Thèses sur le sujet « Sequential and selective labeling »
Créez une référence correcte selon les styles APA, MLA, Chicago, Harvard et plusieurs autres
Consultez les 31 meilleures thèses pour votre recherche sur le sujet « Sequential and selective labeling ».
À côté de chaque source dans la liste de références il y a un bouton « Ajouter à la bibliographie ». Cliquez sur ce bouton, et nous générerons automatiquement la référence bibliographique pour la source choisie selon votre style de citation préféré : APA, MLA, Harvard, Vancouver, Chicago, etc.
Vous pouvez aussi télécharger le texte intégral de la publication scolaire au format pdf et consulter son résumé en ligne lorsque ces informations sont inclues dans les métadonnées.
Parcourez les thèses sur diverses disciplines et organisez correctement votre bibliographie.
Park, Joonsuk. « Using Sequential Sampling Models to Detect Selective Infuences : Pitfalls and Recommendations ». The Ohio State University, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=osu157470864789277.
Texte intégralTener, Greg. « ATTACKS ON DIFFICULT INSTANCES OF GRAPH ISOMORPHISM : SEQUENTIAL AND PARALLEL ALGORITHMS ». Doctoral diss., University of Central Florida, 2009. http://digital.library.ucf.edu/cdm/ref/collection/ETD/id/2631.
Texte intégralPh.D.
School of Electrical Engineering and Computer Science
Engineering and Computer Science
Computer Science PhD
Tamura, Tomonori. « Endogenous protein imaging and analysis in living cells by selective chemical labeling methods ». 京都大学 (Kyoto University), 2013. http://hdl.handle.net/2433/174965.
Texte intégralBeller, Nicole C. « Selective Pulse Chase-SILAC Labeling of Three-Dimensional Multicellular Spheroids for Global Proteome Analysis ». The Ohio State University, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=osu1586292839111678.
Texte intégralMeakin, James A. « Velocity selective preparations in Magnetic Resonance Imaging ». Thesis, University of Oxford, 2014. http://ora.ox.ac.uk/objects/uuid:a4247c64-d113-42e6-beee-5795e78a4cdc.
Texte intégralFlake, Darl D. II. « Separation of Points and Interval Estimation in Mixed Dose-Response Curves with Selective Component Labeling ». DigitalCommons@USU, 2016. https://digitalcommons.usu.edu/etd/4697.
Texte intégralMulakhudair, Ali R. « Microbubble mediated sequential saccharification, inactivation, and aerobic fermentation with in situ selective product removal ». Thesis, University of Sheffield, 2017. http://etheses.whiterose.ac.uk/18942/.
Texte intégralYasueda, Yuuki. « A method for chemical proteomics based on the selective localization of labeling molecules in living systems ». 京都大学 (Kyoto University), 2016. http://hdl.handle.net/2433/215578.
Texte intégralOchtrop, Philipp. « Selective protein functionalisation via enzymatic phosphocholination ». Doctoral thesis, Umeå universitet, Kemiska institutionen, 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-140349.
Texte intégralProteiner utgör huvudbeståndsdelen av alla biomolekyler i en cell. Dessa är involverade i alla cellulära processer som bestämmer cellens egenskaper. För att förstå de cellulära processerna är det nödvändigt att förstå proteinernas funktion på molekylär nivå. Att studera proteiner i deras naturliga omgivning, det vill säga inuti en cell eller i ett cellextrakt, är en stor utmaning i dagens livsvetenskaper. Eftersom proteiner är kemiskt lika varandra så är det svårt att skilja ett från tusentals andra. Att specifikt märka proteiner för att skilja ut dem från bakgrunden har blivit ett viktigt arbetssätt i modern biokemi och cellbiologi. Avhandlingen beskriver utvecklandet av en ny metod för reversibel och kovalent enzymatisk märkning baserat på fosfokolinering/defosfokolinering av en kort aminosyrasekvens i intakta proteiner. En syntesmetod för att framställa onaturliga CDP-kolinderivat har etablerats vilket tillåter oss att framställa CDP-kolin som bär en funktionalitet, vilket kan vara ett färgämne eller en affinitetstagg. Dessa onaturliga CDP-kolinderivat accepteras som co-substrat av enzymet AnkX från Legionella pneumophila vilket transfererar den funktionaliserade delen av CDP-kolinderivatet till en kort aminosyrasekvens baserad på AnkX’s naturliga substrat vid infektion, det lilla GTPaset Rab1. Under avhandlingsarbetets gång identifierades den kortaste aminosyrasekvensen som känns igen av AnkX, endast de åtta aminosyrorna TITSSYYR är nödvändiga för igenkänning av AnkX. Dessa åtta aminosyror kan genetiskt infogas i början, slutet eller mitt i ett protein för igenkänning och funktionalisering via AnkX och våra syntetiska CDP-kolinderivat. Vid Legionellainfektion i eukaryota celler klyvs fosfokolineringen efter en viss tid, eftersom Legionella pneumophila producerar ett fosfodiesteras, Lem3, som tar bort de fosfokolineringar som AnkX har installerat när de inte längre behövs. Vi har använt Lem3 för att ta bort märkning i sekvensen TITSS(PC)YYR, vilket gör vår strategi helt reversibel. Vi har kunnat demonstrera att AnkX-Lem3 systemet accepterar ett brett spektrum av CDP-kolinderivat, vilket gör metoden till den första av sitt slag, eftersom den är fullt reversibel. Vi har vidare undersökt vilka proteiner AnkX reagerar med inuti celler, vi använde oss av ett CDP-kolinderivat funktionaliserat med biotin, vilket har tillåtit oss att fiska ut alla de proteiner som fosfokolineras av AnkX. Förutom de små GTPaserna i Rab-familjen så identifierade vi även IMPDH2, ett enzym som reglerar det hastighetsbestämmande steget i syntesen av guanosin-nukleotider. Detta är mycket intressant, eftersom det leder till frågan ifall Legionella pneumophila manipulerar sin värdcell genom att förändra mängden GTP i förhållande till ATP.
Bock, Paul B. « The sequential self-selection auction mechanism for selective reenlistment bonuses potential cost savings to the U.S. Marine Corps ». Thesis, Monterey, Calif. : Naval Postgraduate School, 2007. http://bosun.nps.edu/uhtbin/hyperion-image.exe/07Jun%5FBock.pdf.
Texte intégralThesis Advisor(s): William R. Gates, Peter J. Coughlan. "June 2007." Includes bibliographical references (p. 67-68). Also available in print.
Grünewald, Maria. « Design and analysis of response selective samples in observational studies ». Doctoral thesis, Stockholms universitet, Matematiska institutionen, 2011. http://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-49328.
Texte intégralAt the time of doctoral defense, the following paper was unpublished and had a status as follows: Paper 1: Submitted.
Avdiyenko, Liliya. « Adaptive sequential feature selection in visual perception and pattern recognition ». Doctoral thesis, Universitätsbibliothek Leipzig, 2014. http://nbn-resolving.de/urn:nbn:de:bsz:15-qucosa-153417.
Texte intégralErdmann, Ines [Verfasser], et Daniela C. [Akademischer Betreuer] Dieterich. « Deciphering proteome dynamics using cell-type selective metabolic protein labeling in the fruit fly Drosophila melanogaster / Ines Erdmann ; Betreuer : Daniela C. Dieterich ». Magdeburg : Universitätsbibliothek, 2016. http://d-nb.info/1121079857/34.
Texte intégralErdmann, Ines Verfasser], et Daniela C. [Akademischer Betreuer] [Dieterich. « Deciphering proteome dynamics using cell-type selective metabolic protein labeling in the fruit fly Drosophila melanogaster / Ines Erdmann ; Betreuer : Daniela C. Dieterich ». Magdeburg : Universitätsbibliothek, 2016. http://nbn-resolving.de/urn:nbn:de:gbv:ma9:1-8483.
Texte intégralSadoine, Mayuri Verfasser], Jörg Ludwig [Akademischer Betreuer] Fitter et Ulrich [Akademischer Betreuer] [Schwaneberg. « Selective dual-labeling of cell-free synthesized proteins for single-molecule FRET studies : a case study of human calmodulin / Mayuri Sadoine ; Jörg Ludwig Fitter, Ulrich Schwaneberg ». Aachen : Universitätsbibliothek der RWTH Aachen, 2018. http://d-nb.info/1181335191/34.
Texte intégralSadoine, Mayuri [Verfasser], Jörg Ludwig Akademischer Betreuer] Fitter et Ulrich [Akademischer Betreuer] [Schwaneberg. « Selective dual-labeling of cell-free synthesized proteins for single-molecule FRET studies : a case study of human calmodulin / Mayuri Sadoine ; Jörg Ludwig Fitter, Ulrich Schwaneberg ». Aachen : Universitätsbibliothek der RWTH Aachen, 2018. http://d-nb.info/1181335191/34.
Texte intégralCutolo, Giuliano. « Etude du système Myrosinase-Glucosinolate comme outil de bioconjugaison ». Thesis, Orléans, 2018. http://www.theses.fr/2018ORLE2050.
Texte intégralSince many decades, Isothiocyanate (ITCs) reagents are widely used in bioconjugation approaches to create a stable bond onto the lysin and cysteine residues of proteins and peptides. Thanks to this click reaction it is possible to achieve a selective ligation or a high functionalization of proteins. On the others hand, isothiocyanates are not easy to prepare, to isolate, to store and most of the time insoluble in water.The aim of this work is to explore the myrosinase-glucosinolate (MG) enzymatic tandem as a ligation system, in order to release ITCs in-situ. The MG tandem is a well-known mechanism of defense in plants of the order Brassicales. In this biochemical system, myrosinase acts as a thioglucosidase, hydrolyzing glucosinolates (GLs) to liberate transient species that spontaneously form ITCs. The advantage of this enzymatic tandem is to generate ITCs from a stable non-toxic GLs precursor, soluble in water, using mild conditions.In order to develop this enzymatic tool, two kind of unnatural GLs were synthetized. Due to the important role of the mannoside-lectins interaction in the bacterial adhesion mechanism, at first, we designed a small library of GL bearing mannosides, in order to target and study the bacterial lectin FimH. The second kind of GL possess a chemical function allowing to study orthogonal reactions. After, the ability of the myrosinase to hydrolyze those unnatural GLs was investigated, as well their chemical reactivity.Then, the performances of the MG system was studied in different approaches of bioconjugation, such as: the synthesis of neoglycoproteins (NGPs), the site selective labeling of a lectin and the functionalization of gold nanoparticles. The feasibility of these strategies confirmed that the MG system can be used as an enzymatic tool in some bioconjugation approaches
MAZZOLENI, ELISA. « Exploration of new techniques for purification and chemo-selective conjugation of bioreagents for immunodiagnostic applications ». Doctoral thesis, Università degli Studi di Milano-Bicocca, 2015. http://hdl.handle.net/10281/68468.
Texte intégralAntigen and antibody are the two key reagents for an immunodiagnostic assay. Investigation of new techniques and improvement of processes such as purification and site-specific labeling of antigen and antibody molecules can promote the development of new more powerful bioreagents able of improving the performance of immunodiagnostic assays. The first part of this thesis aimed to explore innovative biotechnology techniques in antigen production for the improvement of immunoassays that allow the detection of antibodies directed against the Epstein-Barr virus. EBV virus is the causative agent of infectious mononucleosis and it is considered to be associated with a still increasing number of tumors; for this reason it is important to develop diagnostic assays for EBV detection with high specificity and sensitivity. The viral capsid protein VCA p18 is one of the most important antigens for the diagnosis of EBV. The current Diasorin LIAISON EBV VCA IgM and IgG assays rely on a single antigen, consisting in a synthetic peptide corresponding to the immunodominant C-terminal portion of the p18 protein, which is immobilized on solid phase (indirect format). The several methods explored in this thesis have allowed to obtain different variants of the p18 antigen with the aim to improve the performance of DiaSorin LIAISON EBV VCA IgM and IgG assays at different levels: 1_production of p18 antigen; 2_immobilization of p18 antigen on solid phase; 3_immunoassay format. 1_ The length of the immunodominant C-terminal portion of the p18 protein (57aa) appears to be considerable for the synthetic route but, at the same time, too small to be effectively produced in a recombinant fashion. To overcome this problem, we explored the Elastin Like Polypeptides (ELP)-Intein system, a method based on the use of a self-cleavable protein (the intein) and a temperature responsive tag (ELP). This technique has proved to be an excellent system for the preparation of the p18 peptide. 2_The development of different variants of p18 antigen has enabled to explore different techniques for the immobilization of the same antigen on solid phase: direct covalent coating, through streptavidin-biotin complex and through an innovative procedure based on the use of leucine zipper (or “velcro”) peptides. The immobilization of the p18 peptide on solid phase through these different methods occurred successfully and the immunochemical activity of the antigen, immobilized with these innovative techniques, is comparable or better than that of the synthetic peptide used in the current immunoassays. 3_Despite the DiaSorin LIAISON EBV VCA IgM immonoassay has a good analytical performance, in order to obtain an increase of specificity, a new assay format was explored. Unfortunately, the results indicate that this different type of format reaches a lower level of specificity than that of current assay. The second part of this thesis aimed to explore an innovative method for the site-specific labeling of antibodies. One the most promising approach is based on the generation of free thiol groups by selective partial reduction of the interchain disulfide bridges present at the level of the “hinge region” and their reaction to labels carrying sulfhydryl-reactive chemical groups. This technology was used for the biotinylation of two different antibodies currently used in immunoassays for the HIV p24 viral protein and for FGF23 antigen detection. The results suggest that the site-specific biotinylation compared to the random traditional biotinylation promotes a great improvement of antibodies immunochemical activity with a consequent optimization of immunodiagnostic assays performance.
Baumeister, Paul [Verfasser], Armin [Akademischer Betreuer] Buschauer et Sigurd [Akademischer Betreuer] Elz. « Molecular tools for G-protein coupled receptors : Synthesis, pharmacological characterization and [3H]-labeling of subtype-selective ligands for histamine H4 and NPY Y2 receptors / Paul Baumeister. Betreuer : Armin Buschauer ; Sigurd Elz ». Regensburg : Universitätsbibliothek Regensburg, 2014. http://d-nb.info/1054802874/34.
Texte intégralGhabel, Vahid Reza. « Design and Development of a Novel Remediation Process for Metal Production From Berkeley Pit Acid Mine Drainage ». University of Cincinnati / OhioLINK, 2007. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1194966560.
Texte intégralPegoli, Andrea [Verfasser], et Armin [Akademischer Betreuer] Buschauer. « Dibenzodiazepinone-type mAChR ligands : radio- and fluorescence labeling enable unveiling of dualsteric M₂R binding and conjugation to short peptides as an avenue to highly selective M₂R ligands / Andrea Pegoli ; Betreuer : Armin Buschauer ». Regensburg : Universitätsbibliothek Regensburg, 2017. http://d-nb.info/1210701731/34.
Texte intégralCheung, Anthony Hing-lam. « Design and implementation of an Arabic optical character recognition system ». Thesis, Queensland University of Technology, 1998. https://eprints.qut.edu.au/36073/1/36073_Cheung_1998.pdf.
Texte intégralSECCI, ERICA. « New strategy of protein expression in mammalian cells for in-cell NMR ». Doctoral thesis, 2015. http://hdl.handle.net/2158/1015496.
Texte intégralHuang, Chih Kai, et 黃致凱. « Named Entity Recognition in Difangzhi Using Sequential Labeling Techniques ». Thesis, 2016. http://ndltd.ncl.edu.tw/handle/k77974.
Texte intégral國立政治大學
資訊科學學系
104
Difangzhi is the local gazetteers compiled by local government of China. Its content is plenty and extensive. It’s including many undetected information, like biographical information, geographical information, and officer record information and so on. Because of the difference between Difangzhi corpus and modern Chinese language, we should not use current natural language processing tools directly. In order to extract biographical information, we construct our model to recognize the named entity and use the noun list to assist our annotation method in Difangzhi corpus. In this study, we use supervised learning to construct our model. At first, we need to generate our training data. According to the personal information list with manual verification and noun lists, we have reliable information to annotate words in Difangzhi corpus. However, they still have some noise in those lists. As a result, we must do the preprocessing to those lists for cleaning. After, the ambiguity problem will happen when we trying to annotate our corpus. Here we provide three methods to annotate our corpus with disambiguation. Using the annotated corpus to generate training data and built the condition random fields models. In our experiment, we use our models generated by three different annotate methods to predict the character label in testing Difangzhi corpus. According to the labeled result, we extract the person name and address name to evaluate. The result shows the precision of person name recognition is over 80%, and precision of address name recognition is about 86%. Because of the training corpus and test corpus is quite similar, the performances of our model is pretty well. Therefore, we use labeled result to find correlation of person name and address name. Using a simple way to connect person name and address name and sampling the result to evaluate. The sample result shows we could connect person name and address name correctly in some specific grammars. In order to analyze more deeply, we attempt to split clauses in Difangzhi corpus. Use finite state machine model to recognize the beginning of clauses. Although the result shows we could find some beginning of clauses, but our method still lose many beginning of clauses. In the future work, we attempt to add more information to annotate Difangzhi corpus and modify our disambiguated methods to make the recognition result better. In order to get more information about the person in the corpus, we will try to split paragraphs or sentences more precisely. Besides, we also try to analyze grammar in the corpus. Finding useful pattern to connect person name and other entities, like address name, officer name and so on. Generating the information about people appears in the corpus automatically.
Yen, Po-Jen, et 顏伯任. « Sequential asymmetric oganocatalytic anti-selective Michael reaction-Reduction-Lactonization-Pauson-Khand reaction ». Thesis, 2012. http://ndltd.ncl.edu.tw/handle/02527947231986221379.
Texte intégral國立中正大學
化學暨生物化學研究所
100
A steroselectivity synthesis of fully subsititude decahydro-1H- pentaleno [2,1-c]pyran derivatives have been achieved with alkylidene malonates and aldehydes. By a sequential oganocatalyzed anti-selective Michael reaction-reduction-lactonization-Pauson-Khand reaction, varios additives effected different results of steroselectivity.
Choi, Christopher Hyun-Bae. « Rapid sequential corneal topography evaluation of selective suture removal in the management of post-keratoplasty astigmatism ». Thesis, 2019. https://hdl.handle.net/2144/36266.
Texte intégralKitowski, Annabel Katharina. « Bio-orthogonal site-selective labelling of carbohydrates and proteins ». Doctoral thesis, 2019. http://hdl.handle.net/10451/44169.
Texte intégralYuan, Chin, et 張智媛. « Nickel Hexacyanoferrate Multilayers on Organically Modified Sol-Gel Silica Prepared upon Multiple Sequential Adsorption for Selective Sorption and Sensing of Cesium ». Thesis, 2005. http://ndltd.ncl.edu.tw/handle/91915283476891972853.
Texte intégral國立中正大學
化學所
94
Radioactive cesium contamination of water is of serious social and environmental concern since it is a significant fraction of the radioactivity of the liquid waste. Nickel hexacyanoferrate (NiHCF) has been widely studied for separation and electrochemical sensing of Cs+ owing to their strong selectivity for Cs+ over other alkali cations. In this work, a novel material, multilayers of NiHCF immobilized within or on a porous propyl-ethylene diamine triacetate functionalized silica matrix, has been synthesized and characterized. Its uses as a novel cesium sorbent and cesium sensing material have been studied. As a cesium sorbent, this material was evaluated for selective removal of cesium ions in the presence of competing metal ions from water. Loading capacity of at least 1.69 mmole per g of sorbent material has been achieved. As a cesium sensing material, multilayers of NiHCF were coated on glassy carbon electrodes by multiple sequential adsorption for potentiometric sensing of cesium ions. A Nernst response with a slope of 31 mV/decade was obtained between 10-6 M and 1 M. The material was characterized through infrared spectroscopy, X-ray powder diffraction, cyclic voltammetry, X-ray energy dispersive spectroscopy and the Ni and Fe precursors were modeled by the PM3TM semiempirical method.
Chen, Chiu-Yueh, et 陳秋月. « Use of Rhodostomin to Study the Integrin Recognition Sequences and to Establish a Method for Preparing Amino-Acid-Type Selective Isotope Labeling of Proteins ». Thesis, 2005. http://ndltd.ncl.edu.tw/handle/09874708733395048904.
Texte intégral國立成功大學
基礎醫學研究所
94
Integrins are alpha/beta�nheterodimers on cellular membrane and involved in cell adhesion, mobility, and signal transduction. Many physiological and non-physiological integrin ligands utilize RGD, LDV, or a related sequence as a key structural component of their integrin-binding site. Rhodostomin (Rho) is a potent integrin antagonist, which consists of 68 amino acids including six disulfide bonds and a PRGDMP sequence at the positions of 48-53. Rho competes against fibrinogen in the binding of integrin �娗lphaIIbbeta3 on platelet and results in inhibiting platelet aggregation. Many studies have also demonstrated that the amino acid residues flanking the RGD sequence of integrin ligands modulate their specificity of interaction with integrin alphaIIbbeta3,alphaVbeta3, and alpha5beta1 complexes. In order to identify the amino acid residues required for selective recognition of integrins alphaIIbbeta3, alphaV�涀eta3, and�nalpha5beta1, we mutated the residues in the RGD loop or C-terminal region of Rho, expressed Rho mutants in P. pastoris, and used the platelet aggregation and cell adhesion assays for functional studies. We found that Rho mutants containing the sequences AKGDWN, as well as RLD and RGDD can selectively inhibit integrins alphaIIbbeta3 and alphaVbeta3, respectively. In addition, the mutants containing the sequences ARGDGW and ARGDXX have better selectivity or activity in inhibiting integrin alpha5beta1. 3D structures of integrin-specific Rho mutants were determined by NMR spectroscopy. Our results showed that the orientations of R49 and D51 residues and dynamic properties of the RGD motif in Rho play important roles in integrin recognition. To develop the protocol to produce amino-acid-type selective (AATS) isotope labeling of protein expressed in P. pastoris, Rho was used to optimize the culture condition. The optimized protocol was successfully applied to produce AATS isotope-labeled Rho and other protein, dendroaspin. The labeling of [alpha-15N]-Cys, -Leu, -Lys, and -Met amino acids has an incorporation rate greater than 50%. In addition, the culture condition was successfully applied to label dendroaspin, a four disulfide-bonded protein expressed in P. pastoris. This is the first study to present a protocol for AATS isotope labeling of protein expressed in P. pastoris. This study will serve as the basis for exploring structure-activity relationships of the integrin/disintegrin complexes as well as rational drug design on integrins.
Abé, Christoph. « Conformational Changes Of Vinculin Tail Upon F-Actin And Phospholipid Binding Studied By EPR Spectroscopy ». Doctoral thesis, 2010. https://repositorium.ub.uni-osnabrueck.de/handle/urn:nbn:de:gbv:700-201006296359.
Texte intégralMobasher, Amin. « Applications of Lattice Codes in Communication Systems ». Thesis, 2007. http://hdl.handle.net/10012/3452.
Texte intégral