Littérature scientifique sur le sujet « Tripartite GFP »
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Articles de revues sur le sujet "Tripartite GFP"
Finnigan, Gregory C., Angela Duvalyan, Elizabeth N. Liao, Aspram Sargsyan et Jeremy Thorner. « Detection of protein–protein interactions at the septin collar inSaccharomyces cerevisiaeusing a tripartite split-GFP system ». Molecular Biology of the Cell 27, no 17 (septembre 2016) : 2708–25. http://dx.doi.org/10.1091/mbc.e16-05-0337.
Texte intégralSicard, Mathieu, Karine Brugirard-Ricaud, Sylvie Pag�s, Anne Lanois, Noel E. Boemare, Michel Breh�lin et Alain Givaudan. « Stages of Infection during the Tripartite Interaction between Xenorhabdus nematophila, Its Nematode Vector, and Insect Hosts ». Applied and Environmental Microbiology 70, no 11 (novembre 2004) : 6473–80. http://dx.doi.org/10.1128/aem.70.11.6473-6480.2004.
Texte intégralPedelacq, Jean-Denis, et Stéphanie Cabantous. « Development and Applications of Superfolder and Split Fluorescent Protein Detection Systems in Biology ». International Journal of Molecular Sciences 20, no 14 (15 juillet 2019) : 3479. http://dx.doi.org/10.3390/ijms20143479.
Texte intégralBarthe, Lucie, Vanessa Soldan et Luis F. Garcia-Alles. « Assessment of oligomerization of bacterial micro-compartment shell components with the tripartite GFP reporter technology ». PLOS ONE 18, no 11 (27 novembre 2023) : e0294760. http://dx.doi.org/10.1371/journal.pone.0294760.
Texte intégralJahns, Anika C., et Bernd H. A. Rehm. « Tolerance of the Ralstonia eutropha Class I Polyhydroxyalkanoate Synthase for Translational Fusions to Its C Terminus Reveals a New Mode of Functional Display ». Applied and Environmental Microbiology 75, no 17 (6 juillet 2009) : 5461–66. http://dx.doi.org/10.1128/aem.01072-09.
Texte intégralKoraïchi, Faten, Rémi Gence, Catherine Bouchenot, Sarah Grosjean, Isabelle Lajoie-Mazenc, Gilles Favre et Stéphanie Cabantous. « High-content tripartite split-GFP cell-based assays to screen for modulators of small GTPase activation ». Journal of Cell Science 131, no 1 (30 novembre 2017) : jcs210419. http://dx.doi.org/10.1242/jcs.210419.
Texte intégralLiu, Tzu‐Yin, Wen‐Chun Chou, Wei‐Yuan Chen, Ching‐Yi Chu, Chen‐Yi Dai et Pei‐Yu Wu. « Detection of membrane protein–protein interaction in planta based on dual‐intein‐coupled tripartite split‐ GFP association ». Plant Journal 94, no 3 (23 mars 2018) : 426–38. http://dx.doi.org/10.1111/tpj.13874.
Texte intégralTurnquist, Emily, Madison N. Schrock, Megan Halloran et Gregory C. Finnigan. « Characterization of Septin Protein Interactions at the Yeast Bud Neck Using a Tripartite Split GFP Detection System ». Microscopy and Microanalysis 24, S1 (août 2018) : 1348–49. http://dx.doi.org/10.1017/s1431927618007225.
Texte intégralAgarwal, Neeraj, Sebastien Rinaldetti, Bassem B. Cheikh, Qiong Zhou, Evan P. Hass, Robert T. Jones, Molishree Joshi et al. « TRIM28 is a transcriptional activator of the mutant TERT promoter in human bladder cancer ». Proceedings of the National Academy of Sciences 118, no 38 (13 septembre 2021) : e2102423118. http://dx.doi.org/10.1073/pnas.2102423118.
Texte intégralHwang, Moonsun, Jae-kyun Ko, Noah Weisleder, Hiroshi Takeshima et Jianjie Ma. « Redox-dependent oligomerization through a leucine zipper motif is essential for MG53-mediated cell membrane repair ». American Journal of Physiology-Cell Physiology 301, no 1 (juillet 2011) : C106—C114. http://dx.doi.org/10.1152/ajpcell.00382.2010.
Texte intégralThèses sur le sujet "Tripartite GFP"
Koraïchi, Faten. « Etude de l'activation de la GTPase RhoB par complémentation split-GFP tripartite ». Thesis, Toulouse 3, 2016. http://www.theses.fr/2016TOU30081.
Texte intégralRhoB is a small GTPase that is rapidly activated in response to growth factors and cellular stress. It regulates fundamental biological processes such as cell migration, angiogenesis, DNA repair, apoptosis and response to anticancer therapies. Small GTPases activity is tightly regulated by their subcellular localization. However, RhoB activation had never been investigated in living cells. In this work, we have adapted and validated an innovative method of protein-protein interactions analysis using tripartite split-GFP complementation, for the sensitive and specific detection of small GTPases activation in living cells. Then, we developed an optimized cellular model by combining the tripartite split-GFP technology with an anti-GFP intrabody fluorescence-enhancer to detect the regulation of RhoB activation with high spatial resolution. This biosensor highlighted the translocation of active RhoB from endosomes to accumulate at the plasma membrane upon serum stimulation, revealing a novel membrane signaling platform of RhoB. Future studies based on this biosensor will enable the analysis of RhoB activation profile and other small GTPases upon various stimuli or in different cellular contexts, as well as the identification of the GTPases partners and activation modulators
Barthe, Lucie. « Les microcompartiments bactériens : étude de l'assemblage des protéines hexamériques des coques et développement d'outils pour les nanotechnologies ». Electronic Thesis or Diss., Université de Toulouse (2023-....), 2024. http://www.theses.fr/2024TLSEI002.
Texte intégralBacterial microcompartments (BMC) are protein structures, naturally found in some bacteria in which they act as bioreactor and process specific substrates. For instance, depending on the BMC type, the enzymatic set they encapsulate can fixate atmospheric CO2 or catabolize the ethanolamine, 1,2-propanediol or the choline. The BMC shell is polyhedral and is composed of 3 different subunits, including the BMC-H, a protomer associating as an hexamer which are the main and the most diverse shell subunits, in terms of number of homologs within a single BMC operon. Indeed, genomic surveys indicate an average of 3,5 BMC-H homologs per operon, with some organisms like Clostridium saccharolyticum WM1 coding for up to 15 BMC-H split between 3 BMC types.Although it has long been thought that only homo-hexamers existed, it was recently evidenced that hetero-hexamer formation occurred between BMC-H homologs in 2 different β-carboxysome-expressing bacteria. Indeed, numerous BMC-H homologs share a high sequence identity, notably at the intra-hexamer interfaces. Besides paving the way for possible hetero-hexamer formation beyond the β-carboxysome, inside organisms equipped with one BMC type, these recent studies raise the question of possible cross-interactions between BMC-H coming from multiple BMC types.One objective during my PhD thesis was to examine the occurrence of hetero-hexamers in nature. To this end, the tripartite GFP was adapted to study protein-protein interactions among BMC-H and implemented on the case study of Klebsiella pneumonia 342 BMC-H. Of note, this organism is very interesting because it has in its genome 3 BMC loci, comprising a total of 11 BMC-H homologs. Then, besides allowing to determine whether hetero-hexamers do form aside from the β-CBX, in 3 other BMC types, their study would also bring some answer elements to the question of the cross-interactions between BMC-H arising from different BMC types.A novel method to enhance a pathway catalytic efficiency (other than by classical enzymatic engineering) is gaining more and more interests nowadays: enzyme spatial organization. The idea is that, by putting in close proximity or in an arranged fashion the enzymes from a metabolic pathway, one could increase the efficiency of the pathway, through substrate channelling between the different enzymes, for instance, or enzyme clusterisation.The majority of hexamers formed by the BMC-H have the intrinsic property to self-assemble and form higher-ordered macrostructures (nanotubes, Swiss-rolls, 2D sheets) when recombinantly expressed alone in E. coli. This peculiarity has already been exploited in multiple studies to create a protein scaffold for the immobilization of enzymes. In these proof-of-concepts, a sole BMC-H was used to build the scaffold, which would only permit to immobilized different enzymes in a random fashion.Here, we propose to go further with the idea of spatial organization and aimed to elaborate a protein platform starting from an hetero-hexamer. This hetero-hexamer would be composed by 2 up to 6 different BMC-H with each BMC-H constituting an anchoring point for a future enzymatic domain. With such platform, the spatial organization of the enzymes would be more finely controlled which would further enhance the catalysis efficiency of a metabolic pathway.To meet this goal, de novo designed BMC-H were created by 2 collaborator teams of computational design. I studied them and searched for BMC-H couples that would depict orthogonal intra-hexamer interfaces. Indeed, to be able to control precisely the organization onto the platform, this would require to ensure a specific BMC-H order within the hetero-hexamer and thus, tightly control which BMC-H is adjacent to which one and prevent any other association
Winner, Katherine M. « A fluorescence-based approach to elucidate the subunit arrangement of the essential tRNA deaminase from Trypanosoma brucei ». Wittenberg University Honors Theses / OhioLINK, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=wuhonors1617803573189193.
Texte intégralTavares, Lucas Alves. « O envolvimento da proteína adaptadora 1 (AP-1) no mecanismo de regulação negativa do receptor CD4 por Nef de HIV-1 ». Universidade de São Paulo, 2016. http://www.teses.usp.br/teses/disponiveis/17/17136/tde-06012017-113215/.
Texte intégralThe Human Immunodeficiency Virus (HIV) is the etiologic agent of Acquired Immunodeficiency Syndrome (AIDS). AIDS is a disease which has a global distribution, and it is estimated that there are currently at least 36.9 million people infected with the virus. During the replication cycle, HIV promotes several changes in the physiology of the host cell to promote their survival and enhance replication. The fast progression of HIV-1 in humans and animal models is closely linked to the function of an accessory protein Nef. Among several actions of Nef, one is the most important is the down-regulation of proteins from the immune response, such as the CD4 receptor. It is known that this action causes CD4 degradation in lysosome, but the molecular mechanisms are still incompletely understood. Nef forms a tripartite complex with the cytosolic tail of the CD4 and adapter protein 2 (AP-2) in clathrin-coated vesicles, inducing CD4 internalization and lysosome degradation. Previous research has demonstrated that CD4 target to lysosomes by Nef involves targeting of this receptor to multivesicular bodies (MVBs) pathway by an atypical mechanism because, although not need charging ubiquitination, depends on the proteins from ESCRTs (Endosomal Sorting Complexes Required for Transport) machinery and the action of Alix, an accessory protein ESCRT machinery. It has been reported that Nef interacts with subunits of AP- 1, AP-2, AP-3 complexes and Nef does not appear to interact with AP-4 and AP-5 subunits. However, the role of Nef interaction with AP-1 or AP-3 in CD4 down-regulation is poorly understood. Furthermore, AP-1, AP-2 and AP-3 are potentially heterogeneous due to the existence of multiple subunits isoforms encoded by different genes. However, there are few studies to demonstrate if the different combinations of APs isoforms are form and if they have distinct functional properties. This study aim to identify and characterize cellular factors involved on CD4 down-modulation induced by Nef from HIV-1. More specifically, this study aimed to characterize the involvement of AP-1 complex in the down-regulation of CD4 by Nef HIV-1 through the functional study of the two isoforms of ?-adaptins, AP-1 subunits. By pull-down technique, we showed that Nef is able to interact with ?2. In addition, our data from immunoblots indicated that ?2- adaptin, not ?1-adaptin, is required in Nef-mediated targeting of CD4 to lysosomes and the ?2 participation in this process is conserved by Nef from different viral strains. Furthermore, by flow cytometry assay, ?2 depletion, but not ?1 depletion, compromises the reduction of surface CD4 levels induced by Nef. Immunofluorescence microscopy analysis also revealed that ?2 depletion impairs the redistribution of CD4 by Nef to juxtanuclear region, resulting in CD4 accumulation in primary endosomes. Knockdown of ?1A, another subunit of AP-1, resulted in decreased cellular levels of ?1 and ?2 and, compromising the efficient CD4 degradation by Nef. Moreover, upon artificially stabilizing ESCRT-I in early endosomes, via overexpression of HRS, internalized CD4 accumulates in enlarged HRS-GFP positive endosomes, where co-localize with ?2. Together, the results indicate that ?2-adaptin is a molecule that is essential for CD4 targeting by Nef to ESCRT/MVB pathway, being an important protein in the endo-lysosomal system. Furthermore, the results indicate that ?-adaptins isoforms not only have different functions, but also seem to compose AP-1 complex with distinct cell functions, and only the AP-1 variant comprising ?2, but not ?1, acts in the CD4 down-regulation induced by Nef. These studies contribute to a better understanding on the molecular mechanisms involved in Nef activities, which may also help to improve the understanding of the HIV pathogenesis and the related syndrome. In addition, this work contributes with the understanding of primordial process regulation on intracellular trafficking of transmembrane proteins.
Chu, Chin-I., et 朱晉毅. « Using Tripartite Split-GFP to Detect Protein-Protein Interaction of Membrane Protein in Plant Cell ». Thesis, 2017. http://ndltd.ncl.edu.tw/handle/wq7z5b.
Texte intégralHsiao, Po-Yuan, et 蕭柏元. « Analysis of protein-protein interaction of the phosphate homeostatsis-related proteins by using tripartite split-GFP complementation assay in planta ». Thesis, 2018. http://ndltd.ncl.edu.tw/handle/7629xd.
Texte intégral國立清華大學
生物資訊與結構生物研究所
106
ABSTRACT Tripartite split-GFP complementation assay is a new protein-protein interaction technique improved from bimolecular fluorescence complementation (BiFC). Tripartite split GFP system is composed of a larger fragment, GFP1-9 (residues 1–193), and two shorter β-strands : GFP10 (residues 194–212) and GFP11 (residues 213–233). There are some disadvantages of BiFC because BiFC is based on bulky fragments that may increase the difficulty of protein folding and interfere with protein function. To test whether tripartite split-GFP is a useful tool in planta, we first chose two proteins involved in the phosphate starvation reponse: the phosphate starvation response 1 (PHR1) and the SPX domain-containing protein 1 (SPX1) proteins. The PHR1 and SPX1 sandwitch proteins (GFP10-PHR1-GFP11 and GFP10-SPX1-GFP11) were used for transient expression in tobacco leaves and localized in the nucleus. Next, we confirmed the previous results that PHR1 interacts with SPX1 using tripartite split-GFP system. This assay showed that PHR1 and SPX1 can form homo-dimer/oligomers itself. We used the Arabidopsis nitrogen limitation adaptation (NLA/BAH1) to verify the specificity of the interaction between PHR1 and SPX1. Our results showed that NLA interacts with both PHR1 and SPX1. In addition, we chose the other PHR1 family protein PHR1-like 3 (PHL3) to test the interaction of PHL3 with PHR1 and SPX1 respectively. The signal strength between PHR1, SPX1 and PHL3 are different. From the strongest to the weakest is PHR1 and PHL3, PHR1 dimer, SPX1 and PHR1, and then the signal of SPX1 and PHL3 was the strongest. Furthermore, we generated and obtained the Arabidopsis transgenic liens overexpessing GFP10-PHR1, SPX1-GFP11 and GFP1–9. Confonal analysis of these lines showed few signals in the root hair of seedlings, indicating that the tripartite spli-GFP system used in transgenic plants of Arabidopsis needs to be improved.
Chapitres de livres sur le sujet "Tripartite GFP"
Pedelacq, Jean-Denis, Geoffrey S. Waldo et Stéphanie Cabantous. « High-Throughput Protein–Protein Interaction Assays Using Tripartite Split-GFP Complementation ». Dans Methods in Molecular Biology, 423–37. New York, NY : Springer New York, 2019. http://dx.doi.org/10.1007/978-1-4939-9624-7_20.
Texte intégralWu, Qiudi, Xiao Wang et Yi-Hui Christine Huang. « Risk Perceptions Following a Substandard Vaccine Crisis in China : An Exploratory Approach to Substantiating the Tripartite Model ». Dans Crisis Management - Principles, Roles and Application [Working Title]. IntechOpen, 2022. http://dx.doi.org/10.5772/intechopen.106986.
Texte intégralMartin, Campbell-Kelly. « Negotiations and mergers, 1961-1963 ». Dans ICL, 206–25. Oxford University PressOxford, 1990. http://dx.doi.org/10.1093/oso/9780198539186.003.0012.
Texte intégralRapports d'organisations sur le sujet "Tripartite GFP"
Ocampo-Gaviria, José Antonio, Roberto Steiner Sampedro, Mauricio Villamizar Villegas, Bibiana Taboada Arango, Jaime Jaramillo Vallejo, Olga Lucia Acosta-Navarro et Leonardo Villar Gómez. Report of the Board of Directors to the Congress of Colombia - March 2023. Banco de la República de Colombia, juin 2023. http://dx.doi.org/10.32468/inf-jun-dir-con-rep-eng.03-2023.
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