Thèses sur le sujet « Supervision en ligne des bioprocédés »
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Yang, Ning. « Online monitoring of bioreactors by Raman spectroscopy and machine learning ». Electronic Thesis or Diss., université Paris-Saclay, 2024. http://www.theses.fr/2024UPAST083.
Texte intégralThis thesis presents a novel and reproducible modeling strategy for online monitoring of bioreactors using Raman spectroscopy and Machine Learning. The main aim of this study is to develop simplified models using the Raman spectra of standards in solution. It consists of three key parts.The first part involves optimizing Raman acquisition parameters and developing a PLS regression model using pure standards. Subsequently, a preliminary validation was carried out using mixed standards to mimic changes in the composition of different molecules in the medium during the alcoholic fermentation process. The second part defined a nonlinear expression to interpret the Raman attenuation induced by the presence of microorganisms in a real bioreactor, allowing the correction of cell--scattered spectra. For model evaluation, numerous batches and one fed--batch bioreactor were launched to validate the working performance and predictive robustness of the obtained correction strategy and regression model. The third part highlights the advantages of our proposed modeling methodology over the traditional way that uses the spectra from bioreactors to train the regression model.Overall, the innovative approach demonstrated an excellent prediction performance on all validation and testing datasets, presenting significant potential for bioprocess engineering. It enables more accurate and efficient monitoring of multiple compounds in real time, as well as enhances process control and optimization. The proposed strategy is expected to have an extended application in the bioproduction industry
Kabbaj, Mohammed Nabil. « Développement d'algorithmes de détection et d'isolation de défauts pour la supervision des bioprocédés ». Perpignan, 2004. http://www.theses.fr/2005PERP0599.
Texte intégralBaldellon, Olivier. « Supervision en ligne de propriétés temporelles dans les systèmes distribués temps-réel ». Phd thesis, Toulouse, INPT, 2014. http://oatao.univ-toulouse.fr/13299/1/baldellon.pdf.
Texte intégralWu, Chang. « Dispositif microfluidique utilisant la technologie d’électromouillage sur isolant dédié à la préparation d’échantillons pour des analyses biologiques : application au suivi en ligne de bioprocédés ». Thesis, Lille 1, 2012. http://www.theses.fr/2012LIL10173/document.
Texte intégralThis work presents the concept, fabrication technology and characterization of a sample preparation unit using an original approach coupling channel-based continuous and electrowetting-on-dielectric (EWOD)-based digital microfluidics. The major advantage of ‘digital’ is the accurate control of multiple reagents without the need of a complex network of microvalves, while unprocessed and reprocessed ‘continuous’ format is ideal for coupling with upstream and downstream microfluidic devices. We have developed two generations. In our first work, a three layers PSP (Pyrex-Silicon-Pyrex) configuration with hydrophobic liquid-solid interfaces was employed. An original adhesive wafer bonding technique has been optimized that is sufficiently generic to be used in diverse MEMS processes. However, the preliminary characterization results have shown that most real samples used in bioprocessing could not be handled by this first prototype. To address this issue, we have developed a bilayer PS (Pyrex-Silicon) configuration with superhydrophobic liquid-solid interfaces made by chemical nanotexturation of silicon. Thanks to the low contact angle hysteresis of this superhydrophobic surface, the friction resistance and bio-adsorption on the surface were largely reduced allowing transport of real complex liquids. Finally, this prototype has been successfully used for preconditioning samples taken from a yeast bio-reactor and then delivered to analytical modules either an enzyme-linked immunosorbent assay (ELISA) or a capillary electrophoresis (CE) device coupled with a mass spectrometry (MS)
Atine, Jean-Charles. « Méthodes d'apprentissage flou : application à la segmentation d'images biologiques ». Toulouse, INSA, 2005. http://eprint.insa-toulouse.fr/archive/00000272/.
Texte intégralThe presented works have for objective to help the biologists in the diagnosis of the cellular viability by using some methods of classification. Our work announces a strategy of classification allowing to building partition of images of cells coming from an optical microscope. We classify automatically the cells by operating the segmentation on images using the developed algorithm T-LAMDA. A statement concerning the existing classification methods, the color space and the resistance to noise, allows to finding the structure the most adapted to our study. The comparative analysis of various methods (of which LAMDA and T-LAMDA methods), allows us to put in evidence the most appropriate for the classification of cells subjected to the blue of methylene solution. We propose some supervised algorithms based on LAMDA to show if the way of treating the data influence the result. The T-LAMDA algorithm, based on the decision trees, shows itself the best adapted for our study and so gives more precise results than other methods, with a shorter time of execution. We suggest learning by using the CELCA application, Cell Classification Application, which uses the developed T-LAMDA algorithm. The software takes care of calculations of the kinetics, according to the images which respect to a well defined protocol. Time for treating 117 images is 6 '47'' minutes, what is widely below the time taken by biologists to count the cells
Roth, Sandrine. « Diagnostic d'une installation sidérurgique complexe : analyse de la ligne et proposition d'une architecture pour un système de diagnostic-supervision dédié ». Nancy 1, 1995. http://www.theses.fr/1995NAN10430.
Texte intégralThe subject of this study is about a continuous galvanising line. The general objective of this work is the realisation of a default detection, diagnosis and operators assistance system. That kind of system is intended to the control and to the maintenance of the Sollac complex plant, in order to improve the production and the product quality and the productivity. At first, we have searched any interesting information about the galvanising line, to understand the functioning of each processes which are involved in the production of galvanised iron sheet. Then, the bibliographical analysis of knowledged based diagnosis methods (neural networks, expert systems, fuzzy logic, qualitative physics) is writen to find a strategy to supervise such a non linear multi-variable system. The model based methods are useless because of the lack of available models. The next stage concerned the mesurements collections. The measurements analysis results allow us to build a knowledge base with some defaults trees (defaults, causes and effects) and treatment rules. Then the supervision system architecture is defined. A multi-agent system is described to realise the whole monitoring system and a structure based on an object model is proposed to implement that system
Paquereau, Delphine. « Gestion de procédures et prise en compte du danger lors de l'occurrence d'incidents combinés : Application à la supervision d'une ligne de métro ». Thesis, Lyon, INSA, 2015. http://www.theses.fr/2015ISAL0024/document.
Texte intégralDuring metro line operations, the supervision operator is responsible for the procedures’ execution when referring to incidents management. However, when combined incidents occur, procedures may be competing. In this particular case, situations which do not ensure people’s safety exist and an accident might happen. Firstly, the approach of studying these procedures integrates their graphical representation with the BPMN notation. These procedures’ models, understandable and accessible, provide a significant amount of knowledge for industrials in this area. Secondly, these models are performed as Petri nets to add dynamic to the system of interest. That is why, the notion of controllability and the influence of the execution context are introduced in the study of incidents management procedures. To ensure people’s safety, forbidden states are defined and identified among the states space with the supervisory control theory. These states are characterized in an original way : depending on their inclusion in a set of states but also depending on the controllability of their outgoing transitions. In addition to this innovative characterization, algorithms allow to determine and to avoid forbidden states. Criteria distinguish the admissible sequences which avoid forbidden states. These differentiation criteria are defined to steer the supervision operator through the actions he has to execute when combined incidents occur. Results allow us to provide assistance to the supervision operator with warnings and advice. This study is based on ATS supervision system developed by Thales and one of their customers’ incidents management procedures, the RATP. A prototype of operator support functionality for incidents management based on customer know-how has been implemented into Thales software
Abdo, Diala. « Modélisation dynamique et supervision des fours de réchauffage continus de la sidérurgie ». Phd thesis, École Nationale Supérieure des Mines de Paris, 2006. http://pastel.archives-ouvertes.fr/pastel-00002236.
Texte intégralSayed-Mouchaweh, Moamar. « Conception d'un système de diagnostic adaptatif et prédictif basé sur la méthode Fuzzy Pattern Matching pour la surveillance en ligne des sytèmes évolutifs : Application à la supervision et au diagnostic d'une ligne de peinture au trempé ». Reims, 2002. http://www.theses.fr/2002REIMS018.
Texte intégralLi, Mengyao. « Approche méthodologique innovante pour le suivi en ligne de procédés de production d’anticorps par cellules animales : apport des techniques spectroscopiques in situ à la stratégie PAT ». Thesis, Université de Lorraine, 2018. http://www.theses.fr/2018LORR0151/document.
Texte intégralBioprocesses of mammalian cell culture have become essential for the production of therapeutic recombinant proteins, such as monoclonal antibodies (mAb). However, the physiological state of the cells and the quality of the mAb produced, in particular their glycosylation, may vary during the process, and may lead to the alteration of the safety and efficacy of the final product. Consequently, the Process Analytical Technology (PAT) initiative has encouraged the development of online monitoring techniques, with the aim to better control the process and ensure the quality of the final product. In this context, this thesis proposes innovative approaches for online monitoring of CHO (Chinese Hamster Ovary) cells bioreactor cultures, by using three types of in situ spectroscopic measurements (dielectric, Raman, near infrared (NIR)). The first chapter presents a novel approach to predict in real-time one of the major cell physiological state parameters, the specific growth rate (µ). Based on online permittivity measured by in situ dielectric spectroscopy, the cell concentration was estimated and µ was calculated in real-time, making possible to detect the critical moment when µ begins to decrease significantly. Compared to an offline approach, this online approach allowed to maintain the cells in a stable physiological state, ensuring the glycosylation of the mAb produced in feed-harvest cultures. The second chapter shows the use of in situ NIR and Raman spectroscopies combined with chemometric methods. For the first time, the performances of these two spectroscopies were compared in parallel in the same cultures. Online models were developed to predict in real-time the concentration of different parameters (viable cells, glucose, lactate, glutamine, ammonium ions and antibodies). The evaluation of these models by the multivariate Figures of Merit (FOM) revealed some of the advantages of Raman spectroscopy. The combination of the two spectroscopies by various data fusion strategies has also been evaluated. In the third chapter, the interest of Raman spectroscopy for the online monitoring of both the quantity and the glycosylation of the mAb was demonstrated. Models were developed for online prediction of both macroheterogeneity (glycosylation site occupancy) and microheterogeneity (glycan structures) of mAb glycosylation in batch and feed-harvest cultures. The last chapter used models previously developed for NIR and dielectric spectroscopies, to integrate into a “soft sensor” by combining with cell metabolic and mass balance equations. This “soft sensor”, implemented in a fed-batch cell culture for the automatic control of the feed rate, leads to an increased mAb productivity and better mAb glycosylation
Mrani, Alaoui Rim. « Conception d'un module de diagnostic à base de suites de bandes temporelles en vue de la supervision des procédés énergétiques : application en ligne à un générateur de vapeur ». Lille 1, 2004. http://www.theses.fr/2004LIL10096.
Texte intégralLi, Mengyao. « Approche méthodologique innovante pour le suivi en ligne de procédés de production d’anticorps par cellules animales : apport des techniques spectroscopiques in situ à la stratégie PAT ». Electronic Thesis or Diss., Université de Lorraine, 2018. http://www.theses.fr/2018LORR0151.
Texte intégralBioprocesses of mammalian cell culture have become essential for the production of therapeutic recombinant proteins, such as monoclonal antibodies (mAb). However, the physiological state of the cells and the quality of the mAb produced, in particular their glycosylation, may vary during the process, and may lead to the alteration of the safety and efficacy of the final product. Consequently, the Process Analytical Technology (PAT) initiative has encouraged the development of online monitoring techniques, with the aim to better control the process and ensure the quality of the final product. In this context, this thesis proposes innovative approaches for online monitoring of CHO (Chinese Hamster Ovary) cells bioreactor cultures, by using three types of in situ spectroscopic measurements (dielectric, Raman, near infrared (NIR)). The first chapter presents a novel approach to predict in real-time one of the major cell physiological state parameters, the specific growth rate (µ). Based on online permittivity measured by in situ dielectric spectroscopy, the cell concentration was estimated and µ was calculated in real-time, making possible to detect the critical moment when µ begins to decrease significantly. Compared to an offline approach, this online approach allowed to maintain the cells in a stable physiological state, ensuring the glycosylation of the mAb produced in feed-harvest cultures. The second chapter shows the use of in situ NIR and Raman spectroscopies combined with chemometric methods. For the first time, the performances of these two spectroscopies were compared in parallel in the same cultures. Online models were developed to predict in real-time the concentration of different parameters (viable cells, glucose, lactate, glutamine, ammonium ions and antibodies). The evaluation of these models by the multivariate Figures of Merit (FOM) revealed some of the advantages of Raman spectroscopy. The combination of the two spectroscopies by various data fusion strategies has also been evaluated. In the third chapter, the interest of Raman spectroscopy for the online monitoring of both the quantity and the glycosylation of the mAb was demonstrated. Models were developed for online prediction of both macroheterogeneity (glycosylation site occupancy) and microheterogeneity (glycan structures) of mAb glycosylation in batch and feed-harvest cultures. The last chapter used models previously developed for NIR and dielectric spectroscopies, to integrate into a “soft sensor” by combining with cell metabolic and mass balance equations. This “soft sensor”, implemented in a fed-batch cell culture for the automatic control of the feed rate, leads to an increased mAb productivity and better mAb glycosylation
Mirdamadi, Samieh. « Modélisation du processus de pilotage d'un atelier en temps réel à l'aide de la simulation en ligne couplée à l'exécution ». Thesis, Toulouse, INPT, 2009. http://www.theses.fr/2009INPT022G/document.
Texte intégralThis research work highlights the interest of online simulation as a decision support tool. This tool is focused on workshop control in relation with real time and short-term events. Online simulation enables to analyze and compare scenarios affecting the production, such as failures or unexpected orders. Moreover online simulation allows a very short term projection in the future in order to quantify the consequences of some unexpected event: we call it projection simulation. Finally, online simulation is helpful to choose the solution that will reduce the impact of a critical event, through the simulation of several scenarios. To conclude, online simulation is one the most interesting decision support tool in workshop piloting. However, there are few industrial applications in manufacturing systems. The first part of this research work introduces the overall context of production system management. The different generic functions of controlling the production, the modes of piloting and the evolutions of workshop are detailed. We also explain piloting typologies according to their reactivity. In the second part, we study the existing software tools used in industry for the operation of production, and support tools that ensure optimal compliance in regard to the objectives. After a preliminary work on the offline simulation, we propose to couple an online simulation tool with a type MES delivery tool in order to control the production process in real time. A modeling approach using two complementary approaches, an analytical one and simulation, is then introduced. The objective of the third part is to emphasize the use of online flow simulation to assist in operating a production process. Thus we model the process in real time to describe the required functions. Therefore we try out different strategies of use of online simulators, through one or two models and several methods of using these models. Online simulation then provides realistic information on the short-term consequences of an event, as well as on the different ways of correcting the scenarios. To convince industry of the relevance of online simulation, the fourth part presents practical solutions to the specifications and requirements previously stated. An experimental platform helped us validate the concept of online simulation as piloting support tool, but also underline the difficulties of development
SAYED, MOUCHAWEH Moamar. « Conception d'un système de diagnostic adaptatif et prédictif basé sur la méthode Fuzzy Pattern Matching pour la surveillance en ligne des systèmes évolutifs ». Phd thesis, Université de Reims - Champagne Ardenne, 2002. http://tel.archives-ouvertes.fr/tel-00002637.
Texte intégral- dans une base de connaissance incomplète, tous les modes de fonctionnement ne sont pas représentés. En conséquence, un module de diagnostic doit être adaptatif afin d'inclure à sa base de connaissance les nouveaux modes dés qu'ils apparaissent,
- lorsque le système évolue vers un mode anormal ou non désiré, il est nécessaire d'anticiper cette évolution plutôt que d'attendre d'arriver à ce mode afin d'éviter ses conséquences surtout s'il est dangereux. Le module de diagnostic doit donc être prédictif,
- dans le cas d'un système évolutif, la base de connaissance doit être enrichie grâce à l'information apportée par les nouvelles observations. Cet enrichissement doit être réalisé en temps réel,
- les données sont à la fois incertaines et imprécises.
L'objectif principal de ma thèse consistait à mettre au point un module de diagnostic en temps réel adaptatif et prédictif pour des systèmes évolutifs, en utilisant les techniques de Reconnaissance des Formes, la théorie des ensembles flous et la théorie des possibilités. Ce module a été appliqué sur plusieurs applications industrielles.
Lefort, Romain. « Contribution des technologies CPL et sans fil à la supervision des réseaux de distribution d'électricité ». Thesis, Poitiers, 2015. http://www.theses.fr/2015POIT2253/document.
Texte intégralEstablishing a supervisory infrastructure allows a better smart management than an expensive strengthening of distribution network to respond to new constraints at the energies control (Consumption, REN, EV ...). To transmit data, Power Line Communication (PLC) technologies present an advantage in this context. In fact, it enables a superposition of High Frequency (HF) signals on electrical signal 50/60 Hz. However, electric networks have not been developed to this application because of difficult propagation conditions. This research work makes a contribution to develop a simulation platform in objective to transmit data to 1 MHz. In first time, each network element is studied singly and in second time, together, to estimate "Outdoor PLC" transmission performance. The first element studied is the networks variation in function of frequency and time. Several 24h disturbance measurements on LV customers are presented. The second element is the transformers which established connection between Medium Voltage (MV) and Low Voltage (LV). The proposed modeling method is based on a "lumped model" and a "black box model". These models are applied to a 100 kVA H61 transformer most commonly used by French distribution system operator in rural and suburban networks. The third element is the power line used in MV and LV networks. The proposed modeling method is based on a "cascaded model" from the theory of transmission line. This model is applied to one power line used in LV underground network. Each model is obtained from various impedance measurements. To complete, an introductory study on mobile radio communication is performed to remote network distribution
Manyri, Laurent. « Analyse automatique d'images de populations microbiennes ». Phd thesis, INSA de Toulouse, 2005. http://tel.archives-ouvertes.fr/tel-00011336.
Texte intégralChouinard, Véronique. « Le soutien au développement de la pratique des infirmières praticiennes spécialisées de première ligne au Québec ». Thèse, 2014. http://hdl.handle.net/1866/11936.
Texte intégralAlthough there is an abundant literature on primary health care nurse practitioners (PHCNP), the optimal structures and practices to support PHCNPs remain largely unexplored. In Quebec, this issue needs particular attention because of practice settings diversity and physical distance between major key players. Furthermore, the supervision role that seems to be shared by multiple professionals and the legal framework add to the complexity of the implementation of support structures and practices. Through a multiple case study, this paper aimed to explore the managerial structures in place and to analyze the mechanisms deployed to support the development of PHCNP practice. Issuing from a larger scale multicenter research, this study analyzed three separate cases, totaling 18 interviews with health professionals and managers. Based on a three levels model built on the central concept of support, the needs of PHCNP and their teams have been identified. The main results of this research demonstrate the relevance of supervision by the key players of the Department of Nursing. In addition, the coordination between professionals seems enhanced when some organizational structures are implemented. Finally, decision-making committees linked to the implementation of PHCNP appear to be more useful when the environment and experience of the organizations are taken into consideration.