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Articles de revues sur le sujet « Synchrotron Radiation Infrared Microspectroscopy »

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Auteur : Grafiati
Publié le 11 mars 2023

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1

Michaelian, Kirk H., Mark D. Frogley, Gianfelice Cinque et Luca Quaroni. « Infrared spectra of micro-structured samples with microPhotoacoustic spectroscopy and synchrotron radiation ». Analyst 145, no 4 (2020) : 1483–90. http://dx.doi.org/10.1039/c9an01281h.

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Qian, Jiang, Xue Gao, Ya-Di Wang, Xue-Ling Li, Jun Hu et Jun-Hong Lü. « Synchrotron Infrared Microspectroscopy for Stem Cell Research ». International Journal of Molecular Sciences 23, no 17 (30 août 2022) : 9878. http://dx.doi.org/10.3390/ijms23179878.

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Stem cells have shown great potential functions for tissue regeneration and repair because of their unlimited self-renewal and differentiation. Stem cells reside in their niches, making them a hotspot for the development and diagnosis of diseases. Complex interactions between niches and stem cells create the balance between differentiation, self-renewal, maturation, and proliferation. However, the multi-facet applications of stem cells have been challenged since the complicated responses of stem cells to biological processes were explored along with the limitations of current systems or methods. Emerging evidence highlights that synchrotron infrared microspectroscopy, known as synchrotron radiation-based Fourier transform infrared microspectroscopy, has been investigated as a potentially attractive technology with its non-invasive and non-biological probes in stem cell research. With their unique vibration bands, the quantitative mapping of the content and distribution of biomolecules can be detected and characterized in cells or tissues. In this review, we focus on the potential applications of synchrotron infrared microspectroscopy for investigating the differentiation and fate determination of stem cells.
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Martínez-Rovira, Immaculada, Olivier Seksek, Josep Puxeu, Joan Gómez, Martin Kreuzer, Tanja Dučić, Maria Josep Ferreres, Manel Artigues et Ibraheem Yousef. « Synchrotron-based infrared microspectroscopy study on the radiosensitization effects of Gd nanoparticles at megavoltage radiation energies ». Analyst 144, no 18 (2019) : 5511–20. http://dx.doi.org/10.1039/c9an00792j.

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Zhu Huachun, 朱化春, 佟亚军 Tong Yajun, 吉特 Ji Te, 彭蔚蔚 Peng Weiwei, 张增艳 Zhang Zengyan, 陈敏 Chen Min et 肖体乔 Xiao Tiqiao. « Spatial Resolution Measurement of Synchrotron Radiation Infrared Microspectroscopy Beamline ». Acta Optica Sinica 35, no 4 (2015) : 0430002. http://dx.doi.org/10.3788/aos201535.0430002.

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Carr, G. L. « Resolution limits for infrared microspectroscopy explored with synchrotron radiation ». Review of Scientific Instruments 72, no 3 (2001) : 1613. http://dx.doi.org/10.1063/1.1347965.

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Rosendahl, Scott M., Ferenc Borondics, Tim E. May, Tor M. Pedersen et Ian J. Burgess. « Interface for time-resolved electrochemical infrared microspectroscopy using synchrotron infrared radiation ». Review of Scientific Instruments 82, no 8 (août 2011) : 083105. http://dx.doi.org/10.1063/1.3624693.

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Ikemoto, Yuka, Manako Tanaka, Tomohiro Higuchi, Toshirou Semba, Taro Moriwaki, Emi Kawasaki et Masayoshi Okuyama. « Infrared Synchrotron Radiation and Its Application to the Analysis of Cultural Heritage ». Condensed Matter 5, no 2 (9 avril 2020) : 28. http://dx.doi.org/10.3390/condmat5020028.

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Infrared synchrotron radiation (IR-SR) is a broad-band light source. Its brilliance is the main advantage for microspectroscopy experiments, when the limited size of the sample often prevents the use of conventional thermal radiation sources. Cultural heritage materials are delicate and valuable; therefore, nondestructive experiments are usually preferred. Nevertheless, sometimes, small pieces can be acquired in the process of preservation and conservation. These samples are analyzed by various experimental techniques and give information about the original material and current condition. In this paper, four attempts to analyze cultural heritage materials are introduced. All these experiments are performed at the microspectroscopy station of IR beamline BL43IR in SPring-8.
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Wetzel, David L., John A. Reffner et Gwyn P. Williams. « Synchrotron infrared microspectroscopy challenges difficult analytical problems ». Proceedings, annual meeting, Electron Microscopy Society of America 54 (11 août 1996) : 262–63. http://dx.doi.org/10.1017/s0424820100163770.

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Micro spectroscopy of molecular species in situ takes advantage of biological concentration by nature of certain molecules within the bulk specimen. Localized hetergeniety is readily documented. Order or randomness may be established for substances that are present in a quantities below the detection limit in the bulk but that may be present in a measurable amount when acute probing is done in the correct region of the specimen. Spectral resolution with a microbeam directed at a relatively small spot from which spectroscopic data is obtained makes this task readily possible. Spectral resolution is achieved with optical efficiency of modern infrared micro spectrometers in which signal is conserved and noise minimized. Improvement of S/N is routinely done by coadding multiple scans however reducing the aperture by half reduces the signal by at least four and doubling S/N requires taking data for four times as long. Thus even if a diffraction limiting situation does not arise one of these desirable goals of 1) good spectral separation (small aperture size), 2) high S/N (good low-noise spectra) and 3) short time of data accumulation must be compromised. This is true with even the most optically efficient instrument however substituting infrared radiation from the vacuum UV storage ring of the National Synchrotron Light Source (Brookhaven National Laboratory) for the conventional thermal (glowbar) instrument source makes it possible to achieve all three goals without the necessity of a severe compromise on at least one of them. The synchrotron beam is 2-3 orders of magnitude brighter, has no thermal noise, and has very low divergence. Aperturing the small concentrated beam results in a relatively small percentage loss in beam intensity.
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Lerch, Ph, P. Dumas, T. Schilcher, A. Nadji, A. Luedeke, N. Hubert, L. Cassinari et al. « Assessing noise sources at synchrotron infrared ports ». Journal of Synchrotron Radiation 19, no 1 (25 novembre 2011) : 1–9. http://dx.doi.org/10.1107/s0909049511041884.

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Today, the vast majority of electron storage rings delivering synchrotron radiation for general user operation offer a dedicated infrared port. There is growing interest expressed by various scientific communities to exploit the mid-IR emission in microspectroscopy, as well as the far infrared (also called THz) range for spectroscopy. Compared with a thermal (laboratory-based source), IR synchrotron radiation sources offer enhanced brilliance of about two to three orders of magnitude in the mid-IR energy range, and enhanced flux and brilliance in the far-IR energy range. Synchrotron radiation also has a unique combination of a broad wavelength band together with a well defined time structure. Thermal sources (globar, mercury filament) have excellent stability. Because the sampling rate of a typical IR Fourier-transform spectroscopy experiment is in the kHz range (depending on the bandwidth of the detector), instabilities of various origins present in synchrotron radiation sources play a crucial role. Noise recordings at two different IR ports located at the Swiss Light Source and SOLEIL (France), under conditions relevant to real experiments, are discussed. The lowest electron beam fluctuations detectable in IR spectra have been quantified and are shown to be much smaller than what is routinely recorded by beam-position monitors.
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Lin Yuecheng, 林乐诚, 佟亚军 Tong Yajun, 吉特 Ji Te, 彭蔚蔚 Peng Weiwei, 肖体乔 Xiao Tiqiao, 朱化春 Zhu Huachun et 陈敏 Chen Min. « Synchrotron Radiation Infrared Three-Dimensional Microspectroscopy Based on Point Scanning Method ». Acta Optica Sinica 40, no 3 (2020) : 0334001. http://dx.doi.org/10.3788/aos202040.0334001.

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Dumas, Paul, Ganesh D. Sockalingum et Josep Sulé-Suso. « Adding synchrotron radiation to infrared microspectroscopy : what's new in biomedical applications ? » Trends in Biotechnology 25, no 1 (janvier 2007) : 40–44. http://dx.doi.org/10.1016/j.tibtech.2006.11.002.

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Sandt, Christophe, Joni Frederick et Paul Dumas. « Profiling pluripotent stem cells and organelles using synchrotron radiation infrared microspectroscopy ». Journal of Biophotonics 6, no 1 (2 novembre 2012) : 60–72. http://dx.doi.org/10.1002/jbio.201200139.

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Dillon, Carolyn Therese. « Synchrotron Radiation Spectroscopic Techniques as Tools for the Medicinal Chemist : Microprobe X-Ray Fluorescence Imaging, X-Ray Absorption Spectroscopy, and Infrared Microspectroscopy ». Australian Journal of Chemistry 65, no 3 (2012) : 204. http://dx.doi.org/10.1071/ch11287.

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This review updates the recent advances and applications of three prominent synchrotron radiation techniques, microprobe X-ray fluorescence spectroscopy/imaging, X-ray absorption spectroscopy, and infrared microspectroscopy, and highlights how these tools are useful to the medicinal chemist. A brief description of the principles of the techniques is given with emphasis on the advantages of using synchrotron radiation-based instrumentation rather than instruments using typical laboratory radiation sources. This review focuses on several recent applications of these techniques to solve inorganic medicinal chemistry problems, focusing on studies of cellular uptake, distribution, and biotransformation of established and potential therapeutic agents. The importance of using these synchrotron-based techniques to assist the development of, or validate the chemistry behind, drug design is discussed.
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Tobin, Mark J., Ljiljana Puskar, Jafar Hasan, Hayden K. Webb, Carol J. Hirschmugl, Michael J. Nasse, Gediminas Gervinskas et al. « High-spatial-resolution mapping of superhydrophobic cicada wing surface chemistry using infrared microspectroscopy and infrared imaging at two synchrotron beamlines ». Journal of Synchrotron Radiation 20, no 3 (22 mars 2013) : 482–89. http://dx.doi.org/10.1107/s0909049513004056.

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The wings of some insects, such as cicadae, have been reported to possess a number of interesting and unusual qualities such as superhydrophobicity, anisotropic wetting and antibacterial properties. Here, the chemical composition of the wings of the Clanger cicada (Psaltoda claripennis) were characterized using infrared (IR) microspectroscopy. In addition, the data generated from two separate synchrotron IR facilities, the Australian Synchrotron Infrared Microspectroscopy beamline (AS-IRM) and the Synchrotron Radiation Center (SRC), University of Wisconsin-Madison, IRENI beamline, were analysed and compared. Characteristic peaks in the IR spectra of the wings were assigned primarily to aliphatic hydrocarbon and amide functionalities, which were considered to be an indication of the presence of waxy and proteinaceous components, respectively, in good agreement with the literature. Chemical distribution maps showed that, while the protein component was homogeneously distributed, a significant degree of heterogeneity was observed in the distribution of the waxy component, which may contribute to the self-cleaning and aerodynamic properties of the cicada wing. When comparing the data generated from the two beamlines, it was determined that the SRC IRENI beamline was capable of producing higher-spatial-resolution distribution images in a shorter time than was achievable at the AS-IRM beamline, but that spectral noise levels per pixel were considerably lower on the AS-IRM beamline, resulting in more favourable data where the detection of weak absorbances is required. The data generated by the two complementary synchrotron IR methods on the chemical composition of cicada wings will be immensely useful in understanding their unusual properties with a view to reproducing their characteristics in, for example, industry applications.
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15

Yu, Peiqiang. « Ultra-spatial synchrotron radiation for imaging molecular chemical structure : Applications in plant and animal studies ». Spectroscopy 21, no 4 (2007) : 183–92. http://dx.doi.org/10.1155/2007/743101.

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Synchrotron-based Fourier transform infrared microspectroscopy (S-FTIR) has been developed as a rapid, direct, non-destructive, bioanalytical technique. This technique takes advantage of synchrotron light brightness and small effective source size and is capable of exploring the molecular chemical features and make-up within microstructures of a biological tissue without destruction of inherent structures at ultra-spatial resolutions within cellular dimension. To date there has been very little application of this advanced synchrotron technique to the study of plant and animal tissues' inherent structure at a cellular or subcellular level. In this article, a novel approach was introduced to show the potential of the newly developed, advanced synchrotron-based analytical technology, which can be used to reveal molecular structural-chemical features of various plant and animal tissues.
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16

Anand, Vijayakumar, Soon Hock Ng, Tomas Katkus, Jovan Maksimovic, Annaleise R. Klein, Jitraporn Vongsvivut, Keith R. Bambery, Mark J. Tobin et Saulius Juodkazis. « Exploiting spatio-spectral aberrations for rapid synchrotron infrared imaging ». Journal of Synchrotron Radiation 28, no 5 (9 août 2021) : 1616–19. http://dx.doi.org/10.1107/s1600577521007104.

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The Infrared Microspectroscopy Beamline at the Australian Synchrotron is equipped with a Fourier transform infrared (FTIR) spectrometer, which is coupled with an infrared (IR) microscope and a choice of two detectors: a single-point narrow-band mercury cadmium telluride (MCT) detector and a 64 × 64 multi-pixel focal plane array (FPA) imaging detector. A scanning-based point-by-point mapping method is commonly used with a tightly focused synchrotron IR beam at the sample plane, using an MCT detector and a matching 36× IR reflecting objective and condenser (NA = 0.5), which is time consuming. In this study, the beam size at the sample plane was increased using a 15× objective and the spatio-spectral aberrations were investigated. A correlation-based semi-synthetic computational optical approach was applied to assess the possibilities of exploiting the aberrations to perform rapid imaging rather than a mapping approach.
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17

Wetzel, David L., John A. Reffner et Gwyn P. Williams. « Ultra-spatially resolved synchrotron powered FT-IR microspectroscopy of individual cells of biological material ». Proceedings, annual meeting, Electron Microscopy Society of America 53 (13 août 1995) : 884–85. http://dx.doi.org/10.1017/s0424820100140798.

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Synchrotron radiation is 100 to 1000 times brighter than a thermal source such as a globar. It is not accompanied with thermal noise and it is highly directional and nondivergent. For these reasons, it is well suited for ultra-spatially resolved FT-IR microspectroscopy. In efforts to attain good spatial resolution in FT-IR microspectroscopy with a thermal source, a considerable fraction of the infrared beam focused onto the specimen is lost when projected remote apertures are used to achieve a small spot size. This is the case because of divergence in the beam from that source. Also the brightness is limited and it is necessary to compromise on the signal-to-noise or to expect a long acquisition time from coadding many scans. A synchrotron powered FT-IR Microspectrometer does not suffer from this effect. Since most of the unaperatured beam’s energy makes it through even a 12 × 12 μm aperture, that is a starting place for aperture dimension reduction.
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Bantignies, J.-L., G. Fuchs, G. L. Carr, G. P. Williams, D. Lutz et S. Marull. « Organic reagent interaction with hair spatially characterized by infrared microspectroscopy using synchrotron radiation ». International Journal of Cosmetic Science 20, no 6 (décembre 1998) : 381–94. http://dx.doi.org/10.1046/j.1467-2494.1998.177055.x.

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Ugawa, A., H. Ishii, K. Yakushi, H. Okamoto, T. Mitani, M. Watanabe, K. Sakai, K. Suzui et S. Kato. « Design of an instrument for far‐infrared microspectroscopy using a synchrotron radiation source ». Review of Scientific Instruments 63, no 1 (janvier 1992) : 1551–54. http://dx.doi.org/10.1063/1.1143017.

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Li, Chao, Ling Zong, Yang Zhong, Mianmian Zhang et Xin Wang. « A study of colorectal cancer patients’ hair medulla by synchrotron radiation infrared microspectroscopy ». Infrared Physics & ; Technology 111 (décembre 2020) : 103569. http://dx.doi.org/10.1016/j.infrared.2020.103569.

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Hahn, F., et C. A. Melendres. « Synchrotron infrared reflectance microspectroscopy study of film formation and breakdown on copper ». Journal of Synchrotron Radiation 17, no 1 (14 novembre 2009) : 81–85. http://dx.doi.org/10.1107/s0909049509040680.

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Zhou, Xiaojie, Jiajia Zhong, Wenjie Yu et Yuzhao Tang. « Synchrotron radiation-based Fourier transform infrared microspectroscopy investigation of WRL68 cells treated with doxorubicin ». Spectrochimica Acta Part A : Molecular and Biomolecular Spectroscopy 283 (décembre 2022) : 121773. http://dx.doi.org/10.1016/j.saa.2022.121773.

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Ikemoto, Yuka, Michio Ishikawa, Satoru Nakashima, Hidekazu Okamura, Yuichi Haruyama, Shinji Matsui, Taro Moriwaki et Toyohiko Kinoshita. « Development of scattering near-field optical microspectroscopy apparatus using an infrared synchrotron radiation source ». Optics Communications 285, no 8 (avril 2012) : 2212–17. http://dx.doi.org/10.1016/j.optcom.2011.12.106.

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Batard, Eric, Frédéric Jamme, Emmanuel Montassier, Dominique Bertrand, Jocelyne Caillon, Gilles Potel et Paul Dumas. « Synchrotron radiation infrared microspectroscopy to assess the activity of vancomycin against endocarditis vegetation bacteria ». Journal of Microbiological Methods 85, no 3 (juin 2011) : 235–38. http://dx.doi.org/10.1016/j.mimet.2011.03.013.

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Chavez-Angel, Emigdio, Ryan C. Ng, Susanne Sandell, Jianying He, Alejandro Castro-Alvarez, Clivia M. Sotomayor Torres et Martin Kreuzer. « Application of Synchrotron Radiation-Based Fourier-Transform Infrared Microspectroscopy for Thermal Imaging of Polymer Thin Films ». Polymers 15, no 3 (19 janvier 2023) : 536. http://dx.doi.org/10.3390/polym15030536.

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The thermal imaging of surfaces with microscale spatial resolution over micro-sized areas remains a challenging and time-consuming task. Surface thermal imaging is a very important characterization tool in mechanical engineering, microelectronics, chemical process engineering, optics, microfluidics, and biochemistry processing, among others. Within the realm of electronic circuits, this technique has significant potential for investigating hot spots, power densities, and monitoring heat distributions in complementary metal–oxide–semiconductor (CMOS) platforms. We present a new technique for remote non-invasive, contactless thermal field mapping using synchrotron radiation-based Fourier-transform infrared microspectroscopy. We demonstrate a spatial resolution better than 10 um over areas on the order of 12 000 um2 measured in a polymeric thin film on top of CaF2 substrates. Thermal images were obtained from infrared spectra of poly(methyl methacrylate) thin films heated with a wire. The temperature dependence of the collected infrared spectra was analyzed via linear regression and machine learning algorithms, namely random forest and k-nearest neighbor algorithms. This approach speeds up signal analysis and allows for the generation of hyperspectral temperature maps. The results here highlight the potential of infrared absorbance to serve as a remote method for the quantitative determination of heat distribution, thermal properties, and the existence of hot spots, with implications in CMOS technologies and other electronic devices.
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Munro, Kristie L., Keith R. Bambery, Elizabeth A. Carter, Ljiljana Puskar, Mark J. Tobin, Bayden R. Wood et Carolyn T. Dillon. « Synchrotron radiation infrared microspectroscopy of arsenic-induced changes to intracellular biomolecules in live leukemia cells ». Vibrational Spectroscopy 53, no 1 (mai 2010) : 39–44. http://dx.doi.org/10.1016/j.vibspec.2010.02.004.

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Delfino, Ines, Valerio Ricciardi et Maria Lepore. « Synchrotron FTIR Microspectroscopy Investigations on Biochemical Changes Occurring in Human Cells Exposed to Proton Beams ». Applied Sciences 12, no 1 (30 décembre 2021) : 336. http://dx.doi.org/10.3390/app12010336.

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Fourier transform infrared microspectroscopy using a synchrotron radiation source (SR-μFTIR) has great potential in the study of the ionizing radiation effects of human cells by analyzing the biochemical changes occurring in cell components. SR-μFTIR spectroscopy has been usefully employed in recent years in some seminal work devoted to shedding light on processes occurring in cells treated by hadron therapy, that is, radiotherapy with charged heavy particles (mainly protons and carbon ions), which is gaining popularity as a cancer treatment modality. These studies are particularly useful for increasing the effectiveness of radiotherapy cancer treatments with charged particles that can offer significant progress in the treatment of deep-seated and/or radioresistant tumors. In this paper, we present a concise revision of these studies together with the basic principles of μFTIR spectroscopy and a brief presentation of the main characteristics of infrared SR sources. From the analysis of the literature regarding the SR-μFTIR spectroscopy investigation on human cells exposed to proton beams, it is clearly shown that changes in DNA, protein, and lipid cell components are evident. In addition, this review points out that the potential offered by SR-μFTIR in investigating the effects induced by charged particle irradiation have not been completely explored. This is a crucial point for the continued improvement of hadron therapy strategies.
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Yu, Peiqiang. « Synchrotron IR microspectroscopy for protein structure analysis : Potential and questions ». Spectroscopy 20, no 5,6 (2006) : 229–51. http://dx.doi.org/10.1155/2006/263634.

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Synchrotron radiation-based Fourier transform infrared microspectroscopy (S-FTIR) has been developed as a rapid, direct, non-destructive, bioanalytical technique. This technique takes advantage of synchrotron light brightness and small effective source size and is capable of exploring the molecular chemical make-up within microstructures of a biological tissue without destruction of inherent structures at ultra-spatial resolutions within cellular dimension. To date there has been very little application of this advanced technique to the study of pure protein inherent structure at a cellular level in biological tissues. In this review, a novel approach was introduced to show the potential of the newly developed, advanced synchrotron-based analytical technology, which can be used to localize relatively “pure“ protein in the plant tissues and relatively reveal protein inherent structure and protein molecular chemical make-up within intact tissue at cellular and subcellular levels. Several complex protein IR spectra data analytical techniques (Gaussian and Lorentzian multi-component peak modeling, univariate and multivariate analysis, principal component analysis (PCA), and hierarchical cluster analysis (CLA) are employed to relatively reveal features of protein inherent structure and distinguish protein inherent structure differences between varieties/species and treatments in plant tissues. By using a multi-peak modeling procedure, RELATIVE estimates (but not EXACT determinations) for protein secondary structure analysis can be made for comparison purpose. The issues of pro- and anti-multi-peaking modeling/fitting procedure for relative estimation of protein structure were discussed. By using the PCA and CLA analyses, the plant molecular structure can be qualitatively separate one group from another, statistically, even though the spectral assignments are not known. The synchrotron-based technology provides a new approach for protein structure research in biological tissues at ultraspatial resolutions.
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Vaccari, L., G. Birada, G. Grenci, S. Pacor et L. Businaro. « Synchrotron radiation infrared microspectroscopy of single living cells in microfluidic devices : advantages, disadvantages and future perspectives ». Journal of Physics : Conference Series 359 (4 mai 2012) : 012007. http://dx.doi.org/10.1088/1742-6596/359/1/012007.

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Szczerbowska-Boruchowska, Magdalena, Paul Dumas, Marzena Zofia Kastyak, Joanna Chwiej, Marek Lankosz, Dariusz Adamek et Anna Krygowska-Wajs. « Biomolecular investigation of human substantia nigra in Parkinson’s disease by synchrotron radiation Fourier transform infrared microspectroscopy ». Archives of Biochemistry and Biophysics 459, no 2 (mars 2007) : 241–48. http://dx.doi.org/10.1016/j.abb.2006.12.027.

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Peng, Shao-En, Chii-Shiarng Chen, Yen-Fang Song, Huai-Ting Huang, Pei-Luen Jiang, Wan-Nan U. Chen, Lee-Shing Fang et Yao-Chang Lee. « Assessment of metabolic modulation in free-living versus endosymbiotic Symbiodinium using synchrotron radiation-based infrared microspectroscopy ». Biology Letters 8, no 3 (16 novembre 2011) : 434–37. http://dx.doi.org/10.1098/rsbl.2011.0893.

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The endosymbiotic relationship between coral hosts and dinoflagellates of the genus Symbiodinium is critical for the growth and productivity of coral reef ecosystems. Here, synchrotron radiation-based infrared microspectroscopy was applied to examine metabolite concentration differences between endosymbiotic (within the anemone Aiptasia pulchella ) and free-living Symbiodinium over the light–dark cycle. Significant differences in levels of lipids, nitrogenous compounds, polysaccharides and putative cell wall components were documented. Compared with free-living Symbiodinium , total lipids, unsaturated lipids and polysaccharides were relatively enriched in endosymbiotic Symbiodinium during both light and dark photoperiods. Concentrations of cell wall-related metabolites did not vary temporally in endosymbiotic samples; in contrast, the concentrations of these metabolites increased dramatically during the dark photoperiod in free-living samples, possibly reflecting rhythmic cell-wall synthesis related to light-driven cell proliferation. The level of nitrogenous compounds in endosymbiotic cells did not vary greatly across the light–dark cycle and in general was significantly lower than that observed in free-living samples collected during the light. Collectively, these data suggest that nitrogen limitation is a factor that the host cell exploits to induce the biosynthesis of lipids and polysaccharides in endosymbiotic Symbiodinium .
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Wu, Qipeng, Chao Li, Zeming Qi, Ling Zong, Chuansheng Hu, Jiarong Li et Xin Wang. « Comparison of hair medulla from esophageal cancer patients and healthy persons using synchrotron radiation infrared microspectroscopy ». Infrared Physics & ; Technology 105 (mars 2020) : 103201. http://dx.doi.org/10.1016/j.infrared.2020.103201.

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Xin, Hangshu, Xuewei Zhang et Peiqiang Yu. « Using Synchrotron Radiation-Based Infrared Microspectroscopy to Reveal Microchemical Structure Characterization : Frost Damaged Wheat vs. Normal Wheat ». International Journal of Molecular Sciences 14, no 8 (14 août 2013) : 16706–18. http://dx.doi.org/10.3390/ijms140816706.

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Fogarty, Simon W., Imran I. Patel, Júlio Trevisan, Takahiro Nakamura, Carol J. Hirschmugl, Nigel J. Fullwood et Francis L. Martin. « Sub-cellular spectrochemical imaging of isolated human corneal cells employing synchrotron radiation-based Fourier-transform infrared microspectroscopy ». Analyst 138, no 1 (2013) : 240–48. http://dx.doi.org/10.1039/c2an36197c.

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Sackett, O., L. Armand, J. Beardall, R. Hill, M. Doblin, C. Connelly, J. Howes, B. Stuart, P. Ralph et P. Heraud. « Taxon-specific responses of Southern Ocean diatoms to Fe enrichment revealed by synchrotron radiation FTIR microspectroscopy ». Biogeosciences 11, no 20 (20 octobre 2014) : 5795–808. http://dx.doi.org/10.5194/bg-11-5795-2014.

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Abstract. Photosynthesis by marine diatoms contributes substantially to global biogeochemical cycling and ecosystem productivity. It is widely accepted that diatoms are extremely sensitive to changes in Fe availability, with numerous in situ experiments demonstrating rapid growth and increased export of elements (e.g. C, Si and Fe) from surface waters as a result of Fe addition. Less is known about the effects of Fe enrichment on the phenotypes of diatoms, such as associated changes in nutritional value – furthermore, data on taxon-specific responses are almost non-existent. Enhanced supply of nutrient-rich waters along the coast of the subantarctic Kerguelen Island provide a valuable opportunity to examine the responses of phytoplankton to natural Fe enrichment. Here we demonstrate the use of synchrotron radiation Fourier Transform Infrared (SR-FTIR) microspectroscopy to analyse changes in the macromolecular composition of diatoms collected along the coast and plateau of Kerguelen Island, Southern Ocean. SR-FTIR microspectroscopy enabled the analysis of individual diatom cells from mixed communities of field-collected samples, thereby providing insight into in situ taxon-specific responses in relation to changes in Fe availability. Phenotypic responses were taxon-specific in terms of intraspecific variability and changes in proteins, amino acids, phosphorylated molecules, silicate/silicic acid and carbohydrates. In contrast to some previous studies, silicate/silicic acid levels increased under Fe enrichment, in conjunction with increases in carbohydrate stores. The highly abundant taxon Fragilariopsis kerguelensis displayed a higher level of phenotypic plasticity than Pseudo-nitzschia spp., while analysis of the data pooled across all measured taxa showed different patterns in macromolecular composition compared to those for individual taxon. This study demonstrates that taxon-specific responses to Fe enrichment may not always be accurately reflected by bulk community measurements, highlighting the need for further research into taxon-specific phenotypic responses of phytoplankton to environmental change.
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Sackett, O., L. Armand, J. Beardall, R. Hill, M. Doblin, C. Connelly, J. Howes, B. Stuart, P. Ralph et P. Heraud. « Taxon-specific responses of Southern Ocean diatoms to Fe enrichment revealed by synchrotron radiation FTIR microspectroscopy ». Biogeosciences Discussions 11, no 5 (21 mai 2014) : 7327–57. http://dx.doi.org/10.5194/bgd-11-7327-2014.

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Abstract. Photosynthesis by marine diatoms contributes substantially to global biogeochemical cycling and ecosystem productivity. It is widely accepted that diatoms are extremely sensitive to changes in Fe availability, with numerous in situ experiments demonstrating rapid growth and increased export of elements (e.g. C, Si and Fe) from surface waters as a result of Fe addition. Less is known about the effects of Fe enrichment on the phenotypes of diatoms, such as associated changes in nutritional value, furthermore data on taxon-specific responses is almost non-existent. Enhanced supply of nutrient-rich waters along the coast of the subantarctic Kerguelen Island provide a valuable opportunity to examine the responses of phytoplankton to natural Fe enrichment. Here we demonstrate the use of synchrotron radiation Fourier Transform Infrared (SR-FTIR) microspectroscopy to analyse changes in the macromolecular composition of diatoms collected along the coast and plateau of Kerguelen Island, Southern Ocean. SR-FTIR microspectroscopy enabled the analysis of individual diatom cells from mixed communities of field-collected samples, thereby providing insight into in situ taxon-specific responses in relation to changes in Fe availability. Phenotypic responses were taxon-specific in terms of intraspecific variability and changes in proteins, amino acids, phosphorylated molecules, silicate and carbohydrates. In contrast to some previous studies, silicate levels increased under Fe enrichment, in conjunction with increases in carbohydrate stores. The highly abundant taxon Fragilariopsis kerguelensis displayed a higher level of phenotypic plasticity than Pseudo-nitzschia spp., while analysis of the data pooled across all measured taxa showed different patterns in macromolecular composition compared to those for individual taxon. This study demonstrates that taxon-specific responses to Fe enrichment may not always be accurately reflected by bulk community measurements, highlighting the need for further research into taxon-specific phenotypic responses of phytoplankton to environmental change.
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Estève, Emmanuel, David Buob, Frédéric Jamme, Chantal Jouanneau, Slavka Kascakova, Jean-Philippe Haymann, Emmanuel Letavernier et al. « Detection and localization of calcium oxalate in kidney using synchrotron deep ultraviolet fluorescence microscopy ». Journal of Synchrotron Radiation 29, no 1 (1 janvier 2022) : 214–23. http://dx.doi.org/10.1107/s1600577521011371.

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Renal oxalosis is a rare cause of renal failure whose diagnosis can be challenging. Synchrotron deep ultraviolet (UV) fluorescence was assayed to improve oxalosis detection on kidney biopsies spatial resolution and sensitivity compared with the Fourier transform infrared microspectroscopy gold standard. The fluorescence spectrum of synthetic mono-, di- and tri-hydrated calcium oxalate was investigated using a microspectrometer coupled to the synchrotron UV beamline DISCO, Synchrotron SOLEIL, France. The obtained spectra were used to detect oxalocalcic crystals in a case control study of 42 human kidney biopsies including 19 renal oxalosis due to primary (PHO, n = 11) and secondary hyperoxaluria (SHO, n = 8), seven samples from PHO patients who received combined kidney and liver transplants, and 16 controls. For all oxalocalcic hydrates samples, a fluorescence signal is detected at 420 nm. These spectra were used to identify standard oxalocalcic crystals in patients with PHO or SHO. They also revealed micrometric crystallites as well as non-aggregated oxalate accumulation in tubular cells. A nine-points histological score was established for the diagnosis of renal oxalosis with 100% specificity (76–100) and a 73% sensitivity (43–90). Oxalate tubular accumulation and higher histological score were correlated to lower estimated glomerular filtration rate and higher urinary oxalate over creatinine ratio.
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Vannocci, Tommaso, Luca Quaroni, Antonio de Riso, Giulia Milordini, Magda Wolna, Gianfelice Cinque et Annalisa Pastore. « Label-Free, Real-Time Measurement of Metabolism of Adherent and Suspended Single Cells by In-Cell Fourier Transform Infrared Microspectroscopy ». International Journal of Molecular Sciences 22, no 19 (4 octobre 2021) : 10742. http://dx.doi.org/10.3390/ijms221910742.

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We used infrared (IR) microscopy to monitor in real-time the metabolic turnover of individual mammalian cells in morphologically different states. By relying on the intrinsic absorption of mid-IR light by molecular components, we could discriminate the metabolism of adherent cells as compared to suspended cells. We identified major biochemical differences between the two cellular states, whereby only adherent cells appeared to rely heavily on glycolytic turnover and lactic fermentation. We also report spectroscopic variations that appear as spectral oscillations in the IR domain, observed only when using synchrotron infrared radiation. We propose that this effect could be used as a reporter of the cellular conditions. Our results are instrumental in establishing IR microscopy as a label-free method for real-time metabolic studies of individual cells in different morphological states, and in more complex cellular ensembles.
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Kawasaki, Takayasu, Heishun Zen, Takeshi Sakai, Yoske Sumitomo, Kyoko Nogami, Ken Hayakawa, Toyonari Yaji, Toshiaki Ohta, Takashi Nagata et Yasushi Hayakawa. « Degradation of Lignin by Infrared Free Electron Laser ». Polymers 14, no 12 (14 juin 2022) : 2401. http://dx.doi.org/10.3390/polym14122401.

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Lignin monomers have attracted attention as functional materials for various industrial uses. However, it is challenging to obtain these monomers by degrading polymerized lignin due to the rigid ether linkage between the aromatic rings. Here, we propose a novel approach based on molecular vibrational excitation using infrared free electron laser (IR-FEL) for the degradation of lignin. The IR-FEL is an accelerator-based pico-second pulse laser, and commercially available powdered lignin was irradiated by the IR-FEL under atmospheric conditions. Synchrotron-radiation infrared microspectroscopy analysis showed that the absorption intensities at 1050 cm−1, 1140 cm−1, and 3400 cm−1 were largely decreased alongside decolorization. Electrospray ionization mass chromatography analysis showed that coumaryl alcohol was more abundant and a mass peak corresponding to hydrated coniferyl alcohol was detected after irradiation at 2.9 μm (νO-H) compared to the original lignin. Interestingly, a mass peak corresponding to vanillic acid appeared after irradiation at 7.1 μm (νC=C and νC-C), which was supported by our two-dimensional nuclear magnetic resonance spectroscopy analysis. Therefore, it seems that partial depolymerization of lignin can be induced by IR-FEL irradiation in a wavelength-dependent manner.
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Lin, Yuecheng, Huachun Zhu, Weiwei Peng, Yao Lu, Yanling Xue, Te Ji et Min Chen. « Three dimensional microspectroscopic tomography with synchrotron radiation infrared raster scanning method ». Infrared Physics & ; Technology 114 (mai 2021) : 103649. http://dx.doi.org/10.1016/j.infrared.2021.103649.

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Josifovska, Natasha, Sofija Andjelic, Lyubomyr Lytvynchuk, Xhevat Lumi, Tanja Dučić et Goran Petrovski. « Biomacromolecular Profile in Human Primary Retinal Pigment Epithelial Cells—A Study of Oxidative Stress and Autophagy by Synchrotron-Based FTIR Microspectroscopy ». Biomedicines 11, no 2 (21 janvier 2023) : 300. http://dx.doi.org/10.3390/biomedicines11020300.

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Synchrotron radiation-based Fourier Transform Infrared (SR-FTIR) microspectroscopy is a non-destructive and chemically sensitive technique for the rapid detection of changes in the different components of the cell’s biomacromolecular profile. Reactive oxygen species and oxidative stress may cause damage to the DNA, RNA, and proteins in the retinal pigment epithelium (RPE), which can further lead to age-related macular degeneration (AMD) and visual loss in the elderly. In this study, human primary RPEs (hRPEs) were used to study AMD pathogenesis by using an established in vitro cellular model of the disease. Autophagy—a mechanism of intracellular degradation, which is altered during AMD, was studied in the hRPEs by using the autophagy inducer rapamycin and treated with the autophagy inhibitor bafilomycin A1. In addition, oxidative stress was induced by the hydrogen peroxide (H2O2) treatment of hRPEs. By using SR-FTIR microspectroscopy and multivariate analyses, the changes in the phosphate groups of nucleic acids, Amide I and II of the proteins, the carbonyl groups, and the lipid status in the hRPEs showed a significantly different pattern under oxidative stress/autophagy induction and inhibition. This biomolecular fingerprint can be evaluated in future drug discovery studies affecting autophagy and oxidative stress in AMD.
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Veder, Jean-Pierre, Kunal Patel, Graeme Clarke, Ewa Grygolowicz-Pawlak, Debbie S. Silvester, Roland De Marco, Ernö Pretsch et Eric Bakker. « Synchrotron Radiation/Fourier Transform-Infrared Microspectroscopy Study of Undesirable Water Inclusions in Solid-Contact Polymeric Ion-Selective Electrodes ». Analytical Chemistry 82, no 14 (15 juillet 2010) : 6203–7. http://dx.doi.org/10.1021/ac101009n.

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Al-Bakri, Amal G., Lina A. Dahabiyeh, Enam Khalil, Deema Jaber, Gihan Kamel, Nina Schleimer, Christian Kohler et Karsten Becker. « Synchrotron-Radiation-Based Fourier Transform Infrared Microspectroscopy as a Tool for the Differentiation between Staphylococcal Small Colony Variants ». Antibiotics 11, no 11 (11 novembre 2022) : 1607. http://dx.doi.org/10.3390/antibiotics11111607.

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Small colony variants (SCVs) are clinically significant and linked to persistent infections. In this study, synchrotron-radiation-based Fourier transform infrared (SR-FTIR) is used to investigate the microspectroscopic differences between the SCVs of Staphylococcus aureus (S. aureus) and diabetic foot Staphylococcus epidermidis (S. epidermidis) in two main IR spectral regions: (3050–2800 cm−1), corresponding to the distribution of lipids, and (1855–1500 cm−1), corresponding to the distribution of protein amide I and amide II and carbonyl vibrations. SR-FTIR successfully discriminated between the two staphylococcal species and between the SCV and the non-SCV strains within the two IR spectral regions. Combined S. aureus SCVs (SCVhMu) showed a higher protein content relative to the non-SCV wild type. Complemented S. aureus SCV showed distinguishable differences from the SCVhMu and the wild type, including a higher content of unsaturated fatty acids. An increase in the CH2/CH3 ratio was detected in S. epidermidis SCV samples compared to the standard control. Protein secondary structure in standard S. epidermidis and SCVs consisted mainly of an α-helix; however, a new shoulder at 1635 cm−1, assigned to β-sheets, was evident in the SCV. In conclusion, SR-FTIR is a powerful method that can discriminate between staphylococci species and to differentiate between SCVs and their corresponding natural strains.
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Ye, Danna, Philip Heraud, Rangsun Parnpai et Tong Li. « Reversal of Experimental Liver Damage after Transplantation of Stem-Derived Cells Detected by FTIR Spectroscopy ». Stem Cells International 2017 (2017) : 1–10. http://dx.doi.org/10.1155/2017/4585169.

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The transplantation of autologous BM-MSCs holds great potential for treating end-stage liver diseases. The aim of this study was to compare the efficiency of transplanted rBM-MSCs and rBM-MSC-derived differentiated stem cells (rBM-MSC-DSCs) for suppression of dimethylnitrosamine-injured liver damage in rat model. Synchrotron radiation Fourier-transform infrared (SR-FTIR) microspectroscopy was applied to investigate changes in the macromolecular composition. Transplantation of rBM-MSC-DSCs into liver-injured rats restored their serum albumin level and significantly suppressed transaminase activity as well as the morphological manifestations of liver disease. The regenerative effects of rBM-MSC-DSCs were corroborated unequivocally by the phenotypic difference analysis between liver tissues revealed by infrared spectroscopy. Spectroscopic changes in the spectral region from 1190–970 cm−1 (bands with absorbance maxima at 1150 cm−1, 1081 cm−1, and 1026 cm−1) indicated decreased levels of carbohydrates, in rBM-MSC-DSC-transplanted livers, compared with untreated and rBM-MSC--transplanted animals. Principal component analysis (PCA) of spectra acquired from liver tissue could readily discriminate rBM-MSC-DSC-transplanted animals from the untreated and rBM-MSC-transplanted animals. We conclude that the transplantation of rBM-MSC-DSCs effectively treats liver disease in rats and SR-FTIR microspectroscopy provides important insights into the fundamental biochemical alterations induced by the stem-derived cell transplantation, including an objective “signature” of the regenerative effects of stem cell therapy upon liver injury.
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Torrisi, Lorenzo, Valentina Venuti, Vincenza Crupi, Letteria Silipigni, Mariapompea Cutroneo, Giuseppe Paladini, Alfio Torrisi et al. « RBS, PIXE, Ion-Microbeam and SR-FTIR Analyses of Pottery Fragments from Azerbaijan ». Heritage 2, no 3 (10 juillet 2019) : 1852–73. http://dx.doi.org/10.3390/heritage2030113.

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The present work is aimed at the investigation of the ceramic bulk and pigmented glazed surfaces of ancient potteries dating back to XIX century A.D. and coming from the charming archeological site located in the Medieval Agsu town (Azerbaijan), a geographic area of special interest due to the ancient commercial routes between China, Asia Minor, and Europe. For the purpose of the study, complementary investigation tools have been exploited: non-destructive or micro-destructive investigation at elemental level by ion beam analysis (IBA) techniques, by using Rutherford Backscattering Spectrometry (RBS), Proton-Induced X-ray Emission (PIXE) spectroscopy and ion-microbeam analysis, and chemical characterization at microscopic level, by means of synchrotron radiation (SR) Fourier transform infrared (FTIR) microspectroscopy. The acquired information reveals useful for the identification of the provenance, the reconstruction of the firing technology, and finally, the identification of the pigment was used as a colorant of the glaze.
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Schmidt, Martin, Ulrich Schade et Michael Grunze. « Microspectroscopic observation of vibrational linear dichroism using polarization-modulated infrared synchrotron radiation ». Infrared Physics & ; Technology 49, no 1-2 (septembre 2006) : 69–73. http://dx.doi.org/10.1016/j.infrared.2006.01.005.

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Barón-Sola, Ángel, Margarita Toledo-Basantes, María Arana-Gandía, Flor Martínez, Cristina Ortega-Villasante, Tanja Dučić, Ibraheem Yousef et Luis E. Hernández. « Synchrotron Radiation-Fourier Transformed Infrared microspectroscopy (μSR-FTIR) reveals multiple metabolism alterations in microalgae induced by cadmium and mercury ». Journal of Hazardous Materials 419 (octobre 2021) : 126502. http://dx.doi.org/10.1016/j.jhazmat.2021.126502.

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Lin, Pin-Yen, Pei-Yu Huang, Yao-Chang Lee et Chen Siang Ng. « Analysis and comparison of protein secondary structures in the rachis of avian flight feathers ». PeerJ 10 (28 février 2022) : e12919. http://dx.doi.org/10.7717/peerj.12919.

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Avians have evolved many different modes of flying as well as various types of feathers for adapting to varied environments. However, the protein content and ratio of protein secondary structures (PSSs) in mature flight feathers are less understood. Further research is needed to understand the proportions of PSSs in feather shafts adapted to various flight modes in different avian species. Flight feathers were analyzed in chicken, mallard, sacred ibis, crested goshawk, collared scops owl, budgie, and zebra finch to investigate the PSSs that have evolved in the feather cortex and medulla by using nondestructive attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR). In addition, synchrotron radiation-based, Fourier transform infrared microspectroscopy (SR-FTIRM) was utilized to measure and analyze cross-sections of the feather shafts of seven bird species at a high lateral resolution to resolve the composition of proteins distributed within the sampled area of interest. In this study, significant amounts of α-keratin and collagen components were observed in flight feather shafts, suggesting that these proteins play significant roles in the mechanical strength of flight feathers. This investigation increases our understanding of adaptations to flight by elucidating the structural and mechanistic basis of the feather composition.
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Kumar, Selvaraj Rajesh, Ya-Hui Hsu, Truong Thi Tuong Vi, Jong-Hwei Su Pang, Yao-Chang Lee, Chia-Hsun Hsieh et Shingjiang Jessie Lue. « Graphene Oxide-Induced Protein Conformational Change in Nasopharyngeal Carcinoma Cells : A Joint Research on Cytotoxicity and Photon Therapy ». Materials 14, no 6 (13 mars 2021) : 1396. http://dx.doi.org/10.3390/ma14061396.

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The objectives of this work aim to investigate the interaction and cytotoxicity between nanometric graphene oxide (GO) and nasopharyngeal carcinoma cells (NPC-BM1), and possible application in photon therapy. GO nanosheets were obtained in the size range of 100–200 nm, with a negative surface charge. This nanometric GO exhibited a limited (<10%) cytotoxicity effect and no significant dimensional change on NPC-BM1 cells in the tested GO concentration range (0.1–10 µg·mL−1). However, the secondary protein structure was modified in the GO-treated NPC-BM1 cells, as determined through synchrotron radiation-based Fourier transform infrared microspectroscopy (SR-FTIRM) mapping. To further study the cellular response of GO-treated NPC-BM1 cancer cells at low GO concentration (0.1 µg·mL−1), photon radiation was applied with increasing doses, ranging from 2 to 8 Gy. The low radiation energy (<5 Gy) did not cause significant cell mortality (5–7%). Increasing the radiation energy to 6–8 Gy accelerated cell apoptosis rate, especially in the GO-treated NPC-BM1 cells (27%). This necrosis may be due to GO-induced conformational changes in protein and DNA/RNA, resulting in cell vulnerability under photon radiation. The findings of the present work demonstrate the potential biological applicability of nanometric GO in different areas, such as targeted drug delivery, cellular imaging, and radiotherapy, etc.
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Vargas-Caraveo, Alejandra, Hiram Castillo-Michel, Gloria Erika Mejia-Carmona, David Guillermo Pérez-Ishiwara, Marine Cotte et Alejandro Martínez-Martínez. « Preliminary studies of the effects of psychological stress on circulating lymphocytes analyzed by synchrotron radiation based-Fourier transform infrared microspectroscopy ». Spectrochimica Acta Part A : Molecular and Biomolecular Spectroscopy 128 (juillet 2014) : 141–46. http://dx.doi.org/10.1016/j.saa.2014.02.148.

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