Bibliographies thématiques / Synchrotron Radiation Infrared Microspectroscopy

Littérature scientifique sur le sujet « Synchrotron Radiation Infrared Microspectroscopy »

Auteur : Grafiati

Publié le 11 mars 2023

Créez une référence correcte selon les styles APA, MLA, Chicago, Harvard et plusieurs autres

Choisissez une source :

Consultez les listes thématiques d’articles de revues, de livres, de thèses, de rapports de conférences et d’autres sources académiques sur le sujet « Synchrotron Radiation Infrared Microspectroscopy ».

À côté de chaque source dans la liste de références il y a un bouton « Ajouter à la bibliographie ». Cliquez sur ce bouton, et nous générerons automatiquement la référence bibliographique pour la source choisie selon votre style de citation préféré : APA, MLA, Harvard, Vancouver, Chicago, etc.

Vous pouvez aussi télécharger le texte intégral de la publication scolaire au format pdf et consulter son résumé en ligne lorsque ces informations sont inclues dans les métadonnées.

Articles de revues sur le sujet "Synchrotron Radiation Infrared Microspectroscopy"

Michaelian, Kirk H., Mark D. Frogley, Gianfelice Cinque et Luca Quaroni. « Infrared spectra of micro-structured samples with microPhotoacoustic spectroscopy and synchrotron radiation ». Analyst 145, n^o 4 (2020) : 1483–90. http://dx.doi.org/10.1039/c9an01281h.

Texte intégral

Styles APA, Harvard, Vancouver, ISO, etc.

Qian, Jiang, Xue Gao, Ya-Di Wang, Xue-Ling Li, Jun Hu et Jun-Hong Lü. « Synchrotron Infrared Microspectroscopy for Stem Cell Research ». International Journal of Molecular Sciences 23, n^o 17 (30 août 2022) : 9878. http://dx.doi.org/10.3390/ijms23179878.

Texte intégral

Résumé :

Stem cells have shown great potential functions for tissue regeneration and repair because of their unlimited self-renewal and differentiation. Stem cells reside in their niches, making them a hotspot for the development and diagnosis of diseases. Complex interactions between niches and stem cells create the balance between differentiation, self-renewal, maturation, and proliferation. However, the multi-facet applications of stem cells have been challenged since the complicated responses of stem cells to biological processes were explored along with the limitations of current systems or methods. Emerging evidence highlights that synchrotron infrared microspectroscopy, known as synchrotron radiation-based Fourier transform infrared microspectroscopy, has been investigated as a potentially attractive technology with its non-invasive and non-biological probes in stem cell research. With their unique vibration bands, the quantitative mapping of the content and distribution of biomolecules can be detected and characterized in cells or tissues. In this review, we focus on the potential applications of synchrotron infrared microspectroscopy for investigating the differentiation and fate determination of stem cells.

Styles APA, Harvard, Vancouver, ISO, etc.

Martínez-Rovira, Immaculada, Olivier Seksek, Josep Puxeu, Joan Gómez, Martin Kreuzer, Tanja Dučić, Maria Josep Ferreres, Manel Artigues et Ibraheem Yousef. « Synchrotron-based infrared microspectroscopy study on the radiosensitization effects of Gd nanoparticles at megavoltage radiation energies ». Analyst 144, n^o 18 (2019) : 5511–20. http://dx.doi.org/10.1039/c9an00792j.

Texte intégral

Styles APA, Harvard, Vancouver, ISO, etc.

Zhu Huachun, 朱化春, 佟亚军 Tong Yajun, 吉特 Ji Te, 彭蔚蔚 Peng Weiwei, 张增艳 Zhang Zengyan, 陈敏 Chen Min et 肖体乔 Xiao Tiqiao. « Spatial Resolution Measurement of Synchrotron Radiation Infrared Microspectroscopy Beamline ». Acta Optica Sinica 35, n^o 4 (2015) : 0430002. http://dx.doi.org/10.3788/aos201535.0430002.

Texte intégral

Styles APA, Harvard, Vancouver, ISO, etc.

Carr, G. L. « Resolution limits for infrared microspectroscopy explored with synchrotron radiation ». Review of Scientific Instruments 72, n^o 3 (2001) : 1613. http://dx.doi.org/10.1063/1.1347965.

Texte intégral

Styles APA, Harvard, Vancouver, ISO, etc.

Rosendahl, Scott M., Ferenc Borondics, Tim E. May, Tor M. Pedersen et Ian J. Burgess. « Interface for time-resolved electrochemical infrared microspectroscopy using synchrotron infrared radiation ». Review of Scientific Instruments 82, n^o 8 (août 2011) : 083105. http://dx.doi.org/10.1063/1.3624693.

Texte intégral

Styles APA, Harvard, Vancouver, ISO, etc.

Ikemoto, Yuka, Manako Tanaka, Tomohiro Higuchi, Toshirou Semba, Taro Moriwaki, Emi Kawasaki et Masayoshi Okuyama. « Infrared Synchrotron Radiation and Its Application to the Analysis of Cultural Heritage ». Condensed Matter 5, n^o 2 (9 avril 2020) : 28. http://dx.doi.org/10.3390/condmat5020028.

Texte intégral

Résumé :

Infrared synchrotron radiation (IR-SR) is a broad-band light source. Its brilliance is the main advantage for microspectroscopy experiments, when the limited size of the sample often prevents the use of conventional thermal radiation sources. Cultural heritage materials are delicate and valuable; therefore, nondestructive experiments are usually preferred. Nevertheless, sometimes, small pieces can be acquired in the process of preservation and conservation. These samples are analyzed by various experimental techniques and give information about the original material and current condition. In this paper, four attempts to analyze cultural heritage materials are introduced. All these experiments are performed at the microspectroscopy station of IR beamline BL43IR in SPring-8.

Styles APA, Harvard, Vancouver, ISO, etc.

Wetzel, David L., John A. Reffner et Gwyn P. Williams. « Synchrotron infrared microspectroscopy challenges difficult analytical problems ». Proceedings, annual meeting, Electron Microscopy Society of America 54 (11 août 1996) : 262–63. http://dx.doi.org/10.1017/s0424820100163770.

Texte intégral

Résumé :

Micro spectroscopy of molecular species in situ takes advantage of biological concentration by nature of certain molecules within the bulk specimen. Localized hetergeniety is readily documented. Order or randomness may be established for substances that are present in a quantities below the detection limit in the bulk but that may be present in a measurable amount when acute probing is done in the correct region of the specimen. Spectral resolution with a microbeam directed at a relatively small spot from which spectroscopic data is obtained makes this task readily possible. Spectral resolution is achieved with optical efficiency of modern infrared micro spectrometers in which signal is conserved and noise minimized. Improvement of S/N is routinely done by coadding multiple scans however reducing the aperture by half reduces the signal by at least four and doubling S/N requires taking data for four times as long. Thus even if a diffraction limiting situation does not arise one of these desirable goals of 1) good spectral separation (small aperture size), 2) high S/N (good low-noise spectra) and 3) short time of data accumulation must be compromised. This is true with even the most optically efficient instrument however substituting infrared radiation from the vacuum UV storage ring of the National Synchrotron Light Source (Brookhaven National Laboratory) for the conventional thermal (glowbar) instrument source makes it possible to achieve all three goals without the necessity of a severe compromise on at least one of them. The synchrotron beam is 2-3 orders of magnitude brighter, has no thermal noise, and has very low divergence. Aperturing the small concentrated beam results in a relatively small percentage loss in beam intensity.

Styles APA, Harvard, Vancouver, ISO, etc.

Lerch, Ph, P. Dumas, T. Schilcher, A. Nadji, A. Luedeke, N. Hubert, L. Cassinari et al. « Assessing noise sources at synchrotron infrared ports ». Journal of Synchrotron Radiation 19, n^o 1 (25 novembre 2011) : 1–9. http://dx.doi.org/10.1107/s0909049511041884.

Texte intégral

Résumé :

Today, the vast majority of electron storage rings delivering synchrotron radiation for general user operation offer a dedicated infrared port. There is growing interest expressed by various scientific communities to exploit the mid-IR emission in microspectroscopy, as well as the far infrared (also called THz) range for spectroscopy. Compared with a thermal (laboratory-based source), IR synchrotron radiation sources offer enhanced brilliance of about two to three orders of magnitude in the mid-IR energy range, and enhanced flux and brilliance in the far-IR energy range. Synchrotron radiation also has a unique combination of a broad wavelength band together with a well defined time structure. Thermal sources (globar, mercury filament) have excellent stability. Because the sampling rate of a typical IR Fourier-transform spectroscopy experiment is in the kHz range (depending on the bandwidth of the detector), instabilities of various origins present in synchrotron radiation sources play a crucial role. Noise recordings at two different IR ports located at the Swiss Light Source and SOLEIL (France), under conditions relevant to real experiments, are discussed. The lowest electron beam fluctuations detectable in IR spectra have been quantified and are shown to be much smaller than what is routinely recorded by beam-position monitors.

Styles APA, Harvard, Vancouver, ISO, etc.

Lin Yuecheng, 林乐诚, 佟亚军 Tong Yajun, 吉特 Ji Te, 彭蔚蔚 Peng Weiwei, 肖体乔 Xiao Tiqiao, 朱化春 Zhu Huachun et 陈敏 Chen Min. « Synchrotron Radiation Infrared Three-Dimensional Microspectroscopy Based on Point Scanning Method ». Acta Optica Sinica 40, n^o 3 (2020) : 0334001. http://dx.doi.org/10.3788/aos202040.0334001.

Texte intégral

Styles APA, Harvard, Vancouver, ISO, etc.

Plus de sources

Thèses sur le sujet "Synchrotron Radiation Infrared Microspectroscopy"

Birarda, Giovanni. « Development of microfluidic devices for biomedical applications of synchrotron radiation infrared microspectroscopy ». Doctoral thesis, Università degli studi di Trieste, 2011. http://hdl.handle.net/10077/4475.

Texte intégral

Résumé :

2009/2010
ABSTRACT DEVELOPMENT OF MICROFLUIDIC DEVICES FOR BIOMEDICAL APPLICATIONS OF SYNCHROTRON RADIATION INFRARED MICROSPECTROSCOPY by Birarda Giovanni The detection and measurement of biological processes in a complex living system is a discipline at the edge of Physics, Biology, and Engineering, with major scientific challenges, new technological applications and a great potential impact on dissection of phenomena occurring at tissue, cell, and sub cellular level. The present PhD Thesis dealt with the development of methodologies and technologies to transform InfraRed MicroSpectroscopy (IRMS) into a mature technique to observe in real time biological events, and improving its ability to perform in vitro bio-experiments under physiological conditions. This goal has been achieved through the exploitation of microfabrication techniques to realize lab-on-chip (LOCs) transparent both in the Infrared and Visible region (IR-Vis), which allows measuring living cells. Up to now, IRMS has been almost exclusively employed for studying fixed cellular samples or tissues, allowing acquiring only “still frames” of the phenomena under investigation. The reason for that is to be ascribed both to the spectroscopic difficulties in working in water based environment and to the manufacturing constrains of the most common IR transparent materials, that limit the design flexibility of LOC devices suitable for IR analysis. We have overcome the so called “water absorption barrier” by extending microfluidic concepts to calcium fluoride, implementing innovative fabrication solutions for the realization of custom devices for IRMS studies of living cells subjected to different chemical and physical stimuli. Exploiting the high brightness of Synchrotron Radiation (SR) IR sources, that allows sampling at diffraction limited spatial resolution, we demonstrated the feasibility of the detection of intra-cellular processes. In parallel, novel strategies for IR data acquisition and analysis have been developed, opening the possibility to execute novel original experiments. Our studies were focused on the immune system, and in particular in evaluating the biochemical rearrangements characterizing human circulating leukocytes during their deformation, either when induced by purely mechanical stimuli or in response to a chemical gradient. Thanks to the microfabrication approach, we were able to mimic the cellular microenvironment both for studying pressure-driven micro-capillary circulation and chemically-driven extravasations of white blood cells. The present Thesis demonstrates that the “synergy of micro-approaches”, or rather the combination of micro-fabrication and IR micro-spectroscopy, can be exploited for extending the frontiers of Fourier Transform Infrared Spectroscopy (FTIR) to unexplored fields of life sciences. Through the careful control of the cellular microenvironment, crucial for an accurate data analysis as well as fundamental for the reliability of biological conclusions, some light could be shed on phenomena never investigated with IRMS, such as mechano-biology we directly explored, pulling down the water-barrier.
RIASSUNTO SVILUPPO DI DISPOSITIVI MICROFLUIDICI PER APPLICAZIONI BIOMEDICHE DELLA MICROSPETTROSCOPIA INFRAROSSA CON RADIAZIONE DI SINCROTRONE di Birarda Giovanni L’identificazione e la quantificazione di processi biologici in un complesso sistema vivente può essere ritenuta una disciplina al confine tra la Fisica, la Biologia e l’Ingegneria, con importanti sfide scientifiche, innovazioni tecnologiche e un grande impatto sulla dissezione di fenomeni a livello tissutale, cellulare e sub cellulare. Il presente lavoro di Dottorato ha avuto come obiettivo lo sviluppo di metodologie e tecnologie atte a rendere la MicroSpettroscopia InfraRossa (MSIR) una tecnica matura allo studio in tempo reale di fenomeni biologici, permettendo di effettuare esperimenti “in vitro” in condizioni fisiologiche. Questo obiettivo è stato raggiunto tramite l’utilizzo delle tecniche di microfabbricazione per la realizzazione di un “Lab-on-Chip” (LOC) trasparente sia nella regione dell’infrarosso che nel visibile, tramite il quale misurare cellule vive. Infatti fin’ora la MISR è stata impiegata quasi esclusivamente per lo studio di campioni di tessuti o di cellule fissati, permettendo di registrare solo “singoli fotogrammi” dei fenomeni sotto indagine. La ragione di questa limitazione è da imputarsi alle difficoltà spettroscopiche che si incotrano nell’investigazione di sistemi acquosi e ai limiti di fabbricazione dei più comuni materiali IR trasparenti, che hanno limitato la flessibilità di design necessaria alla realizzazione di LOC adatti alle analisi tramite MSIR. Siamo riusciti a superare la cosiddetta “barriera di assorbimento dell’acqua” tramite l’estensione dei concetti della microfluidica e dellamicrofabbricazione al calcio fluoruro, implementando soluzioni fabbricative che hanno permesso lo studio tramite MSIR di cellule viventi sottoposte a differenti stimoli sia di natura chimica che fisica. Grazie all’alta brillanza della Radiazione di Sinctrotrone (SR) IR, che permette il campionamento con una risoluzione spaziale al limte di diffrazione, abbiamo dimostrato la fattibilità dell’individuazione dei processi intra celluari. Contemporaneamente sono state sviluppate nuove strategie per l’acquisizione dei dati e per la loro analisi, permettendo il design di esperimenti innovativi. I nostri studi si sono concentrati sullo studio del sistema immunitario, in particolare nella valutazione della risposta biochimica caraterristica dei leucociti circolanti durante la loro deformazione, sia indotta da cause di tipo puramente meccanico, sia in risposta a gradienti chimici. Grazie alla microfabbricazione, siamo stati capaci di simulare il microambiente cellulare sia per lo studio dei globuli bianchi durante la circolazione microcapillare sia durante l’extravasazione indotta da gradienti chimici. La presente Tesi dimostra che la sinergia dei “micro” approcci, o piuttosto la combinazione di microfabbricazione e microspettroscopia IR, può essere usata per estendere le frontiere della MSIR a nuovi campi nello studio delle scienze della vita. Attraverso il preciso controllo del microambiente cellulare, cruciale per un’accurata analisi dei dati e fondamentale per l’attendibilità delle conclusioni biologiche, si possono chiarire fenomeni finora mai investigati tramite MSIR, come la meccano-biologia che abbiamo esplorato direttamente, abbattendo la barriera dell’acqua.
XXIII Ciclo
1981

Styles APA, Harvard, Vancouver, ISO, etc.

Dokken, Kenneth M. « Infrared microspectroscopy of plants : use of synchrotron radiation infrared microspectroscopy to study plant root anatomy and to monitor the fate of organic contaminants in those roots ». Diss., Manhattan, Kan. : Kansas State University, 2006. http://hdl.handle.net/2097/164.

Texte intégral

Styles APA, Harvard, Vancouver, ISO, etc.

Pearson, Martin. « Synchrotron infrared microspectroscopy ». Thesis, University of Nottingham, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.324753.

Texte intégral

Styles APA, Harvard, Vancouver, ISO, etc.

Peng, Chengyuan. « Diagnosis of Steatosis, Precancerous Lesions and Hepatocellular Carcinoma Using Infrared Microspectroscopy ». Thesis, Paris 11, 2015. http://www.theses.fr/2015PA11T032.

Texte intégral

Résumé :

Carcinome hépatocellulaire (CHC) est le sixième cancer et la deuxième cause de mortalité par cancer dans le monde. Dans la majorité des cas, le CHC se développe sur une maladie chronique associée à des étiologies variées telles que l'infection par le virus de l'hépatite B ou l’hépatite C, la consommation excessive d'alcool et des maladies métaboliques. Le développement des maladies chroniques du foie qui conduisent à la cirrhose puis au cancer induisent des modifications de la composition chimique des cellules et des tissus. En effet, la carcinogenèse hépatique est un processus en plusieurs étapes caractérisé par la progression de nodules de régénération, de nodules dysplasiques de bas grade puis de grade et enfin du CHC. Le traitement du CHC reste difficile et la transplantation du foie est la seule option thérapeutique curative à long terme. Le problème est qu'il n'y a pas de marqueur objectifs et quantifiables pour contrôler la qualité d’un greffon. Des biomarqueurs spécifiques marquant la progression du CHC font également défauts.Dans ce travail de thèse, nous avons évalué l’intérêt de la microspectroscopie infrarouge (IR) pour le diagnostic de la stéatose, qui est le facteur le plus important affectant la reprise de la fonction hépatique après greffe de foie. La microspectroscopie infrarouge permet de détecter de façon qualitative et quantitative les caractéristiques biochimiques liées aux différents constituants moléculaires présents dans l'échantillon biologique. Nos travaux ont montré que la progression de la stéatose hépatique correspond non seulement à l'accumulation de lipides, mais également à des changements spectaculaires dans la composition qualitative du tissu. En effet, le bas grade de stéatose présente une diminution de la teneur en glycogène et une augmentation concomitante de lipides par rapport au foie normal. La stéatose intermédiaire montre une augmentation de glycogène et des changements majeurs sont observés en ce qui concerne les lipides, avec une contribution significative des acides gras estérifiés, des chaînes de carbone allongées et des lipides insaturés. Ces caractéristiques sont encore plus prononcées dans les hauts degrés de stéatose. De plus, nous avons mis en évidence que des changements biochimiques majeurs se produisent dans la partie non-stéatosique du tissu malgré son aspect normal sur le plan histologique, ce qui suggère que l’organe dans son ensemble reflète le degré de la stéatose.La deuxième partie de la thèse est focalisée la carcinogenèse hépatique. Il s’agit d’un processus en plusieurs étapes qui se caractérise dans la plupart des foies cirrhotiques par la progression de nodules hyperplasiques de régénération vers des lésions précancéreuses telles que les nodules dysplasiques de bas grade puis de haut grade et enfin le CHC. Le diagnostic différentiel entre nodules dysplasiques en particulier de haut garde et CHC reste extrêmement difficile. Nous avons abordé le potentiel de la microspectroscopie IR pour le diagnostic des nodules cirrhotiques. Nous avons observé de profondes modifications de la composition biochimique du foie pathologique. En effet, des changements importants ont été détectés dans la composition des lipides, des protéines et des sucres mettant en évidence la reprogrammation métabolique dans la carcinogenèse. Les principaux changements ont été observés dans le domaine de fréquence 950-1480 cm-1 dans lequel plusieurs bandes permettaient la discrimination des nodules cirrhotiques, dysplasiques et tumoraux. Enfin, nous avons montré que le diagnostic peut être réalisé à l’aide d’un microscope de laboratoire qui peut être facilement mis en œuvre en milieu hospitalier
Hepatocellular carcinoma (HCC) is the sixth most common neoplasm and the second most common cause of death in the world. Hepatocarcinogenesis is a multistep process characterized in patients with chronic liver diseases by a spectrum of hepatic nodules that mark the progression from regenerative nodules to dysplastic lesions followed by HCC. Liver transplantation remains the curative therapeutic option able to treat both the HCC and the underlying liver disease. The issue is that there is no objective and quantifiable marker for quality control of liver graft. Specific biomarkers of early stages of HCC are also an unmet need.In this study, we have evaluated the potential of infrared (IR) microspectroscopy for the diagnosis of steatosis, one of the most important factors affecting the liver allograft function. Vibrational microspectroscopy, such as Fourier transform infrared microspectroscopy (FTIR), allows detecting spectral characteristics associated with different molecular components present in the biological sample, both qualitatively and quantitatively. Our first working hypothesis was that the progression of liver steatosis corresponds not only to the accumulation of lipids but also to dramatic changes in the qualitative composition of tissue. Indeed, a lower grade of steatosis showed a decrease in glycogen content and concomitant increase in lipids in comparison with normal liver. Intermediate steatosis exhibited an increase in glycogen and major changes in lipids, with a significant contribution of esterified fatty acids with elongated carbon chains and unsaturated lipids, and these features were more pronounced in a high grade of steatosis. Furthermore, we have shown, that FTIR approach allows a systemic discrimination of morphological features, leading to a separate investigation of steatotic vesicles and the non-steatotic counterpart of the tissue. This highlighted the fact that dramatic biochemical changes occur in the non-steatotic part of the tissue also despite its normal histological aspect, suggesting that the whole tissue reflects the grade of steatosis. The second part of the thesis focused on hepatocarcinogenesis; a multistep process that is characterized in most cirrhotic livers by the progression from hyperplastic regenerative nodules to low grade dysplastic nodules (LGDN), high grade dysplastic nodules (HGDN) and finally small HCC which corresponds either to vaguely nodular well differentiated HCC so called early HCC or to distinctly nodular moderately differentiated hepatocellular carcinomas. Since the differential diagnosis between precancerous dysplastic nodules and early HCC remains extremely difficult, we addressed the potential of FTIR microspectroscopy for grading cirrhotic nodules. The study was focused on 39 surgical specimens including normal livers as controls, dysplastic nodules, early HCC and the progressed HCC. Profound alterations of the biochemical composition of the pathological liver were demonstrated by FTIR microspectroscopy. Indeed, dramatic changes were observed in lipids, proteins and sugars highlighting the metabolic reprogramming in carcinogenesis. The major changes were observed in the frequency domain 950-1480 cm-1 in which several bands allowed significant discrimination of cirrhotic nodules, dysplastic lesions and HCC. Finally, a significant discrimination between benign, dysplastic nodules and early HCC remained possible using a FTIR microscope equipped with a laboratory-based infrared source that can be easily implemented in hospital environment. In conclusion, our study positions FTIR microspectroscopy as a versatile and powerful approach for investigating liver diseases, such as steatosis, dysplastic lesions and cancer. Further studies on larger series of patients as well as on biopsies will allow confirming the clinical reliability of such spectral signatures. Therefore, we anticipate that FTIR microspectroscopy will open new avenue in clinical diagnosis

Styles APA, Harvard, Vancouver, ISO, etc.

Srichan, Sirinart. « Synchrotron Based Fourier Transform Infrared Microspectroscopy and Fluorescence Microscopy : Application on Photodynamic Treated Cancer Cells ». Paris 7, 2009. http://www.theses.fr/2009PA077181.

Texte intégral

Résumé :

Lors de ce travail nous avons étudié l'effet photodynamique (PDT) de l'Hypocrelline A (HA) sur quatre lignées de cellules cancéreuses (HeLa, Calu-1, K562, K562 RSTI571) par une approche biophysique. Nous avons combiné les informations obtenues par microscopie de fluorescence confocale, tests de cytotoxicité et microspectroscopie infrarouge à transformée de Fourier (IRTF) pour éclairer la physico-chimie de la PDT. La très haute sensibilité de la microscopie de fluorescence confocale permet de détecter la fluorescence intrinsèque de HA à des concentrations de l'ordre du nanomolaire, de suivre sa pénétration dans les cellules et de réaliser des cartes 3D haute résolution montrant sa distribution. La microspectroscopie IRTF est utilisée à fin de détecter des changements de la composition chimique globale de la cellule et les modifications de la structure des principaux composants cellulaires lors du traitement. L'utilisation d'une source synchrotron autorise une résolution subcellulaire qui devrait permettre de discriminer les réactions à l'intérieur des différents compartiments cellulaires tel que le noyau et le cytoplasme. Les tests de cytoxicité et l'utilisation de marqueurs fluorescents ont permis de comparer les effets des traitements croissants sur les différentes lignées cellulaires et d'optimiser les conditions de traitement pour obtenir différents types de mort cellulaire. Nous avons également montré que la combinaison de la microspectroscopie IRTF et de la microscopie de fluorescence sur un même instrument était possible et permettait de mesurer simultanément le spectre IRTF d'une cellule unique et le type de mort cellulaire qu'elle a subi. Les différentes approches utilisées dans ce travail se combinent pour montrer que HA ne pénètre pas dans le noyau mais est localisée dans le cytoplasme et accumulée autour du noyau après 24 heures d'incubation ; que les 4 lignées cellulaires réagissent de façon similaire ; que des modifications de la composition chimique des noyaux des cellules sont détectables par microspectroscopie IRTF mais que les cytoplasmes des cellules adhérentes sont trop minces pour y détecter des altérations sauf dans la région périnucléaire. Même en absence de pénétration de HA dans le noyau, la microspectroscopie IRTF permet de détecter des changements de la structure secondaire des protéines du noyau et des perturbations des membranes du réticulum endoplasmique et de l'appareil de Golgi sous l'action de espèces oxygénées réactives générées par la PDT
In this thesis, we studied the photodynamic effects of Hypocrellin A on four cancer cell lines using fluorescence microscopy, cytotoxicity tests and FT-IR microspectroscopy. As HA is a natural fluorescent substance, we used this intrinsic property to observe its localization in HeLa cells by fluorescence microscopy. The fluorescence images revealed that HA did not penetrated into the nucleus but localized in the cytoplasm and aggregated perinuclearly after 24 hours of incubation. MTT viability assay was performed to evaluate the optimum PDT conditions (HA and light dose) which induced cell death in the four cell lines HeLa, Calu-1, K562, K562 RSTI571. The dark toxicity of HA on ail cell lines is low, but is increasing dose-dependently. The phototoxicity of HA on all cell lines was increasing in both HA dose and light dose dependent manner. The light irradiation alone affected only negligibly the cell survival. HeLa cells are sensitive to HA PDT than Calu-1 cells. We found also that K562 and K562 RSTI are sensitive to HA. Moreover the synergetic effect of HA and Glivec® on K562 RSTI was observed. FT-IR microspectroscopy detected the changes in the secondary structure of proteins exhibiting an increase of beta sheets characteristics frequency affected by ROS generated from PDT. , with a predominant shoulder at around 1630 cm"1 (for the Amide I band) and 1530 cm"1 (for the Amide II band). Moreover, a slight decrease of the lipid intensity was noticed. Coupling fluorescence microscopy and FT-IR microscopy was carried out on the same instrument. Fluorescence microscopy could reveal the modes of cell death while FT-IR microspectroscopy showed effect of HA PDT on the secondary structure of proteins. All approaches carried out in this thesis revealed that even HA did not penetrate in the nucleus but there are changes in secondary structure of proteins in nucleus which can be observed by FT-IR spectromicroscopy

Styles APA, Harvard, Vancouver, ISO, etc.

Bonwell, Emily Susanne. « Determination of endosperm protein secondary structure in hard wheat breeding lines using synchrotron infrared microspectroscopy and revelation of secondary structural changes in protein films with thermal processing ». Thesis, Manhattan, Kan. : Kansas State University, 2008. http://hdl.handle.net/2097/593.

Texte intégral

Styles APA, Harvard, Vancouver, ISO, etc.

Koc, Hicran. « Infrared chemical imaging of germinated wheat : early nondestructive detection and microspectroscopic imaging of kernel thin cross sections in Situ ». Thesis, Manhattan, Kan. : Kansas State University, 2007. http://hdl.handle.net/2097/512.

Texte intégral

Styles APA, Harvard, Vancouver, ISO, etc.

Garip, Sebnem. « Investigation Of Drug-related Changes On Bone Tissues Of Rat Animal Models In Healthy And Disease States ». Phd thesis, METU, 2012. http://etd.lib.metu.edu.tr/upload/12614998/index.pdf.

Texte intégral

Résumé :

Disease- and drug-related bone disorders are rapidly increasing in the population. The drugs which are used for the treatment of neurodegenerative diseases and metabolic derangements, may have negative or positive effects on bone tissues. In the first study, the possible side-effects of Carbamazepine and epileptic seizures on bone structure and composition were investigated by FTIR and synchrotron-FTIR microspectroscopy, AFM and micro- and nano-hardness analysis. The effects on the blood parameters, bone turnover and vitamin D metabolism were also investigated by ELISA and western blot analysis. The current study provides the first report on differentiation of the effects of both epileptic seizures and AED therapy on bones. Besides Carbamazepine treatment, seizures also caused a decrease in the strength of bone. The biochemical data showed that both the epileptic and drug-treated groups decreased vitamin D levels by increasing the vitamin D catabolism enzyme
25-hydroxyvitamin D-24-hydroxylase. In the second study, the possible pleiotropic (positive) effects of cholesterol lowering drug
Simvastatin on bones were investigated by ATR-FTIR spectroscopy. The current study provides the first report on dose-dependent effects of simvastatin on protein structure and lipid conformation of bones. ATR-FTIR studies showed that although both high and low dose simvastatin strengthen bones, low dose simvastatin treatment is much more effective in increasing bone strength. Neural network analysis revealed an increased antiparallel and aggregated beta sheet and random coil in the protein secondary structure of high dose group implying a protein denaturation. Moreover, high dose may induce lipid peroxidation which limit the pleiotropic effects of high dose treatment on bones. This study clearly demonstrated that using low dose simvastatin is safer and more effective for bone health than high dose simvastatin treatment.

Styles APA, Harvard, Vancouver, ISO, etc.

Partouche, David. « Analyse de l’assemblage de peptides amyloïdes bactériens ». Thesis, Université Paris-Saclay (ComUE), 2018. http://www.theses.fr/2018SACLX084/document.

Texte intégral

Résumé :

Hfq est une protéine bactérienne qui a un rôle pleiotropique. La principale fonction de la protéine Hfq bactérienne consiste à répondre aux stress que peut rencontrer la bactérie lors d’un changement environnemental, en utilisant essentiellement un contrôle post-transcriptionnel. La protéine, par sa capacité à interagir avec les ARN et notamment les petits ARN non codant, permet ainsi une régulation rapide de l’expression génétique. En outre la protéine interagit aussi avec l’ADN qu’elle aide à se structurer. Les mutations dans le gène qui code pour Hfq ont des effets pleïotropes (déterminant plusieurs caractères phénotypiques).D’un point de vue structural, la protéine adopte un repliement de type Sm, caractérisé par un oligomère toroïdal reposant sur la formation d’un feuillet β continu à 30 brins. Cependant, outre cette région Sm N-terminale, Hfq possède également une région C-terminale (CTR) de taille et de séquence variables selon les bactéries. Mon travail de thèse a porté sur l’analyse de cette région CTR chez la bactérie Escherichia coli. Cette région a en effet la capacité de former une structure de type amyloïde : structures auto-assemblées in vivo, à proximité de la membrane interne et dans le nucléoïde.Par l’utilisation de diverses techniques physico-chimiques (microscopie moléculaire, spectroscopie et microscopie infrarouge, dichroïsme circulaire et diffusion aux petits angles), mon travail a consisté à caractériser l’assemblage de cette région de Hfq ainsi que les facteurs l’influençant en particulier la présence d’acide nucléique. Une partie de mon travail de thèse a aussi consisté à mettre en place une méthode d’imagerie corrélative innovante permettant d’analyser la signature chimique et morphologique d’une fibre amyloïde unique. Mon travail a enfin porté sur l’analyse de l’effet de composés inhibant l’agrégation de la structure amyloïde, ce qui pourrait constituer une piste pour développer une nouvelle classe d’antibiotiques
Hfq is a pleiotropic bacterial protein that determines several phenotypic characteristics. Its main function is to facilitate responses to stresses that bacteria may encounter during environmental changes, mainly by using post-transcriptional genetic control. The protein, by its capacity to interact with RNA, in particular small non-coding RNA, enables a rapid regulation of gene expression. In addition, the protein also interacts with DNA and compacts it. From a structural point of view, the protein adopts an Sm-like fold, characterized by a toroidal oligomer formed by a continuous 30-stranded β-sheet. Besides its conserved N-terminal Sm domain, Hfq also possesses a C-terminal region (CTR) that can vary in size and sequence between bacteria. My PhD work focused on the analysis of this CTR region in Escherichia coli bacteria. Indeed, this region has the capacity to form an amyloid structure. This structural dynamic is related to the formation of self-assembled structures in vivo, in the proximity of the inner membrane and in the nucleoid.Using various physicochemical techniques (molecular microscopy, spectroscopy and infrared microscopy, circular dichroism and small angle X-ray scattering), my work consisted in characterizing the assembly of this region of Hfq, as well as the factors influencing its assembly (in particular, the presence of nucleic acids). A part of my work consisted in setting up an innovative correlative–imaging method to analyze the chemical and morphological signature of a single amyloid fibre. Finally, my work focused on the analysis of the effect of compounds that inhibit the aggregation of the amyloid structure, which could constitute a new way to develop a novel class of antibiotics

Styles APA, Harvard, Vancouver, ISO, etc.

Anantharajah, Anusanth. « Spectroscopie infrarouge lointain et moyen à haute résolution par transformée de Fourier de molécules complexes d’intérêt atmosphérique : ClNO₂, Cl₂CO et ClONO₂ ». Electronic Thesis or Diss., Université Paris Cité, 2020. https://theses.md.univ-paris-diderot.fr/ANANTHARAJAH_Anusanth_va2.pdf.

Texte intégral

Résumé :

La mesure des concentrations d’espèces à l’état de trace, susceptibles d’avoir un impact notable sur la santé, le climat ou la stabilité de la couche d’ozone constitue un véritable défi. Les prochaines missions spatiales, prévues à haute sensibilité, apporteront un progrès si et seulement si les paramètres spectraux nécessaires sont disponibles. Pour certaines espèces d’intérêt atmosphérique telles que le chlorure de nitryle (ClNO₂), le phosgène (Cl₂CO) et le nitrate de chlore (ClONO₂), les données spectroscopiques sont incomplètes ou quasiment inexistantes. Le défi dans cette thèse consiste à obtenir des paramètres spectroscopiques (positions et intensités de raies ou sections efficaces d’absorption) pour ces espèces, en vue des applications atmosphériques. Cependant, hormis Cl₂CO, la spectroscopie de ClNO₂₂ et ClONO₂ est rendue difficile par leur synthèse chimique très complexe, leur réactivité avec les métaux et les matériaux organiques et leur instabilité en présence de lumière et chaleur. De plus, ces molécules sont assez lourdes (présence du chlore avec ses deux isotopomères) et présentent des spectres denses et assez compliqués par de nombreuses perturbations qui affectent les niveaux de vibration-rotation.Dans le cas de ClNO₂, des spectres ont été enregistrés dans la gamme 300 – 900 cm-1 avec de meilleures conditions expérimentales (haute résolution, basse température, long parcours, faible pression) en utilisant le rayonnement synchrotron de la ligne AILES à SOLEIL. Des modélisations précises des régions à 370 et 790 cm-1 ont été effectuées. Ces régions pourront être exploitées pour une future télédétection atmosphérique par spectroscopie IRTF respectivement par les instruments FORUM et IASI-NG. Les vibrations de basse énergie de ClONO₂ jamais observées à haute résolution avant ce travail ont également été mesurées. Une première modélisation de la région de la torsion vers 120 cm-1 est présentée dans cette thèse. L’analyse de ces vibrations sera utile pour modéliser les bandes chaudes dans les fenêtres atmosphériques où ClONO₂ est actuellement détecté, et in fine permettra d’aboutir à une restitution bien plus précise du profil de concentration de ClONO₂. Concernant Cl₂CO, des sections efficaces ont été mesurées au LISA, d’une part, à température ambiante pour clarifier les données des travaux antérieurs et, d’autre part, dans les conditions stratosphériques en soutien à la télédétection satellitaire
Measuring concentrations of trace species that may have a significant impact on health, climate or the stability of the ozone layer, is a serious challenge. Future space missions, planned at high sensitivity, will bring progress if and only if the necessary spectral parameters are available. For some species of atmospheric interest such as nitryl chloride (ClNO₂), phosgene (Cl₂CO) and chlorine nitrate (ClONO₂), spectroscopic data are incomplete or almost non-existent. The challenge in this thesis is to get spectroscopic parameters (line positions and intensities or absorption cross sections) for these species in support of atmospheric applications. However, apart from Cl₂CO, spectroscopy of ClNO₂ and ClONO₂ is made difficult by their very complicated chemical synthesis, their reactivity with metals and organic materials, and their instability in the presence of light and heat. Moreover, these molecules are quite heavy (presence of chlorine with its two isotopomers) and exhibit dense spectra that are quite complicated by numerous perturbations affecting vibration-rotation levels.In the case of ClNO₂, spectra were recorded in the range 300 – 900 cm-1 with much improved experimental conditions (high resolution, low temperature, long path, low pressure) using the synchrotron radiation of the AILES beamline at SOLEIL. Precise modelling of the 370 and 790 cm-1 regions has been performed. These regions could be used for a future atmospheric remote sensing by FTIR spectroscopy respectively by FORUM and IASI-NG instruments. The low energy vibrations of ClONO₂ that have been never observed at high resolution before this work were also measured. A first modelling of the torsional region around 120 cm-1 is presented in this thesis. The analysis of these vibrations will be useful for the modelling of hot bands in the atmospheric windows where ClONO₂ is currently detected, and in fine will lead to a much more precise retrieval of the ClONO₂ concentration profile. Regarding Cl₂CO, cross sections have been measured at LISA, on one hand, at room temperature to compare data with previous works and, on the other, in stratospheric conditions to support satellite remote sensing applications

Styles APA, Harvard, Vancouver, ISO, etc.

Plus de sources

Livres sur le sujet "Synchrotron Radiation Infrared Microspectroscopy"

Moss, David, dir. Biomedical Applications of Synchrotron Infrared Microspectroscopy. Cambridge : Royal Society of Chemistry, 2010. http://dx.doi.org/10.1039/9781849731997.

Texte intégral

Styles APA, Harvard, Vancouver, ISO, etc.

1954-, Carr G. Lawrence, Dumas Paul et Society of Photo-optical Instrumentation Engineers., dir. Accelerator-based sources of infrared and spectroscopic applications : 19-20 July, 1999, Denver, Colorado. Bellingham, Wash., USA : SPIE, 1999.

Trouver le texte intégral

Styles APA, Harvard, Vancouver, ISO, etc.

1946-, Williams Gwyn P., Dumas Paul et Society of Photo-optical Instrumentation Engineers., dir. Accelerator-based infrared sources and applications : 29-30 July 1997, San Diego, California. Bellingham, Wash : SPIE, 1997.

Trouver le texte intégral

Styles APA, Harvard, Vancouver, ISO, etc.

Biomedical Application Of Synchotron Infrared Microspectroscopy. Royal Society of Chemistry, 2010.

Trouver le texte intégral

Styles APA, Harvard, Vancouver, ISO, etc.

(Editor), P. Rullhusen, X. Artru (Editor) et Pierre Dhez (Editor), dir. Novel Radiation Sources Using Relativistic Electrons, from Infrared to X-Rays : F Rom Infrared to X-Rays (Series on Synchrotron Radiation Techniques and Applications, Vol. 4). World Scientific Pub Co Inc, 1997.

Trouver le texte intégral

Styles APA, Harvard, Vancouver, ISO, etc.

Meyers, Robert A. Encyclopedia of Molecular Cell Biology and Molecular Medicine, Sex Hormones (Male) : Analogs and Antagonists to Synchrotron Infrared Microspectroscopy (Encyclopedia ... Biology and Molecular Medicine 16Vset). 2^e éd. Wiley-VCH, 2005.

Trouver le texte intégral

Styles APA, Harvard, Vancouver, ISO, etc.

Chapitres de livres sur le sujet "Synchrotron Radiation Infrared Microspectroscopy"

Reffner, John A., G. Lawrence Carr et Gwyn P. Williams. « Infrared Microspectroscopy with Synchrotron Radiation ». Dans Progress in Fourier Transform Spectroscopy, 339–41. Vienna : Springer Vienna, 1997. http://dx.doi.org/10.1007/978-3-7091-6840-0_76.

Texte intégral

Styles APA, Harvard, Vancouver, ISO, etc.

Perucchi, Andrea, Lisa Vaccari et Stefano Lupi. « Infrared Synchrotron Radiation : From the Production to the Scientific Applications ». Dans Synchrotron Radiation, 437–60. Berlin, Heidelberg : Springer Berlin Heidelberg, 2014. http://dx.doi.org/10.1007/978-3-642-55315-8_15.

Texte intégral

Styles APA, Harvard, Vancouver, ISO, etc.

Miller, Lisa M., et Paul Dumas. « Infrared Spectroscopy Using Synchrotron Radiation ». Dans Encyclopedia of Biophysics, 1–9. Berlin, Heidelberg : Springer Berlin Heidelberg, 2018. http://dx.doi.org/10.1007/978-3-642-35943-9_128-1.

Texte intégral

Styles APA, Harvard, Vancouver, ISO, etc.

Miller, Lisa M., et Paul Dumas. « Infrared Spectroscopy using Synchrotron Radiation ». Dans Encyclopedia of Biophysics, 1106–12. Berlin, Heidelberg : Springer Berlin Heidelberg, 2013. http://dx.doi.org/10.1007/978-3-642-16712-6_128.

Texte intégral

Styles APA, Harvard, Vancouver, ISO, etc.

Russell, Andrea E., et William O’Grady. « Far-Infrared Synchrotron Radiation and the Electrochemical Interface ». Dans Synchrotron Techniques in Interfacial Electrochemistry, 421–31. Dordrecht : Springer Netherlands, 1994. http://dx.doi.org/10.1007/978-94-017-3200-0_24.

Texte intégral

Styles APA, Harvard, Vancouver, ISO, etc.

Hoffmann, F. M., et G. P. Williams. « Synchrotron Radiation in the Far Infrared : Infrared Reflection Absorption Spectroscopy ». Dans Springer Series in Surface Sciences, 263–85. Berlin, Heidelberg : Springer Berlin Heidelberg, 1995. http://dx.doi.org/10.1007/978-3-642-79024-9_7.

Texte intégral

Styles APA, Harvard, Vancouver, ISO, etc.

Kimura, Shin-ichi. « Infrared and Terahertz Synchrotron Radiation : Optics and Applications ». Dans Springer Series in Optical Sciences, 63–81. Berlin, Heidelberg : Springer Berlin Heidelberg, 2013. http://dx.doi.org/10.1007/978-3-642-40594-5_4.

Texte intégral

Styles APA, Harvard, Vancouver, ISO, etc.

Mathis, Y.-L., K. Murakoshi, A. Tadjeddine et P. Roy. « Fourier Transform Infrared Combined with Synchrotron Radiation for Probing the Electrochemical Interface ». Dans Synchrotron Techniques in Interfacial Electrochemistry, 401–20. Dordrecht : Springer Netherlands, 1994. http://dx.doi.org/10.1007/978-94-017-3200-0_23.

Texte intégral

Styles APA, Harvard, Vancouver, ISO, etc.

Hirschmugl, Carol J., et Gwyn P. Williams. « The Application of Infrared Synchrotron Radiation to the Study of Interfacial Vibrational Modes ». Dans Synchrotron Techniques in Interfacial Electrochemistry, 387–99. Dordrecht : Springer Netherlands, 1994. http://dx.doi.org/10.1007/978-94-017-3200-0_22.

Texte intégral

Styles APA, Harvard, Vancouver, ISO, etc.

Mattson, Eric C., Miriam Unger, Julia Sedlmair, Michael Nasse, Ebrahim Aboualizadeh, Zahrasadat Alavi et Carol J. Hirschmugl. « Widefield FT-IR 2D and 3D Imaging at the Microscale Using Synchrotron Radiation ». Dans Infrared and Raman Spectroscopic Imaging, 585–618. Weinheim, Germany : Wiley-VCH Verlag GmbH & Co. KGaA, 2014. http://dx.doi.org/10.1002/9783527678136.ch15.

Texte intégral

Styles APA, Harvard, Vancouver, ISO, etc.

Actes de conférences sur le sujet "Synchrotron Radiation Infrared Microspectroscopy"

Morikawa, E. « Infrared Microspectroscopy Beamline at CAMD ». Dans SYNCHROTRON RADIATION INSTRUMENTATION : Eighth International Conference on Synchrotron Radiation Instrumentation. AIP, 2004. http://dx.doi.org/10.1063/1.1757809.

Texte intégral

Styles APA, Harvard, Vancouver, ISO, etc.

Kizilkaya, O., V. Singh, Y. Desta, M. Pease, A. Roy, J. Scott, J. Goettert, E. Morikawa, J. Hormes et A. Prange. « The Infrared Microspectroscopy Beamline at CAMD ». Dans SYNCHROTRON RADIATION INSTRUMENTATION : Ninth International Conference on Synchrotron Radiation Instrumentation. AIP, 2007. http://dx.doi.org/10.1063/1.2436409.

Texte intégral

Styles APA, Harvard, Vancouver, ISO, etc.

Carr, G. Lawrence, et Gwyn P. Williams. « Infrared microspectroscopy with synchrotron radiation ». Dans Optical Science, Engineering and Instrumentation '97, sous la direction de Gwyn P. Williams et Paul Dumas. SPIE, 1997. http://dx.doi.org/10.1117/12.290262.

Texte intégral

Styles APA, Harvard, Vancouver, ISO, etc.

Kimura, Shin-ichi, Yoko Sakurai, Eiken Nakamura et Takafumi Mizuno. « Terahertz Microspectroscopy and Infrared Reflection-Absorption Spectroscopy Apparatuses at UVSOR-II ». Dans SYNCHROTRON RADIATION INSTRUMENTATION : Ninth International Conference on Synchrotron Radiation Instrumentation. AIP, 2007. http://dx.doi.org/10.1063/1.2436131.

Texte intégral

Styles APA, Harvard, Vancouver, ISO, etc.

Miller, Lisa M. « Biological infrared microspectroscopy at the National Synchrotron Light Source ». Dans The 11th US national synchrotron radiation instrumentation conference (SRI99). AIP, 2000. http://dx.doi.org/10.1063/1.1291757.

Texte intégral

Styles APA, Harvard, Vancouver, ISO, etc.

BOCCI, ALESSIO, EMANUELE M. PACE, AUGUSTO MARCELLI, PIERANGELO MORINI et DIEGO SALI. « OPTIMIZED INFRARED DETECTORS FOR INFRARED SYNCHROTRON RADIATION MICROSPECTROSCOPY AND BIOMEDICAL IMAGING ». Dans Proceedings of the 9th Conference. WORLD SCIENTIFIC, 2006. http://dx.doi.org/10.1142/9789812773678_0106.

Texte intégral

Styles APA, Harvard, Vancouver, ISO, etc.

Cinque, Gianfelice. « The synchrotron radiation IR microspectroscopy facility at Diamond Light Source (Invited Keynote) ». Dans 2007 Joint 32nd International Conference on Infrared and Millimeter Waves and the 15th International Conference on Terahertz Electronics (IRMMW-THz). IEEE, 2007. http://dx.doi.org/10.1109/icimw.2007.4516656.

Texte intégral

Styles APA, Harvard, Vancouver, ISO, etc.

Polito, Raffaella, Valeria Giliberti, Maria Eleonora Temperini, Eglof Ritter, Matthias Broser, Peter Hegemann, Ljiljana Puskar, Ulrich Schade, Leonetta Baldassarre et Michele Ortolani. « Light-induced conformational changes of two different Channelrhodopsin mutants probed by difference mid-Infrared microspectroscopy with Synchrotron radiation ». Dans 2020 45th International Conference on Infrared, Millimeter and Terahertz Waves (IRMMW-THz). IEEE, 2020. http://dx.doi.org/10.1109/irmmw-thz46771.2020.9370989.

Texte intégral

Styles APA, Harvard, Vancouver, ISO, etc.

Paterson, D. J., J. W. Boldeman, D. D. Cohen et C. G. Ryan. « Microspectroscopy Beamline at the Australian Synchrotron ». Dans SYNCHROTRON RADIATION INSTRUMENTATION : Ninth International Conference on Synchrotron Radiation Instrumentation. AIP, 2007. http://dx.doi.org/10.1063/1.2436197.

Texte intégral

Styles APA, Harvard, Vancouver, ISO, etc.

Rivers, Mark L., Stephen R. Sutton et Sasa Bajt. « X-ray microspectroscopy using synchrotron radiation ». Dans OSA Annual Meeting. Washington, D.C. : Optica Publishing Group, 1993. http://dx.doi.org/10.1364/oam.1993.fbb.3.

Texte intégral

Résumé :

The synchrotron x-ray fluorescence microprobe at bending magnet beamline X-26A at the National Synchrotron Light Source has been used for a number of years for trace element microanalysis using collimated white radiation.

Styles APA, Harvard, Vancouver, ISO, etc.

Nous offrons des réductions sur tous les plans premium pour les auteurs dont les œuvres sont incluses dans des sélections littéraires thématiques. Contactez-nous pour obtenir un code promo unique!