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Littérature scientifique sur le sujet « Rhizobium leguminosarum Molecular aspects »

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Publié le 4 juin 2021

Mis à jour le 29 janvier 2023

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Articles de revues sur le sujet "Rhizobium leguminosarum Molecular aspects"

Chalifour, François-P., et Nicole Benhamou. « Indirect evidence for cellulase production by Rhizobium in pea root nodules during bacteroid differentiation : cytochemical aspects of cellulose breakdown in rhizobial droplets ». Canadian Journal of Microbiology 35, n^o 9 (1 septembre 1989) : 821–29. http://dx.doi.org/10.1139/m89-138.

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Cytochemical localization of cellulosic β-(1–4) glucans in pea (Pisum sativum L.) nodules at different stages of infection by an effective isolate of Rhizobium leguminosarum biovar viceae was studied using a gold-complexed exoglucanase. Cellulose subunits were present in great amounts in root cell walls, as shown by intense and regular labeling by gold particles. Labeling was unevenly distributed over the thin walls of emerging infection threads. In more developed infection threads, labeling was more intense and evenly distributed than in emerging threads, although slightly altered, unlabeled wall areas were frequently observed at the growing tips. Droplets containing rhizobia, which originated from infection threads, were surrounded by labeled wall-like material. Rhizobial droplets were either single- or multi-celled, and were sometimes separated by inner, unevenly labeled compartments. The surrounding wall-like material was irregularly labeled, and unlabeled wall areas, neighbouring intensely labeled ones, were observed frequently. There was an absence of labeling ahead of the rhizobia that escaped from the droplets, but degenerating wall-like material was present around the escaping rhizobia, mainly on their sides. At more advanced stages of development, labeling was present only over the outermost wall layers of rhizobial droplets, indicating that inner portions were degraded first. These observations suggest that a hydrolytic enzyme is involved in the sequence of events from infection thread formation through rhizobial release in the host cell cytoplasm, and that the hydrolytic enzyme is of rhizobial origin.Key words: Rhizobium–Pisum symbiosis, root nodules, rhizobial droplets, cellulose, colloidal gold.

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Russo, Daniela M., Alan Williams, Anne Edwards, Diana M. Posadas, Christine Finnie, Marcelo Dankert, J. Allan Downie et Angeles Zorreguieta. « Proteins Exported via the PrsD-PrsE Type I Secretion System and the Acidic Exopolysaccharide Are Involved in Biofilm Formation by Rhizobium leguminosarum ». Journal of Bacteriology 188, n^o 12 (15 juin 2006) : 4474–86. http://dx.doi.org/10.1128/jb.00246-06.

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ABSTRACT The type I protein secretion system of Rhizobium leguminosarum bv. viciae encoded by the prsD and prsE genes is responsible for secretion of the exopolysaccharide (EPS)-glycanases PlyA and PlyB. The formation of a ring of biofilm on the surface of the glass in shaken cultures by both the prsD and prsE secretion mutants was greatly affected. Confocal laser scanning microscopy analysis of green-fluorescent-protein-labeled bacteria showed that during growth in minimal medium, R. leguminosarum wild type developed microcolonies, which progress to a characteristic three-dimensional biofilm structure. However, the prsD and prsE secretion mutants were able to form only an immature biofilm structure. A mutant disrupted in the EPS-glycanase plyB gene showed altered timing of biofilm formation, and its structure was atypical. A mutation in an essential gene for EPS synthesis (pssA) or deletion of several other pss genes involved in EPS synthesis completely abolished the ability of R. leguminosarum to develop a biofilm. Extracellular complementation studies of mixed bacterial cultures confirmed the role of the EPS and the modulation of the biofilm structure by the PrsD-PrsE secreted proteins. Protein analysis identified several additional proteins secreted by the PrsD-PrsE secretion system, and N-terminal sequencing revealed peptides homologous to the N termini of proteins from the Rap family (Rhizobium adhering proteins), which could have roles in cellular adhesion in R. leguminosarum. We propose a model for R. leguminosarum in which synthesis of the EPS leads the formation of a biofilm and several PrsD-PrsE secreted proteins are involved in different aspects of biofilm maturation, such as modulation of the EPS length or mediating attachment between bacteria.

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Figueira, Etelvina Maria de Almeida Paula, Ana Isabel Gusmão Lima et Sofia Isabel Almeida Pereira. « Cadmium tolerance plasticity in Rhizobium leguminosarum bv. viciae : glutathione as a detoxifying agent ». Canadian Journal of Microbiology 51, n^o 1 (1 janvier 2005) : 7–14. http://dx.doi.org/10.1139/w04-101.

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Rhizobium leguminosarum bv. viciae strains expressing different degrees of tolerance to metal stress were used in this work to study the basic mechanisms underlying heavy metal tolerance. We used various parameters to evaluate this response. The strains' growth responses under different Cd2+ concentrations were determined and we reported variation in Cd2+ tolerance. Total soluble protein content decreased drastically, revealing the toxic effects that intracellular Cd2+ imposes on cellular metabolism, but this decrease in protein content was particularly evident in sensitive and moderately tolerant strains. Tolerant strains presented the highest intracellular and wall-bound Cd2+ concentrations. Cd2+ induced increases in the expression of some specific proteins, which were identical in all tolerant strains. Glutathione levels remained unaltered in the sensitive strain and increased significantly in tolerant and moderately tolerant strains, suggesting the importance of glutathione in coping with metal stress. This work suggests that efflux mechanisms may not be the only system responsible for dealing with heavy metal tolerance. A clear correlation between glutathione levels and Cd2+ tolerance is reported, thus adding a novel aspect in bacteria protection against heavy metal deleterious effects.Key words: glutathione, heavy metal, protein expression, rhizobia, thiol quantification.

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Shahzad, Farood, Muhammad Kamran Taj, Ferhat Abbas, Muhammad Shafee, Safed Ahmed Essote, Imran Taj et Abdul Manan Achakzai. « Microbiological studies on Rhizobium leguminosarum isolated from pea (Pisum sativum L.) ». Bangladesh Journal of Botany 48, n^o 4 (31 décembre 2019) : 1223–29. http://dx.doi.org/10.3329/bjb.v48i4.49079.

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Rhizobia are the true bacteria that establish symbiotic relationship leading to the development of new root nodules. This study has been designed to evaluate the microbiological aspects of Rhizobium leguminosarum in target area. A total of 1000 (200 from each site) roots were collected from five different agriculture fields (Quetta, Pishin, Killa Abdulla, Kuchlak and Hanna Urak) and screened through different standard microbiological procedures. Results revealed that 665/1000 (66.5%) roots samples were positive for Rhizobium leguminosarum. The highest percentage was from Pishin 180/200 (18%) and Killa Abdullah 160/200 (16%). A remarkable growth of Rhizobium leguminosarum was noted at 28 to 30°C whereas, less growth was recorded at 24, 34 and 42°C. Similarly, Rhizobium leguminosarum showed growth at pH 5 to 10, but superlative pH values for the growth of Rhizobium leguminosarum were from 6 to 8 pH. The PCR reconfirmed 1300 bp band of 16S rRNA gene of Rhizobium leguminosarum. The organism was further applied as biofertilizer and showed promising results in subjected plants. Medicinal plants application showed that Rhizobium leguminosarum was sensitive to different plants. However, the effects of insecticides showed that Cypermethrin exhibited least zone of inhibition 10 and 11 mm, while Chlorpyrifos showed least zone of inhibition 14 and 17 mm by using disc and well method with (1: 16) dilution. These findings ensure the devastation of microbiota in rhizosphere with rational use of these pesticides that may result in adverse effects over crop productions in the region.

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Enibukun, Jesupemi Mercy, et Bolatito Esther Boboye. « Molecular characterization and evaluation of crude oil remediation potential of some rhizobia isolated from plant root nodules ». Nova Biotechnologica et chimica 19, n^o 1 (30 juin 2020) : 80–88. http://dx.doi.org/10.36547/nbc.v19i1.580.

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This study aimed to determine the molecular identities and genetic relatedness of rhizobia isolated from pigeon pea and pinto beans, and assess their remediation potential in the presence of 1 %, 3 % and 5 % (w/v) crude oil in minimal medium for 7 days incubation period. Standard microbiological and molecular methods which include amplification and purification of 16S rRNA, agarose gel electrophoresis, and sequencing. Results showed molecular identities of six rhizobia from pigeon peas as Bradyrhizobium diazoefficiens USDA122, Rhizobium leguminosarum WSM2304, Bradyrhizobium japonicum N61, Rhizobium leguminosarum N741, Rhizobium leguminosarum BIHIB1217, and Bradyrhizobium japonicum E109; and three rhizobia obtained from pinto beans were Rhizobium leguminosarum N871, Bradyrhizobium diazoefficiens USDA110 and Bradyrhizobium japonicum SEMIA5079. All tested rhizobia (9) showed petroleum degradation ability, as they all grew in the 1, 3 and 5 % (w/v) crude oil minimal medium under laboratory conditions. B. diazoefficiens USDA122 showed the highest optical density (OD) value of 1.184 ± 0.05 on 7th day at 1 % (w/v) crude oil contamination, while R. leguminosarum N741 has the lowest OD value of 0.372 ± 0.02 at 5 % (w/v) crude oil on 7th day. For all the rhizobia, increase occurred throughout incubation period at 1, 3 and 5 % (w/v) except Rhizobium leguminosarum N741 and R. leguminosarum BIHIB1217. In conclusion, the association of R. leguminosarum BIHIB1217 and R. leguminosarum N871 from pigeon pea and pinto beans respectively, were found most effective in crude oil degradation and thus they are recommended as a promising association for remediation of crude oil spilled soils.

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Soberón-Chávez, Gloria, et Rebeca Nájera. « Isolation from soil of Rhizobium leguminosarum lacking symbiotic information ». Canadian Journal of Microbiology 35, n^o 4 (1 avril 1989) : 464–68. http://dx.doi.org/10.1139/m89-071.

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Bacteria resembling Rhizobium leguminosarum, but lacking symbiotic information, were isolated from soil of two different geographical origins. One of these bacteria belongs to a previously described Rhizobium leguminosarum bv. phaseoli somatic serogroup, is fully complemented for nodulation and nitrogen fixation by an R. leguminosarum bv. phaseoli symbiotic plasmid, and is able to compete for bean nodulation with indigenous R. leguminosarum bv. phaseoli strains. This is the first report giving evidence for persistence in soil of Rhizobium lacking symbiotic information.Key words: Rhizobium ecology, symbiotic plasmid, nodulation, plasmid transfer.

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Laguerre, Gisèle, Marc Bardin et Noëlle Amarger. « Isolation from soil of symbiotic and nonsymbiotic Rhizobium leguminosarum by DNA hybridization ». Canadian Journal of Microbiology 39, n^o 12 (1 décembre 1993) : 1142–49. http://dx.doi.org/10.1139/m93-172.

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A procedure based upon DNA hybridization was developed for the specific detection of Rhizobium leguminosarum and its different biovars among bacteria isolated from soil. DNA colony hybridization and restriction fragment length polymorphism analysis with a R. leguminosarum chromosomal probe were found to be species specific for R. leguminosarum and Rhizobium etli. By using R. leguminosarum nod gene probes, biovar specificity was obtained. Of 302 soil isolates screened for their inability to grow on Luria-Bertani agar medium, 13 strains could be assigned to the R. leguminosarum species on the basis of DNA homology to the chromosomal probe and antibiotic resistance tests. Of these strains, three and two were assigned by colony hybridization and subsequent plant host specificity tests, respectively, to R. leguminosarum biovars viciae and trifolii. The eight other R. leguminosarum soil isolates lacked symbiotic information but were able to gain nodulation capacity with the acquisition of a conjugative symbiotic plasmid. They were thus considered as nonsymbiotic R. leguminosarum.Key words: Rhizobium leguminosarum, DNA hybridization, soil, symbiotic genes.

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Rioux, Clément R., D. Carlyle Jordan et James B. M. Rattray. « Anthranilate-promoted iron uptake in Rhizobium leguminosarum ». Archives of Biochemistry and Biophysics 248, n^o 1 (juillet 1986) : 183–89. http://dx.doi.org/10.1016/0003-9861(86)90415-7.

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Velázquez, Encarna, Esperanza Martı́nez-Romero, Dulce Nombre Rodrı́guez-Navarro, Martha E. Trujillo, Antonio Daza, Pedro F. Mateos, Eustoquio Martı́nez-Molina et Peter van Berkum. « Characterization of Rhizobial Isolates of Phaseolus vulgaris by Staircase Electrophoresis of Low-Molecular-Weight RNA ». Applied and Environmental Microbiology 67, n^o 2 (1 février 2001) : 1008–10. http://dx.doi.org/10.1128/aem.67.2.1008-1010.2001.

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ABSTRACT Low-molecular-weight (LMW) RNA molecules were analyzed to characterize rhizobial isolates that nodulate the common bean growing in Spain. Since LMW RNA profiles, determined by staircase electrophoresis, varied across the rhizobial species nodulating beans, we demonstrated that bean isolates recovered from Spanish soils presumptively could be characterized as Rhizobium etli,Rhizobium gallicum, Rhizobium giardinii,Rhizobium leguminosarum bv. viciae and bv. trifolii, andSinorhizobium fredii.

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Kucey, R. M. N., et M. F. Hynes. « Populations of Rhizobium leguminosarum biovars phaseoli and viceae in fields after bean or pea in rotation with nonlegumes ». Canadian Journal of Microbiology 35, n^o 6 (1 juin 1989) : 661–67. http://dx.doi.org/10.1139/m89-107.

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Populations of Rhizobium leguminosarum bv. phaesoli and bv. viceae in southern Alberta soils were measured over a period of 4 years using a most probable number method. Five fields cropped to bean (Phaseolus vulgaris L.), five fields cropped to pea (Pisum sativum L.), and two fields cropped to wheat were used as test sites. Legume crops had received appropriate legume inoculants. Fields were sampled in the fall of the crop year and in the spring of the following 3 years during which fields were cropped to nonlegumes or left fallow. Numbers of R. leguminosarum bv. phaseoli were 100 to 1000 times higher in fields that had been planted to bean than in fields that had been planted to pea or wheat. Fields that had been planted to pea maintained populations of R. leguminosarum bv. viceae 10 to 100 times higher than fields that had been planted to bean or wheat. Wheat fields, which had never had legumes grown in them, contained between 1 and 100 rhizobia per gram of soil of both biovars of R. leguminosarum, indicating that both biovars are native to southern Alberta soils. The numbers of rhizobia did not decrease in proportion to the population of other bacteria in the soil over the duration of the experiment. Plasmid profiles of soil Rhizobium isolates obtained in the last year of the experiment showed that none of the isolates had plasmid profiles similar to those of strains added as inoculants in the 1st year of the experiment. These results show that fields cropped to legumes and receiving rhizobial inoculants in this study maintained high populations of rhizobia for several years after harvest of the legume crop.Key words: Rhizobium leguminosarum bv. phaseoli, Rhizobium leguminosarum bv. viceae, nodule, plasmid profiles, inoculum potential, rhizobium competition.

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Thèses sur le sujet "Rhizobium leguminosarum Molecular aspects"

Heinrich, Keith. « Ecological and molecular studies on rhizobial rhizopines ». Title page, contents and summary only, 1999. http://web4.library.adelaide.edu.au/theses/09PH/09phh469.pdf.

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Corrigendum attached to back cover. Includes bibliographical references (leaves 160-190). Investigates the role of rhizopines in rhizobial competition for nodulation, and to isolate the rhizopine synthesis genes in Rhizobium leguminosarum bv. viciae.

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Bahar, Masoud. « Molecular biology of rhizopine genes in Rhizobium leguminosarum br. viciae / ». Title page, table of contents and summary only, 1997. http://web4.library.adelaide.edu.au/theses/09PH/09phb1508.pdf.

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Simpkins, Sean A. « The DnaK molecular chaperone of Rhizobium leguminosarum ». Thesis, University of East Anglia, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.302035.

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Stevens, James B. « The molecular genetics of iron uptake in rhizobium leguminosarum ». Thesis, University of East Anglia, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.323075.

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Rossen, L. « Molecular analysis of the nodulation genes of Rhizobium leguminosarum ». Thesis, University of East Anglia, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.370396.

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Sindhu, Satyavir Singh. « Molecular analysis of lipopolysaccharide and membrane associated proteins in Rhizobium leguminosarum ». Thesis, University of East Anglia, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.256988.

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This study describes the use of monoclonal antibodies to investigate molecular components of the Rhizobium cell surface that might be important for symbiotic interactions with the host legume. Components that have been identified include lipopolysaccharide and both membrane-associated and secreted proteins. Differences were observed in the structure and antigenicity of lipopolysaccharide (LPS) from free-living rhizobia compared with that of endosymbiotic bacteroids. Culture pH, oxygen concentration and carbon source were all found to be important factors that could affect the expression of LPS antigens within the nodule. Mutants with altered LPS demonstrated that complete LPS structures are necessary for effective symbiosis. Monoclonal antibody nM 25 identified a protease-sensitive epitope that appeared to be attached to a particular species of LPS macromolecule (identified by MAC 114 antibody). Immunocytochemical localization studies of pea nodule sections revealed that JIM 25 antigen, present on the cell surface, was expressed in infection threads but its expression was low in the symbiotic zone containing mature bacteroids. A 38kDa secreted protein was identified by JIM 24 antibody. Fractionation of nodule extracts by differential centrifugation suggested that the protein was present in the peribacteroid space. A 55kDa membrane protein recognized by MAC 115 antibody proved to be a species-specific marker for R. leguminosarum. The structural gene(s) for this protein are encoded on a 2.8kb EcoRI fragment. Localised mutagenesis of this DNA region with the transposon TnPhoA (which carries a promoter-less gene for alkaline phosphatase) provided evidence that the 55kDa protein was' membraneassociated. However, attempts to "marker-exchange" the transposon-induced mutations from cosmid DNA into the Rhizobium genome were unsuccessful, suggesting that the 55kDa protein is essential for growth of free-living rhizobia. In a DNA region adjoining this 2.8kb EcoRI fragment that encodes the 55kDa protein, a new gene has been described, termed muc. When present on a cosmid the muc gene from R. leguminosarum conferred non-mucoid colony morphology on R. meliloti strain B287. "Marker-exchange" of muc::Tn5 mutations from the plasmid to R. ieguminosarum 8002 (bv. phaseo/i) resulted in derivatives that had lost the ability to nodulate Phaseolus beans. However, marker-exchange into R. leguminosarum B556 (bv. viciae) resulted in mutants that showed no abnormal symbiotic phenotypes on peas. The cosmid carrying the muc and 55kDa protein determinants (pIJ1639) was subjected to saturation transposon mutagenesis with TnPhoA This study revealed several new genes that probably encode membrane-associated or secreted proteins. In some cases gene transcription was dependent on the presence of hesperitin [which is known to be an activator of Rhizobium nodulation (nod) genes]. A 4.6kb EcoRI fragment adjacent to the 2.8kb fragment described above was also found to encode essential functions that prevented the construction of chromosomal mutants by marker-exchange.

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Stinnett, Joshua. « The Chelation of Metal Ions by Vicibactin, a Siderophore Produced by Rhizobium leguminosarum ATCC 14479 ». Digital Commons @ East Tennessee State University, 2019. https://dc.etsu.edu/honors/485.

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Vicibactin is a small, high-affinity iron chelator produced by Rhizobium leguminosarum ATCC 14479. Previous work has shown that vicibactin is produced and secreted from the cell to sequester ferric iron from the environment during iron-deplete conditions. This ferric iron is then transported into the cell to be converted into ferrous iron. This study uses UV-Vis spectroscopy as well as ion trap-time of flight mass spectroscopy to determine that vicibactin does form a complex with copper(II) ions, however, at a much lower affinity than for iron(III). Stability tests have shown that the copper(II)-vicibactin complex is stable over time. The results of this study show that vicibactin could be used in order to remove copper(II) ions from the soil or other media if they are present in toxic amounts. It also suggests that vicibactin’s purpose for the rhizobia could be expanded to include both copper sequestering and to reduce extracellular copper concentrations to prevent toxicity.

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Cañete, Morales Alejandro Ignacio. « Caracterización molecular de aislados silvestres chilenos de Rhizobium a través del uso de marcadores moleculares basados en amplificación por RFLP-PCR ». Tesis, Universidad de Chile, 2007. http://www.repositorio.uchile.cl/handle/2250/101862.

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Barisic, Valeria. « Characterization of Putative ExbB and ExbD Leads to the Identification of a Potential Tol-Pal System in Rhizobium leguminosarum ATCC 14479 ». Digital Commons @ East Tennessee State University, 2015. https://dc.etsu.edu/etd/2489.

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Rhizobium leguminosarum is a Gram negative nitrogen-fixing soil bacterium. Due to the limited bioavailability of iron, bacteria utilize siderophores that scavenge and bind available iron. The transport of iron-siderophore complexes is achieved by the TonB-ExbB-ExbD complex. We have previously shown that a functional TonB protein is necessary for iron transport by creating ΔtonB mutants and assessing their growth and 55Fe-siderophore transport ability. We attempted to identify and characterize the roles of putative exbB and exbD genes using a similar approach. Growth curves and sequence analyses suggest putative exbB and exbD may be the tolpal-associated genes tolQ and tolR. Phenotypic and sensitivity assays showed mutants do not exhibit the characteristic tol phenotype and are not sensitive to detergents or changes in ionic strength of the growth medium. We also expressed and purified the 120 amino acid fragment of the TonB C-terminus for further physical and chemical characterization.

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Thaha, Fathuma Zuleikha. « Characterization of acetate metabolism genes in Sinorhizobium, Rhizobium, meliloti ». Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape7/PQDD_0016/MQ55093.pdf.

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Livres sur le sujet "Rhizobium leguminosarum Molecular aspects"

Rossen, Lone. Molecular analysis of the nodulation genes of "Rhizobium leguminosarum". Norwich : University of East Anglia, 1985.

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Sindhu, Satyavir Singh. Molecular analysis of lipopolysaccharide and membrane associated proteins in Rhizobium Leguminosarum. Norwich : University of East Anglia, 1990.

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Chapitres de livres sur le sujet "Rhizobium leguminosarum Molecular aspects"

Downie, J. A., B. P. Surin, I. J. Evans, L. Rossen, J. L. Firmin, C. A. Shearman et A. W. B. Johnston. « Nodulation Genes of Rhizobium Leguminosarum ». Dans Molecular genetics of plant-microbe interactions, 225–28. Dordrecht : Springer Netherlands, 1987. http://dx.doi.org/10.1007/978-94-009-4482-4_56.

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Colonna-Romano, S., R. Defez, M. Filser, M. Guida, M. Iaccarino, A. Lamberti, A. Riccio et al. « Glutamine Synthetases of Rhizobium Leguminosarum ». Dans Molecular genetics of plant-microbe interactions, 255–57. Dordrecht : Springer Netherlands, 1987. http://dx.doi.org/10.1007/978-94-009-4482-4_64.

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Hong, G. F., J. L. Burn et A. W. B. Johnston. « Analysis of the Mechanism of nod Gene Regulation in Rhizobium leguminosarum ». Dans Plant Molecular Biology, 523–30. Boston, MA : Springer US, 1987. http://dx.doi.org/10.1007/978-1-4615-7598-6_48.

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Ruiz-Argüeso, T., E. Hidalgo, J. Murillo, L. Rey et J. M. Palacios. « Molecular Genetics of the Hydrogen Uptake System of Rhizobium Leguminosarum ». Dans Advances in Molecular Genetics of Plant-Microbe Interactions Vol. 1, 222–25. Dordrecht : Springer Netherlands, 1991. http://dx.doi.org/10.1007/978-94-015-7934-6_34.

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Manian, S. S., P. Grönger, U. B. Priefer et A. Pühler. « Identification, Characterisation and Sequence Analysis of the Rhizobium Leguminosarum NifA Gene ». Dans Molecular genetics of plant-microbe interactions, 276–78. Dordrecht : Springer Netherlands, 1987. http://dx.doi.org/10.1007/978-94-009-4482-4_69.

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Vijn, Irma, Ton van Brussel, Albert van Kammen et Ton Bisseling. « The vetch (Vicia) and Rhizobium leguminosarum bv. viciae symbiosis : A system to study the activity of Rhizobium Nod factors. » Dans Plant Molecular Biology, 203–18. Berlin, Heidelberg : Springer Berlin Heidelberg, 1994. http://dx.doi.org/10.1007/978-3-642-78852-9_20.

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Sutton, J. M., R. Rivilla, A. E. Davies, E. J. A. Lea, S. Ghelani, C. Finnie, G. Dean et J. A. Downie. « Functional Analysis of Nodo and Nodt from Rhizobium Leguminosarum Biovar Viciae ». Dans Advances in Molecular Genetics of Plant-Microbe Interactions, 103–6. Dordrecht : Springer Netherlands, 1994. http://dx.doi.org/10.1007/978-94-011-0177-6_15.

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Tichy, H. V., C. Schild, H. M. Ripke, L. M. Nelson, H. Fees et W. Lotz. « Analysis of hup DNA and Hup host range of Rhizobium leguminosarum BIO ». Dans Molecular genetics of plant-microbe interactions, 279–81. Dordrecht : Springer Netherlands, 1987. http://dx.doi.org/10.1007/978-94-009-4482-4_70.

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Franssen, Henk J., Irma Vijn, Wei Cai Yang et Ton Bisseling. « Developmental aspects of the Rhizobium-legume symbiosis ». Dans 10 Years Plant Molecular Biology, 89–107. Dordrecht : Springer Netherlands, 1992. http://dx.doi.org/10.1007/978-94-011-2656-4_6.

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Spaink, Herman P., Guido V. Bloemberg, André H. M. Wijfjes, Tita Ritsema, Otto Geiger, Isabel M. López-Lara, Marga Harteveld et al. « The Molecular Basis of Host Specificity in the Rhizobium Leguminosarum-Plant Interaction ». Dans Advances in Molecular Genetics of Plant-Microbe Interactions, 91–98. Dordrecht : Springer Netherlands, 1994. http://dx.doi.org/10.1007/978-94-011-0177-6_13.

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Rapports d'organisations sur le sujet "Rhizobium leguminosarum Molecular aspects"

Ron, Eliora, et Eugene Eugene Nester. Global functional genomics of plant cell transformation by agrobacterium. United States Department of Agriculture, mars 2009. http://dx.doi.org/10.32747/2009.7695860.bard.

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The aim of this study was to carry out a global functional genomics analysis of plant cell transformation by Agrobacterium in order to define and characterize the physiology of Agrobacterium in the acidic environment of a wounded plant. We planed to study the proteome and transcriptome of Agrobacterium in response to a change in pH, from 7.2 to 5.5 and identify genes and circuits directly involved in this change. Bacteria-plant interactions involve a large number of global regulatory systems, which are essential for protection against new stressful conditions. The interaction of bacteria with their hosts has been previously studied by genetic-physiological methods. We wanted to make use of the new capabilities to study these interactions on a global scale, using transcription analysis (transcriptomics, microarrays) and proteomics (2D gel electrophoresis and mass spectrometry). The results provided extensive data on the functional genomics under conditions that partially mimic plant infection and – in addition - revealed some surprising and significant data. Thus, we identified the genes whose expression is modulated when Agrobacterium is grown under the acidic conditions found in the rhizosphere (pH 5.5), an essential environmental factor in Agrobacterium – plant interactions essential for induction of the virulence program by plant signal molecules. Among the 45 genes whose expression was significantly elevated, of special interest is the two-component chromosomally encoded system, ChvG/I which is involved in regulating acid inducible genes. A second exciting system under acid and ChvG/Icontrol is a secretion system for proteins, T6SS, encoded by 14 genes which appears to be important for Rhizobium leguminosarum nodule formation and nitrogen fixation and for virulence of Agrobacterium. The proteome analysis revealed that gamma aminobutyric acid (GABA), a metabolite secreted by wounded plants, induces the synthesis of an Agrobacterium lactonase which degrades the quorum sensing signal, N-acyl homoserine lactone (AHL), resulting in attenuation of virulence. In addition, through a transcriptomic analysis of Agrobacterium growing at the pH of the rhizosphere (pH=5.5), we demonstrated that salicylic acid (SA) a well-studied plant signal molecule important in plant defense, attenuates Agrobacterium virulence in two distinct ways - by down regulating the synthesis of the virulence (vir) genes required for the processing and transfer of the T-DNA and by inducing the same lactonase, which in turn degrades the AHL. Thus, GABA and SA with different molecular structures, induce the expression of these same genes. The identification of genes whose expression is modulated by conditions that mimic plant infection, as well as the identification of regulatory molecules that help control the early stages of infection, advance our understanding of this complex bacterial-plant interaction and has immediate potential applications to modify it. We expect that the data generated by our research will be used to develop novel strategies for the control of crown gall disease. Moreover, these results will also provide the basis for future biotechnological approaches that will use genetic manipulations to improve bacterial-plant interactions, leading to more efficient DNA transfer to recalcitrant plants and robust symbiosis. These advances will, in turn, contribute to plant protection by introducing genes for resistance against other bacteria, pests and environmental stress.

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