10.3390/genes14020483.

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Programmable nucleases, such as zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and clustered regularly interspaced short palindromic repeats (CRISPR)/Cas, are widely accepted because of their diversity and enormous potential for targeted genomic modifications in eukaryotes and other animals. Moreover, rapid advances in genome editing tools have accelerated the ability to produce various genetically modified animal models for studying human diseases. Given the advances in gene editing tools, these animal models are gradually evolving toward mimicking hum

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Radkowski, Adam, Iwona Radkowska, Tadeusz Lemek, and Tomasz Jakubowski. "Effect of Zinc Ammonium Acetate on Characteristics of Timothy Canopy and Seed Yield." Ecological Chemistry and Engineering S 26, no. 4 (2019): 797–806. http://dx.doi.org/10.1515/eces-2019-0056.

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Abstract The purpose of the experiment was to assess the effect of application of zinc ammonium acetate (ZAA) on yielding, morphological features and on selected vegetation indices of timothy cv. ‘Owacja’ cultivated for seeds. Zinc ammonium acetate that has a biostimulatory effect was used foliar in the carried out experiment. The experiment was conducted in the years 2015-2017 at the experimental station in Prusy near Krakow, a part of the Experimental Station of the Institute of Crop Production of the University of Agriculture in Krakow. The field experiment was set up in a randomized block

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Urbano, Smith, Weeks, and Chatterjee. "Gene-Specific Targeting of DNA Methylation in the Mammalian Genome." Cancers 11, no. 10 (2019): 1515. http://dx.doi.org/10.3390/cancers11101515.

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DNA methylation is the most widely-studied epigenetic modification, playing a critical role in the regulation of gene expression. Dysregulation of DNA methylation is implicated in the pathogenesis of numerous diseases. For example, aberrant DNA methylation in promoter regions of tumor-suppressor genes has been strongly associated with the development and progression of many different tumors. Accordingly, technologies designed to manipulate DNA methylation at specific genomic loci are very important, especially in the context of cancer therapy. Traditionally, epigenomic editing technologies hav

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Wu, Yanling, Qinggao Hou, Fangzhou Li, et al. "Mitigating Co Metal Particle Agglomeration and Enhancing ORR Catalytic Activity through Nitrogen-Enriched Porous Carbon Derived from Biomass." Catalysts 13, no. 7 (2023): 1118. http://dx.doi.org/10.3390/catal13071118.

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Biomass-derived porous carbon has gained significant attention as a cost-effective and sustainable material in non-noble metal carbon-based electrocatalysts for the oxygen reduction reaction (ORR). However, during the preparation of transition metal catalysts based on biomass-derived porous carbon, the agglomeration of transition metal atoms often occurs, leading to a notable decline in catalytic activity. In this study, we present a straightforward synthetic approach for the preparation of nitrogen-enriched soybean-derived porous carbon (Co@SP-C-a) as an electrocatalyst for the ORR. To achiev

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Schreurs, Juliët, Claudia Sacchetto, Robin M. W. Colpaert, Libero Vitiello, Alessandra Rampazzo, and Martina Calore. "Recent Advances in CRISPR/Cas9-Based Genome Editing Tools for Cardiac Diseases." International Journal of Molecular Sciences 22, no. 20 (2021): 10985. http://dx.doi.org/10.3390/ijms222010985.

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In the past two decades, genome editing has proven its value as a powerful tool for modeling or even treating numerous diseases. After the development of protein-guided systems such as zinc finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs), which for the first time made DNA editing an actual possibility, the advent of RNA-guided techniques has brought about an epochal change. Based on a bacterial anti-phage system, the CRISPR/Cas9 approach has provided a flexible and adaptable DNA-editing system that has been able to overcome several limitations associated wi

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Cheng, Yangfan, Sirui Zhang, and Huifang Shang. "Latest advances on new promising molecular-based therapeutic approaches for Huntington’s disease." Journal of Translational Internal Medicine 12, no. 2 (2024): 134–47. http://dx.doi.org/10.2478/jtim-2023-0142.

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Abstract Huntington’s disease (HD) is a devastating, autosomal-dominant inherited, neurodegenerative disorder characterized by progressive motor deficits, cognitive impairments, and neuropsychiatric symptoms. It is caused by excessive cytosine-adenine-guanine (CAG) trinucleotide repeats within the huntingtin gene (HTT). Presently, therapeutic interventions capable of altering the trajectory of HD are lacking, while medications for abnormal movement and psychiatric symptoms are limited. Numerous pre-clinical and clinical studies have been conducted and are currently underway to test the efficac

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Yano, Naohiro, and Alexey V. Fedulov. "Targeted DNA Demethylation: Vectors, Effectors and Perspectives." Biomedicines 11, no. 5 (2023): 1334. http://dx.doi.org/10.3390/biomedicines11051334.

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Aberrant DNA hypermethylation at regulatory cis-elements of particular genes is seen in a plethora of pathological conditions including cardiovascular, neurological, immunological, gastrointestinal and renal diseases, as well as in cancer, diabetes and others. Thus, approaches for experimental and therapeutic DNA demethylation have a great potential to demonstrate mechanistic importance, and even causality of epigenetic alterations, and may open novel avenues to epigenetic cures. However, existing methods based on DNA methyltransferase inhibitors that elicit genome-wide demethylation are not s

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Beil, J., L. Fairbairn, P. Pelczar, and T. Buch. "Is BAC Transgenesis Obsolete? State of the Art in the Era of Designer Nucleases." Journal of Biomedicine and Biotechnology 2012 (2012): 1–5. http://dx.doi.org/10.1155/2012/308414.

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DNA constructs based on bacterial artificial chromosomes (BACs) are frequently used to generate transgenic animals as they reduce the influence of position effects and allow predictable expression patterns for genes whose regulatory sequences are not fully identified. Despite these advantages BAC transgenics suffer from drawbacks such as complicated vector construction, low efficiency of transgenesis, and some remaining expression variegation. The recent development of transcription activator-like effector nucleases (TALENs) and zinc finger nucleases (ZFNs) has resulted in new transgenic techn

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Khamaganova, Tatyana N. "Preparation, luminescence, and application of LiMeBO3 borates, Me = Mg, Ca, Sr, Ba, Zn, Cd. Review." Kondensirovannye sredy i mezhfaznye granitsy = Condensed Matter and Interphases 25, no. 3 (2023): 311–32. http://dx.doi.org/10.17308/kcmf.2023.25/11256.

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The review summarises and analyses data on the preparation, structure, and spectral-luminescent properties of LiMeBO3-based borates, Me = bivalent metal. These polycrystalline borates are prepared traditionally by solid-phase reactions and self-propagating high-temperature synthesis and its modifications based on a combustion reaction. Frameworks of lithium borates with alkaline earth metals, zinc, and cadmium are formed from large metal polyhedra between which there are boron-oxygen triangles isolated from each other. Doping with rare-earth and heavy metal ions leads to the formation of solid

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Ortiz-Vitali, Jose L., and Radbod Darabi. "iPSCs as a Platform for Disease Modeling, Drug Screening, and Personalized Therapy in Muscular Dystrophies." Cells 8, no. 1 (2019): 20. http://dx.doi.org/10.3390/cells8010020.

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Induced pluripotent stem cells (iPSCs) are the foundation of modern stem cell-based regenerative medicine, especially in the case of degenerative disorders, such as muscular dystrophies (MDs). Since their introduction in 2006, many studies have used iPSCs for disease modeling and identification of involved mechanisms, drug screening, as well as gene correction studies. In the case of muscular dystrophies, these studies commenced in 2008 and continue to address important issues, such as defining the main pathologic mechanisms in different types of MDs, drug screening to improve skeletal/cardiac

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Thanh, Nguyen Duc. "Application of genome editing tools in plants." Vietnam Journal of Biotechnology 19, no. 1 (2021): 15–40. http://dx.doi.org/10.15625/1811-4989/15464.

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Genome editing technology is the genome modification techniques, such as targeted mutagenesis or insert/delete/replacement at specific locations in the genome of living organisms. Genome editing is based on the creation of double sequence break (DSB) in a specific location and DNA repair via nonhomologous end joining (NHEJ) or homology direct repair (HDR). The development of sequence-specific nuclease (SSN) allows precise editing of the target gene. These SSNs include: meganuclease (MN), zinc finger nuclease (ZFN), transcription activator-like effector nuclease (TALEN) and CRISPR-associated nu

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Kim, Daesik, Kevin Luk, Scot A. Wolfe, and Jin-Soo Kim. "Evaluating and Enhancing Target Specificity of Gene-Editing Nucleases and Deaminases." Annual Review of Biochemistry 88, no. 1 (2019): 191–220. http://dx.doi.org/10.1146/annurev-biochem-013118-111730.

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Programmable nucleases and deaminases, which include zinc-finger nucleases, transcription activator-like effector nucleases, CRISPR RNA-guided nucleases, and RNA-guided base editors, are now widely employed for the targeted modification of genomes in cells and organisms. These gene-editing tools hold tremendous promise for therapeutic applications. Importantly, these nucleases and deaminases may display off-target activity through the recognition of near-cognate DNA sequences to their target sites, resulting in collateral damage to the genome in the form of local mutagenesis or genomic rearran

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Pang, Jiahui. "Research Progress in The Application of CRISPR Gene Editing Technology in Virus Detection and Treatment." Highlights in Science, Engineering and Technology 45 (April 18, 2023): 302–7. http://dx.doi.org/10.54097/hset.v45i.7446.

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Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) gene editing (GE) technique is the latest generation of GE technology, which can carry out targeted and precise modification of the genome. In this article, a comparison of the Zinc-Finger Nucleases (ZFNs) , CRISPR/Cas and Transcription Activator-Like Effector Nucleases (TALENs) is presented, which indicates that CRISPR/Cas has significant advantages. For example, synthesis and screening are simpler and faster, and multiple genes can be edited simultaneously. To keep abreast of the times, current progress in the application of

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Geel, T. M., M. H. J. Ruiters, R. H. Cool, et al. "The past and presence of gene targeting: from chemicals and DNA via proteins to RNA." Philosophical Transactions of the Royal Society B: Biological Sciences 373, no. 1748 (2018): 20170077. http://dx.doi.org/10.1098/rstb.2017.0077.

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The ability to target DNA specifically at any given position within the genome allows many intriguing possibilities and has inspired scientists for decades. Early gene-targeting efforts exploited chemicals or DNA oligonucleotides to interfere with the DNA at a given location in order to inactivate a gene or to correct mutations. We here describe an example towards correcting a genetic mutation underlying Pompe's disease using a nucleotide-fused nuclease (TFO-MunI). In addition to the promise of gene correction, scientists soon realized that genes could be inactivated or even re-activated witho

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Baya, Georgina, Stephen Muhindi, Valentine Ngendahimana, and Jonathan Caguiat. "Potential Whole-Cell Biosensors for Detection of Metal Using MerR Family Proteins from Enterobacter sp. YSU and Stenotrophomonas maltophilia OR02." Micromachines 12, no. 2 (2021): 142. http://dx.doi.org/10.3390/mi12020142.

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Cell-based biosensors harness a cell’s ability to respond to the environment by repurposing its sensing mechanisms. MerR family proteins are activator/repressor switches that regulate the expression of bacterial metal resistance genes and have been used in metal biosensors. Upon metal binding, a conformational change switches gene expression from off to on. The genomes of the multimetal resistant bacterial strains, Stenotrophomonas maltophilia Oak Ridge strain 02 (S. maltophilia 02) and Enterobacter sp. YSU, were recently sequenced. Sequence analysis and gene cloning identified three mercury r

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Psatha, Nikoletta, Kiriaki Paschoudi, Anastasia Papadopoulou, and Evangelia Yannaki. "In Vivo Hematopoietic Stem Cell Genome Editing: Perspectives and Limitations." Genes 13, no. 12 (2022): 2222. http://dx.doi.org/10.3390/genes13122222.

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The tremendous evolution of genome-editing tools in the last two decades has provided innovative and effective approaches for gene therapy of congenital and acquired diseases. Zinc-finger nucleases (ZFNs), transcription activator- like effector nucleases (TALENs) and CRISPR-Cas9 have been already applied by ex vivo hematopoietic stem cell (HSC) gene therapy in genetic diseases (i.e., Hemoglobinopathies, Fanconi anemia and hereditary Immunodeficiencies) as well as infectious diseases (i.e., HIV), and the recent development of CRISPR-Cas9-based systems using base and prime editors as well as epi

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Bondareva, Olga, Roman Tsaryk, Vesna Bojovic, Maria Odenthal-Schnittler, Arndt F. Siekmann, and Hans-J. Schnittler. "Identification of atheroprone shear stress responsive regulatory elements in endothelial cells." Cardiovascular Research 115, no. 10 (2019): 1487–99. http://dx.doi.org/10.1093/cvr/cvz027.

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Abstract Aims Oscillatory shear stress (OSS) is an atheroprone haemodynamic force that occurs in areas of vessel irregularities and is implicated in the pathogenesis of atherosclerosis. Changes in signalling and transcriptional programme in response to OSS have been vigorously studied; however, the underlying changes in the chromatin landscape controlling transcription remain to be elucidated. Here, we investigated the changes in the regulatory element (RE) landscape of endothelial cells under atheroprone OSS conditions in an in vitro model. Methods and results Analyses of H3K27ac chromatin im

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Yu, N., J. Yang, Y. Mishina, and W. V. Giannobile. "Genome Editing: A New Horizon for Oral and Craniofacial Research." Journal of Dental Research 98, no. 1 (2018): 36–45. http://dx.doi.org/10.1177/0022034518805978.

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Precise and efficient genetic manipulations have enabled researchers to understand gene functions in disease and development, providing a platform to search for molecular cures. Over the past decade, the unprecedented advancement of genome editing techniques has revolutionized the biological research fields. Early genome editing strategies involved many naturally occurring nucleases, including meganucleases, zinc finger nucleases, and transcription activator-like effector-based nucleases. More recently, the clustered regularly interspaced short palindromic repeats (CRISPR) / CRISPR-associated

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