Littérature scientifique sur le sujet « RoGFP2 »
Créez une référence correcte selon les styles APA, MLA, Chicago, Harvard et plusieurs autres
Sommaire
Consultez les listes thématiques d’articles de revues, de livres, de thèses, de rapports de conférences et d’autres sources académiques sur le sujet « RoGFP2 ».
À côté de chaque source dans la liste de références il y a un bouton « Ajouter à la bibliographie ». Cliquez sur ce bouton, et nous générerons automatiquement la référence bibliographique pour la source choisie selon votre style de citation préféré : APA, MLA, Harvard, Vancouver, Chicago, etc.
Vous pouvez aussi télécharger le texte intégral de la publication scolaire au format pdf et consulter son résumé en ligne lorsque ces informations sont inclues dans les métadonnées.
Articles de revues sur le sujet "RoGFP2"
Albrecht, Simone C., Mirko C. Sobotta, Daniela Bausewein, Isabel Aller, Rüdiger Hell, Tobias P. Dick et Andreas J. Meyer. « Redesign of Genetically Encoded Biosensors for Monitoring Mitochondrial Redox Status in a Broad Range of Model Eukaryotes ». Journal of Biomolecular Screening 19, no 3 (16 août 2013) : 379–86. http://dx.doi.org/10.1177/1087057113499634.
Texte intégralLiu, Ting-Hang, Mohammad A. Yaghmour, Miin-Huey Lee, Thomas M. Gradziel, Johan H. J. Leveau et Richard M. Bostock. « An roGFP2-Based Bacterial Bioreporter for Redox Sensing of Plant Surfaces ». Phytopathology® 110, no 2 (février 2020) : 297–308. http://dx.doi.org/10.1094/phyto-07-19-0237-r.
Texte intégralXu, Xiuling, Katharina von Löhneysen, Katrin Soldau, Deborah Noack, Andrew Vu et Jeffrey S. Friedman. « A novel approach for in vivo measurement of mouse red cell redox status ». Blood 118, no 13 (29 septembre 2011) : 3694–97. http://dx.doi.org/10.1182/blood-2011-03-342113.
Texte intégralXu, Xiuling, Katharina von Loehneysen, Deborah Noack, Andrew Vu et Jeff S. Friedman. « A Novel Approach for In Vivo Measurement of Red Cell Redox Status ». Blood 116, no 21 (19 novembre 2010) : 2036. http://dx.doi.org/10.1182/blood.v116.21.2036.2036.
Texte intégralde Cubas, Laura, Valeriy V. Pak, Vsevolod V. Belousov, José Ayté et Elena Hidalgo. « The Mitochondria-to-Cytosol H2O2 Gradient Is Caused by Peroxiredoxin-Dependent Cytosolic Scavenging ». Antioxidants 10, no 5 (6 mai 2021) : 731. http://dx.doi.org/10.3390/antiox10050731.
Texte intégralXu, Xiuling, et Jeff S. Friedman. « In Vivo Monitoring of Red Cell Redox Status to Screen for Potential Hematotoxicity of Anti-Malarial Drugs ». Blood 118, no 21 (18 novembre 2011) : 2099. http://dx.doi.org/10.1182/blood.v118.21.2099.2099.
Texte intégralFernández-Puente, Escarlata, et Jesús Palomero. « Genetically Encoded Biosensors to Monitor Intracellular Reactive Oxygen and Nitrogen Species and Glutathione Redox Potential in Skeletal Muscle Cells ». International Journal of Molecular Sciences 22, no 19 (8 octobre 2021) : 10876. http://dx.doi.org/10.3390/ijms221910876.
Texte intégralGarcía-Quirós, Estefanía, Juan de Dios Alché, Barbara Karpinska et Christine H. Foyer. « Glutathione redox state plays a key role in flower development and pollen vigour ». Journal of Experimental Botany 71, no 2 (26 septembre 2019) : 730–41. http://dx.doi.org/10.1093/jxb/erz376.
Texte intégralMorgan, Bruce, Mirko C. Sobotta et Tobias P. Dick. « Measuring EGSH and H2O2 with roGFP2-based redox probes ». Free Radical Biology and Medicine 51, no 11 (décembre 2011) : 1943–51. http://dx.doi.org/10.1016/j.freeradbiomed.2011.08.035.
Texte intégralCosta, Cláudio F., Celien Lismont, Serhii Chornyi, Hongli Li, Mohamed A. F. Hussein, Hans R. Waterham et Marc Fransen. « Functional Analysis of GSTK1 in Peroxisomal Redox Homeostasis in HEK-293 Cells ». Antioxidants 12, no 6 (7 juin 2023) : 1236. http://dx.doi.org/10.3390/antiox12061236.
Texte intégralThèses sur le sujet "RoGFP2"
Dehaene, Noémie. « Functional analysis of a cytoplasmic male sterility in Arabidopsis thaliana ». Thesis, Université Paris-Saclay (ComUE), 2017. http://www.theses.fr/2017SACLS418/document.
Texte intégralThis work aims at better understand the events leading to pollen abortion in a recently discovered gametophytic cytoplasmic male sterility (CMS) in Arabidopsis thaliana. Although CMS have been widely used in hybrid seed production in many crops, the physiological mechanisms leading to pollen death by the mitochondrial sterilizing genes in the permissive (maintainer) nuclear backgrounds are poorly understood. Association genetics previously identified orf117Sha as a candidate mitochondrial CMS-associated gene.In a first part, I analyzed the expression of the orf117Sha gene in sterile plants and in fertile plants carrying nuclear genes restoring male fertility. I observed unusual features of its mRNA, but detected no difference at this level between sterile and restored plants. Oppositely, the ORF117SHA protein seems to be accumulated specifically in the sterile line, supporting its role in CMS. A phenocopy attempt by transgenesis suggested a possible link between a female and male gametophytic lethality and the ORF117SHA, even though few individuals could be analyzed.In a second part, I observed pollen development in sterile plants and fertile controls using different cytological approaches. My results show a progressive pollen death starting from the binucleate stage in the sterile. Prior to abortion, pollen mitochondria swell before rupture, and the development stops. I used confocal microscopy combined with genetically encoded sensors to explore specific physiological features in pollen and vegetative tissues of sterile plants. With ATeam, which allows the assessment of ATP content in the cytosol, I could challenge the generally accepted hypothesis of an ATP deficiency leading to pollen abortion in CMS. Indeed, the ATP production does not seem to be affected in the sterile line. With a mitochondria-addressed roGFP2-Grx1, I was able to assess the redox state of the glutathione pool in vegetative tissues and in the male gametophyte. I observed an overoxydation of the glutathione pool in mitochondria of the sterile line, in vegetative tissue investigated and in the pollen grain. This overoxydation seems to be linked to the CMS as it is annihilated by the presence of restorer genes.My results pave the way for further exploration of the links between the sterility protein, mitochondrial morphology changes, mitochondrial overoxydation, and pollen development arrest and death in the A. thaliana CMS
Caubrière, Damien. « Développement de nouveaux biosenseurs redox pour composés soufrés ». Electronic Thesis or Diss., Université de Lorraine, 2022. http://www.theses.fr/2022LORR0359.
Texte intégralOver the last decade, the development of fluorescent redox biosensors has provided tools to study the in vivo dynamics of molecules such as the reduced and oxidized forms of glutathione or hydrogen peroxide. Cysteine being a key metabolite of sulfur metabolism, this PhD project aimed at developing a fluorescent redox biosensor specific for cysteine by coupling an oxidoreductase to roGFP2 (reduction-oxidation green fluorescent protein). First, the activities of several isoforms of cysteine desulfurases (CD) and rhodanese-domain containing proteins (Rhd), catalyzing cysteine desulfuration and trans-persufidation reactions, respectively, were analyzed in vitro in order to determine whether they could constitute good candidates for this oxidoreductase activity. These analyses revealed that a natural chimeric protein possessing both CD and Rhd domains efficiently oxidizes roGFP2, by catalyzing trans-persulfidation reactions from cysteine to roGFP2. This candidate protein was then fused to roGFP2 to generate the CD-Rhd-roGFP2 biosensor. In vitro, this protein is sensitive to oxidation in the presence of physiological concentrations of cysteine whereas oxidation by thiosulfate, another potential substrate of the Rhd domain, is negligible. In addition, the trans-persulfidation reactions between the protein domains leading to the oxidation of roGFP2 are not inhibited by physiological reducing systems. Nevertheless, the glutathione/glutaredoxin system specifically reduces roGFP2. The expression of this biosensor in the bacterium Escherichia coli revealed a dynamic response of the biosensor to exogenous addition of cysteine or cystine, paving the way for similar studies in organelles from other eukaryotic model organisms
Schneider, Jannis Frederic [Verfasser], Lars I. [Gutachter] Leichert et Joachim [Gutachter] Rassow. « Untersuchung des Redoxzustandes des Periplasmas und Aufklärung früher redox-regulatorischer Ereignisse in Escherichia coli während der eukaryotischen Immunantwort unter Verwendung von roGFP2 basierten Sonden / Jannis Frederic Schneider ; Gutachter : Lars I. Leichert, Joachim Rassow ; Medizinische Fakultät ». Bochum : Ruhr-Universität Bochum, 2021. http://d-nb.info/1235224279/34.
Texte intégralMaciejuk, Anna-Maria. « Measurements of redox potential during apoptosis ». Thesis, University of Edinburgh, 2017. http://hdl.handle.net/1842/28862.
Texte intégralEdwards, Sarah. « Disulfide-Mediated Modifications of roGFP and their Impact on Its Use as a Redox Sensor ». Thesis, The University of Arizona, 2011. http://hdl.handle.net/10150/144316.
Texte intégralWagener, Kerstin Charlotte [Verfasser], Michael [Akademischer Betreuer] Müller, Michael [Gutachter] Müller et Stefan [Gutachter] Jakobs. « Transgene Redoxindikator-Mäuse mit mitochondrialer roGFP1-Expression : Phänotypisierung, neuronales Verteilungsmuster und Sensorfunktionalität / Kerstin Charlotte Wagener ; Gutachter : Michael Müller, Stefan Jakobs ; Betreuer : Michael Müller ». Göttingen : Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2017. http://d-nb.info/1149956364/34.
Texte intégralSouza, Arnaldo Henrique de. « Modulação redox, função e sobrevivência de células β-pancreáticas : evidência sobre o papel da enzima NADPH oxidase-2 (NOX2) em um modelo in vitro de glicotoxicidade ». Universidade de São Paulo, 2016. http://www.teses.usp.br/teses/disponiveis/42/42137/tde-06092016-094234/.
Texte intégralOxidative stress and NADPH oxidase-2 (NOX2) enzyme are associated to the decline of the functional β-cell mass in type 2 diabetes (T2D). Here, we tested the role of NOX2 on β-cell glucotoxicity. NOX2 knockout (NOX2 KO) and wild type (WT) C57BL/6J mice islets were isolated and cultured up to 3 weeks at 10 or 30 mmol/l glucose concentrations (G10 and G30, respectively). The insulin secretion was higher in NOX2-KO vs. WT islets despite similar metabolic and cytosolic glutathione-redox potential (EGSH) changes. The prolonged culture at G30 increases the H2O2 concentration and cytosolic thiol oxidation, followed by increased βcell apoptosis but preserving maximal secretory response. These responses were almost identical in both types of islets. In conclusion, NOX2 is a negative regulator of insulin secretion in C57BL/6J mouse islets, but is not a critical component for β-cell survival in a model of glucotoxicity in vitro.
Kolbrink, Benedikt [Verfasser], Michael [Akademischer Betreuer] Müller et Jochen [Akademischer Betreuer] Staiger. « Charakterisierung eines transgenen Mausmodells mit spezifischer zytosolischer Expression des optischen Redox-Indikators roGFP1 in Neuronen / Benedikt Kolbrink. Gutachter : Michael Müller ; Jochen Staiger. Betreuer : Michael Müller ». Göttingen : Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2015. http://d-nb.info/1077096410/34.
Texte intégralKizina, Kathrin Michaela. « Funktionelles ROS/Redox Imaging, basierend auf genetisch-kodierten optischen Sensoren, exzitationsratiometrischer Zwei-Photonen-Mikroskopie und Fluoreszenzlebenszeiten ». Doctoral thesis, 2019. http://hdl.handle.net/21.11130/00-1735-0000-0003-C139-3.
Texte intégralWagener, Kerstin Charlotte. « Transgene Redoxindikator-Mäuse mit mitochondrialer roGFP1-Expression : Phänotypisierung, neuronales Verteilungsmuster und Sensorfunktionalität ». Doctoral thesis, 2017. http://hdl.handle.net/11858/00-1735-0000-0023-3F99-F.
Texte intégralChapitres de livres sur le sujet "RoGFP2"
Ugalde, José Manuel, Lara Fecker, Markus Schwarzländer, Stefanie J. Müller-Schüssele et Andreas J. Meyer. « Live Monitoring of ROS-Induced Cytosolic Redox Changes with roGFP2-Based Sensors in Plants ». Dans Methods in Molecular Biology, 65–85. New York, NY : Springer US, 2022. http://dx.doi.org/10.1007/978-1-0716-2469-2_5.
Texte intégralLismont, Celien, Paul A. Walton et Marc Fransen. « Quantitative Monitoring of Subcellular Redox Dynamics in Living Mammalian Cells Using RoGFP2-Based Probes ». Dans Methods in Molecular Biology, 151–64. New York, NY : Springer New York, 2017. http://dx.doi.org/10.1007/978-1-4939-6937-1_14.
Texte intégralBuratti, Stefano, Matteo Grenzi, Giorgia Tortora, Sara Paola Nastasi, Elisa Dell’Aglio, Andrea Bassi et Alex Costa. « Noninvasive In Planta Live Measurements of H2O2 and Glutathione Redox Potential with Fluorescent roGFPs-Based Sensors ». Dans ROS Signaling in Plants, 45–64. New York, NY : Springer US, 2024. http://dx.doi.org/10.1007/978-1-0716-3826-2_4.
Texte intégralActes de conférences sur le sujet "RoGFP2"
Niculescu, Mihai Alexandru, Stefan Ruseti et Mihai Dascalu. « RoGPT2 : Romanian GPT2 for Text Generation ». Dans 2021 IEEE 33rd International Conference on Tools with Artificial Intelligence (ICTAI). IEEE, 2021. http://dx.doi.org/10.1109/ictai52525.2021.00183.
Texte intégralWierer, S., K. Elgass, S. Bieker, U. Zentgraf, A. J. Meixner et F. Schleifenbaum. « Determination of the in vivo redox potential using roGFP and fluorescence spectra obtained from one-wavelength excitation ». Dans SPIE BiOS, sous la direction de Daniel L. Farkas, Dan V. Nicolau et Robert C. Leif. SPIE, 2011. http://dx.doi.org/10.1117/12.873753.
Texte intégralRapports d'organisations sur le sujet "RoGFP2"
Friedman, Haya, Chris Watkins, Susan Lurie et Susheng Gan. Dark-induced Reactive Oxygen Species Accumulation and Inhibition by Gibberellins : Towards Inhibition of Postharvest Senescence. United States Department of Agriculture, décembre 2009. http://dx.doi.org/10.32747/2009.7613883.bard.
Texte intégral