Thèses sur le sujet « Substrate identification »
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Thomas, Daniel Alexander. « Application of peptide and cDNA libraries to protease substrate identification ». Thesis, University of Leeds, 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.418926.
Texte intégralRedbird, Ruth Ann. « Identification of a protein kinase substrate in Sulfolobus solfataricus P2 ». Diss., Virginia Tech, 2010. http://hdl.handle.net/10919/26884.
Texte intégralPh. D.
Zhang, Peng. « Functional Characterization of Protein Tyrosine Phosphatases in Tumorigenesis through Substrate Identification ». Case Western Reserve University School of Graduate Studies / OhioLINK, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=case1365174835.
Texte intégralCampbell, Timothy. « Methods for Arrhythmogenic Substrate Identification and Procedural Improvements for Ventricular Arrhythmias ». Thesis, The University of Sydney, 2022. https://hdl.handle.net/2123/29925.
Texte intégralChin, Wing Hong. « Identification of TrkB as a p35 interacting protein and a Cdk5 substrate / ». View abstract or full-text, 2005. http://library.ust.hk/cgi/db/thesis.pl?BICH%202005%20CHIN.
Texte intégralHumphreys, D. « Identification of a novel substrate of the Salmonella protein tyrosine phosphatase SptP ». Thesis, University of Cambridge, 2007. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.604780.
Texte intégralKusevic, Denis [Verfasser], et Albert [Akademischer Betreuer] Jeltsch. « Biochemical investigation of the substrate specificity of protein methyltransferases and the identification of novel substrates / Denis Kusevic ; Betreuer : Albert Jeltsch ». Stuttgart : Universitätsbibliothek der Universität Stuttgart, 2016. http://d-nb.info/1165574489/34.
Texte intégralBoswell, Nicholas William Bradford. « Biochemical characterization of the [FeFe]-hydrogenase maturation protein HydE and identification of the substrate ». Thesis, Montana State University, 2011. http://etd.lib.montana.edu/etd/2011/boswell/BoswellN1211.pdf.
Texte intégralFarah, Sahar. « Identification of Rho-associated protein kinaseà as an insulin receptor substrate-1 binding protein ». Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp01/MQ28422.pdf.
Texte intégralCheng, Kai. « Identification of Pctaire1 as a p35-interacting protein and a novel substrate for Cdk5 / ». View Abstract or Full-Text, 2003. http://library.ust.hk/cgi/db/thesis.pl?BICH%202003%20CHENG.
Texte intégralIncludes bibliographical references (leaves 153-177). Also available in electronic version. Access restricted to campus users.
Farah, Sahar. « Identification of Rho-associated protein kinase-alpha as an insulin receptor substrate-1 binding protein ». Thesis, University of Ottawa (Canada), 1998. http://hdl.handle.net/10393/4152.
Texte intégralMueller, Martin J. « Leukotriene A₄ hydrolase : identification of amino acid residues involved in catalyses and substrate-mediated inactivation / ». Stockholm, 2001. http://diss.kib.ki.se/2001/91-628-4934-4/.
Texte intégralBerglund, Frederick M. « Identification of hnRNP-U as a DNA-PK substrate phosphorylated in response to DNA damage ». Thesis, University of Dundee, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.505651.
Texte intégralCole, Kathryn O. « Printability and environmental testing using silver-based conductive flexographic ink printed on a polyamide substrate / ». Online version of thesis, 2007. http://hdl.handle.net/1850/4490.
Texte intégralKahl, Philipp. « Identification of long-range solid-like correlations in liquids and role of the interaction fluid-substrate ». Thesis, Le Mans, 2016. http://www.theses.fr/2016LEMA1002/document.
Texte intégralLiquids differ from solids by a delayed response to a shear mechanical solicitation; i.e. they have no shearelasticity and exhibit a flow behaviour at low frequency (<1 Hz). This postulate might be not verified at thesub-millimeter scale. By optimizing the measurement in particular by improving the liquid/substrate interactions (wetting), a low frequency shear elasticity has been found in liquids including molten polymers, glass-formers, H-bond polar, ionic or van der Waals liquids. This result implies that molecules in the liquid state may not be dynamically free but weaklyelastically correlated. Using the birefringent properties of the pretransitional fluctuations coexisting in the isotropic phase of liquid crystals, we show that it is possible to visualize these “hidden” shear-elastic correlations. We detect a synchronized birefringent optical response in the isotropic phase that is observable at frequencies as low as 0.01 Hz and at temperatures far away from anyphase transition. The low-frequency birefringence exhibits a strain dependence similar to the low frequency elasticity: An optical signal that is in-phase with the strain at low strain amplitudes and in-phase with the strain-rate at larger strain amplitudes. The birefringent response is strong, defect-free, reversible and points out a collective response. This long-range ordering rules out the condition of an isotropic liquid and its synchronized response supports the existenceof long-range elastic (solid-like) correlations. In the light of this, the strain dependence of the harmonic birefringent signal and the shear elasticity may correspond to an entropy-driven transition
Cowan, Jon Walter. « Proteolysis and the growth hormone receptor identification and characterization of GHR as a [gamma]-secretase substrate / ». Thesis, Birmingham, Ala. : University of Alabama at Birmingham, 2007. https://www.mhsl.uab.edu/dt/2009r/cowan.pdf.
Texte intégralMyers, Alexandra. « Identification of CaMK-II Protein Targets in Tissue Culture and Zebrafish Embryos using Tandem Mass Spectrometry ». VCU Scholars Compass, 2009. http://scholarscompass.vcu.edu/etd/10.
Texte intégralKenny, Thomas Donald. « Identification of High-Velocity Pseudo-surface Acoustic Wave Substrate Orientations and Modeling of Surface Acoustic Wave Structures ». Fogler Library, University of Maine, 2011. http://www.library.umaine.edu/theses/pdf/KennyT2011.pdf.
Texte intégralNguyen, Hue Bach Thi [Verfasser], et Wolfgang [Akademischer Betreuer] Schumann. « Identification of substrate proteins of FtsH during sporulation of Bacillus subtilis / Hue Bach Thi Nguyen. Betreuer : Wolfgang Schumann ». Bayreuth : Universitätsbibliothek Bayreuth, 2012. http://d-nb.info/1023305542/34.
Texte intégralSathanantham, Preethi, Shiva K. Devaiah et Cecelia A. McIntosh. « Structure-Function Analysis of Grapefruit Glucosyltransferase Protein – Identification of Key Amino Acid Residues for its Rigid Substrate Specificity ». Digital Commons @ East Tennessee State University, 2015. https://dc.etsu.edu/etsu-works/352.
Texte intégralHerdendorf, Timothy J. Miziorko Henry M. « Phosphomevalonate kinase investigation of the recombinant human enzyme and identification of key residues involved in substrate binding and catalysis / ». Diss., UMK access, 2007.
Trouver le texte intégral"A dissertation in molecular biology and biochemistry and cell biology and biophysics." Advisor: Henry M. Miziorko. Typescript. Vita. Title from "catalog record" of the print edition Description based on contents viewed Sept. 12, 2008. Includes bibliographical references (leaves 109-126). Online version of the print edition.
Yang, Li. « Design and development of novel radio frequency identification (RFID) tag structures ». Diss., Atlanta, Ga. : Georgia Institute of Technology, 2009. http://hdl.handle.net/1853/31824.
Texte intégralCommittee Chair: Tentzeris, Manos; Committee Member: DeJean, Gerald; Committee Member: Ingram, Mary; Committee Member: Kavadias, Stylianos; Committee Member: Laskar, Joy. Part of the SMARTech Electronic Thesis and Dissertation Collection.
Ferguson, Alexandra. « Synthesis and ring-opening of NH-aziridine-2-carboxylates, and preparation of novel pyrazolo[3,4-d]pyrimidines for kinase-substrate identification ». Thesis, Imperial College London, 2013. http://hdl.handle.net/10044/1/11074.
Texte intégralHammes, Clay. « Identification and analysis of upper extremity cumulative trauma disorders in the substrate disk polishing areas of ABC Company in central Minnesota ». Online version, 1999. http://www.uwstout.edu/lib/thesis/1999/1999hammesc.pdf.
Texte intégralWegner, Nina Vanessa [Verfasser], Jens C. [Akademischer Betreuer] Brüning et Günter [Akademischer Betreuer] Schwarz. « Identification of in vivo interactions of insulin receptor substrate 1 in murine liver / Nina Vanessa Wegner. Gutachter : Jens C. Brüning ; Günter Schwarz ». Köln : Universitäts- und Stadtbibliothek Köln, 2011. http://d-nb.info/1038168716/34.
Texte intégralMoheshwarnath, Issur. « Structural definition of substrate recognition by model RNA capping enzymes and the identification of a novel class of viral RNA capping enzymes ». Thèse, Université de Sherbrooke, 2011. http://savoirs.usherbrooke.ca/handle/11143/4315.
Texte intégralDavid, Marion. « Identification and functional characterization of an ABC transporter of Haemonchus contortus, the P-glycoprotein 13 ». Thesis, Toulouse 3, 2016. http://www.theses.fr/2016TOU30206/document.
Texte intégralMacrocyclic lactones (ML) are paralyzing anthelmintics used in animals and humans against parasite nematodes. However, their therapeutic success is compromised by the spread of ML resistance. This might be at least partly due to P-glycoproteins (Pgps) ABC transporters that are selected and overexpressed in ML-resistant nematodes. Deciphering the role of the 10 Pgps expressed in the parasite of small ruminants Haemonchus contortus is thus of major importance to guaranty anthelmintic (AH) efficacy of various drugs. Here we focused on Hco-Pgp-13 due to the expression in the amphids of its closest ortholog in the model nematode C. elegans. Indeed, the amphids represent a putative entry route of drugs to reach AH targets in the nervous system and have been linked to AH susceptibility in C. elegans and H. contortus. In order to predict the capacity of nematode Pgps to transport drugs, including ML and otherAH, we have developed an in silico drug docking model. We have used C. elegans Pgp-1 (Cel-Pgp-1) crystal structure and have showed a high affinity binding of several ligands that have been shown to be activators of its ATPase function. ML were also found to bind with high affinity to Cel-Pgp-1, on a specific binding site. This approach provides a valuable tool to predict drug-drug interactions and to rationally design new competitive inhibitors of nematode Pgps, in order to improve anthelmintic therapeutics. We then predicted a putative 3D structure of Hco-Pgp-13 based on the recently released crystal of Cel-Pgp-1, with which it presented a high homology. This allowed the study of the interaction of Hco-Pgp-13 with potential substrates, in particular ML. We found similar affinities for various drugs previously tested on Cel-Pgp-1, supporting the good homology of these two proteins. Together with in vitro ATPase assay experiments that confirmed the substrate status of actinomycin D, this indicates a possible multispecifc recognition capacity of this parasitic transporter. The determination of Hco-Pgp-13 localization using immunohistochemistry showed a wide tissue expression consistent with a critical role for Hco-Pgp-13 in endogenous and/or exogenous substrate transport. In conclusion, this work provides insights into the role of nematode Pgps in transporting AH drugs, both at the level of the model organism C. elegans and of the parasitic nematode H. contortus. This suggests a high homology of function conserved between ABC tranporters in these species. The localization of such protein on amphidial structures and its possible involvement in drug resistance and survival of H. contortus to exposure to AH compounds remain to be precised
Rehman, Saba [Verfasser], et Koepsell [Gutachter] Hermann. « Identification of accessible and closed substrate binding sites in the outward open cleft of rat Organic Cation Transporter 1 (rOCT1) / Saba Rehman ; Gutachter : Koepsell Hermann ». Würzburg : Universität Würzburg, 2018. http://d-nb.info/1170061710/34.
Texte intégralNimmagadda, Subbaiah Chary Verfasser], Thomas [Akademischer Betreuer] [Seufferlein, Sven-Erik [Akademischer Betreuer] Behrens et Michael [Akademischer Betreuer] Seckl. « Identification and characterization of Abelson Interactor 1, as a novel substrate of Protein Kinase D2 / Subbaiah Chary Nimmagadda. Betreuer : Thomas Seufferlein ; Sven-Erik Behrens ; Michael Seckl ». Halle, Saale : Universitäts- und Landesbibliothek Sachsen-Anhalt, 2013. http://d-nb.info/1037725433/34.
Texte intégralWasserman, Jacob Israel. « Progress toward the synthesis of structurally novel cytotoxic chlorinated diterpenes and identification of 3-isopropylmalate as an endogenous substrate of a methyl transferase in the yeast S. Cerevisiae ». Diss., Restricted to subscribing institutions, 2004. http://proquest.umi.com/pqdweb?did=765405381&sid=1&Fmt=2&clientId=1564&RQT=309&VName=PQD.
Texte intégralEggert, Erik. « Development of a cellular mechanistic assay for the SET and MYND domain containing methyltransferase SMYD2, identification and validation of a novel substrate, and functional characterization of its inhibition ». Doctoral thesis, Humboldt-Universität zu Berlin, 2017. http://dx.doi.org/10.18452/18145.
Texte intégralProtein methyltransferases are often misregulated in tumor cells and display a potential target for cancer therapy. The SET and MYND domain containing protein 2 (SMYD2) was described as a potential oncogene and overexpression correlated with a worse prognosis. Several substrates for SMYD2 had been described among them histone H3 and p53. However, the biology of SMYD2 is poorly understood. By developing a small molecule inhibitor of SMYD2 its therapeutic role could be better evaluated. Therefore, a cellular mechanistic assay was developed using a methylation specific antibody. With that assay BAY-598 was identified as a potent and selective cellular inhibitor of SMYD2. In the following a proteomic approach revealed hundreds of novel cellular lysine methylation sites in SMYD2 overexpression cells. Among these AHNAK protein was validated as a novel SMYD2 substrate, which was present in several cell lines as well as in muscle of mice. Finally, BAY-598 was used to test several hypothesized functions of SMYD2 in different cell line models. Taken together, the current work strongly supported the development of the probe inhibitor BAY-598 and the discovery of AHNAK as a novel SMYD2 methylation substrate. The relevance of SMYD2 and AHNAK methylation needs further investigation, which should be supported by BAY-598.
Alonso, Tarajano Manuel Alejandro. « Systematic identification and quantification of substrate specificity determinants in human protein kinases = Identificación y cuantificación sistemática de determinantes de la especificidad por sustrato en las proteínas quinasas de humano ». Doctoral thesis, Universitat de Barcelona, 2013. http://hdl.handle.net/10803/125027.
Texte intégralSundermann, Lena. « Identification and characterization of new Greatwall kinase substrates ». Thesis, Montpellier, 2018. http://www.theses.fr/2018MONTT016.
Texte intégralMitotic division is an essential phase of the cell cycle that ensures the correct repartition of the genetic content. Mitosis involves profound cellular reorganization that is mostly induced by massive protein phosphorylation. This phosphorylation is achieved thanks to the fine-tuning of the balance between kinases and phosphatases. At mitotic entry, protein phosphorylation is induced by the activation of the master kinase Cdk1-cyclin B and the inhibition of the phosphatase PP2A B55. Previous results from our and other laboratories recently discovered a new pathway essential to modulate PP2A-B55 during G2-M transition. This pathway includes the kinase Greatwall (GW) and its substrates Arpp19 and Ensa. At mitotic entry GW is activated and promotes the phosphorylation of Arpp19/Ensa converting them into potent inhibitors of PP2A B55. Surprisingly, no other substrates of GW have been identified so far. However, several pieces of data strongly suggest new roles of GW independently of Arpp19 and Ensa. The main aim of this work was the identification of new substrates of GW. To this end, I used several approaches including: (1) Biochemical fractionation of cell lysates or Xenopus egg extracts combined with in vitro phosphorylation with recombinant GW kinase, (2) SILAC/phosphoproteomics from cell lysates expressing different GW amounts, (3) Co-Immunoprecipitation, (4) BioID and (5) a candidate directed approach. Results from in vitro phosphorylation revealed the presence of two interesting phosphorylated bands that are currently being analysed. Both SILAC/phosphoproteomic and interactome approaches yielded the enrichment of proteins involved post-transcriptional regulation of gene expression and RNA related processes, a physiological function already described for this pathway in yeast. Finally, we directly investigated the putative phosphorylation by GW of three candidates known to be involved in the control of cell cycle. Although phosphorylated in vitro by GW, we could only identify the phosphorylation site in one of these three proteins. This protein, corresponding to a phosphatase inhibitor, appears to control mitotic exit through the modulation of mitotic protein dephosphorylation. A non-phosporylable mutant of this inhibitor promotes a perturbed mitotic exit with delayed dephosphorylation of mitotic substrates and impaired cyclin B degradation. I could attribute this defect to a perturbed association of the inhibitor with the phosphatase and consequently to an aberrant timing of phosphatase inhibition. Finally, I identified the GW phosphorylation site as a key factor controlling this association. In summary, I identified in this study a new substrate of GW controlling phosphatase activity essential for correct mitotic division
Eggert, Erik [Verfasser], Ana [Gutachter] Pombo, Bernard [Gutachter] Haendler et Ingo [Gutachter] Morano. « Development of a cellular mechanistic assay for the SET and MYND domain containing methyltransferase SMYD2, identification and validation of a novel substrate, and functional characterization of its inhibition / Erik Eggert ; Gutachter : Ana Pombo, Bernard Haendler, Ingo Morano ». Berlin : Humboldt-Universität zu Berlin, 2017. http://d-nb.info/1189330334/34.
Texte intégralJagdeo, Julienne. « Identification of novel picornavirus proteinase substrates using terminal amine isotopic labeling of substrates ». Thesis, University of British Columbia, 2017. http://hdl.handle.net/2429/61277.
Texte intégralMedicine, Faculty of
Biochemistry and Molecular Biology, Department of
Graduate
Birkin, Ria H. « The identification of novel protein kinase B substrates ». Thesis, University of Bristol, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.435741.
Texte intégralEhrhardt, Michael. « Identification of novel MARK3 substrates and upstream regulators ». Thesis, Bangor University, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.516120.
Texte intégralProbst, Brandon Linn. « Identification of substrates and pathways regulated by PAS kinase ». Access to abstract only ; dissertation is embargoed until after 12/20/2006, 2005. http://www4.utsouthwestern.edu/library/ETD/etdDetails.cfm?etdID=137.
Texte intégralKinstrie, Ross Stuart. « Identification of Drosophila DYRK family substrates and interacting proteins ». Thesis, University of Glasgow, 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.433084.
Texte intégralBerwick, Daniel. « The identification of novel substrates of protein kinase B ». Thesis, University of Bristol, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.274824.
Texte intégralSylvestre-Gonon, Elodie. « Caractérisation biochimique et structurale de quelques glutathion transférases de la classe Tau d'arabette (Arabidopsis thaliana) et de peuplier (Populus trichocarpa) ». Electronic Thesis or Diss., Université de Lorraine, 2020. http://www.theses.fr/2020LORR0253.
Texte intégralGlutathione transferases (GSTs) constitute a ubiquitous multigene superfamily of enzymes involved in xenobiotic detoxification and secondary metabolism. Canonical GSTs consist of an N-terminal thioredoxin domain and a α-helical C-terminal domain. In terrestrial plants, GSTs can be grouped in 14 classes but also according to the conserved residue found in their catalytic site either cysteine (Cys-GSTs) or serine (Ser-GSTs) GSTs. Ser-GSTs exhibit reduction of peroxides and/or glutathione (GSH) conjugation activities while Cys-GSTs rather exhibit deglutathionylation and dehydroascorbate reductase activities. Some of them also appear to have non-catalytic ligandin properties for the transport or storage of various molecules. The plant-specific Tau GST (GSTU) class is usually the most expanded one. The GSTUs are often over-expressed during biotic and abiotic stresses contributing notably to herbicide detoxification. However, the physiological role of most GSTUs is still poorly documented in planta. By combining phylogenetic, biochemical and structural approaches, this work led to the characterisation of nine GSTUs from Arabidopsis thaliana (AtGSTUs) and six GSTUs from Populus trichocarpa (PtGSTUs). Phylogenetic analysis of the Ser-GSTs present in photosynthetic organisms revealed that the expansion of GSTUs occurred concomitantly with the appearance of vasculature in plants, although some mosses and bryophytes possess GSTUs. Within an organism, GSTUs can be classified into distinct groups according to their catalytic motif. Enzymatic tests using recombinant proteins showed that almost all studied GSTUs exhibit GSH conjugation and peroxide reduction activities against different model substrates (CDNB, isothiocyanate derivatives, hydroperoxides). The three-dimensional structures of two GSTUs have been resolved and these adopt the classical canonical GST fold with some notable difference between them. The biochemical and structural analyses of these AtGSTUs and PtGSTUs further showed that some of them bind bacterial porphyrins while others bind polyphenolic compounds. Among the enzyme-ligand complexes identified, the structure of a bacalein-GSTU has been solved. The use of metabolites enriched samples extracted from A. thaliana and P. trichocarpa is the next step to decipher the role of GSTUs in planta
Shuaib, Ahmad. « Optimisation thermique de lasers à cavité vertical à base d’InP et reportés sur substrat silicium, pour des applications télécom ». Rennes, INSA, 2011. http://www.theses.fr/2011ISAR0014.
Texte intégralFor the last years, Vertical cavity surface emitting lasers (VCSELs) have become of large commercial importance since they are the dominant low cost, low power coherent sources in many applications such as short distance data communication. However, despite their great success, there remain opportunities for improvement, in particular in the fiber-to-the-home (FTTH) segment which needs 1550 nm long-wavelength VCSELs, based on the InP technology. Indeed, the FTTH infrastructure would develop only if the optical network becomes less costly, that is only if the laser component becomes more cheaper by replacing the edge-emitting lasers by VCSEL lasers. In particular, to be cost-effective, these devices must avoid complicating factors like cooling systems. Indeed, the high temperature generated in the active region is one of the limiting factors in achieving high power operation. This thesis work is focused on investigating the effects of thermal management on improving properties of optically-pumped VCSELs in order to achieve higher thermal performance devices. In particular, improved thermal performance has been obtained by using (a-SiH/a-SiNx)-based efficient dielectric Bragg mirror bonded on silicon substrate. Moreover, we found that a broad heat spreading in the intra-cavity InP layer is essential for obtaining low thermal resistance, we report on an optimisation of the intra-cavity InP thickness. We finally introduce a new design for the VCSEL device by using a bottom Bragg mirror patterned and embedded inside a metallic layer. Such device has been studied and implemented. Continuous wave (CW) single mode laser operation is demonstrated at room temperature (RT) with a photopumping experiment. The optimisations on the optically pumped VCSELs shown in this thesis work can be used in electrically pumped VCSEL
Yap, Jessica. « Identification of Plasmodium falciparum protein kinase substrates and interacting proteins ». Honors in the Major Thesis, University of Central Florida, 2012. http://digital.library.ucf.edu/cdm/ref/collection/ETH/id/644.
Texte intégralB.S.
Bachelors
Burnett School of Biomedical Sciences
Molecular and Microbiology
Floen, Miranda J. « Thioredoxin-1| Identification of redox substrates and response to hyperoxia ». Thesis, University of South Dakota, 2016. http://pqdtopen.proquest.com/#viewpdf?dispub=10132866.
Texte intégralBronchopulmonary dysplasia (BPD) is a serious respiratory complication for the preterm newborn characterized clinically by prolonged respiratory distress and histologically by alveolar simplification and decreased pulmonary vasculature. The development of BPD is well linked to oxidative stress suffered by the newborn as a result of a preterm fetal-neonatal transition, supplemental oxygen, infection, increased inflammation, and mechanical ventilation. Damage suffered by oxidative stress may be through direct mechanisms or through alteration of redox¬sensitive pathways involved in cell death, cell survival, differentiation, and proliferation. Redox¬sensitive modifications regulating protein function and redox-sensitive pathways have mainly been ascribed to oxidative modification of cysteine thiols. As their modification is critical for protein function, maintenance of the thiol redox status is crucial. Thioredoxin-1 (Trx1) functions in maintenance of thiol redox homeostasis, and its redox activity is intimately linked to antioxidant, cytoprotection, proliferation responses, and cytoprotection. While Trx1 targets of redox regulation have been identified, we hypothesize that additional protein may be redox regulated and that Trx1 target profiles may change during oxidative stress. Therefore a novel immunoprecipitation approach, identified as the substrate trap approach, was developed to identify Trx1 targets. The following demonstrates the use of the substrate trap approach for identification of Trx1 redox targets and further application of the approach to identify alterations in target profiles in response to oxidative stress. Use of nuclear targeted substrate trap was successfully employed to enrich from nuclear Trx1 targets. As a final component the characterization of the Trx1 system in mouse from late embryonic development through the first week of life animals were exposed to room air or hyperoxia (model of BPD). Characterization indicates impairment of the Trx1 system in response to hyperoxic injury. As Trx1 is known to regulate proliferation, cell death, survival, differentiation pathways, impairment of the Trx1 system during early neonatal development may potentiate hyperoxic injury and alterations in lung development. Better understanding of Trx1 interactions occur through the substrate trap in a physiological model of BPD will help elucidate redox-signaling pathways involved in BPD pathogenesis.
Loflin, Paul T. (Paul Tracey). « Identification of Endogenous Substrates for ADP-Ribosylation in Rat Liver ». Thesis, University of North Texas, 1992. https://digital.library.unt.edu/ark:/67531/metadc277847/.
Texte intégralAgban, Amégninou. « Synthèse de substrats chromogéniques et fluorogéniques pour l'identification des bactéries ». Université Joseph Fourier (Grenoble), 1989. http://www.theses.fr/1989GRE19003.
Texte intégralShao, Wei 1970. « Identification of caspase-1 and caspase-3 substrates and study on caspase-1 substrates in glycolytic pathway ». Thesis, McGill University, 2007. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=100248.
Texte intégralGrimaldi, Giovanna. « Identification and roles of cell substrates of intracellular mono-ADP-ribosylation ». Thesis, Open University, 2011. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.551612.
Texte intégralKondacs, Laszlo. « Novel substrates for the improved detection and identification of pathogenic bacteria ». Thesis, University of Sunderland, 2018. http://sure.sunderland.ac.uk/10222/.
Texte intégralLee, Caroline H. « IDENTIFICATION OF NOVEL SUBSTRATES OF LRRK2, A PARKINSON’S DISEASE ASSOCIATED KINASE ». Case Western Reserve University School of Graduate Studies / OhioLINK, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=case1390561222.
Texte intégral