Articles de revues : « Rosmarinus officinali »

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Li, Guopeng, Claudio Cervelli, Barbara Ruffoni, Alona Shachter et Nativ Dudai. « Volatile diversity in wild populations of rosemary ( Rosmarinus officinali s L.) from the Tyrrhenian Sea vicinity cultivated under homogeneous environmental conditions ». Industrial Crops and Products 84 (juin 2016) : 381–90. http://dx.doi.org/10.1016/j.indcrop.2016.02.029.

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Zhang, Ying, Xiaoqiang Chen, Lei Yang, Yuangang Zu et Qi Lu. « Effects of rosmarinic acid on liver and kidney antioxidant enzymes, lipid peroxidation and tissue ultrastructure in aging mice ». Food & ; Function 6, n^o 3 (2015) : 927–31. http://dx.doi.org/10.1039/c4fo01051e.

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Тохсырова (Tokhsirova), Залина (Zalina) Маирбековна (Mairbekovna), Иван (Ivan) Викторович (Viktorovich) Попов (Popov) et Ольга (Ol'ga) Ивановна (Ivanovna) Попова (Popova). « THE STUDY OF PHENOLIC COMPOUNDS THE LEAVES AND SHOOTS OF ROSEMARY (ROSMARINUS OFFICI-NALIS L.), INTRODUCED IN BOTANICAL GARDEN OF PYATIGORSK MEDICAL-PHARMACEUTICAL INSTITUTE ». chemistry of plant raw material, n^o 3 (27 mars 2018) : 199–207. http://dx.doi.org/10.14258/jcprm.2018033733.

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The object of the study was the leaves and shoots of rosemary medicinal (Rosmarinus officinalis L.), introduced for 9 years in the conditions of Caucasian Mineral Waters (North Caucasus) in the Botanical garden of the Pyatigorsk medical and pharmaceutical Institute in the open ground with shelter for the winter. Preliminary identification of phenolic compounds in the extracts from leaves and shoots of rosemary were carried out by thin-layer chromatography (TLC), and found gallic acid, rosmarinic acid, quercetin and apigenin.The method of high-performance liquid chromatography (HPLC) at wavelengths of 254 and 330 nm in leaves and shoots rosemary drug found 10 compounds: flavonoids: catechin, epicatechin, quercetin, apigenin; phenolcarboxylic acids: caffeic, gallic, chlorogenic, ferulic, rosmarinic as well as ascorbic acid. The content of rosmarinic acid in terms of dry raw materials in rosemary leaves was 0.181–0.184%; in shoots 0.062–0.064%.The area of the Northern Caucasus can be considered as a promising area for cultivation of medicinal plants – rosemary medicinal (Rosmarinus officinalis) – for the production of medicinal plant raw materials with a high content of biologically active substances.

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Chaul, Luiza Toubas, Virgínia Farias Alves, Stone de Sá, Leandra de Almeida Ribeiro Oliveira, Tatiana de Sousa Fiuza, Ieda Maria Sapateiro Torres, Edemilson Cardoso da Conceição et José Realino de Paula. « Antimicrobial Activity an Physicochemical Characterization of Extracts and Fractions of Rosmarinus officinalis and Origanum vulgare ». Fronteiras : Journal of Social, Technological and Environmental Science 11, n^o 1 (29 avril 2022) : 8–30. http://dx.doi.org/10.21664/2238-8869.2022v11i1.p8-30.

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Rosmarinus officinalis L. (Lamiaceae) is a shrubby woody with bittersweet taste, used in chips, broths, desserts, cookies, jams, fruit salads, and marmalades. Origanum vulgare L. (Lamiaceae) is a small plant used in meats, salads, soups, rice, potatoes and boiled eggs, as well as for perfuming and flavoring oils. Both have medicinal and aromatic properties. The aims of this study were: characterize, standardize herbal drugs, crude ethanol extracts and concentrated extracts of R. officinalis and O. vulgare, determine the polyphenols, tannins, flavonoids content; quantify the rosmarinic acid; evaluate the antimicrobial activity of rosmarinic acid, extracts, fractions of R. officinalis and O. vulgare against foodborne and clinical strains. The antimicrobial activity was evaluated by the broth microdilution method. The hexane and dichloromethane fractions of R. officinalis and O. vulgare and rosmarinic acid exhibited good inhibitory activity against some Gram-positive bacteria and food isolates. Ethyl acetate fraction of R. officinalis, concentrated extract, and fractions of O. vulgare and rosmarinic acid presented good inhibitory activity against Cryptococcus. These fractions have promising potential in the control of food pathogens and they can be used in the future as an alternative natural preservative in the industry.

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Elansary, Hosam O., Agnieszka Szopa, Paweł Kubica, Halina Ekiert, Diaa O. El-Ansary, Fahed A. Al-Mana et Eman A. Mahmoud. « Saudi Rosmarinus officinalis and Ocimum basilicum L. Polyphenols and Biological Activities ». Processes 8, n^o 4 (10 avril 2020) : 446. http://dx.doi.org/10.3390/pr8040446.

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Investigating the polyphenolic profile of natural Rosmarinus officinalis and Ocimum basilicum populations may reveal essential compounds that have biological activities. Natural populations of R. officinalis and O. basilicum in Northern Riyadh were investigated by HPLC-DAD analyses. Several polyphenols, including rosmarinic acid, gentisic acid, 3,4-dihydroxyphenylacetic acid, rutoside, and others, out of 38 screened were confirmed. Rosmarinic acid was the major polyphenol in both of R. officinalis and O. basilicum. R. officinalis methanolic leaf extracts contained other phenols such as gentisic acid while O. basilicum contained also 3,4-dihydroxyphenylacetic acid and rutoside as well as others. R. officinalis showed higher antioxidant activities than O. basilicum using 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP), and β-carotene bleaching assays. These higher activities are associated with a higher composition of rosmarinic acid in leaf extracts. The antioxidant activities of O. basilicum were attributed to identified phenols of rosmarinic acid, 3,4-dihydroxyphenylacetic acid, and rutoside. There were antiproliferative and cytotoxic activities of leaf extracts, as well as identified polyphenols, against several cancer cells. These activities were attributed to the accumulation of necrotic and apoptotic cells in treated cancer cells with leaf extracts as well as identified polyphenols. The antibacterial and antifungal activities of leaf extracts were mainly attributed to 3,4-dihydroxyphenylacetic acid and rutoside in O. basilicum and rosmarinic acid and caffeic acid in R. officinalis. This study proved that R. officinalis and O. basilicum natural populations might be considered as promising sources of natural polyphenols with biological activities.

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Kompelly, Akshay, Swathi Kompelly, Bakshi Vasudha et Boggula Narender. « Rosmarinus officinalis L. : an update review of its phytochemistry and biological activity ». Journal of Drug Delivery and Therapeutics 9, n^o 1 (15 janvier 2019) : 323–30. http://dx.doi.org/10.22270/jddt.v9i1.2218.

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Herbal medicine is one of the oldest valuable bestowals that were given to mankind. Many plants and herbs hold their prestigious position in the field of medicine. The worldwide interest in the use of medicinal plants has been growing, and its beneficial effects being rediscovered for the development of new drugs. Based on their vast ethnopharmacological applications, which inspired current research in drug discovery, natural products can provide new and important leads against various pharmacological targets. This work pioneers an extensive and an updated literature review on the current state of research on Rosmarinus officinalis L., elucidating which compounds and biological activities are the most relevant. According to these references, there has been an increasing interest in the therapeutic properties of this plant, regarding carnosic acid, carnosol, rosmarinic acid and the essential oil. The present manuscript provides an updated review upon the most reported activities on R. officinalis and its active constituents. This knowledge about the medicinal plants usage can also be extended to other fields like field of pharmacology. In view of the nature of the plant, more research work can be done on humans so that a drug with multifarious effects will be available in the future market. Keywords: Rosmarinus officinalis, phytochemicals, carnosic acid, rosmarinic acid, neuroprotective activity, anti-diabetic activity.

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Sik, Beatrix, Erika Hanczné Lakatos, Viktória Kapcsándi, Rita Székelyhidi et Zsolt Ajtony. « Investigation of the long-term stability of various tinctures belonging to the lamiaceae family by HPLC and spectrophotometry method ». Chemical Papers 75, n^o 11 (2 juillet 2021) : 5781–91. http://dx.doi.org/10.1007/s11696-021-01755-z.

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AbstractThe aim of the current study was to analyze the stability of rosmarinic acid in ethanolic tinctures of lemon balm (Melissa officinalis L.), oregano (Origanum vulgare L.), peppermint (Mentha x piperita), rosemary (Rosmarinus officinalis L.), sage (Salvia officinalis L.), and thyme (Thymus vulgaris L.). High-performance liquid chromatography with diode-array detection (HPLC–DAD) was employed to monitor the concentration of the marker compound over a six month period. Furthermore, the tinctures were also evaluated for caffeic acid, total phenolic content, and 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity. We observed that the concentration of rosmarinic acid in tincture stored in closed amber glasses at ambient temperature decreased significantly during 6-month storage. Furthermore, our study squarely confirms the fact that a part of rosmarinic acid is converted to caffeic acid. The tested tinctures can be listed in the following order according to the greatest stability of the marker compound: rosemary > peppermint > oregano > lemon balm > thyme > sage. The results of the study indicated a linear relationship between DPPH values and total phenolic (R2 = 0.92) or rosmarinic acid (R2 = 0.85) contents.

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El-Naggar, Hany M., Paul E. Read et Susan L. Cuppett. « (292) The Effect of Darkness and Sucrose Concentrations on the Production of Rosmarinic Acid in the Callus of Five Rosemary Genotypes ». HortScience 41, n^o 4 (juillet 2006) : 1078B—1078. http://dx.doi.org/10.21273/hortsci.41.4.1078b.

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Rosemary Rosmarinus officinalis is a member of the Lamiaceae. Rosmarinic acid (RA) is a very strong antioxidant produced in the chloroplast, and used to protect plant tissues against oxidative stress. A number of investigations showed that the sucrose concentration in the callus growing medium greatly influenced the production of secondary metabolites of the phenylpropanoid pathway such as RA. The aim of this study was to test the effect of elevated sucrose concentrations (2%, 3%, 4%, 5%, and 6% sucrose) and the effect of light and dark treatments on the production of RA in the callus of five different genotypes. The genotypes were Majorca, Rosmarinus officinalis, Pine Scented, Madeline Hill, and APR. It was found that the dark treatment produces more RA than the light treatment in all genotypes, and in all sucrose concentrations. The RA concentration increased with increasing the sucrose concentration from 2%—reaching the highest concentration at 4% and 5% in most genotypes. The RA concentration declined again at 6% sucrose in all genotypes. We concluded that for the extraction of RA from rosemary callus it is preferred to be produced in the dark—this will save energy and will produce more RA than the light treatment. Also it is preferred to use sucrose concentration at 4% for genotypes Rosmarinus officinalis, Pine Scented, and APR; and 3% sucrose for genotype Madeline Hill in the dark condition. While for the light condition, it is preferred to use 5% sucrose with genotypes Majorca, Rosmarinus officinalis, Pine Scented, and Madeline Hill; and 4% sucrose for genotype APR.

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Tawfeeq, Amani A., Monther F. Mahdi, Ibrahim S. Abaas et Ali Hussein Alwan. « Phytochemical and anti bacterial studies of leaves of Rosmarinus officinalis cultivated in Karbala, Iraq. » Al Mustansiriyah Journal of Pharmaceutical Sciences 17, n^o 2 (10 mars 2018) : 9. http://dx.doi.org/10.32947/ajps.v17i2.48.

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Rosmarinus officinalis is one of the important bioactive medicinal plants belongs to labiatae family. The aim is to identifying the essential oil and rosmarinic acid extracted from rosemary leaves and to evaluate its anti bacterial activity. Gas Chromatography-Mass analysis was performed for essential oil of rosemary and HPTLC analysis was done for 90% methanolic extract of rosemary leaves. Rosmarinic acid was detected and The essential oil content was 1.5% and characterized with high amount of 1,8 cineol (53.63%) . The zone of inhibition of some bacteria was estimated. Our findings showed that rosemary leaves has Good percentage of rosemary phytochemicals (essential oil and rosmarinic acid) and has significant antibacterial activity against different species of bacteria.

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Gonçalves, Catarina, Daniela Fernandes, Inês Silva et Vanessa Mateus. « Potential Anti-Inflammatory Effect of Rosmarinus officinalis in Preclinical In Vivo Models of Inflammation ». Molecules 27, n^o 3 (18 janvier 2022) : 609. http://dx.doi.org/10.3390/molecules27030609.

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This systematic review aimed to evaluate the potential anti-inflammatory effect of Rosmarinus officinalis in preclinical in vivo models of inflammation. A search was conducted in the databases PubMed, Scopus, and Web of Science, with related keywords. The inclusion criteria were inflammation, plant, and studies on rats or mice; while, the exclusion criteria were reviews, studies with in vitro models, and associated plants. The predominant animal models were paw edema, acute liver injury, and asthma. Rosemary was more commonly used in its entirety than in compounds, and the prevalent methods of extraction were maceration and hydrodistillation. The most common routes of administration reported were gavage, intraperitoneal, and oral, on a route-dependent dosage. Treatment took place daily, or was single-dose, on average for 21 days, and it more often started before the induction. The most evaluated biomarkers were tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, IL-10, myeloperoxidase (MPO), catalase (CAT), glutathione (GSH), glutathione peroxidase (GPx), malondialdehyde (MDA), and superoxide dismutase (SOD). The best results emerged at a dose of 60 mg/kg, via IP of carnosic acid, a dose of 400 mg/kg via gavage of Rosmarinus officinalis, and a dose of 10 mg/kg via IP of rosmarinic acid. Rosmarinus officinalis L. showed anti-inflammatory activity before and after induction of treatments.

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Ferreira, Luciana Garros, Andrea Carla Celotto, Verena Kise Capellini, Agnes Afrodite Sumarelli Albuquerque, Tales Rubens de Nadai, Marco Tulio Menezes de Carvalho et Paulo Roberto Barbosa Evora. « Does rosmarinic acid underestimate as an experimental cardiovascular drug ? » Acta Cirurgica Brasileira 28, suppl 1 (2013) : 83–87. http://dx.doi.org/10.1590/s0102-86502013001300016.

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PURPOSE: The rationale of the present review is to analize the activity of Rosmarinus officinalis in the the cardiovascular system METHODS: A MEDLINE database search (from January 1970 to December 2011) using only rosmarinic acid as searched term. RESULTS: The references search revealed 509 references about rosmarinic acid in 40 years (the first reference is from 1970). There is a powerful prevalence of antioxidant and cancer studies. Other diseases are few cited, as inflammation, brain (Alzheimer and Parkinson disease) and, memory; allergy; diabetes; atherosclerosis, and; hypertension. It is necessary to consider the complete absence of studies on coronary artery disease, myocardial ischemia, heart failure or ischemia/reperfusion injury. CONCLUSION: Rosmarinic acid is underestimated as an experimental cardiovascular drug and deserves more attention.

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Susilowati, Dyah. « UTILIZATION OF ROSMARIN LEAF OIL (Rosmarinus officinalis L) ON Culex Quinquefasciatus MOSQUITO LARVA AS A FILARIASIS VECTOR (ELEPHANT FOOT DISEASE) ». Jurnal Farmasi (Journal of Pharmacy) 1, n^o 1 (20 octobre 2018) : 27–33. http://dx.doi.org/10.37013/jf.v1i1.60.

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Elephantiasis (filariasis / elephantiasis) is still endemic in hundreds of districts in Indonesia and has become a health problem for the world community in accordance with the resolution of the World Health Assembly (WHA) in 1997. This is caused by Culex quinquefasciatus mosquitoes as one of many filariasis vectors found in urban areas (Gandahusada et al., 1998), while the number of filariasis chronic cases reported until 2009 was 11,914 cases. Filariasis / elephantiasisi is a chronic infectious disease caused by filarial worm infection transmitted by various types of mosquitoes in the lymph nodes, this disease is chronic (chronic) and if it does not get treatment can cause permanent disability in the form of enlargement of the legs, arms and genitals both women and men man. Rosmarin (Rosmarinus officinalis L.) is a multifunctional plant in Indonesia especially abroad, one of its benefits is as larvicides. Because rosmarin has a chemical compound, one of them is essential oil which has larvicidal effectiveness on Culex quinquefasciatus mosquito larvae as vector filariasis (elephantiasis) Essential oil was obtained by distillation of fresh rosmarin leaves, then each extract was made with 1000 ppm stock solution and then made 6 series of concentration of 12.5 ppm to 300 ppm then tested on 20 larvae of instar III Culex quinquefasciatus, Observations were made after 24 hours and calculated LC90 uses an analysis of the orbit. The results obtained were only essential oils of Rosmarin leaves (Rosmarinus officinalis L.) which had larvicidal activity against Culex quinquefasciatus mosquito larvae, with LC90 182,9756 ppm.

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EL-BELTAGI, Hossam S., et Mona H. BADAWI. « Comparison of Antioxidant and Antimicrobial Properties for Ginkgo biloba and Rosemary (Rosmarinus officinalis L.) from Egypt ». Notulae Botanicae Horti Agrobotanici Cluj-Napoca 41, n^o 1 (28 mai 2013) : 126. http://dx.doi.org/10.15835/nbha4118928.

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The widespread use of medicinal plants for health purposes has increased dramatically due to their great importance to the public health. In this study levels of phenolic, flavonoid contents of Ginkgo biloba and Rosmarinus officinalis from Egypt were determined. HPLC was used to identify and quantify the phenolic compounds in selected plants. The plant extracts were evaluated for their antioxidant activities using various antioxidant methodologies, (i) scavenging of free radicals using 2, 2-diphenyl-1-picrylhydrazyl, (ii) metal ion chelating capacity, and (iii) scavenging of superoxide anion radical. The antimicrobial activity of both plantâ€™s extracts were evaluated against a panel of microorganisms by using agar disc diffusion method. The total phenolic content (75.30 and 98.31 mg/g dry weight in G. biloba and R. officinalis, respectively) was significantly (p<0.05) different. Among the identified phenolic compounds, quercetin, kaempferol and caffeic acid were the predominant phenolic compounds in Ginkgo biloba, whereas carnosic acid, rosmarinic acid, narinigen and hispidulin were the predominant phenolic compound in Rosmarinus officinalis leaves. The antioxidant activity increased with the concentration increase. The R. officinalis was more active than G. biloba extract against Gram-negative bacteria. This study reveals that the consumption of these plants would exert several beneficial effects by virtue of their antioxidant and antimicrobial activities.

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Swari, Dewa Ayu Made Adnya, I. Wayan Martadi Santika et I. Gusti Made Aman. « ANTIFUNGAL ACTIVITIES OF ETHANOL EXTRACT OF ROSEMARY LEAF (Rosemarinus officinalis L.) AGAINST Candida albicans ». Journal of Pharmaceutical Science and Application 2, n^o 1 (18 juin 2020) : 28. http://dx.doi.org/10.24843/jpsa.2020.v02.i01.p05.

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Background: This study was a preliminary study aimed to investigating the in vitro activity of rosmarinic acid contained on Rosemary leaves (Rosemarinus officinalis L.) on the Candida albicans. Rosmarinic acid can be an inhibitor of the RTPase enzyme in the fungus Candida albicans. Objective: This study is expected to provide information in the development of science about the antifungal activity of ethanol extract of Rosemary leave (Rosmarinus officinalis L.) againsts C.albicans. The recent research was an laboratory experimental. Experiments on an ethanol extract of Rosemary leaves (Rosemarinus officinalis L.) and post-test design of the control group. Methods: The extraction method used in this study was a multilevel extraction method. The antifungal activity testing method uses discs and MIC methods. Replication was done six times at two concentration namely, 80% (b/v) and 100% for each measurement. Results: The data analysis was carried out using SPSS with 95% of confidence level with the Kruskal Wallis analysis then completed with the Mann Whitney U test. The results of the MIC experiment showed all the various concentrations of ethanol extract performed Candida albicans growth on the surface of the media. Extract with 80% (b/v) concentration showed greater antifungal activity. Furthermore the MIC was shown by 80% (b/v) concentration of the ethanol extract of Rosemary leaves against Candida albicans. The test results of the antifungal activity of the Rosemary leaf ethanol extract showed the presence of inhibitory diameter in the ethanol extract of the Rosemary leaf concentration of 80% (b/v) with a mean ± SD of 10.6 ± 0.57735 and RSD of 5.774%. Conclusion: The ethanol extract of Rosemary leaves (Rosmarinus officinalis L.) has an inhibitory effect on the fungus Candida albicans at a concentration of 80% (b / v) Keywords: Antifungal activity, Candida albicans, Rosemary, RTPase, ethanol extract

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Castañeta, Grover, Nicolas Cifuentes, Beatriz Sepulveda, Daniela Bárcenas-Pérez, José Cheel et Carlos Areche. « Untargeted Metabolomics by Using UHPLC–ESI–MS/MS of an Extract Obtained with Ethyl Lactate Green Solvent from Salvia rosmarinus ». Separations 9, n^o 11 (26 octobre 2022) : 327. http://dx.doi.org/10.3390/separations9110327.

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Salvia rosmarinus (Lamiaceae), previously known as Rosmarinus officinalis, is a plant cultivated worldwide, native to the Mediterranean region. Its leaves are traditionally used for cooking. This species possesses numerous biological activities, including antioxidant, antimicrobial, anticancer, anti-inflammatory, and hepatoprotective properties. These biological properties are due to the presence of phenolic compounds, including rosmarinic acid and phenolic diterpenoids, such as carnosic acid and carnosol. In this study, we investigated the chemical composition of a green extract obtained by maceration with ethyl lactate for the first time. Seventy-five compounds were tentatively identified by UHPLC–ESI–MS/MS, including six organic acids, six cinnamic acid derivatives, five fatty acids, eighteen flavonoids, and thirty-eight terpenoids. Thus, abietane-type diterpenoids from the ethyl lactate extract were the predominant diterpenoids in the Chilean S. rosmarinus species, in contrast to the Chinese species, in which labdane and isopimarane-type diterpenoids were found for the first time. Finally, our study confirms that the extraction of S. rosmarinus with green ethyl lactate as a solvent is efficient and sustainable for the identification of flavonoids, phenols, and terpenoids from leaves.

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Moghadam, Ali, Eisa Foroozan, Ahmad Tahmasebi, Mohammad Sadegh Taghizadeh, Mohammad Bolhassani et Morteza Jafari. « System network analysis of Rosmarinus officinalis transcriptome and metabolome—Key genes in biosynthesis of secondary metabolites ». PLOS ONE 18, n^o 3 (2 mars 2023) : e0282316. http://dx.doi.org/10.1371/journal.pone.0282316.

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Medicinal plants contain valuable compounds that have attracted worldwide interest for their use in the production of natural drugs. The presence of compounds such as rosmarinic acid, carnosic acid, and carnosol in Rosmarinus officinalis has made it a plant with unique therapeutic effects. The identification and regulation of the biosynthetic pathways and genes will enable the large-scale production of these compounds. Hence, we studied the correlation between the genes involved in biosynthesis of the secondary metabolites in R. officinalis using proteomics and metabolomics data by WGCNA. We identified three modules as having the highest potential for the metabolite engineering. Moreover, the hub genes highly connected to particular modules, TFs, PKs, and transporters were identified. The TFs of MYB, C3H, HB, and C2H2 were the most likely candidates associated with the target metabolic pathways. The results indicated that the hub genes including Copalyl diphosphate synthase (CDS), Phenylalanine ammonia lyase (PAL), Cineole synthase (CIN), Rosmarinic acid synthase (RAS), Tyrosine aminotransferase (TAT), Cinnamate 4-hydroxylase (C4H), and MYB58 are responsible for biosynthesis of important secondary metabolites. Thus, we confirmed these results using qRT-PCR after treating R. officinalis seedlings with methyl jasmonate. These candidate genes may be employed for genetic and metabolic engineering research to increase R. officinalis metabolite production.

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Tawfeeq, Amani A., Monther F. Mahdi, Ibrahim S. Abaas et Ali Hussein Alwan. « ISOLATION, QUANTIFICATION, AND IDENTIFICATION OF ROSMARINIC ACID, GAS CHROMATOGRAPHY-MASS SPECTROMETRY ANALYSIS OF ESSENTIAL OIL, CYTOTOXIC EFFECT, AND ANTIMICROBIAL INVESTIGATION OF ROSMARINUS OFFICINALIS LEAVES. » Asian Journal of Pharmaceutical and Clinical Research 11, n^o 6 (7 juin 2018) : 126. http://dx.doi.org/10.22159/ajpcr.2018.v11i6.24134.

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Objective: Rosmarinus officinalis L. is an aromatic perennial herb with fragrant evergreen needle-like leaves, and it is member species of Lamiaceae family raised from Mediterranean region. The aims of the study were isolation, quantification, and identification of rosmarinic acid of R. officinalis leaves and essential oil analysis using various chromatographic and spectroscopic methods, and also cytotoxic and antibacterial investigation against different species of bacteria.Methods: It was isolated by preparative HPLC and preparative TLC, and then it was determined by HPTLC. The identification and the structural elucidation of isolated rosmarinic acid were performed by H-nuclear magnetic resonance, electrospray ionization mass spectrometry (MS), infrared, and ultraviolet. Essential oil was analyzed by Gas Chromatography/Mass.Results: Results highlighted that rosmarinic acid content was 0.9% and the oil content was 1.8%, and R. officinalis chemotypes of Iraqi rosemary oil were camphor 23.04%, 1, 8-cineole 14.01%, and terpinen-4-ol 13.8%. The rosemary chemotype characterized as a high concentration of terpinen- 4-ol and good inhibition effect of rosemary methanolic extract against different species bacteria: Enterococcus faecalis, Staphylococcus saprophyticus, Acinetobacter baumannii, and Proteus mirabilis.Conclusions: The plant has a good content of rosemary phytochemicals and antibacterial effect, so the plantation of rosemary in Iraq has been successes. These isolated compounds are a suitable candidate for further clinical and pharmacological study.

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Delueg, Stefanie, Christian G. Kirchler, Florian Meischl, Yukihiro Ozaki, Michael A. Popp, Günther K. Bonn et Christian W. Huck. « At-Line Monitoring of the Extraction Process of Rosmarini Folium via Wet Chemical Assays, UHPLC Analysis, and Newly Developed Near-Infrared Spectroscopic Analysis Methods ». Molecules 24, n^o 13 (6 juillet 2019) : 2480. http://dx.doi.org/10.3390/molecules24132480.

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The present study demonstrates the applicability of at-line monitoring of the extraction process of Rosmarinus officinalis L. leaves (Rosmarini folium) and the development of near-infrared (NIR) spectroscopic analysis methods. Therefore, whole dried Rosmarini folium samples were extracted by maceration with 70% (v/v) ethanol. For the experimental design three different specimen-taking plans were chosen. At first, monitoring was carried out using three common analytical methods: (a) total hydroxycinnamic derivatives according to the European Pharmacopoeia, (b) total phenolic content according to Folin–Ciocalteu, and (c) rosmarinic acid content measured by UHPLC-UV analysis. Precision validation of the wet chemical assays revealed a repeatability of (a) 0.12% relative standard deviation (RSD), (b) 1.1% RSD, and (c) 0.28% RSD, as well as an intermediate precision of (a) 4.1% RSD, (b) 1.3% RSD, and (c) 0.55% RSD. The collected extracts were analyzed with a NIR spectrometer using a temperature-controlled liquid attachment. Samples were measured in transmission mode with an optical path length of 1 mm. The combination of the recorded spectra and the previously obtained analytical reference values in conjunction with multivariate data analysis enabled the successful establishment of partial least squares regression (PLSR) models. Coefficients of determination (R2) were: (a) 0.94, (b) 0.96, and (c) 0.93 (obtained by test-set validation). Since Pearson correlation analysis revealed that the reference analyses correlated with each other just one of the PSLR models is required. Therefore, it is suggested that PLSR model (b) be used for monitoring the extraction process of Rosmarini folium. The application of NIR spectroscopy provides a fast and non-invasive alternative analysis method, which can subsequently be implemented for on- or in-line process control.

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El-Naggar, Hany M., Paul E. Read et Ayed Al-Abdallat. « (293) The PAL Gene Activity in Five Rosemary Genotypes and Its Effect on the Production of Rosmarinic Acid ». HortScience 41, n^o 4 (juillet 2006) : 1078C—1078. http://dx.doi.org/10.21273/hortsci.41.4.1078c.

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Phenylalanine ammonia-lyase (PAL) enzyme is the most extensively studied enzyme in the phenylpropanoid pathway. Studies on the biosynthesis of rosmarinic acid (RA) showed that the PAL enzyme catalyzes the initial step of the phenylpropanoid pathway. The increase in RA content in plant tissues in vitro coincided with the increase in PAL activity. The aim of this study was to investigate the activity of the gene responsible for the production of the PAL enzyme in the five rosemary genotypes; this will give more understanding about the accumulation of rosmarinic acid in the five rosemary genotypes. The genotypes were Majorca, Rosmarinus officinalis, Pine Scented, Madeline Hill and APR. Northern blot hybridization between the PAL gene primer and the five genotypes' cDNA showed bands at 300 bp in all the five genotypes for the PAL gene. The expression of the PAL gene was high in genotypes Majorca, Rosmarinus officinalis, and Madeline Hill, while the expression was low in genotypes Pine Scented and APR. It was expected that the genotypes having the highest PAL gene expression will produce the highest amount of RA, but the highest genotype in PAL gene expression Madeline Hill had the lowest RA production in their leaves. This could occur due to the tissue specific regulation inside plant tissues. Inside the callus tissues, where the specific tissue regulation no longer exists, the RA was produced in repetitively large amounts in genotypes with high PAL gene expression.

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Ghasemian, Mona, Sina Owlia et Mohammad Bagher Owlia. « Review of Anti-Inflammatory Herbal Medicines ». Advances in Pharmacological Sciences 2016 (2016) : 1–11. http://dx.doi.org/10.1155/2016/9130979.

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Medicinal plants and their secondary metabolites are progressively used in the treatment of diseases as a complementary medicine. Inflammation is a pathologic condition that includes a wide range of diseases such as rheumatic and immune-mediated conditions, diabetes, cardiovascular accident, and etcetera. We introduce some herbs which their anti-inflammatory effects have been evaluated in clinical and experimental studies.Curcuma longa, Zingiber officinale, Rosmarinus officinalis, Borago officinalis, evening primrose, and Devil’s claw are some of the introduced medicinal herbs in this review. Since the treatment of inflammation is not a one-dimensional remedy, this review tries to reach a multidimensional therapeutic approach to inflammation with the help of herbal medicine and modification in lifestyle.

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Moliner, Cristina, Víctor López, Lillian Barros, Maria Inês Dias, Isabel C. F. R. Ferreira, Elisa Langa et Carlota Gómez-Rincón. « Rosemary Flowers as Edible Plant Foods : Phenolic Composition and Antioxidant Properties in Caenorhabditis elegans ». Antioxidants 9, n^o 9 (1 septembre 2020) : 811. http://dx.doi.org/10.3390/antiox9090811.

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Rosmarinus officinalis L., commonly known as rosemary, has been largely studied for its wide use as food ingredient and medicinal plant; less attention has been given to its edible flowers, being necessary to evaluate their potential as functional foods or nutraceuticals. To achieve that, the phenolic profile of the ethanolic extract of R. officinalis flowers was determined using LC-DAD-ESI/MSn and then its antioxidant and anti-ageing potential was studied through in vitro and in vivo assays using Caenorhabditis elegans. The phenolic content was 14.3 ± 0.1 mg/g extract, trans rosmarinic acid being the predominant compound in the extract, which also exhibited a strong antioxidant capacity in vitro and increased the survival rate of C. elegans exposed to lethal oxidative stress. Moreover, R. officinalis flowers extended C. elegans lifespan up to 18%. Therefore, these findings support the potential use of R. officinalis flowers as ingredients to develop products with pharmaceutical and/or nutraceutical potential.

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Kakouri, Eleni, Olti Nikola, Charalabos Kanakis, Kyriaki Hatziagapiou, George I. Lambrou, Panayiotis Trigas, Christina Kanaka-Gantenbein et Petros A. Tarantilis. « Cytotoxic Effect of Rosmarinus officinalis Extract on Glioblastoma and Rhabdomyosarcoma Cell Lines ». Molecules 27, n^o 19 (26 septembre 2022) : 6348. http://dx.doi.org/10.3390/molecules27196348.

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Rosmarinus officinalis is a well-studied plant, known for its therapeutic properties. However, its biological activity against several diseases is not known in detail. The aim of this study is to present new data regarding the cytotoxic activity of a hydroethanolic extract of Rosmarinus officinalis on glioblastoma (A172) and rhabdomyosarcoma (TE671) cancer cell lines. The chemical composition of the extract is evaluated using liquid chromatography combined with time-of-flight mass spectrometry, alongside its total phenolic content and antioxidant activity. The extract showed a promising time- and dose-dependent cytotoxic activity against both cell lines. The lowest IC50 values for both cell lines were calculated at 72 h after treatment and correspond to 0.249 ± 1.09 mg/mL for TE671 cell line and 0.577 ± 0.98 mg/mL for A172 cell line. The extract presented high phenolic content, equal to 35.65 ± 0.03 mg GAE/g of dry material as well as a strong antioxidant activity. The IC50 values for the antioxidant assays were estimated at 12.8 ± 2.7 μg/mL (DPPH assay) and 6.98 ± 1.9 μg/mL (ABTS assay). The compound detected in abundance was carnosol, a phenolic diterpene, followed by the polyphenol rosmarinic acid, while the presence of phenolic compounds such as rhamnetin glucoside, hesperidin, cirsimaritin was notable. These preliminary results suggest that R. officinalis is a potential, alternative source of bioactive compounds to further examine for abilities against glioblastoma and rhabdomyosarcoma.

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Ramírez-Condoy, Oliver, Lourdes Jerves-Andrade, Andrea Abril et Marcela Redrován-Berrezueta. « Determinación de la actividad antibacteriana de extractos de plantas medicinales frente a Enterococcus faecalis ». MASKANA 13, n^o 2 (30 juin 2022) : 52–57. http://dx.doi.org/10.18537/mskn.13.01.07.

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Enterococcus faecalis es un microorganismo considerado como agente etiológico de diferentes procesos infecciosos, tales como: infecciones de la piel, osteomielitis, neumonía, infecciones del tracto urinario, endocarditis, entre otros, siendo los pacientes nosocomiales una población más vulnerable a esta bacteria. El objetivo del presente estudio fue evaluar la actividad antibacteriana in vitro de extractos metanólicos y clorofórmicos de las plantas Peperomia galioides, Otholobium mexicanum, Zingiber officinale y Rosmarinus officinalis frente a Enterococcus faecalis ATCC 29212, Enterococcus faecalis ATCC 51299 (resistente a la vancomicina) y una cepa clínica de Enterococcus faecalis. Para la evaluación de esta bioactividad se utilizó la metodología de microdilución y puntos de corte establecidos por la CLSI (2021) para Enterococcus faecalis y los resultados obtenidos se expresaron en IC50. El IC50 de mayor interés se obtuvo con el extracto clorofórmico de Otholobium mexicanum frente a la cepa clínica, siendo de 2.050 μg/mL, considerándose como muy activo. El IC50 más destacable de Peperomia galioides se obtuvo con el extracto clorofórmico frente a la cepa clínica con un valor de 8.248 μg/mL, considerándolo como ligeramente activo. Para Rosmarinus officinalis, solo el extracto metanólico mostró una ligera actividad antibacteriana con un IC50 de 20.234 μg/mL frente a Enterococcus faecalis ATCC 29212. Finalmente, todos los extractos de Zingiber officinale no mostraron actividad frente a las concentraciones probadas de las diferentes cepas de Enterococcus faecalis. Los resultados obtenidos son de gran relevancia ya que pueden ser aplicados para la producción de antimicrobianos a partir de fuentes naturales que podrían tener aplicación dentro de la industria farmacéutica, veterinaria o agrícola.

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Dal Pozzo, Marcelo, Julio Viégas, Deise Flores Santurio, Luana Rossatto, Isaura Helena Soares, Sydney Hartz Alves et Mateus Matiuzzi da Costa. « Atividade antimicrobiana de óleos essenciais de condimentos frente a Staphylococcus spp isolados de mastite caprina ». Ciência Rural 41, n^o 4 (9 mars 2011) : 667–72. http://dx.doi.org/10.1590/s0103-84782011005000029.

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Avaliou-se a atividade antimicrobiana dos óleos essenciais (OEs) de Origanum vulgare (orégano), Thymus vulgaris (tomilho), Lippia graveolens (lípia), Zingiber officinale (gengibre), Salvia officinalis (sálvia), Rosmarinus officinalis (alecrim) e Ocimum basilicum (manjericão), bem como de frações majoritárias carvacrol, timol, cinamaldeído e cineol frente a 33 isolados de Staphylococcus spp oriundos de rebanhos leiteiros caprinos. A concentração inibitória mínima (CIM) e a concentração bactericida mínima (CBM) foram determinadas por meio da técnica de microdiluição em caldo. Observou-se atividade antimicrobiana para os OEs de orégano, lípia e tomilho, bem como para as frações majoritárias de carvacrol, timol e cinamaldeído. A ordem decrescente de atividade foi orégano = tomilho > lípia. As frações majoritárias carvacrol, timol e cinamaldeído evidenciaram melhor atividade do que os óleos essenciais e, dentre elas, carvacrol e cinamaldeído foram mais ativas que o timol.

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Samajdar, Saptarshi, et Malay Kr Samanta. « MANAGEMENT OF DIABETES MELLITUS WITH TRADITIONAL AYURVEDIC HERBS : A REVIEW ». Journal of Advanced Scientific Research 13, n^o 07 (31 août 2022) : 26–33. http://dx.doi.org/10.55218/jasr.202213702.

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Ayurvedic herbs have been used as a source of medicine since the dawn of humanity. There are over 21,000 herb speciesin India, and thousands of them have been claimed to have therapeutic characteristics. Ayurvedic herbs referenced inancient literature or used historically for diabetes have been found to have anti-diabetic properties in studies undertakenin the last several decades. The current research examines ten such herbs that have been utilized in traditional medicineand have shown anti-diabetic effects in both experimental and clinical studies. The herbs mentioned in this review areCeratonia silliqua, Syzygium aromaticum, Verbena officinalis, Abelmoschus esculentus, Allium cepa, Coriandrum sativum, Lepidiumsativum, Rosmarinus officinalis, Zingiber officinale, Eupatorium sp, Mangifera indica, Swertia chirayta, Ocimum sanctum,Azadirachta indica and Ablemoschus moschatus. All these important ayurvedic plants have shown good responses againstdiabetes mellitus.

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Lucarini, Rodrigo, Wagner Bernardes, Marcos Tozatti, Ademar Filho, Marcio Silva, Claudia Momo, Antonio Crotti, Carlos Martins et Wilson Cunha. « Hepatoprotective effect of Rosmarinus officinalis and rosmarinic acid on acetaminophen-induced liver damage ». Emirates Journal of Food and Agriculture 26, n^o 10 (2014) : 878. http://dx.doi.org/10.9755/ejfa.v26i10.17836.

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Saad, Engy M., Nesrine Abdelrehim El Gohary, Mohammad Abdel-Halim, Heba Handoussa, Rasha Mohamed El Nashar et Boris Mizaikoff. « Molecularly imprinted polymers for selective extraction of rosmarinic acid from Rosmarinus officinalis L. » Food Chemistry 335 (janvier 2021) : 127644. http://dx.doi.org/10.1016/j.foodchem.2020.127644.

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Ben Kaab, Sofiene, Iness B. Rebey, Marwa Hanafi, Chadi Berhal, Marie L. Fauconnier, Caroline De Clerck, Riadh Ksouri et Haissam Jijakli. « Rosmarinus officinalis essential oil as an effective antifungal and herbicidal agent ». Spanish Journal of Agricultural Research 17, n^o 2 (26 juillet 2019) : e1006. http://dx.doi.org/10.5424/sjar/2019172-14043.

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In order to reduce the use of chemical pesticides, great interest has been focused on environment-friendly biological control agents and botanicals that preserve biodiversity. In this context, our study aimed to assess the antifungal and herbicidal activities of Rosmarinus officinalis essential oil (EO) to find an alternative to synthetic pesticides. The chemical composition of R. officinalis essential oil was determined by gaz chromatography-mass spectrometry analysis (GC-MS). Results showed that R. officinallis EO was rich in monoterpenes and the major constituents were 1,8-cineole (54.6%), camphor (12.27%) and α-pinene (7.09%). However, under laboratory conditions, two tests were carried out. The first one consisted on the study of EO antifungal activity using ELISA microplates and the second one consisted on evaluating the effect of EO on seedling growth of weeds. It was confirmed that this EO significantly inhibits spore germination of Fusarium oxysporum, Fusarium culmorum, Penicillium italicum and at 6 mM, the percentage of inhibition reached 100% on Fusarium oxysporum. Indeed, EO slows down seedling growth of Trifolium incarnatum, Silybum marianum, and Phalaris minor. In fact, EO at 5 mM completely inhibits seed germination. On the other hand, another experiment was carried out to evaluate the herbicidal activity by spraying EO on weeds. This showed that a novel herbicide formulation was set up for the first time to improve the activity of R. officinalis EO on post-emergence. Overall, R. officinalis EO can be suggested as a potential eco-friendly pesticide and suitable source of natural compounds potentially usable as natural pesticides.

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Salokhe, Shivani Shashikant, S. U. Kolhe, S. V. Tembhurne et Dipali Hanmghar. « 28 Days repeated oral toxicity study of Rosmarinus officinalis in Wistar Rats ». Journal of Drug Delivery and Therapeutics 10, n^o 6-s (15 décembre 2020) : 67–74. http://dx.doi.org/10.22270/jddt.v10i6-s.4616.

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Rosmarinus officinalis has long been used as a traditional oriental medicine. It is traditionally used as antioxidants as well as essential oil. Rosmarinus officinalis mainly contains the phenolic compounds which is responsible for the antioxidant property. In the present study, 28-day subacute oral dose toxicity studies of hydroalcoholic extracts of the plant of Rosmarinus officinalis were performed in Wistar rats. The repeated oral toxicity study was carried out to detect the no-observed adverse effect level (NOAEL). In this study, a total of 48 rats were classified into the control, low dose (300 mg/kg), medium dose (500 mg/kg) and high dose (1000 mg/kg) treatment groups. The extract was administered daily from day 1 until day 28. At the end of the study, the animals were humanely sacrificed and assessed for the effect extract of Rosmarinus officinalis plant on body weight and relative organ weights, biochemical, haematological and histopathological parameters. The biochemical parameters for the assessment of kidney and liver injuries were carried out. Results of haematological and biochemistry results showed no changes in the control and treated groups. In the histopathology, evaluation of kidney tissues in all treated groups showed no significant (p > 0.05) lesions. The results conclude that hydro-alcoholic extract of leaves Rosmarinus officinalis was found to be safe at highest dose level of 1000mg/kg for 28 days of oral administration. Keywords: Repeated toxicity study, Rosmarinus officinalis, biochemical analysis, histopathological study

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Meireles, M. A. A. « Extração Supercrítica : Aspectos Técnicos e Econômicos ». Revista Fitos 2, n^o 01 (1 juin 2006) : 65–72. http://dx.doi.org/10.32712/2446-4775.2006.42.

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Este trabalho discute alguns aspectos técnicos e econômicos da extração supercrítica. As principais etapas para o desenvolvimento do processo são discutidas em termos da elaboração de extratos de macela (Achyrocline alata e Achyrocline satureioides) e cravo-da-índia (Eugenia caryophyllus). Dados de processo tais como a temperatura, a pressão e o rendimento são apresentados para a obtenção de extratos de pimenta-do-reino (Piper nigrum), capim limão (Cymbopogon citratus), bacuri (Platonia insignis), recuperação de carotenóides de resíduos das fibras de dendê (Elaeis guineensis), Artemísia (Artemisia annua), erva-baleeira (Cordia verbenacea), funcho (Foeniculum vulgare), alecrim (Rosmarinus officinalis), gengibre (Zingiber officinale), erva-doce (Pimpinela anisum), etc..

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Velamuri, Ravikishore, Yashaswini Sharma, John Fagan et Jim Schaefer. « Application of UHPLC-ESI-QTOF-MS in Phytochemical Profiling of Sage (Salvia officinalis) and Rosemary (Rosmarinus officinalis) ». Planta Medica International Open 07, n^o 04 (novembre 2020) : e133-e144. http://dx.doi.org/10.1055/a-1272-2903.

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AbstractUHPLC with QTOF-MS is widely used as a powerful tool for metabolomic analysis. This technology has recently been applied to the analysis of polyphenols in food and herb extracts. Sage (Salvia officinalis) and rosemary (Rosmarinus officinalis), belonging to the family Lamiaceae, are known for their potent antioxidant properties due to the presence of polyphenols. We have developed a sensitive and reproducible UHPLC-QTOF-MS/MS-based method for comprehensive phytochemical profiling and the identification and quantitation of specific polyphenolic compounds present in sage and rosemary leaves. The herbs were extracted ultrasonically using methanol as the solvent. In sage, rosmarinic acid (17 678.7±673.4 µg/g) and 12-methoxy carnosic acid (21 918.3±715.4 µg/g) were found in the highest concentrations among all polyphenols. In contrast, rosmarinic acid (14 311.0±636.4 µg/g), luteolin-3'-acetyl-O-glucuronide (1488.50±47.58 µg/g), and luteolin-7-O-glucuronide (1053.68±68.83 µg/g) were observed in the highest concentrations in rosemary. Sagerinic acid, rosmanol, rosmadial, carnosol, and carnosic acid were found in abundance in both sage and rosemary. The pentacyclic triterpenoid, corosolic acid ([M - H]¯ m/z 471.35), was detected for the first time in both plants. Of the 47 polyphenolic compounds identified in each plant, 38 compounds were found in common in rosemary and sage. A flavonoid compound, baicalin ([M -H]¯ m/z 445.08), was identified for the first time in S. officinalis. Also, pectolinarigenin ([M - H]¯ m/z 313.07), a dimethoxyflavone, was detected for the first time in both sage and rosemary leaves.

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S. M. Cutrim, Elaine, Amanda M. Teles, Adenilde N. Mouchrek, Victor E. Mouchrek Filho et Gustavo O. Everton. « Evaluation of Antimicrobial and Antioxidant Activity of Essential Oils and Hydroalcoholic Extracts of Zingiber officinale (Ginger) and Rosmarinus officinalis (Rosemary) ». Revista Virtual de Química 11, n^o 1 (2019) : 60–81. http://dx.doi.org/10.21577/1984-6835.20190006.

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Chrpová, D., L. Kouřimská, M. H. Gordon, V. Heřmanová, I. Roubíčková et J. Pánek. « Antioxidant activity of selected phenols and herbs used in diets for medical conditions ». Czech Journal of Food Sciences 28, No. 4 (6 septembre 2010) : 317–25. http://dx.doi.org/10.17221/129/2010-cjfs.

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The antioxidant capacity of some herbs used in dietology practice was determined by the DPPH free radical method, which was calibrated with ascorbic acid. Partially hydrophilic phenolic compounds are the most active compounds in plants, and therefore water was used as the extraction agent. Besides antioxidant capacity, the content of total phenolic compounds was also measured and a strong correlation between these two variables was found. The extracts of lemon balm (Melissa officinalis L.), peppermint (Mentha x piperita L.), oregano (Origanum vulgare L.), Greek oregano (Origanum heracleoticum L.), sage (Salvia officinalis L.) and winter savory (Satureja montana L.) showed very significant activity. It was comparable with the activity of green tea in the case of oregano and peppermint. Lower activity was observed in the case of rosemary (Rosmarinus officinalis L.), marjoram (Majorana hortensis), hyssop (Hyssopus officinalis L.), sweet basil (Ocimum basilicum), and lovage (Levisticum officinale Koch.). The inhibitory activity of the herb extracts was monitored also during the autooxidation of lard. Very high antioxidant capacity was observed mainly in sage samples, but also in marjoram and Greek oregano. The extracts of peppermint, oregano, rosemary, winter savory, lemon balm and hyssop showed middle activity comparable to that of α-tocopherol. The antioxidant capacity of sweet basil and lovage was insignificant.

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Pistelli, Laura, Silvia Giovanelli, Francesca D'Angiolillo, Kalina Karkleva, Michele Leonardi, Katarzyna Ambryszewska, Claudio Cervelli et Luisa Pistelli. « Antioxidant Activity of Several Essential Oils from Different Rosmarinus officinalis Cultivars Grown in Sanremo (Italy) ». Natural Product Communications 13, n^o 9 (septembre 2018) : 1934578X1801300. http://dx.doi.org/10.1177/1934578x1801300917.

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The evaluation of the essential oil (EO) profile of several cultivars of Rosmarinus officinalis, usually not used for food, and the assessment of their antioxidant activity as potential sources of natural antioxidants were studied in this work. Five different cultivars of Rosmarinus officinalis: R. officinalis ‘Alba'; R. officinalis ‘Blue Rain'; R. officinalis ‘Corsican Blue'; R. officinalis ‘Israeli'; R. officinalis ‘Majorca Pink’ and one hybrid of rosemary, Rosmarinus x lavandulaceus Noë, cultivated at CREA Sanremo (Italy), were hydrodistilled separately and the obtained EOs were analysed by GC-MS. Monoterpenes predominated in all the EO profiles but oxygenated monoterpenes were the most abundant constituents in R. officinalis ‘Blue Rain’, R. officinalis ‘Corsican Blue’, R. officinalis ‘Israeli’ and R. officinalis ‘Majorca Pink’. R. x lavandulaceus Noë and R. officinalis ‘Alba’ showed percentages of monoterpene hydrocarbons and oxygenated monoterpenes very similar. α-pinene, camphene, 1,8-cineole, camphor, borneol, verbenone, isobornyl acetate and β-caryophyllene were the main constituents present in all the plant cultivars, even though in different percentages. Myrcene predominate in R. officinalis ‘Alba’ (30.3 %), 1,8 cineol in R. officinalis ‘Blue Rain’ and R. officinalis ‘Israeli’ (21.9% and 26.4%, respectively), while camphor was the main constituent in R. officinalis ‘Majorca Pink’ (42.5%) and in R. x lavandulaceus (24.2%). R. officinalis ‘Corsican Blue’ showed similar amount of the three main components, verbenone, camphor and isobornyl acetate (18.5% >18.2% >15.4%, respectively). All the EOs were also tested for their antioxidant activity using the ferric reducing antioxidant power (FRAP) method and the stable radical DPPH. R. x lavandulaceus Noë showed the highest antioxidant activity, two folds more than that observed in R. officinalis ‘Israeli’.

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BEN JEMAA, Mariem, Mejdi SNOUSSI, Hanen FALLEH, Raja SERAIRI BEJI et Riadh KSOURI. « Phytochemical components and antioxidant and antimicrobial activities of essential oils from native Tunisian Thymus capitatus and Rosmarinus officinalis ». Nutrition & ; Santé 10, n^o 01 (30 juin 2021) : 62–71. http://dx.doi.org/10.30952/ns.10.1.8.

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Introduction. Essential oils and their components are currently of great interest as a potential source of highly bioactive natural molecules. They are being studied for their possible use as safe alternative for food protection against oxidation and microbial spoilage. Objective. This study aimed on the phytochemical prospection of Thymus capitatus and Rosmainus officinalis essential oils and their oral toxicity evaluation. Material and methods. Chemical analysis of tested essential oils was carried out using gas chromatography combined to mass spectroscopic (GC-MS). Their safety limit was evaluated by acute toxicity. The antioxidant activity was estimated using in vitro methods. The antimicrobial activity was evaluated against twelve pathogenic germs. Results. Results showed that carvacrol and 1,8-cineol were the major compounds of Thymus capitatus and Rosmarinus officinalis essential oils. Acute toxicity results exhibited that both tested essential oils were inoffensive at 2000 mg/kg. Additionally, Thymus capitatus essential oil presented higher antioxidant activity than Rosmarinus officinalis: 2,2-diphényl-1-picrylhydrazyl (DPPH) assay results showed lower IC50 for Thymus capitatus essential oil than Rosmarinus officinalis. Concerning the antimicrobial results, Thymus capitatus essential oil presented greater efficacy than R. officinalis. Indeed, the minimal growth inhibition diameter generated by thyme essential oil exceeded 38 mm (except for Salmonella typhirium) and reached 60 mm (against C. tropicalis and C. albicans). However, the maximal growth inhibition diameter generated by R. officinalis essential oil was limited to 36 mm (against Shigella sonnei). Conclusion. Overall, Thymus capitatus and Rosmarinus officinalis essential oils have strong potential applicability for pharmaceutical industries.

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Lazarević, Miloš, Maja Milošević, Nada Petrović, Slobodan Petrović, Giuseppe Damante, Jelena Milašin et Branislav Milovanović. « Cytotoxic Effects of Different Aromatic Plants Essential Oils on Oral Squamous Cell Carcinoma- an in vitro Study ». Balkan Journal of Dental Medicine 23, n^o 2 (1 juillet 2019) : 73–79. http://dx.doi.org/10.2478/bjdm-2019-0014.

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Summary Background/Aim: Current approaches in therapy of head and neck cancers are surgery, radiotherapy and chemotherapy. However, recurrence, development of multidrug resistance, side effects, and high costs of therapy are significant problems which point to the need for more efficient and less toxic drugs and interventions. Material and Methods: Eight essential oils obtained from Thymus serpyllum, Mentha piperita, Juniperus communis, Rosmarinus officinalis, Melissa officinalis, Achillea millefolium, Zingiber officinale, and Helichrysum arenarium were tested for their anti-proliferative on oral squamous cell carcinoma (OSCC) culture and SCC-25 cell line. Cytotoxicity assays (MTT and Neutral red) were used to detect the effect of the mentioned essential oils. Results: T. serpyllum, M. piperita, J. communis, and R. officinalis essential oils exhibited the best anti-proliferative effect, on both types of cells. M. piperita had the greatest effect on SCC-25 cell line (4,5% of viable cells) and OSCC cells (7,2% of viable cells). Overall, cytotoxicity was higher in OSCC than in SCC-25 cell line. Conclusions: This study showed a clear anti-proliferative effect of four essential oils, in vitro making them novel potential antineoplastic agents.

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Armitage, Allan M., James Garner et Jimmy S. Greer. « Rosmarinus officinalis `Athens Blue Spires' ». HortScience 39, n^o 7 (décembre 2004) : 1789. http://dx.doi.org/10.21273/hortsci.39.7.1789.

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Okamura, Nobuyuki, Hiroyuki Haraguchi, Kensuke Hashimoto et Akira Yagi. « Flavonoids in Rosmarinus officinalis leaves ». Phytochemistry 37, n^o 5 (novembre 1994) : 1463–66. http://dx.doi.org/10.1016/s0031-9422(00)90434-5.

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Cordeiro, A. M. T. M., M. L. Medeiros, N. A. Santos, L. E. B. Soledade, L. F. B. L. Pontes, A. L. Souza, N. Queiroz et A. G. Souza. « Rosemary (Rosmarinus officinalis L.) extract ». Journal of Thermal Analysis and Calorimetry 113, n^o 2 (7 novembre 2012) : 889–95. http://dx.doi.org/10.1007/s10973-012-2778-4.

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Chatterjee, Arnab, Sudeep Tandon et Ateeque Ahmad. « Comparative Extraction and Downstream Processing Techniques for Quantitative Analysis of Rosmarinic Acid in Rosmarinus officinalis ». Asian Journal of Chemistry 26, n^o 14 (2014) : 4313–18. http://dx.doi.org/10.14233/ajchem.2014.16266.

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Bellumori, Maria, Marzia Innocenti, Federica Congiu, Gabriele Cencetti, Aida Raio, Felicia Menicucci, Nadia Mulinacci et Marco Michelozzi. « Within-Plant Variation in Rosmarinus officinalis L. Terpenes and Phenols and Their Antimicrobial Activity against the Rosemary Phytopathogens Alternaria alternata and Pseudomonas viridiflava ». Molecules 26, n^o 11 (5 juin 2021) : 3425. http://dx.doi.org/10.3390/molecules26113425.

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This study investigated within-plant variability of the main bioactive compounds in rosemary (Rosmarinus officinalis L.). Volatile terpenes, including the enantiomeric distribution of monoterpenes, and phenols were analyzed in young and mature foliar, cortical and xylem tissues. In addition, antimicrobial activity of rosmarinic acid and selected terpenes was evaluated against two rosemary pathogens, Alternaria alternata and Pseudomonas viridiflava. Data showed that total concentration and relative contents of terpenes changed in relation to tissue source and age. Their highest total concentration was observed in the young leaves, followed by mature leaves, cortical and xylem tissues. Rosmarinic acid and carnosic acid contents did not show significant differences between leaf tissues of different ages, while young and mature samples showed variations in the content of four flavonoids. These results are useful for a more targeted harvesting of rosemary plants, in order to produce high-quality essential oils and phenolic extracts. Microbial tests showed that several terpenes and rosmarinic acid significantly inhibited the growth of typical rosemary pathogens. Overall, results on antimicrobial activity suggest the potential application of these natural compounds as biochemical markers in breeding programs aimed to select new chemotypes less susceptible to pathogen attacks, and as eco-friendly chemical alternatives to synthetic pesticides.

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Ramalingam, Ashwin Kumar, Smiline Girija Aseervatham Selvi et Vijayashree Priyadharsini Jayaseelan. « Targeting prolyl tripeptidyl peptidase from Porphyromonas gingivalis with the bioactive compounds from Rosmarinus officinalis ». Asian Biomedicine 13, n^o 5 (4 juin 2020) : 197–203. http://dx.doi.org/10.1515/abm-2019-0061.

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AbstractBackgroundComplications in periodontitis and other systemic infections related to Porphyromonas gingivalis poses a serious impediment in the treatment process. This leads to the search of novel target proteins to develop newer drugs against P. gingivalis. Prolyl tripeptidyl peptidase (ptp-A) seem to be a vital protein in P. gingivalis virulence and can be a good target for the novel natural bioactive compounds.ObjectivesTo explore the inhibitory potential of Rosmarinus officinalis biocompounds against the ptp-A of P. gingivalis.MethodsThree-dimensional structure of ptp-A was retrieved from the Protein Data Bank with further optimization of both the protein and ligands. In silico inhibitory potential of the selected ligands against ptp-A was done by AutoDock 2.0 and was visualized with Biovia discovery studio visualizing tool with the assessment of the molecular properties of the ligands against ptp-A by molinspiration calculations and drug likeliness.ResultsHigh ptp-A inhibitory effect was observed using rosmarinic acid and luteolin with a bonding energy of −9.81 kcal/mol with 10 hydrogen bond interactions and −9.99 kcal/mol with 7 hydrogen bond interactions, respectively. Carnosic acid and p-coumaric acid showed a binding energy of −7.14 kcal/mol and −6.34 kcal/mol, respectively, with 5 hydrogen bond interactions. Molinspiration assessments showed R. officinalis compounds as the best drug candidates with the topological polar surface area scores <140 Å toward the best oral bioavailability.ConclusionThe carnosic acid, rosmarinic acid, p-coumaric acid, and luteolin from R. officinalis seem to possess a promising inhibitory effect against ptp-A of Candida albicans suggesting ptp-A as the best target to combat P. gingivalis with further in vivo validation.

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Albogami, Sarah, Hadeer Darwish, Hala M. Abdelmigid, Saqer Alotaibi, Ahmed Nour El-Deen, Alaa Alnefaie et Afnan Alattas. « Anticancer Potential of Calli Versus Seedling Extracts Derived from Rosmarinus officinalis and Coleus hybridus ». Current Pharmaceutical Biotechnology 21, n^o 14 (7 décembre 2020) : 1528–38. http://dx.doi.org/10.2174/1389201021666200318114817.

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Background: In Saudi Arabia, the incidence and mortality rates of breast cancer are high. Although current treatments are effective, breast cancer cells develop resistance to these treatments. Numerous studies have demonstrated that active compounds in plant extracts, such as the phenolic compound Rosmarinic Acid (RA), exert anti-cancer effects. Objective: We investigated the anticancer properties of methanolic crude extracts of seedlings and calli of Rosmarinus officinalis and Coleus hybridus, two Lamiaceae species. Methods: MCF-7 human breast cancer cells were treated with methanolic crude extracts obtained from plant calli and seedlings generated in vitro, and cell proliferation was evaluated. Transcriptional profiling of the seedling and callus tissues was also conducted. Results: The mRNA expression levels of RA genes were higher in C. hybridus seedlings than in R. officinalis seedlings, as well as in C. hybridus calli than in R. officinalis calli, except for TAT and C4H. In addition, seedling and callus extracts of both R. officinalis and C. hybridus showed anti-proliferative effects against MCF-7 cells after 24 or 48 h of treatment. Discussion: At a low concentration of 10 μg/mL, C. hybridus calli and seedling extracts showed the most significant anti-proliferative effects after 24 and 48 h of exposure (p < 0.01); controls (doxorubicin) also showed significant inhibition, but lesser than that observed with C. hybridus (p < 0.05). Results with R. officinalis callus and seedling extracts did not significantly differ from those with untreated cells. Conclusion: Methanolic extracts of R. officinalis and C. hybridus are potentially valuable options for breast cancer treatment.

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Kondratyuk, T., et A. Kalinichenko. « Influence of essential oils and polyhexamethyleneguanidine on black yeast-like fungi Exophiala alcalophila ». Bulletin of Taras Shevchenko National University of Kyiv. Series : Biology 68, n^o 3 (2014) : 75–79. http://dx.doi.org/10.17721/1728_2748.2014.68.75-79.

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Influence of essential oils of the following plants Rosmarinum officinalis L., Pinus sibirica Du Tour, Abies sibirica Ledeb., Juniperus communis L., turpentine oil, and polyhexamethyleneguanidine (PHMG) on black yeast-like fungi Exophiala alcalophila FCKU 304 was investigated. Disc-diffusion method was used. Level of the antifungal influence on X. alcalophila found to be different for each chemical. 5% PHMG is characterized by the highest fungicide activity. Level of the antifungal activity of essential oils of plants Rosmarinum officinalis, Pinus sibirica and Abies sibirica is similar to 1.5-3 % PHMG activity. The PHMG and essential oil of Rosmarinum officinalis found to cause exchanges of morphometric indices of cells, colony morphology and intensity of budding E. alcalophila what is considered as adaptations of investigated culture of black yeast-like fungus. Furthermore dimorphic transition 'yeasts – mycelium' of E. alcalophila was observed under influence of essential oil of Rosmarinum officinalis.

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Oalđe Pavlović, Mariana, Stoimir Kolarević, Jelena Đorđević, Jovana Jovanović Marić, Tanja Lunić, Marija Mandić, Margareta Kračun Kolarević et al. « A Study of Phytochemistry, Genoprotective Activity, and Antitumor Effects of Extracts of the Selected Lamiaceae Species ». Plants 10, n^o 11 (27 octobre 2021) : 2306. http://dx.doi.org/10.3390/plants10112306.

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This study was designed to evaluate the genoprotective, antigenotoxic, as well as antitumor potential of methanolic, ethanolic, and aqueous extracts of Melissa officinalis, Mentha × piperita, Ocimum basilicum, Rosmarinus officinalis, Salvia officinalis, and Satureja montana (Lamiaceae), in different model systems. The polyphenols in these extracts were quantified both spectrophotometrically and using HPLC-DAD technique, while DPPH assay was used to assess the antioxidant activity. The genoprotective potential was tested on pUC19 Escherichia coli XL1-blue, and the antigenotoxicity on Salmonella typhimurium TA1535/pSK1002 and human lung fibroblasts, while the antitumor activity was assessed on colorectal cancer cells. Rosmarinic acid, quercetin, rutin, and luteolin-7-O-glucoside were among the identified compounds. Methanolic extracts had the best DPPH-scavenging and SOS-inducing activities, while ethanolic extracts exhibited the highest antigenotoxicity. Additionally, all extracts exhibited genoprotective potential on plasmid DNA. The antitumor effect was mediated by modulation of reactive oxygen species (ROS), nitric oxide (NO) production, and exhibition of genotoxic effects on tumor cells, especially with O. basilicum ethanolic extract. Generally, the investigated extracts were able to provide antioxidant protection for the acellular, prokaryotic, and normal human DNA, while also modulating the production of ROS and NO in tumor cells, leading to genotoxicity toward these cells and their decrease in proliferation.

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Martynov, V. O., O. G. Titov, T. M. Kolombar et V. V. Brygadyrenko. « Influence of essential oils of plants on the migration activity of Tribolium confusum (Coleoptera, Tenebrionidae) ». Biosystems Diversity 27, n^o 2 (6 mai 2019) : 177–85. http://dx.doi.org/10.15421/011924.

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Pest control should be ecologically-based, therefore use of ecologically safe approaches is the best variant. Essential oils of plants can affect the main metabolic, biochemical, physiological and behavioural functions of insects. In the experiment, we evaluated the influence of 20 essential oils on migration activity of imagoes of Tribolium confusum Jacquelin du Val, 1863 in the conditions of a laboratory experiment. Notable repellent activity against T. confusum was exhibited by essential oils of Jasminum officinale and Thuja occidentalis. Essential oils of Zingiber officinale and Cedrus atlantica had an attractant effect on imagoes of T. confusum. Essential oils of Rosmarinus officinalis, Melaleuca alternifolia, Lavandula angustifolia and Cinnamomum verum exhibited repellent properties while essential oils of Juniperus communis and Citrus sinensis had an attractant effect on the pests. Therefore, out of 20 studied essential oils, only four samples had notable biological effect on migration activity of T. confusum imagoes. These data indicate the possibility of using essential oils or their main components as ecologically safe natural repellents against pests of stored wheat and products of its processing.

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Da Silva, Iran Alves, Rozana Firmino de Souza Sultanun, Felipe Stallone Da Silva, Luana Raide Sanguineto Vila, Talismania Da Silva Lira Barbosa, Clêidiane Clemente De Melo, Carolayne Da Silva Laurentino et Jamicelly Rayanna Gomes Da Silva. « POTENCIAL ANTINEOPLÁSICO DO ÁCIDO CARNÓSICO ENCONTRADO NA ESPÉCIE Rosmarinus Officinalis L., LAMIACEAE (ALECRIM) : UMA REVISÃO DE LITERATURA ». Infarma - Ciências Farmacêuticas 34, n^o 1 (8 avril 2022) : 6. http://dx.doi.org/10.14450/2318-9312.v34.e1.a2022.pp6-12.

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O ácido carnósico é um dos principais diterpenos fenólicos antioxidantes presentes no alecrim (Rosmarinus officinalis L.), com destaque no potencial antineoplásico. Assim, este trabalho tem como objetivo investigar na literatura o potencial antineoplásico do ácido carnósico presente em Rosmarinus officinalis L. Trata-se de um estudo retrospectivo narrativo, em que foi realizada uma revisão de literatura nas bases de dados: SciELO, ScienceDirect, PubMed e Google Acadêmico, utilizando as palavraschave: Rosmarinus officinalis, ácido carnósico, antineoplásico e fitoterapia no período de 2015 a 2020. Rosmarinus officinalis L. apresenta diversas ações farmacológicas, dentre elas, antioxidante e antitumoral, conferidos pelos compostos ácido carnósico, carnosol e rosmanol. O efeito antioxidante relacionado a esses compostos fenólicos evita o estresse oxidativo celular e a proliferação celular descontrolada que poderia culminar em um câncer. O ácido carnósico, nesse sentido, é conhecido por exibir atividade anticâncer eficaz contra várias linhas de células tumorais: leucemia e câncer de mama, próstata, pulmão e fígado. É uma molécula capaz de inibir a proliferação e a capacidade de migração em células colorretais humanas por meio da inibição da ativação da uroquinase plasminogênio, enzima esta, responsável pela dissolução dos coágulos de fibrina, incluindo migração celular e inflamação. Nessa revisão foram identificadas as ações quimiopreventivas e antitumorais do ácido carnósico contra melanoma, leucemia, câncer de cólon, pulmão, próstata e fígado, células neoplásicas de neuroblastoma e de glioma.

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Mehalaine, S., O. Belfadel, T. Menasria et A. Messaili. « Chemical Composition and Antibacterial Activity of Essential Oils of Three Medicinal Plants from Algerian Semi-Arid Climatic Zone ». Phytothérapie 16, S1 (décembre 2018) : S155—S163. http://dx.doi.org/10.3166/phyto-2019-0150.

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The present study was carried out to determine, for the first time, the chemical composition and antibacterial activity of essential oils derived from the aerial parts of three aromatic plants Thymus algeriensis Boiss & Reut, Rosmarinus officinalis L., and Salvia officinalis L. growing under semiarid conditions. The essential oils were chemically analyzed and identified by gas chromatography (GC) and GC/ mass spectrometry (GC/MS) and their antimicrobial activity was individually evaluated against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa using both agar disk diffusion and agar dilution methods. The major constituents of Thymus algeriensis essential oil were identified as camphor (13.62%), 1,8-cineol (6.00%), borneol (5.74%), viridiflorol (4.00%), and linalool (3.93%). For Rosmarinus officinalis essential oil, 48 compounds were characterized, of which the main constituents were camphor (17.09%), Z-β-ocimene (10.88%), isoborneol (9.68%), α-bisabolol (7.89%), and borneol (5.11%). While, Salvia officinalis essential oil was characterized by β-thujone (16.44%), followed by viridiflorol (10.93%), camphor (8.99%), 1,8-cineol (8.11%), trans-caryophyllene (5.85%), and α-humulene (4.69%) as the major components. Notably, results from antibacterial screening indicated that Thymus algeriensis and Salvia officinalis essential oils exhibited a strong inhibitory effect against both Escherichia coli and Staphylococcus aureus compared to Rosmarinus officinalis essential oil. Further, less activity was recorded against Pseudomonas aeruginosa for the three tested essential oils.

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Pimentel, Adelma do Socorro Gonçalves. « Rosmarinus Officinalis L. para o tratamento da ansiedade : uma revisão de literatura ». Research, Society and Development 10, n^o 11 (1 septembre 2021) : e285101119635. http://dx.doi.org/10.33448/rsd-v10i11.19635.

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A ansiedade comum e as formas psicopatológicas são tratadas incluindo remédios alopáticos, o que pode ocasionar dependência do uso de medicamentos. Assim, neste texto apresentamos o enfoque fenomenológico em psicopatologia e a contribuição do Rosmarinus Officinalis no tratamento integral da ansiedade, por meio de uma revisão sistemática de literatura descrevendo pesquisas que incluem o Rosmarinus Officinalis no tratamento da ansiedade nas formas de tintura, chá, e óleo essencial como moderador conjunto a redução do cansaço físico e mental. Os procedimentos envolvem coleta nas bases de dados nacionais, período de 2010 a 2020. Destaca-se entre os resultados que os estudos clínicos e observacionais do Rosmarinus Officinalis são mais realizados com modelos animais; contudo demonstram potencial terapêutico no tratamento da ansiedade. Considera-se que agregar o mesmo em projetos psicoterapêuticos sistemáticos de base fenomenológica existencial favorece a melhora da concentração pessoal, a respiração e a delimitação do sentido que a ansiedade tem na vida emocional e social das pessoas.

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Nadeem, Muhammad, Muhammad Imran, Tanweer Aslam Gondal, Ali Imran, Muhammad Shahbaz, Rai Muhammad Amir, Muhammad Wasim Sajid et al. « Therapeutic Potential of Rosmarinic Acid : A Comprehensive Review ». Applied Sciences 9, n^o 15 (2 août 2019) : 3139. http://dx.doi.org/10.3390/app9153139.

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Naturally occurring food-derived active ingredients have received huge attention for their chemopreventive and chemotherapy capabilities in several diseases. Rosmarinic acid (RA) is a caffeic acid ester and a naturally-occurring phenolic compound in a number of plants belonging to the Lamiaceae family, such as Rosmarinus officinalis (rosemary) from which it was formerly isolated. RA intervenes in carcinogenesis through different ways, including in tumor cell proliferation, apoptosis, metastasis, and inflammation. On the other hand, it also exerts powerful antimicrobial, anti-inflammatory, antioxidant and even antidepressant, anti-aging effects. The present review aims to provide an overview on anticancer activities of RA and to deliberate its therapeutic potential against a wide variety of diseases. Given the current evidence, RA may be considered as part of the daily diet in the treatment of several diseases, with pre-determined doses avoiding cytotoxicity.

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