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1
Moyo, P., E. Allsopp, F. Roets, L. Mostert et F. Halleen. « Arthropods Vector Grapevine Trunk Disease Pathogens ». Phytopathology® 104, no 10 (octobre 2014) : 1063–69. http://dx.doi.org/10.1094/phyto-11-13-0303-r.
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Arthropod-mediated dispersal of pathogens is known in many cropping systems but has never been demonstrated for grapevine trunk disease pathogens. Arthropods from vineyards were screened for the presence of pathogens associated with Petri disease and esca using cultural and molecular techniques. The ability of the most abundant pathogen-carrying species to inoculate healthy grapevine vascular tissues was also determined. Millipedes and ants were allowed to associate with a DsRed- Express-transformed Phaeomoniella chlamydospora, after which they were exposed to freshly pruned healthy grapevines under controlled conditions and wounds were monitored for subsequent infection. In addition, the possibility of millipede excreta, commonly found on pruning wounds in the field, to act as inoculum source was determined. A diverse arthropod fauna was associated with declining grapevines and many of these carried trunk disease pathogens. However, spiders, the ant Crematogaster peringueyi, and the millipede Ommattoiulus moreleti were the most abundant pathogen carriers. The ant and millipede species fed on pruning wound sap and effectively transmitted trunk disease pathogens. Millipede excreta contained viable spores of Phaeomoniella chlamydospora and may serve as an inoculum source. Numerous arthropods, including beneficial predators, are potential vectors of grapevine trunk disease pathogens. Our results highlight the need for an integrated approach, including targeted management of ants and millipedes at the time of pruning, to limit the spread of grapevine trunk diseases.
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Magnin-Robert, Maryline, Alessandro Spagnolo, Anna Boulanger, Cécile Joyeux, Christophe Clément, Eliane Abou-Mansour et Florence Fontaine. « Changes in Plant Metabolism and Accumulation of Fungal Metabolites in Response to Esca Proper and Apoplexy Expression in the Whole Grapevine ». Phytopathology® 106, no 6 (juin 2016) : 541–53. http://dx.doi.org/10.1094/phyto-09-15-0207-r.
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Trunk diseases have become among the most important grapevine diseases worldwide. They are caused by fungal pathogens that attack the permanent woody structure of the vines and cause various symptoms in woody and annual organs. This study examined modifications of plant responses in green stem, cordon, and trunk of grapevines expressing Esca proper (E) or apoplexy (A) event, which are the most frequent grapevine trunk disease symptoms observed in Europe. Transcript expression of a set of plant defense- and stress-related genes was monitored by quantitative reverse-transcription polymerase chain reaction while plant phytoalexins and fungal metabolites were quantified by high-performance liquid chromatography-mass spectrometry in order to characterize the interaction between the grapevine and trunk disease agents. Expression of genes encoding enzymes of the phenylpropanoid pathway and trans-resveratrol content were altered in the three organs of diseased plants, especially in the young tissues of A plants. Pathogenesis-related proteins and the antioxidant system were severely modulated in A plants, which indicates a drastic stress effect. In the meantime, fungal polyketides 6-MSA, (R)-mellein, and (3R,4R)-4-hydroxymellein, were accumulated in A plants, which suggests their potential effect on plant metabolism during the appearance of foliar symptoms.
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Muljukina, N. А., J. Pecenka, R. V. Geretskij et А. Eichmeier. « Grapevine Trunk Diseases Pathogens Identification on Grapevine Rootstocks in Ukraine ». Mikrobiolohichnyi Zhurnal 81, no 2 (30 mars 2019) : 65–72. http://dx.doi.org/10.15407/microbiolj81.02.065.
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MORETTI, Samuele, Andrea PACETTI, Romain PIERRON, Hanns-Heinz KASSEMEYER, Michael FISCHER, Jean-Pierre PÉROS, Gabriel PEREZ-GONZALEZ et al. « Fomitiporia mediterranea M. Fisch., the historical Esca agent : a comprehensive review on the main grapevine wood rot agent in Europe ». Phytopathologia Mediterranea 60, no 2 (13 septembre 2021) : 351–79. http://dx.doi.org/10.36253/phyto-13021.
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Fomitiporia mediterranea M. Fisch. (Fmed) is a basidiomycete first described in 2002, and was considered up to then as part of Fomitiporia punctata (P. Karst) Murrill. This fungus can degrade lignocellulosic biomass, causing white rot and leaving bleached fibrous host residues. In Europe Fmed is considered the main grapevine wood rot (Esca) agent within the Esca disease complex, which includes some of the most economically important Grapevine Trunk Diseases (GTDs). This review summarises and evaluates published research on Fmed, on white rot elimination by curettage or management by treatments with specific products applied to diseased grapevines, and on the relationship between wood symptoms and Grapevine Leaf Stripe Disease (GLSD) in the Esca disease complex. Information is also reviewed on the fungus biology, mechanisms of pathogenicity, and their possible relationships with external foliar symptoms of the Esca disease complex. Information on Fmed control strategies is also reviewed.
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Mundy, D. C., et M. A. Manning. « Physiological response of grapevines to vascular pathogens a review ». New Zealand Plant Protection 64 (8 janvier 2011) : 7–16. http://dx.doi.org/10.30843/nzpp.2011.64.5974.
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The successful infection of a grapevine vascular system by a plant pathogen and expression of disease symptoms occur only when the pathogen has overcome the wound response and other defences of the vine Even when pathogens do successfully infect the vascular system of grapevines symptom expression is not often observed in the first season Symptoms may be observed in one year but the same vine can have reduced or no symptoms the following season Information is presented on physiological stress in association with trunk diseases as one factor that may contribute to symptom expression in vines A hypothesis of grapevine wound response is proposed as part of the discussion of vine physiological response Information on individual trunk diseases and physiological interactions is also provided
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Mundy, D. C., S. R. Haycock, M. A. Manning et A. R. G. McLachlan. « The response to stress treatments of potted grapevines inoculated with Eutypa lata and Botryosphaeria lutea fungi associated with trunk disease ». New Zealand Plant Protection 65 (8 janvier 2012) : 228–35. http://dx.doi.org/10.30843/nzpp.2012.65.5374.
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Current management options for grapevine trunk diseases are focused on ameliorating symptoms once they have occurred In grapevines and other crops plant stress has been observed to increase symptom expression for different pathogens depending on timing and type of stress This research aimed to establish whether stress to vines at the time of wounding enhances colonisation by fungi associated with trunk disease Potted plants were wounded and inoculated with two grapevine trunk fungi Eutypa lata and Botryosphaeria lutea Stress treatments (leaf removal and girdling) were applied to the vines immediately prior to inoculation Lesion size at the wound site and infection confirmed by reisolation of the pathogen were measured In these experiments stress did not induce greater infection incidence or larger lesions at the site of wounding Some difficulties in using potted plants to simulate responses of mature vines in the field are discussed
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Bertsch, C., P. Larignon, S. Farine, C. Clement et F. Fontaine. « The Spread of Grapevine Trunk Disease ». Science 324, no 5928 (7 mai 2009) : 721. http://dx.doi.org/10.1126/science.324_721a.
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Lawrence, Daniel P., Renaud Travadon et Kendra Baumgartner. « Novel Seimatosporium Species from Grapevine in Northern California and Their Interactions with Fungal Pathogens Involved in the Trunk-Disease Complex ». Plant Disease 102, no 6 (juin 2018) : 1081–92. http://dx.doi.org/10.1094/pdis-08-17-1247-re.
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Seimatosporium spp. and closely related “pestalotioid fungi” have been isolated from vineyards worldwide, but their ecological status in grapevine wood is unclear. To determine their involvement in the grapevine trunk-disease complex, we tested the pathogenicity of Californian isolates obtained from vines with general symptoms of Botryosphaeria, Eutypa, and Phomopsis diebacks. Multilocus phylogenetic analyses revealed three species: Seimatosporium vitis and two newly described and typified species, S. luteosporum sp. nov. and S. vitifusiforme sp. nov. Inoculations to woody stems of potted grapevines of both isolates of S. vitis and one isolate of S. vitifusiforme, but not S. luteosporum, were associated with significantly larger lesions than those of noninoculated controls. Coinoculations with trunk pathogens (Cryptovalsa ampelina, Diaporthe ambigua, Diatrypella verruciformis, Diplodia seriata, and Eutypa lata), coisolated from the same wood cankers in the field, brought about increased lesion lengths for S. vitifusiforme paired with D. seriata, and S. luteosporum paired with Diaporthe ambigua. In contrast, there were no differences in lesion lengths of S. vitis and Diatrypella verruciformis or S. vitis and E. lata, inoculated alone or together. Our findings suggest that Seimatosporium spp. are involved in the grapevine trunk-disease complex, and their virulence may depend on or affect that of trunk pathogens.
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DeKrey, David H., Annie E. Klodd, Matthew D. Clark et Robert A. Blanchette. « Grapevine trunk diseases of cold-hardy varieties grown in Northern Midwest vineyards coincide with canker fungi and winter injury ». PLOS ONE 17, no 6 (3 juin 2022) : e0269555. http://dx.doi.org/10.1371/journal.pone.0269555.
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Grapevine trunk diseases make up a disease complex associated with several vascular fungal pathogenic species. Surveys to characterize the composition of grapevine trunk diseases have been conducted for most major grape growing regions of the world. This study presents a similar survey characterizing the fungi associated with grapevine trunk diseases of cold-hardy interspecific hybrid grape varieties grown nearly exclusively in the atypical harsh winter climate of Northern Midwestern United states vineyards. From the 172 samples collected in 2019, 640 isolates obtained by culturing were identified by ITS sequencing and represent 420 sample-unique taxa. From the 420 representative taxa, opportunistic fungi of the order Diaporthales including species of Cytospora and Diaporthe were most frequently identified. Species of Phaeoacremonium, Paraconiothyrium, and Cadophora were also prevalent. In other milder Mediterranean growing climates, species of Xylariales and Botryosphaeriales are often frequently isolated but in this study they were isolated in small numbers. No Phaeomoniellales taxa were isolated. We discuss the possible compounding effects of winter injury, the pathogens isolated, and management strategies. Additionally, difficulties in researching and understanding the grapevine trunk disease complex are discussed.
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Cobos, Rebeca, Rosa María Mateos, José Manuel Álvarez-Pérez, Miguel Angel Olego, Silvia Sevillano, Sandra González-García, Enrique Garzón-Jimeno et Juan José R. Coque. « Effectiveness of Natural Antifungal Compounds in Controlling Infection by Grapevine Trunk Disease Pathogens through Pruning Wounds ». Applied and Environmental Microbiology 81, no 18 (10 juillet 2015) : 6474–83. http://dx.doi.org/10.1128/aem.01818-15.
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ABSTRACTGrapevine trunk fungal pathogens, such asDiplodia seriataandPhaeomoniella chlamydospora, can infect plants through pruning wounds. They cause grapevine trunk diseases and are involved in grapevine decline. Accordingly, the protection of pruning wounds is crucial for the management of grapevine trunk diseases. The efficacy of different natural antifungals in inhibiting the growth of several fungi causing grapevine trunk diseases was evaluatedin vitro. The fungi showing greaterin vitroefficacy were tested on autoclaved grape wood assays againstD. seriataandP. chlamydospora. Based on results from these assays, chitosan oligosaccharide, vanillin, and garlic extract were selected for further evaluation on pruning wounds inoculated withD. seriataandP. chlamydosporain field trials. A significant decrease in plant mortality was observed after 2 years of growth in the plants treated with the different natural antifungals compared to the mortality rate observed in infected plants that were not treated with antifungals. Also, the infection rate for the inoculated pathogens was significantly reduced in plants treated with the selected natural antifungals. Therefore, natural antifungals represent a promising alternative for disease control and could provide significant economic benefits for the grape-growing industry.
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Mundy, D. C., et M. A. Manning. « Ecology and management of grapevine trunk diseases in New Zealand a review ». New Zealand Plant Protection 63 (1 août 2010) : 160–66. http://dx.doi.org/10.30843/nzpp.2010.63.6558.
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Grapevine trunk diseases threaten the longevity of vineyard production in New Zealand This paper provides a summary of the knowledge of the most common fungal grapevine trunk diseases as well as identifying gaps that require further research Current knowledge of symptoms causal organisms etiology and disease control of botryosphaeria dieback esca eutypa dieback and Petri disease are discussed Additional information about how these diseases may be influenced by different vineyard practices common in New Zealand viticulture is provided
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Kraus, Christian, Carolin Rauch, Elisa Maria Kalvelage, Falk Hubertus Behrens, Dagmar d’Aguiar, Cornelia Dubois et Michael Fischer. « Minimal versus Intensive : How the Pruning Intensity Affects Occurrence of Grapevine Leaf Stripe Disease, Wood Integrity, and the Mycobiome in Grapevine Trunks ». Journal of Fungi 8, no 3 (28 février 2022) : 247. http://dx.doi.org/10.3390/jof8030247.
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Previous works on grapevine-trunk diseases indicate that minimal or non-pruning of the grapevine under certain circumstances can significantly reduce the risk of symptom expression. Nevertheless, knowledge of the mechanisms behind these observations are limited. Therefore, it was the aim of this study to investigate in more detail the effect of pruning intensity on the grapevine trunk by means of trunk integrity and the fungal community in the wood tissue. Two German vineyards partially trained in vertical-shoot position and semi-minimally pruned hedges were chosen for this survey due to the accessibility of multi-annual esca-monitoring data. The results revealed that only in one of the two vineyards was the incidence of external esca symptoms significantly reduced over a period of five years (2017–2021) by minimal pruning, which was up to 73.7% compared to intensive pruning. In both vineyards, the trunks of intensively pruned vines not only had more pruning wounds on the trunk (by 86.0% and 72.9%, respectively) than minimally pruned vines, but also exhibited a larger (by 19.3% and 14.7%, respectively) circumference of the trunk head. In addition, the percentage of white rot and necrosis in the trunks of esca-positive and esca-negative vines was analyzed and compared between the two pruning intensities; hereby, significant differences were only found for esca-negative ‘Dornfelder’ vines, in which the proportion of necrosis was higher for intensively pruned vines (23.0%) than for minimally pruned vines (11.5%). The fungal communities of the differently pruned vine trunks were mainly dominated by four genera, which are also associated with GTDs: Diplodia, Eutypa, Fomitiporia and Phaeomoniella. All in all, the fungal diversity and community composition did not differ between minimally and intensively pruned, esca-positive vines.
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Úrbez-Torres, J. R., P. Haag, P. Bowen et D. T. O'Gorman. « Grapevine Trunk Diseases in British Columbia : Incidence and Characterization of the Fungal Pathogens Associated with Black Foot Disease of Grapevine ». Plant Disease 98, no 4 (avril 2014) : 456–68. http://dx.doi.org/10.1094/pdis-05-13-0524-re.
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Black foot disease of grapevines, caused by several fungal species in the genera Campylocarpon, Cylindrocarpon, Cylindrocladiella, and Ilyonectria, causes significant economic losses to the grapevine industry worldwide. This study represents the first attempt to identify and characterize the fungal pathogens associated with black foot disease of grapevines in British Columbia (BC). Field surveys conducted throughout all grape-growing regions in BC that included assessment of foliar symptomatology and isolations from symptomatic vines showed Cylindrocarpon/Ilyonectria spp. occurred in 32 of 90 (35.5%) young vineyards surveyed (≤8 year old) and in 41 of 215 (19%) samples collected. In 20 of the 41 (48.8%) samples, Cylindrocarpon/Ilyonectria spp. were the sole fungi isolated from symptomatic tissue. In the rest of the samples, black foot fungi were found to primarily coexist with fungal taxa associated with Petri disease of grapevines. Colony and conidia phenotypical characterization, along with DNA analyses of the internal transcribed spacer region (ITS1-5.8S-ITS2) of the rDNA, and part of the β-tubulin and translation elongation factor 1-α genes, revealed five different black foot fungi occurring in declining young vines in BC, namely Cylindrocarpon pauciseptatum, Ilyonectria liriodendri, Ilyonectria macrodidyma, Ilyonectria robusta, and Ilyonectria torresensis. Pathogenicity studies showed all five species to be highly virulent in the grapevine rootstock cultivar 3309C. Overall, I. liriodendri and I. macrodidyma were the most virulent species when inoculated in Vitis vinifera ‘Chardonnay’ and rootstock 3309C.
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Mundy, D. C. « Strategic framework for New Zealand grapevine trunk disease ». New Zealand Plant Protection 70 (31 juillet 2017) : 330. http://dx.doi.org/10.30843/nzpp.2017.70.106.
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Grapevine trunk diseases (GTDs) threaten the longevity of vineyards in New Zealand and internationally. Researchers and wine industry representatives met in Christchurch in November 2016 to develop a strategic plan to enhance the progress of GTD research and improve consistency across studies in New Zealand over the next ten years. Four outcome areas were identified: disease prevention and improved sustainability; improved management decisions; fewer susceptible vines; and improved pathogen management in nurseries and young vines. The group used these outcomes to determine four research focus areas: Disease Etiology/Pathology; Biologicals affecting the host or pathogen; Breeding; and Physiology (including a holistic measure of plant health and biochemistry). The focus areas and outcomes were used to develop a framework that takes into account current research or related activities, any challenges or issues involved, future research required (underpinning and applied), and potential for technology transfer. Attendees also summarised recent research activity and identified current knowledge gaps. Researchers agreed to: enhance sharing of methods and isolates; improve comparisons between experimental results; and increase awareness of the current range of activities and skills employed. Publishing the framework on the MWRC and PFR websites will allow other researchers to access, and comment on, current thinking on this topic.
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Carlucci, Antonia, Francesca Cibelli, Francesco Lops et Maria Luisa Raimondo. « Characterization of Botryosphaeriaceae Species as Causal Agents of Trunk Diseases on Grapevines ». Plant Disease 99, no 12 (décembre 2015) : 1678–88. http://dx.doi.org/10.1094/pdis-03-15-0286-re.
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Botryosphaeriaceae spp. have a cosmopolitan distribution and a wide range of plant hosts. Over the last 15 years, worldwide, 21 species of this family have been associated with grapevine trunk diseases that cause cankers and dieback on grapevines. Here, we surveyed vineyards of Vitis vinifera ‘Lambrusco’, ‘Sangiovese’, and ‘Montepulciano’ in three areas of the Foggia province (Cerignola, Foggia, and San Severo) in southern Italy. Wood samples from grapevines showing general decline, dieback, cankers, and wood and foliar discoloration yielded 344 fungal isolates identified as Botryosphaeriaceae spp. A phylogenetic study combining internal transcribed spacer and translation elongation factor 1-α sequences of 60 representative isolates identified nine botryosphaeriaceous species: Botryosphaeria dothidea, Diplodia corticola, D. mutila, D. seriata, Dothiorella iberica, Do. sarmentorum, Lasiodiplodia citricola, L. theobromae, and Neofusicoccum parvum. Pathogenicity tests confirmed that all nine species cause canker and dieback of grapevines. However, this is the first report of L. citricola as causal agent of wood cankers and dieback of grapevine. To date, including L. citricola, there are 25 botryosphaeriaceous species associated with V. vinifera worldwide, of which 12 have been reported for grapevines in Italy.
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Arzanlou, Mahdi, et Abolfazl Narmani. « ITS sequence data and morphology differentiate Cytospora chrysosperma associated with trunk disease of grapevine in northern Iran ». Journal of Plant Protection Research 55, no 2 (1 avril 2015) : 117–25. http://dx.doi.org/10.1515/jppr-2015-0015.
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Abstract Trunk diseases are potential threats for the grapevine industry owing to the worldwide incidence and economic impact of the diseases. Several fungal groups are known to be involved in these diseases. In a survey on grapevine trunk diseases in northern Iran, Cytospora isolates were repeatedly recovered from vines showing decline symptoms. The symptoms appeared as pale brown to brown streaks in longitudinal cuts of shoots. The morphological and cultural characteristics of the isolates were in agreement with the description of Cytospora chrysosperma. Sequence data of the ITS-rDNA region was used to further confirm the identity of the species. Phylogenetic analysis based on the sequence data obtained in this study and the sequences from GenBank, confirmed the morphological identification. Our isolates were clustered together with C. chrysosperma isolates known from other woody host plant species. The pathogenicity assay on detached shoots of grapevines induced the same symptoms as was observed in field conditions. Although, C. chrysosperma is known from several woody hosts in Iran, the occurrence of this species on grapevines showing decline symptoms is new. The economic impact, distribution, and degree of involvement of C. chrysosperma in decline of vines in other regions of Iran remains to be studied.
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Úrbez-Torres, J. R., F. Peduto, R. J. Smith et W. D. Gubler. « Phomopsis Dieback : A Grapevine Trunk Disease Caused by Phomopsis viticola in California ». Plant Disease 97, no 12 (décembre 2013) : 1571–79. http://dx.doi.org/10.1094/pdis-11-12-1072-re.
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Field surveys recently conducted in California and in other grape-growing regions in the United States showed Phomopsis viticola to be one of the most prevalent fungi isolated from grapevine perennial cankers in declining vines. The current study has not only confirmed the presence of P. viticola from grapevine cankers in California but also has for the first time revealed the occurrence of Diaporthe ambigua, D. eres, and D. neotheicola in symptomatic grapevine wood in California by means of morphological studies and multi-gene sequence analysis. Pathogenicity trials conducted on mature cordons of Vitis vinifera ‘Syrah’ and ‘Red Globe’, as well as on lignified Syrah dormant canes, showed P. viticola isolates from California to be capable of causing perennial cankers. Lengths of vascular discoloration caused by P. viticola were similar to those caused by Eutypa lata and several Botryosphaeriaceae spp., which are well-known grapevine trunk disease pathogens. Additionally, a lack of spring growth was commonly observed in dormant canes inoculated with P. viticola spore suspensions in two pathogenicity trials. As part of this study, V. vinifera ‘Cabernet Sauvignon’ and ‘Zinfandel’ wood was shown to be more susceptible to infection by P. viticola than ‘Barbera’, ‘Chardonnay’, ‘Merlot’, and ‘Thompson Seedless’ wood. After more than 40 years overlooking P. viticola as a grapevine wood pathogen, this study provides strong evidence of the role of P. viticola as a canker-causing organism, and suggests its addition to the fungi involved in the grapevine trunk disease complex. Results from this study suggest D. ambigua and D. neotheicola to be saprophytes or weak pathogens on grapevine wood.
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Cholet, Céline, Émilie Bruez, Pascal Lecomte, Audrey Barsacq, Tommaso Martignon, Massimo Giudici, Marco Simonit, Denis Dubourdieu et Laurence Gény. « Plant resilience and physiological modifications induced by curettage of Esca-diseased grapevines ». OENO One 55, no 1 (12 février 2021) : 153–69. http://dx.doi.org/10.20870/oeno-one.2021.55.1.4478.
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The re-emergence of Grapevine Trunk Diseases (GTDs), mainly Esca, has been observed in most of the world’s vineyards during the last two decades. Development of necrosis in grapevine wood, especially white-rot, is typically associated with Esca-diseased plants. One of the different methods being used in attempts to eradicate GTDs is curettage. This old cultural practice, which consists in surgically removing the necrotic wood, specifically white-rot, retaining only the non-necrotic tissue of Esca-diseased grapevine, is used in some European vineyards (Spain, France, Italy, Portugal), and is being increasingly reintroduced since 10 years ago in France. We, therefore, wanted to study the effect of curettage on vigour, fertility and berry quality, and year after year plant recovery. Our study synthetizes a 3-year experiment on Esca-diseased cv. Sauvignon blanc grapevines curetted in a commercial plot in the Bordeaux region. Asymptomatic control grapevines were compared to Esca-diseased grapevines without curettage (with typical foliar symptoms), and with curetted Esca-diseased grapevines (without foliar symptoms). Even if the curetted grapevines recovered lower vigour and fertility than the control plants, their grape berry quality was comparable, unlike for Esca-diseased grapevines. This cultural practice proved particularly effective in helping Esca-symptomatic grapevines to recover asymptomatic after treatment. Over time, curettage induces the resilience of grapevines, allowing them to recuperate their full physiological functioning, thereby compensating for Esca’s detrimental impact on berry quality.
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Singh, Rupesh Kumar, Eliel Ruiz-May, Vishnu D. Rajput, Tatiana Minkina, Rosa Luz Gómez-Peraza, Krishan K. Verma, Mahipal S. Shekhawat, Catia Pinto, Virgilio Falco et Francisco Roberto Quiroz-Figueroa. « Viewpoint of Chitosan Application in Grapevine for Abiotic Stress/Disease Management towards More Resilient Viticulture Practices ». Agriculture 12, no 9 (2 septembre 2022) : 1369. http://dx.doi.org/10.3390/agriculture12091369.
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Chitosan is a biopolymer with various favorable properties (biotic/abiotic stress mitigation, qualitative improvement, bio-fertilizer, bio-stimulant and postharvest management) to meet multiple agricultural objectives. Grapevine is an important crop and has an enormous impact on the world’s economy due to its derived products, notably the different wine styles. In viticulture, chitosan application made significant developments towards higher contents of beneficial metabolites in grape berries as well as stress and postharvest management during recent decades, although the reports are limited. Recent investigations by our group demonstrated chitosan as a potential elicitor molecule at a molecular level and opened the possibility to use chitosan for trunk disease management; moreover, there are not yet any methods to combat trunk diseases in grapevine. The present viewpoint aimed to summarize the different aspects of chitosan application in grapevine in facilitating the development of inclusive and more integrated sanitary viticulture practices in a sustainable manner.
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Kenfaoui, Jihane, Rachid Lahlali, Mohammed Mennani, Nabil Radouane, Khadija Goura, Hajar El Hamss, Lahsen El Ghadraoui et al. « Botryosphaeria Dieback (Lasiodiplodia viticola) : An Imminent Emerging Threat to the Moroccan Vineyards ». Plants 11, no 16 (21 août 2022) : 2167. http://dx.doi.org/10.3390/plants11162167.
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A decline of various grapevines (Vitis vinifera L.) in the province of Doukkala in Morocco was observed in 2021. The causal pathogen was identified as Lasiodiplodia viticola based on morphological characteristics and phylogenetic analysis of the internal transcribed region (ITS), the β-tubulin gene (TUB) and calmodulin (cmdA). Koch’s postulates were confirmed by successful re-isolation of L. viticola from plants inoculated with the pathogen under controlled conditions. The disease was shown to be prevalent in Bni Hilal (71.43%), Laamria (60%), and Boulaouane (40%) districts, but was quasi-absent in Lmechrek. To understand the dominance of L. viticola as one of the grapevine trunk pathogens, effects of temperature (10–40 °C) and pH (pH 3–pH 12) on growth and sporulation were investigated. The species were able to grow in a range of temperatures ranging from 15 to 40°C and showed a higher growth rate at 35 °C. The fungus were also characterized by a broad optimum pH ranging between 3–12. This study is the first report dealing with L. viticola associated with grapevine trunk diseases in Morocco. Additional studies are therefore required to understand the high occurrence of this disease in vineyards, which is likely due to climate changes. A good understanding of this complex disease might help to develop a reliable and sustainable preventive control strategy.
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Arzanlou, Mahdi, Abolfazl Narmani, Somayeh Moshari, Sima Khodaei et Asadollah Babai-Ahari. « Truncatella angustataassociated with grapevine trunk disease in northern Iran ». Archives Of Phytopathology And Plant Protection 46, no 10 (juin 2013) : 1168–81. http://dx.doi.org/10.1080/03235408.2012.761417.
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Alaniz, Sandra, Josep Armengol, José García-Jiménez, Paloma Abad-Campos et Maela León. « A Multiplex PCR System for the Specific Detection of Cylindrocarpon liriodendri, C. macrodidymum, and C. pauciseptatum from Grapevine ». Plant Disease 93, no 8 (août 2009) : 821–25. http://dx.doi.org/10.1094/pdis-93-8-0821.
Texte intégralRésumé :
Cylindrocarpon liriodendri and C. macrodidymum are the causal agents of grapevine black foot disease. Recently, a third species, C. pauciseptatum, has been isolated from roots of grapevine showing decline symptoms. Currently, reliable identification of isolates of these species through phenotypical characteristics has not been possible. The polymerase chain reaction (PCR)-based method developed in this study allows a quick and easy detection of Cylindrocarpon spp. associated with grapevine. Three primer pairs annealing to variable, partly species-specific sites of the internal transcribed spacer regions amplified species-specific PCR fragments of different sizes in C. liriodendri, C. macrodidymum, and C. pauciseptatum in a multiplex assay with DNA obtained with both quick and traditional extraction methods. They did not generate any PCR product in other fungal trunk pathogens or contaminants commonly associated with grapevines. When universal fungal ITS primers were used in a nested multiplex PCR, the three primer pairs also detected C. liriodendri, C. macrodidymum, and C. pauciseptatum in total DNA extracted from roots of inoculated grapevines. The designed methods can be used for the diagnosis of these fungi from pure culture or infected grapevines.
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VV., AA. « Abstracts of oral and poster presentations given at the 12th International Workshop on Grapevine Trunk Diseases, Mikulov, Czech Republic, 10–14 July 2022 ». Phytopathologia Mediterranea 16, no 2 (2 août 2022) : 319–69. http://dx.doi.org/10.36253/phyto-13818.
Texte intégralRésumé :
The 12th International Workshop on Grapevine Trunk Diseases (12th IWGTD) was held in Mikulov, Czech Republic, from 10 to 14 July, 2022. The Workshop was chaired by Dr Aleš Eichmeier and organized by Mendel University in Brno and Svaz Vinařů České Republiky/Union of Winemakers of the Czech Republic. The ICGTD Council meeting took place on 10 July, and that evening the welcome reception was opened by Rostislav Koštial (Mayor of Mikulov, Senator and viticulturist), Dr Martin Chlad (President of Svaz Vinařů České Republiky/Union of Winemakers of the Czech Republic), and Dr Svatopluk Kapounek (Vice-Rector, Mendel University, Brno).The scientific programme was opened by Dr Alena Salašová (Dean, Faculty of Horticulture, Mendel University, Brno). The Workshop was attended by 133 researchers from 25 countries, presenting 61 oral and 49 poster papers. These presentations were in five sessions, including; Pathogen Characterization and Identification, Epidemiology, Plant-pathogen Interactions, Microbial Ecology, and Disease Management in Nurseries and Vineyards. The Disease Management session aimed to provide grape growers with an overview of practical options for trunk disease control. This session included contributions from researchers who have completed field trials on trunk disease management. David Gramaje (Instituto de Ciencias de la Vid y el Vino (ICVV), Logroño, La Rioja, Spain, outlined an evaluation of treatments for protection of grapevine pruning wounds from natural pathogen infections; Francois Halleen (University of Stellenbosch, South Africa) presented detailed results of efficiency of hot water treatments in nurseries; and Josè Úrbez-Torres (Agriculture and Agri-Food Canada SuRDC) outlined research on biological control of Botryosphaeria dieback in grapevines.A field trip (13 July) visited vineyards in the South Moravia. Delegates were presented with an overview of grapevine production in South Moravia, followed by discussions on the main diseases (including GTDs) affecting grapevines in the Czech Republic. Vineyards affected by GTD pathogens were also visited, where the growers outlined their viewpoints. The field trip focused on presenting the current situation of GTDs in South Moravia, and the management strategies being adopted.
Student competitions for best oral and poster presentations included papers presented by 29 postgraduate students. For best posters:1st place went to Catarina Leal (University of Reims Champagne-Ardenne, France, and Polytechnic University of Valencia, Spain), “Evaluation of Trichoderma atroviride SC1 and Bacillus subtilis PTA-271 combination against grapevine trunk diseases pathogens in nursery propagation process”;2nd place went to Marcelo Bustamante (University of California, Davis, United States of America), “Investigating the role of Fusarium spp. in young vine decline in California”;
3rd place went to María Julia Carbone (Universidad de la República, Montevideo, Uruguay), “Interactive effects of Dactylonectria macrodidyma inoculation on the rhizosphere and root microbiome of grapevine”.For best oral presentations:1st place went to Catarina Leal, (University of Reims Champagne-Ardenne, France, and Polytechnic University of Valencia, Spain), “Beneficial effects of Bacillus subtilis PTA-271 and Trichoderma atroviride SC1 against the Botryosphaeria-dieback pathogen Neofusicoccum parvum may vary with grapevine cultivar”;2nd place went to Isidora Silva-Valderama (University of British Columbia, Vancouver, Canada), “Predicting pathogens virulence: linking host breadth and pathogenicity of the Botryosphaeriaceae fungal family in wine grapes (Vitis vinifera)”;3rd place went to Colin Todd (University of California, Riverside, United States of America), “Tracking the fungal pathobiome associated with young grapevine decline in California nurseries”.The 13th IWGTD will be held in Ensenada, Mexico, in 2025.
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Gramaje, David, Aleš Eichmeier, Milan Spetik, María Julia Carbone, Rebeca Bujanda, Jessica Vallance et Patrice Rey. « Exploring the Temporal Dynamics of the Fungal Microbiome in Rootstocks, the Lesser-Known Half of the Grapevine Crop ». Journal of Fungi 8, no 5 (20 avril 2022) : 421. http://dx.doi.org/10.3390/jof8050421.
Texte intégralRésumé :
Rootstocks are the link between the soil and scion in grapevines, can provide tolerance to abiotic and biotic stresses, and regulate yield and grape quality. The vascular system of grapevine rootstocks in nurseries is still an underexplored niche for research, despite its potential for hosting beneficial and pathogenic microorganisms. The purpose of this study was to investigate the changes in the composition of fungal communities in 110 Richter and 41 Berlandieri rootstocks at four stages of the grapevine propagation process. Taxonomic analysis revealed that the fungal community predominantly consisted of phylum Ascomycota in all stages of the propagation process. The alpha-diversity of fungal communities differed among sampling times for both rootstocks, with richness and fungal diversity in the vascular system decreasing through the propagation process. The core microbiome was composed of the genera Cadophora, Cladosporium, Penicillium and Alternaria in both rootstocks, while the pathogenic genus Neofusicoccum was identified as a persistent taxon throughout the propagation process. FUNguild analysis showed that the relative abundance of plant pathogens associated with trunk diseases increased towards the last stage in nurseries. Fungal communities in the vascular system of grapevine rootstocks differed between the different stages of the propagation process in nurseries. Numerous genera associated with potential biocontrol activity and grapevine trunk diseases were identified. Understanding the large diversity of fungi in the rootstock vascular tissue and the interactions between fungal microbiota and grapevine will help to develop sustainable strategies for grapevine protection.
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Kenfaoui, Jihane, Nabil Radouane, Mohammed Mennani, Abdessalem Tahiri, Lahsen El Ghadraoui, Zineb Belabess, Florence Fontaine et al. « A Panoramic View on Grapevine Trunk Diseases Threats : Case of Eutypa Dieback, Botryosphaeria Dieback, and Esca Disease ». Journal of Fungi 8, no 6 (1 juin 2022) : 595. http://dx.doi.org/10.3390/jof8060595.
Texte intégralRésumé :
Grapevine trunk diseases (GTD) are currently one of the most devastating and challenging diseases in viticulture, leading to considerable yield losses and a remarkable decline in grapevine quality. The identification of the causal agents is the cornerstone of an efficient approach to fighting against fungal diseases in a sustainable, non-chemical manner. This review attempts to describe and expose the symptoms of each pathology related to GTD, the modes of transmission, and the harmfulness of recently reported agents. Special attention was given to new diagnostic tests and technologies, grapevine defense mechanisms, molecular mechanisms of endophytes fungal colonization, and management strategies used to control these threats. The present extended review is, therefore, an updated state-of-the-art report on the progress in the management of vineyards.
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Taillis, David, Anthony Pébarthé-Courrouilh, Émilie Lepeltier, Eva Petit, Antonio Palos-Pinto, Julien Gabaston, Jean-Michel Mérillon, Tristan Richard et Stéphanie Cluzet. « A grapevine by-product extract enriched in oligomerised stilbenes to control downy mildews : focus on its modes of action towards <i>Plasmopara viticola</i> ; ». OENO One 56, no 3 (4 juillet 2022) : 55–68. http://dx.doi.org/10.20870/oeno-one.2022.56.3.4911.
Texte intégralRésumé :
Natural extracts, and particularly plant by-product extracts, can represent an eco-friendly alternative to synthetic pesticides to control crop diseases. We produced an extract from a mix of grapevine trunk and roots that contain a high amount of complex stilbenes (grapevine phytoalexins), especially ε- and r-viniferins. This extract conferred protection to grapevines against Plasmopara viticola (the causal agent of downy mildew) by displaying antimicrobial activities towards zoospore mobility and sporulation and by stimulating plant defences. From the perspective of its application in the field, a formulated preparation of the extract was proposed using polysorbate and sophorolipids. The formulated extract reduced the development of various oomycetes impacting grapevine, potato, tomato and melon in semi-controlled conditions. Thus, such grapevine extract constitutes a promising strategy to protect major cultivated plants against downy mildews.
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Moreno-Sanz, P., G. Lucchetta, A. Zanzotto, M. D. Loureiro, B. Suarez et E. Angelini. « Fungi associated to grapevine trunk diseases in young plants in Asturias (Northern Spain) ». Horticultural Science 40, No. 3 (28 août 2013) : 138–44. http://dx.doi.org/10.17221/9/2013-hortsci.
Texte intégralRésumé :
Dark discolorations of the vascular vessels of 3-year-old potted plants of Asturian grapevine cultivar (Northern Spain), were observed during pruning. These symptoms can be associated to fungal trunk diseases that, in the last decades, are affecting young vineyards all over the world. Cross sections from root, trunk and canes of 19 young grapevine plants were analyzed for the presence of pathogenic fungi associated with these diseases. Non-pathogenic fungi were isolated from both asymptomatic and symptomatic samples, showing that dark discolorations, in some cases, were a consequence of abiotic causes. Regarding pathogenic fungi, Cylindrocarpon spp. colonies were the most frequent, isolated mainly from roots and from asymptomatic tissue. Botryosphaeria spp. colonies were mostly isolated from trunk and from sections with dark discolorations generated by pruning. Phaeoacremonium spp. was isolated from all the organs (roots, trunk and canes). Only one colony of Libertella spp. was isolated. These results suggest the need of a standard protocol, combining treatments and management activities, to be performed in nurseries to limit the spread of these diseases.
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CLAVERIE, Marion, Martin NOTARO, Florence FONTAINE et Jacques WERY. « Current knowledge on Grapevine Trunk Diseases with complex etiology : a systemic approach ». Phytopathologia Mediterranea 59, no 1 (16 avril 2020) : 29–53. http://dx.doi.org/10.36253/phyto-11150.
Texte intégralRésumé :
Among all causes of grapevine decline, Grapevine Trunk Diseases (GTDs) are major concerns for grape growers. This paper reviews knowledge and proposes hypotheses on two major GTDs, esca and Botryosphaeria dieback, and assembles a conceptual model. The objective was to collect information into a sequence, from grapevine nursery propagation processes, through foliar symptom expression, to plant death in mature vineyards. Pathogen infection and colonization steps in woody vine tissues, and the hypotheses that have been formulated to explain the outburst of foliar symptoms, are reported and discussed. Factors that could aggravate or repress GTD symptoms and incidence expansion are also addressed. Vine physiology and pathology together could expand understanding of these diseases. Knowledge and hypotheses that need validation are summarized, and a conceptual model is proposed to explain the occurrence of symptoms and the influencing factors. The model could be useful to cope with the complexity of GTDs, and as a starting point for research to unravel knowledge gaps and suggest new disease management strategies.
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Gkikas, Fedon-Ioannis, Alexandros Tako, Danai Gkizi, Christina Lagogianni, Emmanouil A. Markakis et Sotirios E. Tjamos. « Paenibacillus alvei K165 and Fusarium oxysporum F2 : Potential Biocontrol Agents against Phaeomoniella chlamydospora in Grapevines ». Plants 10, no 2 (22 janvier 2021) : 207. http://dx.doi.org/10.3390/plants10020207.
Texte intégralRésumé :
In the last two decades grapevine trunk diseases (GTDs) have emerged as the most significant threat for grapevine sustainability worldwide. The tracheomycotic fungus Phaeomoniella chlamydospora (Pch) is the predominant GTD-associated species and cannot be controlled with available chemicals. In the present study, we evaluated the effectiveness of two microbial strains (Paenibacillus alvei K165 and Fusarium oxysporum F2) against Pch in grapevine. In vitro bioassays, performed in a growth culture medium simulating the xylem environment, indicated that F2 decreased Pch growth and sporulation, whereas K165 did not have any effect on Pch growth. In planta experiments revealed that root-drench and stem-puncture application of K165 and F2 reduced the endophytic relative DNA amount of Pch by 90% and 82%, respectively, compared to controls. However, wood discoloration, the typical symptom of Pch infection, was not reduced in the F2 treated grapevines. Nevertheless, the F2 treated grapevines harbored higher lignin levels compared to mocks, as it was also done by K165. Therefore, F2 and K165 have the potential to be used as biocontrol agents against Pch in grapevines.
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Muntean, Maria-Doinița, Ana-Maria Drăgulinescu, Liliana Lucia Tomoiagă, Maria Comșa, Horia-Silviu Răcoare, Alexandra Doina Sîrbu et Veronica Sanda Chedea. « Fungal Grapevine Trunk Diseases in Romanian Vineyards in the Context of the International Situation ». Pathogens 11, no 9 (2 septembre 2022) : 1006. http://dx.doi.org/10.3390/pathogens11091006.
Texte intégralRésumé :
Vitis vinifera, known as the common grape vine, represents one of the most important fruit crops in the world. Romania is a wine-producing country with a rich and long tradition in viticulture. In the last decade, increasing reports of damage caused by grapevine trunk diseases (GTDs) have raised concerns in all wine producing countries. Up to now, no study was performed regarding the GTDs situation in Romania, an important grapevine grower in Europe. In this study, we aim, after a comprehensive presentation of the fungal GTDs worldwide, to review the scientific information related to these diseases in Romania in order to open a national platform in an international framework. In order to achieve this, we consulted over 500 references from different scientific databases and cited 309 of them. Our review concludes that, in Romania, there is little amount of available literature on this matter. Three out of six fungal GTDs are reported and well documented in all of the Romanian viticultural zones (except for viticultural zone 4). These are Eutypa dieback, Phomopsis dieback, and Esca disease. Of the fungal pathogens considered responsible Eutypa lata, Phomopsis viticola and Stereum hirsutum are the most studied and well documented in Romania. Management measures are quite limited, and they mostly include preventive measures to stop the GTDs spread and the removal of affected grapevines.
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Yan, J. Y., X. H. Li, F. F. Kong, Z. Y. Wang, L. Z. Gong et H. P. He. « Occurrence of Grapevine Trunk Disease Caused by Botryosphaeria rhodina in China ». Plant Disease 95, no 2 (février 2011) : 219. http://dx.doi.org/10.1094/pdis-02-10-0140.
Texte intégralRésumé :
In the early summer of 2009, grapevine (Vitis vinifera), an important fruit crop in China, declined in most of the vineyards in Hunan, Hubei, and Zhejiang provinces. Characteristic symptoms of Botryosphaeria canker were apparent, including trunk cankers (visible in cross-section), leaf drop, shriveling and drying of fruit clusters, and berry rot (1). To identify the causal pathogen, we tested 126 samples by attempting to culture the pathogen from a small piece of tissue from the canker margin between the necrotic and apparently healthy tissue. Plant tissue was surface sterilized by placing it in 75% ethanol for 1 min and rinsed with sterilized water three times before culturing to potato dextrose agar (PDA) at 28°C. Five days later, the cultures were hyphal-tip purified and then single-spore isolates were used for identification. On the basis of colony characteristics in PDA, these colonies were identified as Botryosphaeria spp. (2). They were grayish white, becoming dark brown with age, and pycnidia were formed after incubation for approximately 9 days. Conidia measured 11 to 15 × 22 to 28 μm. A subset of isolates were used for rDNA ITS (internal transcribed spacer) sequence analysis with primers ITS1 and ITS4 (3). PCR products were separated by electrophoresis and bands were purified (Qiagen Plasmid Mini Kit; Qiagen, Valencia, CA) for sequencing (Sunbiotech Company, Beijing). BLAST searches of three ITS sequences (Accession Nos. GU226851, GU226853, and GU226856) had 100% identity to B. rhodina. EF1-α and β-tubulin sequence analysis gave similar results. Koch's postulates were completed in the laboratory on grape shoots inoculated with two isolates of B. rhodina, originally isolated from plants in the field with symptoms of Botryosphaeria canker. Isolates were incubated on PDA at 25°C for 1 week. Inoculations were made on green shoots of V. vinifera cvs. Muscat Hamburg and Crimson Seedless. Five shoots per cultivar were inoculated per isolate by wounding with a 4-mm cork borer (2 mm deep), placing a colonized agar plug on the wound, and wrapping the wound with Parafilm. Controls were mock inoculated with an agar plug from sterile PDA. Inoculated shoots were incubated in the laboratory in the dark under moist conditions for 10 days at 25°C. Inoculated shoots had necrotic cankers after 10 days and B. rhodina was recovered from each canker margin. The results suggest that some grapevines in China with symptoms of Botryosphaeria canker were indeed infected by B. rhodina. To our knowledge, this is the first report of this pathogen on grapevine in China. References: (1) J. Luque et al. Mycologia 97:1111, 2005. (2) J. M. Niekerk et al. Mycologia 96:781, 2004. (3) T. J. White et al. Page 315 in: PCR Protocols: A Guide to Methods and Applications. Academic Press, San Diego, 1990.
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Billar de Almeida, Angela, Jonathan Concas, Maria Doroteia Campos, Patrick Materatski, Carla Varanda, Mariana Patanita, Sergio Murolo, Gianfranco Romanazzi et Maria do Rosário Félix. « Endophytic Fungi as Potential Biological Control Agents against Grapevine Trunk Diseases in Alentejo Region ». Biology 9, no 12 (26 novembre 2020) : 420. http://dx.doi.org/10.3390/biology9120420.
Texte intégralRésumé :
Grapevine trunk diseases (GTDs) are the most widespread fungal diseases, affecting grapevines in all the major growing regions of the world, and their complete eradication is still not possible. Aiming to search alternatives to avoid the spread and high incidence of these diseases, the present work intended to molecularly identify the grapevine endophytic community, the phytopathogenic fungi associated with GTDs in vineyards within the Alentejo region, and to test potential antagonist microorganisms as biological control candidates against GTDs-associated fungi. Grapevine endophytic community showed a wide variety of fungi in GTDs’ asymptomatic and symptomatic plants, nine of them previously described as GTDs-associated fungi. GTDs prevalent fungi identified in symptomatic plants were Diaporthe sp., Neofusicoccum sp., and H. viticola. Almost all these fungi were also detected in asymptomatic plants, which shows the importance of investigating the interactions of fungal communities and confirms the need for early diagnosis of these diseases. Direct inhibition antagonism tests were performed among identified endophytes and GTDs phytopathogenic fungi, and all the endophyte fungi showed potential as biocontrol agents. Our findings suggest that endophytes are promising candidates for their use in biological control due to their antagonistic activity against the mycelia growth of some GTDs-associated fungi.
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Buzón-Durán, Laura, Natalia Langa-Lomba, Vicente González-García, José Casanova-Gascón, Eva Sánchez-Hernández, Jesús Martín-Gil et Pablo Martín Ramos. « Rutin-stevioside and related conjugates for potential control of grapevine trunk diseases ». Phytopathologia Mediterranea 61, no 1 (25 mars 2022) : 65–77. http://dx.doi.org/10.36253/phyto-13108.
Texte intégralRésumé :
Flavonoids and phenolic acids play roles in grapevine defence against pathogens causing grapevine trunk diseases (GTDs). Rutin is a major flavonoid in vegetative organs of the grapevines, and this compound, unlike other flavonoids, is non-toxic and non-oxidizable. Rutin was assayed in vitro and in vivo against two Botryosphaeriaceae taxa. The limited bioavailability of this compound was circumvented by conjugation with stevioside, a glycoside obtained from Stevia rebaudiana. Clear synergistic effects were observed for the stevioside-rutin adduct, resulting in EC50 and EC90 values of 306.0 and 714.9 μg·mL-1 against Neofusicoccum parvum and 241.6 and 457.8 μg·mL-1 against Dothiorella viticola. In greenhouse experiments, moderate inhibition of N. parvum growth and complete inhibition of D. viticola were observed. These inhibitory effects were greater than those of ferulic acid, which has been considered the most effective phenolic acid against GTDs. Conjugation with stevioside provided solubility enhancement of rutin, paving the way to the design of glycopesticides based on rutin-rich plant extracts as promising antifungals against GTDs.
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Kawaguchi, Akira. « Biocontrol of Grapevine Crown Gall Performed Using Allorhizobium vitis Strain ARK-1 ». Applied Microbiology 2, no 4 (19 novembre 2022) : 981–91. http://dx.doi.org/10.3390/applmicrobiol2040075.
Texte intégralRésumé :
Grapevine crown gall (GCG), which is caused by tumorigenic Allorhizobium vitis (=Rhizobium vitis), is the most important bacterial disease in grapevine, and its economic impact on grapevine is very high. When young vines develop GCG, they often die, whereas older vines may show stress and poor growth depending on the severity of GCG, because GCG interferes with the vascular system of the grapevine trunk and prevents nutrient flow, leading to inferior growth and death. Viticultural practices and chemical control designed to inhibit GCG are only partially effective presently; thus, a biocontrol procedure could be a desirable and effective approach for GCG prevention. This article reviews the practical use of biocontrol options for GCG inhibition that involve using nonpathogenic and antagonistic A. vitis strains. In these studies, screening tests of biocontrol agents discovered nonpathogenic A. vitis strains VAR03-1, ARK-1, ARK-2, and ARK-3. After dipping grapevine roots in a suspension of candidate strains prior to planting in the field, treatment using ARK-1 was shown to significantly reduce the number of plants with GCG. A meta-analysis indicated that ARK-1 is very useful for controlling crown gall in various plant species, including grapevine. It was reported that when a mixture of ARK-1 and a tumorigenic strain was examined in grapevines, the expression levels of several virulence genes of the virulent strain were significantly lower. ARK-1 can reduce the pathogen population in grapevines and gall incidence. Moreover, ARK-1 can prime the induction of certain defense genes of grapevine. These results indicate that ARK-1 has a unique biocontrol mechanism and that it is a promising new biocontrol agent to control GCG.
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Grozić, Kristina, Marijan Bubola et Danijela Poljuha. « Symptoms and management of grapevine trunk diseases ». Journal of Central European Agriculture 20, no 3 (2019) : 876–90. http://dx.doi.org/10.5513/jcea01/20.3.1991.
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Patanita, Mariana, André Albuquerque, Maria Doroteia Campos, Patrick Materatski, Carla M. R. Varanda, Joana A. Ribeiro et Maria do Rosário Félix. « Metagenomic Assessment Unravels Fungal Microbiota Associated to Grapevine Trunk Diseases ». Horticulturae 8, no 4 (29 mars 2022) : 288. http://dx.doi.org/10.3390/horticulturae8040288.
Texte intégralRésumé :
Grapevine trunk diseases (GTDs) are among the most important problems that affect the longevity and productivity of vineyards in all the major growing regions of the world. They are slow-progression diseases caused by several wood-inhabiting fungi with similar life cycles and epidemiology. The simultaneous presence of multiple trunk pathogens in a single plant together with the inconsistent GTDs symptoms expression, their isolation in asymptomatic plants, and the absence of effective treatments make these diseases extremely complex to identify and eradicate. Aiming to gain a better knowledge of GTDs and search sustainable alternatives to limit their development, the present work studied the fungal community structure associated with GTDs symptomatic and asymptomatic grapevines, following a metagenomic approach. Two important cultivars from the Alentejo region with different levels of susceptibility to GTDs were selected, namely, ‘Alicante Bouschet’ and ‘Trincadeira’. Deep sequencing of fungal-directed ITS1 amplicon led to the detection of 258 taxa, including 10 fungi previously described as responsible for GTDs. Symptomatic plants exhibited a lower abundance of GTDs-associated fungi, although with significantly higher diversity of those pathogens. Our results demonstrated that trunk diseases symptoms are intensified by a set of multiple GTDs-associated fungi on the same plant. The composition of fungal endophytic communities was significantly different according to the symptomatology and it was not affected by the cultivar. This study opens new perspectives in the study of GTDs-associated fungi and their relation to the symptomatology in grapevines.
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Qiu, Y., S. Savocchia, C. C. Steel et G. J. Ash. « Botryosphaeria dothideaassociated with grapevine trunk disease in south-eastern Australia ». Australasian Plant Pathology 37, no 5 (2008) : 482. http://dx.doi.org/10.1071/ap08045.
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Brown, A. A., D. P. Lawrence et K. Baumgartner. « Role of basidiomycete fungi in the grapevine trunk disease esca ». Plant Pathology 69, no 2 (27 novembre 2019) : 205–20. http://dx.doi.org/10.1111/ppa.13116.
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Yan, J. Y., Y. L. Peng, Y. Xie, X. H. Li, S. W. Yao, M. L. Tang et Z. Y. Wang. « First Report of Grapevine Trunk Disease Caused by Botryosphaeria obtusa in China ». Plant Disease 95, no 5 (mai 2011) : 616. http://dx.doi.org/10.1094/pdis-11-10-0821.
Texte intégralRésumé :
In September 2010, grapevine (Vitis vinifera) trunk diseases were observed in several vineyards of Yantai District in Shandong Provinces and Changli County of Hebei Provinces of China. Characteristic symptoms of Botryosphaeria canker were apparent, including dark brown discoloration on the trunk (visible in cross-section), cob base shriveling, drying of fruit clusters, and berry falling (2). To identify the causal pathogen, culturing of fungi was attempted from 387 small pieces of tissue from the canker margins of 43 diseased plants. Samples were surface disinfected by placing them in 75% ethanol for 1 min and rinsing with sterilized water three times before culturing on potato dextrose agar (PDA) at 28°C for 7 to 10 days. Fungi isolated were single spored to obtain pure cultures. On the basis of colony characteristics on PDA, 18 isolates from the 387 tissue pieces were eventually identified as Botryosphaeria obtusa (1), Most of the other fungi isolated were B. dothidea. B. obtusa colonies were grayish white, becoming dark brown with age, and pycnidia were formed after incubation for approximately 7 days. Conidia measured 8 to 11 × 17 to 26 μm (n= 50). Two isolates were used for rDNA internal transcribed spacer (ITS) sequence analysis with primers ITS1 and ITS4 (3). PCR products were separated by electrophoresis and bands were purified for legation with PMD-18T (Takara Company, Dalian, China) vector for sequencing. BLAST searches of two ITS sequences had 99 to 100% identity to B. obtusa. EF1-α and β-tubulin sequence analysis gave similar results. Koch's postulates were completed in the greenhouse on grape shoots inoculated with two isolates of B. obtusa originally isolated from diseased plants in the field. Inoculations were made on green shoots of V. vinifera cv. Dunkelfelder T. Six shoots were inoculated per isolate by wounding with a 4-mm cork borer (2 mm deep) and placing a colonized agar plug from a 5-day-old culture on the wound and wrapping it with Parafilm. Controls were mock inoculated with an agar plug from sterile PDA. Inoculated shoots were incubated in the dark under moist conditions in the laboratory for 8 to 10 days at 25°C. Inoculated shoots had necrotic cankers after 8 to 10 days and B. obtusa was recovered from each canker margin. The results indicated that some grapevines in China with symptoms of Botryosphaeria canker were infected by B. obtusa. To our knowledge, this is the first report of this pathogen causing trunk disease on grapevine in China. References: (1) A. Taylor et al. Australas. Plant Pathol. 34:187, 2005. (2) J. R. Úrbez-Torres et al. Plant Dis. 92:519, 2008. (3) T. J. White et al. Page 315 in: PCR Protocols: A Guide to Methods and Applications. Academic Press, San Diego, 1990.
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Jayawardena, Ruvishika S., Mei Liu, Sajeewa S. N. Maharachchikumbura, Wei Zhang, QiKai Xing, Kevin D. Hyde, Somrudee Nilthong, XingHong Li et JiYe Yan. « Neopestalotiopsis vitis sp. nov. causing grapevine leaf spot in China ». Phytotaxa 258, no 1 (26 avril 2016) : 63. http://dx.doi.org/10.11646/phytotaxa.258.1.4.
Texte intégralRésumé :
Neopestalotiopsis species are associated with a post harvest fruit rot and trunk disease of grapevines in China. A new species, N. vitis was isolated from the grape leaf spot in Guangxi Province, China. Initially, small circular, slightly sunken, necrotic spots, developed on the leaf surface, mainly at the inter-vein regions. The spots enlarged rapidly turning irregular, necrotic and brittle with age. Phylogenetic species recognition based on analysis of the combined ITS, TUB2 and tef1 sequence data coupled with morphology distinguished N. vitis from all other known species in the genus. This is the first report of a Neopestalotiopsis species causing a leaf spot on grapevine.
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Geiger, Adrienn, Zoltán Karácsony, József Geml et Kálmán Zoltán Váczy. « Mycoparasitism capability and growth inhibition activity of Clonostachys rosea isolates against fungal pathogens of grapevine trunk diseases suggest potential for biocontrol ». PLOS ONE 17, no 9 (6 septembre 2022) : e0273985. http://dx.doi.org/10.1371/journal.pone.0273985.
Texte intégralRésumé :
The present study aimed to examine the capability of Clonostachys rosea isolates as a biological control agent against grapevine trunk diseases pathogens. Five C. rosea and 174 pathogenic fungal strains were isolated from grafted grapevines and subjected to in vitro confrontation tests. Efficient antagonism was observed against Eutypa lata and Phaeomoniella chlamydospora while mycoparasitism was observed to the pathogens of Botryosphaeria dothidea and Diaporthe spp. pathogens in in vitro dual culture assays. The conidia production of the C. rosea isolates were also measured on PDA plates. One isolate (19B/1) with high antagonistic capabilities and efficient conidia production was selected for in planta confrontation tests by mixing its conidia with the soil of Cabernet sauvignon grapevine cuttings artificially infected with B. dothidea, E. lata and P. chlamydospora. The length and/or the incidence of necrotic lesions caused by E. lata and P. chlamydospora at the inoculation point were significantly decreased after a three months incubation in the greenhouse on cuttings planted in soils inoculated with the conidia of strain 19B/1, while symptom incidence and severity were unaffected in the case of the pathogen B. dothidea. Based on the above results, we consider C. rosea a promising biological control agent against some grapevine trunk diseases.
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GUARNACCIA, Vladimiro, Christian KRAUS, Emmanouil MARKAKIS, Artur ALVES, Josep ARMENGOL, Ales EICHMEIER, Stephane COMPANT et David GRAMAJE. « Fungal trunk diseases of fruit trees in Europe : pathogens, spread and future directions ». Phytopathologia Mediterranea 61, no 3 (13 janvier 2023) : 563–99. http://dx.doi.org/10.36253/phyto-14167.
Texte intégralRésumé :
Production from crops of pome, stone fruit, nut, berry fruit, citrus, grapevine, and olive is increasingly threatened by fungal trunk diseases (FTD). These diseases and the consequent production losses are major problems. Many fungi (including Botryosphaeriaceae, Calosphaeriaceae, Diaporthaceae, Diatrypaceae, Nectriaceae, Phaeomoniellaceae, Pleosporaceae, Togniniaceae, Valsaceae) infect host wood, mainly through wounds and subsequent colonization of woody tissues, causing symptoms such as cankers, gummosis, wood rotting, blight and dieback. Propagative plant material, seedlings and fruit play a significant role in pathogen spread. Several abiotic factors (e.g. shifts in cultural practices and climate change) are involved in the disease development. This paper reviews recent literature on FTD of fruit crops, particularly focusing on the European status of pathogen occurrence. Case studies are described related to diseases of apple, citrus, grapevine, berry, nut and stone fruit, and olive trees. Aspects related to epidemiology and the increase in disease incidence along with the future perspectives on the FTD research are also discussed.
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Karácsony, Zoltán, Dániel G. Knapp, Szabina Lengyel, Gábor M. Kovács et Kálmán Zoltán Váczy. « The fungus Kalmusia longispora is able to cause vascular necrosis on Vitis vinifera ». PLOS ONE 16, no 10 (15 octobre 2021) : e0258043. http://dx.doi.org/10.1371/journal.pone.0258043.
Texte intégralRésumé :
Fungal diseases in agronomically important plants such as grapevines result in significantly reduced production, pecuniary losses, and increased use of environmentally damaging chemicals. Beside the well-known diseases, there is an increased interest in wood-colonizing fungal pathogens that infect the woody tissues of grapevines. In 2015, a traditional isolation method was performed on grapevine trunks showing symptoms of trunk diseases in Hungary. One isolate (T15142) was identified as Kalmusia longispora (formerly Dendrothyrium longisporum) according to morphological and phylogenetic analyses. To evaluate the pathogenicity of this fungus on grapevines, artificial infections were carried out under greenhouse and field conditions, including the CBS 824.84 and ex-type CBS 582.83 strains. All isolates could be re-isolated from inoculated plants; however, varying virulence was observed among them in terms of the vascular necrosis caused. The incidence and severity of this symptom seemed to be congruent with the laccase-producing capabilities of the isolates. This is the first report on the ability of Kalmusia longispora to cause symptoms on grapevines, and on its possible dependence on laccase secretion.
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Foglia, Renzo, Lucia Landi et Gianfranco Romanazzi. « Analyses of Xylem Vessel Size on Grapevine Cultivars and Relationship with Incidence of Esca Disease, a Threat to Grape Quality ». Applied Sciences 12, no 3 (23 janvier 2022) : 1177. http://dx.doi.org/10.3390/app12031177.
Texte intégralRésumé :
Esca disease is one of the most important grapevine trunk diseases. It seriously reduces the quality and quantity of grapevine production, and results in a shorter vineyard lifespan. Previous studies have suggested that wide xylem vessel diameter favours development on grapevine of Phaeomoniella chlamydospora, one of the fungi involved in esca, thus affecting disease susceptibility. In this study, cultivars mainly originated from European countries, 27 white-berried and 24 red-berried grapevine cultivars, were grown in the same experimental vineyard and were analysed for xylem vessel sizes (as diameter and frequency) for correlation with esca incidence. In this study, the cultivars showed significant differences in the xylem vessel parameters. However, no relationship was detected between vessel size and esca incidence in the field. Overall, white-berried cultivars showed wider vessel diameters than red-berried cultivars. The relationship between xylem vessel size in the red-berried and white-berried cultivars and incidence of esca symptoms is discussed. We suggest that vessel anatomy profiles can provide useful information for further investigations on grapevine genotype structure–esca incidence relationships.
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Agustí-Brisach, C., M. León, J. García-Jiménez et J. Armengol. « Detection of Grapevine Fungal Trunk Pathogens on Pruning Shears and Evaluation of Their Potential for Spread of Infection ». Plant Disease 99, no 7 (juillet 2015) : 976–81. http://dx.doi.org/10.1094/pdis-12-14-1283-re.
Texte intégralRésumé :
Four vineyards visibly affected by trunk diseases were surveyed at pruning time in 2012 and 2013 in Spain, to determine whether pruning tools are capable of spreading grapevine trunk diseases from vine to vine. In each vineyard, pruning shears were regularly rinsed with sterile water, collecting liquid samples for analysis. Molecular detection of grapevine fungal trunk pathogens (GFTPs) was performed by nested polymerase chain reaction using specific primers to detect Botryosphaeriaceae spp. Eutypa lata, Cadophora luteo-olivacea, Phaeoacremonium spp., and Phaeomoniella chlamydospora. All of these GFTPs, with the exception of E. lata, were detected in samples from the four vineyards, C. luteo-olivacea and Phaeoacremonium spp. being the most prevalent. Co-occurrence of two, three, or four different GFTPs from the same sample were found, the simultaneous detection of C. luteo-olivacea and Phaeoacremonium spp. being the most prevalent. In addition, fungal isolation from liquid samples in semiselective culture medium for C. luteo-olivacea, Phaeoacremonium spp., and P. chlamydospora was also performed but only C. luteo-olivacea was recovered from samples collected in three of four vineyards evaluated. Pruning shears artificially infested with suspensions of conidia or mycelial fragments of C. luteo-olivacea, Diplodia seriata, E. lata, Phaeoacremonium aleophilum, and Phaeomoniella chlamydospora were used to prune 1-year-old grapevine cuttings of ‘110 Richter’ rootstock. Successful fungal reisolation from the cuttings 4 months after pruning confirmed that infested pruning shears were able to infect them through pruning wounds. These results improve knowledge about the epidemiology of GFTPs and demonstrate the potential of inoculum present on pruning shears to infect grapevines.
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Rizzoli, Attilio, Luca Jelmini, Gianni Boris Pezzatti, Mauro Jermini, Olivier Schumpp, Christophe Debonneville, Enrico Marcolin, Patrik Krebs et Marco Conedera. « Impact of the “Flavescence Dorée” Phytoplasma on Xylem Growth and Anatomical Characteristics in Trunks of ‘Chardonnay’ Grapevines (Vitis vinifera) ». Biology 11, no 7 (28 juin 2022) : 978. http://dx.doi.org/10.3390/biology11070978.
Texte intégralRésumé :
Flavescence dorée (FD) is a grapevine disease caused by ‘Candidatus Phytoplasma vitis’ (FDp), which is epidemically transmitted by the Nearctic leafhopper Scaphoideus titanus. In this study, we applied dendrochronological techniques to analyse the response to FDp infections in terms of wood ring widths and anatomical structures of the xylem and phloem tissues of the trunk of the susceptible grapevine cultivar ‘Chardonnay.’ As a rule, grapevines are susceptible to water shortage and reduce their growth in diameter in case of summer drought. In the season of the external expression of FD symptoms, however, the ring width reductions are extreme and supersede any drought-induced effects. In addition, the anatomy of the phloem tissue in the year of the FD symptom expression appears heavily disarranged. Moreover, in the most suffering individuals, the xylem formation remains incomplete and mostly limited to the early wood tissue. In conclusion, even though the FD phytoplasma does not inhabit and replicate inside the xylem tissue, our results confirm existing indirect inhibiting effects on the ring growth and the xylem tissue formation in FDp-infected grapevines.
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Úrbez-Torres, J. R., P. Haag, P. Bowen et D. T. O'Gorman. « Grapevine Trunk Diseases in British Columbia : Incidence and Characterization of the Fungal Pathogens Associated with Esca and Petri Diseases of Grapevine ». Plant Disease 98, no 4 (avril 2014) : 469–82. http://dx.doi.org/10.1094/pdis-05-13-0523-re.
Texte intégralRésumé :
Esca and Petri disease are two economically important grapevine diseases worldwide. This study reports for the first time the occurrence of both diseases on grapevines in British Columbia (BC) and subsequently in Canada. Visual assessment of 55,699 vines in 118 vineyards revealed a low incidence of esca with only 104 (0.2%) vines showing foliar symptoms. Young vine decline (YVD) was observed in 1,910 (7.8%) of 24,487 monitored young vines and in 52 (8%) of 654 young vines used as re-plants in mature vineyards. In 8 of 51 monitored young vineyards, YVD-affected vines ranged between 15 and 55%. Morphological studies along with DNA analyses of the ITS1-5.8S-ITS2, and part of the β-tubulin, actin, and translation elongation factor 1-α gene regions, allowed us to identify Cadophora luteoolivacea, Phaeomoniella chlamydospora, Phaeoacremonium iranianum, Togninia fraxinopennsylvanica, Togninia minima, and the novel species Phaeoacremonium canadense and Phaeoacremonium roseum from esca and Petri disease infected vines in BC. This study includes for the first time the EF1-α DNA marker in Phaeoacremonium spp. delineation. Pathogenicity studies showed all seven fungi to cause vascular symptoms similar to those observed in esca and Petri disease infected vines. Additionally, the “tiger-stripes” foliar symptom of esca was successfully reproduced when healthy potted vines were inoculated with BC isolates of Pa. chlamydospora, Pm. canadense, Pm. iranianum, T. fraxinopennsylvanica, and T. minima.
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Díaz, G. A., D. Prehn, X. Besoain, E. R. Chávez et B. A. Latorre. « Neofusicoccum parvum Associated with Grapevine Trunk Diseases in Chile ». Plant Disease 95, no 8 (août 2011) : 1032. http://dx.doi.org/10.1094/pdis-03-11-0260.
Texte intégralRésumé :
During 2009 and 2010, a survey (n = 520) of diseased grapevines (Vitis vinifera L.) was done in vineyards located in Maipo and Colchagua valleys (33°43′ to 34°36′S) in Chile. Symptoms of trunk diseases (TD) were observed on >10-year-old grapevines and consisted of short internodes, dead spurs and arms, and dieback. In cross sections, diseased arms and trunks exhibited brown, V-shaped cankers of hard consistency. Collected canker samples from cvs. Cabernet Sauvignon, Carménère, Red Globe, Syrah, and Thompson Seedless were surface sterilized in 75% ethanol for 45 s and plated onto potato dextrose agar modified with 0.005% tetracycline, 0.01% streptomycin, and 0.1% Igepal CO-630 (MPDA; Sigma-Aldrich, St. Louis, MO) for 7 days at 20°C. White-to-gray colonies with aerial mycelium growth turned dark gray after 3 to 5 days and tentatively identified as Botryosphaeriaceae. Hyphal tips of these colonies were transferred to MPDA and kept at 20°C with continuous light. After 30 days, colonies developed black, globose pycnidia with unicellular, hyaline, ellipsoidal, densely granulate, externally smooth, and thin-walled conidia that measured (16.3) 19.3 ± 2.3 (25.9) × (5.8) 7.4 ± 0.8 (9.2) μm (n = 20). Morphologically, these isolates were identified as Neofusicoccum parvum (Pennycook & Samuels) Crous, Slippers & A.J.L. Phillips (2). Nucleotide BLAST analysis of the region ITS1-5.8S-ITS2 of rDNA of N. parvum isolates HMUC-104 and HMUC-105 (GenBank Accession Nos. JF273631 and JF273632) were amplified with ITS4 and ITS5 primers and revealed >99% similarity with the sequence of reference isolate (EU833984). Pathogenicity tests were conducted using isolates HMUC-104 and HMUC-105 on 30-day-old Carménère grapevines (n = 8) rooted in vitro by placing a 3- to 5-mm mycelial plug on the surface of the propagation medium. Additionally, detached green shoots (GS) (n = 5) and dormant canes (DC) (n = 6) 15-cm long were inoculated by placing a 3- to 5-mm mycelial plug underneath a cut aseptically made in the cortex. The GS and DC were placed in humidity chambers at 20 and 25°C, respectively. For controls, an equal number of rooted vines, in vitro vines, GS, and DC were treated with sterile agar plugs. Leaf number (LN), shoot length (SL), and root length (RL) were assessed on rooted plants in vitro after 30 days at 20°C. The extent of vascular discoloration (VD) of GS and DC were determined 15 and 45 days, respectively. N. parvum significantly (P < 0.05) reduced the LN, SL, and RL relative to the control plants. The length of VD varied from 54.86 to 55.39 mm and 14.8 to 15.48 mm in inoculated GS and DC, respectively. No VD symptoms were observed on the controls. N. parvum was reisolated from 100% of the inoculated in vitro plants, GS, and DC, completing Koch's postulates. N. parvum has been documented as a canker pathogen on V. vinifera and is known to contribute to the decline of grapevines. To our knowledge, this is the first report of N. parvum causing bot canker on grapevines in Chile, but has previously been reported in Australia, Spain, and the United States. Of 520 diseased plants in this study, 10 to 15% prevalence was estimated for TD and almost 2% prevalence was associated to N. parvum. Other Botryosphaeriaceae spp. were isolated with N. parvum from grapevine TD in Chilean vineyards (1,3,4). References: (1) J. Auger et al. Plant Dis. 88:1286, 2004. (2) P. W. Crous et al. Stud. Mycol. 55:235, 2006. (3) B. A. Latorre et al. Phytopathology 76:1112, 1986. (4) A. Morales et al. Phytopathol. Mediterr. 49:112, 2010.
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Kanetis, Loukas I., Demetris Taliadoros, Georgios Makris et Michalis Christoforou. « A Novel Seimatosporium and Other Sporocadaceae Species Associated with Grapevine Trunk Diseases in Cyprus ». Plants 11, no 20 (16 octobre 2022) : 2733. http://dx.doi.org/10.3390/plants11202733.
Texte intégralRésumé :
Besides well-known grapevine trunk disease (GTD)-related pathogens, there is an increased interest in wood-colonizing fungi that infect grapevines. During 2017–2018, a survey was conducted in Cyprus and wood samples were collected from vines exhibiting typical GTD symptoms. Based on morphological and multilocus phylogenetic analyses (ITS, LSU, bt2, tef1-a), four species in the Sporocadaceae family were described and typified; two in the genus of Seimatosporium: Seim. cyprium sp. nov. and Seim. vitis-viniferae and two in Sporocadus: Spo. kurdistanicus and Spo. rosigena. The teleomorph of Seim. cyprium sp. nov. was also described. Pathogenicity trials with representative isolates of each species were performed on woody stems of two-year-old potted grapevines for 12 months under field conditions. All isolates were pathogenic, causing dark brown to black vascular discoloration, extending upward and downward from the inoculation point. Sporocadus isolates were significantly more aggressive than Seimatosporium with lesion lengths ranging from 9.24 to 6.90 and 4.13 to 4.00 cm, respectively. Successful re-isolations were also evident for all species and isolates. Seim. cyprium sp. nov. is a newly described species, while Spo. kurdistanicus and Spo. rosigena are reported for the first time in Europe on Vitis vinifera, suggesting the potential role of Sporocadaceae in the GTDs complex
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Hillis, Vicken, Mark Lubell, Jonathan Kaplan, David Doll et Kendra Baumgartner. « The Role of Pest Control Advisers in Preventative Management of Grapevine Trunk Diseases ». Phytopathology® 106, no 4 (avril 2016) : 339–47. http://dx.doi.org/10.1094/phyto-10-15-0250-r.
Texte intégralRésumé :
Vineyards with trunk diseases (Botryosphaeria dieback, Esca, Eutypa dieback, and Phomopsis dieback) can have negative returns in the long run. Minimizing economic impacts depends on effective management, but adopting a preventative practice after infection occurs may not improve yields. Pest control advisers may reduce grower uncertainty about the efficacy of and need for prevention, which often entails future and unobservable benefits. Here, we surveyed advisers in California to examine their influence over grower decision-making, in the context of trunk diseases, which significantly limit grape production and for which curative practices are unavailable. Our online survey revealed adviser awareness of high disease incidence, and reduced yields and vineyard lifespan. Advisers rated both preventative and postinfection practices positively. Despite higher cost estimates given to postinfection practices, advisers did not recommend preventative practices at higher rates. High recommendation rates were instead correlated with high disease incidence for both preventative and postinfection practices. Recommendation rates declined with increasing cost for preventative, but not for postinfection, practices. Our findings suggest that even when advisers acknowledge the risks of trunk diseases, they may not recommend preventative practices before infection occurs. This underscores the importance of clear outreach, emphasizing both the need for prevention and its long-term cost efficacy.