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Articles de revues sur le sujet « UCOE »

Auteur : Grafiati

Publié le 9 mars 2023

Mis à jour le 31 juillet 2025

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1

Nelson, Everette J. R., Laura M. Tuschong, and Dennis D. Hickstein. "Lentiviral Vectors Incorporating Ubiquitous Chromatin Opening Element Driving Canine CD18 Expression." Blood 128, no. 22 (2016): 5890. http://dx.doi.org/10.1182/blood.v128.22.5890.5890.

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Abstract Leukocyte adhesion deficiency type 1 (LAD1) in humans is caused due to mutations in the ITGB2 gene encoding the leukocyte CD18 subunit (b2 integrin). This results in defective leukocyte adhesion and migration leading to recurrent episodes of life-threatening bacterial infection. Canine leukocyte adhesion deficiency (CLAD) represents a disease-specific large animal model of LAD1 in which new therapeutic approaches could be tested. Our previous studies have demonstrated variable efficiency of CD18 expression under the control of several promoters. These include cellular promoters such a

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Ikawa, Yasuhiro, Toru Uchiyama, Guridevi Jayashree Jagadeesh, and Fabio Candotti. "Comparison of Immortalization Potential of Gamma-Retroviral, Lentiviral and Foamy Virus Gene Transfer Vectors." Blood 118, no. 21 (2011): 3116. http://dx.doi.org/10.1182/blood.v118.21.3116.3116.

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Abstract Abstract 3116 Therapeutic gene transfer has been used successfully to treat a variety of human genetic diseases. Although protocols have shown positive clinical outcomes, the success of clinical trials were tempered by adverse events, in which integration of the viral vectors increased transcription of cancer-related genes and thereby contributed to development of leukemia. In all documented cases of insertional mutagenesis, the viral vectors used contained full-length, gamma-retrovirus long terminal repeats (LTRs) that are able to exert strong promoter and enhancer activity driving n

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Boscolo, Sabrina, Francesca Mion, Marta Licciulli, et al. "Simple scale-up of recombinant antibody production using an UCOE containing vector." New Biotechnology 29, no. 4 (2012): 477–84. http://dx.doi.org/10.1016/j.nbt.2011.12.005.

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Zhang, Fang, Susannah I. Thornhill, Steven J. Howe, et al. "Lentiviral vectors containing an enhancer-less ubiquitously acting chromatin opening element (UCOE) provide highly reproducible and stable transgene expression in hematopoietic cells." Blood 110, no. 5 (2007): 1448–57. http://dx.doi.org/10.1182/blood-2006-12-060814.

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AbstractUbiquitously acting chromatin opening elements (UCOEs) consist of methylation-free CpG islands encompassing dual divergently transcribed promoters of housekeeping genes that have been shown to confer resistance to transcriptional silencing and to produce consistent and stable transgene expression in tissue culture systems. To develop improved strategies for hematopoietic cell gene therapy, we have assessed the potential of the novel human HNRPA2B1-CBX3 UCOE (A2UCOE) within the context of a self-inactivating (SIN) lentiviral vector. Unlike viral promoters, the enhancer-less A2UCOE gave

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Guichardaz, Michelle, Sveva Bottini, Elisa Balmas, and Alessandro Bertero. "Overcoming the Silencing of Doxycycline-Inducible Promoters in hiPSC-derived Cardiomyocytes." Open Research Europe 4 (December 18, 2024): 266. https://doi.org/10.12688/openreseurope.19024.1.

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Background Human induced pluripotent stem cells (hiPSCs) are pivotal for studying human development, modeling diseases, and advancing regenerative medicine. Effective control of transgene expression is crucial to achieve temporal and quantitative precision in all of these contexts. The doxycycline (dox)-inducible OPTi-OX system, which integrates the Tet-On 3G transactivator and dox-responsive transgene at the hROSA26 and AAVS1 genomic safe harbors (GSHs), respectively, offers a promising solution. Yet, transgene silencing, particularly in hiPSC-derived cardiomyocytes (hiPSC-CMs), limits its ut

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Pfaff, Nils, Nico Lachmann, Mania Ackermann, et al. "The Ubiquitous Chromatin Opening Element (UCOE) Enhances Lentiviral Cytidine Deaminase (CDD) Expression and Drug Resistance During Hematopoietic Differentiation of Murine Induced Pluripotent Stem Cells (iPSCs),." Blood 118, no. 21 (2011): 4179. http://dx.doi.org/10.1182/blood.v118.21.4179.4179.

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Abstract Abstract 4179 Introduction: Transfer of drug resistance genes to the hematopoietic system has been advocated for myeloprotection during anti-cancer chemotherapy, however, for malignancies manifestated in blood or bone marrow compartments this strategy carries the risk of inadvertent transduction of tumor cells. Hematopoietic differentiation of induced pluripotent stem cells (iPSCs) has the potential to overcome this problem, and we here have investigated this concept in the context of Cytidine Deaminase (CDD)-mediated myeloprotection. However, epigenetic silencing of transgenic promot

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Brendel, Christian, Stefan Stein, Michael Antoniou, and Manuel Grez. "UCOE (ubiquitous chromatin opening element) mediates copy dependent expression of gp91phox in lentiviral vectors." Blood Cells, Molecules, and Diseases 40, no. 2 (2008): 257–58. http://dx.doi.org/10.1016/j.bcmd.2007.10.025.

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Hou, Jeff Jia Cheng, Ben S. Hughes, Matthew Smede, et al. "High-throughput ClonePix FL analysis of mAb-expressing clones using the UCOE expression system." New Biotechnology 31, no. 3 (2014): 214–20. http://dx.doi.org/10.1016/j.nbt.2014.02.002.

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Betts, Zeynep, Alexandra S. Croxford, and Alan J. Dickson. "Evaluating the interaction between UCOE and DHFR-linked amplification and stability of recombinant protein expression." Biotechnology Progress 31, no. 4 (2015): 1014–25. http://dx.doi.org/10.1002/btpr.2083.

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Pradhan, Ajoya Kumar, Mahendra Kumar Mohanty, and Sanjeeb Kumar Kar. "Techno-economic Evaluation of Stand-alone Hybrid Renewable Energy System for Remote Village Using HOMER-pro Software." International Journal of Applied Power Engineering (IJAPE) 6, no. 2 (2017): 73. http://dx.doi.org/10.11591/ijape.v6.i2.pp73-88.

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The off-grid hybrid renewable energy generation system has lesser cost of energy with higher reliability when compared with solar Photovoltaic (PV) or wind energy system individually. The optimization design is worked out by reducing the Unit Cost Of Energy (UCOE) for different case studies and comparing the outcomes obtained by the use of HOMER-Pro (hybrid optimization model of electric renewable) software. The optimal cash flow analysis of hybrid energy system is based on the load patterns is discussed, solar irradiance (kW/m2) of site at proper latitude and longitude, wind speed and price o

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Pradhan, Ajoya Kumar, Mahendra Kumar Mohanty, and Sanjeeb Kumar Kar. "Techno-Economic Evaluation of Stand-alone Hybrid Renewable Energy System for Remote Village Using HOMER-Pro Software." International Journal of Applied Power Engineering (IJAPE) 6, no. 2 (2017): 74. http://dx.doi.org/10.11591/ijape.v6.i2.pp74-89.

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The off-grid hybrid renewable energy generation system has lesser cost of energy with higher reliability when compared with solar photovoltaic (PV) or wind energy system individually. The optimization design is worked out by reducing the unit cost of energy (UCOE) for different case studies and comparing the outcomes obtained by the use of HOMER-Pro (Hybrid Optimization Model of Electric Renewable) software. The optimal cash flow analysis of hybrid energy system is based on the load patterns is discussed, solar irradiance (kW/m2) of site at proper latitude and longitude, wind speed and price o

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Anakok, O. F., K. N. Bayindirli, and P. Kose. "Optimising the new ucoe models as higher direct transgene expression profile for effective gene therapy applications." Cytotherapy 23, no. 5 (2021): S151—S152. http://dx.doi.org/10.1016/s1465324921005284.

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Seymour, Brenda J., Swati Singh, Hannah M. Certo, et al. "Effective, safe, and sustained correction of murine XLA using a UCOE-BTK promoter-based lentiviral vector." Molecular Therapy - Methods & Clinical Development 20 (March 2021): 635–51. http://dx.doi.org/10.1016/j.omtm.2021.01.007.

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Zhang, Fang, Amy R. Frost, Mike P. Blundell, Olivia Bales, Michael N. Antoniou, and Adrian J. Thrasher. "A Ubiquitous Chromatin Opening Element (UCOE) Confers Resistance to DNA Methylation–mediated Silencing of Lentiviral Vectors." Molecular Therapy 18, no. 9 (2010): 1640–49. http://dx.doi.org/10.1038/mt.2010.132.

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Betts, Zeynep, and Alan J. Dickson. "Assessment of UCOE on Recombinant EPO Production and Expression Stability in Amplified Chinese Hamster Ovary Cells." Molecular Biotechnology 57, no. 9 (2015): 846–58. http://dx.doi.org/10.1007/s12033-015-9877-y.

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Fitriani, R., I. N. Fitriani, N. Putri, W. C. Amalia, and A. Surjosatyo. "Used Cooking Oil Methyl Esther (UCOME) Production on a Pilot Scale in Bali Island, Indonesia, and Its Utilization as a Component Blending for Bio-Marine Fuel Oil." IOP Conference Series: Earth and Environmental Science 1395, no. 1 (2024): 012012. http://dx.doi.org/10.1088/1755-1315/1395/1/012012.

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Abstract Indonesia aims to achieve a New & Renewable Energy mix of 23% by 2025, so it is necessary to have policies related to the use of complementary feedstocks in the biofuel development program in Indonesia. Used Cooking Oil (UCO) is a domestic waste with the potential to be utilized as a complementary feedstock due to its abundant availability and almost zero carbon emission. However, to assess the sustainability of UCO utilization, especially as a component blending for Bio-Marine Fuel Oil, it is necessary to identify UCO characteristics and quality of its product, called as UCOME, a

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Allen, Marianne Lindahl, and Michael Antoniou. "Correlation of DNA Methylation with Histone Modifications Across the HNRPA2B1-CBX3 Ubiquitously-Acting Chromatin Open Element (UCOE)." Epigenetics 2, no. 4 (2007): 227–36. http://dx.doi.org/10.4161/epi.2.4.5231.

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Dighe, Niraja, Maroun Khoury, Citra Mattar, et al. "Long-Term Reproducible Expression in Human Fetal Liver Hematopoietic Stem Cells with a UCOE-Based Lentiviral Vector." PLoS ONE 9, no. 8 (2014): e104805. http://dx.doi.org/10.1371/journal.pone.0104805.

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Dharshanan, Suba, Heilly Chong, Swee Hung Cheah, and Zulkeflie Zamrod. "Stable expression of H1C2 monoclonal antibody in NS0 and CHO cells using pFUSE and UCOE expression system." Cytotechnology 66, no. 4 (2013): 625–33. http://dx.doi.org/10.1007/s10616-013-9615-x.

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Haryani, Nina, Elfrida Rasyidah Desvi Imanda, and Andiga Asih Ambarwati Utami. "Pengaruh Kadar Free Fatty Acid dalam Used Cooking Oil (UCO) dan Massa Katalis pada Proses Transesterifikasi terhadap Karakteristik dan Kelimpahan Used Cooking Oil Methyl Ester (UCOME)." ALCHEMY Jurnal Penelitian Kimia 21, no. 1 (2025): 94. https://doi.org/10.20961/alchemy.21.1.84661.94-103.

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<p>Salah satu pemanfaatan kembali minyak jelantah atau <em>Used Cooking Oil </em>(UCO) adalah diolah menjadi biodiesel <em>Used Cooking Oil Methyl Ester </em>(UCOME). <em>Free Fatty Acid</em> (FFA) dalam UCO diolah melalui reaksi transesterifikasi menjadi metil ester. Penelitian ini bertujuan untuk mempelajari pengaruh kadar FFA UCO dan massa katalis terhadap karakteristik dan <em>yield </em>UCOME yang dihasilkan. Variasi kadar FFA UCO yang digunakan yaitu 1,493%; 1,536%; 2,56%; dan 5,504%. Reaksi transesterifikasi dilakukan pada temperatur

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Yu-cheng Kao, Vincent, Sonia Ferreira, Simon N. Waddington, and Michael N. Antoniou. "Haemophilia B Curative FIX Production from a Low Dose UCOE-based Lentiviral Vector Following Hepatic Pre-natal Delivery." Current Gene Therapy 16, no. 4 (2016): 231–41. http://dx.doi.org/10.2174/1566523216666161102150101.

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Saunders, Fay, Berni Sweeney, Michael N. Antoniou, Paul Stephens, and Katharine Cain. "Chromatin Function Modifying Elements in an Industrial Antibody Production Platform - Comparison of UCOE, MAR, STAR and cHS4 Elements." PLOS ONE 10, no. 4 (2015): e0120096. http://dx.doi.org/10.1371/journal.pone.0120096.

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Cullmann, Katharina, Kaj E. C. Blokland, Attila Sebe, et al. "Sustained and regulated gene expression by Tet-inducible “all-in-one” retroviral vectors containing the HNRPA2B1-CBX3 UCOE®." Biomaterials 192 (February 2019): 486–99. http://dx.doi.org/10.1016/j.biomaterials.2018.11.006.

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Doan, Chinh Chung, Thanh Long Le, Nguyen Quynh Chi Ho та Nghia Son Hoang. "Effects of ubiquitous chromatin opening element (UCOE) on recombinant anti-TNFα antibody production and expression stability in CHO-DG44 cells". Cytotechnology 74, № 1 (2021): 31–49. http://dx.doi.org/10.1007/s10616-021-00503-1.

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Watson, Carole S., Rachel Schaefer, Susan E. White, et al. "Effect of intermittent umbilical cord occlusion on fetal respiratory activity and brain adenosine in late-gestation sheep." Reproduction, Fertility and Development 14, no. 1 (2002): 35. http://dx.doi.org/10.1071/rd01013.

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It was hypothesized that intermittent umbilical cord occlusion (UCO) would inhibit ovine fetal breathing movements (FBM) in association with increased cerebral adenosine levels. To test this hypothesis, on two successive days during late gestation (133–134 days; term = 146 days), microdialysis samples were collected from the brains of 10 chronically instrumented fetal sheep during 2-h periods of complete UCO induced every 30 min (Day 1: 2-min UCOs; Day 2: 4-min UCOs). Control fetuses (n = 10) underwent no UCO. Tracheal pressure was measured throughout. This regimen resulted in a decrease in fe

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Frank, E., and S. O. Effiom. "Development of a multigeneration system with integrated hydrogen production: A typical analysis." Nigerian Journal of Technology 42, no. 3 (2023): 330–38. http://dx.doi.org/10.4314/njt.v42i3.5.

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Rise in demand for energy and emission from fossil fuels has become a thing of concern to researchers and Engineers. It is one thing to provide the energy needed by the society, and another to produce a clean and eco-friendly power supply. In this study, a thermodynamic and economic modelling of a solar-driven multigeneration power plant (MGPP) integrated with a PEM Electrolyzer for hydrogen production is examined. The performance indicators considered include energy and exergy efficiencies, net work, energetic and exergetic COP, cooling rate. Results of the thermodynamic analysis show that th

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Müller-Kuller, Uta, Mania Ackermann, Stephan Kolodziej, et al. "A minimal ubiquitous chromatin opening element (UCOE) effectively prevents silencing of juxtaposed heterologous promoters by epigenetic remodeling in multipotent and pluripotent stem cells." Nucleic Acids Research 43, no. 3 (2015): 1577–92. http://dx.doi.org/10.1093/nar/gkv019.

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Lear, Christopher A., Laura Bennet, Benjamin A. Lear, Jenny A. Westgate, and Alistair J. Gunn. "Lack of evidence for impaired preload or Bezold-Jarisch activation during brief umbilical cord occlusions in fetal sheep." American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 320, no. 4 (2021): R532—R540. http://dx.doi.org/10.1152/ajpregu.00357.2020.

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Impaired cardiac preload secondary to umbilical cord occlusion (UCO) has been hypothesized to contribute to intrapartum decelerations, brief falls in fetal heart rate (FHR), through activation of the Bezold-Jarisch reflex. This cardioprotective reflex increases parasympathetic and inhibits sympathetic outflows triggering hypotension, bradycardia, and peripheral vasodilation, but its potential to contribute to intrapartum decelerations has never been systematically examined. In this study, we performed bilateral cervical vagotomy to remove the afferent arm and the efferent parasympathetic arm o

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Anakok, O. F., P. Kose, and K. N. Bayindirli. "Gene Editing/Gene Therapies: BIOREACTOR RECOMBINANT PRODUCTION OF SARS-COV-2 VIRUS ANTIGENS IN CHO CELL CLONES BY USING NEW UCOE LENTIVIRAL VECTOR SYSTEM." Cytotherapy 25, no. 6 (2023): S258—S259. http://dx.doi.org/10.1016/s1465-3249(23)00636-9.

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Sapee, Syazwana, Ahmad Fitri Yusop, Mohammad Nazri Mohd Jaafar, et al. "Synthesis of non-edible biodiesel from crude jatropha oil and used cooking oil." MATEC Web of Conferences 225 (2018): 06008. http://dx.doi.org/10.1051/matecconf/201822506008.

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This study focuses on a feasibility study of alternative nonedible crude oil such as jatropha and used cooking oil in biodiesel production. Crude jatropha oil (CJO) and used cooking oil (UCO) were converted to biodiesel using a two-step transesterification process with presents of acid-based and alkaline-based catalysts. Each three biodiesel blends (B5, B15 and B25) have been produced by blended with conventional diesel fuel (CDF). Determination of the fuel properties for each blend including CDF, Jatropha Methyl Ester (JME) and Used Cooking Oil Methyl Ester (UCOME) have been carried out. The

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Green, LR, Y. Kawagoe, DJ Hill, BS Richardson, and VK Han. "The effect of intermittent umbilical cord occlusion on insulin-like growth factors and their binding proteins in preterm and near-term ovine fetuses." Journal of Endocrinology 166, no. 3 (2000): 565–77. http://dx.doi.org/10.1677/joe.0.1660565.

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Intermittent umbilical cord compression with resultant fetal hypoxia can have a negative impact on fetal growth and development. Insulin-like growth factors (IGFs) and their binding proteins (IGFBPs) are the most important regulators of fetal growth. In preterm (107-108 days of gestation) and near-term (128-131 days of gestation) ovine fetuses, we have determined the effect of intermittent umbilical cord occlusion (UCO) over a period of 4 days on the profile and expression of IGFs and IGFBPs. In experimental group animals (preterm n=7; near term n=7) UCOs were carried out by complete inflation

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Lear, Christopher A., Michael J. Beacom, Michi Kasai, et al. "Circulating catecholamines partially regulate T-wave morphology but not heart rate variability during repeated umbilical cord occlusions in fetal sheep." American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 319, no. 1 (2020): R123—R131. http://dx.doi.org/10.1152/ajpregu.00026.2020.

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Fetal heart rate (FHR) variability (FHRV) and ST segment morphology are potential clinical indices of fetal well-being during labor. β-Adrenergic stimulation by circulating catecholamines has been hypothesized to contribute to both FHRV and ST segment morphology during labor, but this has not been tested during brief repeated fetal hypoxemia that is characteristic of labor. Near-term fetal sheep (0.85 gestation) received propranolol (β-adrenergic blockade; n = 10) or saline ( n = 7) 30 min before being exposed to three 2-min complete umbilical cord occlusions (UCOs) separated by 3-min reperfus

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Mitra, Abhas, and Krishna Kumar Singh. "On the Maximum Energy Release from Formation of Static Compact Objects." Galaxies 11, no. 6 (2023): 116. http://dx.doi.org/10.3390/galaxies11060116.

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Type II Supernova 1987A (SN 1987A), observed in 1987, released an energy of Q≈3×1053 erg. This huge energy is essentially the magnitude of gravitational potential or self-gravitational energy (PE) of a new born cold neutron star having a gravitational compactness or redshift zb≈0.15. One may wonder what could be the upper limit on the amount of energy that might be released with the formation of a cold Ultra Compact Object (UCO) with an arbitrary high zb. Accordingly, here, for the first time, we obtain an analytical expression for the PE of a homogeneous general relativistic UCO assuming it t

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Ikawa, Yasuhiro, Toru Uchiyama, Guridevi Jayashree Jagadeesh, and Fabio Candotti. "Negative Control Region Is a Critical Element Of Insertional Oncogenesis After Gene Transfer Into Hematopoietic Progenitors With Moloney Murine Leukemia Viruses." Blood 122, no. 21 (2013): 164. http://dx.doi.org/10.1182/blood.v122.21.164.164.

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Abstract Gene transfer into hematopoietic stem cells has been used successfully to treat a variety of human genetic diseases. Although protocols have shown positive clinical outcomes, the successes of clinical trials have been tempered by adverse events in which the use of gamma-retroviral vectors (GV) containing full-length long terminal repeats (LTRs) with strong enhancer activity increased transcription of cancer-related genes, and thereby contributed to development of leukemia. Assessing safety of integrating viral vectors for future clinical use is therefore of paramount importance. The n

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Bruun, Nina, Juho Lehmusto, Jarl Hemming, Fiseha Tesfaye, and Leena Hupa. "Metal Rod Surfaces after Exposure to Used Cooking Oils." Sustainability 14, no. 1 (2021): 355. http://dx.doi.org/10.3390/su14010355.

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Used cooking oils (UCOs) have a high potential as renewable fuels for the maritime shipping industry. However, their corrosiveness during storage and usage are some of the concerns yet to be investigated for addressing compatibility issues. Thus, the corrosion of steels and copper exposed to the UCOs was studied through the immersion of metal rods for different periods. The changes on the rod surfaces were analyzed with a scanning electron microscope (SEM). After the immersion, the copper concentration dissolved in the bio-oils was measured using inductively coupled plasma-optical emission spe

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Borges, Alessandra Buhler, Carlos Rocha Gomes Torres, Graziela Ribeiro Batista, Eduardo Bresciani, Erica Crastechini, and Rayssa Ferreira Zanatta. "Bond Strength of Reline Resins to Aged-simulated Denture Base Acrylic Resin." World Journal of Dentistry 7, no. 1 (2016): 1–5. http://dx.doi.org/10.5005/jp-journals-10015-1353.

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ABSTRACT Objectives The aim of this study was to evaluate the bond strength of different direct reliners to acrylic resin for denture base. Materials and methods Double-cone specimens were made: HA-heat-cured acrylic resin-(n = 20); U-Ufi Gel Hard C-(n = 10); K: Kooliner-(n = 10); R-Rebase II Fast-(n = 10) and RH-Rebase II Fast + Resin Hardener-(n = 10). Ten HA samples were immediately submitted to cohesive test. The remaining HA samples and others were submitted to thermal aging (HAaged, 1000 cycles, 5.55oC), followed by tensile test. For tensile strength, 50 single cone-shaped samples were m

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Lear, Christopher A., Jenny A. Westgate, Michi Kasai, et al. "Parasympathetic activity is the key regulator of heart rate variability between decelerations during brief repeated umbilical cord occlusions in fetal sheep." American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 319, no. 5 (2020): R541—R550. http://dx.doi.org/10.1152/ajpregu.00186.2020.

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Fetal heart rate variability (FHRV) is a widely used index of intrapartum well being. Both arms of the autonomic system regulate FHRV under normoxic conditions in the antenatal period. However, autonomic control of FHRV during labor when the fetus is exposed to repeated, brief hypoxemia during uterine contractions is poorly understood. We have previously shown that the sympathetic nervous system (SNS) does not regulate FHRV during labor-like hypoxia. We therefore investigated the hypothesis that the parasympathetic system is the main mediator of intrapartum FHRV. Twenty-six chronically instrum

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Izmaylov, M., D. Rafaja, V. Sechovský, and A. V. Andreev. "Ferromagnetism in UCo1−xMnxAl and UCo1−xVxAl." Czechoslovak Journal of Physics 52, S1 (2002): A269—A272. http://dx.doi.org/10.1007/s10582-002-0065-5.

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Nikishina, Olga, and Olga Nikishina. "Incomplete construction: Russian and foreign experience." MATEC Web of Conferences 212 (2018): 04007. http://dx.doi.org/10.1051/matecconf/201821204007.

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The study is devoted to the objects of unfinished construction (hereinafter - UCO). The problem of the UCOs is urgent both for the regions and for the Russian Federation as a whole. The main reasons for the large number of the unfinished construction objects are analyzed in the paper. The global experience of solving the issue of long-term construction is considered. The unfinished objects spoil the architectural outlook of the city, while the lands are used inefficiently and the necrosis of capital occurs. In Russia, as a rule, conservation of these objects is not done that creates a real thr

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Arponen, Milja, Eeva-Christine Brockmann, Riku Kiviranta, Urpo Lamminmäki, and Kaisa K. Ivaska. "Recombinant Antibodies with Unique Specificities Allow for Sensitive and Specific Detection of Uncarboxylated Osteocalcin in Human Circulation." Calcified Tissue International 107, no. 6 (2020): 529–42. http://dx.doi.org/10.1007/s00223-020-00746-8.

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Abstract Osteocalcin is a bone-specific protein which contains three glutamic acid residues (Glu) that undergo post-translational gamma-carboxylation. Uncarboxylated osteocalcin (ucOC) may participate in the regulation of glucose metabolism, thus measurement of ucOC could be useful in evaluating interactions between bone and glucose metabolism. We developed recombinant antibodies and immunoassay to specifically detect ucOC in human blood samples. ucOC-specific recombinant antibodies were selected from an antibody library by phage display. Four candidates were characterized, and one (Fab-AP13)

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Tacey, Alexander, Cassandra Smith, Mary N. Woessner, et al. "Undercarboxylated osteocalcin is associated with vascular function in female older adults but does not influence vascular function in male rabbit carotid artery ex vivo." PLOS ONE 15, no. 11 (2020): e0242774. http://dx.doi.org/10.1371/journal.pone.0242774.

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Background There are conflicting reports on the association of undercarboxylated osteocalcin (ucOC) in cardiovascular disease development, including endothelial function and hypertension. We tested whether ucOC is related to blood pressure and endothelial function in older adults, and if ucOC directly affects endothelial-mediated vasodilation in the carotid artery of rabbits. Methods In older adults, ucOC, blood pressure, pulse wave velocity (PWV) and brachial artery flow-mediated dilation (BAFMD) were measured (n = 38, 26 post-menopausal women and 12 men, mean age 73 ± 0.96). The vasoactivity

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Chen, Yi-Chen, Ryoya Oga, Takahiro Furumi, Koki Nakagawa, Yoshihiro Nita, and Hiroyuki Tamaki. "The Effect of Bone Mechanical Stress Caused by Electrical Stimulation-Induced Muscle Contraction on Osteocalcin Secretion." Biology 13, no. 11 (2024): 882. http://dx.doi.org/10.3390/biology13110882.

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Electrical stimulation-induced muscle contraction (ESMC) has demonstrated various physiological benefits, but its effects on the secretion of undercarboxylated osteocalcin (ucOC), a bone-derived cytokine, remain unclear. This study explored the relationship between ESMC, bone strain, and ucOC secretion through two experiments. In the first, young male Fischer 344 rats were divided into three groups: low-frequency ES (LF, 10 Hz), high-frequency ES (HF, 100 Hz), and control (CON). Acute 30-min transcutaneous ES was applied, and both bone strain and ucOC levels were measured. In the second experi

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Liu, Xiaoying, Bu B. Yeap, Kaye E. Brock, et al. "Associations of Osteocalcin Forms With Metabolic Syndrome and Its Individual Components in Older Men: The Health In Men Study." Journal of Clinical Endocrinology & Metabolism 106, no. 9 (2021): e3506-e3518. http://dx.doi.org/10.1210/clinem/dgab358.

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Abstract Context The osteoblast-derived polypeptide, osteocalcin (OC), has been associated with lower risk of type 2 diabetes and metabolic syndrome (MetS) in several epidemiological studies. Animal studies have indicated the undercarboxylated form of OC (ucOC) drives its association with metabolic outcomes. Objective We compared associations of ucOC and carboxylated OC (cOC) with MetS and its components in older men. Methods A cross-sectional analysis of 2575 men aged ≥70 years and older resident in Perth, Western Australia. ucOC was assayed using a hydroxyapatite-binding method, and cOC calc

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McKenzie, Amy L., and Lawrence E. Armstrong. "Monitoring Body Water Balance in Pregnant and Nursing Women: The Validity of Urine Color." Annals of Nutrition and Metabolism 70, Suppl. 1 (2017): 18–22. http://dx.doi.org/10.1159/000462999.

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Background: Urine osmolality (UOSM) reflects the renal regulation of excess fluid or deficit fluid, and therefore, serves as a marker of hydration status. Little is known about monitoring hydration in pregnant and lactating women despite significant physiological challenges to body water balance during that time. Therefore, we designed a study to assess if urine color (UCOL), an inexpensive and practical method, was a valid means of assessing urine concentration. Twenty-four hour UCOL was significantly correlated with 24 h UOSM in all women: pregnant, lactating, and control (r = 0.61-0.84, all

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Nimptsch, Katharina, Alexandra Nieters, Susanne Hailer, Günther Wolfram, and Jakob Linseisen. "The association between dietary vitamin K intake and serum undercarboxylated osteocalcin is modulated by vitamin K epoxide reductase genotype." British Journal of Nutrition 101, no. 12 (2008): 1812–20. http://dx.doi.org/10.1017/s0007114508131750.

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Vitamin K acts as a cofactor during the γ-carboxylation of vitamin K-dependent proteins. Undercarboxylated osteocalcin (ucOC) is a suggested biomarker of vitamin K status. The +2255 polymorphism of the vitamin K epoxide reductase gene (VKORC1) was shown to be associated with the recycling rate of the active form of vitamin K. We investigated the association between dietary vitamin K intake and serum ucOC and hypothesized that this association might vary byVKORC1genotype. ucOC and total intact osteocalcin (iOC) concentrations were quantified using specific ELISA tests in serum samples of 548 ma

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Yang, Jianjian, Changsong Xie, Qianqian Yang, et al. "PANa/Covalent organic framework composites with improved water uptake and proton conductivity." Chemical Communications 58, no. 8 (2022): 1131–34. http://dx.doi.org/10.1039/d1cc06010d.

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In this communication, we construct composite-type PANa@UCOF-x by the in situ reaction strategy, which combines the advantages of UCOF with ordered channels and PANa with high water uptake, thus improving the proton conductivity of UCOF.

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Gasparini, A., Y. K. Huang, N. T. Huy, et al. "The Superconducting Ferromagnet UCoGe." Journal of Low Temperature Physics 161, no. 1-2 (2010): 134–47. http://dx.doi.org/10.1007/s10909-010-0188-1.

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FUJINO, Masato, Kazuo SHINOZAKI, Yukikazu NATORI, and Kazuhisa OHTAGUCHI. "The concept of UCEE Researcher Database." Journal of Information Processing and Management 50, no. 5 (2007): 266–79. http://dx.doi.org/10.1241/johokanri.50.266.

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Ellis, Lindsay A., Brandon A. Yates, Amy L. McKenzie, Colleen X. Muñoz, Douglas J. Casa, and Lawrence E. Armstrong. "Effects of Three Oral Nutritional Supplements on Human Hydration Indices." International Journal of Sport Nutrition and Exercise Metabolism 26, no. 4 (2016): 356–62. http://dx.doi.org/10.1123/ijsnem.2015-0244.

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Urine color (Ucol) as a hydration assessment tool provides practicality, ease of use, and correlates moderately to strongly with urine specific gravity (Usg) and urine osmolality (Uosm). Indicative of daily fluid turnover, along with solute and urochrome excretion in 24-hr samples, Ucol may also reflect dietary composition. Thus, the purpose of this investigation was to determine the efficacy of Ucol as a hydration status biomarker after nutritional supplementation with beetroot (880 mg), vitamin C (1000 mg), and riboflavin (200 mg). Twenty males (Mean ± SD; age, 21 ± 2 y; body mass, 82.12 ± 1

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Dong, Jae-June, Jay J. Shen, and Yong-Jae Lee. "Dose-Dependent Effect of Cotinine-Verified Tobacco Smoking on Serum Immunoglobulin E Levels in Korean Adult Males." Nicotine & Tobacco Research 21, no. 6 (2017): 813–17. http://dx.doi.org/10.1093/ntr/ntx247.

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Abstract Background Smoking is one of the risk factors to exacerbate allergic diseases, and it may affect serum immunoglobulin E (IgE) levels. However, few studies have relied on an objective biomarker to examine the effect of tobacco smoking on serum IgE levels. Method A nationwide cross-sectional study was conducted to examine the relationship between urinary cotinine (Ucot) concentrations and IgE levels in 973 males using data from the 2010 Korean National Health and Nutrition Examination Survey (KNHANES). Ucot was classified into four groups based on concentration (ng/mL) as follows: nonsm

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