Littérature scientifique sur le sujet « Yi feng tang »

Background:Autophagy is a phenomenon of “self-phagocytosis” in eukaryotic cells, which maintains cell homeostasis by transporting intracellular materials to lysosomes for degradation and recycling. In recent years, studies have shown that autophagy may be involved in the pathogenesis of rheumatoid arthritis(RA)[1], but its specific mechanism is still unclear.Objectives:The expression levels of autophagy-related genes(ATG) unc-51-like kinase 1(ULK1), ATG13, ATG17, microtubule associated protein 1 light chain 3 (LC3), and P62 in peripheral blood mononuclear cells (PBMC) of patients with RA were detected, and their role and clinical significance in the pathogenesis of RA were explored.Methods:Real-time fluorescent quantitative PCR was performed to detect the expression levels of ULK1, ATG13, ATG17, LC3, and P62 in PBMCs of 50 RA patients, 50 healthy controls (HC), and 25 moderate to severe RA patients before and after treatment. Then, t test, χ2 test, Mann-Whitney U test, Pearson test were used for statistical analysis.Results:1.The levels of hsCRP, white blood cell(WBC), neutrophils(GR), platelet(PLT) and plateletcrit(PCT) in RA group were higher than those in HC group (P <0.05). Lymphocytes (LY), red blood cell(RBC), hemoglobin(HGB), hematocrit(HCT), mean corpuscular hemoglobin(MCH), mean red blood cell volume(MCV) and mean red blood cell hemoglobin concentration(MCHC) in RA group were lower than those in HC group (P <0.05). 2.The expressions of ULK1, ATG17, and LC3 in RA group were higher than those in HC group, while the expressions of P62 was lower than those in HC group(P<0.05) (Figure 1). The correlation analysis suggested that ATG17 was positively correlated with tender joint count (TJC), swollen joint count (SJC), and health assessment questionnaire (HAQ) (P<0.05); ULK1 and HAQ were negatively correlated (P<0.05).3. Compared with before treatment with TNFi, ATG17, HAQ, DAS-28, ESR, hsCRP, WBC, GR, PLT and PCT were significantly reduced after treatment (P<0.05); the expressions of RBC, HCT, MCV and MCH were significantly increased after treatment,(P<0.05); ULK1, ATG13, LC3, P62 and other related clinical and laboratory indicators were not significantly different before and after treatment with TNFi (P>0.05).Figure 1.The expression levels of ATGs in HC and RA groups.Conclusion:There is abnormal expression of autophagy genes in the peripheral blood of RA patients. ULK1, ATG17, LC3 and P62 may be related to the pathogenesis of RA, among them, ATG17 may regulate the pathogenesis of RA by participating in the TNF-α pathway.References:[1]Rockel Jason S,Kapoor Mohit,Autophagy: controlling cell fate in rheumatic diseases.[J].Nat Rev Rheumatol, 2016, 12: 517-31.Disclosure of Interests:Yu-Feng Qing Grant/research support from: Science and Technology Project of Nanchong City (no.18SXHZ0522), Fei Dai: None declared, Quan-Bo Zhang Grant/research support from: the National Natural Science Foundation of China(General Program) (no.81974250), and Science and Technology Plan Project of Sichuan Province (no.2018JY0257), Yi-Ping Tang: None declared, Zeng-Rong Dong: None declared, Yi-Xi He: None declared, Yi Jiang: None declared, Yu-Qin Huang: None declared, Jianxiong Zheng: None declared

Background:Gout is an arthritic disease caused by the deposition of monosodium urate crystal (MSU) in the joints, which can lead to acute inflammation and damage adjacent tissue [1].Over the past decade, noncoding RNAs (ncRNAs) have been shown to have crucial importance in health and disease[2,3]. However, studies evaluating the function of ncRNAs in gout are scarce, and current knowledge of the role of ncRNAs in gout is still limited.Objectives:To assess the contribution of noncoding RNAs to gout and the clinical importance of these genes in primary gouty arthritis (GA).Methods:The mRNA expression levels of noncoding RNAs (LINC00173, LINC00963, LINC01330 and miRNA-182-5p) were measured in peripheral blood mononuclear cells (PBMCs) from 60 gout patients(including 30 acute gout patients, 30 intercritical gout patients) and 40 healthy subjects. The relationship between noncoding RNA expression levels and laboratory features was analyzed in GA patients.Results:The expression levels of LINC00173, LINC00963 and miRNA-182-5p were much lower in the AG and IG group than in the HC groups (p<0.05), and no significant difference was detected between AG and IG groups(P>0.05). The expression levels of LINC01330 were much lower in the AG group than in the IG and HC groups (p<0.05), and no significant difference was detected between AG and IG groups(P>0.05). In GA patients, the levels of noncoding RNAs mRNA correlated with laboratory inflammatory and metabolic indexes.Conclusion:Altered noncoding RNAs expression suggests that noncoding RNAs is involved in the pathogenesis of GA and participates in regulating inflammation and metabolism.References:[1]Xu Yi-Ting,Leng Ying-Rong,Liu Ming-Ming et al. MicroRNA and long noncoding RNA involvement in gout and prospects for treatment.[J].Int Immunopharmacol, 2020, 87: 106842.doi:10.1016/j.intimp.2020.106842[2]Yu Yunfang,Zhang Wenda,Li Anlin et al. Association of Long Noncoding RNA Biomarkers With Clinical Immune Subtype and Prediction of Immunotherapy Response in Patients With Cancer.[J].JAMA Netw Open, 2020, 3: e202149.doi:10.1001/jamanetworkopen.2020.2149[3]Zou Yaoyao,Xu Siqi,Xiao Youjun et al. Long noncoding RNA LERFS negatively regulates rheumatoid synovial aggression and proliferation.[J].J Clin Invest, 2018, 128: 4510-4524.doi:10.1172/JCI97965Figure 1.Relative Expression of noncoding RNAs in the PBMCs of Patients.Disclosure of Interests:Quan-Bo Zhang Grant/research support from: the National Natural Science Foundation of China(General Program) (no.81974250) and Science and Technology Plan Project of Sichuan Province (no.2018JY0257), Yu-Qin Huang: None declared, Fan-Ni Xiao: None declared, gui-lin jian: None declared, Yi-Ping Tang: None declared, Fei Dai: None declared, Jian-Xiong Zheng: None declared, Yu-Feng Qing Grant/research support from: Science and Technology Project of Nanchong City (no.18SXHZ0522).

Background:CircRNAs have been found to be involved in the occurrence and development of many rheumatic diseases[1-2]. Are circRNAs involved in the pathogenesis of ankylosing spondylitis (AS)? How do these circRNAs participate in the pathogenesis of AS? This all needs further study.Objectives:This study is to clarify the expression levels of hsa_circ_0012732, hsa_circ_0008961, hsa_circ_0405239 and hsa_circ_0068784 in the peripheral blood of AS patients, and to explore whether these circRNAs are involved in the pathogenesis of AS.Methods:To collected 60 cases of AS (30 cases of active AS (ASA): BASDA> 6 or 6> BASDAI> 4, ESR> 22mm / h or 6> BASDAI> 4, hsCRP> 9mg / L; 30 cases of stable AS (ASS): BASDAI <4) and 30 health control (HC) peripheral blood samples, related clinical and laboratory indicators. The relative expression levels of hsa_circ_0012732, hsa_circ_0008961, hsa_circ_0405239 and hsa_circ_0068784 in each group were detected by real-time quantitative polymerase chain reaction (qPCR). The relationships between the 4 circRNAs and clinical and laboratory indicators were explored by correlation analysis.Results:1. The qPCR results suggested that the expression of hsa_circ_0012732 between the ASA and ASS groups was statistically significant (p<0.05), and the expression of hsa_circ_0008961 was statistically significant between the ASA and HC groups (p<0.05). Howeverthere was no statistical significance among other groups (p>0.05)Figure 1. Similarly, the expression level of hsa_circ_0405239 was not statistically significant among the groups (p>0.05), and the same was true for hsa_circ_0068784 (p>0.05).2. Correlation analysis results (Figure 2) showed that hsa_circ_0012732 is positively correlated with lymphocyte count (LY), mean corpusular volume (MCV), albumin (ALB), and negatively correlated with Bath ankylosing spondylitis disease activity index (BASDAI), Bath ankylosing spondylitis functional index (BASFI), high sensitivity C-reactive protein (hsCRP), Globulin (GLOB) (p<0.05); hsa_circ_0008961 is negatively correlated with platelet (PLT) (p<0.05); hsa_circ_0405239 is negatively correlated with BASDAI and BASFI; hsa_circ_0068784 was negatively correlated with BASDAI (p<0.05); and there was no statistically significant (p>0.05) between these 4 circRNAs and other indicators.Conclusion:Hsa_circ_0012732, hsa_circ_0008961, hsa_circ_0405239 and hsa_circ_0068784 may be related to the pathogenesis of AS. Among them, hsa_circ_0012732 may be involved in AS inflammation and has the potential to participate in the judgment of disease activity.References:[1]LS, K., et al., The biogenesis, biology and characterization of circular RNAs. 2019. 20(11): p. 675-691.[2]J, W., et al., Non-coding RNAs in Rheumatoid Arthritis: From Bench to Bedside. 2019. 10: p. 3129.Disclosure of Interests:Yi-Ping Tang Grant/research support from: Science and Technology Project of Nanchong City (18SXHZ0522)., Yu-Feng Qing: None declared, Zeng-Rong Dong: None declared, Fei Dai: None declared, Jianxiong Zheng: None declared, Yi Jiang: None declared, Yi-Xi He: None declared, Quan-Bo Zhang Grant/research support from: National Natural Science Foundation of China(General Program) (81974250); Science and Technology Plan Project of Sichuan Province (2018JY0257)

Abstract Objectives: Mesenchymal-type colorectal cancer (CRC), characterized by strong stromal infiltration and immune tolerance, resists immune checkpoint blockade and has poor outcomes. Cancer-associated fibroblasts (CAFs), abundant in tumor stroma, actively remodel the extracellular matrix (ECM), modulate immune evasion, and drive tumor progression. We have recently identified thioredoxin domain-containing protein 5 (TXNDC5), a protein disulfide isomerase (PDI), as a critical mediator of fibroblast activation and ECM remodeling in organ fibrosis. We hypothesized that TXNDC5 could contribute to fibroblast activation, stroma formation and disease progression in cancer, especially in the stroma-enriched fibrogenic mesenchymal-type CRC. Methods: Transcriptome databases of CRC were re-analyzed to determine the clinical relevance of TXNDC5. Experimentally, CRC was induced in mouse lines by azoxymethane (AOM) and dextran sulfate sodium (DSS) stimuli, a model sharing multiple characteristics with human mesenchymal-type CRC. Human colonic fibroblast line CCD-18co was used to investigate the molecular mechanisms by which TXNDC5 regulates colonic fibroblast activities. Fibroblast-specific TXNDC5 knockout (Col1a2-Cre/ERT2*TXNDC5fl/fl) mice were generated, combining with single-cell RNA sequencing analyses on AOM/DSS-induced CRC tumors in these animals, to clarify how fibroblast TXNDC5 impact tumor microenvironment, CRC progression and response to immune checkpoint blockade. Findings: TXNDC5 was predominantly expressed in stromal fibroblasts of human and mouse CRC. Fibroblast-specific deletion of Txndc5 lessened CAF activation, attenuated tumor fibrosis and reduced tumor burden in AOM/DSS-induced CRC. Mechanistically, increased TXNDC5 levels augments TGFβ signaling in CAF by post-translational stabilization of TGFBR1 through its PDI activity. In addition, deletion of Txndc5 in CAFs led to less tumor desmoplasia, decompressed tumor vessels and attenuated intra-tumoral hypoxia, thereby easing immune tolerance and increasing cytotoxic T cell infiltration in CRC. Single-cell transcriptome analysis revealed a marked change of intra-tumoral immune cell populations upon fibroblast-specific deletion of TXNDC5, shifting from myeloid-derived suppressive cells to cytotoxic tumor-infiltrating lymphocytes. Importantly, depletion of TXNDC5 in CAFs potentiated the anti-tumor effects of immune checkpoint blockade with anti-PD1 therapy in CRC. Conclusions: Our data suggest an important yet previously unrecognized role of fibroblast TXNDC5 in CRC progression, through enhancing CAF activation, stroma formation and immune escape. Combining immune checkpoint blockade with TXNDC5 deletion synergistically improved anti-tumor effects in CRC. Targeting TXNDC5, therefore, can be a novel therapeutic approach for CRC patients. Citation Format: Kai-Lin Cheng, Chih-I Chen, Shu-Han Yu, Huai-Wen Liang, Yi-Wei Tsai, Chen-Ting Hung, Yu-Shan Lin, Yi-Shiuan Tzeng, Sung-Jan Lin, Yueh-Feng Wu, Jen-Kuang Lee, Chia-Hui Yu, Shuei-Liong Lin, Shih-Yu Chen, Tzu-Tang Wei, Yun-Ju Huang, Ruey-Hwa Chen, Ching-Chow Chen, Kai-Chien Yang. Targeting TXNDC5 in stromal fibroblasts resolves desmoplasia and resistance to immune checkpoint blockade in mesenchymal-type colorectal cancer [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2024; Part 1 (Regular Abstracts); 2024 Apr 5-10; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2024;84(6_Suppl):Abstract nr 285.

Internal short circuit (ISC) is a critical failure mechanism for lithium-ion cells that can lead to disastrous thermal runaway. Therefore, great effort has been made to understand its mechanisms by purposefully triggering ISC through various methods1, 2, such as embedding a nickel particle3 or a wax covered copper pad4 inside lithium-ion cells. While these methods have been successful in triggering ISC, none of them allows measurement of ISC current, a critical parameter that influences heat generation and risk of thermal runaway. Alternatively, Feng et al.5 reported a method of placing a resistor between two parallel-connected cells and using the resistor to externally short circuit the cells. This method could allow measurement of short circuit current, but the resistor is placed between two cells rather than between electrode layers to create different types of ISC6. Inspired by this method5 and the established ISC methods3, 4, here we report a new method that not only triggers lithium-ion cell ISC but also monitors ISC current. As shown schematically in Figure 1, the method works by inserting a pair of small nickel pads inside a lithium-ion cell. One pad is between cathode and separator. The other is between anode and separator. Then the pads are connected electronically outside the cell through insulated metal wires, a switch and a current sensor. Once the switch is closed, the anode and cathode will be shorted at the pad region to form an Anode-Cathode type ISC. The ISC current is forced to flow through the external circuit to be measured by the current sensor. The resistance of the external circuit is much lower than the resistance between nickel pads and their corresponding electrode, so heat generation is focused inside the cell at the pad region as in an ISC scenario. By adjusting dimension of the nickel pads and/or the pressure applied to the pad region, the ISC resistance can be adjusted for investigation of its effects. Furthermore, by connecting one of the pads electronically to the opposite tab, the Anode-Aluminum type ISC and the Cathode-Copper type ISC, can be created. Alternatively, these types of ISC can be created by removing part of electrode coating and placing one of the Ni pads to be in direct contact with current collector. In addition, by embedding thermocouples inside the cell7, temperature distributions can be simultaneously measured for understanding of electrochemically-thermally coupled phenomena during ISC. Figure 2 shows preliminary results obtained from a prototype lithium-ion cell using this new method. The cell has a single unit of electrodes and a nominal capacity of 0.02 Ah. Three types of ISC were created, including Anode-Cathode, Anode-Aluminum and Cathode-Copper. As expected, the Anode-Cathode type ISC had the smallest current while the Anode-Aluminum type ISC had the highest current6, which can be attributed to the high resistance of cathode and the contact resistance between nickel pad and electrode. More results and analysis will be presented. References X. Lai, C. Jin, W. Yi, X. Han, X. Feng, Y. Zheng and M. Ouyang, Energy Storage Materials, 35, 470 (2021). G. Zhang, X. Wei, X. Tang, J. Zhu, S. Chen and H. Dai, Renewable and Sustainable Energy Reviews, 141, 110790 (2021). IEEE, IEEE Std 1625-2008 (Revision of IEEE Std 1625-2004), 1 (2008). E. Darcy and M. Keyser, On-Demand Cell Internal Short Circuit Device, in Power Sources Conference 2014; 9-12 Jun. 2014; , Orlando, FL; United States (2014). X. Feng, X. He, L. Lu and M. Ouyang, Journal of The Electrochemical Society, 165, A155 (2018). S. Santhanagopalan, P. Ramadass and J. Zhang, Journal of Power Sources, 194, 550 (2009). S. Huang, Z. Du, Q. Zhou, K. Snyder, S. Liu and G. Zhang, Journal of The Electrochemical Society, 168, 090510 (2021). Figure 1

The zinc/nickel electrochemical system has long been proposed as a good candidate of secondary alkaline batteries due to its excellent performance versus other aqueous batteries, such as high practical specific energy, excellent specific power, high open circuit voltage, low cost and low toxicity [1,2]. These advantages make it suitable for replacing lead-acid and nickel-cadmium batteries [3]. However, the high solubility of zinc in concentrated alkaline electrolytes is still a significant problem that induces two main failure mechanisms: a shape-change of the zinc electrode and a redistribution of the zinc active material due to its dissolution/redeposition during cycling. Dendritic growth can occur as a consequence of zinc dissolution/redeposition, and if severe, may lead to separators’ perforation and internal electrical short-circuits [4]. These drawbacks reduce the cell's capacity and lifetime, especially compared to traditional competing systems [5]. In addition, since the hydrogen evolution reaction (HER) is thermodynamically possible (especially during charging), the coulombic efficiency of the zinc electrode can be lowered by this parasite reaction [6]. The undesirable HER consumes water and some of the active material, yielding zinc hydroxide which in turn can generate a passivation layer that lowers the usability of the zinc anode materials [7]. There are different approaches to overcome these problems, such as the integration of additives in the active material formulation and/or in the electrolyte. In this contribution, we will show how regeneration of the active material can be obtained via appropriate steps of rest submitted to the active material and without the need for additional energy input. The so-called “self-healing” of the active material allows to recover a substantial part of the electrochemical performance. The concept was deeply studied and monitored by scanning electron microscopy coupled with elemental mapping by X-ray energy dispersive spectrometry, and operando tomography. An increase in the coulombic efficiency has been demonstrated making this discovery very promising for the future of zinc-based alkaline batteries. Keywords: Zinc-nickel batteries, additives, self-healing References: [1] M. Ma et al., “Electrochemical performance of ZnO nanoplates as anode materials for Ni/Zn secondary batteries,” J. Power Sources, vol. 179, no. 1, pp. 395–400, 2008, doi: 10.1016/j.jpowsour.2008.01.026. [2] S. H. Lee, C. W. Yi, and K. Kim, “Characteristics and electrochemical performance of the TiO 2-coated ZnO anode for Ni-Zn secondary batteries,” J. Phys. Chem. C, vol. 115, no. 5, pp. 2572–2577, 2011, doi: 10.1021/jp110308b. [3] B. Yang, Z. Yang, R. Wang, and Z. Feng, “Silver nanoparticle deposited layered double hydroxide nanosheets as a novel and high-performing anode material for enhanced Ni-Zn secondary batteries,” J. Mater. Chem. A, vol. 2, no. 3, pp. 785–791, 2014, doi: 10.1039/c3ta14237j. [4] Q. Zhang, J. Luan, Y. Tang, X. Ji, and H. Wang, “Interfacial Design of Dendrite-Free Zinc Anodes for Aqueous Zinc-Ion Batteries,” Angew. Chemie - Int. Ed., vol. 59, no. 32, pp. 13180–13191, 2020, doi: 10.1002/anie.202000162. [5] C. Chemist and B. Hill, “Introduction,” pp. 191–192, 1800. [6] S. Bin Lai et al., “A promising energy storage system: rechargeable Ni–Zn battery,” Rare Met., vol. 36, no. 5, pp. 381–396, 2017, doi: 10.1007/s12598-017-0905-x. [7] H. Kim, G. Jeong, Y. U. Kim, J. H. Kim, C. M. Park, and H. J. Sohn, “Metallic anodes for next generation secondary batteries,” Chem. Soc. Rev., vol. 42, no. 23, pp. 9011–9034, 2013, doi: 10.1039/c3cs60177c. Figure 1

Internal short circuit (ISC) can be a main cause of Li-ion cell thermal runaway in field failures, but its mechanisms still require better understanding1 , 2. Due to the highly localized and electrochemical-thermal coupled nature of ISC, it is important to use in situ/operando measurement of critical parameters for insightful understanding of the mechanisms. We recently reported a method for in situ measurement of dynamic ISC resistance and ISC current during nail penetration as schematically shown in Figure 1(a)3. When an ISC is formed inside the small test cell by nail penetration, external current will flow from the large power supply cell to the test cell. The test cell capacity is much smaller than the large power supply cell, so the measured current (A) can be assumed to be equal to the ISC current. The ISC voltage (V) is measured directly. Then the ISC resistance can be obtained through dividing the ISC voltage by the ISC current. The local temperature at the ISC spot is directly measured by a thermocouple embedded at the tip of the smart nail4. The previous method enabled insightful understanding of ISC3. It was observed that the ISC resistance changed by several orders of magnitude during nail penetration and dramatically influenced heat generation and local temperature rise. In some cases, the local ISC temperature increased more than 500 ℃, and even caused melting of Al foil in contact with the nail and rapid recovery of ISC resistance. But the previous method has a shortcoming. As noted in Figure 1(a), the small test cell with ISC is chemically and thermally disconnected from the large power supply cell. Such disconnection makes the thermal behaviors of the cells different from a real-world large Li-ion cell in which the ISC location is electrochemically and thermally connected to the entire cell. In particular, it does not allow investigation of thermal runaway propagation from the ISC location to the large cell. Built on our previous work while addressing its shortcoming, here we report an improved method. As shown schematically in Figure 1(b), a small cell and a large cell are fabricated inside the same pouch, sharing the same electrolyte and the same separator. They are not only electrically connected, but also chemically and thermally connected. The measurement of ISC current, resistance and temperature is similar to our previous work. When the smart nail penetrates the small cell, the ISC current flows from the large cell to the small cell through the external wire and can be measured by a current sensor (A). The ISC resistance is obtained from directly measured ISC current and ISC voltage. The local temperature can be measured not only by the thermocouple embedded at the tip of the smart nail, but also by thermocouples embedded at different locations inside the large cell5. This new method will enable insightful understanding of the highly localized and electrochemical-thermal coupled ISC phenomena under conditions closer to field failures. The experimental data can also be used for validation and improvement of numerical models of ISC. References X. Lai, C. Jin, W. Yi, X. Han, X. Feng, Y. Zheng and M. Ouyang, Energy Storage Materials, 35, 470 (2021). G. Zhang, X. Wei, X. Tang, J. Zhu, S. Chen and H. Dai, Renewable and Sustainable Energy Reviews, 141 (2021). S. Liu, S. Huang, Q. Zhou, K. Snyder, M. Long and G. Zhang, 241st ECS Meeting, May 29- June 2, 2022, Vancouver, BC, Canada (2022). S. Huang, X. Du, M. Richter, J. Ford, G. M. Cavalheiro, Z. Du, R. T. White and G. Zhang, Journal of The Electrochemical Society, 167 (2020). S. Huang, Z. Du, Q. Zhou, K. Snyder, S. Liu and G. Zhang, Journal of The Electrochemical Society, 168 (2021). Figure 1

Abstract Background- Ma recipe is an ancient Chinese medicine recipe used for venomous snake bites. Four Chinese herbal medicines, prunella, vitex seed, red grass and eucalyptus leaves, were deemed essential ingredients in the Ma recipe prescription. Since Ma recipe has a powerful analgesic effect, it has been used to treat serious cancer pain in our clinic for over decade. It has observed that patients with advanced lung cancer、advanced liver cancer and some of other solid tumor also survive significantly longer after the elimination of cancer pain. In this small number of retrospective case series, we report the efficacy of Ma recipe therapeutics and pathological changes of tumor lesion before and after treatment in patients with advanced esophageal cancer. Methods-Treatment was in the form of prescriptions written by traditional Chinese doctors. This behavior is consistent with the norms of traditional Chinese medicine, and does not deviate from the conventional clinical practice. Ma recipe should be taken orally every day at 10 grams. Benefits from Ma recipe therapeutics was assessed by comparing symptom improvement and pathological changes before and after treatment, as well as by survival analysis. Results- A total of 12 patients with advanced esophageal cancer were treated by Ma recipe therapeutics. Seven of 12 had severe obstructive symptoms of eating difficulty and weakness that it must be treated symptomatically. These symptoms were reduced or disappeared within 7 to 30 days of Ma recipe treatment and were sustained clinical recovery maintained thereafter. There were no events of death and disease progression occur in all the patients with Ma recipe intervention at the data cutoff point of the median duration of exposure to Ma recipe therapeutics was 48 months (range, 12-85 months). All the treated patients returned to normal life. Of the 12 cases, 9 had pathological examinations before and after administration of Ma recipe. Results all 9 patients were diagnosed esophageal cancer in the pathological biopsy tissue specimens obtained prior to administration of Ma recipe, but no cancer cells were found in all Pathological biopsy tissue specimens were obtained after Ma recipe treatment. Of the 9 cases undergoing pathological examination, 6 received radiotherapy after administration of Ma recipe. Ma recipetherapeutics has not been found to have serious side effects in years of clinical practice. Conclusions- The survival benefit of Ma recipe therapeutics for patients with advanced esophageal cancer is enormous. The ability of Ma recipe treatment to rapidly improve obstructive symptoms in advanced esophageal cancer is a highlight. The pathological changes before and after treatment support that Ma recipe therapeutics has a definite curative effect on esophageal cancer. However, this study is a post hoc secondary analysis and the limitation of the insufficient number must be treated with caution. This study can only give an indication that Ma recipe therapeutics has the potential to become an approach for patients with advanced esophageal cancer. Citation Format: Jiangnan Feng, Zhenghua Ma, Hua Wu, Meiying Gao, Chengsheng Yi, Xia LIU, Jiangnan Feng. The benefit of Ma recipe, A combination of Chinese herbal medicines for patients with advanced esophageal cancer: A retrospective case report series [abstract]. In: Proceedings of the AACR-NCI-EORTC Virtual International Conference on Molecular Targets and Cancer Therapeutics; 2023 Oct 11-15; Boston, MA. Philadelphia (PA): AACR; Mol Cancer Ther 2023;22(12 Suppl):Abstract nr C138.

Herbal extract, rich with natural compounds, has been used for medicinal purpose such as treating neurological disorders such as cognitive defection for a long period of time, often without significant adverse effects. We compared AChE and BuChE – inhibition effect of total extracts and fractions of Huperzia serrata (Thunb.) Trevis. with alcaloid-rich extract. Our samples were subjected under supersonic extraction with ethanol 50o as solvent and fractionally extracted with n-hexane, EtOAc and n-butanol, respectively; alcaloid-rich extract was collected simutaneously. Ellman’s method was used to assay AChE and BuChE inhibition activity. Results: Alcaloid-rich extraction proved to be the superior AChE inhibiting agent, its activity nearly 6 fold of the most active Huperzia serrata extraction with IC50 value of 7.93 (5.43-10.98) µg/ml. While the fractions as well as the total extract did not provide any BuChE inhibition activity, alcaloid-rich extract showed weak ability (IC50 at 76.67 (64.78 – 91.84) µg/ml). Overall, the superior enzyme inhibition effect of alcaloid-rich extract might open a new approach in preventing and treating neurological disorders such as alzheimer’s. Keywords Huperzia serrata (Thunb.) Trevis, alcaloid, Acetylcholinesrerase inhibitors (AChE); butyrylcholinesterase (BuChE), Alzheimer. References [1] Dos Santos Picanco, Leide C et al., Alzheimer's disease: A review from the pathophysiology to diagnosis, new perspectives for pharmacological treatment, Current medicinal chemistry 25(26) (2018) 3141 - 3159. https://doi.org/10.2174/0929867323666161213101126.[2] B.M. McGleenon, K.B. Dynan, A.P. Passmore, Acetylcholinesterase inhibitors in Alzheimer's disease, British journal of clinical pharmacology 48(4) (1999) 471-480. https://10.1046/j.1365-2125.1999.00026.x.[3] Agneta Nordberg, Clive Ballard, Roger Bullock, Taher Darreh-Shori, Monique Somogyi, A review of butyrylcholinesterase as a therapeutic target in the treatment of Alzheimer’s disease, The primary care companion for CNS disorders 15(2) (2013). https://10.4088/PCC.12r01412.[4] N.M. Ha, V.V. Dung et al., Report on the review of Vietnam’s wildlife trade policy, 2007.[5] D.H. Bich, et al., Medicinal plants and medicinal animals in Viet Nam. Science and Technics Publishing House 1 (2011) 896-897 (in Vietnamese).[6] Jia-Sen Liu, Yuan-Long Zhu, Chao-Mei Yu, You-Zuo Zhou, Yan-Yi Han, Feng-Wu Wu, Bao-Feng Qi, The structures of huperzine A and B, two new alkaloids exhibiting marked anticholinesterase activity. Canadian Journal of Chemistry 64(4) (1986) 837-839. https://doi.org/10.1139/v86-137.[7] Takuya Ohba, Yuta Yoshino et al., Japanese Huperzia serrata extract and the constituent, huperzine A, ameliorate the scopolamine-induced cognitive impairment in mice, Bioscience biotechnology and biochemistry 79(11) (2015) 1838-1844. https://doi.org/10.1080/09168451.2015.1052773.[8] Ju-Yeon Park, Hyuck Kim et al., Ethanol Extract of Lycopodium serratum Thunb. Attenuates Lipopolysaccharide-Induced C6 Glioma Cells Migration via Matrix Metalloproteinase-9 Expression, Chinese Journal of Integrative Medicine 24(11) (2018) 860-866. https://doi.org/10.1007/s11655-017-2923-9.[9] M. Maridass, G. 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碩士
國立臺北藝術大學
美術史研究所
99
Tang Yi-Fen painting results with his life experience and making friends in the object relationship is quite close, it can be said to be inseparable; his paintings selected from the intercourse of objects inspired by his painting turning point is that he traveled the Quartet after the sentiment. At that time, the background, also highlighted the particular style of painting in the Tang Yusheng: Ritual in the collapse, the country of the death of the era, he insisted the state''s desire for the literati on the screen to create the ideal of their own country, and rare is that in old age, he still tirelessly for the affairs of state. Paintings in the second part of Chapter Tang Yusheng, the author will be arranged after his work for stage. One of the paintings in the early part, due to the number of rare handed down, so can only be painted as supporting information recorded in the history of the medium-term paintings, better old age until maturity in the quality and quantity reached the peak. Painting studies, the third chapter, he will be middle of the Qing Da re-light "painting Quan" and his "painting Quan" subhead and make note of the "painting Tsuen analysis of access" in the cross-match that he''s Painting view, and his paintings and paintings on view integration; Finally, in the Tang Yusheng mainly on the impact for future generations to explore and positioning.