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Port, Andrew I. « Rethinking Regime Stability : The Life Stories of “Loyal” East German Activists in the Early German Democratic Republic ». Jahrbuch für Wirtschaftsgeschichte / Economic History Yearbook 58, no 2 (27 novembre 2017) : 367–412. http://dx.doi.org/10.1515/jbwg-2017-0014.
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Abstract In the early 1950s, East German officials at the storied Maxhütte steel mill in Thuringia collected short CVs or “life stories” (Lebensläufe) written by approximately 370 blue- and white-collar workers who had recently become Stakhanovite “activists”. These documents, which all contain the same basic biographical information about the authors – from their socioeconomic background to their political activities – shed light on a group that has received little systematic scholarly attention, namely, ostensibly loyal and ordinary East Germans at the grass roots. Their early support of the SED state and its economic goals ensured the longer-term stability of a largely unloved regime. These valuable documents thus provide important clues for understanding the puzzling political and economic viability of the German Democratic Republic.
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Giovani, Annalisa. « Le politiche per la grande viabilità nell'Italia della Prima repubblica : storia di una pianificazione mancata ». STORIA E PROBLEMI CONTEMPORANEI, no 77 (juillet 2018) : 61–79. http://dx.doi.org/10.3280/spc2018-077004.
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Jo, Sunggyung. « Homer’s Odyssey Once More : Magical Stories and Spellbound Audiences ». British and American Language and Literature Association of Korea 146 (30 septembre 2022) : 311–28. http://dx.doi.org/10.21297/ballak.2022.146.311.
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In this essay, I discuss specific ways in which the Odyssey ensures its reception by broader and future audiences by employing both professional and amateur storytellers and internal listeners as guarantors of the story's viability. My focus here is on the differences between Demodocus’s professional storytelling and Odysseus's nominally nonprofessional storytelling. While the former serves to please groups of an audience who expect “safe” pleasures by listening to traditional epic tales, the latter opens an affective space between listeners and the storyteller, where the listeners’ affects are mobilized by Odysseus’s unpredictable and perhaps less skillful ways of narrating stories. Odysseus’s amateurism (as I would call it) instigates the audience’s desire to listen more and becomes a successful strategy to captivate the audience’s minds. In this age and culture often defined by the “narrative turn,” many of us are interested in telling good stories that can impact broad audiences. This much-loved, ancient work of fiction can teach us various techniques of good storytelling that can move the hearts of generations of audiences.
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Zasada, John C., John C. Tappeiner II et Timothy A. Max. « Viability of Bigleaf Maple Seeds After Storage ». Western Journal of Applied Forestry 5, no 2 (1 avril 1990) : 52–55. http://dx.doi.org/10.1093/wjaf/5.2.52.
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Abstract Bigleaf maple is a common hardwood in western Oregon and Washington. Stored seeds from this species are generally believed to lose viability within a few weeks. In this study, two experiments were conducted with seeds collected in 1986 and 1987 in the Oregon Coast Range west of Corvallis, OR. Seeds collected in 1986 had a field moisture content (percentage of dry weight) of 49% and in 1987, 16%. The viability of the seed with the lowest moisture content declined from 75 to 60% during 1 year of storage at 1°C; the seed with the highest moisture content declined from about 90 to less than 10% viability. Storing the seeds at -10°C did not increase viability. Stratification of seeds for more than 60 days did not appear to increase germination. West. J. Appl. For. 5(2):52-55, April 1990.
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Bramlett, David L., et Frederick R. Matthews. « Storing Loblolly Pine Pollen ». Southern Journal of Applied Forestry 15, no 3 (1 août 1991) : 153–57. http://dx.doi.org/10.1093/sjaf/15.3.153.
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Abstract A series of controlled-pollination tests with fresh pollen and pollen stored for up to 10 years demonstrates the conditions required to maintain high pollen viability and vigor. During handling, freshly extracted pollen is highly resistant to exposure to temperatures up to 50°C for as long as 24 hours. Moisture content must be reduced to less than 10% prior to storage. It can then be held in a refrigerator at 3°C for up to 1 year in a container that maintains low moisture content. For longer storage, dried pollen should be placed in tightly closed glass or plastic bottles and held at -20°C. Pollen can be stored in vacuum-sealed ampules at 3°C for at least 10 years. South. J. Appl. For. 15(3):153-157.
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Carpenter, William J., et Eric R. Ostmark. « Moisture Content, Freezing, and Storage Conditions Influence Germination of Amaryllis Seed ». HortScience 23, no 6 (décembre 1988) : 1072–74. http://dx.doi.org/10.21273/hortsci.23.6.1072.
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Abstract Seeds of Hippeastrum × hybridum Hort. were subjected to dehydration, freezing, and storage for 12 months at various temperature and humidity levels. Amaryllis seed viability was unaffected by dehydration that removed 97% of available moisture content at seed harvest. Seeds were tolerant of freezing, but germination was reduced gradually as freezing temperatures decreased from 0° to −80°C. Moisture content at freezing influenced loss of viability; the lower the moisture content the higher the germination percentages. Temperature and relative humidity during seed storage governed seed viability loss rate. No measurable losses in viability resulted from storing seeds at 11% or 52% RH at 5° or 15°, respectively, for 12 months, but viability was significantly decreased when seeds were stored at these humidities at 25° or 35°. Viability decreased substantially or was lost after 3 months of storage at 75% and 95% RH and 25° and 35°.
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Ochieng, Olayo, et Lewis Kamau. « ANALYSIS OF THE VIABILITY OF LIFESTYLE AUDIT AS AN ANTI-CORRUPTION TOOL IN KENYA ». International Journal of Leadership and Governance 1, no 2 (8 décembre 2021) : 19–31. http://dx.doi.org/10.47604/ijlg.1426.
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Purpose: The main objective of this paper was to assess whether lifestyle audit as an anti-corruption tool is viable.
Methodology: The study was based on a desk review of existing studies and documented statistics. Further, legal framework and case laws were cited. A narrative analysis was performed and at this point the information was interpreted by comparing the results with the results of other empirical studies. This information was interpreted using the “stories in the stories” and linked to the existing literature.
Results: The study found that lifestyle audit is a viable tool for fighting corruption. However, there are challenges in the legal framework, particularly on the implementation. The study noted that the viability of lifestyle audits depends on judicial interpretation, political will and more importantly addressing of the current software and hardware issues existing in the current legal framework.
Recommendation: The study calls for political will to ensure the Lifestyle Audit Bill 2019 is not watered down by judicial interpretation. It further recommends that the political will should be accompanied by executive action to fight corruption through wealth declaration.
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Rubio, Francisco, et Carlos Llopis-Albert. « Viability of using wind turbines for electricity generation in electric vehicles ». Multidisciplinary Journal for Education, Social and Technological Sciences 6, no 1 (20 mai 2019) : 115. http://dx.doi.org/10.4995/muse.2019.11743.
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<p class="Textoindependiente21">This paper presents a feasibility study of applying a fluid energy recovery system by means of wind turbines for charging batteries of electric vehicles. This is because the main disadvantage of electric vehicles with regard to conventional fuel automobiles is the scarce capacity of storing sufficient energy to run long distances. This can be carried out by recovering a percentage of the energy used to overcome the aerodynamic drag of the vehicle. This work analysis different case studies, with different driving modes, to quantify the theoretical energy recovered from the vehicle aerodynamics. Results have shown the theoretical possibility to implement this technology in actual electric vehicles.</p>
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Souza, Patrícia Pereira de, Sérgio Yoshimitsu Motoike, Mychelle Carvalho, Kacilda Naomi Kuki, Eduardo Euclydes de Lima e. Borges et Antônio Marcos Silva. « Storage on the vigor and viability of macauba seeds from two provenances of Minas Gerais State ». Ciência Rural 46, no 11 (novembre 2016) : 1932–37. http://dx.doi.org/10.1590/0103-8478cr20150848.
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ABSTRACT: Macauba palm stands out for having favorable features to biodiesel production such as the high oil content of its fruit. Considering the great potential of the species and their applicability in the renewable energy field, it becomes indispensable to establish the right conditions for storing the seeds for propagation purpose. The aim of this research was to evaluate the effect of seed moisture content, packaging, and storage conditions such as temperature and relative humidity on the quality of seeds from Minas Gerais State, during a 12-month storage period. The research had two independent assays: (I) the seeds were stored with three moisture contents/ranges 4.0≤6.0%; 6.0≤8.0% and 8.0≤10.0% in impermeable packages, under room temperature and at 10ºC; (II) seeds with approximately 5.9% of moisture content were stored in three different types of packages: a) permeable, b) semi-permeable and c) impermeable. Three storing conditions were tested: a) room temperature and RH under laboratory conditions; b) 15ºC and 45% RH; c) 20ºC and 55% RH. Water content, germination rate and germination speed index were evaluated at 0, 4, 8 and 12 months of storing. The best germination results were obtained with the moisture range of 6.0≤8.0%, with seeds kept at room temperature; while the seeds stored at 10ºC, regardless the moisture range, did not survive. The stored seeds with 5.9% moisture content and at both 15ºC/45%RH and 20ºC/55% RH conditions, independently of the package type used, showed the best results. Thus, macaw palm seeds can be classified as intermediates seeds.
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McClanahan, Patrick D., Richard J. McCloskey, Melanie Ng Tung Hing, David M. Raizen et Christopher Fang-Yen. « Dehydrated Caenorhabditis elegans Stocks Are Resistant to Multiple Freeze-Thaw Cycles ». G3: ; Genes|Genomes|Genetics 10, no 12 (8 octobre 2020) : 4505–12. http://dx.doi.org/10.1534/g3.120.401825.
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Ultracold preservation is widely used for storage of genetic stocks of Caenorhabditis elegans. Current cryopreservation protocols are vulnerable to refrigeration failures, which can result in the loss of stock viability due to damage during re-freezing. Here we present a method for preserving worms in a dehydrated and frozen form that retains viability after multiple freeze-thaw cycles. After dehydration in the presence of trehalose or glycerol, C. elegans stocks can be frozen and thawed multiple times while maintaining viability. While both dauer and non-dauer larvae survive desiccation and freezing, the dauer defective mutant daf-16 does not survive desiccation. Our technique is useful for storing stocks in a manner robust to freezer failures, and potentially for shipping strains between laboratories.
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Lehra, Jonas, Evangelos Vrettos, Ram Rajagopal, Rishee Jain et Martin Everts. « Financial Viability of Residential Photovoltaic and Battery Systems in Californias ». Journal of Management and Sustainability 7, no 4 (25 octobre 2017) : 16. http://dx.doi.org/10.5539/jms.v7n4p16.
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Using a battery on a household level has become easier after the launch of Tesla’s Powerwall. Storing electricity during daytime’s PV overproduction or charging the battery during night with an attractive tariff is the most prominent applications. This paper explores the economic impact of the usage of residential battery storage combined with solar photovoltaics (PV) based on real load data from Northern California, USA. A data-driven, deterministic model to benchmark electricity cost savings for single households is presented and the financial viability of such systems is scrutinized for California. Our results indicate that under current capacity and price points, battery systems have limited financial viability and have a payback period exceeding 20 years in most cases. We deepen our analysis and compare the results of our deterministic model to that of a stochastic model to demonstrate that for an hourly time resolution the deterministic model provides an adequate benchmark for estimating cost (within 3%) savings with a short (1/60th) computation time.
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Pontes, Kelly Cristine de Sousa, Camila Almeida Ramalho, Andrea Pacheco Batista Borges, Rodrigo Viana Sepúlveda et Renato Barros Eleotério. « Microbiological viability of bovine amniotic membrane stored in glycerin 99% at room temperature for 48 months ». Revista Ceres 64, no 1 (février 2017) : 55–59. http://dx.doi.org/10.1590/0034-737x201764010008.
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ABSTRACT The medium for storing biological tissues is of great importance for their optimal use in surgery. Glycerin has been proven efficient for storing diverse tissues for prolonged time, but the preservation of the bovine amniotic membrane in glycerin 99% at room temperature has never been evaluated to be used safely in surgical procedures. This study evaluated the preservation of 80 bovine amniotic membrane samples stored in glycerin 99% at room temperature. The samples were randomly divided evenly into four groups. Samples were microbiologically tested after 1, 6, 12 and 48 months of storage. The presence of bacteria and fungi in the samples was evaluated by inoculation on blood agar and incubation at 37 ºC for 48 hours and on Sabouraud agar at 25 ºC for 5 to 10 days. No fungal or bacterial growth was detected in any of the samples. It was concluded that glycerin is an efficient medium, regarding microbiology, for preserving pre-prepared bovine amniotic membrane, keeping the tissue free of microorganisms that grow in the media up to 48 months at room temperature.
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Kovalevska, T. M., D. V. Krutylo, V. P. Horban, I. M. Romanova et T. O. Usmanova. « THE EFFECT OF STORAGE METHODS ON VIABILITY AND SYMBIOTIC PROPERTIES OF SOYBEAN NODULE BACTERIA ». Agriciltural microbiology 20 (22 décembre 2014) : 15–22. http://dx.doi.org/10.35868/1997-3004.20.15-22.
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The viability and symbiotic properties of active strain Bradyrhizobium japonicum 46 after its long-term storage using different methods were studied. It was established that strain storing in evaporative freezing conditions as well at periodic passages the strain preserves its ability to form active symbiosis with soybean plants. Long-term storage under the layer of mineral oil had negatively influenced on the symbiotic properties of B. japonicum 46 strain resulted in only partial restoration of strains virulence, nitrogen fixing activity and competitiveness.
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Danyuo, Y., C. J. Ani, J. D. Obayemi, S. Dozie-Nwachukwu, O. S. Odusanya, Y. Oni, Nicolas Anuku, Karen Malatesta et W. O. Soboyejo. « Prodigiosin Release from an Implantable Biomedical Device : Effect on Cell Viability ». Advanced Materials Research 1132 (décembre 2015) : 3–18. http://dx.doi.org/10.4028/www.scientific.net/amr.1132.3.
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This paper present the results of (experiments and models) biosynthesized prodigiosin (PG) released from an implantable biomedical device on the viability of cancer cells. The implantable biomedical devices were obtained from poly-di-methyl-siloxane (PDMS) packages with well-controlled micro-channels and drug storage compartments, along with a drug storing polymer core (which contains thermosensitive Poly (N-isopropylacrylamide)(PNIPA)-based gels). The results were compared with drugs elution from devices loaded with paclitaxelTM. The effects of localized release of PG and paclitaxel (PTx) on cell viability were elucidated via clonogenic assay testing on MDA-MB-231 breast cancer cell line. The effects of PG and PTx released were also tested over a range of temperatures (37-45 ̊C) in which localized hyperthermia is applicable. The trends in the results were analysed using statistical models before discussion their implications for localized treatment of breast cancer.
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Wahyuni, Ari, M. R. Suhartanto et Abdul Qadir. « Model Dinamik Vigor Daya Simpan Benih Kedelai pada Penyimpanan Terbuka ». Jurnal Penelitian Pertanian Tanaman Pangan 34, no 3 (10 février 2016) : 219. http://dx.doi.org/10.21082/jpptp.v34n3.2015.p219-227.
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<p>Soybean seed viability declines during seed storage. Soybean seed deteriorates rapidly, affected by its high protein content and often high humidity in the tropical environment. This research was aimed to develop dynamic model of soybean seed viability in an open storage. The study was conducted in three stages, namely: 1) desk study, 2) seed storage experiment, 3) development of seed storage model, simulation and verification of the model. The second stage of the experiment consisted of soybean seed storing and germination testing using completely randomized design. Treatments were three initial moisture content (7-8%, 9-10% and 11-12%) and four varieties of soybean (Anjasmoro, Wilis, Detam-1 and Detam-2). The results showed that the seed behaviour during storage period were affected by initial seed moisture content, initial viability, varieties and environmental condition. Therefore, seed moisture content, initial viability and varieties may be used as input model. Moisture content, integreting seed respiration, electric conductivity and seed storability vigor (VDSDB) were as model output. Simulation of Seed Storability Vigor Prediction Model with Model Construction Layer-Stella (MCLS) using relative humidity (RH), temperature, seed permeability, initial moisture content and initial viability as input model could logically predict the seed moisture content and seed storability vigor (VDSDB).</p>
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Bhattacharya, Amita, P. K. Nagar et P. S. Ahuja. « Seed development in Camellia sinensis (L.) O. Kuntze ». Seed Science Research 12, no 1 (mars 2002) : 39–46. http://dx.doi.org/10.1079/ssr200196.
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Seed development of tea was studied to identify the maturity index and the optimal time of seed collection. After harvest, the moisture content (28–30%fresh weight basis) of mature seeds, which germinated 100%, declined progressively (19% moisture content) after shedding, with a decrease in seed germination and viability. However, this viability loss could be prevented to some extent by storing seeds within intact fruits. The maximum rate of seed dry matter accumulation coincided with the accumulation of starch in the embryos and seeds at stage 8, the embryo maturation phase. Although the embryo abscisic acid (ABA) content was highest at stage 8, free ABA declined in the tea embryos throughout the remainder of the seed maturation cycle.
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Setyawardani, T., J. Sumarmono et D. R. Kusuma. « Microbiological profile of concentrated yoghurt manufactured from low and full fat milk during storage ». IOP Conference Series : Earth and Environmental Science 1041, no 1 (1 juin 2022) : 012077. http://dx.doi.org/10.1088/1755-1315/1041/1/012077.
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Abstract The microbiological profile illustrates the existence and growth of microflora in fermentation products, such as concentrate yogurt. The study aimed to identify and evaluate microflora in yogurt paste observed from the total bacteria, total lactic acid bacteria (LAB), total yeast, LAB viability, and level of acidity. Concentrate yogurt was made of full-fat milk and low-fat milk that was fermented and hung for 24h and stored at a low temperature for observation on day 0,15, 30, 45, and 60. The data were subjected to a Generalized Linear Model (GLM) followed by the Duncan test. The result showed significant (P<0.05) changes in the total bacteria, total LAB, total yeast, LAB viability dan acidity (pH) of the fermentation products; however, no significant difference (P>0.05) was identified regarding the use of raw material. Storage made the fermented products suffer from the decreased total bacteria, LAB dan its viability, but there was an increase in the total yeast and pH. Conclusively, storing concentrate yogurt in a cool place for 60 would decrease the microflora profile (total bacteria, LAB dan LAB viability) but increase the total yeast and pH value.
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Andrén, Anders H. G., et Anders P. Wieslander. « Survival and Function of Fibroblasts Stored as Stock Cultures at a Non-freezing Temperature ». Alternatives to Laboratory Animals 21, no 2 (avril 1993) : 206–9. http://dx.doi.org/10.1177/026119299302100215.
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Cytotoxicity, measured as inhibition of cell growth of cultured cell lines, is a widely used method for testing the safety of biomaterials and chemicals. One major technical disadvantage with this method is the continuous routine maintenance of the cell lines. We decided to investigate the possibility of storing stock cultures of fibroblasts (L-929) in an ordinary refrigerator as a means of reducing the routine workload. Stock cultures of the mouse fibroblast cell line L-929 were prepared in plastic vials with Eagle's minimum essential medium. The vials were stored in a refrigerator at 4–10°C for periods of 7–31 days. The condition of the cells after storage was determined as cell viability, cell growth and the toxic response to acrylamide, measured as cell growth inhibition. We found that the L-929 cell line can be stored for 2–3, weeks with a viabilty > 90% and a cell growth of about 95%, compared to L-929 cells grown and subcultured in the normal manner. The results also show that the toxic response to acrylamide, using refrigerator stored L-929 cells, corresponds to that of control L-929 cells. We concluded that it is possible to store L-929 cells in a refrigerator for periods of up to 3 weeks and still use the cells for in vitro cytotoxic assays.
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Noya, Marselina, Johan Riry et Marthini Lesilolo. « Pengaruh Media Dan Periode Simpan Terhadap Viabilitas Benih Cengkeh Tuni (Syzygium aromaticum L.) ». JURNAL BUDIDAYA PERTANIAN 14, no 2 (1 décembre 2018) : 97–104. http://dx.doi.org/10.30598/jbdp.2018.14.2.97.
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This study aims to examine the influence of the media and the storage period on the viability of clove seeds. The study was conducted at the Ambon Plant Crops Protection and Protection Center laboratory. The study used a factorial completely randomized design with two types of factors and three replications. The storage media consists of 3 levels, namely: Without the storage media, the media for storing rice husk and the media for storing sawdust. The second factor is the storage period consists of 4 levels, namely: 10 days storage, 20 days storage, 30 days storage and 40 days storage. The parameters measured were germination power, germination rate, and vigor index. Storage media and storage period significantly affected germination and vigor indexes but did not significantly affect germination rates. The results showed that the sawdust storage media was a good medium for storing clove seeds (Syzygium aromaticum L.). This can be seen in the results of the average germination of clove seeds stored in powder storage media with a storage time of 10 days giving better results of 85% compared to rice husks at a storage time of 10 days with a germination percentage of 65%.
Keywords: clove seeds, save media, save period
ABSTRAK
Penelitian ini bertujuan untuk menguji pengaruh media dan periode simpan terhadap viabilitas benih cengkeh. Penelitian dilaksanakan di laboratorium Balai Besar Perbenihan dan Proteksi Tanaman Perkebunan Ambon. Penelitian menggunakan rancangan acak lengkap pola faktorial dengan dua macam faktor dan tiga kali ulangan. Media simpan terdiri dari 3 taraf yaitu: Tanpa media simpan, media simpan sekam padi dan media simpan serbuk gergaji. Faktor kedua adalah periode simpan terdiri dari 4 taraf yaitu: penyimpanan 10 hari, penyimpanan 20 hari, penyimpanan 30 hari dan penyimpanan 40 hari. Parameter yang diukur adalah daya berkecambah, laju perkecambahan dan indeks vigor. Media simpan dan periode penyimpanan berpengaruh nyata pada persentase perkecambahan dan indeks vigor tetapi tidak berpengaruh nyata pada laju perkecambahan. Hasil penelitian menunjukan bahwa media simpan serbuk gergaji merupakan media yang baik bagi penyimpanan benih cengkeh (Syzygium aromaticum L.). Hal ini terlihat pada hasil rata-rata daya berkecambah benih cengkeh yang disimpan pada media simpan serbuk dengan lama penyimpanan selama 10 hari memberikan hasil yang lebih baik yakni 85% jika dibandingkan dengan sekam padi pada lama penyimpanan 10 hari dengan persentase perkecambahan 65%.
Kata Kunci: benih cengkeh, media simpan, periode simpan
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Kuksa, Tatiana L. « “THE HUSBAND RETURNS FROM A BUSINESS TRIP” : DISCIPLINARY VIEWS ON ADULTERY IN AN ANECDOTE ». Practices & ; Interpretations : A Journal of Philology, Teaching and Cultural Studies 7, no 2 (1 juin 2022) : 162–82. http://dx.doi.org/10.18522/2415-8852-2022-2-162-182.
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This research proposes sociological, linguistic, folklore (psychoanalytic and structuralsemiotic) approaches to the analysis of the contemporary anecdote about adultery. Academic interpretations of satirical texts are quite different, but they allow us to explain the popularity and cultural reproduction of anecdotes about married life, gender stereotypes, the reasons for the women demonizing and men derision, as well as the modern specifics of a joke and recreation of its laughing effect. However, the viability of such stories about female infidelity is connected not only with the communicative situation. The article also analyzes the unified structure of the plot, developing around a trick, and the connection between medieval fabulous stories about “women’s tricks” and modern jokes with a typical beginning “the husband returns from a business trip”. Such anecdotes often use the same plots with a trick, motifs and formulaic beginning, as well as anecdotal stories about women’s cheating. In a contemporary joke, there is no explicitly pronounced didactics and morals, but it has some similarities with fabulous stories – exposure of its fictional status, formulaic beginning, plotting caused by tricks and savvy of characters, comic denouement and laughing effect.
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Carvalho, Everson Reis, João Almir Oliveira, Denilson Paulo da Rosa Mavaieie, Hellismar Wakson da Silva et Cassiano Gabriel Moreira Lopes. « Pre-packing cooling and types of packages in maintaining physiological quality of soybean seeds during storage ». Journal of Seed Science 38, no 2 (juin 2016) : 129–39. http://dx.doi.org/10.1590/2317-1545v38n2158956.
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Abstract: Seeds storing under appropriate conditions reduces the speed of the seeds deterioration process. The aim of this study was to evaluate pre-packing cooling and types of packages in the maintenance of the physiological quality of soybean seeds during storage. The completely randomized experimental design was used, with four replications, considering a factorial arrangement involving or not the pre-packing cooling of the seed mass, three types of packages (multi-layer paper bag, big bag, and polyethylene container), and five storing periods (0, 2, 4, 6 and 8 months). The physiological quality was evaluated by the tests of first germination count, germination, accelerated aging, electrical conductivity, emergence speed index and emergence. The use of packages with larger amount of seeds (big bag and polyethylene container) did not favor the maintenance of the pre-packing cooling temperature. The types of packages did not show significant differences in the seeds viability during storage, up to eight months, regardless the pre-packing cooling. The cultivars seeds showed different tolerance to storing, i.e. seeds of the cultivar TMG 1176 had lower storing potential comparing to the seeds from the cultivar SYN 9074.
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Korcok, Davor, Nada Trsic-Milanovic, Marija Ilic, Bogdan Mitic, Brizita Djordjevic et Nevena Ivanovic. « Improving the viability and stability of a probiotic product with Saccharomyces boulardii DBVPG ». Chemical Industry 75, no 1 (2021) : 25–30. http://dx.doi.org/10.2298/hemind201211008k.
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In recent decades, probiotic products have been increasingly used to prevent certain gastro-intestinal and urogenital disorders, to improve the general condition of the body and as a supplement to pharmacological therapy. They are most often registered as dietary supplements, and less often as drugs in the form of capsules, powders, and solutions. Optimization of technological processes of production and packaging of these products aims to maintain probiotic characteristics while adhering to all criteria during production that ensure quality, bioavailability and optimal therapeutic effects. In this paper, the importance of choosing primary and secondary packaging materials was explored with the aim of preserving the viability of probiotic cells in capsules for two years, i.e. during the shelf life. By comparing the applications of polyvinyl chloride (PVC) and PVC/polyvinylidene chloride/polyethylene blister foils, better protection of probiotic cells was observed by applying the multilayer foil. In addition, in this research, further improvements of probiotic cell protection were achieved by applying a secondary packing-flow pack bag with inert gas for storing multilayered blisters.
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Damayanti, Frisca, et Fitri Fatma Wardani. « Shelf Life of Artocarpus lowii King’s Seeds And Its Viability ». KnE Life Sciences 3, no 4 (27 mars 2017) : 94. http://dx.doi.org/10.18502/kls.v3i4.692.
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<p class="Els-Abstract-text"><em>Artocarpus lowii</em> King is a species of Moraceae family members. This species is widely utilized by people as food, medicine, and timber. <em>A. lowii</em> is propagated by seed. <em>A. lowii</em> seeds belong to the recalcitrant seeds, which have a short shelf life. Therefore, it is necessary to find the right storage method for storing <em>A. lowii</em> seeds, in order that <em>A. lowii</em> seed has longer shelf life. The purpose of this study was to determine the viability <em>A. lowii</em> with storage time treatments and find the efficient storage method. <em>A. lowii</em> seeds were stored by covering the seeds with mosses in a jar. It was taken 40 seeds every week for viability test. The 30 seeds have planted in the sand media and 10 seeds for grain moisture content calculations. Results of this study were water content of <em>A. lowii</em> seed on 0 wk to 7 wk increased, and the viability of <em>A. lowii</em> seeds have still high enough to 9 wk around 60 %. Therefore, seed storage methods using mosses can be used as an alternative to extend the shelf life of <em>A. lowii</em> seed.</p><p> </p><div><p class="Els-keywords"><strong>Keywords:</strong><em> Artocarpus lowii</em> King; recalcitrant seed; storage media</p></div>
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Bintara, S., I. Ismaya, D. T. Widayati, R. N. Aji et W. Asmarawati. « The effect of vitamin e antioxidant addition in goat milk diluent on the quality of thin-tailed sheep semen ». IOP Conference Series : Earth and Environmental Science 1001, no 1 (1 mars 2022) : 012012. http://dx.doi.org/10.1088/1755-1315/1001/1/012012.
Texte intégralRésumé :
Abstract Artificial insemination supports the prevention of infectious dispersion in organisms which share the same environment. Meanwhile, spermatozoa usually experience a decrease in quality during processing due to the peroxidation process. This study aims to identify the effect of vitamin E antioxidant addition in goat milk diluent on the quality of thin-tailed sheep semen after storing at 5°C for five days. A total of 4 male thin-tailed sheep were used and the treatments consisted of controls, namely goat milk sperm diluent without vitamin E antioxidant (P0), and others with the addition of vitamin E at a dose of 2 gL-1 diluent (P1); 3 gL-1diluent (P2); and 4 gL-1 (P3). Moreover, the variables observed include individual motility, viability, and intact plasma membrane of spermatozoa after storing at 5°C for five days. The data were analyzed statistically using analysis of variance (ANOVA) with a completely randomized design in one-way. The results showed that the addition of vitamin E antioxidant to goat milk diluent during sperm dilution had a significant effect (P < 0.05) on the motility, viability, and intact plasma membrane. Spermatozoa motility at P0, P1, P2, and P3 were 44.0%, 48.2%, 50.4%, and 53.2%, while the viability was 48.2%, 51.4%, 54.2%, and 56.0%, respectively. Additionally, the intact plasma membranes at P0, P1, P2, and P3 were 41.0%, 46.6%, 48.2%, and 51.4%, respectively. The addition of antioxidant vitamins in goat milk diluent with a concentration of 4 gL-1 produced the best results on sperm quality after cooling. Based on the results, it was concluded that the addition of vitamin E antioxidant to the sperm diluent can improve the quality of thin-tailed sheep chilled semen.
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Saakian, Alexander. « Effect of freezing seeds of kenaf (Hibiscus cannabinus L.) on germination, growth and development of plants ». АгроЭкоИнфо 4, no 46 (31 août 2021) : 25. http://dx.doi.org/10.51419/20214425.
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Abstract A comparative study of the effect of different storage regimes of seeds (at + 5 ° С, -20 ° С and under deep freezing conditions at -196 ° С) of a valuable bast fiber culture - kenaf - Hibiscus cannabinus L. on their viability and the development of plants from them in field conditions. Freezing increases the laboratory and field germination of kenaf seeds, and also increases the energy of their germination. It did not cause the appearance of non-viable, ugly seedlings and plants, as well as their death. Plants went through a full development cycle and set full-fledged seeds. Biometric parameters of plants obtained from thawed seeds and control ones are very close. Cryopreservation is a promising way of storing seeds of the studied species, since it ensures longer periods of preservation of their viability. Keywords: KENAF, SEED BANKS, LONG-TERM SEED STORAGE REGIMES, CRYOCONSERVATION
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Suma, A., Kalyani Sreenivasan, A. K. Singh et J. Radhamani. « Role of Relative Humidity in Processing and Storage of Seeds and Assessment of Variability in Storage Behaviour inBrassicaspp. andEruca sativa ». Scientific World Journal 2013 (2013) : 1–9. http://dx.doi.org/10.1155/2013/504141.
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The role of relative humidity (RH) while processing and storing seeds ofBrassicaspp. andEruca sativawas investigated by creating different levels of relative humidity, namely, 75%, 50%, 32%, and 11% using different saturated salt solutions and 1% RH using concentrated sulphuric acid. The variability in seed storage behaviour of different species ofBrassicawas also evaluated. The samples were stored at40±2°Cin sealed containers and various physiological parameters were assessed at different intervals up to three months. The seed viability and seedling vigour parameters were considerably reduced in all accessions at high relative humidity irrespective of the species. Storage at intermediate relative humidities caused minimal decline in viability. All the accessions performed better at relative humidity level of 32% maintaining seed moisture content of 3%. On analyzing the variability in storage behaviour,B. rapaandB. junceawere better performers thanB. napusandEruca sativa.
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Lloyd, R. E., R. M. A. Elliott, A. Fazeli, P. F. Watson et W. V. Holt. « Effects of oviductal proteins, including heat shock 70 kDa protein 8, on survival of ram spermatozoa over 48 h in vitro ». Reproduction, Fertility and Development 21, no 3 (2009) : 408. http://dx.doi.org/10.1071/rd08204.
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Following insemination, ram spermatozoa are transported to the isthmus region of the oviduct where they bind to the oviductal epithelial cells (OEC), remaining viable for several hours. The aim of the present study was to begin to decipher which component(s) of the ewe oviduct actively participates in maintaining the viability of ram spermatozoa. A series of experiments was conducted to investigate whether: (1) soluble OEC apical plasma membrane proteins (sAPM) isolated from ewes prolong survival of ram spermatozoa over an extended (48 h) coincubation period at 39°C; (2) a recombinant form of one of these oviductal proteins, namely heat shock 70 kDa protein 8 (HSPA8), prolongs survival of ram spermatozoa; and (3) pretreatment with HSPA8 antibody compromises the ability of sAPM to prolong the survival of ram spermatozoa. Both sAPM and recombinant HSPA8 had a beneficial effect on the viability of ram spermatozoa during coincubation, although both these effects were dose dependent. In contrast, pretreatment with HSPA8 antibody significantly negated the ability of sAPM to maintain the viability of ram spermatozoa. These findings suggest that HSPA8 is an active component of the ewe oviduct that participates in maintaining the viability of ram spermatozoa. This is a potentially valuable observation given that there is a great deal of room for improving existing diluents for storing fresh ram semen.
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Tarabukina, N. P., S. G. Petrova et M. P. Neustroev. « METHOD OF STORING THE SALMONELLA ABORTUS EQUI BACTERIA «BN-12» ON THE ZEOLITE «HONGURIN» ». Problems of Veterinary Sanitation, Hygiene and Ecology 1, no 3 (2018) : 61–64. http://dx.doi.org/10.36871/vet.san.hyg.ecol.201803011.
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The paper presents the results of studies on the testing of zeolite-hongurin for the preservation of cultures of the strain of bacteria Salmonella abortus equi "BN-12" in the conditions of the chamber (+4°C) and the freezer (-18°C) of the refrigerator. It was found that zeolite-hongurin can be used in the storage of microorganisms, since the bacteria Salmonella abortus equi "BN-12" adsorbed on the zeolite-hongurin during storage at temperature of 4° C to -18° C (respectively, in the freezing and refrigerating conditions chambers of the refrigerator), retain viability and pathogenicity for more than 12 months (observation period).
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Nosrati, Fariba, Claudia Crippa et Brian Detlor. « Connecting people with city cultural heritage through proximity-based digital storytelling ». Journal of Librarianship and Information Science 50, no 3 (7 mai 2018) : 264–74. http://dx.doi.org/10.1177/0961000618769972.
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This paper describes a research investigation on a project led by two libraries, Hamilton Public Library and McMaster University Library, in Hamilton, Canada, concerning the use of proximity-based technologies to share digital stories about a city’s culture. Proximity-based technology systems, such as iBeacons, allow users to receive information automatically when they are close to a physical spot. The project involved the setup of iBeacons that disseminated digital stories pertaining to Gore Park – a prominent historical park in the heart of downtown Hamilton. To test the viability of using iBeacon technologies to raise interest in a city and promote appreciation for a city’s cultural heritage, a pilot study was conducted. The study included one-on-one interviews and a short survey with 50 participants from the general public immediately after these participants used an iBeacon app to experience digital stories about Gore Park. Findings suggest iBeacons are viable tools to share city cultural heritage stories that yield improved perceptions of a city and greater appreciation for a city’s culture and history. Participants were appreciative of the digital stories and the iBeacon app. All participants mentioned that they learned something new about the city and that the app was very informative. Findings indicate that individual differences are important and can affect not only the acceptance and use of an iBeacon digital storytelling app, but also the extent to which the app can promote interest in a city and appreciation for a city’s cultural heritage.
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MASHUD, N., et DAVID ALLORERUNG. « PENGARUH PENYIMPANAN TERHADAP SIFAT KIMIA DAN VIABILITAS BENIH AREN ». Jurnal Penelitian Tanaman Industri 7, no 4 (15 juillet 2020) : 108. http://dx.doi.org/10.21082/jlittri.v7n4.2001.108-112.
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<p><strong>Effect of storage duration on physical and chemical characteristics and viability of seed in sugar palm Arenga pinnata</strong></p><p>Effect of storage on (he physical and chemical characteristics and viability of sugar palm seed was studied in (he Research Institute for Coconut and Other Palm, Manado from April 1997 lo March 1998. The seeds were collecled from (he palm of 15 year old from Kemelcmbuai village, Motoling sub district, Minahasa North Sulawesi. The seeds were sown in the glass house. The carbohydrate, lipid and protein were analyzed at the laboratory of Research Institute for Coconut and Other Palm, Manado. The experimcnl was designed as a randomized block arranged factorial ly. The irst factor was storage duration (0,2, 4, 6, 8, or 10 weeks). Results showed that unshclling affected germination rate but did not germinability. The seed stored for 2 - 4 weeks showed high gcrminability (71%). Unshclling and storing reduced carbohydrate and lipid, but not protein. Carbohydrate reduction occurred at two weeks storage, either in (he shelled or unshellcd seed. Reduclion of carbohydrate more rapidly in (he shelled seed stored for 2-4 weeks. Storage for 2 weeks resulted in the reduction of protein. From this study it was suggested that for high germination, Ihe seed should be stored for 4 weeks before sowing.</p>
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Sitepu, Sukma Aditya, Zaituni Udin, Jaswandi Jaswandi et Hendri Hendri. « QUALITY DIFFERENCES OF BOER LIQUID SEMEN DURING STORAGE WITH ADDITION SWEETORANGEESSENTIAL OIL IN TRIS YOLK AND GENTAMICIN EXTENDER ». Journal of Community Research and Service 1, no 2 (29 mars 2018) : 78. http://dx.doi.org/10.24114/jcrs.v1i2.9341.
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AbstractThe purpose of this study was to know the quality of Boer liquid semen during storage by adding sweet orange essential oil and gentamicin into the tris yolkextender.The semenlongevity test was carried out by storing semen in a closed tube at room temperature and refrigerator, and evaluated motility and Viability every 3 hours at room temperature and 12 hours in refrigerator. The results showed that the characteristics of the liquid semen, with the addition of 1% of essential oils showed percentage of motility and Viability significantly higher than 0.5% and without the addition of essential oil, either stored at room temperature or in the refrigerator. This may be related to the content of essential oils of sweet orange peel containing flavonoids and antibacterials that are capable of maintaining liquid semen quality of Boer Goat.In addition, gentamicin contains antibacterials capable of suppressing the growth of bacteria which can damage and durabilitythe spermatozoa.Keywords: Boer Goat,Gentamicin, Liquid semen, Sweet orange
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Van der Pas, Daphne Joanna, et Loes Aaldering. « Gender Differences in Political Media Coverage : A Meta-Analysis ». Journal of Communication 70, no 1 (février 2020) : 114–43. http://dx.doi.org/10.1093/joc/jqz046.
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Abstract Do the media cover men and women politicians and candidates differently? This article performs a systematic analysis of 90 studies covering over 25,000 politicians in over 750,000 media stories, and presents the accumulated knowledge in a comprehensive theoretical framework. The paper shows that there is a gender bias in the amount of coverage of politicians in proportional electoral systems, where women politicians lag behind men in media attention, but that, surprisingly, this gender bias is absent in majoritarian electoral systems. In addition, we systematically review gender differences in the content of media reports on political candidates, such as differences in attention to private life and family, viability and horse-race coverage, issue coverage, and gender stereotypes. Overall, women politicians receive more attention to their appearance and personal life, more negative viability coverage, and, to some extent, stereotypical issue and trait coverage. We conclude by pointing out promising avenues for future research.
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Suita, E., Nurhasybi, D. Syamsuwida, A. Aminah et K. P. Putri. « Seed storability of malapari (Pongamia pinnata Merril) ». IOP Conference Series : Earth and Environmental Science 1133, no 1 (1 janvier 2023) : 012056. http://dx.doi.org/10.1088/1755-1315/1133/1/012056.
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Abstract Seed storage is carried out to secure the seed stock obtained from the harvest until they are planted. The appropriate techniques were used to maintain the seed viability during storage. The objective of the research is to evaluate the viability of malapari (Pongamia pinnata) seeds after being treated. The seeds are stored in a room in sealed plastic with no media, sealed plastic + coconut coir powder media, sealed plastic + husk charcoal. The other containers were calico fabric with no media, calico fabric + coconut coir powder media, and calico fabric + husk charcoal. The finding showed that the best storage was storing the seed in a plastic container plus husk charcoal media, and within five weeks, it can maintain the seed moisture content of 62.92%, germination percentage of 93.33%, and germination speed of 7.55% KN/et mal. The seed can also be stored in a plastic container without media at a moisture content of 60.69%, and a germination percentage of 91.00%.
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Silva, Lucas Vilela Perroni, Duvaldo Eurides, Marina Greco Magalhães Guerra de Andrade, Marcelo Carrijo da Costa, Vinicius Candido Ferreira, Marina De Oliveira Silva et Ednaldo Carvalho Guimarães. « Conservation and viability of refrigerated platelet-rich plasma from New Zealand rabbits ». Semina : Ciências Agrárias 37, no 4 (30 août 2016) : 1981. http://dx.doi.org/10.5433/1679-0359.2016v37n4p1981.
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Fifteen male New Zealand rabbits were used in this study, with the aim of storing their platelet-rich plasma (PRP) for 30 days at 4-6 °C to investigate its conservation and viability during this period. Thirty samples of PRP were prepared and sorted into three equal groups (G1, G2, and G3), and every three days a sample was taken out for evaluationof the number of platelets, mean platelet volume (MPV), pH of the plasma, aggregation post addition of calcium thromboplastin, and for the presence of bacterial and fungal contamination. Results suggested that, for the number of platelets, there was no linear relationship over time. However, when comparing the number of platelets pre-storage to that post-storage, a statistical difference was observed. The hemogram MPV variables, pre and post-storage, also did not relate with time however, there was a statistical difference between the MPV of hemogram and MPV pre-storage, and between MPV pre-storage and MPV post-storage. From the pH evaluation, no influence of time on the variables was found, but statistical differences were found in the samples after storage between 30 and 6 days, 30 and 24 days, and 30 and 27 days. Platelet aggregation occurred within twenty seconds in all samples, independent of storage time. There was no growth of bacteria or yeast in any sample; however, mold growth occurred in the samples stored for 21 days from G1 and G3. It can be concluded that the PRP of rabbits can be stored in 4-6 °C refrigeration for up to18 days and still maintain the number of platelets, with no significant pH alteration or bacterial or fungal contamination.
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Rowan, Mary Caroline. « Rethinking relationships with curriculum by engaging with foxes and sharing stories in early childhood spaces ». Global Studies of Childhood 7, no 2 (juin 2017) : 131–47. http://dx.doi.org/10.1177/2043610617703833.
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Nunangat pedagogies concern the adoption of teaching practices informed by relationships with land, water and ice. In this article, the researcher examines an opportunity to disrupt Global North dominance in the Inuit homeland through engagements with fox. Nunangat methodologies require consultations with Elders and hunters especially concerning knowledge that is not accessible via the Internet or at the library. A rhizomatic analysis is used to connect the presentation of the various research narratives and analysis of encounters with fox. These strategies are employed to facilitate occasions to re-conceptualize early childhood practices in ways which enable recognition of the vitality and viability of local Indigenous ways of knowing and being.
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ADEKUNLE, A. O., A. C. S. PORTO-FETT, J. E. CALL, B. SHOYER, K. GARTNER, L. TUFFT et J. B. LUCHANSKY. « Effect of Storage and Subsequent Reheating on Viability of Listeria monocytogenes on Pork Scrapple† ». Journal of Food Protection 72, no 12 (1 décembre 2009) : 2530–37. http://dx.doi.org/10.4315/0362-028x-72.12.2530.
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We evaluated the fate of Listeria monocytogenes on commercial pork scrapple, a regionally popular, ready-to-eat (RTE) meat. We also conducted an informal survey to address consumer practices for storing and reheating scrapple. Of the 129 consumers who responded to at least one of the eight questions posed in the survey, about half (46.4%; 52 of 112) considered scrapple RTE, the majority (69.7%; 76 of 109) stored it in the refrigerator, and all (100%; 112 of 112) preferred to reheat it prior to consumption. Most respondents (83.9%; 94 of 112) reheated the scrapple by pan frying for 1 to 10 min at medium to high temperature. To study pathogen behavior, slices of pork scrapple were surface inoculated with a five-strain cocktail of L. monocytogenes (ca. 2.0 log CFU/g), vacuum sealed, and stored for up to 60 days. Pathogen levels increased to 8.9, 9.5, and 9.9 log CFU/g after 44 (4°C), 21 (10°C), and 5 (21°C) days, respectively. When slices 1.3 cm (ca. 55 g) and 1.9 cm (ca. 85 g) thick were surface inoculated with L. monocytogenes (ca. 7.0 log CFU/g) and then reheated in a skillet (191°C) for 0.5 to 4 min per side or to target instantaneous internal temperatures of 48.9 to 71.1°C, it was possible to achieve pathogen reductions ranging from ca.
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Rahman, Abdul, Syahbudin Hasibuan, Dwika Karima Wardani et Agus Ardi. « Effect of Media Type and Storage Duration on Viability of Cocoa (Theobroma cacao L.) Seeds ». International Journal of Engineering, Science and Information Technology 2, no 3 (28 juillet 2022) : 15–20. http://dx.doi.org/10.52088/ijesty.v2i3.263.
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This study aims to determine the best storage media and storage time for cocoa seeds. The research was carried out at the Plant Physiology Laboratory, Faculty of Agriculture, Medan Area University. The analytical method used is Completely Randomized Design (CRD) with treatments, namely No Storage Media, Use of PE Plastic Storage Media + 1 Week Storage, PE Plastic + 2 Weeks Storage, PE Plastic + 3 Weeks Storage, Aluminum Foil Paper + 1 Week Storage, Aluminum Foil + 2 Weeks Storage, Aluminum Foil Paper + 3 Weeks Storage, Burlap Sack + 1 Week Storage, Burlap Sack + 2 Weeks Storage, Burlap Sack + 3 Weeks Storage, Glass Bottle + 1 Week Storage, Glass Bottle + 2 Weeks Storage , Glass Bottle + 3 Weeks of Storage, a total of 12 treatments with 3 replications. The data were analyzed statistically with the F test at a 5% significance level. If F Count is greater than F Table 5%, then it is continued with the Duncan' New Multiple Range Test (DNMRT) at a level of 5%. The results showed that the treatment on several storage media had a significant effect on the simultaneous growth of cocoa seeds. And the length of time for storing seeds for one week was significantly different, there were parameters for observing seed germination, seed growing simultaneously and seed growing speed.
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Saakian, Alexander. « Effects of freezing seeds Abutilon theophrasti Medik. and Eruca vesicaria (L.) Cav. for the appearance, growth and development of plants ». АгроЭкоИнфо 4, no 46 (22 juillet 2021) : 7. http://dx.doi.org/10.51419/20214407.
Texte intégralRésumé :
A comparative study of the effect of different storage regimes of seeds (at + 5 ° С, -20 ° С and under deep freezing conditions at -196 ° С) of two cultivated plants of the Abutilon theophrasti Medik. and Eruca vesicaria (L.) Cav. on their viability and the development of plants from them in the field was carried out. Freezing increases the laboratory and field germination of the seeds of the A. theophrasti and does not significantly affect the germination of the seeds of the A. theophrasti, and also increases the germination energy of the seeds of both plant species. It did not cause the appearance of non-viable, ugly seedlings and plants. Plants went through a full development cycle and set full-value seeds. Biometric parameters of plants obtained from thawed seeds and control ones are very close. Cryopreservation is a promising way of storing seeds of the studied species, since it ensures longer periods of preservation of their viability. Keywords: ABUTILON THEOPHRASTI; ERUCA VESICARIA; SEED BANKS; LONG-TERM SEED STORAGE REGIMES; CRYOPRESERVATION
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BAUTISTA, D. A., J. CHEN, S. BARBUT et M. W. GRIFFITHS. « Use of an Autobioluminescent Salmonella Hadar To Monitor the Effects of Acid and Temperature Treatments on Cell Survival and Viability on Lactic Acid-Treated Poultry Carcasses ». Journal of Food Protection 61, no 11 (1 novembre 1998) : 1439–45. http://dx.doi.org/10.4315/0362-028x-61.11.1439.
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To determine the long-term effects of a lactic acid rinse on viability and recovery of pathogens, Salmonella Hadar was isolated from poultry and bioluminescent constructs obtained by transformation with the lux (CDABE) gene cassette from Photobacterium phosphoreum. Results indicated that the transformed Salmonella Hadar lux was otherwise phenotypically similar to the wild-type strain. Viability studies were performed by measuring luminescence following lactic acid treatment of turkey breast and subsequent storage at −12, 0, 5, and 10°C. The ability of the S. Hadar lux strain to recover was determined by monitoring light output after incubation at 22°C for 10 h. The results showed that metabolic activity was significantly (P < 0.05) affected by lactic acid and by storage temperatures of −12, 0, and 5°C. The lowest recovery rate was observed after rinsing with lactic acid and storing at 5°C. The study demonstrated that bacterial bioluminescence is an effective way of monitoring in “real time” the ability of bacteria to recover from stress.
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Kleeberger, Cynthia A., Robert H. Lyles, Joseph B. Margolick, Charles R. Rinaldo, John P. Phair et Janis V. Giorgi. « Viability and Recovery of Peripheral Blood Mononuclear Cells Cryopreserved for up to 12 Years in a Multicenter Study ». Clinical Diagnostic Laboratory Immunology 6, no 1 (1 janvier 1999) : 14–19. http://dx.doi.org/10.1128/cdli.6.1.14-19.1999.
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ABSTRACT The Multicenter AIDS Cohort Study (MACS), an ongoing prospective study of the natural history of human immunodeficiency virus (HIV), has stored biologic specimens, including peripheral blood mononuclear cells (PBMC), from 5,622 participants for up to 12 years. The purpose of the present analysis was to evaluate the quality of the PBMC in the MACS repository in order to test the validity and feasibility of nested retrospective studies and to guide the planning of future repositories. PBMC were collected from MACS participants at four centers at 6-month intervals from 1984 to 1995, cryopreserved, and transported to a central repository for storage. A total of 596 of these specimens were subsequently tested for viability and used to evaluate cell function, to conduct immunophenotype analysis, or to isolate HIV. Simple linear regression models were applied to evaluate trends in recovery and viability over time and by center. Results indicated that from a nominal 107 cells cryopreserved per vial at all four centers, the median number of viable cells recovered was at least 5 × 106 (50% of the number stored) and the median viability was at least 90%. Results suggested that cryopreserved cells can be stored for at least 12 years with no general tendency toward cell loss over time. Furthermore, there were no statistically significant changes in the percent cell viability according to the length of time frozen, regardless of HIV serostatus or the level of CD4+ lymphocytes. Storing 107 PBMC per vial yields sufficient viable cells for phenotypic and/or functional analysis. Results from the MACS provide the basis for the planning of future repositories for use by investigators with similar research goals.
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Mattis, Aidas, Diane Sempek et Suzanne Thibodeaux. « Effect of Long-Term Storage on Hematopoietic Progenitor Cell Transplant Product Quality ». American Journal of Clinical Pathology 152, Supplement_1 (11 septembre 2019) : S34—S35. http://dx.doi.org/10.1093/ajcp/aqz112.065.
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Abstract Hematopoietic progenitor cell (HPC) transplant product quality indicators after prolonged cryopreservation are variable. A recent study in Europe demonstrated wide variability of cryopreservation times, from 1 to >20 years, with most centers storing products for 5 or 10 years. Thawed HPC viability using various ex vivo means and in vivo by studying neutrophil and platelet engraftment has generally supported preservation of CD34+ viability with prolonged storage. However, sample size, methods, and timepoints varied, and clear guidelines currently do not exist regarding how long HPCs can be stored. This study’s objectives include an evaluation of HPC quality indicators over time and if HPC product age and/or viability correlates with HPC engraftment. Retrospective record review was conducted on 837 HPC products thawed between January 1, 2011, and December 31, 2018. HPC products were sampled and characterized at thawing by flow cytometry for total nucleated cell (TNC) recovery, CD34+ recovery, and CD34+ viability (by 7-AAD). Preestablished acceptability criteria for TNC recovery, CD34+ recovery, and CD34+ viability were >80%, >40%, and >60%, respectively. If available, products with ANC engraftment data were further analyzed to correlate data with time to HPC engraftment. HPC products were stored between 3 and 5,943 days; 816 products were infused, ranging from 3 to 5,273 days in storage. Most (81%) HPC transplants occurred within the first year, 47 infusions occurred after storage >5 years, and 4 infusions occurred after storage >10 years. CD34 viability and percent recovery of TNC and CD34 were not significantly different as time in storage increased. Given sample paucity, 19 samples stored for >10 years were analyzed in individual years and grouped. In total, 96.5% samples stored for <1 year and 68% in samples stored >10 years met acceptability criteria for >60% CD34+ recovery; 98.9% samples stored <1 year met the >60% CD34+ viability acceptability threshold versus 90% of samples stored for >10 years. The oldest sample infused after storage of 5,273 days (14.4 years) showed 42% TNC recovery, 19% CD34+ recovery, and 84% CD34 viability; ANC engraftment data were not available. Sixty-five samples containing ANC engraftment data showed that time to ANC engraftment ranged from 9 to 15 days. No clear trend was present for viability or CD34 recovery when compared with days to engraftment based on limited data. Although rare, infusion of HPC products of extended duration occurs. This study suggests that HPC product quality may decrease after extended cryopreservation based on preestablished acceptability criteria. The oldest sample infused had preserved CD34+ viability despite significantly less TNC and CD34+ recovery, suggesting thawing might select for more robust HPCs. More studies with older samples are needed to determine the clinical impact of cryopreservation and if modification of current quality indicators could be useful clinically.
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Steinberg, Jennifer Weathersbee, et Gayle M. Roux. « Midwestern Farm Widows : Adaptation Following Spousal Loss ». Nursing Science Quarterly 31, no 3 (19 juin 2018) : 296–304. http://dx.doi.org/10.1177/0894318418774949.
Texte intégralRésumé :
The purpose of this descriptive study was to co-create oral histories of Midwestern farm widows. Rural widows constitute a vulnerable population due to issues of bereavement and depression compounded by emotional and geographical isolation. A farm widow is often forced to maintain viability of the farm for the family’s livelihood. Oral history interviews with nine Midwest farm widows were conducted and analyzed. Three overarching themes emerged: competence, industriousness, and inner strength. Women shared stories of overcoming insurmountable obstacles. This study contributes to the literature on grief and expanding inner strength among rural widows. Further research could inform theory related to inner strength following a challenging life event.
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Tamas, Sophie. « Three Ways to Lose Your Epistemology ». International Review of Qualitative Research 2, no 1 (mai 2009) : 43–60. http://dx.doi.org/10.1525/irqr.2009.2.1.43.
Texte intégralRésumé :
This piece offers an autoethnographic examination of the unraveling of epistemic certainty. The reader is offered three stories of falling, which explain the author's loss of knowing as a result of education, trauma, or apostacy. The aporias and assumptions in each story are teased out as we explore what they profess, where they fall silent, and how they understand uncertainty. Rather than determining which story is true, the text asks ethical and practical questions about where each story takes us. The irreconcilability of these three narratives raises questions about the limits of pluralism, how we respond to ideological difference, and the viability of our need to know.
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Sobari, Iing, Sumadi Sumadi, Santi Rosniawaty et Edi Wardiana. « Perubahan Biokimia dan Indikator Vigor Benih Kakao pada Lima Taraf Lamanya Penyimpanan ». Jurnal Tanaman Industri dan Penyegar 7, no 3 (30 novembre 2020) : 163. http://dx.doi.org/10.21082/jtidp.v7n3.2020.p163-178.
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<em>During the storage period, cocoa seeds undergo various processes that will affect the content of their food reserves and will have an impact on germination capacity and seeds vigor. The objective of this study is to investigate the changes of biochemical and vigor of cocoa seeds after storage for a maximum of 4 weeks with an interval of 1 week. The experiment was conducted at the Integrated Laboratory and Greenhouse of Balittri, Sukabumi, West Java, from October to December 2019. The completely randomized design with five treatments of seeds storage duration: (a) seeds unstored, (b) seeds stored for 1 week, (c) seeds stored for 2 weeks, (d) seeds stored for 3 weeks, and (e) seeds stored for 4 weeks, and five replications were used in this study. Parameters observed during seed storage were the biochemistry change of seeds and the percentage of germinated seeds, while during seed germination is an indicator of seed vigor. The results showed that storing cocoa seeds for 1-4 weeks decreased the fat content of the seeds, while the free fatty acids, protein, and percentage of germinated seeds increased. Until the 21<sup>st</sup> day after sowing, seed storage increases electrical conductivity but decreases growth rate, maximum growth potential, viability, and hypocotyl length. Seeds stored for one week still good in their vigor (viability of 80%), while seeds stored for 2-4 weeks have decreased their vigor (maximum viability of 54.4%).</em>
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A. Offord, C., M. L. McKensy et P. V. Cuneo. « Critical review of threatened species collections in the New South Wales Seedbank : implications for ex situ conservation of biodiversity ». Pacific Conservation Biology 10, no 4 (2004) : 221. http://dx.doi.org/10.1071/pc040221.
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This article reviews the germinability and viability of seeds of threatened species collections in the New South Wales (NSW) Seedbank with the manifold aims of: ensuring that existing storage treatments and conditions provide effective ex situ storage of threatened species seed; providing baseline viability and seed storage life data on threatened species; and, identifying research gaps in seed germination and storage protocols for threatened species and communities. The germinability and viability of a range of seed accessions, of various ages and stored under different (although mainly identifiable) conditions in the NSW Seedbank, was determined through germination and cut-tests. The results indicated that many of the Fabaceae, Myrtaceae and Proteaceae species tested are orthodox and can be stored at 5 to 10% moisture content at 5�C for up to ten years without significant loss of viability (short- to medium-term storage). The best results were obtained in the lower seed moisture content range (2 to 9%), which appeared to be especially critical for long-term storage of many Proteaceae accessions, reinforcing the need to attain the correct seed moisture content for long-term storage. Around 10% of accessions exhibited some degree of dormancy even after long storage periods. Storage of the widest range of species, for periods greater than ten years for long-term conservation purposes, is generally best conducted by storing at sub-zero temperatures. Freezing at -18�C had little effect on the germinability of a range of seeds tested and is recommended over storage at 5�C. Collection and seed banking procedures for the NSW Seedbank will be regularly reviewed and procedures modified in order to identify the best long-term storage conditions for species within this and other seedbanks. Seed collection strategies to maximize diversity and uses of seedbanks in conservation are discussed.
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Adeli, Fahimeh, Ebrahim Zabihi, Zeinab Abedian, Samane Gharekhani, Mahdi Pouramir, Soraya Khafri et Maryam Ghasempour. « Comparative in vitro study of the effectiveness of Green tea extract and common storage media on periodontal ligament fibroblast viability ». European Journal of Dentistry 10, no 03 (juillet 2016) : 408–12. http://dx.doi.org/10.4103/1305-7456.184158.
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ABSTRACT Objective: Green tea extract (GTE) was shown to be effective in preserving periodontal ligament fibroblasts (PDLFs) of avulsed teeth. This study aimed at determining the potential of GTE in preserving the viability of PDLFs comparing with different storage media. Materials and Methods: Periodontal ligament cells were obtained from freshly extracted healthy impacted third molars and cultured in Dulbecco's Modified Eagle Medium (DMEM). Cell viability was determined by storing the cells in seven media; DMEM, tap water, Hank's balanced salt solution (HBSS), whole milk, hypotonic sucrose solution, GTE, and GTE + sucrose for 1, 2, 4, and 24 h at 37°C using tetrazolium salt-based colorimetric (3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyl tetrazolium bromide) assay. Statistical analysis was performed by one-way analysis of variance and post hoc tests. Results: GTE showed significantly higher protective effect than HBSS at 2, 4, and 24 h (P = 0.009, P = 0.02, P = 0.016), DMED at 2 h (P = 0.003), and milk at 4 h (P = 0.039). Conclusion: Although with undesirable osmolality and pH, GTE had a good ability in preserving the PDLFs comparing with other studied media.
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Adedayo, Adetumbi, et Olakojo Adelowo. « Storage Potentials and Tolerance of High Protein Maize (HPM) and Quality Protein Maize (QPM) to Seed Storage Pests in Controlled Environment ». Journal of Plant Protection Research 50, no 1 (1 mars 2010) : 67–71. http://dx.doi.org/10.2478/v10045-010-0012-6.
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Storage Potentials and Tolerance of High Protein Maize (HPM) and Quality Protein Maize (QPM) to Seed Storage Pests in Controlled EnvironmentStudies on seed storage were conducted at seed processing and storage section of the Institute of Agricultural Research and Training, Obafemi Awolowo University, Moor Plantation, Ibadan Nigeria in 2007 and 2008, to evaluate High protein maize (HPM) seed for resistance/tolerance to storage insect pest, and assess the resultant effect of seed treatment chemicals on its germination potential. Seeds of HPM variety (ART-98-SW1) and Quality protein maize (QPM) variety (ILE-1-OB) and two varieties of field corn (SUWAN-1-SR and TZPB-SRW) were treated with chemicals, namely Fitscophos™, Actellic 25 EC™, Apron Star™ and combination of Actellic 25 EC and Fitscophos before storing them under controlled environment for six months. The results show that HPM, QPM and field corn require storage chemicals for effective storage, but both QPM and HPM varieties were significantly damaged by storage insect pests compared to field corn varieties regardless of the chemical used. Apron Star effectively reduced infestation by insect pests, but it may bring about significant reduction in seed viability if used for long term storage. Combination of Actellic 25 EC and Fitscophos successfully reduced infestation by insect pests without significant injurious effect on seed viability. Breeding programmes for resistance to storage pests is recommended while long term effects of Apron Star on seed viability needs to be investigated before recommending it for use in HPM/QPM maize varieties.
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Liadi, Modinat, Andrew Collins, Ying Li et Daqing Li. « The Impact of Tissue Storage Conditions on Rat Olfactory Ensheathing Cell Yield and the Future Clinical Implications ». Cell Transplantation 27, no 9 (10 août 2018) : 1320–27. http://dx.doi.org/10.1177/0963689718787762.
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Trauma causes spinal cord injury, and the devastating consequences of the injury are due to the failure of the damaged central nervous system (CNS) axons to regenerate. Previous studies have shown that olfactory ensheathing cells (OECs) are a unique type of glial cell and they can promote regeneration of CNS axons to aid recovery after spinal cord injury. Transplantation of OECs, in particular from the olfactory bulb (OB), is considered one of the most promising therapeutic strategies for the repair of CNS injuries, including spinal cord injury. Transplantation of OECs can be autologous or allogenic. Here we focused on the less invasive and more error-proof allograft approach which needs a collection of donor OB tissue for OEC production. In this study, we investigated the effects on the yield and proportions of OECs and olfactory nerve fibroblasts (ONFs) from storing OB tissue in various media for periods of 24 and 48 hours. The OEC yield contributes to the viability of a successful cell transplant. We concluded that storing OB tissue for a period longer than 24 hours negatively impacted the total cell number and subsequently the OEC population. This study provides useful information for future clinical applications.
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م. حسين علي خضير بهاري الشويلي, م,. « Chekhov’s Protagonists in Contemporary Russian Writers’ Stories Hussein Ali Khudair Bahari ». لارك 4, no 43 (30 septembre 2021) : 1126–12. http://dx.doi.org/10.31185/lark.vol4.iss43.2044.
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The continuity of Chekov’s text adheres to the existence of his protagonists everywhere and then. The presence of Chekhov's heroes in every place and time is the key to the text's long-term viability. He described various characters, upon whom feelings were shown in different situations, specifically feeling scared among his protagonists. The character of (Belekov) in “The introversive man” who fears everything is different from the protagonist in “Khemech” by the contemporary writer Yuri Buyda. The protagonist’s wife made an unusual reaction against Chekov after her husband's death. She screamed: “I hate your writer Chekov! I hate him! I hate him!”. This reaction was a protest and a denial of the cover idea which had become a literary mode in literary works and daily life as was presented by Chekov in “The Introversive man”. The modern writer V. Bitsokh was able in “The cabinet” to show fear in people’s behavior in the Soviet Union, and so he did show how permanent fear of the protagonists’ environment was a common factor with Chekov’s. As for the female writer G. Shcherbakova in her “The introversive man”, she presented a character of a school headmistress named (Vania) who was dedicated and ambitious. However, she neglected an important aspect of her life, making a family. This was a struggle for her cover in Chekov. Shcherbakova named her story after Chekov’s “The introversive man”, but her protagonist differed from Chekov’s (Varinka) who did not recognize others’ feelings. Here, Chekov’s text gained its controversy and dialogue in a contemporary and modernist way.
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Kirpa, Mykola, Oleh Stasiv, Yuliya Bazileva et Oleh Kolisnyk. « WAYS OF STORING CORN GRAIN IN STORAGE OF VARIOUS TYPES ». Agriculture and Forestry, no 1 (23 mars 2021) : 155–69. http://dx.doi.org/10.37128/2707-5826-2021-12.
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The relevance of the study to establish methods of storing corn grain is related to the problem that exists in the technology of storage of crops of different crops. Methods and material and technical base with preservation do not fully take into account the characteristics of individual crops as objects of storage, which leads to loss of products and deterioration of its quality. There is also no technical and technological assessment of new methods of storage and granaries, including metal type. The article presents the results of research, which revealed the peculiarities of storage of corn grain in metal granaries. The temperature regime, which is formed in different places of the embankment and grain quality indicators - humidity, purity, acidity, viability (germination) depending on the thermal protection of the storage are established. Layers of grain embankment were found in the warehouse, where unfavorable conditions for preservation and stable condition of products are created. The technical and technological assessment was carried out and the efficiency of different granaries was determined depending on the storage and the direction of its use. As a result of the conducted researches the technical and technologicalcharacteristic of various ways applied to storage of grain is made, their advantages and lacks are established. For the purpose of safe storage in metal granaries it is recommended to carry out their thermal protection by drawing heat-insulating material on external walls of storage. For long-term storage of corn grain stocks, its humidity should not exceed 12% with the removal of the total mass of the unstable fraction. In the long run, it is necessary to reduce the storage of grain in a controlled gaseous environment, as one that increases a high degree of preservation of products and their quality.