Littérature scientifique sur le sujet « Trans Mann »

ZusammenfassungWir stimmen dem in der Leitlinie genannten starken Konsens für Maßnahmen der Reproduktionsmedizin als wichtige Option zur Erfüllung des Kinderwunsches bei trans Personen uneingeschränkt zu. Informationen über diese Maßnahmen müssen Bestandteil von Behandlungen von trans Personen sein. Die geschlechtsangleichende Hormontherapie bei Mann-zu-Frau trans Personen hat jedoch einen negativen Einfluß auf die Spermatogenese. Der Erfolg der Fertiltätsprotektion bei Mann-zu-Frau trans Personen hängt stark von Qualität und Quantität der Spermien bzw. der spermatogonialen Stammzellen ab. Daher sollte die Beratung vor der geschlechtsangleichenden Hormontherapie stattfinden. Zu Beginn sollten eine ausführliche Anamnese, eine körperliche Untersuchung, eine Untersuchung von Serumhormonen (LH, FSH, Testosteron, Östradiol, AMH, Inhibin B) und ggf. eine Untersuchung des Ejakulats erfolgen. Zu den etablierten Methoden der Fertilitätsprotektion zählen Kryokonservierung von Spermien nach Ejakulation, Kryokonservierung von Spermien nach mikrochirurgischer testikulärer Spermienextraktion (mTESE) oder Spermienaspiration aus dem Nebenhoden (MESA) oder Kryokonservierung von spermatogonialen Stammzellen. Letztere sind auch möglich, wenn die geschlechtsangleichende Hormontherapie vor Beginn der Pubertät begonnen wird. Dies ist derzeit noch experimentell. Kryokonservierte Spermien können dazu verwendet werden, im Rahmen einer In-vitro-Fertilisation (IVF) und intrazytoplasmatischen Spermieninjektion (ICSI) mit Frauen eigene Kinder zu zeugen. Leihmutterschaft oder Eizellenspende ist in Deutschland derzeit nicht möglich.Wir wollen betonen, dass das Gespräch über fertilitätsprotektive Maßnahmen so früh wie möglich stattfinden muss, um den Erfolg zu garantieren und Absprachen zwischen den medizinischen Diszplinen zu ermöglichen. Das Ziel sind individuelle Lösungen, die für trans Personen finanzierbar, gangbar und operativ mit einfachen Mitteln zu erreichen sind.

The distribution of beta 1,2 N-acetylglucosaminyltransferase I (NAGT I), alpha 1,3-1,6 mannosidase II (Mann II), beta 1,4 galactosyltransferase (GalT), alpha 2,6 sialyltransferase (SialylT) was determined by immuno-labelling of cryo-sections from HeLa cell lines. Antibody labelling in the HeLa cell line was made possible by stable expression of epitope-tagged forms of these proteins or forms from species to which specific antibodies were available. NAGT I and Mann II had the same distribution occupying the medial and trans cisternae of the stack. GalT and SialylT also had the same distribution but they occupied the trans cisterna and the trans-Golgi network (TGN). These results generalise our earlier observations on the overlapping distribution of Golgi enzymes and show that each of the trans compartments of the Golgi apparatus in HeLa cells contains unique mixtures of those Golgi enzymes involved in the construction of complex, N-linked oligosaccharides.

OBJETIVO: Avaliar o uso do ácido trans, trans-mucônico urinário como biomarcador na monitorização da exposição ocupacional ao benzeno. MÉTODOS: Foi estudado o comportamento do ácido trans, trans-mucônico em amostras de urina de indivíduos expostos (N=36) e não expostos (N=116) ocupacionalmente ao solvente. A concentração urinária do ácido foi determinada por Cromatografia Líquida de Alta Eficiência. A amostra foi constituída de indivíduos expostos ao benzeno em uma refinaria de petróleo localizada em Belo Horizonte, MG. Foram empregados os testes estatísticos não-paramétricos de Kruskall-Wallis, Mann Witney e de correlação de Spearman, ao nível de significância de 0,05%. RESULTADOS: A exposição média ao benzeno dos trabalhadores selecionados foi de 0,15±0,05 mg/m³ (0,05 ppm) o que resultou em um valor médio do metabólito urinário de 0,19±0,04 mg/g de creatinina. A faixa de referência do ácido trans, trans-mucônico no grupo não exposto variou de 0,03 a 0,26 mg/g de creatinina (média de 0,10±0,08 mg/g de creatinina). Foi encontrada uma diferença significativa entre os níveis de ácido trans, trans-mucônico do grupo exposto e não exposto. Entretanto, não houve correlação entre os níveis do metabólito urinário e do benzeno no ar. Foi observada a correlação entre ácido trans, trans-mucônico e hábito de fumar no grupo de indivíduos expostos. A ingestão de álcool num período de até 48 horas antes da coleta das amostras não mostrou interferir nos níveis do metabólito nos dois grupos estudados. Foi observada a correlação entre ácido trans, trans-mucônico e idade (faixa etária de 18 a 25 anos) no grupo de não expostos. CONCLUSÕES: Os resultados obtidos evidenciaram a importância de ser mais bem avaliada a influência do hábito de fumar e da faixa etária do trabalhador nos níveis urinários do ácido trans, trans-mucônico.

Zusammenfassung Einleitung Studien zur Sexualität von trans* Personen sind häufig durch das heteronormative Bias geprägt, alle trans* Personen würden eine Genitalangleichung anstreben, um penil-vaginalen penetrativen Geschlechtsverkehr ausüben zu können, als (trans*) Mann in der insertiven, als (trans*) Frau in der aufnehmenden Rolle. Diese Studie untersucht erstmals die Sexualität von trans* Personen, die keine Genitalangleichung anstreben und mit ihrer Sexualität zufrieden sind. Forschungsziele Ziel der Studie ist es, die individuelle Sexualität von Menschen dieser Personengruppe zu beleuchten und die intrapsychischen, interaktionellen und kollektiven Ressourcen zu analysieren, auf die sie dabei zurückgreifen. Darunter fallen auch Strategien, um den scheinbaren Widerspruch von Geschlechtsidentität und Körperlichkeit zu überbrücken. Methoden Es wurden sechs leitfadengestützte qualitative Interviews durchgeführt und mithilfe einer inhaltlich strukturierenden qualitativen Inhaltsanalyse ausgewertet. Die Studie beinhaltete verschiedene partizipative Elemente. So wurden die Ergebnisse im Rahmen einer partizipativen Feedbackschleife durch die Teilnehmenden kommunikativ validiert. Ergebnisse Die Teilnehmer_innen berichten von vielfältigen, genitalen wie non-genitalen sexuellen Praktiken, die unabhängig von den damit assoziierten Geschlechterrollen stattfinden. Dabei greifen sie auf verschiedene Ressourcen und Strategien zurück. Insbesondere wird die geschlechtsanzeigende Funktion von Genitalien auf drei Ebenen dekonstruiert: individuell durch die Teilnehmenden selbst, interaktionell über die Anerkennung ihrer Geschlechtsidentität durch sexuelle Partner_innen und kollektiv durch die Communitys, in denen sie sich bewegen. Schlussfolgerung Die Studie entkräftet die oft unhinterfragte Vorannahme, dass trans* Personen per se ihre Genitalien nur widerwillig oder unter Erfahrungen von körperlicher Dysphorie in der Sexualität einsetzen. Mit den richtigen Ressourcen müssen sexuelle Rolle, Genitalienstatus und Geschlechtsidentität nicht stereotyp zusammenfallen, um gelingende Sexualität zu praktizieren.

Objective. To verify the relationship between smoking, age, schooling, and the vocal self-perception of trans women. Methods. Cross-sectional observational quantitative study conducted with 24 trans women over 18 years old, living in their affirmed gender for a minimum of 6 months. Data collection involved selected questions from the translated and authorized Portuguese version of the Trans Woman Voice Questionnaire (TWVQ) and information regarding age, education, occupation, and smoking status. All variables were analyzed descriptively, and the association with smoking was assessed using Pearson's Chi-square, Fisher's Exact, Student's T, and Mann-Whitney tests, with a significance level of 5%. Results. The mean age of trans women was 28.2 +/- 6.5 years (range: 21 - 48). Most participants (41.6%) had completed their high school education and pursued diverse careers. Regarding smoking habits, 58.3% of women were either current smokers or had smoked at least 100 cigarettes in their lifetime. A statistically significant association was found between smoking and age (p = 0.001), with smokers having a lower average age (24.9 years) compared to nonsmokers (32.9 years). However, no statistical significance was observed between smoking, education, and self-perception of vocal femininity. Only 9 (37.5%) trans women perceived their voices as feminine, while 17 (70.7%) desired a more feminine-sounding voice. Limitation. The limited sample size in this study may have constrained the ability to detect significant differences between the analyzed groups using statistical tests. Conclusion. The smoking prevalence was notably high among younger trans women. However, there was no statistically significant difference in vocal self-perception between those who smoked and those who did not. The majority of trans women expressed a desire for their voices to sound more feminine, particularly among smokers.

O presente artigo tem por objetivo analisar cinco perfis da rede social Instagram com produção de conteúdos acerca da comunidade não binária. Os perfis utilizados foram: Coletivo Trans Não-Binárie (@coletivotransnaobinarie), Rexistência Não-Binária (@rexistencianaobinaria), Ser Não-Binário (@sernaobinario), Nick Nagari (@nicknagari) e Mar Facciolla (@mardemar.nb); todos eles analisados entre 2021-2022. Essa investigação permite entender como a articulação deste movimento tem criado suas rotas de fuga, subvertendo as armadilhas do CIStema, com o qual rompem, colaborando com o protagonismo trans nas redes. Aqui, debato até que ponto conseguimos cruzar a História com a não binariedade, compreendendo as disputas que há nas narrativas e nos discursos para que construamos histórias não binárias ou uma História da não binariedade no Brasil. Utilizo como aporte teórico: Cristiane Dias (2018), Maria Lugones (2014), Guacira Lopes Louro (2004), Megg Rayara Gomes de Oliveira (2020), Jota Mombaça (2021), Letícia Nascimento (2021), Fernando Seffner (2021), atrelada à Autobixografia (Thallys Mann, 2020) como ferramenta metodológica e minha escrevivência enquanto pessoa não binária para construção das narrativas. Assim, pretendo contribuir ao campo historiográfico dos estudos trans no nosso país.

Le but de cette recherche est de comparer la compréhension sur la connaissance transculturelle dans l’activité de la littératie interculturelle à travers la bande dessinée numérique intitulée "Dari Semarang ke Prancis, Perjalanan Lintas Budaya Indonesia - Prancis" chez les étudiants ayant et n’ayant pas le cours sur l’interculturalité. L'échantillon était composé de 56 étudiants de 10 universités en Indonésie. Le type d'échantillon utilisé est l'échantillonnage en grappes. La collecte de données a été effectuée à l'aide d'un test de la compréhension transculturelle sur quatre épisodes avec 20 questions à choix multiples et vrai-faux chacun via Google Form. En testant les données à l'aide du test de Mann-Whitney qui montre la signature Asymp. Sig. (2-tailed) à signification de 95 % de 0,001. Ainsi, il existe une différence significative dans la compréhension dans la littératie transculturelle chez les étudiants ayant et n’ayant pas le cours interculturel. Et puis, la compréhension dans la littératie transculturelle chez des étudiants ayant le cours interculturel sont meilleure que ceux qui n’ayant pas.
 The aim of this research is to compare the comprehension of trans-cultural literacy between students who get cross-cultural courses and those who don’t, through digital comic entitled "Dari Semarang ke Prancis, Perjalanan Lintas Budaya Indonesia – Prancis”. The sample consisted of 56 students from 10 universities in Indonesia. The type of sample used is cluster sampling. Data collection was carried out using a trans-cultural comprehension test on four episodes with 20 multiple choice and true-false qestions each via Google Form. Testing the data using the Mann-Whitney test which shows Asymp. Sig. (2-tailed) at a significance level of 95% of 0.001, so there is a significant difference in comprehension of trans-cultural literacy for students who get and don’t get cross-cultural courses.

Abstract. Regression analyses were performed to determine the storage conditions that exhibited the best outcomes for long-grain, hybrid milled rice yield and quality. This study evaluated mold population on rough rice, milled rice discoloration, and head rice yield (HRY) after storage of rough rice in airtight conditions at moisture contents (MCs) of 12.5%, 16%, 19%, and 21% wet basis and temperatures of 10°C, 15°C, 20°C, 27°C, and 40°C at two-week intervals for 12 weeks. The experiment used a popular long-grain hybrid rice cultivar (XL745). Rice lots were procured from fields with and without conventional treatment of the field with fungicide for plant disease management. Field treatment and no field treatment were considered as a block, and a Mann-Whitney test was conducted to determine effect. The response surface method, an extension of second-order polynomial regression, was used to examine optimal treatment conditions. Mold population and milled rice discoloration from a combination of storage conditions were predicted using regression models. The first-order and second-order terms of temperature indicated a nonlinear relationship between temperature and ln(discoloration). The MC was positively associated with ln(discoloration), but the degree of impact may change with temperature because the interaction term was significant. From the model evaluation (R2 and lack-of-fit test), the discoloration level is expected to be 57% (49% to 66% confidence interval) under conditions of 20% MC, 40°C, and nine weeks of storage for samples procured from fungicide-treated rice fields. This discoloration change is substantial compared to the initial discoloration of 9%. At high temperature (40°C) and MC (21%), discoloration started immediately after two weeks of storage. Anaerobic storage conditions impeded mold growth, especially at high storage temperature (40°C). Low mold populations were observed in rice stored at low MC (16%). According to the regression model, the critical storage temperature that may lead to discoloration is between 27°C and 40°C. Pre-harvest fungicide treatment of rice in the field for disease control significantly improved the HRY but had no significant influence on mold population or discoloration. This study suggests a range of storage conditions to prevent losses in milling yield and quality of rice. In addition, the studied storage conditions mimicked the typical conditions for on-farm, in-bin drying and storage in the U.S. Mid-South, especially for the top layers of rice inside the bin, and therefore provide an important reference for growers and rice processors using in-bin structures to manage the quality of long-grain hybrid rice. Keywords: Discoloration, Head rice yield, Mold population, Regression analysis, Rice quality, Rice storage.

Abstract An UV/VIS spectrophotometric assay technique was developed that was able to routinely monitor peptidylprolyl cis/trans isomerase (PPIase) activity of biological fluids in 96-well microtiter plates. The assay, based on monitoring the cis-to-trans isomerization of succinyl-Phe-cisPro-Phe-4-nitroanilide as substrate in a chymotrypsin-coupled reaction, yields a throughput of 96 samples per 30 min. The assay’s capacity was exemplified by dealing with the PPIase activity in several normal and pathological human sera. Reference values of 151 healthy subjects (83 females, 69 males, 17 to 60 years old) were found to possess significant sex-specific differences. PPIase activity factor K of the sera was significantly greater in males (5th, 50th, 95th percentiles: 17, 36, 55 K) than females (14, 30, 48 K). PPIase activities of sera from healthy donors (n = 151) were significantly higher (Mann–Whitney rank-sum test P <0.0001) than those of patients (n = 47). PPIase activity in serum samples stored at 4 °C was stable for at least 20 h.

Background Quantitative dynamic contrast enhancement MR imaging (DCE-MRI), used to measure properties of tissue microvasculature and tumor angiogenesis, is a promising method for distinguishing benign and malignant tumors and characterizing tumor response to antiangiogenic treatment. The aim of this study was to assess the feasibility of quantitative parameters derived from clinically used DCE-MRI for distinguishing benign from malignant tumors in the sinonasal area, which may be potentially useful for prediction and monitoring of treatment response to chemoradiotherapy of sinonasal tumors. Methods One hundred and forty-three patients with sinonasal tumors, including 78 malignant tumors and 65 benign tumors and tumor-like lesions, underwent clinically used DCE-MRI. Parametric maps were obtained for quantitative parameters including K trans, kep and v e. Two radiologists reviewed these maps and measured K trans, kep and v e in the tumor tissue. Data were analyzed using independent T-test or Mann-Whitney U test analysis and receiver operating characteristic curves. Results K trans, kep and v e showed significant differences between benign and malignant tumors in the sinonasal area (P=0.000 1). The accuracy of K trans, kep and v e in differentiation between benign and malignant sinonasal tumors were 72.0%, 76.2% and 67.1%, respectively. There were significant differences in kep and v e between malignant epithelial sinonasal tumors and lymphomas (P <0.05). Using a ve value of 0.213 as the threshold value differentiated malignant epithelial tumors from lymphomas with an accuracy of 78.3%, sensitivity of 88.2%, specificity of 68.0%, positive predictive value of 66.7%, and negative predictive value of 90.9%. However, no significant difference in K trans and kep was found between malignant epithelial and non-epithelial tumors in the sinonasal area (P >0.05). Conclusions It is feasible that quantitative parameters of tumors can be derived from clinically used DCE-MRI in the sinonasal region. Preliminary findings suggest an increased value for quantitative DCE-MRI in the evaluation of sinonasal tumors in clinical practice.