Littérature scientifique sur le sujet « RP4 plasmide conjugatif »
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Articles de revues sur le sujet "RP4 plasmide conjugatif"
Samuels, A. Lacey, Erich Lanka et Julian E. Davies. « Conjugative Junctions in RP4-Mediated Mating ofEscherichia coli ». Journal of Bacteriology 182, no 10 (15 mai 2000) : 2709–15. http://dx.doi.org/10.1128/jb.182.10.2709-2715.2000.
Texte intégralCairns, Johannes, Matti Jalasvuori, Ville Ojala, Michael Brockhurst et Teppo Hiltunen. « Conjugation is necessary for a bacterial plasmid to survive under protozoan predation ». Biology Letters 12, no 2 (février 2016) : 20150953. http://dx.doi.org/10.1098/rsbl.2015.0953.
Texte intégralBackert, Steffen, Terry Kwok et Wolfgang König. « Conjugative plasmid DNA transfer in Helicobacter pylori mediated by chromosomally encoded relaxase and TraG-like proteins ». Microbiology 151, no 11 (1 novembre 2005) : 3493–503. http://dx.doi.org/10.1099/mic.0.28250-0.
Texte intégralBates, Steven, Annette M. Cashmore et Brian M. Wilkins. « IncP Plasmids Are Unusually Effective in Mediating Conjugation of Escherichia coli and Saccharomyces cerevisiae : Involvement of the Tra2 Mating System ». Journal of Bacteriology 180, no 24 (15 décembre 1998) : 6538–43. http://dx.doi.org/10.1128/jb.180.24.6538-6543.1998.
Texte intégralHamilton, Claire M., Hyewon Lee, Pei-Li Li, David M. Cook, Kevin R. Piper, Susanne Beck von Bodman, Erich Lanka, Walt Ream et Stephen K. Farrand. « TraG from RP4 and TraG and VirD4 from Ti Plasmids Confer Relaxosome Specificity to the Conjugal Transfer System of pTiC58 ». Journal of Bacteriology 182, no 6 (15 mars 2000) : 1541–48. http://dx.doi.org/10.1128/jb.182.6.1541-1548.2000.
Texte intégralDimitriu, Tatiana, Andrew C. Matthews et Angus Buckling. « Increased copy number couples the evolution of plasmid horizontal transmission and plasmid-encoded antibiotic resistance ». Proceedings of the National Academy of Sciences 118, no 31 (29 juillet 2021) : e2107818118. http://dx.doi.org/10.1073/pnas.2107818118.
Texte intégralJalasvuori, Matti, Ville-Petri Friman, Anne Nieminen, Jaana K. H. Bamford et Angus Buckling. « Bacteriophage selection against a plasmid-encoded sex apparatus leads to the loss of antibiotic-resistance plasmids ». Biology Letters 7, no 6 (juin 2011) : 902–5. http://dx.doi.org/10.1098/rsbl.2011.0384.
Texte intégralRabel, Christian, A. Marika Grahn, Rudi Lurz et Erich Lanka. « The VirB4 Family of Proposed Traffic Nucleoside Triphosphatases : Common Motifs in Plasmid RP4 TrbE Are Essential for Conjugation and Phage Adsorption ». Journal of Bacteriology 185, no 3 (1 février 2003) : 1045–58. http://dx.doi.org/10.1128/jb.185.3.1045-1058.2003.
Texte intégralLian, Zheng Jie, Minh-Duy Phan, Steven J. Hancock, Nguyen Thi Khanh Nhu, David L. Paterson et Mark A. Schembri. « Genetic basis of I-complex plasmid stability and conjugation ». PLOS Genetics 19, no 6 (22 juin 2023) : e1010773. http://dx.doi.org/10.1371/journal.pgen.1010773.
Texte intégralPicardeau, Mathieu. « Conjugative Transfer between Escherichia coli and Leptospira spp. as a New Genetic Tool ». Applied and Environmental Microbiology 74, no 1 (9 novembre 2007) : 319–22. http://dx.doi.org/10.1128/aem.02172-07.
Texte intégralThèses sur le sujet "RP4 plasmide conjugatif"
Djermoun, Sarah. « Le plasmide RP4 : de son utilisation comme outil antibactérien à l’étude de sa dynamique de transfert au sein de biofilm bactérien ». Electronic Thesis or Diss., Lyon 1, 2023. http://www.theses.fr/2023LYO10080.
Texte intégralThe study of conjugation dynamics of conjugative plasmids in Gram-negative bacteria is the central research theme of our laboratory and around which my thesis project was built. The aim of my research was to provide real knowledge on the extent and impact of conjugation in bacterial communities. The biofilm is widely considered by the scientific community as a hotspot for gene transfer mainly because of the favorable cell contacts that exist in its structure. However, the only studies that have attempted to demonstrate experimentally that biofilms increase gene transfer by conjugation do not provide clear data on the dynamics of these transfers that take place in the biofilm and how the biofilm impacts these transfers. The approach we used to study the dynamics of conjugation in biofilm is based on a collaborative project between our laboratory and that of Dr. Knut Drescher, based at the Biozentrum in Basel, Switzerland. This collaboration allowed us to deploy innovative fluorescence microscopy techniques developed by our two laboratories and never used before in the context of the study of conjugation in biofilm.We focused on the RP4 plasmid which is an IncP conjugative plasmid. Found within many natural environments, it has been the primary plasmid model for studies that have focused on conjugation in the biofilm, and has been widely exploited as a genetic tool by the scientific community. Despite the fact that it has been widely used, the transfer mechanisms of the RP4 plasmid are very poorly described. The RP4 plasmid has thus proven to be a very relevant model for studying conjugation that we have used both in a biotechnological aspect to broaden the host spectrum of antibacterial TAPs systems and in a fundamental aspect to study its conjugation dynamics, both within a 2D cultured E. coli population and within a 3D biofilm structured E. coli population.During my thesis work, I therefore exploited the RP4 plasmid to carry antibacterial CRISPR systems in various phylogenetically distant bacterial species. I provided the first real-time images of the RP4 plasmid transfer in 2D and very interesting new data on the timing of DNA double-strand conversion in the recipient. Finally, a totally innovative approach allowed to study the conjugation dynamics of the RP4 plasmid in the biofilm. These results finally constitute the first study that really describes how conjugation takes place in the biofilm and that goes beyond in terms of understanding this dynamic thanks to the 2D approach that we had set up. We demonstrate that biofilm is not a hotspot for the transfer of the RP4 plasmid and that the factors of the EPS matrix that compose its structure do not prevent the dissemination of the plasmid. Rather, it is the stage of biofilm development that makes it possible for the donors to attach to the surface contact areas near the recipient cells
Chapitres de livres sur le sujet "RP4 plasmide conjugatif"
Stabb, Eric V., et Edward G. Ruby. « RP4-based plasmids for conjugation between Escherichia coli and members of the vibrionaceae ». Dans Bacterial Pathogenesis Part C : Identification, Regulation, and Function of Virulence Factors, 413–26. Elsevier, 2002. http://dx.doi.org/10.1016/s0076-6879(02)58106-4.
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